RU2293553C2 - Method for decreasing ammonia level in animal and human blood - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: it is necessary to introduce chemically pure glycerol for animals, moreover, before introduction it should be dissolved with distilled water at 1:1 ratio at the dosage of 2 ml/kg body weight, as for its introduction for people it is necessary to introduce 30 ml glycerol dissolved in 200 ml distilled water as a single dosage. The present innovation enhances transformation of highly toxic ammonia into intoxic compound - urea, that, in its turn, enables to reconstruct nitrogenous metabolism and improve health state in animals and people.

EFFECT: higher efficiency.

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Description

The invention relates to livestock (feeding farm animals), veterinary medicine and (internal diseases).

With improper and unbalanced feeding of farm animals and human nutrition, nitrogen metabolism is significantly impaired, accompanied by a sharp imbalance of free amino acids (M.V. Morgan et el., 1982, pp. 347-349), hyperaminoacidemia and hyperaminoaciduria (P. Dovat et el., 1975, p. 431-438), a strong increase in the level of ammonia (M. Imler et el., 1971, p. 320-321), a decrease in the activity of the urea cycle erythrinous enzymes. It is believed that nitrogenous coma and hepatic encephalopathy develop mainly on the basis of ammonia intoxication (M.V. Melk, 1979, pp. 58-62).

From the literature, analogues of lowering the level of ammonia in the blood in patients with chronic hepatitis and liver cirrhosis by the introduction of amino acids are known (M. Okita et el., 1981, pp. 210-413), carbohydrates (A. Frend et el., 1979, p. 261- 263, FLWeber et el., 1982, p. 316-317), hormones (J. B. Jospan et el., 1984, p. 117-119), calcium chloride solution (V. Guida et el, 1970, p. .329-331).

The prototype is a method of reducing toxic ammonia concentrations by oral administration of ornithine aspartate to a patient for two weeks, 3 times a day with a dose of 6 g. An aqueous solution is introduced. The clinical effectiveness of reducing ammonia in fasting blood by 34.6% is achieved after 2 weeks (R. Nilius, Klinikarzt No. 2/28, 1999, pp. 6-7).

The disadvantage of this method is that the decrease in the toxic concentration of ammonia after the introduction of the substance occurs after two weeks of use. A 34.6% decrease in the toxic ammonia concentration with the use of ornithine aspartate is insufficient, since in these liver diseases the level of ammonia in the blood exceeds the norm by 71%. The known method is not quite adequate: the introduction of amino acids can cause a violation of the synthesis of urea in the liver of patients, induce hepatic encephalopathy, act synergistically with ammonia, the effectiveness of the method is small.

The technical task of the invention is to reduce hyperammonioemia and enhance the transformation of highly toxic ammonia into a non-toxic compound urea.

The objective is achieved in a method of reducing the level of ammonia in the blood of animals and humans by oral administration of metabolically active compounds into the body, followed by determination of the concentration of ammonia in peripheral blood, where according to the invention, chemically pure glycerin is used as a metabolically active compound, a nitrogen-free non-carbohydrate compound, for which before administration glycerol is examined on an empty stomach, the content of ammonia in the blood, then glycerin, intended for introduction into the body, is diluted 1: 1 for animals with distilled water, a dose of 2 ml of trihydric alcohol per 1 kg of live weight, and for a person 30 ml of glycerol is diluted in 200 ml of distilled water for a single dose, then 3 hours after the introduction of glycerol, the blood is examined for ammonia content.

A significant difference of the invention is the use of a nitrogen-free substance - glycerol. The originality of the proposed method lies in the fact that a decrease in the toxic concentration of ammonia occurs immediately at 3 hours after the introduction of the substance. Moreover, the administration of glycerol to patients reduces the concentration of ammonia by 23.4% more than the administration of ornithine aspartate.

The implementation of the invention is carried out as follows: first, on an empty stomach, blood is tested for ammonia, then glycerol is orally administered in appropriate dilutions and after 3 hours the blood is again examined for ammonia.

Example 1. Cows of Simmental breed in the diet was added the enzyme preparation amylosubtilin G3x. Glycerin loading was carried out both during the control and during the experiment on days 1, 15, 30. The drug was prescribed in the diet for 30 days, at a dose of 300 g per 1 ton of feed. There was a change in the level of ammonia in the blood depending on the day of the study. And after loading with glycerin for 3 hours of blood testing, there was a decrease in the concentration of ammonia by an average of 17.5%.

Example 2. Cows of Simmental breed in the diet was added probiotic preparation lactobifid. The glycerol load was carried out both during the control and during the experiment on days 1, 15, 30, and 60 of the study. The drug was administered in the diet for 5 days at 5 doses per animal. There was a change in the level of ammonia in the blood depending on the day of the study. And after loading with glycerin for 3 hours of blood testing, there was a decrease in the concentration of ammonia by an average of 25.3%.

Example 3. The cows of Simmental breed in the diet was added biological product "Baikal-EM1." The glycerol load was carried out both during the control and during the experiment on days 1, 15, 30, and 60 of the study. The drug was prescribed in the diet for 30 days, at a dose of 50 ml per 1 animal per day. There was a change in the level of ammonia in the blood depending on the day of the study. And after loading with glycerin for 3 hours of blood testing, there was a decrease in the concentration of ammonia by an average of 24.6%.

Example 4. A patient, 46 years old, was admitted to the hospital with a diagnosis of chronic hepatitis with a transition to cirrhosis. Complaints at admission: pain and a feeling of heaviness in the right hypochondrium, ascites, dry mouth, lack of appetite, emaciation. The liver protrudes from under the costal arch by 8 cm. He considers himself a patient for a year, the disease associates with twice transmitted viral hepatitis.

On the second day after admission to the patient, a stress test with glycerin was performed. Before taking the drug, the blood ammonia content was 0.25 mmol / L. At 3 hours after applying a loading test of glycerol, the ammonia content was 0.12 mmol / L, a decrease of 52%.

Example 5. A patient, 50 years old, was admitted to the hospital with a diagnosis of chronic hepatitis in the acute stage. Associates his disease with the past malaria and subsequent alcohol abuse. The treatment is periodic, irregular. The patient was in the clinic for 22 days. Upon discharge on an empty stomach, the ammonia content in the blood was 0.28 mmol / L. After loading with glycerol, the concentration of this compound in the blood was 0.10 mmol / L; there was a decrease in the level of ammonia by 64%.

In total, we examined 68 healthy animals (Simmental cows) and 76 people. Of the people, 26 were practically healthy. Among patients with chronic hepatitis and cirrhosis of the liver, 50 people suffered.

Glycerin is an effective glucose precursor for dairy cows. In conditions of strengthening the process of glucose synthesis de novo, the ammonia content in the blood of cows after loading with glycerin decreased by an average of 22.5%.

In healthy people, with the intensification of the de novo glucose synthesis process, the ammonia content in the blood after glycerol loading decreased by an average of 26%.

In patients with chronic hepatitis, the concentration of ammonia in the blood was higher than normal by 71%. Three hours after the introduction of glycerol, the level of ammonia in the blood of patients decreased by an average of 58%.

Thus, the proposed method provides a decrease in the blood of animals and humans of a toxic concentration of ammonia. This method provides reliable detoxification of patients with chronic hepatitis and cirrhosis in different time parameters of the disease, significantly reducing their ammonia concentration.

Claims (1)

A method of reducing blood ammonia by orally administering metabolically active compounds to the body, characterized in that chemically pure glycerin is used as the metabolically active compound, which is diluted with distilled water 1: 1 for administration to animals and administered at a dose of 2 ml per 1 kg of live weight and a person is injected with 30 ml of glycerol diluted in 200 ml of distilled water for a single dose.