RU2010154103A - Композиции и способы, включающие варианты микробных протеаз - Google Patents
Композиции и способы, включающие варианты микробных протеаз Download PDFInfo
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- RU2010154103A RU2010154103A RU2010154103/10A RU2010154103A RU2010154103A RU 2010154103 A RU2010154103 A RU 2010154103A RU 2010154103/10 A RU2010154103/10 A RU 2010154103/10A RU 2010154103 A RU2010154103 A RU 2010154103A RU 2010154103 A RU2010154103 A RU 2010154103A
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- 239000000203 mixture Substances 0.000 title claims abstract 19
- 108091005804 Peptidases Proteins 0.000 title claims 2
- 102000035195 Peptidases Human genes 0.000 title claims 2
- 239000004365 Protease Substances 0.000 title claims 2
- 238000000034 method Methods 0.000 title claims 2
- 230000000813 microbial effect Effects 0.000 title 1
- 238000012986 modification Methods 0.000 claims abstract 22
- 230000004048 modification Effects 0.000 claims abstract 22
- 108090000787 Subtilisin Proteins 0.000 claims abstract 17
- 238000004140 cleaning Methods 0.000 claims abstract 17
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims abstract 12
- 108010056079 Subtilisins Proteins 0.000 claims abstract 12
- 102000005158 Subtilisins Human genes 0.000 claims abstract 12
- 239000003599 detergent Substances 0.000 claims abstract 7
- 238000005406 washing Methods 0.000 claims abstract 7
- 239000007788 liquid Substances 0.000 claims abstract 2
- 102200002697 rs1154510 Human genes 0.000 claims abstract 2
- 238000006467 substitution reaction Methods 0.000 claims abstract 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 108090000854 Oxidoreductases Proteins 0.000 claims 2
- 102000004316 Oxidoreductases Human genes 0.000 claims 2
- 229940088598 enzyme Drugs 0.000 claims 2
- 108010065511 Amylases Proteins 0.000 claims 1
- 102000013142 Amylases Human genes 0.000 claims 1
- 108700038091 Beta-glucanases Proteins 0.000 claims 1
- 102000005575 Cellulases Human genes 0.000 claims 1
- 108010084185 Cellulases Proteins 0.000 claims 1
- 108010023736 Chondroitinases and Chondroitin Lyases Proteins 0.000 claims 1
- 102000011413 Chondroitinases and Chondroitin Lyases Human genes 0.000 claims 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims 1
- 108090000371 Esterases Proteins 0.000 claims 1
- 108050009363 Hyaluronidases Proteins 0.000 claims 1
- 102000001974 Hyaluronidases Human genes 0.000 claims 1
- 108010029541 Laccase Proteins 0.000 claims 1
- 108090001060 Lipase Proteins 0.000 claims 1
- 102000004882 Lipase Human genes 0.000 claims 1
- 239000004367 Lipase Substances 0.000 claims 1
- 108090000128 Lipoxygenases Proteins 0.000 claims 1
- 102000003820 Lipoxygenases Human genes 0.000 claims 1
- 108700020962 Peroxidase Proteins 0.000 claims 1
- 102000003992 Peroxidases Human genes 0.000 claims 1
- 108010064785 Phospholipases Proteins 0.000 claims 1
- 102000015439 Phospholipases Human genes 0.000 claims 1
- 108010059820 Polygalacturonase Proteins 0.000 claims 1
- 108091007187 Reductases Proteins 0.000 claims 1
- 102000003425 Tyrosinase Human genes 0.000 claims 1
- 108060008724 Tyrosinase Proteins 0.000 claims 1
- 108090000637 alpha-Amylases Proteins 0.000 claims 1
- 108010084650 alpha-N-arabinofuranosidase Proteins 0.000 claims 1
- 235000019418 amylase Nutrition 0.000 claims 1
- 229940025131 amylases Drugs 0.000 claims 1
- 108010005400 cutinase Proteins 0.000 claims 1
- 238000004851 dishwashing Methods 0.000 claims 1
- 108010093305 exopolygalacturonase Proteins 0.000 claims 1
- 239000004744 fabric Substances 0.000 claims 1
- 235000013305 food Nutrition 0.000 claims 1
- 108010002430 hemicellulase Proteins 0.000 claims 1
- 108010059345 keratinase Proteins 0.000 claims 1
- 108010062085 ligninase Proteins 0.000 claims 1
- 235000019421 lipase Nutrition 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
- 108010038851 tannase Proteins 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/12—Soft surfaces, e.g. textile
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21062—Subtilisin (3.4.21.62)
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- General Preparation And Processing Of Foods (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
1. Вариант субтилизина, включающий, по меньшей мере, одну модификацию, где указанная модификация выбрана из: G100C, S101D, S101H, Q103D, M124G, L126G, S132H, S161N, Q275Z, и/или, по меньшей мере, из набора модификаций, выбранного из: ! ! ! ! ! ! ! ! и A142V/G211V, где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2. !2. Вариант субтилизина, включающий, по меньшей мере, одну модификацию, где указанная модификация выбрана из: ! ! и G097A/Z099.01S/G100S/G128A/Y217Q, где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2. ! 3. Вариант субтилизина, включающий, по меньшей мере, две модификации, где указанные модификации выбраны из: ! ! где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2. ! 4. Вариант субтилизина, включающий, по меньшей мере, две модификации, где указанные модификации выбраны из: ! ! где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2. ! 5. Вариант субтилизина, включающий замену Y217L и дополнительно включающий, по меньшей мере, одну модификацию по любому из пп.1-4, и где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2. ! 6. Вариант субтилизина, включающий замены G97A/G128A/Y217Q и дополнительно включающий, по меньшей мере, одну модификацию по любому из пп.1-4, и где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2. ! 7. Чистящая композиция, включающая, по меньшей мере, один вариант субтилизина по любому из пп.1-6. ! 8. Чистящая композиция по п.7, где указанная чистящая композиция является стирально-моющим средством. ! 9. Чистящая композиция по п.8, где указанное стирально-моющее средство является высокоэффективным жидким стирально-моющим средством. ! 10. Чистящая композиция по п.7, где указанная чистящая композиция являет�
Claims (16)
1. Вариант субтилизина, включающий, по меньшей мере, одну модификацию, где указанная модификация выбрана из: G100C, S101D, S101H, Q103D, M124G, L126G, S132H, S161N, Q275Z, и/или, по меньшей мере, из набора модификаций, выбранного из:
и A142V/G211V, где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2.
2. Вариант субтилизина, включающий, по меньшей мере, одну модификацию, где указанная модификация выбрана из:
и G097A/Z099.01S/G100S/G128A/Y217Q, где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2.
3. Вариант субтилизина, включающий, по меньшей мере, две модификации, где указанные модификации выбраны из:
где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2.
4. Вариант субтилизина, включающий, по меньшей мере, две модификации, где указанные модификации выбраны из:
где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2.
5. Вариант субтилизина, включающий замену Y217L и дополнительно включающий, по меньшей мере, одну модификацию по любому из пп.1-4, и где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2.
6. Вариант субтилизина, включающий замены G97A/G128A/Y217Q и дополнительно включающий, по меньшей мере, одну модификацию по любому из пп.1-4, и где положения соответствуют положениям субтилизина BPN' SEQ ID NO:2.
7. Чистящая композиция, включающая, по меньшей мере, один вариант субтилизина по любому из пп.1-6.
8. Чистящая композиция по п.7, где указанная чистящая композиция является стирально-моющим средством.
9. Чистящая композиция по п.8, где указанное стирально-моющее средство является высокоэффективным жидким стирально-моющим средством.
10. Чистящая композиция по п.7, где указанная чистящая композиция является средством для мытья посуды.
11. Чистящая композиция по любому из пп.7-10, дополнительно включающая один или более дополнительных ферментов или производных ферментов, выбранных из группы, состоящей из гемицеллюлаз, пероксидаз, протеаз, целлюлаз, ксиланаз, липаз, фосфолипаз, эстераз, кутиназ, пектиназ, кератиназ, редуктаз, оксидаз, фенолоксидаз, липоксигеназ, лигниназ, пуллуланаз, танназ, пентозаназ, маланаз, β-глюканаз, арабинозидаз, гиалуронидазы, хондроитиназы, лакказы и амилаз, или их смесей.
12. Чистящая композиция по п.11, дополнительно включающая, по меньшей мере, один стабилизатор.
13. Чистящая композиция, включающая, по меньшей мере, 0,0001 вес.%, по меньшей мере, одного варианта субтилизина по любому из пп.1-6 и необязательно, по меньшей мере, один подходящий дополнительный компонент.
14. Способ очистки, включающий стадии:
a) контакта поверхности и/или изделия, включающего ткань, с чистящей композицией по любому из пп.7-13; и
b) необязательно промывку и/или ополаскивание указанной поверхности или изделия.
15. Корм для животных, включающий, по меньшей мере, один вариант субтилизина по любому из пп.1-6.
16. Композиция для обработки продукта питания, включающая, по меньшей мере, один вариант субтилизина по любому из пп.1-6.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5969508P | 2008-06-06 | 2008-06-06 | |
| US61/059,695 | 2008-06-06 | ||
| PCT/US2009/046067 WO2009149145A2 (en) | 2008-06-06 | 2009-06-03 | Compositions and methods comprising variant microbial proteases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2010154103A true RU2010154103A (ru) | 2012-07-20 |
| RU2541786C2 RU2541786C2 (ru) | 2015-02-20 |
Family
ID=41009802
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2010154103/10A RU2541786C2 (ru) | 2008-06-06 | 2009-06-03 | Композиции и способы, включающие варианты микробных протеаз |
| RU2010153866/10A RU2010153866A (ru) | 2008-06-06 | 2009-06-03 | Композиции и способы, включающие в себя применение вариантных микробных протеаз |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2010153866/10A RU2010153866A (ru) | 2008-06-06 | 2009-06-03 | Композиции и способы, включающие в себя применение вариантных микробных протеаз |
Country Status (13)
| Country | Link |
|---|---|
| US (12) | US20110256610A1 (ru) |
| EP (10) | EP2647702A3 (ru) |
| JP (8) | JP6067221B2 (ru) |
| KR (3) | KR20110020245A (ru) |
| CN (7) | CN103382421B (ru) |
| AU (3) | AU2009256294A1 (ru) |
| BR (3) | BRPI0915073A2 (ru) |
| CA (3) | CA2726451A1 (ru) |
| DK (2) | DK2297314T3 (ru) |
| MX (5) | MX2010013100A (ru) |
| RU (2) | RU2541786C2 (ru) |
| WO (3) | WO2009149145A2 (ru) |
| ZA (1) | ZA201008169B (ru) |
Families Citing this family (127)
| Publication number | Priority date | Publication date | Assignee | Title |
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| DE60330346D1 (de) * | 2002-01-16 | 2010-01-14 | Genencor Int | Mehrfachsubstituierte proteasevarianten |
| US20090060933A1 (en) * | 2004-06-14 | 2009-03-05 | Estell David A | Proteases producing an altered immunogenic response and methods of making and using the same |
| JP6067221B2 (ja) * | 2008-06-06 | 2017-01-25 | ダニスコ・ユーエス・インク | 微生物プロテアーゼ変異種を含有する組成物と方法 |
| CN103122345A (zh) | 2008-11-11 | 2013-05-29 | 丹尼斯科美国公司 | 包含一种或多种可组合的突变的芽孢杆菌属枯草杆菌蛋白酶 |
| EP2589651A3 (en) | 2008-11-11 | 2013-08-28 | Danisco US Inc. | Compositions and methods comprising serine protease variants |
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| US20120252106A1 (en) * | 2009-09-25 | 2012-10-04 | Novozymes A/S | Use of Protease Variants |
| JP5863666B2 (ja) | 2009-12-09 | 2016-02-17 | ダニスコ・ユーエス・インク | プロテアーゼ変異体を含む組成物及び方法 |
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| MX336737B (es) * | 2010-05-06 | 2016-01-29 | Danisco Us Inc | Composiciones y metodos que comprenden variantes de proteasa serina. |
| EP3486319B1 (en) | 2011-05-05 | 2022-11-30 | Danisco US Inc. | Compositions and methods comprising serine protease variants |
| WO2012151480A2 (en) * | 2011-05-05 | 2012-11-08 | The Procter & Gamble Company | Compositions and methods comprising serine protease variants |
| US20140342433A1 (en) * | 2011-11-25 | 2014-11-20 | Novozymes A/S | Subtilase Variants and Polynucleotides Encoding Same |
| EP2607468A1 (en) * | 2011-12-20 | 2013-06-26 | Henkel AG & Co. KGaA | Detergent compositions comprising subtilase variants |
| US20140335596A1 (en) * | 2011-12-20 | 2014-11-13 | Novozymes A/S | Subtilase Variants and Polynucleotides Encoding Same |
| EP2628785B1 (en) * | 2012-02-17 | 2016-05-18 | Henkel AG & Co. KGaA | Detergent compositions comprising subtilase variants |
| WO2013120948A1 (en) * | 2012-02-17 | 2013-08-22 | Novozymes A/S | Subtilisin variants and polynucleotides encoding same |
| EP3354728B1 (en) | 2012-12-21 | 2020-04-22 | Danisco US Inc. | Alpha-amylase variants |
| WO2014099525A1 (en) | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Paenibacillus curdlanolyticus amylase, and methods of use, thereof |
| BR112015021647B1 (pt) | 2013-03-11 | 2023-04-25 | Danisco Us Inc | Variantes recombinantes de alfa-amilase e composições de detergente compreendendo as mesmas |
| WO2014194034A2 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
| JP6367930B2 (ja) | 2013-05-29 | 2018-08-01 | ダニスコ・ユーエス・インク | 新規メタロプロテアーゼ |
| EP3004341B1 (en) | 2013-05-29 | 2017-08-30 | Danisco US Inc. | Novel metalloproteases |
| EP3004314B1 (en) | 2013-05-29 | 2018-06-20 | Danisco US Inc. | Novel metalloproteases |
| WO2014207228A1 (en) | 2013-06-27 | 2014-12-31 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
| US20160145596A1 (en) | 2013-06-27 | 2016-05-26 | Novozymes A/S | Subtilase Variants and Polynucleotides Encoding Same |
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