RU115687U1 - PLASMA SAMPLING PIPPER STAND - Google Patents

Abstract

A stand for pipettes with wells for plasma samples, which is a rectangular structure 37.5 cm long, 6.0 cm high and 8.0 cm wide, made of a single piece of wood or plastic, on the upper surface of which there are two rows of holes: located at a distance of 2.9 cm from the front edge and 3.5 cm from the rear edge of the stand, it consists of 5 pairs of small cylindrical depressions (holes) with round walls 1.0 cm deep, 0.6 cm in diameter and a rounded bottom (radius 0.3 cm), located at a distance of 0.5 cm in each pair, at a distance from the side surfaces of the stand to the extreme holes of 3.0 cm, with a distance between pairs of holes 6.0 cm with gluing in front of each pair of grooves at a distance from them at 1.0 cm and at a distance of 1.5 cm from the front edge of the support, a piece of paper measuring 2.0 × 0.4 cm to mark the number of a specific plasma sample introduced into the recesses; the far row of holes in the stand, made behind small cylindrical recesses, consists of 10 cylindrical holes with a diameter of 1.0 cm, a depth of 5.0 cm and a flat bottom, spaced from the first row by 1.0 cm, from the rear edge of the stand by 1.5 cm, with a distance from the outermost cylindrical holes to the side surface of the stand of 1.0 cm and the arrangement of subsequent cylindrical holes in the row in pairs, with a distance between the holes in pairs of 2.0 cm and the distance between the outer holes and the first pair on either side and between pairs of holes by 3.7 cm.

Description

The proposed utility model relates to medical and biological technology, namely, devices that optimize the process of studying spontaneous aggregation of red blood cells (AE) in humans and animals.

The purpose of the utility model is to increase the laboratory working area and optimize the location of pipettes and wells with blood plasma of each individual biological object, eliminating their confusion during the assessment of AE, including out of the laboratory.

Analogues of the proposed utility model does not exist.

A stand is simple in design and accessible to use, on the upper surface of which there are openings arranged in two rows: ten cylindrical openings, which are nests for pipettes and ten small cylindrical recesses (holes), designed to obtain 1% erythrocyte hematocrit when they are resuspended after washing during the determination of spontaneous red blood cell aggregation.

As is known (Pakhrova O.A., Grineva M.R., Ivanov K.S. Methodology and clinical significance of the study of rheological properties of blood // Bulletin of the Ivanovo Medical Academy. - 2008. - T13, No. 1-2. - p. 89 -98.), To assess the spontaneous aggregation of red blood cells, blood is taken from a vein in the morning after 14 hours of fasting through a thick needle by gravity into a test tube with sodium citrate in a ratio of 9: 1 and centrifuged for 10 minutes. at 3000 rpm Typically, 2 wells of 0.2 ml of the subject's plasma are filled in a 96 well plate. All plasma and the white blood cell layer are removed from the tube. The red blood cells are resuspended in standard 1: 4 phosphate buffer, followed by centrifugation for 10 minutes. at 3 000 rpm./min., which allows them to be washed from the remains of the plasma when removing the supernatant. After that, 0.02 ml of red blood cells is taken and resuspended in the first autologous plasma-filled well of a 96 well plate, which allows to obtain 10% hematocrit. Then, 0.02 ml of the contents are taken from this well with a clean dry pipette and placed in a second filled well, which makes it possible to obtain 1% hematocrit. After that, 1 grid in the Goryaev’s chamber is filled with the obtained erythrocyte suspension, incubated for 3 minutes. for the occurrence of spontaneous aggregation, they count free red blood cells (including 2 red blood cells together) and aggregates starting with 3 red blood cells connected in the form of “coin columns”) in 2 large squares of the camera (lens × 40, eyepiece × 10 ) The number of “coin columns” and the number of red blood cells involved in them are considered.

The inventive stand is a rectangular structure (figure 1, figure 2, figure 3), length (1) 37.5 cm, height (2) 6.0 cm and width (3) 8.0 cm, made from a single piece of wood or plastic. On the upper surface of the stand (A) there are holes arranged in two rows. Closer to the front edge of the stand (B) at a distance of 2.9 cm from it there is a row consisting of 5 pairs of small cylindrical recesses (holes) (4) with round walls 1.0 cm deep, 0.6 cm in diameter and rounded bottom (radius 0.3 cm) located at a distance of 0.5 cm in each pair to place the plasma of the biological object under study and obtain a hematocrit of 10% and 1%. The distance from the rear edge of the stand (C) to small cylindrical recesses is 3.5 cm, from the side surfaces of the stand to the extreme recesses is 3.0 cm, the distance between the pairs of holes is 6.0 cm. At a distance of 1.0 cm from them closer to the front edge of the stand (B), and at a distance of 1.5 cm from it, before each pair of recesses, a piece of paper (5) is glued on with a size of 2.0 × 0.4 cm to mark the number of a specific sample of the currently studied blood plasma of a certain biological object. The second row is made behind small cylindrical recesses in the stand and consists of 10 cylindrical holes with a diameter of 1.0 cm, a depth of 5.0 cm and a flat bottom (6). The second row of holes is 1.0 cm away from the first, being 1.5 cm from the rear edge of the stand (C). The extreme cylindrical holes are 1.0 cm from the side surface of the stand. The subsequent cylindrical holes in the row, except the extreme ones, are in pairs. The distance between the holes in pairs is 2.0 cm, between the extreme holes and the first pair on either side, and between the pairs is 3.7 cm.

The stand can be used by placing pre-prepared clean pipettes with pears in each large opening, introducing them gradually into the small cylindrical recesses as they are being studied, diluting the washed red blood cells of the studied biological objects in it, according to the marking on the papers located on the top surface of the stand (5 ) to obtain ultimately 1% hematocrit needed to evaluate AE.

The stand seems simple and affordable to use, it allows you to optimize and streamline the process of assessing AE in humans and animals.

Claims (1)

Pipette holder with holes for plasma samples, which is a rectangular construction 37.5 cm long, 6.0 cm high and 8.0 cm wide, made of a single piece of wood or plastic, on the upper surface of which there are two rows of holes: the nearest, located at a distance of 2.9 cm from the front edge and 3.5 cm from the rear edge of the stand, consists of 5 pairs of small cylindrical recesses (holes) with round walls 1.0 cm deep, 0.6 cm in diameter and a rounded bottom (radius 0.3 cm), located at a distance of 0.5 cm in each pair, on distances from the side surfaces of the stand to the outer holes of 3.0 cm, with a distance between pairs of holes 6.0 cm with gluing in front of each pair of recesses at a distance of 1.0 cm from them and at a distance from the front edge of the stand of 1.5 cm paper the size of 2.0 × 0.4 cm for marking the number of a specific plasma sample made in the recesses; the distant row of holes in the stand, made behind small cylindrical recesses, consists of 10 cylindrical holes with a diameter of 1.0 cm, a depth of 5.0 cm and a flat bottom, spaced 1.0 cm from the first row, 1.5 times from the rear edge of the stand cm, when the distance from the extreme cylindrical holes to the side surface of the stand is 1.0 cm and the arrangement of subsequent cylindrical holes in a row in pairs, when the distance between the holes in pairs is 2.0 cm and the distance between the extreme holes and the first pair on either side and between pairs of holes P about 3.7 cm.