KR930700664A - 바실러스 튜링기네시스 cryIIIc 유전자와 콜레오프테란 곤충에 독성이 있는 단백질 - Google Patents
바실러스 튜링기네시스 cryIIIc 유전자와 콜레오프테란 곤충에 독성이 있는 단백질Info
- Publication number
- KR930700664A KR930700664A KR1019920702265A KR920702265A KR930700664A KR 930700664 A KR930700664 A KR 930700664A KR 1019920702265 A KR1019920702265 A KR 1019920702265A KR 920702265 A KR920702265 A KR 920702265A KR 930700664 A KR930700664 A KR 930700664A
- Authority
- KR
- South Korea
- Prior art keywords
- protein
- cholopteran
- toxic
- bacillus
- gene
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract 39
- 102000004169 proteins and genes Human genes 0.000 title claims abstract 31
- 241000238631 Hexapoda Species 0.000 title claims abstract 10
- 231100000331 toxic Toxicity 0.000 title claims 13
- 230000002588 toxic effect Effects 0.000 title claims 13
- 241000193830 Bacillus <bacterium> Species 0.000 title claims 8
- 101100061316 Bacillus thuringiensis cry7Aa gene Proteins 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 13
- 230000000749 insecticidal effect Effects 0.000 claims abstract 11
- 230000002708 enhancing effect Effects 0.000 claims abstract 3
- 239000004615 ingredient Substances 0.000 claims 8
- 241000894006 Bacteria Species 0.000 claims 5
- 230000000361 pesticidal effect Effects 0.000 claims 5
- 241000489975 Diabrotica Species 0.000 claims 3
- 101100061321 Bacillus thuringiensis subsp. japonensis cry8Ca gene Proteins 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 2
- 239000013612 plasmid Substances 0.000 claims 2
- 108091026890 Coding region Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 230000002147 killing effect Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
- A01N63/23—B. thuringiensis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Agronomy & Crop Science (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Catching Or Destruction (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (32)
- 제1도에 나열된 아미노산 서열을 코딩하고 있는 뉴클레오티드 염기서열을 가진 것을 특징으로 하는 경제 분리된 cryⅢC 유전자.
- 제1항에 있어서, 제1도에 나열된 뉴클레오티드 염기서열중 염기 14에서 1972까지로 이어진 코딩부위를 가진 유전자인 것을 특징으로 하는 cryⅢC 유전자.
- 제1항 또는 2항에 따른 유전자를 가지는 것을 특징으로 하는 재조합 플라스미드.
- 제1항 또는 2항에 따른 유전자에 의해 생성되는 것을 특징으로 하는 콜레오프테란 독성 단백질.
- 제3항에 따른 재조합 플라스미드로 형질도입된 것을 특징으로 하는 박테리아의 순수 배양물.
- 제5항에 있어서, 박테리아가 바실러스 튜링기네시스인 것을 특징으로 하는 박테리아 배양물.
- 제6항에 있어서, 바실러스가 튜링기네시스가 NRRL B-18627로 수락된 것을 특징으로 하는 박테리아 배양물.
- 농업적으로 이용가능한 수용체와 제4항에 따른 단백질로 구성된 것을 특징으로 하는 살충 성분물.
- 농업적으로 이용가능한 수용체와 제5항에 따른 박테리아와 이박테리아에서 생성되는 콜레오프테란 독성 단백질로 구성된 것을 특징으로 하는 살충 성분들.
- 제1항 또는 2항에 따른 유전자로 형질전환된 것을 특징으로 하는 식물.
- 제2항에 있어서, cryⅢC 유전자 또는 그 일부를 하이브리드프로브로 사용하기 위해 라벨링시키는 것을 특징으로 하는 cryⅢC 유전자.
- NRRL B-18533으로 기탁된 것을 특징으로 하는 바실러스 튜링기네시스 박테리아의 순수 배양물.
- 제1도에 나열된 아미노산 서열을 가지고 제12항에 따른 바실러스 튜링기네시스에 의해 생성되는 것을 특징으로 하는 콜레오프테란 독성 단백질.
- 농업적으로 이용가능한 수용체와 제13에 따른 콜레오프테란 독성단백질로 복합된 것을 특징으로 하는 살충 성분물.
- 제14항에 있어서, 바실러스 튜링기네시스내에 콜레오프테란 독성 단백질이 포함되어 있는 것을 특징으로 하는 살충 성분물.
- 제4항에 따른 콜레오프테란 독성 단백질의 살충 효과량으로 콜레오프테란 곤충의 숙주식물에 살포하는 것으로 구성된 콜레오프테란 곤충의 조절방법.
- 제16항에 있어서, 콜레오프테란 독성 단백질이 바실러스 튜링기네시스내에 포함되어 있는 것을 특징으로 하는 조절방법.
- 제16항에 있어서, 곤충이 디아브로티카종인 것을 특징으로 하는 방법.
- 제13항에 따른 콜레오프테란 독성 단백질 효과량을 곤충의 숙주식물에 효과량을 곤충의 숙주식물에 살포하는 것으로 구성된 것을 특징으로 하는 콜레오프테란 곤충 조절방법.
- 제19항에 있어서, 콜레오프테란 독성 단백질이 바실러스 튜링게네시스에 포함되는 것을 특징으로 하는 방법.
- 제19항에 있어서, 곤충은 디아브로티카종인 것을 특징으로 하는 방법.
- 클레오프테란 곤충에 대해 성분물의 살충활성을 강화하기 위해 cryⅠ 단백질 효과량의 cryⅢC 단백질로 구성된 콜레오프테란 독성 단백질의 살충활성을 강화하는 것을 특징으로 하는 방법.
- 제22항에 있어서, cryⅢC 단백질이 cryⅢC 단백질인 것을 특징으로 하는 방법.
- 제23항에 있어서, cryⅠ 단백질이 cryⅠA 단백질인 것을 특징으로 하는 방법.
- 제23항에 있어서, cryⅠ 단백질이 cryⅠA(c) 단백질인 것을 특징으로 하는 방법.
- 제22항에 있어서, cryⅢ 단백질과 cryⅠ 단백질은 동량이 존재하는 것을 특징으로 하는 방법.
- 제22 내지 26항에 있어서, 성분물은 디아브로티카종에 대해 살충활성이 강화된 것을 특징으로 하는 방법.
- 콜레오프테란 곤충에 대해 살출활성이 강화시키기 위해 cryⅠ 단백질과 제13항에 따른 콜레오프테란 독성단백질로 구성된 것을 특징으로 하는 콜레오프테란에 유용한 살충 성분물.
- 제28항에 있어서, cryⅠ 단백질이 cryⅠA 단백질인 것을 특징으로 하는 살충성분물.
- 제28항에 있어서, cryⅠ 단백질이 cryⅠA(c) 단백질인 것을 특징으로 하는 살충성분물.
- 제28항에 있어서, 콜레오프테란 독성 단백질과 cryⅠ 단백질이 동량이 존재하는 것을 특징으로 하는 살충 성분물.
- ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US496,568 | 1990-03-20 | ||
US07/496,568 US5187091A (en) | 1990-03-20 | 1990-03-20 | Bacillus thuringiensis cryiiic gene encoding toxic to coleopteran insects |
PCT/US1991/001786 WO1991014778A2 (en) | 1990-03-20 | 1991-03-18 | BACILLUS THURINGIENSIS cryIIIC GENE AND PROTEIN TOXIC TO COLEOPTERAN INSECTS |
Publications (1)
Publication Number | Publication Date |
---|---|
KR930700664A true KR930700664A (ko) | 1993-03-15 |
Family
ID=23973206
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019920702265A KR930700664A (ko) | 1990-03-20 | 1991-03-18 | 바실러스 튜링기네시스 cryIIIc 유전자와 콜레오프테란 곤충에 독성이 있는 단백질 |
Country Status (14)
Country | Link |
---|---|
US (2) | US5187091A (ko) |
EP (2) | EP0606110A1 (ko) |
JP (1) | JP3078576B2 (ko) |
KR (1) | KR930700664A (ko) |
AT (1) | ATE125569T1 (ko) |
AU (1) | AU645080B2 (ko) |
CA (1) | CA2078571C (ko) |
DE (1) | DE69111600T2 (ko) |
ES (1) | ES2077223T3 (ko) |
HU (1) | HUT62036A (ko) |
NZ (1) | NZ237404A (ko) |
PL (1) | PL165919B1 (ko) |
RU (1) | RU2106409C1 (ko) |
WO (1) | WO1991014778A2 (ko) |
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-
1991
- 1991-03-12 NZ NZ237404A patent/NZ237404A/en unknown
- 1991-03-18 RU SU5053184A patent/RU2106409C1/ru active
- 1991-03-18 EP EP94102819A patent/EP0606110A1/en not_active Withdrawn
- 1991-03-18 HU HU9202984A patent/HUT62036A/hu unknown
- 1991-03-18 KR KR1019920702265A patent/KR930700664A/ko not_active Application Discontinuation
- 1991-03-18 JP JP03506608A patent/JP3078576B2/ja not_active Expired - Lifetime
- 1991-03-18 DE DE69111600T patent/DE69111600T2/de not_active Expired - Lifetime
- 1991-03-18 EP EP91907293A patent/EP0522036B1/en not_active Expired - Lifetime
- 1991-03-18 ES ES91907293T patent/ES2077223T3/es not_active Expired - Lifetime
- 1991-03-18 WO PCT/US1991/001786 patent/WO1991014778A2/en active IP Right Grant
- 1991-03-18 CA CA002078571A patent/CA2078571C/en not_active Expired - Lifetime
- 1991-03-18 AU AU75552/91A patent/AU645080B2/en not_active Expired
- 1991-03-18 AT AT91907293T patent/ATE125569T1/de not_active IP Right Cessation
- 1991-03-20 PL PL91289512A patent/PL165919B1/pl unknown
-
1992
- 1992-09-24 US US07/950,352 patent/US5382429A/en not_active Expired - Lifetime
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CA2078571C (en) | 2004-06-01 |
US5187091A (en) | 1993-02-16 |
WO1991014778A3 (en) | 1991-12-12 |
WO1991014778A2 (en) | 1991-10-03 |
NZ237404A (en) | 1992-06-25 |
ES2077223T3 (es) | 1995-11-16 |
JP3078576B2 (ja) | 2000-08-21 |
US5382429A (en) | 1995-01-17 |
JPH05505109A (ja) | 1993-08-05 |
AU645080B2 (en) | 1994-01-06 |
PL165919B1 (pl) | 1995-03-31 |
ATE125569T1 (de) | 1995-08-15 |
DE69111600T2 (de) | 1996-02-29 |
HUT62036A (en) | 1993-03-29 |
EP0606110A1 (en) | 1994-07-13 |
PL289512A1 (en) | 1991-12-16 |
AU7555291A (en) | 1991-10-21 |
RU2106409C1 (ru) | 1998-03-10 |
CA2078571A1 (en) | 1991-09-21 |
EP0522036A1 (en) | 1993-01-13 |
EP0522036B1 (en) | 1995-07-26 |
DE69111600D1 (en) | 1995-08-31 |
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