KR20240068864A - Composition of tick repellent agent and tick repellent manufactured by the composition - Google Patents

Abstract

The present invention relates to a composition for repelling ticks and a tick repellent manufactured using the same. More specifically, a composition for repelling ticks containing pyrethrum extract and water-soluble phytoncide as active ingredients, and a tick repellent manufactured using the same. It's about.
This patent is the result of support from the Busan Metropolitan City and Silla University Innovation Research Complex (I-URP) development project.

Description

Composition for tick repellent and tick repellent manufactured using the same {COMPOSITION OF TICK REPELLENT AGENT AND TICK REPELLENT MANUFACTURED BY THE COMPOSITION}

The present invention relates to a composition for repelling ticks and a tick repellent manufactured using the same. More specifically, a composition for repelling ticks containing pyrethrum extract and water-soluble phytoncide as active ingredients, and a tick repellent manufactured using the same. It's about.

Pyrethrum is a plant in the Asteraceae family that has white petals and a yellow center. The plant and flower parts of pyrethrum contain a light red-yellow oil-like substance called pyrethrin, which is highly toxic to cold-blooded animals and insects, but is not toxic to warm-blooded animals, making it suitable as a high-quality household insect repellent.

Phytoncide refers to volatile aromatic components obtained by distilling plants with steam. Volatile components consist of dozens to over 200 types of compounds such as phenolics, terpenoids, alkanoids, phenylpropane, aceptogenin, and steroids. Among them, cypress is a large evergreen tree that is native to Japan and belongs to the Cypress genus of the Cupressaceae family of the Arborvitae family of the Cypress family. The cypress tree produces phytoncide, a natural chemical substance, to protect itself from pests such as external mites, and the essential oil processed from it is said to be effective in exterminating house dust mites because it has excellent deodorizing and antibacterial properties.

Due to COVID-19, the number of households seeking to raise pets has increased as many companies have shifted their employees to work remotely or from home. According to data from the 2021 National Awareness Survey on Animal Protection and Welfare by the Korea Institute of Agriculture, Food, and Rural Affairs, the rate of raising companion animals in 2021 was 28.5%, an increase of 0.8 percentage points compared to 27.7% in 2020. According to Statistics Korea's 2020 population and housing survey sample count, the number of households owning companion animals is 3,129 thousand households (15.0%), the number of households owning dogs is 2,423 thousand households (11.6%), and the number of households owning cats is 717 thousand households (3.4%). ), the proportion of households raising dogs was found to be 8.2%p higher. The most common reason for owning a pet is that it provides emotional stability and happiness as a member of the family. As such, many people experience a significant psychological impact while raising pets. However, in addition to problems such as noise, defecation, and smell caused by raising pets, there is a possibility of contracting zoonotic diseases (zoonoses) through pets.

Ticks are ectoparasites that suck blood to obtain nutrients from vertebrates, and are a major vector of infectious diseases that live in the human body and transmit pathogens such as viruses, bacteria, rickettsiae, and parasites.

Ticks, one of the blood-sucking arthropods, are animals that spread infectious diseases by sucking blood from various animals as well as people, or cause stress such as sleep disturbance, weight loss, and anemia through direct blood sucking, and are also caused by hairy mites, soft mites, mosquitoes, and flies. , backs, bedbugs, mites, etc., cause diseases by transmitting many infectious pathogens to humans and animals.

Diseases transmitted by ticks include tick-borne typhus, anaplasmosis, tick-borne encephalitis, ehrlichiosis, tularemia, and barbes. These include Babesiosis, Theileriasis, and Lyme disease. Therefore, many tick insecticides are sold on the market to prevent diseases transmitted by ticks.

However, conventional mite pesticides cause side effects such as skin rash, itching, convulsions, and seizures. In addition, certain ingredients in tick pesticides, such as Glyphosate, Malathion, Diazinon, Tetrachlorvinphos, and Parathion, act as fatal carcinogens to animals and humans. do.

Therefore, since pesticides have a negative effect on people, animals, and the environment due to their toxicity, the need for natural repellents rather than chemical pesticides is increasing.

Republic of Korea Patent Publication No. 10-0341056

The purpose of the present invention is to provide a composition for repelling ticks that has excellent tick repellent effect without adversely affecting people, animals, and the environment.

Another object of the present invention is to provide a tick repellent manufactured using the tick repellent composition.

The technical problems to be achieved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those skilled in the art from the description of the present invention.

In order to achieve the above object, the present invention provides a tick repellent composition and a tick repellent manufactured using the same.

Hereinafter, this specification will be described in more detail.

The present invention provides a composition for repelling ticks containing pyrethrum extract, water-soluble phytoncide, polysorbate 80, and 1,2-hexanediol as active ingredients.

In the present invention, the pyrethrum extract is included in an amount of 0.5 to 1.5 parts by weight relative to the total weight of the composition, the water-soluble phytoncide is included in an amount of 0.5 to 1.5 parts by weight relative to the total weight of the composition, and the polysorbate 80 and 1,2-hexanediol ( 1,2-Hexanediol) is characterized in that it is included in an amount of 0.5 to 1.5 parts by weight and 1 to 2 parts by weight, respectively, based on the total weight of the composition.

The present invention provides a tick repellent prepared using a tick repellent composition containing the above pyrethrum extract, water-soluble phytoncide, polysorbate 80, and 1,2-hexanediol as active ingredients. .

In the present invention, the tick is characterized in that it includes one or more species selected from the small skin tick, the dog skin tick, the blunt tick, and the Japanese tick.

In the present invention, the repellent is characterized in that it is used in the form of one or more formulations selected from the group consisting of liquid, emulsion, coating agent, smoke agent, fumigant, granule, and solid agent.

All matters mentioned in the tick repellent composition and the tick repellent manufactured using the composition apply equally unless contradictory.

The composition for tick repellent containing pyrethrum extract and water-soluble phytoncide as active ingredients according to the present invention can produce a tick repellent with excellent tick repellent effect without adversely affecting people, animals, and the environment.

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

Figure 1 is a graph showing the tick repellent effect of the pyrethrum extract according to the present invention using a tick climbing test.
Figure 2 is a graph showing the tick-repelling effect of the water-soluble phytoncide according to the present invention using a tick climbing test.
Figure 3 is a graph showing the tick-repelling effect of a composition containing pyrethrum extract and water-soluble phytoncide according to the present invention using a tick climbing test.
Figure 4 is a graph showing the tick repellent effect of a composition containing pyrethrum extract and water-soluble phytoncide according to the present invention using an indoor repellent efficacy test method.

The terms used in this specification are general terms that are currently widely used as much as possible while considering the function in the present invention, but this may vary depending on the intention or precedent of a person working in the art, the emergence of new technology, etc. In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the relevant invention. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than simply the name of the term.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms defined in commonly used dictionaries should be interpreted as having meanings consistent with the meanings they have in the context of the related technology, and unless clearly defined in the present application, should not be interpreted in an ideal or excessively formal sense. No.

The numerical range includes the values defined in the range above. Any maximum numerical limit given throughout this specification includes all lower numerical limits as if the lower numerical limit were clearly written. Every minimum numerical limit given throughout this specification includes every higher numerical limit as if such higher numerical limit was clearly written. All numerical limits given throughout this specification will include all better numerical ranges within the broader numerical range, as if the narrower numerical limits were clearly written.

Hereinafter, the present invention will be described in detail.

The present inventor confirmed the mite repellent effect of each of the pyrethrum extract or water-soluble phytoncide, and the mite repellent effect of the composite containing both the pyrethrum extract and water-soluble phytoncide was superior to that of the pyrethrum extract or water-soluble phytoncide, respectively. By confirming that it was effective, the present invention was completed.

The present invention provides a composition for repelling ticks containing pyrethrum extract, water-soluble phytoncide, polysorbate 80, and 1,2-hexanediol as active ingredients.

The pyrethrum extract may be included in an amount of 0.5 to 1.5 parts by weight, preferably 0.7 to 1.2 parts by weight, based on the total weight of the composition. The water-soluble phytoncide may be included in an amount of 0.5 to 1.5 parts by weight, preferably 0.7 to 1.2 parts by weight, based on the total weight of the composition. The polysorbate 80 may be included in an amount of 0.5 to 1.5 parts by weight, preferably 0.7 to 1.2 parts by weight, based on the total weight of the composition. The 1,2-hexanediol may be included in an amount of 1 to 2 parts by weight, preferably 1.2 to 1.7 parts by weight, based on the total weight of the composition.

The present invention provides a tick repellent prepared using a tick repellent composition containing pyrethrum extract, water-soluble phytoncide, polysorbate 80, and 1,2-hexanediol as active ingredients.

The “mite” belongs to blood-sucking ectoparasites and may include, but is not limited to, one or more species selected from the small skin tick, the dog skin tick, the blunt tick, and the Japanese tick.

The “repellent” refers to a drug with the purpose of preventing harm from pests such as mosquitoes, ticks, and lice, and may be used in the form of one or more formulations selected from the group consisting of liquids, emulsions, liniments, smokers, fumigants, granules, and solids. However, it is not limited to this.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited to the following examples.

Example 1. Preparation of a composition for repelling ticks containing pyrethrum extract and water-soluble phytoncide

Pyrethrum was used by purchasing Pyrethrum (EW), a 5% water-based concentrated emulsion derived from pyrethrum residue-free natural chrysanthemum plants sold by Kast Life and Environmental Science Research Institute, and water-soluble phytoncide was obtained by distillation extraction method from Fine2, Tokyo, Japan. Phytoncide was purchased and used.

A composition for repelling ticks by mixing 1 part by weight of the pyrethrum extract, 1 part by weight of the water-soluble phytoncide, 1 part by weight of polysorbate 80, 1.5 parts by weight of 1,2-hexanediol, and 95.5 parts by weight of purified water. was manufactured. The components and parts by weight of the composition are listed in Table 1.

Experimental Example 1. Tick repellent effect of pyrethrum extract

A tick climbing test was conducted to determine the effectiveness in repelling ticks. Small ticks were collected by flagging using a white cloth measuring 1 m wide and 1 m tall from the walkway next to Gangnaru Reed Square in Samnak Park, 29-1 Samnak-dong, Sasang-gu, Busan. The small ticks collected on the same day were collected in the laboratory. After moving to , only active small ticks were carefully selected and prepared.

100 μl of pyrethrum extract was evenly applied to the filter paper and dried for 10 minutes. While the filter paper was drying, a 50 ml conical tube was prepared by making a hole in the bottom with a syringe. The dried filter paper was fixed on the top of the perforated conical tube, and the 10 collected mites were inserted into the hole of the conical tube, and the positions of the mites in the tube were observed.

The positions of 10 small ticks were measured and recorded at 10-minute intervals for a total of 1 hour, and location scores were calculated according to the measured positions. The position score was set according to the height by dividing the conical tube into 10 equal parts, the bottom point was set as 1 point, and the top where the filter paper is located was set as 10 points. The tick repellent effect score was calculated by the method of Equation 1 below, where 10 is the lowest score and 100 is the highest score. The lower the score, the greater the repellent effect, and the higher the score, the smaller the repellent effect.

[Equation 1]

Comparative Example 1. Control group to determine the tick repellent effect of pyrethrum extract and water-soluble phytoncide

Comparative Example 1 was conducted to determine the effectiveness of pyrethrum extract and water-soluble phytoncide in repelling ticks. Comparative Example 1 was conducted in the same manner as Experimental Example 1, except that empty filter paper was used instead of the filter paper coated with the pyrethrum extract.

The tick repellent effect of the pyrethrum extract confirmed in Experimental Example 1 and Comparative Example 1 is listed in Table 2. Experimental Example 1 scored 23 points on average, and Comparative Example 1 scored 65.5 points on average. Therefore, it was confirmed that the pyrethrum extract has a tick repellent effect. The tick repellent effect of the pyrethrum extract is shown graphically in Figure 1.

Experimental Example 2. Tick repellent effect of water-soluble phytoncide

Experimental Example 2 was conducted to determine the effect of water-soluble phytoncide in repelling ticks. Experimental Example 2 was conducted in the same manner as Experimental Example 1, except that filter paper coated with water-soluble phytoncide was used instead of filter paper coated with the pyrethrum extract.

The tick repellent effect of the water-soluble phytoncide confirmed in Experimental Example 2 and Comparative Example 1 is listed in Table 3. Experimental Example 2 scored 22 points on average, and Comparative Example 1 scored 65.5 points on average. Therefore, it was confirmed that the water-soluble phytoncide has a tick repellent effect. The effect of the water-soluble phytoncide in repelling ticks is shown graphically in Figure 2.

Experimental Example 3. Tick repellent effect of a tick repellent composition containing pyrethrum extract and water-soluble phytoncide

Experimental Example 3 was conducted to determine the tick repellent effect of a tick repellent composition containing pyrethrum extract and water-soluble phytoncide. Experimental Example 3 was conducted in the same manner as Experimental Example 1, except that filter paper coated with the mite repellent composition prepared in Example 1 was used instead of the filter paper coated with the pyrethrum extract. In addition, the effectiveness of the composition for repelling ticks prepared in Example 1 was compared with products from other companies.

The tick repellent effect of the tick repellent composition confirmed in Experimental Example 3 is listed in Table 4. Experimental Example 3 scored 13 points on average, products from other companies scored 26.67 points, and Comparative Example 1 scored 65.5 points on average. Therefore, it was confirmed that the composition for repelling ticks was effective in repelling ticks, and it was confirmed that it had a better tick repellent effect compared to products from other companies. The tick repellent effect of the tick repellent composition is shown graphically in Figure 3.

According to Experimental Examples 1 to 3 and Comparative Example 1, each of pyrethrum and water-soluble phytoncide had a tick-repelling effect, but the tick-repelling composition prepared by combining both pyrethrum and water-soluble phytoncide had a better tick-repelling effect. It was confirmed that it had a repellent effect.

Experimental Example 4. Tick repellent effect of a tick repellent composition containing pyrethrum extract and water-soluble phytoncide

To determine the tick repellent effect of a tick repellent composition containing pyrethrum extract and water-soluble phytoncide, an indoor tick repellent efficacy test was performed. Small ticks were collected by flagging using a white cloth measuring 1 m wide and 1 m tall from the walkway next to Gangnaru Reed Square in Samnak Park, 29-1 Samnak-dong, Sasang-gu, Busan. The small ticks collected on the same day were collected in the laboratory. After moving to , only active small ticks were carefully selected and prepared.

The repellent zone (RZ) was set as a circle with a diameter of 6 cm based on the center of the filter paper, and the active zone (AZ) was set as a circle with a diameter of 3.2 cm based on the center of the filter paper. The tick repellent composition prepared in Example 1 was applied to the RZ of the filter paper and dried for 2 minutes at a temperature of 24°C and humidity of 30%. Five small ticks were placed in the AZ of the dried filter paper for 2 minutes, and after 2 minutes, ticks that escaped the RZ were excluded and the final number of ticks present in the AZ was confirmed. The tick evasion rate was calculated using Equation 2 below. The same method as above was applied to compare the tick repellent effects of the tick repellent composition prepared in Example 1 and that of other companies' products.

[Equation 2]

The results of the indoor tick repellent efficacy test method confirmed in Experimental Example 4 are listed in Table 5. When the number of ticks outside the RZ of Table 5 was calculated using Equation 2, the tick repellent composition of the present invention recorded a repellent rate of 88%, and the other company's product recorded a repellent rate of 12%. Therefore, it was confirmed that the composition for repelling ticks of the present invention has a tick repellent effect and that it has a better tick repellent effect compared to products of other companies. The tick repellent rate of the tick repellent composition is shown graphically in Figure 4.

Experimental Example 5. Deodorizing performance test of a composition for tick repellent containing pyrethrum extract and water-soluble phytoncide

A sample nozzle for spraying the deodorant sample and a gas collection port for collecting the gas in the sealed container were attached, and a test container with a volume of 50 L made of glass was prepared. The closed container was maintained at a temperature of (20.6 ± 0.2) °C and relative humidity (55.0 ± 0.5) % R.H. The initial concentration of odor components in the test container was 100 ppm of ammonia and 30 ppm of trimethylamine.

The composition for repelling ticks prepared in Example 1 was sprayed into the sample nozzle of the test container. At 30 minutes after injection, the gas in the test container was collected and the concentration (C _t,i ) of the odor component was measured.

Comparative Example 2. Control group to determine the deodorizing performance effect of pyrethrum extract and water-soluble phytoncide

Comparative Example 2 was conducted in the same manner as Experimental Example 5, except that water, rather than a tick repellent composition, was sprayed onto the sample sprayed into the test container. At 30 minutes after injection, the gas in the test container was collected and the odor component concentration (C _b,i ) was measured.

The odor component concentration reduction rate was calculated using the odor component concentrations (C _t,i , C _b,i ) measured in Experimental Example 5 and Comparative Example 2, and the odor component concentration reduction rate was calculated using Equation 3 below.

[Equation 3]

Experimental Example 6. Antibacterial performance test of a composition for tick repellent containing pyrethrum extract and water-soluble phytoncide

In Experimental Example 6, the experiment was conducted by dividing the experimental group into Blank and Sample. In the Blank group, 99 ml of water and 1 ml of the inoculum strain were added to an Erlenmeyer flask, and cultured with shaking at 37°C for 24 hours. In the Sample group, 99 ml of the tick repellent composition prepared in Example 1 was placed in an Erlenmeyer flask. After adding 1 ml of the inoculum strain, it was cultured with shaking at 37°C for 24 hours.

After cultivating the Blank group and the Sample group with shaking for 24 hours, the bacteriostatic reduction rate and sterilization reduction rate of the composition for repelling ticks were calculated. The bacteriostatic reduction rate was calculated using Equation 4 below, and the bactericidal reduction rate was calculated using Equation 5 below.

[Equation 4]

[Equation 5]

(In Equations 4 to 5 above,

A: Sample concentration immediately after adding the inoculum strain in the blank group;

B: Sample concentration 24 hours after adding the inoculum strain in the blank group;

C: refers to the sample concentration 24 hours after adding the inoculum strain in the sample group;)

In Experimental Example 6, Escherichia coli, Staphylococcus aureus, or Klebsieila pneumoniae were used as the inoculum strains, respectively, and the results of Experimental Example 6 are shown in Table 6. As a result of Experimental Example 6, the bacteriostatic reduction rate of the composition for repelling ticks of the present invention was 99.9% in all E. coli, Staphylococcus aureus, and Pneumococci, and the bactericidal reduction rate was also 99.9% in E. coli, Staphylococcus aureus, and Pneumococci. Therefore, the antibacterial effect of the composition for repelling ticks of the present invention was confirmed.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive.

Claims (5)

A composition for repelling ticks containing pyrethrum extract, water-soluble phytoncide, polysorbate 80, and 1,2-hexanediol as active ingredients.
According to paragraph 1,
The pyrethrum extract is
It is included in 0.5 to 1.5 parts by weight relative to the total weight of the composition,
The water-soluble phytoncide is
It is included in 0.5 to 1.5 parts by weight relative to the total weight of the composition,
The polysorbate 80 and 1,2-hexanediol are
A composition for repelling ticks, characterized in that it contains 0.5 to 1.5 parts by weight and 1 to 2 parts by weight, respectively, based on the total weight of the composition.
A tick repellent manufactured using the composition of claim 1 or 2.
According to any one of claims 1 to 3,
The tick is
A composition for repelling ticks, comprising one or more species selected from small ticks, ticks, ticks, and Japanese ticks.
According to paragraph 3,
The repellent
A repellent, characterized in that it is used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coating agents, smoking agents, fumigants, granules, and solid agents.