KR20230015110A - Thermostatic device for laboratory container - Google Patents

Abstract

Disclosed is a thermostatic device for a laboratory container. The thermostatic device for a laboratory container comprises: a container unit having a space therein to accommodate the laboratory container; a heating wire provided to surround the container unit and generating heat to transfer the heat to the container; and a cover unit closing an open portion on the upper part of the container unit to prevent the heat in the space inside the container unit from being released to the outside.

Description

Laboratory container thermostat {THERMOSTATIC DEVICE FOR LABORATORY CONTAINER}

The present invention relates to a laboratory vessel thermostat, and more specifically, to a laboratory vessel thermostat capable of maintaining a constant temperature of a laboratory vessel accommodating cells and a culture medium.

In general, the ratio of biopharmaceuticals produced using animal cell lines, including CHO cells, is growing rapidly every year, and about 70% of biopharmaceuticals approved for manufacturing by certification bodies such as the US FDA and Korea's Ministry of Food and Drug Safety use animal cell lines. It is known to be produced using

Usually, about 40% of biopharmaceuticals approved for manufacturing contain glycosylation. The glycosylation process is known to play an important role in the efficacy of pharmaceuticals, the stability of proteins themselves, and the half-life in blood. In order for such a glycosylation process to be properly expressed, animal cell lines, not cell lines such as E. coli and yeast, must be used. In addition, it has been expanded from simple monoclonal antibody (mAb) to bispecific antibody (bsAb) and fusion protein, and materials that have been proven safe through global pharmaceutical companies (ex. PEG ) The development of convergence biopharmaceuticals is being actively carried out.

On the other hand, in culturing these animal cell lines, the most important thing is the culture medium (Media). The culture medium is very involved in the viability and productivity of the entire cell. Since this is influenced by the components of the culture medium, many studies are being conducted to develop a better culture medium, but no matter how good the culture medium is, if the appropriate temperature for the cell line is not maintained, the cells will suffer from cold shock. It can be destroyed, which is a very big problem.

Usually, in the case of a laboratory at a corporate level, when cell culture, subculture, etc. are performed, a small water bath is placed inside the clean bench so that the temperature of the culture medium is kept constant. However, since such a small water bath costs at least 500,000 won or more, it is difficult to purchase an extra in a small school unit laboratory.

Because the clean bench must always be kept aseptic, wind always blows out to prevent outside impurities from entering the inside, so if you put the culture medium inside the clean bench, the temperature cools down quickly. do. Experimenters who are not familiar with the experiment may make mistakes such as contaminating the flask in which the cells are cultured by mistake while paying attention to the temperature, and in this case, there is a problem in that it takes about 2 weeks or more to re-culture the cells.

On the other hand, as described above, in order to maintain a constant temperature of the culture medium, an 'experimental incubation device (Korea Utility Model Office 0127676)' has been proposed.

The conventional incubation apparatus for experiments is an experimental incubation apparatus capable of rapidly performing a culture test at a specific temperature on a sample to be tested for gene amplification culture or other test purposes.

However, as described above, the conventional incubation apparatus for experiments has a problem in that it is difficult to purchase and use in a small-scale laboratory because the purchase cost increases as the structure is complicated and the manufacturing cost increases.

Republic of Korea Utility Model Office 0127676

An object of the present invention, to solve these problems, is to provide a thermostat for an experiment vessel, which has a simple structure, can lower manufacturing costs, and can maintain a constant temperature of an experiment vessel accommodating cells and a culture medium. there is.

In order to achieve the above objects, a laboratory container thermostat according to an embodiment of the technical idea of the present invention includes a container part provided with a space therein to accommodate a laboratory container; a heating wire provided to surround the storage unit and electrically connected to a power supply unit to generate heat through power applied from the power supply unit and transfer heat to the storage unit; And a cover portion for closing the open portion of the upper portion of the container portion to prevent heat in the inner space of the container portion from being discharged to the outside; may include.

In addition, the cover part has a through-hole formed in the center portion through which the inlet for the experiment container passes, and a closing part for closing the open top of the container part; And it is formed to extend downward along the outer periphery of the closure portion is seated and fixed to the upper end of the container portion; may include.

In addition, in the closing part, a contact member made of a soft material is provided in a ring shape on the inner outer circumferential surface of the through hole, and the inner circumferential surface of the contact member is in close contact with the outer circumferential surface of the inlet of the experiment container of various shapes, so that the heat inside the container part Confidentiality can be maintained to prevent release.

In addition, the cover part is further formed with an extension part made of a material having high thermal conductivity so that one side of the closure part extends downward, and the heated air heated by the heat wire and accommodated in the inner space of the container part is supplied to the extension part. Heat is transferred, and by heating the extension part, it is possible to maintain the heat of the heated air accommodated in the inner space of the container part.

In addition, a plurality of container grooves capable of accommodating small experimental containers having a smaller size than the experimental containers accommodated in the container unit may be formed in the upper part of the cover part.

In addition, the cover unit may further include a container tray seated on the upper portion of the closure portion to transport a plurality of the small-sized laboratory containers at once.

In addition, in the cover part, a circular ring member made of a material having high thermal conductivity may be embedded inside the closure part.

In addition, the laboratory container thermostat may further include a supporting member disposed on an inner bottom of the container unit to support a lower portion of the laboratory container so that the laboratory container is spaced apart from the inner bottom of the container unit by a set distance.

In addition, the support member, the support portion supporting the lower portion of the vessel for the experiment; A leg portion having a plurality of legs installed below the supporting portion; and a support portion formed on an outer circumferential surface of the receiving portion to extend toward an upper oblique line and supported on an inner surface of the container portion.

In addition, the container unit is a container having a double structure having a space therein, and the hot wire may be embedded in the inner space of the container having the double structure.

In addition, the container unit is a double-structured container having a space therein, and a liquid, solid, or gaseous heat transfer medium is filled in the internal space, and the heat wire is installed to surround the outer circumferential surface of the double-structured container. It can be.

In addition, the container part is made of stainless steel, the inner diameter of the lower end is 8 cm, the inner diameter of the upper end is 6 cm, and the height is 8 cm. It may be made to accommodate an Erlenmeyer flask having a volume.

In addition, a temperature display unit coated with a Zion pigment that reacts at a set temperature and changes color may be installed in the container part so that the level of the heated temperature of the container part can be visually confirmed.

The laboratory vessel thermostat according to the present invention has a simple structure and can lower manufacturing cost, so it is easy to install and use. By keeping the constant, the appropriate temperature for the cell line can be maintained, and there is an effect of preventing cells from dying due to cold shock.

1 is an exploded perspective view of an experimental vessel thermostat according to an embodiment of the present invention.
Figure 2 is a cross-sectional view of the laboratory vessel thermostat of Figure 1;
3 is a cross-sectional view of a thermostat for a laboratory vessel according to another embodiment of the present invention, in which an adhesive member is provided on a closure portion.
4 is a cross-sectional view of a thermostat for an experimental vessel according to another embodiment of the present invention, in which a closing member and an extension part are provided.
5 is an exploded perspective view of a container thermostat for experiments according to another embodiment of the present invention in which a container groove and a container tray are provided in a cover portion.
FIG. 6 is a cross-sectional view of a thermostat for a lab vessel according to another embodiment of the present invention in which a ring member is attached to a cover of the thermostat for a lab vessel of FIG. 5;
7 is a cross-sectional view of an experimental vessel thermostat according to another embodiment of the present invention in which a supporting member is installed in the vessel unit.
8 to 9 are cross-sectional views of the laboratory vessel thermostat according to another embodiment of the present invention in which the vessel portion has a double structure.

In order to fully understand the present invention and its operational advantages and objectives achieved by the practice of the present invention, reference should be made to the accompanying drawings illustrating preferred embodiments of the present invention and the description in the drawings.

Hereinafter, the present invention will be described in detail by describing preferred embodiments of the present invention with reference to the accompanying drawings. Like reference numerals in each figure indicate like elements.

The laboratory vessel thermostat according to one embodiment of the technical idea of the present invention has a simple structure, thereby reducing manufacturing costs, and relates to a laboratory vessel thermostat capable of maintaining a constant temperature of the laboratory vessel accommodating cells and a culture medium. will be.

1 is an exploded perspective view of a thermostat for a laboratory vessel according to an embodiment of the present invention, and FIG. 2 is a cross-sectional view of the thermostat for a laboratory vessel of FIG. 1 .

Referring to FIGS. 1 and 2 , the laboratory vessel thermostat according to an embodiment of the present invention may largely include a container part 10 , a hot wire 20 and a cover part 30 .

First, the container unit 10 has a space provided therein to accommodate the laboratory container A1, where the laboratory container A1 may be a glass tube type container used in a typical laboratory, but is not limited to any one. In the description of the present invention, it will be described based on an Erlenmeyer flask.

The container part 10 may be formed to correspond to the shape of the container A1 for experiments. For example, as shown in the drawing, in the case of an Erlenmeyer flask, as the outer circumferential surface of the container is formed in a triangular cross section where the width decreases toward the upper side, the cross sectional shape of the container portion 10 corresponds to this and is basically a triangle. can be formed into shapes.

At this time, it is preferable that the inner diameter of the upper portion of the container part 10 is equal to or smaller than the lower diameter of the experimental container A1.

In addition, when the container A1 for experiment is accommodated in the inner space of the container part 10, a spaced space may be provided between the inner wall of the container part 10 and the outer surface of the container A1 for experiment.

Meanwhile, as an embodiment, the container part 10 is made of stainless steel, has an inner diameter of 8 cm at the lower end, an inner diameter of 6 cm at the upper end, and a height of 8 cm. (20) may be configured to be wound. This is made to accommodate an Erlenmeyer flask having a volume of 125 ml in the container part 10, and the above size standard of the container part 10 is the ratio of the size in proportion to the volume size of the Erlenmeyer flask. It can be made smaller or larger.

As such, the shape and size of the container unit 10 may be set to correspond to the size of the container A1 for experiments such as an Erlenmeyer flask, and the heat transmitted from the hot wire 20 is transferred to the container A1 for experiments. A constant relationship between the outer circumferential surface of the experimental vessel A1 and the inner circumferential surface of the container part 10 is prevented from transferring excessive heat to the experimental vessel A1 and the heat is evenly transferred throughout the experimental vessel A1 to maintain a uniform temperature. It may be made such that a gap is provided.

Next, the heat wire 20 is provided to surround the container part 10, and is electrically connected to a power supply device (not shown) to generate heat through power applied from the power supply device to generate heat. ) to transfer heat.

Here, the power supply device (not shown) may include all electrical devices such as a converter, a controller, a switch, and a plug for converting voltage by receiving power from the main power source.

Therefore, the hot wire 20 is a part that is electrically connected to such a power supply device, receives power, converts electrical energy into thermal energy, and emits heat.

The heat wire 20 is installed to wrap around the outer circumferential surface of the container part 10 at regular intervals, and may be formed in the form of a tube having a hollow cross section or in the form of a wire. At this time, in order to increase heat transfer efficiency, it is preferable to be made of a copper material having high thermal conductivity.

In this way, the heat wire 20 is installed around the outer circumferential surface of the container part 10 and when power is applied from the power supply device, it is heated and dissipates heat, thereby heating the outer circumferential surface of the container part 10, and the container part 10 ) As the outer circumferential surface is heated, heat is transferred to the inner space, and heat is transferred to the experimental vessel A1 accommodated in the inner space of the container unit 10, so that the set temperature can be maintained at all times.

Finally, the cover part 30 is to prevent the heat inside the container part 10 from being released to the outside so as to minimize heat loss and facilitate temperature maintenance. It is to close to prevent the heat of the inner space of the container part 10 from being discharged to the outside.

The cover part 30 has a through hole 311 through which the inlet of the experimental container A1 passes through the center portion, and a closing part 31 for closing the open top of the container part 10 and the It is formed to extend downward along the outer periphery of the closure part 31 and is seated and fixed to the upper end of the container part 10 (32); may include.

The closing portion 31 is a horizontal surface portion of the cover portion 30 , and the mounting and fixing portion 32 is a vertical portion vertically extending downward along the outer periphery of the closing portion 31 .

At this time, the seating and fixing part 32 may be formed such that a step is formed at an inner lower part so that it can be inserted into the upper entrance of the container part 10 and fixed. In addition, although not shown in the drawing, a screw thread may be optionally formed in place of the stepped portion of the mounting fixing part 32 instead of the stepped portion, in which case the container part 10 corresponds to the screw thread of the mounting fixing part 32. ) can be threaded at the inlet.

3 is a cross-sectional view of a thermostat for a laboratory vessel according to another embodiment of the present invention, in which a close-up member is provided at the closure part.

Referring to FIG. 3 , as another embodiment of the closure part 32 , a contact member 312 made of a soft material may be provided in a ring shape on an inner circumferential surface of the through hole 311 . At this time, the inner circumferential surface of the adhesion member 312 is in close contact with the outer circumferential surface of the inlet of various types of experiment containers, so that heat inside the container part 10 is not released to the outside, so that airtightness can be maintained.

For example, the contact member 312 is installed in a soft material having elasticity (eg, silicon, urethane, rubber, etc.) at the closing part 32 of the cover part 30, the cover part 30 When made of a release material, it can be stably installed on the inner circumferential surface of the closure part 32 through an insert injection method.

4 is a cross-sectional view of a thermostat for a laboratory vessel according to another embodiment of the present invention, in which a close-up member and an extension part are provided.

Referring to FIG. 4 , in another embodiment, the cover part 30 may further include an extension part 313 made of a material having high thermal conductivity so that one side of the closure part 31 extends downward.

The extension part 313 is connected to one side of the closure part 31 and may be disposed and extended in a space between the outer circumferential surface of the container A1 accommodated in the container part 10 and the inner surface of the container part 10. The extension part 31 may be a protruding form of a plurality of 'rods' or 'pins', or may be a cylindrical shape extending downward.

At this time, the heated air heated by the hot wire 20 and accommodated in the inner space of the container part 10 is transferred to the extension part 313, and the extension part 313 is heated so that the container part 10 It is possible to maintain the heat of the heated air accommodated in the inner space.

5 is an exploded perspective view of a container thermostat for experiments according to another embodiment of the present invention, in which container grooves and container trays are provided in the lid portion.

Referring to FIG. 5 , in another embodiment, the cover part 30 is a small experimental container A2 smaller in size than the experimental container A1 accommodated in the container part 10 above the closure part 31 . ) A plurality of container grooves 314 capable of accommodating may be formed.

Here, the small experimental container (A2) is a small container having a relatively small capacity compared to the experimental container (A1), and may be an 'EP tube' connected with a stopper.

Usually, after storing an object (e.g., cells) in a frozen state while accommodating it in a small experimental container (A2), when the experiment proceeds, it is thawed naturally at room temperature before the experiment. When thawing was performed, it took a lot of time to thaw, which made it difficult.

The present invention is to quickly defrost the small experimental container (A2) in order to solve these difficulties, and after inserting the small experimental container (A2) into the container groove 314, When operated and heated, heat is transferred to the cover part 30, so that the small experimental container A2 can be thawed in a short time.

At this time, the cover part 30 may further include a container tray 315 seated on the upper portion of the closure part 31 to transport a plurality of the small-sized experimental containers A2 at once.

Holes are perforated in the container tray 315 in a size and position corresponding to the container groove 314, and a step extending downward is formed on the outer periphery of the rim to be seated and fixed on the upper part of the lid part 30 It can be.

FIG. 6 is a cross-sectional view of the thermostat for experiment vessel according to another embodiment of the present invention in which a ring member is attached to the cover of the thermostat for experiment vessel of FIG. 5 .

Referring to FIG. 6 , as another embodiment of the cover part 30 , a circular ring member 316 made of a material having high thermal conductivity may be embedded inside the closure part 31 .

The ring member 316 is to increase the heat transfer efficiency of the small experimental vessel A2 accommodated in the vessel groove 314, and is embedded in an inner portion adjacent to the vessel groove 314 to transfer heat from the heat wire 20. It may be made of a material with high thermal conductivity to absorb and release heat more easily.

7 is a cross-sectional view of an experimental vessel thermostat according to another embodiment of the present invention in which a support member is installed on the vessel part.

Referring to FIG. 7 , in another embodiment, the thermostat for the experiment vessel is disposed on the inner bottom of the vessel part 10 to support the lower part of the vessel A1 for the experiment, so that the vessel A1 for the experiment is the container part ( 10) A support member 40 spaced apart from the inner floor by a set distance may be further included.

The supporting member 40 includes a supporting portion 41 supporting the lower portion of the experimental container A1, a leg portion 42 having a plurality of legs installed below the supporting portion 41, and an upper portion of the outer circumferential surface of the supporting portion 41 It may include a support part 43 formed to extend obliquely and supported on an inner surface of the container part 10 .

That is, as shown in the drawing, the supporting member 40 is to keep the experimental vessel A1 apart from the bottom of the container part 10, and the supporting member 40 is easily disposed at the center position. The support portion 43 may be extended to the outer periphery of the rim of the support portion 41 so as to be stably fixed without being separated, and may be installed to be supported on the inner surface of the container portion 10.

At this time, the supporting portion 41 may be formed to have a mesh structure or a plurality of holes passing therethrough.

Through this, it is possible to maintain a constant temperature as the heat transfer to the bottom portion of the experimental vessel A1 is easy and uniform heat transfer is possible to the entirety of the experimental vessel A1.

8 to 9 are cross-sectional views of the laboratory vessel thermostat according to another embodiment of the present invention in which the vessel part has a double structure.

Referring to FIG. 8 , as another embodiment, the container part 10 'is made of a container having a double structure having a space therein, and the hot wire 20 may be embedded in the inner space of the container of the double structure. there is.

That is, the heat wire 20 is not exposed to the outside and is embedded inside the double-structured container to improve aesthetic effects, and as the heat wire 20 is embedded in the inner space of the container, heat loss is minimized to increase thermal efficiency. You can get as many effects as possible.

Referring to FIG. 9, as another embodiment, the container part 10' is a container having a double structure having a space therein, and a heat transfer medium of any one of liquid, solid, and gas is filled in the inner space, and the heat wire (200 may be installed to surround the outer circumferential surface of the container of the double structure.

In this way, since the heat transfer medium is accommodated in the inner space of the container part 10', heat loss is prevented for a certain period of time after the container part 10' is heated by the heating wire 20, and the effect of maintaining a certain temperature for a long time is achieved. be able to get

On the other hand, in the case of the double-structured container, which is not shown in the drawings, the heat wire may be installed in the container part 10' in a state where the heat transfer medium is accommodated so as to be embedded in the space inside the container part 10'.

On the other hand, although not shown in the drawings, the container parts 10 and 10' change color in response to a set temperature so that the user can visually check the degree of heated temperature of the container parts 10 and 10'. A temperature display unit (not shown) coated with Zion pigment may be installed.

This cyan pigment is a pigment whose color changes according to temperature, and has a characteristic of changing color when the reference temperature is reached. The user can easily check the temperature of the parts 10 and 10' with the naked eye.

For example, the configuration of the temperature display unit can be completed by simply applying a cyan pigment of a specific color to the outer surfaces of the container units 10 and 10'. The cyan pigment applied to the outer surface of the container portion (10, 10') initially has a specific color and is visually confirmed. When the reference temperature is reached, the color disappears, and when the temperature is lowered again than the reference temperature, the original color is restored. will come back Through this, the fact that a cyan pigment color of a specific color is shown on the temperature display part of the container parts 10 and 10' means that the reference temperature is not reached, and in this case, the heating temperature of the heating wire 20 can be further increased. It means there is a need.

That is, through the temperature display unit, the user can easily check the temperature required for the experiment with the naked eye, and thus the temperature maintenance is facilitated.

The laboratory vessel thermostat according to various embodiments according to the technical idea of the present invention has a simple structure and can reduce manufacturing cost, so it is easy to install and use, can be used in a small laboratory without burden, and can be used without burden on cells and culture medium. By keeping the temperature of various types of experimental containers that are accommodated at a constant level, it is possible to maintain an appropriate temperature suitable for the cell line, thereby preventing cells from dying due to cold shock.

As described above, the optimum embodiment has been disclosed in the drawings and specifications. Although specific terms are used herein, they are only used for the purpose of describing the present invention and are not used to limit the meaning or the scope of the present invention described in the claims. Therefore, those of ordinary skill in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

10, 10': container part 20: hot wire
30: cover part 31: closing part
311: through hole 312: adhesion member
313: extension part 314: container groove
315: container tray 316: ring member
32: fixing part 40: support member
41: supporting part 42: leg part
43: support A1: vessel for experiment
A2: Small laboratory vessel

Claims (13)

A container unit provided with a space therein to accommodate a container for an experiment;
a heating wire provided to surround the storage unit and electrically connected to a power supply unit to generate heat through power applied from the power supply unit and transfer heat to the storage unit; and
and a cover part for closing the open portion of the upper part of the container part to prevent heat from being discharged to the outside of the inner space of the container part. According to claim 1,
The cover part,
a closing part having a through hole through which an entrance of an experiment container passes through the central part and closing the open upper part of the container part; and
and a mounting and fixing portion formed to extend downward along the outer circumference of the closure and seated and fixed to an upper end of the container. According to claim 2,
the closure,
A contact member made of a soft material is provided in a ring shape on the inner outer circumferential surface of the through hole,
The inner circumferential surface of the adhesion member is in close contact with the outer circumferential surface of the inlet of the experimental vessel of various shapes to maintain airtightness so that heat inside the container unit is not released. According to claim 3,
The cover part,
An extension portion made of a material having high thermal conductivity is further formed so that one side of the closure portion extends downward,
The heated air heated by the heating wire and accommodated in the inner space of the container part is transferred to the extension part, and the extension part is heated to maintain the heat of the heated air accommodated in the inner space of the container part. Laboratory vessel thermostat. According to claim 2,
The cover part,
A laboratory container thermostat, characterized in that a plurality of container grooves capable of accommodating small-sized laboratory containers smaller in size than the laboratory containers accommodated in the container part are formed in the upper part of the closure part. According to claim 5,
The cover part,
The laboratory container thermostat further comprising a container tray seated on an upper portion of the closure and transporting a plurality of the small-sized laboratory containers at once. According to claim 5,
The cover part,
Experiment vessel thermostat, characterized in that a circular ring member made of a material with high thermal conductivity is built inside the closure. According to claim 1,
The laboratory vessel thermostat,
The laboratory vessel thermostat further comprising a support member disposed on an inner bottom of the container unit to support a lower portion of the experiment vessel so that the experiment vessel is spaced apart from the inner bottom of the vessel unit by a set distance. According to claim 8,
The supporting member is
a supporting portion supporting a lower portion of the experimental container;
A leg portion having a plurality of legs installed below the supporting portion; and
and a support part formed on an outer circumferential surface of the support part to extend toward an upper oblique line and supported on an inner surface of the container part. According to claim 1,
The container part,
It is a double-structured container with a space inside,
The laboratory vessel thermostat, characterized in that the hot wire is built into the inner space of the vessel of the double structure. According to claim 1,
The container unit is a double structure container having a space therein, and a heat transfer medium of any one of liquid, solid or gas is filled in the internal space,
The laboratory container thermostat, characterized in that the heating wire is installed to surround the outer circumferential surface of the double-structured container. According to claim 1,
The container part,
Made of stainless steel, the inner diameter of the lower part is 8 cm, the inner diameter of the upper part is 6 cm, and the height is 8 cm.
It is configured so that the heat wire having a length of 1 m is wound on the container part,
Experimental container thermostat, characterized in that configured to accommodate an Erlenmeyer flask having a volume of 125 ml in the container part. According to claim 1,
In the container part,
A temperature display unit coated with a Zion pigment that changes color in response to a set temperature is installed so that the degree of heated temperature of the container unit can be visually confirmed.

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