KR20200121828A - Anti-pollution agent and composition for external skin - Google Patents

Abstract

It provides an anti-pollution agent and an external skin composition for anti-pollution excellent in preventing or inhibiting skin inflammation caused by particulate air pollutants. An anti-pollution agent in which the plant-derived polyamine-containing extract prevents or suppresses skin inflammation caused by air pollutants.

Description

Anti-pollution agent and composition for external skin

The present invention relates to an anti-pollution agent for alleviating damage to the skin by air pollutants, in particular particulate air pollutants, and a composition for external use for skin containing the anti-pollution agent.

The skin is the largest tissue in the outermost layer and is exposed to external stimuli such as ultraviolet rays, microbes, viruses and air pollutants. It has been studied for a long time that UV rays cause skin aging by damaging the skin. On the other hand, in recent years, an increasing number of examples have been reported about the possibility that the adhesion of particulate air pollutants to the skin is related to aging (Non-Patent Document 1). In addition, benzopyrene contained in tobacco activates the aryl hydrocarbon receptor present in the epidermal cells, prompting the induction of cytochrome P450 (CYP1), a drug metabolizing enzyme, and the production of active oxygen. , It has been reported that the production of interleukin 8 is induced to induce an inflammatory response (Non-Patent Document 2).

In order to protect the skin from these external stimuli, do not adhere or penetrate substances that may cause damage such as particulate air pollutants to the skin and/or mitigate the effects of the attached irritating substances on the skin. Things to do are considered.

As described above, as a measure to protect the skin, conventionally, there is a proposal of a formulation that forms a film on the skin so that particulate air pollutants do not adhere to the skin or can be easily washed away even if it adheres. Because the coating was coated with a film, the feeling in use was poor, and it was unclear whether or not it was effective against all irritating substances.

It has been studied that particulate air pollutants attach and infiltrate the skin to impart oxidative stress to cells, causing inflammatory reactions, cell metabolism, and gene damage, but the mechanisms of cell damage include oxidation, inflammation, and cell metabolism. It is a reality that there are not so many materials that have antipollution effects in the market because there are many unknown parts such as abnormalities in gene expression.

For example, it was found that the extract of the genus Hippopii genus Bodhisattva suppresses the rise of PGE2 caused by atmospheric aerosol particles such as particulate air pollutants, thereby inhibiting skin inflammation caused by atmospheric aerosol particles (Patent Document 1). . In addition, paying attention to the mechanism by which contaminants infiltrating the skin induce skin inflammation in epidermal cells, the effect of mitigating cytotoxicity due to zinc salts and/or zinc complexes of amino acids, CYP1 excess induction, COX2 excess induction, It has found usefulness such as inhibition of the IL8 excessive induction action (Patent Document 2).

In addition to the above circumstances, the viewpoint of diversification of formulations and expansion of options for prescription formulation, the viewpoint of synergistic effect using anti-pollution agents against particulate air pollutants with different mechanisms, It goes without saying that the development of a new anti-pollution agent is required from the viewpoint of a pollutant agent.

Polyamine is a generic term for aliphatic hydrocarbons having two or more primary amino groups, and is a natural product that is universally present in the living body, and more than 20 types of polyamines have been found. Representative polyamines include putrescine, spermidine, and spermine. The main physiological actions of polyamines are (1) stabilization and structural changes of nucleic acids by interaction with nucleic acids, (2) promoting effects on various nucleic acid synthesis systems, (3) activation of protein synthesis systems, and (4) stabilization of cell membranes. Although enhancement of membrane permeability of substances, (5) elimination of active oxygen, and (6) promotion of cell proliferation are known, the effect of preventing or suppressing skin inflammation caused by particulate air pollutants has not been confirmed so far.

Japanese Patent Publication No. 2016-216366 Japanese Patent Publication No. 2016-88929

J Eur Acad Dermatol Venereol. 2015 Dec; 29(12): 2326-32 J Dermatol Sci. 2011 Apr; 62(1): 42-9

The present invention provides an anti-pollution agent excellent in preventing or inhibiting skin inflammation caused by particulate air pollutants, and an external composition for skin using the anti-pollution agent.

The present inventors have found that the plant-derived polyamine-containing extract has an anti-pollution effect that relieves skin inflammation caused by air pollutants, and has completed the present invention.

That is, the outline of the present invention is as follows.

Item 1. Anti-pollution agent containing a plant-derived polyamine-containing extract as an active ingredient.

Item 2. The plant-derived polyamine-containing extract is an extract derived from at least one material selected from the group consisting of soybean seeds, soybean embryos, soybean pears, soybeans, wheat seeds, wheat embryos, wheat pears, wheat malt, soy milk, and bean fat. The anti-pollution agent according to item 1.

Item 3. The antipollution agent according to item 1 or 2, wherein the plant-derived polyamine-containing extract is an extract derived from a soybean embryo.

Item 4. The antipollution agent according to any one of Items 1 to 3, which contains at least one or more compounds selected from the group consisting of putrescine, spermidine and spermine as polyamine.

Item 5. Antipol according to any one of items 1 to 4 having an action of inhibiting the expression of at least one gene selected from the group consisting of interleukin-1α (IL1α), interleukin-8 (IL8) and cytochrome P450 (CYP1). Lusion system.

Item 6. A composition for external use for skin containing the anti-pollution agent according to any one of items 1 to 5.

By using the plant-derived polyamine-containing extract of the present invention, prevention or suppression of skin inflammation caused by particulate air pollutants can be expected, and an external preparation for skin for the purpose of this effect can be provided.

1 is a result of the expression level of IL1α after 2 hours after treatment with urban air dust (environmental standard substance/CRM No.28) after 5 hours after performing soybean extract treatment on keratinocytes in advance in Example 1 It is a figure showing.
Fig. 2 is a result of the expression level of IL8 after 2 hours after treatment with soybean extract on keratinocytes in Example 1, treatment with urban air dust (environmental standard substance/CRM No.28) after 5 hours It is a figure showing.
3 is a result of the expression level of CYP1 after 2 hours after treatment with urban air dust (environmental standard substance/CRM No.28) after 5 hours after performing soybean extract treatment on keratinocytes in advance in Example 1 It is a figure showing.

In the present invention, the air pollutant refers to a toxic substance or a group of toxic substances existing in the atmosphere. Specific examples include soot, dust, automobile exhaust gases, specific substances, harmful air pollutants and volatile organic compounds (VOC), which are regulated by the Air Pollution Prevention Act (independent administrative agency's website ; https://www.erca.go.jp/yobou/taiki/taisaku/01_01.html), but not limited to these. More specifically, a suspended particulate matter including a plurality of harmful substances such as PM10 and PM2.5 may be mentioned, and the anti-pollution agent of the present invention and the external composition for skin including the anti-pollution agent include PM2 It can prevent or suppress skin inflammation caused by .5. In the present invention, anti-pollution refers to an effect of preventing or suppressing the inflammatory reaction of the skin caused by air pollutants. Specifically, it is also possible as a criterion for judgment to exhibit an effect of suppressing gene expression of IL1α, IL8 or CYP1, but is not particularly limited thereto.

In the present invention, the "plant extract" is obtained from a plant and/or a plant processed product.

In the present invention, the term "plant-derived polyamine-containing extract" refers to a plant extract containing a (natural) polyamine obtained from a plant and/or a plant processed product, three types of (natural) putrescine obtained from a plant and/or a plant processed product, It is a plant extract containing fermidine, a plant extract containing spermine, and three types of (natural) putrescine, spermidine and spermine obtained from plants and/or plant processed products. In the present invention, "plant-derived polyamine-containing extract" may be referred to as "plant extract containing polyamine and/or plant extract containing polyamine as an active ingredient". The polyamine concentration contained in the plant-derived polyamine-containing extract is usually 0.00001 to 100 mM, preferably 0.00005 to 75 mM, and more preferably 0.0001 to 50 mM in M (mole/liter). In addition, the weight% is usually 0.0001 to 100%, preferably 0.001 to 75%, and more preferably 0.01 to 50%.

Since the plant-derived polyamine-containing extract simultaneously contains natural active ingredients other than putrescine, spermidine, and spermine polyamine, a composition containing chemically synthesized putresin, spermidine, and spermine individually It is estimated that the anti-pollution effect will be superior. Examples of natural active ingredients include sugars such as monosaccharides and oligosaccharides, peptides, and proteins. In addition, since the plant-derived polyamine-containing extract is an aqueous solution, the production conditions are mild compared to individual polyamine compounds, and a high advantage in terms of safety is expected.

In the present invention, polyamine is a generic term for aliphatic hydrocarbons having two or more primary amino groups, and is a natural product universally present in a living body, and 20 or more types of polyamines have been discovered. For example, 1,3-diaminopropane, putrescine, cadaverine, caldine, spermidine, homospermidine, aminopropyl cadaverine, thermine, spermine, thermosfermine, cannabalmin, And aminopentylnorspermidine, N,N-bis(aminopropyl)cadaverine, homospermine, caldopentamine, homocaldopentamine, caldohexamine, homocaldohexamine, and the like. Representative polyamines include putrescine, spermidine, and spermine.

"Putresin" as referred to in the present invention is one of the representative polyamines and is a general natural product that is universally present in living organisms, and is an aliphatic hydrocarbon compound having two primary amino groups. "Spermidine" is one of the representative polyamines, and is a general natural product that is commonly present in living organisms, and is an aliphatic hydrocarbon compound having three primary amino groups. "Spermine" is one of the representative polyamines and is a general natural product that is universally present in living organisms, and is an aliphatic hydrocarbon compound having four primary amino groups.

Plant extracts containing polyamines and/or plants for obtaining plant extracts containing polyamines as active ingredients are not particularly limited, but for example, dicotyledons, monocotyledons, herbaceous plants, woody plants, cucurbita, and alanaceae Plants, rice plants, cress, legumes, mallow family plants, asteraceae plants, quinceaceae plants, legumes, plant extracts, and plant extracts. For example, sweet potato, tomato, cucumber, pumpkin, melon, watermelon, tobacco, Arabidopsis, bell pepper, eggplant, bean, taro, spinach, carrot, strawberry, potato, rice, corn, alfalfa, wheat, barley, soybean, rapeseed Seed, sorghum, eucalyptus, poplar, kenaf, headworm, sugarcane, sugar beet, cassava, sago palm, pollock, lily, orchid, carnation, rose, chrysanthemum, petunia, torenia, snapdragon, cyclamen, gypsophila, geranium, sunflower, Grass, cotton, enoki mushroom, pulp mushroom, pine mushroom, shiitake, mushrooms, Korean ginseng, agaricus mushroom, turmeric, ginseng, citrus, green tea, black tea, oolong tea, banana, kiwi, natto, soy milk, soybean extract, Wheat extract, embryo extract, pear extract, fruit juice, bean jam, rice embryo, wheat embryo, barley embryo, soybean embryo, corn embryo, milo embryo, sunflower embryo, and the like.

Preferably, monocotyledons or dicotyledons are good, more preferably rice plants or legumes are good, and particularly preferable plants or processed products thereof include corn, mushrooms, soybeans, wheat, natto, soy milk, bean curd, wheat germ, soybean germ , Corn embryo, soybean extract, wheat extract, embryo extract, and pear extract. More preferably, a soybean seed, a soybean embryo, a soybean pear, a soybean seed, a wheat seed, a wheat embryo, a wheat pear, a wheat germ, a soybean milk, and a biji are mentioned.

The plant extract containing polyamine and/or the plant tissue for obtaining the plant extract containing polyamine as an active ingredient is not particularly limited. It is preferably in the form of seeds and growth. Plants in the process of growth can be obtained from whole or partial tissues. The obtained site is not particularly limited, but the whole tree, flowers, buds, ovary, fruits, leaves, cotyledons, stems, shoots, roots, seeds, dried seeds, pears, embryos, roots, and the like. Preferably, they are fruits, leaves, stems, shoots, seeds, dried seeds, embryos, and pears, and particularly preferably seeds, dried seeds, embryos, and pears.

Plant extracts containing polyamines and/or plant extracts containing polyamines as an active ingredient may be processed plants. Its processing method uses water, an organic solvent, a mixture of water and an organic solvent, and the like, and impregnation method under low temperature, room temperature, and heating conditions, distillation method, compression method, ultrasonic method, supercritical fluid method, subcritical fluid method, etc. Recover the extract. Also included are processed products such as fermenting plants or extracts recovered from plants. For example, plant extract, soy milk, bean curd, wheat powder, fermented extract, natto, etc. are mentioned.

As a method of obtaining a plant extract containing polyamine and/or a plant extract containing polyamine as an active ingredient, there is a method of adding an acid solution to the plant and/or plant processed product so as to be under acidic conditions. The acidic condition refers to a condition in which the pH is 6 or less. By setting the pH under acidic conditions at the time of extraction, the effect of efficient and stable recovery of the polyamine composition from plant tissues is obtained. This effect is uniformly obtained when the pH is 6 or less, but preferably the pH is 4 or less, particularly preferably the pH is 2 or less. The lower limit is not particularly limited because it does not matter the pH of the stock solution of the acid solution to be used, but is preferably pH 0 to 2.

By obtaining the plant extract under acidic conditions (aqueous acid solution), the recovery rate of the amount of polyamine is higher than that of the plant extract recovered with an organic solvent such as ethanol or methanol. In particular, compounded polyamines, which are difficult to dissolve in water, are not dissolved in water due to acidic conditions. Since it can be solubilized, the content of free polyamines having low extraction efficiency in organic solvents is also improved. Polyamines exhibit excellent stability under acidic conditions, and the stability of polyamines and other active ingredients contained in plant extracts is improved. Further, by obtaining a plant extract under acidic conditions (an aqueous acid solution), natural active ingredients other than polyamines are simultaneously recovered. Examples of natural active ingredients include sugars such as monosaccharides and oligosaccharides, peptides, and proteins. The anti-pollution effect is further enhanced by simultaneously including polyamine and natural active ingredients other than polyamine. Moreover, since the recovered plant extract is an aqueous solution, it is expected to have a high advantage in terms of safety compared to organic solvents.

Examples of acid solutions added under acidic conditions include inorganic acids such as hydrochloric acid, sulfuric acid, and phosphoric acid, acetic acid, propionic acid, butyric acid, oxalic acid, malonic acid, succinic acid, fumaric acid, maleic acid, malic acid, lactic acid, tartaric acid, citric acid, benzoic acid, and other organic acids. And acidic water, but inorganic acids and organic acids such as 0.01N to 6N hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trichloroacetic acid, sulfosalicylic acid, formic acid, citric acid, lactic acid, and 0.1 to 10% perchloric acid.

Plant extract containing polyamine by adding an acid solution to a plant and/or a plant product under acidic conditions or adding an acid solution to an acidic condition and then adding a polyphenol adsorbent and then crushing, pulverizing, and mixing And/or it is possible to increase the recovery amount of the plant extract containing the polyamine as an active ingredient. In particular, in the case of plant tissue, it is preferable to damage the cell wall in terms of having a cell wall. In the case of plant processed products or plant extracts, since the cell wall is not included, there is no need to perform crushing or pulverization that damages the cell wall in particular. As a method of crushing or pulverizing, for example, a mixer, a blender, a homogenizer, a mortar, an ultrasonic crusher, or the like can be used.

After the polyamine contained in the plant and/or plant product is sufficiently extracted in an acid solution (liquid fraction), the liquid fraction is separated from the residue or precipitate by centrifugation or filtration separation. The recovered liquid fraction contains many polyamines, and "a plant extract containing polyamine and/or a plant extract containing polyamine as an active ingredient" can be obtained.

The plant extract containing polyamine and/or the plant extract containing polyamine as an active ingredient may be used as it is as an anti-pollution agent, but it is preferably used in combination as an external preparation for skin. The concentration of the polyamine is determined by the degree of absorption, degree of action, product form, frequency of use, etc., and is not particularly limited, but is usually 0.00001 to 100 mM, preferably 0.00005 to 75 mM, and more preferably 0.0001 to 50 mM.

The anti-pollution agent and the composition for external skin for anti-pollution of the present invention contain a plant-derived polyamine-containing extract as an active ingredient. In the present invention, anti-pollution refers to an effect of alleviating skin damage caused by air pollutants. In more detail, damage to the skin can be alleviated by preventing or suppressing inflammation of cells, for example, caused by adhesion or penetration of air pollutants to the skin.

The basis of the action of the anti-pollution effect of the present invention is that the plant-derived polyamine-containing extract, which is an active ingredient of the present invention, acts inside and outside the epidermal cells of the skin, and the inflammatory action of the cells expressed by adhesion or invasion of air pollutants It is speculated that it is easing.

More specifically, it is thought that the anti-pollution effect in the present invention is due to suppression of gene expression of IL1α and IL8, which are inflammatory mediators in cells induced by particulate air pollutants. Furthermore, it is thought to be due to suppressing the gene expression of the drug metabolizing enzyme CYP1 involved in inducing the expression of these inflammatory mediators.

In addition to the plant-derived polyamine-containing extract, which is an active ingredient, the anti-pollution agent and the composition for external use of the skin of the present invention may contain other raw materials within a range not impairing the effects of the present invention. Examples of such raw materials include water, excipients, antioxidants, preservatives, wetting agents, viscous agents, buffers, adsorbents, solvents, emulsifiers, stabilizers, surfactants, lubricants, water-soluble polymers, sweeteners, flavoring agents, acidulants, alcohols, etc. have. In addition, the anti-pollution agent and the composition for external use of the skin of the present invention may contain other active ingredients within a range that does not impair the effects of the present invention. As a specific example of the active ingredient, for example, an antioxidant component, an anti-aging component, an anti-inflammatory component, a whitening component, a cell activation component, vitamins, a blood circulation promoting component, a moisturizing component, a DNA damage prevention and/or repair action. Components, anti-glycosylating components, peptides or derivatives thereof, amino acids or derivatives thereof, hydroquinone glycosides and esters thereof, and the like.

In addition to the essential components of the plant-derived polyamine-containing extract, the anti-pollution agent of the present invention is a component used in cosmetics, quasi-drugs, foods, pharmaceuticals, etc., as long as the effect of the present invention is not impaired if necessary. Additives can be used in combination and blended.

For example, as oils and fats, avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil, orange rape oil, sesame oil, cacao butter, chamomile oil, carrot oil, cucumber oil, tallow, tallow fatty acid, kukui nut oil , Safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, almond oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cacao butter, palm oil, palm kernel oil, wood wax, palm oil, tallow, pork oil, hydrogenated oil And hydrogenated castor oil.

Examples of the waxes include beeswax, carnauba wax, sperm wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, and the like.

As mineral oil, liquid paraffin, petrolatum, paraffin, ozokerite, ceresin, microcrystalline wax, polyethylene powder, squalene, squalane, pristane, etc. are mentioned.

Fatty acids include natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, Synthetic fatty acids, such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid, are mentioned.

As alcohols, natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol Synthetic alcohols such as ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide , Propylene glycol, polypropylene glycol, 1,3-butylene glycol, glycerin, batyl alcohol, pentaerythritol, sorbitol, mannitol, glucose, and polyhydric alcohols such as sucrose.

Examples of esters include isopropyl myristic acid, isopropyl palmitate, butyl stearate, hexyl laurate, myristic myristic acid, oleyl oleate, decyl oleate, octyldodecyl myristic acid, hexyldecyl dimethyloctanoate, Cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

Examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristic acid, zinc laurate, zinc undecylenate, and the like.

Examples of gums and water-soluble high molecular compounds include gum arabic, benzoin, damper rubber, guarine, irish moss, karaya rubber, tragacanth rubber, carob rubber, succinate seeds, agar, casein, dextrin, gelatin, pectin, starch , Carrageenan, carboxyalkylchitin, chitosan, hydroxyalkylchitin, low-molecular chitosan, chitoate, chitin sulfate, chitin phosphorylated, alginic acid and salts thereof, hyaluronic acid and salts thereof, chondroitin sulfate, heparin, ethylcellulose, methylcellulose, carboxy Methylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, polyvinyl methacrylate, poly Acrylate, polyalkylene oxide such as polyethylene oxide or polypropylene oxide, or crosslinked polymers thereof, carboxyvinyl polymer, polyethyleneimine, and the like.

As surfactants, anionic surfactants (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant (carboxylic acid type amphoteric surfactant, sulfuric acid ester type amphoteric) Surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant) Active agents, nitrogen-containing nonionic surfactants), and other surfactants (natural surfactants, derivatives of protein hydrolysates, polymeric surfactants, surfactants containing titanium and silicon, and fluorocarbon surfactants), etc. .

Vitamins include retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A) in vitamin A group, thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin in vitamin B group (Vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline, inositol, ascorbic acid and its derivatives in the vitamin C group, and vitamin D in the vitamin D group Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, tocopherol and its derivatives in the vitamin E group, ubiquinones, phytonadione (vitamin K1) in the vitamin K group, menaquinone (Vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), etc. are mentioned.

As amino acids, valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine , Ornithine, histidine, and the like, their sulfates, phosphates, nitrates, citrates, or amino acid derivatives such as pyrrolidonecarboxylic acid.

As a whitening agent, ascorbic acid or a derivative thereof, sulfur, placental hydrolyzate, ellagic acid or a derivative thereof, kojic acid or a derivative thereof, glucosamine or a derivative thereof, arbutin or a derivative thereof, hydroxycinnamic acid or a derivative thereof, glutathione, arnica extract, gold Extract, Sangbaek extract, Shiho extract, windbreak extract, Reishi mushroom mycelium culture or its extract, basswood extract, peach leaf extract, Youngsil extract, Gosam extract, Jiyu extract, Angelicae extract, Uiiin extract, Sweet persimmon leaf extract, Rhubarb extract, Mokdanpi extract, hamamelis extract, maronier extract, red pepper sprout extract, oil-soluble licorice extract, etc. are mentioned.

As a moisturizer, hyaluronic acid, polyglutamic acid, serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid, glyceroglycolipid, sphingophospholipid, Sphingolipids, linoleic acid or esters thereof, eicosapentaenoic acid or esters thereof, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, bitter mycelium culture or extract thereof, wheat germ oil, avocado oil, rice Germ oil, jojoba oil, soybean phospholipids, γ-orizanol, marshmallow extract, uiyiin extract, rhubarb extract, jujube extract, seaweed extract, cran's aloe extract, burdock extract, rosemary extract, arnica extract, wheat bran, and the like.

As a hair restorer, pentadecanoic acid glyceride, choleus extract, gentiana extract, pine cone extract, royal jelly extract, sackcloth extract, t-flavanone, 6-benzylaminopurine, sugar extract, carpronium chloride, minoxidil, finasteride, Adenosine, nicotinic acid amide, mulberry root extract, rhubarb extract, 5-aminolevulinic acid, and the like.

As extracts or extracts of animals, plants, or herbal medicines, Asun-Yak, Acerola, Altea, Arnica, Avocado, Persimmon Tea, Aloe, Aloe Vera, Nettle, Ginkgo Biloba (Ginkgo Biloba, Ginkgo Biloba), Fennel, Turmeric, Pedigree ( Sesin), Plum (Omae), Prickly Pear Tree, Uba Ursi, Brier Flower (Yeongsil), Banga Grass (Yeonmyeongcho), Astragalus, Golden, Wild Cherry Tree (Cherry Blossom), Hwangbyeok Tree (Hwangbaek), Yellow Lotus, Ginseng, Red Pepper Sprouts (Jejejeolcho), cheek beard, watercress, orange, raw paper, lamiaceae (hagwort), hassuo, pica tree (gumhwa), mugwort, bongsul (ahchul), arrowroot (brown root), valerian (valley grass), chamomile, Haneul Tari (Great Root), Four Season Mugwort (Injin Mugwort), Licorice, Butterbur (Kanto Flower, Kanto Leaf), Raspberry, Kiwi Fruit, Bellflower (Plant), Chrysanthemum, Gae Odong (Fruit), Citrus Fruit (Kisil), Tangerine (Tangerine) Tree bark), cucumbers, stalks or wild flowers (ganghwal, doldrums), apricots (passenger), goji chinensis (jigolpi, wolfberry, goji leaves), gosam, camphor tree, scallop, grapefruit fruit, cinnamon, hyeonggae, cassiae tea (gyeolmyeongja), Round leaf morning glory or morning glory (Gyeonwooja), safflower (safflower), Obaeja, Comfrey, Copaiba, Gardenia (Sanchija), Gentiana, Humpback (Hubbaek), iron knee, Osuyu, Burdock, Schisandra, rice, rice bran, wheat, Hyeopjeopsiho (shiho), saffron, saponaria, hawthorn tree (mountain lion), sancho tree, sage, samchil ginseng, shiitake mushrooms, rhubarb, sagunja, japonica, perilla leaf, perilla leaf, persimmon, peony, plantain ( Chajeon, Chajeoncho), Ginger, Iris, Yeojeongja, Hayacho, Birch, Honeysuckle (Gold and Silver), Yarrow, Yarrow, Western Alley, Red Beans (Red Soybean), Deotsana (Spirit Alley), Dang Awook, Cheongung, Dang-Yak, Mulberry (Sangbaekpi, Upper Leaf), Jujube, Soybean, Aralia, Bamboo Pickled Ginseng, Jimo, Cucumber Plant (Jiyu), Yakmo-Mil (Sip-Yak), Cordyceps, Red Pepper, Chrysanthemum (Deungri), Silver-Ban Thyme, Green Tea, Black Tea, Clove tree, Onju citrus persimmon (dermis), Camellia, Centella asiatica, red pepper, Angelica, marigold, Gwangtangerine (London), Cucumber plant (Lee oil), Corn (Nammanmo), Duchung (Leodworm, Duchungleaf), Tomato, Namcheon (Namcheonsil ), garlic, barley (malt), ringworm (baekseonpi), lobule vein mungong (maekmundong), parsley, batata, Peppermint, hamamelis, rose, loquat leaves, bokryeong, grapes or their leaves, sorghum cucumber, bodhi tree, mokdan (mokdan skin), hops, barberry (barberry), pine leaves, maronier, rosemary, no-patient tree, melissa, chinensis, quince , Bean sprouts, peaches (peach, peach leaves), beom fan (sagan), betel palm (beech kernel), motherwort, cornflower, rock-chuck (hoicho), hornwort tree (yangmaepi), four-tailed duck (real tea), adlay (eui-in) ), Mugwort, Wild Mugwort, Lavender, Apple Fruit, Ganoderma lucidum mushroom, Lemon fruit, Yeongyo, Prunus chinensis, Shigella (Nogwancho), Maniac grass (Scofolia root), Chicken rice sprout, Placenta extract of cattle and humans, stomach of pigs and cattle, Duodenum or intestinal extract or its decomposition product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, silk protein, silk protein decomposition product, bovine blood cell protein decomposition product, and the like.

Examples of microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, euglena extract, and lactic acid fermentation products of skim milk powder.

Examples of the α-hydroxy acid include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

Inorganic pigments include anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, iron sulfate, bengala, black iron oxide, Ultramarine, chromium oxide, chromium hydroxide, carbon black, calamine, and the like.

As ultraviolet absorbers, p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, campo derivatives, furan derivatives , Pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaazulene, sikonin, baicalin, baicalein, berberine, and the like.

Examples of astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenol sulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid, and the like.

Antioxidants include ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaiaretic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), and parahydroxyanisole. , Propyl gallate, sesamol, sesamolin, and gosipol.

Anti-inflammatory agents include iktamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-campo, hydrocortisone, guaiazulen, camazulene, chlorpheniramine maleate, glycyrrhizin. Acids and salts thereof, glycyrrhetinic acid and salts thereof, and the like.

Examples of the sterilizing and disinfecting agents include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methyllosanillin chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulpamine, mercurochrome, lactoferrin, or a hydrolyzate thereof.

Hair solvents include selenium disulfide, alkyl isoquinolinium bromide, zinc pyrithione, biphenamine, thiantol, castali tincture, ginger tincture, red pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate, thioglycol And mountains.

As fragrances, natural animal fragrances such as musk, civit, castorium, and amber lees, anise essential oil, angelica essential oil, ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, Kananga essential oil, caraway essential oil, cardamom essential oil, guaiacwood essential oil , Cumin Oil, Ginger Tree Oil, Shinnam Oil, Cinnamon Oil, Geranium Oil, Copaiba Balsam Oil, Coriander Oil, Perilla Oil, Cedarwood Oil, Citronella Oil, Jasmine Oil, Ginger Grass Oil, Cedar Oil, Spearmint Oil , Western peppermint essential oil, Daejanghyang essential oil, Wolhahyang essential oil, clove essential oil, wisteria flower essential oil, winter green essential oil, tolu balsam essential oil, patchouli essential oil, rose essential oil, Palmarosa essential oil, cypress essential oil, cypress leaf essential oil, sandalwood essential oil , Pettigrain essential oil, bay essential oil, vetiver essential oil, bergamot essential oil, Peru balsam essential oil, boadrose essential oil, bangjang essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemon grass essential oil, lemon essential oil, Botanical flavorings such as rosemary essential oil and hwajong mint essential oil, and other synthetic fragrances.

Colors and colorants include red cabbage color, red rice color, madder color, anato color, squid ink color, turmeric color, peach color, krill color, persimmon color, caramel, gold, silver, gardenia color, cone color, onion color , Tamarind pigment, spirulina pigment, buckwheat outpost pigment, cherry pigment, seaweed pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple sweet potato pigment, purple yam pigment, rack pigment, rutin, and the like.

Examples of sweeteners include sugar, persimmon tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, and monelin.

Examples of the nutrient fortifying agent include calcined calcium in shells, cyanocobalamin, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron, and the like.

Other hormones, metal ion sequestering agents, pH adjusters, chelating agents, preservatives and flavoring agents, refreshing agents, stabilizers, emulsifiers, animal and vegetable proteins and their decomposition products, animal and vegetable polysaccharides and their decomposition products, animal and vegetable glycoproteins, and Its decomposition products, blood flow accelerators, anti-inflammatory and anti-allergic agents, cell activators, keratin solubilizers, wound treatment agents, foam increase agents, thickeners, oral solutions, deodorants and deodorants, bitter flavors, seasonings, enzymes, and the like are mentioned.

The formulation of the anti-pollution agent of the present invention is arbitrary, in the form of ampoules, capsules, powders, granules, pills, tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, and sheets. It can be used in combination with quasi-drugs such as mousse, cosmetics for skin and hair and bath formulations, food and drink, and pharmaceuticals.

Specific cosmetics and quasi-drugs include, for example, pharmaceutical preparations for internal and external use, lotions, emulsions, creams, ointments, basic cosmetics such as lotions, oils, and packs, facial cleansing and skin cleansing, shampoo, conditioner, hair treatment, Hair cosmetics such as hair cream, pomade, hair spray, hairdressing agent, perm, hair tonic, hair dye, hair growth and wool, foundation, powder, lipstick, cheek touch, eye shadow, eye liner, mascara, eyebrow pencil, Make-up cosmetics such as eyelashes, cosmetics for finishing manicures, perfumes, bath solvents, toothpaste, oral fresheners, mouthwashes, liquid odor and deodorants, hygiene products, sanitary noodles, and wet tissues.

Food and beverages include, for example, soft drinks, carbonated drinks, nutrient drinks, fruit drinks, beverages such as heritage drinks, frozen confections such as ice cream, ice sherbet, and shaved ice, soba, udon noodles, vermicelli, dumpling skin, saomai skin, Chinese noodles, Instant noodles and other noodles, candy, candy, gum, chocolate, sweets, snacks, biscuits, jelly, jam, cream, baked confectionery, bread and other confectionery, crab, salmon, clams, tuna, sardines, shrimp, bonito, Marine products such as mackerel, whale, oyster, saury, squid, saekkomak, scallops, abalone, sea urchin, ikura, and Obunjagi, processed seafood and livestock products such as fish cake, ham, and sausage, dairy products such as milk powder, processed milk, fermented milk, salad Oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, oils and fats processed foods such as dressings, seasonings such as sauce, tare sauce, curry, stew, oyakodon, porridge, vegetable porridge, jukadon, gatsudon, tendon, Eel rice bowl, hiyashi rice, oden, mapo tofu, beef rice bowl, meat sauce, egg soup, omurice, gyoza, sao mai, retort pouch foods such as hamburgers, meatballs, various types of health and nutrition supplements, health functional foods, tablets , Capsules, drinks, troches, and the like.

The anti-pollution agent of the present invention and the external composition for skin containing the anti-pollution agent are suitably applied to humans, but can also be applied to animals other than humans as long as the respective effects can be expected.

The aspect of the external skin composition of the present invention is not particularly limited, but for example, a face wash, a cleaning agent, a lotion (for example, a whitening lotion), a cream (for example, a vanishing cream, a cold cream), an emulsion, a gel, Essence, pack (e.g., jelly-like peel-off type, paste-like wipe type, powdery wash-out type), face mask, cleansing, foundation, lipstick, lip cream, lip gloss, lip liner, cheek touch, makeup foundation, shaving lotion , Sunscreen, after sun lotion, deodorant lotion, body lotion (including hand care lotion and foot care lotion), and body oil. In particular, from the viewpoint of anti-pollution, an external composition for skin used as a daytime leave-on type is preferable, and a lotion, cream, emulsion, gel, essence, foundation, makeup foundation, sunscreen, body lotion, body Oil, etc. are mentioned.

Hereinafter, the present invention will be described in more detail based on examples. In addition, the present invention is not particularly limited to Examples.

Example

(Example 1) Preparation of a plant extract containing polyamine from soybean embryo and/or a plant extract containing polyamine as an active ingredient

Diluted internal standard solution (1,7-diaminoheptane, internal standard amount = 10 μmol) and 5 L of 1N hydrochloric acid solution were added to 1 kg of soybean embryo (mammal), and left at room temperature for 1 hour. Thereafter, 80 g of polycral VT (manufactured by ISP), which is a polyphenol adsorbent, was added, and soybean embryos were sufficiently crushed with a homogenizer, and then allowed to stand at room temperature for 1 hour, followed by extraction under acidic conditions. The lysate was centrifuged at 2°C for 30 minutes at 22,000×g to collect a liquid fraction, neutralized with 30% sodium hydroxide solution, and the main solution was used as a plant extract (soybean germ extract). The plant extract (soybean germ extract) was desalted by an electrodialysis device (acylizer, manufactured by Astom), and concentrated by freeze drying. This powder was dissolved in a 30% butylene glycol solution so as to be 5.0%, and used for various types of evaluation as soybean extraction.

(Example 2) Evaluation of expression inhibitory action on target genes

The soybean extract was used as a test sample, and the expression inhibitory effect on the target gene was evaluated by the following test method. The target gene in this example means an IL1α gene, an IL8 gene, and a CYP1 gene.

Human normal neonatal epidermal keratinocytes (NHEK) were seeded into collagen-coated T75 flasks with Collagen Coating Solution (manufactured by Toyobo Corporation), and normal human epidermal keratinized cell growth medium (HuMedia-KG2) was added. It was cultured in °C and 10% CO ₂ . The medium was appropriately exchanged, and culture was continued for 8 days until 80% or more of total growth was achieved. After removing the medium and rinsing with PBS, trypsin treatment was performed to recover the cells. Cells were seeded so as to be 1×10 ⁴ cells/well in a 96-well plate, and cultured for 1 day at 37°C and 10% CO ₂ .

The medium was removed, and the test sample was replaced with a medium containing a predetermined concentration, and incubated at 37°C and 10% CO ₂ for 5 hours. In addition to the test sample, dexamethasone known as an anti-inflammatory agent was dissolved in DMSO and used as a positive control (final concentration 1 μM). PM2.5 extract (200 mg/ml) was added to a final concentration of 0.2 mg/ml, and incubated for an additional 2 hours. PM2.5 extract was prepared by adding 1 mL of DMSO to 200 mg of urban air dust (NIES CRM No. 28), stirring and centrifuging, and collecting the supernatant. After incubation, the cells were recovered, and cDNA was prepared from the cells using SuperPrep (registered trademark) Cell Lysis & RT Kit for qPCR (manufactured by Toyobo Co., Ltd.).

Based on the obtained cDNA, an mRNA gene was amplified by qRT-PCR using THUNDERBIRD SYBR (registered trademark) qPCR Mix (manufactured by Toyobo Corporation). The reaction solution composition was in accordance with the attached document. However, the amount of reaction liquid was 20 μl/well, and the amount of cDNA was 3 μl. The reaction cycle conditions were 95°C, 1 minute→(95°C, 15 seconds→60°C, 45 seconds)×40→95°C, 15 seconds→60°C, 1 minute→95°C, and 15 seconds. As a primer, an oligonucleotide containing a nucleotide sequence represented by SEQ ID NOs: 1 to 8 was used. The 7500 Fast Real-Time PCR System (Applied Biosystems) was used for gene amplification. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as an internal standard to normalize the expression level of the target gene. A relative value was calculated by dividing the normalized expression level of each target gene by the expression level of the confirmed gene (non-treatment control) in the same manner as above except that PM2.5 was not added.

The obtained results are shown in FIGS. 1 to 3. As can be seen from FIGS. 1 to 3, in the negative control in which the soybean extract was not added in NHEK, the expression levels of the IL1α gene, IL8 gene, and CYP1 gene were increased compared to the untreated control group by particulate air pollutants. Compared with the above, it was confirmed that the expression levels of the IL1α gene, IL8 gene, and CYP1 gene were decreased by adding the plant-derived polyamine-containing extract. From these results, it was found that the plant-derived polyamine-containing extract of the present invention inhibited the increase in expression of the IL1α gene, IL8 gene, and CYP1 gene caused by PM2.5 in epidermal cells, thereby suppressing the inflammatory response.

(Example 3) Preparation of an external composition for skin

Below, a prescription example as the external preparation for skin in this invention is shown.

(1) lotion

According to the following composition, a lotion was prepared by a conventional method.

ㆍPurified water… 89.80g

ㆍGlycerin… 3.00g

ㆍPhenoxyethanol… 0.20g

ㆍButylene glycol… 5.00g

ㆍPentylene glycol… 1.00g

ㆍSoybean extract… 1.00g

(2) gel

According to the following composition, a gel was prepared by a conventional method.

ㆍPurified water… 88.50g

ㆍCarbomer… 0.30g

ㆍXanthan gum… 0.10g

ㆍArginine… 0.40g

ㆍGlycerin… 5.00g

ㆍPhenoxyethanol… 0.20g

ㆍButylene glycol… 5.00g

ㆍSoybean extract… 0.50g

(3) cream

According to the following composition, a cream was prepared by a conventional method.

ㆍPurified water… 58.50g

ㆍButylene glycol… 10.00g

ㆍGlycerin… 5.00g

ㆍPhenoxyethanol… 0.20g

ㆍEthylhexyl glycerin… 0.20g

ㆍSqualane… 10.00g

ㆍOlive U... 10.00g

ㆍBehenyl alcohol… 2.50g

ㆍPolyglyceryl pentastearate-10… 1.90g

ㆍStearoyllactic acid Na… 0.60g

ㆍCetyl palmitate… 1.00g

ㆍSoybean extract… 0.10g

All of these agents are expected to have an effect of suppressing the expression of the IL1α gene, IL8 gene, and CYP1 gene, which are enhanced by PM2.5 in epidermal cells, and are effective as anti-pollution agents. The anti-pollution agent is particularly effective as a component of an external preparation for skin.

SEQUENCE LISTING <110> TOYOBO CO., LTD <120> Antipollution agent and composition of external preparation for skin <130> 18-0021 <160> 8 <170> PatentIn version 3.1 <210> 1 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> forward primer for IL-1 alpha gene in Example 2 <400> 1 ggtcagcacc ttttagcttc ctgagcaa 28 <210> 2 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for IL-1 alpha gene in Example 2 <400> 2 ccgtgaggta ctgatcattg gctcgaa 27 <210> 3 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> forward primer for IL-8 gene in Example 2 <400> 3 agacagcaga gcacacaagc ttctaggac 29 <210> 4 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for IL-8 gene in Example 2 <400> 4 cactccttgg caaaactgca ccttcacac 29 <210> 5 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> forward primer for CYP1 gene in Example 2 <400> 5 tagacactga tctggctgca g 21 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for CYP1 gene in Example 2 <400> 6 gggaaggctc catcagcatc 20 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> forward primer for GAPDH gene as internal standard in Example 2 <400> 7 gcaccgtcaa ggctgagaac 20 <210> 8 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for GAPDH gene as internal standard in Example 2 <400> 8 atggtggtga agacgccagt 20

Claims (6)

An antipollution agent containing a plant-derived polyamine-containing extract as an active ingredient. The method of claim 1, wherein the plant-derived polyamine-containing extract is derived from at least one material selected from the group consisting of soybean seeds, soybean embryos, soybean pears, soybeans, wheat seeds, wheat embryos, wheat pears, wheat malt, soy milk, and soybean oil. Anti-pollution agent, an extract of. The antipollution agent according to claim 1 or 2, wherein the plant-derived polyamine-containing extract is an extract derived from a soybean embryo. The anti-pollution agent according to any one of claims 1 to 3, which contains at least one compound selected from the group consisting of putrescine, spermidine, and spermine as polyamine. The method according to any one of claims 1 to 4, wherein the function of inhibiting the expression of at least one gene selected from the group consisting of interleukin-1α (IL1α), interleukin-8 (IL8), and cytochrome P450 (CYP1) is performed. Anti-pollution agent. A composition for external use for skin containing the anti-pollution agent according to any one of claims 1 to 5.

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