KR20190077831A - The herbicide resistant japonica rice mutant line, Namil(EMS)-gla1 and use thereof - Google Patents

Abstract

The present invention relates to: a Namil mutant progeny system having herbicide resistance, a Namil(EMS)-gla1 rice variety, a method for breeding a rice variety having herbicide tolerance; and a food composition containing the rice variety. The Namil mutant progeny system, Namil(EMS)-gla1, according to the present invention is a rice system that is resistant to herbicides containing glufosinate ammonium, a non-selective herbicide, as an active component. The rice system is effective for reducing efforts for weed control and contributes for reducing an agricultural production cost, thereby expecting to contribute to efficient farm management.

Description

Namil (EMS) -gla1, and its use {The herbicide resistant japonica rice mutant line, Namil (EMS) -gla1 and use thereof}

The present invention relates to Namil mutant herbicide tolerant line Namil (EMS) -gla1 , and uses thereof.

As the demand for food increases with the increase of the world population, increasing the productivity of the crop within the limited land area is one of the important tasks for the stable supply of food. Under these technical backgrounds, the occurrence of weeds in cropland causes crop yield reduction and quality degradation due to water and nutrient competition with crops. The reduction in the yield of crops due to weeds has been reported to be in the range of about 30 to 100%, though there is variation depending on the crops. Therefore, the control of weeds as a technology for increasing the productivity of the crops takes a very large proportion . In particular, the use of herbicides for the control of weeds is essential for the reduction of agricultural production costs due to the labor shortage and aging of the rural population in recent years.

However, recent ongoing use of single herbicides has increased the incidence of herbicide-resistant weeds. There have been reports of herbicide-resistant weeds in more than 235 ferns in 65 countries worldwide and more than 50 fungicides resistant to phenoxy clock herbicides have been reported in paddy fields. In Korea, there have been reported herbicide-resistant weeds in 9 species of perennial weeds and 4 weeds in perennial weeds since the report of waterborne grasses resistant to sulfonylurea herbicides in Seosan reclaimed paddy field in 1998. In 2009, Blood that is resistant to the -COA carboxylase inhibitor and the acetolactate synthase inhibitor has been reported.

Effective management of herbicide-resistant weeds is to avoid alternate use of herbicides by alternating herbicides with different mechanisms of action, and to convert them into resistant crops for each herbicide.

The development of herbicide resistant crops has been developed in various crops such as corn, rapeseed, soybean, cotton, rice, wheat, sugar beet, etc. since 1996 when Monsanto developed glyphosate herbicide resistant soybeans and Roundup Ready. In 1993, imidazoline seed mutation treatment was applied to rice, and imazethapyr, a herbicide for imidazoline resistant rice, was developed and used as a soil treatment agent and an antimicrobial treatment agent before and after sowing on rice.

On the other hand, weed rice is physiologically and morphologically similar to cultivated rice, so it is difficult to control it with conventional selective herbicide, and it is a big problem especially in direct seed culture. Clearfield ^® Rice is a herbicide resistant rice varieties resistant to the imidazoline (IMI) herbicide, a non-selective herbicide. It is resistant to mutagenic mechanisms of Acetohydroxyacid or Acetolactate synthase enzyme (ALS) It is commercialized and cultivated in 15 countries including USA, South America and Asia. However, recently, the emergence of weedy rice showing tolerance to IMI herbicides has been reported mainly in the tropics, and accordingly, the risk of herbicide resistant weed rice caused by the use of Clearfield ^® Rice is highlighted.

Glyphosinate ammonium herbicides are widely used as non-selective herbicides and have excellent control effect on weeds and are known to be very effective especially for herbaceous plants. Because of the rapid deactivation of soils in the soil and the incidence of resistant weeds is significantly lower, the cultivation area of glucosamine ammonium herbicide resistant crops is increasing, and at present, glucosinic ammonium herbicide resistant crops include sugar beets, rapeseeds, tomatoes, potatoes Have been developed.

There have been a number of cases of resistance research on domestic herbicides, but none have reached the practical stage. At present, the rice with PAT gene and MxPPO has progressed to the stage of stability evaluation, and the ISTANBUL RESISTANCE Iksan 483 and Milyang 204, which were produced through hybridization of the GM rice strain developed by transforming the Basta resistance gene and domestic varieties, (Korean Patent No. 10-0734012), but it is difficult to be distributed to actual farm households. Therefore, it is urgent to develop a herbicide tolerant rice varieties which can be used in farmhouses.

SUMMARY OF THE INVENTION The present invention has been made in order to solve the above problems, and the present inventors have made intensive efforts to develop a herbicide, specifically, a glucosinic ammonium herbicide tolerant rice variety. As a result, The present invention has been completed on the basis of this finding, confirming excellent resistance to non-selective herbicides containing Namil (EMS) -gla1 gluconate ammonium as an active ingredient.

It is an object of the present invention to provide a rice varieties of mutant rice varieties having herbicide tolerance.

Another object of the present invention is to provide a method of breeding rice varieties having herbicide resistance, which comprises selecting rice varieties having herbicide tolerance among the seeds of each line established from the south-south mutant line.

It is still another object of the present invention to provide a food composition containing the rice varieties and / or seeds thereof as an active ingredient.

However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention as described above, the present invention provides a "Namil (EMS) -gla1 " rice varieties having a herbicide tolerance of the accession number KACC 98048P.

In one embodiment of the present invention, the herbicide may be a herbicide containing gluconate ammonium as an active ingredient, and may be, for example, Basta.

In another embodiment of the present invention, the rice varieties may have the following characteristics.

Heading: July 29 ± 10 days

Soy (cm): 77 ± 20

Length (cm): 28 ± 10

Sorghum (dog): 7 ± 5

Brown rice gin (g): 25.8 ± 5.0

Brown rice length (mm): 5.72 ± 3.00

Width of brown rice (mm): 2.94 ± 2.00

Brown rice width ratio: 1.95 ± 0.50

In addition, the present invention relates to a method for growing M2 seeds harvested from a plant (M1) grown by germination by immersing Nanyang rice seeds in an ethyl-methane-sulfonate diluted solution, Thereby establishing a fixed mutation system; And selecting the rice varieties having herbicide tolerance among the seeds of the respective established strains. The present invention also provides a method for breeding rice varieties having herbicide resistance.

In one embodiment of the present invention, the herbicide may be a herbicide containing gluconate ammonium as an active ingredient, and may be, for example, Basta.

In addition, the present invention provides a food composition comprising the above-mentioned rice varieties and seeds thereof as an active ingredient.

Damn mutant progeny strains Namil (EMS) according to the invention - gla1 is a rice strains resistant to herbicides containing phosphorus, glufosinate ammonium non-selective herbicides as active ingredients, the rice system to reduce the effort for a herbicidal And contributing to the reduction of agricultural production costs, it is expected to contribute to efficient farm management.

Fig. 1 shows the result of a simplified assay for selection of a mutation line system having a herbicide tolerance of the present invention.
Fig. 2 shows the result of assaying the herbicide (Basta) resistance of the Namil (EMS) -gla1 lineage system of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a "Namil (EMS) -gla1 " rice varieties having a herbicide tolerance to the south- south mutant lineage.

As used herein, the term "herbicide " refers to a chemical agent that inhibits or modulates the growth of a plant, and is classified as a non-selective (detoxic) herbicide and a selective herbicide depending on the application range of the herbicide. Here, "non-selective" means that the plant species in which the herbicide is active is not selective and has activity against all plant species. For purposes of the present invention, the herbicide preferably contains the active ingredient glufosinate ammonium , And more preferably Basta.

The herbicide (glufosinate ammonium) herbicide, which is one of the non-selective herbicides, is excellent in the control effect of weeds, has a remarkably low occurrence frequency of resistant weeds, and is rapidly inactivated in the soil and is not accumulated in the soil, The cultivation of crops is increasing. Therefore, development of the above-mentioned Basta herbicide-tolerant rice varieties is urgent in terms of efficient control of weeds and reduction of production labor. Accordingly, the present invention has a technical feature in that it provides a novel rice variety having resistance to the above-mentioned Basta herbicide.

Rice is a chosen varieties through Basta resistant black experiment from damn rice mutant fixed clade (total of 2888 strains) by treatment of ethyl methane sulfonate (see Examples 1 and 2) in accordance with the present invention, the varieties Oryza sativa Namil (EMS) - gla1 , It was deposited with the National Institute of Agricultural Science and Technology (RDA) of the Rural Development Administration and received grant number KACC 98048P on December 11, 2017.

The rice varieties according to the present invention exhibit very stable Basta herbicide tolerance and have similar crop characteristics to those of the original rice varieties (see Table 1). Therefore, the application of non-selective herbicides will effectively control weed rice and weeds and contribute to the reduction of production labor for cultivation of crops.

In another aspect of the present invention, the present invention relates to a method for producing a seed, which comprises culturing a rice seed obtained from a plant (M1) cultivated by immersing a rice paddy seed in a dilute ethyl methane-sulfonate solution, To a M7 generation to establish a fixed mutation system; And selecting the rice varieties having herbicide tolerance among the seeds of the respective established strains. The present invention also provides a method for breeding rice varieties having herbicide resistance.

In the present invention, the herbicide may be a non-selective herbicide containing gluconate ammonium as an active ingredient, more preferably Basta, but is not limited thereto.

In the present invention, the step of establishing the fixed mutation system and the step of selecting the rice herbicide resistant to the herbicide resistance may be carried out in accordance with changes in the kind of the herbicide, the characteristics of the mutant strain, and the like. In one embodiment, the step of selecting the rice varieties is carried out by applying a herbicide at a specific concentration (ex, 0.5X and 1X) and cultivating the herbicide. The degree of tolerance is visually evaluated at the time when the weakness of the original cultivar, However, the present invention is not limited thereto, and means known in the art may be further included.

Further, as another embodiment of the present invention, the present invention provides a food composition comprising the aforementioned rice varieties and / or seeds thereof as an active ingredient.

The food composition of the present invention includes all forms of rice processed foods, functional foods, nutritional supplements, health foods, and food additives. Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art.

For example, rice processed foods include rice cake (rice cake, rice cake rice cake, rice cake rice cake, noodle, ramen noodle, noodles, raw noodles, traditional rice cake), rice confection (biscuits, biscuits, snacks, (Sweet potato, soju, beer, sake), seasoning (meats, soy sauce, vinegar) and other rice processed products (such as seaweed, sikhye, snack ingredients, Beans, rice bran, processed rice) were added to Namil (EMS) -gla1 It can be produced by processing rice varieties (plants) or seeds thereof. Also, the health food can be prepared by preparing Namil (EMS) -gla1 rice plants or seeds of the present invention in the form of tea, juice and drink and drinking, granulating, encapsulating and pulverizing. As a food additive, Namil (EMS) -gla1 rice plants or seeds of the present invention can be used in the form of powders or concentrates.

The desirable content of the Namil (EMS) -gla1 rice plant or the seed thereof in the food composition of the present invention is not limited thereto, but may preferably be 0.01 to 95% by weight in the finally prepared food.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the following examples.

[ Example ]

Example  1. Selection of rice varieties with herbicide tolerance

1-1. Namil Paddy  Mutation Group of fixed systems  Establish

A mutant line was obtained in which rice seeds of Oryza sativa L. sp . Japonica were treated with Ethyl-methane-sulfonate diluted in phosphate buffer solution as a mutagen. 1 seeds were harvested from M1 plants (M2 seed), and from then on, up to M7 generation, a system was established by advancing the generation based on the system breeding method. One plant with high fidelity per plant was randomly selected for each generation and the succeeding plant was developed by one - water one - column method. A total of 2,888 lines were identified in the M7 plant genera, judging that individuals within each lineage exhibited a homogeneous phenotype and were genetically fixed.

1-2. Selection of Rice Varieties Resistant to Herbicide Resistance

In order to secure lines expressing resistance to the non-selective herbicide Glufosinate ammonium, experiments were carried out on a group of mutant strains of Nam-il rice (all 2,888 strains) of Example 1-1 at all times.

First, in this embodiment, 1X, which is a dose included in the recommended dose of VASTA, and 0.5X, which is reduced to 1/2, are used together. It is estimated that the herbicide sales company can not sufficiently apply the herbicide spraying amount (spraying 100 liters of diluent to 300 pyeong, that is, treating 10 m ² with 1 liter of drug diluent). , The concentration was diluted to 1/2, and the amount of the medicament to be treated was adjusted to be the same. That is, a 0.15% dilution of a commercially available drug VASTA (effective dose: 18%) was used based on the 1X herbicide drug, but the dose was doubled. 0.5X herbicide medicines were diluted with water to 1/1 ratio, and rice varieties resistant to Basta were selected.

Example  2. Herbicide resistance Namil (EMS) - gla1  Selection of rice varieties and investigation of crop characteristics

In order to evaluate the above-mentioned Vasta-resistant strains, the best-prepared seedlings were sown in a seedling box of 0.16 m ² area, seeded for 2 to 3 weeks, sprayed with 0.5X and 1X agent, , Sambrook et al.), The degree of tolerance to Basta was assessed by the questionnaire. In addition, these herbicide - tolerant mutants were evaluated for their herbicide tolerance and their major crop characteristics such as heading, soybean, and shoots were reported in the Jeonju test package of the National Institute of Food and Nutrition.

As a result, as shown in FIG. 1 and FIG. 2, the rice varieties selected in Example 1 at all times showed relatively good tolerance to the non-selective herbicide Busta. Namil (EMS) M2-180-1-1 -1-1-1 '(86152 (R), Namil (EMS) - gla1 ) showed very stable buster herbicide tolerance.

As shown in Table 1, the Namil (EMS) -gla1 rice varieties according to the present invention were found to be resistant to Basta herbicide resistance and to be resistant to crops such as soybean, And it was confirmed that the characteristics were similar to those of Namil rice.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.

Rural Development Administration National Institute of Agricultural Science KACC98048P 20171211

Claims (7)

"Namil (EMS) - gla1 " rice varieties (KACC 98048P) which are resistant to herbicides containing glufosinate ammonium as an active ingredient.
The rice variety according to claim 1, wherein the herbicide is Basta.
The rice varieties according to claim 1, wherein the rice varieties have the following characteristics.
Heading: July 29 ± 10 days
Soy (cm): 77 ± 20
Length (cm): 28 ± 10
Sorghum (dog): 7 ± 5
Brown rice gin (g): 25.8 ± 5.0
Brown rice length (mm): 5.72 ± 3.00
Width of brown rice (mm): 2.94 ± 2.00
Brown rice width ratio: 1.95 ± 0.50
The M2 seeds harvested from the plant (M1) grown by germination were immersed in an ethyl-methane-sulfonate dilution solution of the rice seeds of Namil, and the seeds were advanced to the M7 generation according to the systematic breeding method to obtain a fixed mutant strain ; And
Selecting a rice varieties resistant to herbicides among the seeds of each of the established strains; and cultivating the herbicide tolerant rice varieties.
5. The method according to claim 4, wherein the herbicide is a herbicide containing ammonium gluconate as an active ingredient.
5. The method according to claim 4, wherein the herbicide is Basta.
A food composition comprising the rice varieties of any one of claims 1 to 3 or a seed thereof as an active ingredient.

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