KR20190068361A - Composition comprising Plant Extract Complex for Anti-bacteria, Anti-inflammation, Anti-oxidation, Inhibiting Sebum Secretion and Improving Dermatitis - Google Patents

Abstract

The present invention relates to a composition capable of being antibacterial, anti-inflammatory and antioxidant, suppressing sebum secretion, and alleviating dermatitis by including a plant extract mixture and, more specifically, to a composition capable of being antibacterial, anti-inflammatory and antioxidant, suppressing sebum secretion, and alleviating dermatitis by including a mixture of five plant extracts which are Prunella vulgaris, Magnolia liliflora, Glycine soya, Glycyrrhiza glabra, and Centella asiatica as active ingredients. According to the present invention, the composition including the extract mixture as an active ingredient has effects of: inhibiting the growth of microorganisms related to skin problems, preventing the oxidation of skin cells caused by active oxygen, alleviating skin inflammation, and controlling sebocyte division and sebum secretion by having excellent antibacterial, antioxidant, anti-inflammatory, and sebum secretion suppressing effects; and alleviating dermatitis by reducing the oil content of sebum, the value of sebum secretion, and the number of closed comedos and open comedos on atopic or acne-prone skin. Moreover, the composition can be used safely even for long-term use since the composition has little toxicity and side effects, and particularly, can be safely applied to cosmetic materials and a pharmaceutical composition.

Description

[0001] The present invention relates to a composition for inhibiting antimicrobial, anti-inflammation, antioxidant, sebum secretion and dermatitis, which comprises a plant extract mixture,

The present invention relates to anti-bacterial, anti-inflammatory, antioxidant, sebum secretion inhibitory and dermatitis improving composition comprising a plant extract mixture, more specifically, Lamiaceae (Prunella vulgaris), jamokryeon (Magnolia liliflora), dolkong (Glycine soya), licorice ( Glycyrrhiza glabra , Centella asiatica , and the like. The present invention also relates to a composition for improving antimicrobial, anti-inflammatory, antioxidative, sebum-inhibiting and dermatological properties.

The skin of the human body is the largest organ among the organs constituting the body, and it is usually divided into the epidermal layer, the dermal layer and the subcutaneous fat layer. The skin is always exposed directly to the external environment, so it can receive various stimuli according to the environmental change, and due to the stress or defense mechanism, various skin diseases may be manifested. The skin is the outermost organ of the human body and functions as a first bio-protective membrane. It prevents moisture loss in the human body, simultaneously blocks the penetration of various harmful substances and contaminants that can be introduced from the outside, Protects the human body from heat by reducing the heat loss through shrinkage in cold environments, and releasing sweat in hot environments to dissipate body heat.

Such skin may be caused by biological factors such as severe fine dust or various pollutants or changes in the environment, such as changes in temperature, changes in the external environmental factors, physical factors such as abrasions or strong sun ultraviolet rays, insect bites such as mosquitoes, bees, mites, , Burning, inflammation, edema, and the like due to various factors such as abnormalities of the endocrine system, skin irritation, dryness, pruritus (irritation), irritation, burning, Branch skin diseases can be expressed.

Among many of these skin diseases, chronic dyspepsia (atopic dermatitis) and acne dermatitis (acne dermatitis) are the most common diseases that have not yet been identified.

First, atopic dermatitis is a typical contact, allergic dermatitis, itchy, dry and sensitive to the skin, giving many people suffering. Although it occurs mainly in children less than 5 years old, it is not limited to children but it can occur in all ages. When atopic dermatitis occurs, pruritus (itching) frequently causes scratching and scarring. Tea infections can occur. Symptoms that appear are red rashes, dirt, bleeding, and exaggeration. It usually occurs in areas where the skin touches, ie, inside the elbow, inside the elbow, behind the knee, in the groin, underarm and neck. And may appear at any particular site, including.

The incidence of atopic dermatitis is increasing as the day progresses. Major factors are environmental pollution caused by industrialization, and the increase of Allergen (a representative example of fine dust), which is the cause of allergy in the air, Changes in the eating habits such as house dust mites, pollen, fungus, animal hair, cockroaches, etc. caused by the housing environment and meat and high fat, high calorie food intake, family history as a contact antigen, And biorhythm abnormality due to lack of sleep due to lack of sleep. Thus, atopic dermatitis is not caused by any specific cause, but is caused by various and complex causes.

The pathogenesis of atopic dermatitis has not yet been elucidated, but it is linked to innate genetic factors as well as immunological and nonimmunological mechanisms. It is known that exogenous dermatitis, which accounts for the majority of atopic dermatitis, is caused by an immune mechanism related to immunoglobulin E (IgE), which does not appear as an immediate immune response to certain allergens but by a progressive immune response by T- have. More specifically, allergen is injected through the horny layer of the skin and then reaches the epidermal cells and is degraded by macrophages, a type of leukocyte. At this time, when the amount of allergen introduced is large, macrophages can not be processed, and allergens remain. As a result, the eosinophils are transferred to the eosinophil and the eosinophils, which are widely distributed in the blood, are concentrated into the place where the allergen has penetrated, Inflammation is caused by overexposure.

Atopic dermatitis starts from an infantile eczema termed as a fever. In the acute phase, the skin becomes itchy, reddened, swollen or blistered due to pruritus. In the chronic phase, the skin may become hardened or thickened, It turns black. The bigger problem is that these symptoms do not end in a one-shot, but repeatedly.

Treatment of atopic dermatitis basically alleviates skin dryness and pruritus. It mainly uses antihistamine, local or systemic steroids, and additionally uses skin infection control agent, ceramide, far infrared ray or light wave treatment of specific wavelength It is also said. Steroids are excellent for relieving inflammation and immunosuppression, and antihistamines can help relieve pruritus by preventing the release of histamine from mast cells. However, long-term use of steroids and antihistamines may cause side effects such as hair growth at the site of application, skin atrophy, skin pigment reduction, acne, metabolic disorder, growth disorder, gastric mucosal dilation, gastric ulcer, osteoporosis, have.

On the other hand, acne is an inflammatory disease that occurs in the sebaceous glands of the skin in the skin. It occurs mainly in adolescents with severe hormonal changes, but it also occurs in adults between the ages of 20 and 40 because of adult acne. As with atopy, its cause has not yet been clearly identified, but it is also known to be caused by changes in hormone secretion in the body, in addition to genetic and environmental factors.

Especially, testosterone, which is one of the male hormones (Androgen), causes excessive secretion of sebum and clogs the pores due to overgrowth of the sebaceous glands. When the pores are closed, ( Propionibacterium acnes ), which is a typical acne bacterium, is proliferated. The sebum accumulated in the closed pores is decomposed into free fatty acids by the lipase Lipase, the lipase secreted by the acne bacterium. In addition to the free fatty acids and the other low molecular substances secreted by the acne bacteria, white blood cells surround the hair follicles As they are gathered, the stimulation and destruction of the hair follicle wall causes the contents of the hair follicles to flow into the dermis and cause an inflammatory reaction. In addition to male hormones, histamine (Histamine), insulin-like growth factor I (IGF-I), and peroxisome proliferator- activated receptors. Indirect factors that cause acne include pore clogging due to environmental pollution, increased steroid hormone and androgen secretion due to stress, deterioration of inflammation caused by lack of sleep due to lack of sleep or drinking, and ingestion of high fat foods or oil Use of cosmetics containing a lot of ingredients, and lifestyle habits that are not clean.

To treat these acne, there are four main ways to treat acne: how to inhibit sebaceous gland secretion, how to normalize follicular keratinization, how to inhibit the growth of acne bacterium by antimicrobial action, and how to relieve inflammation have. First, antiandrogens (spironolactone, flutamide, ketoconazole, finasteride, etc.) may be used to inhibit sebaceous hyper-secretion. Second, salicylic acid, glycolic acid, and retinoic acid are used to normalize follicular keratin. Third, antimicrobial agents such as tetracycline, minocycline, doxycycline, erythromycin, clindamycin, azelaic acid, triclosan, resorcinol, sulfur and the like are used. Finally, anti-inflammatory agents such as licorice, resveratrol, rosemary, salicin and other antioxidants are used. However, anti-androgens, antibiotics, and antimicrobial agents are believed to cause various side effects such as epidermal growth inhibition, birth defects, skin irritation, contact dermatitis, allergic reactions, photosensitivity reactions, skin redness, edema, .

Recently, interest in research on natural products including plants has been increased to reduce side effects in acne treatment. Natural products known to have antibacterial and antiinflammatory actions include bee venom, green tea, tea trioyl, aloe, chitosan, , Willow, plum, golden, licorice, saury, mushroom, mackerel, mackerel, papaya, roe, crowd, bamboo salt.

In this regard, Korean Patent No. 10-1648187 (registered on Aug. 20, 2016) discloses that a mixture composed of Korean herb, oriental herb, oriental herb, oriental herb, Korean Patent Laid-Open No. 10-2016-001267 discloses a cosmetic composition for improving atopic dermatitis, which comprises an extract of Ganoderma lucidum extract as an active ingredient, and a cosmetic composition for improving atopic dermatitis And a manufacturing method thereof. Korean Patent Laid-Open No. 10-2012-0138066 (published on Dec. 24, 2012) discloses a herbal composition for improving acne and acupuncture in which a mixture of Sukjunghwang, Gosam, Garlic, Gugija, Black Soybean Fermented Extract and Aloe Extract Korean Patent Laid-Open No. 10-2007-0105146 (published on Oct. 30, 2007) discloses a cosmetic for preventing and treating acne which suppresses the growth of Propionebacterium acnes by using a dryopteris crassirhizoma extract. The composition is described. However, the natural extracts of these patent documents have a disadvantage in that they are less effective than the existing atopic dermatitis treating substances or acne treating substances in terms of effectiveness.

Accordingly, the present inventors have continued research on natural products that can treat, ameliorate, and prevent various dermatitis such as atopic or acne dermatitis which are harmless to human body, and have antimicrobial, antioxidant, anti-inflammatory and anti- This medicine improves skin dryness, fever, itching and exaggeration. It suppresses the proliferation of Propionibacterium acnes , which is the main cause of worsening acne symptoms, and reduces sebaceous hyperplasia caused by excessive secretion of male hormone. The present invention has been completed by developing a composition capable of improving skin diseases such as atopic dermatitis and atopic dermatitis.

Therefore, one technical problem to be solved by the present invention is to provide an extractive mixture using Prunella vulgaris , Magnolia liliflora , Glycine soya , Glycyrrhiza glabra and Centella asiatica as an active ingredient And to provide a composition for improving various dermatitis including atopic or acne dermatitis which has antibacterial, antioxidant, anti-inflammatory and anti-sebum secretion-inhibiting effects.

In one embodiment, the present invention relates to a method for producing an extract comprising an extract mixture obtained by extracting from Prunella vulgaris , Magnolia liliflora , Glycine soya , Glycyrrhiza glabra and Centella asiatica , Antimicrobial, anti-inflammatory, antioxidant, anti-sebum secretion and dermatitis improvement.

Prunella vulgaris is a perennial herbaceous plant of the dicotyledonous plant that grows in mountains and fields in each region of Korea and is also called 'eggplant herb' and its generic name is' to be. The leaf is about 2 ~ 5cm long and has long egg-shaped or long elliptical shape. The stem is square and the whole has short hairs. The flower is reddish violet, 3 ~ 8cm long, and flowers are piled up on the stem, and the petals coming forward are like lips. The fruit runs in tan from July to August, and the pod remains dry and remains in the fall. In one room, Lamiaceae are used as gonorrhea, tuberculosis, inflammation, boils and diuretic. In the present invention, it is preferable to use at least one of flowers, leaves and stem of Lamiaceae.

Magnolia liliflora is a naturalized plant that came from China with magnolia about 100 years ago. It is a deciduous tree with dicotyledonous plants and buttercups. It is mostly planted for ornamental purposes, but its height is 15m and its branches are divided much, and the petiole length is 7 ~ 15mm. Leaves are opposite to each other and look like inverted eggs. The edges are flat, with hairs on both sides, but gradually disappear as they grow. Flowers bloom earlier than leaves in April, with black purple, fruit oval, brown in October, and a triangular red seed appear to hang on a white thread. In one room, the pre-flowering buds of the lumberjack are called ginseng (辛 荑) or ginseng (辛 荑 花). They are mainly used for treating headache, toothache and rhinitis. In the present invention, it is preferable to use buds of buds (buds).

Glycine soya is a perennial herbaceous perennial plant that grows in the mountains and branches of each region of Korea. The growing environment grows in semi-shade or sunny soil regardless of soil fertility. The key is about 2m in length, the leaves are alternate phyllotaxis, the petiole length is about 7 ~ 15cm and the short hairs are. The flower is light purple, 2 ~ 5cm long, bloomed and about 6mm long. The fruit is mature in September and the seed is kidney-shaped, dark brown and similar to small bean. In the present invention, it is preferable to use seeds (seeds) of dolomite.

The licorice ( Glycyrrhiza glabra ) is a perennial herbaceous plant with soybean and is native to southern Europe and India. It grows up to about 1 meter in height, its leaves are pinnate, and its length is 7 ~ 15cm with 9 ~ 17 leaves. The flowers are about 0.8 ~ 1.2cm in size and have a light blue color which is purple or white. Fruits are 7 ~ 8 seeds in 2 ~ 3cm oval pods. Glycyrrhiza is effective in cleansing the body by discharging heavy metals and toxic substances accumulated in the body. It helps to prevent food poisoning and drug poisoning. In particular, Glycyrrhizic acid is useful as a diphtheria toxin, a tetanus toxin, And is known to help break down and detoxify nicotine and alcohol. In the present invention, it is preferable to use licorice roots.

Centella asiatica is a perennial herbaceous plant of the dicotyledonous plant. It is better known as 'tiger grass' because it has rolled over when the tiger was injured. It is mainly distributed in Asia, and it grows in somewhat humid places. There are two degenerated scaly leaves near the node, extending from side to side and lowering from the node. Normal leaf is dense in the nodule, petiole is long, oval, diameter is 2 ~ 5cm, and the edge is serrated. Flowers bloom in July to August in reddish violet, 2 ~ 5 flowers hang on the end of long peduncle, 5 petals are broad circle, and 5 stamens. The fruit is flat, round and has a net that protrudes from the surface. It has hairs at first but disappears later. In Korea, it is common in mountainous areas in southern islands such as Jeju Island. In the present invention, it is preferable to use leaves, stems, or both leaves and stems of the centipedes.

The term "plant extract" in the present invention refers to any extract, fraction, purified product, diluted solution, concentrate, or dried product obtained from each step of extraction, fractionation or purification (separation, fractionation) of Lamiaceae, Or any form that is formulated using the same.

The plant extract can be obtained using an extraction method and an extraction solvent known in the art. The extraction solvent may be selected from the group consisting of water, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide, dimethylsulfoxide (DMSO) , 1,3-butylene glycol, propylene glycol or a mixed solvent thereof. The degree of extraction and loss of the active ingredient of the plant extract may vary depending on the extraction solvent to be extracted. Preferably, alcohol such as methanol, ethanol, isopropanol, and butanol is used, more preferably 70 to 100% Use ethanol as a solvent.

The extraction method is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction and the like can be used. Extraction can be carried out by room temperature or warming under the condition that the active ingredient of the extraction mixture is not destroyed or minimized.

In addition, the plant extract may be obtained by a conventional purification process in addition to the extraction method using the above-mentioned extraction solvent. For example, various purification processes have been carried out, including ultrafiltration with a constant molecular weight cut-off value, freezing filtration, centrifugation, various chromatography (size, charge, hydrophobicity or affinity chromatographic separation) But the present invention is not limited thereto.

In the present invention, the term "extraction mixture" means any extract, fraction, purified product, diluted solution, concentrated solution obtained by extracting, fractionating, or purifying (separating or fractionating) a mixture of Lamiaceae, , Dried form or all forms formulated with it.

The above-mentioned "extraction mixture" may be any extract, fraction, purified product, diluted solution, concentrate, dried product or the like obtained in each step of extraction, fractionation or purification (separation, fractionation) of Lamiaceae, Which is a mixture of all formulated forms.

In one specific embodiment, each of the plant extract powder is prepared by dissolving each plant extract powder in a hydrophilic solvent in the case of preparing an extract mixture by extracting and laminating each of Lamiaceae, Lycoris, Lycoris, Licorice and Cinnabar. This is because when the plant extract is pulverized, it is poorly water-soluble. When the extract mixture is prepared in the form of a liquid preparation using a hydrophilic solvent, it can be applied to cosmetics or medicines of water-soluble formulations.

The hydrophilic solvent includes, but is not limited to, aliphatic or aromatic alcohols; Aliphatic or aromatic alkoxylated alcohols; Glycol or alkoxylated glycols; And mixtures thereof, preferably propanediol, butylene glycol, and glycerin. More preferably, propanediol is used. By using the hydrophilic solvent as described above, the penetration ability of the extracted mixture can be increased, so that the active ingredient can easily penetrate into the skin, and the skin moisturizing power can be increased.

The extracted mixture is prepared by mixing 1 to 20 parts by weight of a crude extract, 10 to 70 parts by weight of a spinach extract, 60 to 140 parts by weight of a licorice extract, and 160 to 240 parts by weight of a centurion extract, based on 100 parts by weight of an extract of Lamiaceae. Preferably, 5 to 15 parts by weight of a crude extract, 20 to 60 parts by weight of a spinach extract, 70 to 130 parts by weight of a licorice extract, and 180 to 220 parts by weight of a centipede extract are mixed, based on 100 parts by weight of an extract of Lamiaceae. More preferably, 7 to 12 parts by weight of the crude extract, 30 to 50 parts by weight of the spinach extract, 90 to 110 parts by weight of the licorice extract, and 190 to 210 parts by weight of the centipede extract are mixed based on 100 parts by weight of the extract of Lamiaceae. When the content of each extract is out of the above range, the improvement effect on atopic and acne dermatitis is small or not, and it is not completely dissolved in the mixed solvent, and the precipitate appears when the product is applied.

In the present invention, "antibacterial" means an action of inhibiting or controlling the growth and proliferation of microorganisms such as bacteria or fungi, and preferably means an action of inhibiting or controlling the growth and proliferation of the skin bacteria.

In the present invention, "antioxidant" means an inhibition, improvement, or control of destruction or damage of skin cells, biologic lipids, phospholipids, proteins, DNA and the like caused by active oxygen.

In the present invention, the term "anti-inflammatory" is intended to mean an inhibition of inflammation formed by the infestation of external infectious agents (bacteria, fungi, viruses, various kinds of allergenic substances), delay of onset of inflammation, It means all actions that change.

In the present invention, "inhibition of sebum secretion" means inhibiting or modifying sebocyte differentiation to inhibit, improve or treat oily skin.

In the present invention, "dermatitis" refers to symptoms such as inflammation, redness, swelling, fever, and pain caused on the skin, and includes atopic dermatitis, acne dermatitis, primary irritant dermatitis, contact dermatitis, seborrheic dermatitis, , And linear dermatitis, and preferably refers to atopic dermatitis or acne dermatitis.

In the present invention, "improvement" means (a) inhibiting (b) abatement and (c) elimination of development of dermatitis diseases in an individual, and includes the meaning of "prevention ".

The term "prevention" as used herein refers to inhibiting the occurrence of a disease or a disease in an individual who has never been diagnosed as having a disease or disease, but who tends to be susceptible to such disease or disease.

In the present invention, the term "individual" means any animal, including a human having a disease whose symptoms may be improved by administering the anti-inflammatory composition of the present invention, such as a monkey, a cow, a horse, a pig, a sheep, a dog, a cat, Means a mammal such as a chimpanzee.

In one specific embodiment, the composition for improving antibacterial, antioxidant, anti-inflammatory, sebum secretion and dermatitis of the present invention can be used as a cosmetic composition.

The cosmetic composition of the present invention contains the extract mixture in an amount of 0.5 to 20% by weight, preferably 1 to 10% by weight, more preferably 2 to 6% by weight, based on the total weight of the cosmetic composition. If the amount is less than 0.5% by weight, the skin-improving effect of acne is insufficient. If the amount is more than 20% by weight, the increase in the effect of the composition is insignificant.

The cosmetic composition may further include components commonly used in cosmetic compositions in addition to the above-mentioned extraction mixture. For example, conventional auxiliaries and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and antioxidants.

The cosmetic composition may be prepared in any form conventionally produced in the art. For example, it can be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray. More specifically, it can be prepared as a nutritional cream, a convergent lotion, a soft lotion, a lotion, an essence, a nutritional gel or a massage cream. However, the present invention is not limited thereto.

When the formulation of the cosmetic composition is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component. For example, fatty acid esters of water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used have.

When the formulation of the cosmetic composition is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention exhibits excellent antibacterial, antioxidant, anti-inflammatory, anti-sebum secretion and dermatitis-improving effects without cytotoxicity.

In another specific embodiment, the composition of the present invention can be used as a pharmaceutical composition.

The pharmaceutical composition of the present invention contains the extract mixture in an amount of 0.0001 to 50% by weight, preferably 0.001 to 30% by weight, more preferably 0.01 to 20% by weight, based on the total weight of the pharmaceutical composition . If the amount is less than 0.0001% by weight, the effect of improving acne skin is insufficient. If the amount is more than 50% by weight, the increase in the effect of the composition is insignificant.

The pharmaceutical composition of the present invention may be prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art, Into the container. The formulations may be in the form of solutions, suspensions, syrups or emulsions in an oil or aqueous medium, or in the form of excipients, powders, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

The pharmaceutically acceptable carrier to be included in the pharmaceutical composition of the present invention is a compound usually used for the presentation of a pharmaceutical composition and includes a carbohydrate-based compound (for example, lactose, amylose, dextrose, sucrose, sorbitol, mannitol, starch , Cellulose, etc.), acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, salt solution, alcohol, gum arabic, vegetable oil But are not limited to, polyvinylpyrrolidone, polyethylene glycol, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like.

The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be formulated in the form of injection or external preparation according to a conventional method.

The pharmaceutical composition of the present invention has sebum reduction and skin soothing effect, and the administration method comprises administering the pharmaceutical composition in a pharmaceutically effective amount to mammals such as rats, mice, livestock, and humans in various routes.

The pharmaceutical composition may be administered orally or parenterally, preferably by parenteral administration, more preferably topical application by application.

A suitable dosage of the pharmaceutical composition of the present invention may vary depending on such factors as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, . The dosage of the pharmaceutical composition of the present invention is in the range of 0.001-100 mg / kg on an adult basis. When the composition is an external preparation, it is preferably applied in an amount of 1.0 to 3.0 ml on an adult basis once to five times a day, and continued for 1 month or more. However, the dose is not intended to limit the scope of the present invention.

As described above, the composition comprising the extract mixture of Lamiaceae, Lycorrhoids, Dolomands, Licorice and Cinnabar preparations as an active ingredient according to the present invention is excellent in antibacterial, antioxidant, anti-inflammatory and anti-sebum secretion suppressing effects, (Sebaceous glands), sebaceous glands, sebaceous glands, sebaceous glands, sebaceous glands, sebaceous glands, sebaceous glands, sebaceous glands, sebaceous glands and sebaceous glands. It shows the effect of improving. In addition, since the composition according to the present invention has little toxicity and side effects, it can be safely used even in long-term use, and can be safely applied to cosmetic compositions and pharmaceutical compositions.

FIG. 1 is a graph showing the results of measuring antioxidative effects.
FIG. 2 is a graph showing the results of measuring the anti-inflammatory effect.
FIG. 3 is a graph showing the result of measuring sebum secretion inhibiting effect.
FIG. 4 is a graph showing the results of EASI score among clinical test results for patients with atopic dermatitis.
FIG. 5 is a graph showing the results of VAS (visual analogue scale) for pruritus among clinical test results for patients with atopic dermatitis.
FIG. 6 is a graph showing VAS (visual analogue scale) results of sleep disorders among clinical test results for patients with atopic dermatitis.
FIG. 7 is a graph showing the results of trans-epidermal water loss (TEWL) among clinical test results for patients with atopic dermatitis.
FIG. 8 is a graph showing an IGA (Investigator's global assessment) result of a clinical test result for a patient with atopic dermatitis.
FIG. 9 is a photograph showing the clinical course progression in patients with atopic dermatitis before and after use.
FIG. 10 is a photograph showing the clinical course progression in patients with acne dermatitis before and after use.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1: Preparation of plant extracts

Prunella vulgaris leaves and stems, Magnolia liliflora buds, Glycine soya seeds, Glycyrrhiza glabra roots, Centella asiatica leaves and stems are thoroughly cleaned to remove impurities and impurities And shredded at 20 to 25 캜, followed by pulverization so that the particle size was 1 mm or less. 100 g of each of the pulverized powders was immersed in 10 L of 70% ethanol and ultrasonically extracted twice for 24 hours. The extract was filtered through a filter paper (Advantes, No. 2) and concentrated under reduced pressure to prepare respective plant extracts.

Test Example 1: Determination of maximum solubility of plant extract

First, 10 g of each plant extract was dispersed in 90 g of propanediol and dissolved in a constant temperature water bath at 80 ° C for 2 hours in order to confirm the maximum solubility of each plant extract of the present invention in propanediol. As a result, it was confirmed that the centilla sp. Extract powder was completely dissolved in propanediol, but the extracts of Lamiaceae, Lycopersicon esculentum, Pinus densiflora and Licorice extract remained in the form of a precipitate without being dissolved partially. To determine the dry weight of the remaining insoluble extracts, each extract solution was filtered through 400 mesh filter cloth, dried and dried, and the weight of the remaining amount was measured to calculate the content of each extract dissolved in propanediol. Based on this, The maximum solubility that each plant extract can dissolve in propanediol was estimated. The results are shown in Table 1 below.

division Kinds Content (g) Propanediol (g) Solubility Remaining amount (g) Maximum solubility (%) Sample 1 Lamiaceae Extract 10 90 Incomplete melting 4.2 5.8 Sample 2 Seedlings extract 10 90 Incomplete melting 8.8 1.2 Sample 3 Dolch Extract 10 90 Incomplete melting 7.4 2.6 Sample 4 Licorice extract 10 90 Incomplete melting 4.4 5.6 Sample 5 Centipede extract 10 90 Completely dissolved - ?? 10.0

Example 2: Preparation of extraction mixture

The five types of plant extract powders of Test Example 1 were mixed at a predetermined ratio in 10 ratios on the basis of the maximum solubility of each of the plant extracts obtained in Test Example 1 (Samples 1 to 5), dissolved in propanediol, 15 was prepared. For dissolving method, each plant extract powder was firstly mixed at a constant rate at room temperature, and propane diol was added thereto so as to have a total weight of 100 g and dispersed evenly, followed by heating for 2 hours in an 80 ° C water bath to confirm complete solubilization . The results are shown in Table 2 below.

division A (g) B (g) C (g) D (g) E (g) P (g) Dissolution Sample 6 7.0 3.0 5.0 7.0 10.0 68.0 Incomplete melting Sample 7 6.0 2.0 4.0 6.0 10.0 72.0 Incomplete melting Sample 8 5.8 1.5 2.6 5.6 10.0 74.5 Incomplete melting Sample 9 5.8 1.2 2.6 5.6 10.0 74.8 Completely dissolved Sample 10 5.5 1.0 2.5 5.5 10.0 75.5 Completely dissolved Sample 11 5.5 1.5 2.5 5.5 10.0 75.0 Incomplete melting Sample 12 5.0 1.5 2.5 5.0 10.0 76.0 Incomplete melting Sample 13 5.0 1.2 2.5 5.0 10.0 76.3 Completely dissolved Sample 14 5.0 1.0 2.0 5.0 10.0 77.0 Completely dissolved Sample 15 5.0 0.5 2.0 5.0 10.0 77.5 Completely dissolved

 A, Lamiaceae extract; B, Shin extract; C, Dolomoe extract; D, licorice extract; E, centipede extract; P, propanediol

Test Example 2: Confirmation of precipitation phenomenon of the extraction mixture

Samples 9, 10 and 13 to 15, which were completely solubilized solutions of Samples 6 to 15 prepared according to Example 1, were observed at room temperature for 1 day, 10 days, 20 days, 30 days and 60 days, Respectively. Table 3 shows the results of precipitation or precipitation with time.

Test Example 2 division One day past 10 days past 20 days past 30 days past 60 days past Test Example 2-1 Sample 9 - + + + + Test Example 2-2 Sample 10 - - - + + Test Example 2-3 Sample 13 - + + + + Test Example 2-4 Sample 14 - - - + + Test Example 2-5 Sample 15 - - - - -

 +, Precipitated; -, no precipitation

Based on the results shown in Table 3 above, the mixing ratio of each plant extract in Sample 15 was determined as the optimum condition for preparing the extract mixture according to the present invention.

Comparative Example 1: Preparation of each plant extract

In order to verify the efficacy of the extract mixture (sample 15) according to the present invention, Samples 16 to 20 containing only the respective plant extracts as comparative examples were prepared. In particular, in order to confirm whether or not the extract mixture according to the present invention has improved antibacterial, antioxidant, anti-inflammatory and anti-sebum secretion inhibiting effects, Samples 16 to 20 containing only each plant extract (Samples 16 to 20) Sample 15) was prepared as follows.

Samples 16 to 20 were prepared in the same manner as the content of each plant used in the preparation of Sample 15, using propanediol as a solvent. Namely, 5.0 g of Laminariae Radix extract, 0.5 g of Seedlings Extract, 2.0 g of Dolomoe Extract, 5.0 g of licorice extract and 10.0 g of Centella asiatica were prepared as shown in Table 4 below.

sample division Plant extract content (g) Propanediol content (g) Sample 16 Lamiaceae Extract 5.0 95.0 Sample 17 Shin extract 0.5 99.5 Sample 18 Dolch Extract 2.0 98.0 Sample 19 Licorice extract 5.0 95.0 Sample 20 Centipede extract 10.0 90.0

Test Example 3: Measurement of antibacterial effect

As the main causative agents that cause acne dermatitis is propynyl sludge tumefaciens arc Ness (Propionibacterium acnes) is well known, Staphylococcus aureus (Staphylococous) or intestinal bacteria and Enterobacter (Enterobacter) belonging to the like or a complex of these bacteria with other pathogens It has also been reported that it is often caused by infection. In addition, since there is a risk of secondary infection of pathogenic bacteria through damaged skin due to acne dermatitis or atopic dermatitis, antimicrobial efficacy is a very important factor for the treatment or improvement of atopic dermatitis or acne dermatitis.

Acne bacteria In this Test Example 3 (Propionibacterium acnes, KCTC 3314) , Staphylococcus aureus (Staphylococcus aureus, KCTC 1916), E. coli (Escherichia coli, KCTC 2571), the black fungus (Aspergillus niger, ATCC 16404), yeast (Candida albicans , KCTC 7965). Antimicrobial activity of the samples 15 to 20 was measured using a paper disk diffusion method.

First, the strain was cultured in a RC (Reinforced clostridial) liquid medium for 3 days, 100 μl of the suspension was inoculated on an RC agarose plate and adjusted to have an absorbance value of 0.5 at a wavelength of λ620 nm . Paper disks (8 mm, Adventec, USA) were placed on the surface of the plate culture medium inoculated with the strains and 50 μl of each of the samples 15 to 20 was absorbed. As a negative control, 50 μl of propanediol was used, and as a positive control, 50 μl of 1.0% triclosan was used. After the plate medium treated with the sample was incubated for 3 days in an anaerobic jar using a gas pack system, the antimicrobial activity on the basis of the size of the growth inhibition zone around the paper disk Were measured. The size of the growth inhibition ring was measured by dividing the sum of the longest region and the shortest region as the base point of the distal portion of the paper disk. The size of the growth inhibition ring on each test result is as shown in Table 5 below.

Strain Growth inhibition size (mm) Propanediol Triclosan Sample 15 Sample 16 Sample 17 Sample 18 Sample 19 Sample 20 P. acnes - 12.5 12.0 6.0 4.5 - - - S. aureus - 14.0 12.5 7.5 5.0 - - - E. coli - 15.5 9.5 6.5 4.0 - 1.0 - A. niger - 11.0 1.5 1.5 1.0 - - - C. albicans - 9.0 - - - - - -

Referring to the antimicrobial activity results according to the disk diffusion method, in the case of the Lamiaceae extract sample 16 and sample 17 jamokryeon extract acne some degree of antimicrobial to the bacteria (P. acnes), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli) ( A. niger ) and showed no antimicrobial efficacy against C. albicans . In addition, the antimicrobial activity of C. albicans was found to be very effective against A. niger . In the case of the sample 15 as an extract mixture according to the present invention, 1.5 to 2.0 times or more of P. acnes , S. aureus , and E. coli were added to the sample 16 or the sample 17, It has been confirmed that it has superior antibacterial activity. Therefore, it was confirmed that the extract mixture (sample 15) according to the present invention had stronger antimicrobial activity than the single plant extracts (Samples 16 to 20), and compared with the antibacterial activity of triclosan, It is anticipated that it will have excellent efficacy in the treatment and improvement of atopic and acne dermatitis.

Test Example 4: Measurement of antioxidative effect

About 90% of modern human diseases are known to be associated with active oxygen, and excessive active oxygen may be the main cause of atopic dermatitis. Therefore, in order to alleviate atopic dermatitis, it is necessary to inhibit active oxygen, and in order to suppress active oxygen, a substance having excellent antioxidant ability is required. In addition, the antioxidant effect is a major contributor to the reduction of the secretion of inflammatory substances, which is the main cause of acne, thereby helping to alleviate acne dermatitis. Therefore, in Example 4, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging assay was performed to confirm the antioxidative activity of the extract mixture according to the present invention. DPPH is a compound that is decolorized by a proton radical scavenger, and has the advantage of visually confirming the antioxidant activity.

In Experimental Example 4, Sample 15, which is an extract mixture according to the present invention, was used for each dose (10, 20, 50, 100 and 200 μl). After reacting each sample in 2 ml of 0.2 mM DPPH solution at room temperature for 10 minutes, the free radical scavenging ratio (%) was determined by measuring the absorbance at? 520 nm. As a positive control, 50 ㎍ / mL of vitamin C and 50 ㎍ / mL of Trolox were used. The test results are shown in Fig.

As a result of measuring the free radical scavenging ratio (%) of the sample 15, it was found that the antioxidative effect was increased in a concentration dependent manner in the sample 15 as shown in FIG. 1, and the antioxidant activity of vitamin C and trolox It can be seen that they appear to be almost similar to each other.

Test Example 5: Measurement of anti-inflammatory effect

Acne dermatitis is a major cause of inflammation in addition to acne bacteria, and atopic dermatitis is caused by uncontrollable scratching of the skin due to pruritus caused by pruritus, thereby exacerbating inflammation. Thus, substances that have both anti-inflammatory and anti-inflammatory properties may be even more effective in treating or ameliorating acne or atopic dermatitis.

The keratinocytes were transiently transfected with NF-κB (luciferase reporter plasmid) using transfection reagent, followed by microbial toxin ) LPS (Lipopolysaccharide) treatment promotes the production of inflammatory cytokines such as IL-8 (Interleukin 8) and TNF-α (Tumor necrosis factor α), which activate the NF-κB promoter . Therefore, a substance capable of inhibiting the activity of LPS-induced NF-kB promoter can improve acne and atopic symptoms through anti-inflammatory action.

In Test Example 5, the NF-κB inhibition test was performed as follows using the above-described principle. First, NF-κB promoter Gaussia luciferase vector was transfected into 96-well plate of RAW264.7 mouse macrophage cell line using a transfection reagent (Superfect, Qiagen, Germany) Lt; / RTI > Twenty-four hours after the transfection, 50 μl and 100 μl of each of the single plant extract solution samples 16 to 20 and the extract mixture sample 15 of the present invention were respectively treated in the 96-well plate and cultured in a 37 ° C. incubator for 2 hours Respectively. After completion of the pretreatment, 100 ㎕ of LPS at a concentration of 0.5 / / ml was added and cultured in a 37 캜 incubator for 18 hours. The cell culture was collected and reacted with a Gaussian luciferase substrate (NEB, USA), and the luminescence was measured using a Luminescence microplate reader (Infinite 200 Pro, Tecan, Swiss). The experimental results are shown in Fig.

The results of inhibition of LPS-induced NF-kB promoter activity showed that the four plant extracts (Samples 16, 17, 19 and 20), except for the extract of Dolchyong (sample 18) And the anti - inflammatory effect was increased in proportion to the treatment concentration. It can be seen that the extract mixture according to the present invention (sample 15) has a higher anti-inflammatory activity than the single plant extracts, which shows that the extract mixture according to the invention (sample 15) Effect.

Test Example 6: Effect of suppressing sebum secretion

The excessive secretion of male hormones causes sebaceous hyperplasia of sebaceous cells, which causes clogging of the pores, thereby promoting the proliferation of anaerobic bacteria, acne bacteria. Excessive proliferation of acne due to closure of the pores causes inflammation of the skin and eventually causes dermatitis. Therefore, substances capable of inhibiting excessive sebum secretion of sebaceous cells can play an important role in the treatment and improvement of acne dermatitis because they can prevent the clogging of the pores and prevent the proliferation of acne bacteria in a series. .

In order to measure the inhibitory effect of the extracted mixture sample 15 and the comparative samples 16 to 20 on the secretion of sebum secretion, sebaceous gland secretion inhibition was measured by the degree of lipid droplet formation inhibition of Sebocyte. Sebum cells were cultured in DMEM / Ham's F-12 (1: 1) supplemented with 10% fetal bovine serum, and a 96-well plate was coated with 5 x 10 ³ Cells were seeded and further cultured for 2 days. Hydrogen cholesterol was treated with 200 nM of hydroxycholesterol to induce sebum production. Then, 50 쨉 l and 100 쨉 l of each of Samples 15 to 20 were further treated with 100 쨉 l of each of them for 2 days. After washing with PBS (phosphate buffer saline), the lipids induced in the sebaceous cells were stained with Nile red reagent, and then subjected to excitation λ485 using a multifunctional enzyme immunoassay (HTS Multi label reader, Perkin Elmer) nm, Emission The fluorescence intensity at < RTI ID = 0.0 > 555 nm < / RTI > The fluorescence intensity is proportional to the sebum production amount, and the test results are shown in Fig.

As shown in FIG. 3, the samples 16 to 20 showed inhibition of sebum production by the crude extract (sample 17), the black rot extract (sample 18) and the licorice extract (sample 19) The inhibition of sebum secretion was found to be increased. Especially, it showed the best ability to inhibit the sebum production in the extract of Dolch. Lamiaceae extract (Sample 16) and Centella asiatica (Centella asiatica) extract (Sample 20) showed no inhibitory effect on sebum production. On the other hand, the extract mixture (Sample 15) according to the present invention showed stronger sebum production inhibitory ability than any single plant extract. Thus, the ability to inhibit sebum production in sebaceous cells means that it has an excellent effect in the treatment or improvement of acne dermatitis.

Test Example 7: Improvement of dermatitis on atopic patients

(Example 1 to 4) using the extract mixture (sample 15) according to the present invention, and then the skin condition after use was measured by EASI (Eczema area and severity index scale, VAS (visual analogue scale), TEWL (transepidermal water loss), and IGA (Investigator's global assessment).

Forty - five patients were selected first, but five of them withdrew from the trial by voluntarily, and 40 patients were tested for atopic dermatitis. The control group consisted of 19 patients and the test group consisted of 21 patients. In the control group, a lotion prepared using propanediol instead of the mixed-extract solution mixture according to the present invention was used in formulation example 1-4, and a lotion made from the extract mixture (sample 15) according to the present invention was used in the test group. The information of the subjects participating in the test is shown in Table 6 below.

division Total staff (40) The control group (19 patients) The test group (21 patients) Age (years) 20.2 ± 17.8 19.6 ± 17.4 20.4 ± 17.6 gender male 23 (57.5%) 10 (52.6%) 13 (61.9%) female 17 (42.5%) 9 (47.4%) 8 (38.1%) EASI score 10.1 ± 5.1 9.6 ± 4.0 10.6 ± 6.0

The Friedman test was applied to the repeated measures and statistical analysis was carried out to confirm the difference between the time points of the variables with significant results. Wilcoxon's signed rank test was used for the statistical significance test of pairwise continuous variables such as time difference within each group of variables and difference of variation between groups of variables. All statistical analyzes were performed using SPSS for windows (Version 18, IBM, New York, NY, USA) as statistical software. For better understanding, the test results are shown graphically in Figs. 4-8.

division Time of evaluation Post-comparison ( p value) Point Two weeks 4 weeks Against time
2 weeks Against time
4 weeks Two weeks
4 weeks I'm
sieve
(40) EASI score 10.1 ± 5.1 9.7 ± 5.1 9.1 ± 5.2 0.064 0.065 0.054 VAS Pruritus 4.4 ± 1.8 3.8 ± 1.8 3.8 ± 2.2 0.011 0.058 0.968 Sleep disorder 0.8 ± 1.0 0.5 ± 0.7 0.3 ± 0.5 0.000 0.001 0.073 TEWL (arm) 21.4 ± 12.4 16.6 + - 10.4 17.8 ± 11.4 0.002 0.025 0.341 IGA 1.8 ± 0.7 1.5 ± 0.6 1.4 ± 0.7 0.003 0.002 0.210 versus
article
group
(19) EASI score 9.6 ± 4.0 9.6 ± 4.0 9.4 ± 4.7 0.909 0.728 0.648 VAS Pruritus 4.4 ± 1.7 3.7 ± 2.0 3.8 ± 2.1 0.027 0.094 0.672 Sleep disorder 1.1 ± 1.1 0.6 ± 0.8 0.3 ± 0.7 0.000 0.007 0.172 TEWL (arm) 24.0 ± 13.8 19.4 ± 12.5 20.9 ± 13.8 0.036 0.235 0.482 IGA 1.6 ± 0.7 1.5 ± 0.6 1.5 ± 0.7 0.667 0.542 0.667 city
Hum
group
(21) EASI score 10.6 ± 6.0 9.8 ± 6.0 8.9 ± 5.8 0.012 0.004 0.017 VAS Pruritus 4.4 ± 2.0 3.8 ± 1.6 3.7 ± 2.3 0.143 0.232 0.853 Sleep disorder 0.6 ± 0.7 0.3 ± 0.6 0.2 ± 0.4 0.000 0.042 0.267 TEWL (arm) 18.5 ± 10.1 14.6 ± 6.6 14.2 ± 6.4 0.022 0.021 0.525 IGA 1.9 ± 0.7 1.4 ± 0.7 1.3 ± 0.6 0.000 0.000 0.186

First, referring to Table 7 and FIG. 4, the EASI score of the control group was decreased with time (2 weeks, 4 weeks) compared to the time point, but statistically . On the other hand, the EASI score of the test group showed a statistically significant decrease over time (2 weeks, 4 weeks) compared to the time point. EASI score reductions at 2 and 4 weeks compared to baseline were greater in the test group compared to the control group and the EASI score reduction between 2 and 4 weeks was also greater in the test group. A significant decrease in EASI scores means that the severity of clinical symptoms of atopic dermatitis has decreased.

FIG. 5 is a graph showing evaluation of the degree of reduction of pruritus by VAS (visual analogue scale). In the case of pruritus, the visual scale score of the control group was decreased at 2 weeks compared to the time point, , But decreased at the point of time. Visual scoring scores for pruritus in the test group tended to decrease statistically with time (2 weeks, 4 weeks) compared to the time point. The reduction of the visual scoring score for pruritus compared to the time point was greater in the control group at 2 weeks, and larger in the test group at 4 weeks. Between 2 and 4 weeks, the control group showed a rather increased visual score score for pruritus, whereas it decreased in the test group.

FIG. 6 is a graph showing an evaluation of the degree of decrease in sleeping disturbance by VAS (visual analogue scale). The visual scoring score was statistically significant at 2 and 4 weeks according to time in both the control group and the test group Respectively.

FIG. 7 is a graph showing the measurement of trans-epidermal water loss (TEWL). In the case of the control group, it decreased at 2 weeks, but increased at 4 weeks rather than at 2 weeks. When compared, the transdermal water loss decreased. The test group showed a statistically significant decrease at 2 weeks as time elapsed compared with the time point, but the decrease was less than 2 weeks at 4 weeks, but it was not statistically significant. The transdermal water loss was statistically significantly decreased in the control and test groups at 2 weeks, but decreased significantly at 4 weeks compared with the control group. A decrease in transdermal water loss indicates an increase in skin moisturizing power, thus showing a good moisturizing effect.

FIG. 8 shows the results of the IGA (Investigator's global assessment) score by the researcher, but the control group was decreased at 2 weeks and 4 weeks as time elapsed, but there was no statistical significance. The test group showed statistically significant decrease at 2 and 4 weeks according to the time.

FIG. 9 is a photograph showing a comparison of the mitigation or improvement effect of atopic dermatitis patients in the test group over time, showing a significant improvement in the first two weeks after use, though it varies depending on the patient In some cases, improvements were seen in 3 or 4 weeks.

Test Example 8: Improvement of dermatitis on acne patients

In Test Example 8, a serum was prepared according to the following Preparation Example 1-3 using the extract mixture (Sample 15) according to the present invention, and then an efficacy clinical test was conducted on acne dermatitis. Because there were not enough subjects, clinical trials were conducted only with the test group, and the control group was not tested separately. A total of 12 subjects were divided into 8 oars and 4 dryers depending on the type of skin, and they were divided into 4 acne areas, which were mainly forehead, chin, And whether treatment or amelioration of dermatitis was tested. The results of the clinical tests are shown in Table 8 and FIG. 10 below.

Subject Age / Sex / Skin Type Types of Acne Dermatitis Rate of improvement (%) One 17 years old / male / intelligence Papule + pustule + nodule 80 2 17 years old / female / intelligence Papule + pustule 70 3 18 years old / male / intelligence papule 70 4 18 years old / female / intelligence papule 80 5 19 year old / male / dry Papule + pustule + nodule 60 6 20 years old / woman / intelligence papule 90 7 21 year old / female / dry Papule + pustule 70 8 23 years old / woman / intelligence papule 80 9 24 year old / male / dry papule 70 10 24 years old / female / intelligence Papule + pustule 40 11 26 years old / female / dry papule 70 12 27 years old / female / intelligence papule 80

 1) Pumice: The acne bacterium propagates, the inflammation reaction starts and erythema starts, and the red inflamed skin

 2) Parenchyma: A small bump with inflammation and leukocyte accumulation composed of a mixture of inflammatory cells and liquid

 3) Nodule: It is the same shape as the papule, but the papillary acne progresses and the pustule expands to the surrounding tissues and deep tissues in the skin, and its diameter is formed to be larger or deeper about 5 ~ 10 mm

As shown in Table 8, when the serum containing the extract mixture according to the present invention was applied to the affected area for 4 weeks, the overall improvement of the acne dermatitis in most patients regardless of the skin type was observed in 12 acne dermatitis patients Effect. However, in the case of 24-year-old women with oily skin, the degree of improvement was not prominent, but it was found to be improved.

As can be seen from FIG. 10, the skin condition improved overall regardless of the type of dermatitis diseases such as papules, pustules, nodules, and the like, and it was found to have excellent effects especially on papules.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or essential characteristics thereof, , Test examples, and formulations are to be considered in an illustrative manner to facilitate understanding of the invention rather than a limited perspective. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof are to be construed as being included in the present invention.

Formulation Example 1: Cosmetic preparation

1-1. Wash

ingredient content (%) The extraction mixture (sample 15) 3.0 Dysodium cocoamphodiacetate 20.0 Lauryl glucoside 10.0 Sodium lauroyl glutamate 10.0 Cydric Acid 2.0 antiseptic Suitable amount Spices Suitable amount Purified water Balance system 100

After mixing the above components, they were prepared according to the usual production method.

1-2. skin

ingredient content (%) The extraction mixture (sample 15) 3.0 Panthenol 0.5 Trehalose 1.0 glycerin 2.0 Betaine 2.0 Hyaluronic Acid 0.01 Xanthan gum 0.05 Aloe vera leaf extract 0.05 antiseptic Suitable amount Spices Suitable amount Purified water Balance system 100

After mixing the above components, they were prepared according to the usual skin preparation method.

1-3. Serum

ingredient content (%) The extraction mixture (sample 15) 5.0 Panthenol 0.5 Sorbitol 1.0 glycerin 2.0 Betaine 2.0 Carbomer 0.5 Xanthan gum 0.2 Hyaluronic Acid 0.1 antiseptic Suitable amount Spices Suitable amount Purified water Balance system 100

After mixing the above components, they were prepared according to the usual skin preparation method.

1-4. Lotion

ingredient content (%) The extraction mixture (sample 15) 5.0 Panthenol 0.5 glycerin 1.0 Betaine 2.0 Carbomer 0.5 Xanthan gum 0.2 Hyaluronic Acid 0.1 lecithin 1.0 Beads wax 2.0 Sheer butter 1.0 Caprylic / capric triglyceride 4.0 antiseptic Suitable amount Spices Suitable amount Purified water Balance system 100

1-5. cream

ingredient content (%) The extraction mixture (sample 15) 5.0 Panthenol 0.5 glycerin 1.0 Betaine 2.0 Hydroxyethanol 1.0 Carbomer 0.5 Xanthan gum 0.2 Hyaluronic Acid 0.05 lecithin 1.5 Beads wax 3.0 Sheer butter 2.0 Caprylic / capric triglyceride 5.0 antiseptic Suitable amount Spices Suitable amount Purified water Balance system 100

After mixing the above components, they were prepared according to the usual cream production method.

Formulation Example 2: Preparation of a pharmaceutical preparation

2-1. Ointment

ingredient Content (g) The extraction mixture (sample 15) 10.0 Olive oil 14.5 beeswax 3.7 1,3-butylene glycol 6.3 P-hydroxybenzoic acid profile 0.1 Methyl paraoxybenzoate 0.2 Hydrolyzed fibroin 2.0 water 15

The above components were mixed and stirred with a stirrer to prepare an ointment.

2-2. cream

ingredient Content (g) The extraction mixture (sample 15) 5 Stearic acid 1.5 Stearyl alcohol 2.2 Butyl stearate 0.5 Propylene glycol 0.5 Glycerin monostearate 2.0 Potassium hydroxide 0.3 Cream base 40

After mixing the above components, the cream was prepared by degassing, filtering and cooling.

Claims (7)

A cosmetic composition comprising, as an active ingredient, an extract mixture of Prunella vulgaris , Magnolia liliflora , Glycine soya , Glycyrrhiza glabra and Centella asiatica , wherein the cosmetic composition is antibacterial, Antioxidant, suppression of secretion of sebum, and improvement of dermatitis. The extract of claim 1, wherein the extract mixture comprises 50-150 parts by weight of a crude extract, 50-150 parts by weight of a spinach extract, 50-150 parts by weight of a licorice extract, and 50-150 parts by weight of a centiporepenus extract, based on 100 parts by weight of an extract of Lamiaceae Wherein the cosmetic composition is a cosmetic composition. [Claim 3] The cosmetic composition according to claim 2, wherein the extract mixture is prepared by mixing Lamiaceae L., Seedlings Extract, Dolchy Extract, Licorice Extract and Centella asiatica Extract with a hydrophilic solvent. 4. The composition according to any one of claims 1 to 3, wherein the composition is selected from the group consisting of a mist, a skin, a lotion, an essence, a cream, a chestnut, a pack, a powder, a foundation, a foaming cleanser, a body wax, a shampoo, ≪ / RTI > A pharmaceutical composition comprising, as an active ingredient, an extract mixture of Prunella vulgaris , Magnolia liliflora , Glycine soya , Glycyrrhiza glabra and Centella asiatica , wherein the cosmetic composition is an antibacterial, , Antioxidation, suppression of sebum secretion, and improvement of dermatitis. [Claim 5] The method according to claim 5, wherein the extract mixture comprises 50-150 parts by weight of a crude extract, 50-150 parts by weight of a spinach extract, 50-150 parts by weight of a licorice extract, and 50-150 parts by weight of a centurion extract, ≪ / RTI > [Claim 6] The pharmaceutical composition according to claim 5, wherein the extract mixture is prepared by mixing Lamiaceae L., Lycoris spp. Extract, Lycopersicon esculentum extract, Licorice root extract and Centella asiatica L. extract in a hydrophilic solvent.

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