KR20160061641A - Smear device of cytology for making microscope slides and the smearing method thereof - Google Patents

Abstract

The present invention relates to a cytology screening smearing device for manufacturing a microscope slide, and a cell smearing method using the same. The cytology screening smearing device has a substantially improved effect of preventing errors in smearing slides by comprising a valve body moving to the left or right side of a backdraft prevention means to prevent a back flow of a solution liquid. The cytology screening smearing device automatically and conveniently performs separating solution liquid and cells, and smearing slides; and allows cells to be uniformly seated by constituting an edge of a filter and a mesh at a center part to be different from each other. The smearing device comprises: a main body (100); a filter part (200); a suctioning and discharging part (300); a smearing part (400); and a storage container (500).

Description

TECHNICAL FIELD The present invention relates to a smear device for smearing cells for microscope slides and a method for smearing cells using the same,

The present invention relates to an apparatus for smearing a test cell for producing a microscope slide and a method for smearing cells using the same, and more particularly, to a method and apparatus for automatically and easily separating a solution and a cell, And the center part mesh are configured to be different from each other to allow the cells to be uniformly positioned, and the valve body is moved to the left or right by the backflow preventing means to prevent the backflow of the solution solution. Thus, And a method for smearing cells using the same.

Generally, in order to examine the presence or absence of a physical abnormality, there is used a method of collecting cells at the site, storing the cells in a solution container containing a solution solution of the liquid, attaching the liquid cells to the slide, and inspecting them with a microscope.

Traditionally, the process of transferring cells from a solution bottle to a slide has been largely manual by the user.

According to this method, after having a separate solution bottle for dispersing the cells on the brush into the solution, shake the solution bottle, pour the solution into another container while the cells are sufficiently spread in the solution, When the container is separated from the main body so as to approach the slide, the mixture remaining in the container is poured down by gravity and is lost. .

However, since the process of transferring the cells from the solution bottle to the slide must be performed manually by the user, it takes a lot of time and the pollution or impurities may be transferred from the user's hand to the slide, .

In addition, foreign substances may be contained in cells other than the cells collected in the solution container. In this case, it is not easy to separate the foreign substances from the cells.

In addition, as described above, the direct smearing method has a disadvantage in that it is difficult to detect and accurately read cancer cells because superimposition phenomena in which cells overlap can occur.

The following is a typical prior art related to a smearing apparatus having a simple structure for easily separating liquid cells and solution solution to solve the above problems.

Korean Patent Nos. 10-1405712 and 10-1336494 are filed by the applicant of the present application and are directed to an apparatus for smearing liquid cell, which is formed into a cylindrical shape and contains a solution solution for smearing, A filter vessel; A substrate on which a filter fixing part is installed at a predetermined height at a bottom part and a charging part for charging a membrane filter container is installed on an upper surface of the filter fixing part; A lifting means installed between the substrate and the filter fixing portion and lifting the lifting plate between the bottom portion of the substrate and the filter fixing portion so as to facilitate separation of the upper and lower portions of the filter container coupled to the charging portion; And a slide glass forward / backward movement means for moving the slide glass, which is seated on the seatback portion, forward and backward to the lower end of the filter fixing portion, and; A suction cylinder installed at the other side of the filter fixing part of the substrate and connected to the lifting plate of the lifting unit by a hose is provided and a valve is provided between the suction cylinder and the hose to control the suction amount of the solution solution, And a suction and drainage means for discharging the solution solution sucked into the suction cylinder.

However, in the apparatus for smelting liquid cells as described above, since the filter of the membrane filter container has the same mesh at the edge and the center, when the pressure is applied at the lower part for suction of the solution solution, the cells are concentrated to the center, There is a problem that the valve has a problem that the backflow prevention effect is lowered when the solution force absorbing and discharging pressure lowers the clamping force of the blocking plate.

Korean Patent No. 10-1405712 (2014.06.02.) Korean Patent No. 10-1336494 (November 27, 2013) Korean Patent No. 10-1058409 (Aug. 16, 2011)

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the problems of the prior art as described above, and it is an object of the present invention to provide a method of automatically separating a solution solution and a cell, And a valve body for preventing backflow of the solution solution by moving the valve body to the left or right by the backflow preventing means and preventing the reverse flow of the solution solution, And a method of smearing cells using the same.

According to an aspect of the present invention, And an inlet for inserting a membrane filter container in which a cell and a solution solution are contained is formed in a portion of the main body and protruded to an upper portion of the main body, A filter unit provided with an elevator that moves up and down to facilitate lower separation; And a cylinder connected to the filter unit by a hose for sucking solution solution in the membrane filter container and discharging the solution solution to the outside is provided. The driving force of the cylinder, which is provided at one side of the cylinder, A suction port provided on one side of the hose for adjusting the suction amount of the solution solution under the control of the control unit and provided with a check valve for preventing the reverse flow when sucking and discharging the solution solution, A discharge portion; A stepping motor for generating a driving force such that the seat is moved forward and backward to the inside of the filter unit is provided on one side of the seat glass, A smear unit provided with the smear means; And a reservoir which is located below the smear unit and has a space therein and in which the smear glass is dropped and stored in the smear unit to prevent drying of the smear cell,

The membrane filter container is formed with a receiving port having an upper opening to receive a solution solution containing cells, a lower opening of the receiving port is formed, and an opening is formed between the receiving port and the opening A filter having a mesh number larger than that of the membrane so that the cells passing through the membrane are seated on the upper surface of the filter, And a lower filter body disposed at a lower portion of the filter, the lower filter body having a drain port through which the solution solution is drained,

The backflow prevention valve includes a block, a DC motor provided at one side of the block, and a double-acting valve coupled to the DC motor, A joint cap which is coupled to the lower portion of the cam plate and moves to the left or right side in the same manner as the lower direction of the cam plate, and a joint cap which is coupled to both sides of the joint cap, A space is formed in the center of the part coupled with the block and moved to the left or right so as to be the same as the direction of movement of the joint cap to control opening and closing of a plurality of through holes formed in the block, And a valve body.

The apparatus for smearing a test cell for producing a microscope slide according to the present invention and the method for smearing cells using the same can automate the process of separating solution and cells and the entire process of slide smear, Time efficiency can be increased.

In addition, as the work process is automated, the smearing operation of the cells is performed quickly without external contamination, thereby improving the accuracy of the inspection.

In addition, the edges of the filter constituting the membrane filter container and the meshes of the central portion are made different from each other, so that the cells are uniformly seated, thereby remarkably enhancing the effect of preventing the slide smearing failure.

In addition, the valve body constituting the check valve is moved to the left or right to prevent backflow of the solution solution, thereby improving the problem of the conventional blocking plate and significantly improving the slide smearing prevention effect.

In addition, since the stepping motor is provided, the pressure can be precisely controlled so that the cells can be uniformly placed.

In addition, the membrane filter container is rotated and fixed 90 ° clockwise after the user inserts the membrane filter container. However, according to the present invention, when the membrane filter container is inserted, the holder automatically fixes the membrane filter container, There is an effect that it can be promoted.

When the membrane filter container is separated into upper and lower parts, the upper and lower filter main bodies are separated into an upper filter main body and a lower filter main body. At the same time, the upper filter main body is automatically removed, So that the convenience of the user can be improved.

In addition, conventionally, the slide glass which has been smeared in the smearing part is put in a 95% alcohol bottle directly by the user to prevent the drying of the cells until the diagnosis of the microscope, so that the user must wait until the blotting is completed and then transfer it to the alcohol bottle. , There is a problem that when the transferring process is delayed, the cell is dried due to a long exposure time in the air. In the present invention, since the slide glass, which has been smear-finished, is automatically dropped into the storage container containing 95% alcohol by the provision of the storage tube, it is not troublesome and the preservation ability of the cells can be enhanced.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view showing a conventional smelting apparatus for liquid cells. FIG.
FIG. 2 is a perspective view showing an apparatus for smearing test cells for producing a microscope slide according to an embodiment of the present invention; FIG.
3 is a left side view showing a device for smearing test cells for producing a microscope slide according to an embodiment of the present invention.
FIG. 4 is an internal structural view showing a device for smearing test cells for producing a microscope slide according to an embodiment of the present invention. FIG.
FIG. 5 is an internal structural view showing a suction and discharge unit of a test cell smear apparatus for producing a microscope slide according to an embodiment of the present invention; FIG.
FIG. 6 is a view showing a check valve of a smearing apparatus for a test cell for producing a microscope slide according to an embodiment of the present invention. FIG.
7 is a view showing a membrane filter container of a smearing apparatus for a test cell for producing a microscope slide according to an embodiment of the present invention;

Hereinafter, preferred embodiments of the present invention will be described in detail. These examples are for further illustrating the present invention, and the scope of rights of the present invention is not limited to these examples.

Referring to the accompanying drawings, a configuration of an embodiment of a test cell smearing apparatus for producing a microscope slide according to the present invention will be described in detail below.

The apparatus for smearing test cells for producing the microscope slides includes a main body 100, a filter unit 200, a suction and discharge unit 300, a smear unit 400, and a storage cylinder 500.

More specifically, the main body 100 has a quadrangular external appearance, and an insertion port 220 for inserting a membrane filter container 210 having cells and a solution solution therein is installed at an upper center thereof. And a switch panel for driving and controlling the display window and the apparatus. The slide glass is mounted on the front surface of the switch panel 410, And a storage cylinder 500 is provided.

In this case, the main body 100 may be made of aluminum, which is excellent in corrosion resistance and is excellent in cold workability as a heat-treated alloy, and the check valve 320 constituting the suction and discharge part 300 has tensile strength, bending strength , Polyacetal (POM) having high impact strength, excellent abrasion resistance, low friction resistance, self-lubricating property, good heat resistance, resistance to weak acid, weak alkali, gasoline, benzene, oil and alcohol and excellent weather resistance The durability of the solution solution to be sucked is high, so that the durability of the check valve 320 can be increased.

The filter unit 200 is provided inside the main body 100 and partly protrudes from the main body 100. The filter unit 200 includes the insertion port 220 for inserting the membrane filter container 210, And an elevator 230 coupled to the insertion port 220 and vertically moving up and down to facilitate separation of the upper and lower portions of the membrane filter container 210 inserted therein.

At this time, the membrane filter container 210 is formed with a receiving opening 212 having an upper opening to receive the solution solution containing the cells, a fitting opening 213 having a lower opening positioned below the receiving opening 212, An upper filter main body 211 formed between the receiving port 212 and the fitting hole 213 to form a membrane 214 for filtering foreign substances larger than the cell size; A filter 216 having a greater mesh number than that of the membrane 214 is formed on the upper surface of the filter 216 so that cells passing through the membrane 214 are seated on the upper surface of the filter 216, And a lower filter body 215 in which a drain port 217 through which the solution liquid that has passed through the filter 216 is drained is formed.

For example, the number of meshes of the membrane 214 may be 150 to 300 meshes, and the mesh of the filter 216 may be 500 to 700 meshes.

In this case, it is needless to say that the filter 216 can be manufactured such that the mesh is more centered than the edge so that the cells are uniformly seated when pressure is applied to suck the solution solution from the bottom. If the mesh is the same at both the edge and the center of the filter 216, the cells are pushed to the center when the pressure is applied at the bottom, resulting in a problem in that the accuracy of cell diagnosis is deteriorated.

In this case, the membrane filter container 210 is conventionally rotated by 90 ° in a clockwise direction after the user inserts the membrane filter container 210. However, the present invention is not limited to this, There is an effect that the convenience of the user can be improved by fixing the membrane filter container 210.

The suction and discharge unit 300 is connected to the filter unit 200 by a hose to install a cylinder 310 for sucking solution solution in the membrane filter container 210 and discharging the solution solution to the outside, A stepping motor provided at one side of the cylinder 310 for adjusting the suction pressure according to the driving force of the cylinder 310 and the control of the control unit, And a backflow prevention valve 320 for preventing backflow when sucking and discharging the solution solution.

At this time, the sucking pressure at the time of sucking the solution solution is 1961 to 2508 Pa. Since the kinds of effective cells are different depending on the specimen, the pressure is adjusted with the stepping motor precisely, Of course. If the suction pressure exceeds 2508 Pa, the number of cells that are ruptured or adsorbed to the filter 216 and adsorbed to the filter 216 may decrease, Is not adsorbed uniformly, so that cells are bundled together and the accuracy of the test may be lowered.

The check valve 320 includes a block 321, a DC motor 322 disposed at one side of the block 321, and a DC motor 322 coupled to the DC motor 322, The cam plate 323 is moved in the left and right directions with its center fixed and the upper and lower directions of the cam plate 323 are different from each other and the cam plate 323 is coupled to the lower portion of the cam plate 323, A joint cap 324 which is connected to both sides of the joint cap 324 and which is formed in a cylindrical shape and narrowed in the center so that a space is formed at the center among the parts joined with the block 321, And a valve body 325 which is moved to the left or right so as to be the same as the movement direction of the joint cap 324 and which controls the opening and closing of a plurality of through holes formed in the block 321 to prevent the solution solution from flowing backward .

The smear unit 400 is disposed on the front surface of the main body 100 and the slide glass is provided with the socket 410 on which the slide glass is mounted for smearing cells. A stepping motor for generating a driving force is installed so that the seat 410 is moved forward and backward to the inside of the filter unit 200.

In addition, the main body 100 is provided with a space below the smear unit 400 and has a space therein. In order to prevent drying of the smear cells, the smear glass that has been smudged by the smear unit 400 drops A storage cylinder 500 is provided.

At this time, conventionally, the slide glass which has been smeared in the smear unit 400 is put in a 95% alcohol bottle directly to prevent the drying of the cells until the diagnosis of the microscope is performed to increase the retention of the slide glass. It is cumbersome to transfer, and if the transferring process is delayed, there is a problem that the cell is dried due to a long exposure time to the air in the cell. In the present invention, since the slide glass having the smear completed is automatically dropped into the storage cylinder 500 containing 95% alcohol by the provision of the storage cylinder 500, it is not troublesome and the preserving force of the cells can be enhanced.

Hereinafter, a method for smearing cells using a smearing apparatus for a test cell for producing a microscope slide will be described below.

First, the membrane filter container 210 containing the cells and the solution solution is inserted into the main body 100 through the insertion port 220.

Next, only the cells inside the inserted membrane filter container 210 are placed on the filter 216, and the stepping motor provided in the suction and discharge unit 300 is driven so that the solution liquid is sucked, And sucks the solution solution through the check valve (320).

At this time, the solution solution containing the cells accommodated in the receiving port 212 of the upper filter body 211 of the membrane filter container 210 is moved downward by the pressure of the cylinder 310, A foreign matter larger than the size of the cell is filtered and moved to the fitting hole 213. Thereafter, impurities and solution liquid passing through the filter 216 of the lower filter body 215 and smaller than the size of the cells excluding the cells are moved to the drain port 217. This causes the cells to settle on the upper surface of the filter 216 of the lower filter body 215. At this time, by using the stepping motor, the suction pressure can be regulated so that the cells can be accurately and uniformly placed.

Next, the sucked solution liquid is discharged to the outside through the discharge port, and the solution solution is discharged through the check valve 320 so as not to flow back to the membrane filter container 210 side at the time of discharge.

The check valve 320 includes a first through hole 321a through which a hose connected to the membrane filter container 210 is attached to the block 321 and a hose or cylinder hose connected to the cylinder 310 And a third through hole 321c to which a hose connected to the discharge port is attached,

The valve body 325 moves to the left so that the first through hole 321a and the second through hole 321b are opened and the third through hole 321c is closed so that the cylinder 310 The solution inside the membrane filter vessel 210 flows through the first through hole 321a between the block 321 of the check valve 320 and the center of the valve body 325 And the introduced solution solution is stored in the cylinder hose through the second through hole 321b,

The valve body 325 moves to the right to open the second through hole 321b and the third through hole 321c and close the first through hole 321a, The piston inside the valve body 310 rises and the solution solution stored in the cylinder hose flows into the space between the block 321 of the check valve 320 and the center of the valve body 325 through the second through hole 321b The solution solution flows into the outlet through the third through-hole 321c and is discharged to the outside. At this time, the first through-hole 321a is closed and does not flow back to the membrane filter container 210 side It is effective.

Also, as described above, since the corresponding through holes are opened and blocked according to the respective operations at the time of suction and discharge, the internal pressure is maintained, so that efficient suction and discharge can be achieved.

Next, when the solution liquid is discharged from the membrane filter container 210 and the cells are placed on the filter 216, the elevator 230 coupled to the lower portion of the insertion port 220 into which the membrane filter container 210 is inserted Is lowered to separate the membrane filter vessel 210 into upper and lower portions.

At this time, when the membrane filter container 210 is separated into the upper and lower portions, the upper and lower filter bodies 211 and 215 are separated and the upper filter body 211 is automatically removed to be.

Next, the slide glass is moved to the upper side of the lower filter body 215 on which the cells are placed. At this time, the slide glass is driven by a stepping motor provided in the smear unit 400, and the seat 410 on which the slide glass is placed is moved.

Next, the elevator 230 is lifted so that the upper side of the lower filter body 215 is brought into contact with the lower surface of the moved slide glass, and the cells are smudged on the lower surface of the slide glass.

Next, when the cells are smudged, the elevator 230 is lowered again and the lower filter body 215 is separated from the slide glass.

In this case, the lower filter body 215 may be automatically removed after being separated from the slide glass.

Next, the smear-completed slide glass is moved to the original position, and then dropped into the storage container 500, and is stored until the slide glass is taken out by the user.

At this time, since 95% alcohol is filled in the preserving cylinder 500, the time for which the cells smear on the slide glass is exposed to the air can be reduced, thereby reducing the drying time of the cells, .

100:
200:
210: Membrane filter container
211: upper filter body
212: Receiver
213:
214:
215: Lower filter body
216: Filter
217: drain
220:
230: Lift
300: suction and discharge unit
310: cylinder
320: Backflow prevention valve
321: Block
321a: first through hole
321b: second through hole
321c: Third through hole
322: DC motor
323: cam plate
324: Joint cap
325: Valve body
400:
410: seat
500: Storage container

Claims (5)

A main body 100;

And an insertion port 220 for inserting a membrane filter container 210 having cells and a solution solution therein and protruding from the upper part of the main body 100 is provided in the main body 100, A filter unit 200 coupled to the membrane filter 220 and having an elevator 230 for moving up and down the membrane filter vessel 210 to facilitate separation of the upper and lower portions of the membrane filter vessel 210;

A cylinder 310 connected to the filter unit 200 through a hose to suck the solution solution in the membrane filter vessel 210 and discharge the solution solution to the outside is installed, A stepping motor for adjusting the suction pressure according to the control of the control unit, and a stepping motor provided on one side of the hose for controlling the suction amount of the solution solution under the control of the control unit, A suction and discharge unit 300 provided with a check valve 320 for preventing a back flow at the time of discharge;

The slide glass is provided with a mounting hole 410 on the front surface of the main body 100 and on which the slide glass is mounted for smearing cells. The mounting hole 410 is formed at one side of the mounting hole 410, A smear unit 400 having a stepping motor for generating a driving force so as to move back and forth inside the unit 200;

A reservoir 500 is disposed below the smear unit 400 and has a space therein to prevent the smearing cells from being dried to allow the slide glass to be smudged from the smear unit 400 to fall down, ; Wherein the cell is a test cell.
The method according to claim 1,
The check valve (320)
Block 321,
A DC motor 322 provided on one side of the block 321,
A cam plate 323 which is coupled with the DC motor 322 and moves left and right in response to the rotation of the DC motor 322 and whose center is fixed and whose upper and lower directions of motion are different,
A joint cap 324 which is coupled to the lower portion of the cam plate 323 and moves to the left or right in the same direction as the lower direction of the cam plate 323,
And a valve body (325) coupled to both sides of the joint cap (324), each of which has a cylindrical shape and a narrow central portion, and a space is formed at a center of the portion coupled with the block (321) Apparatus for smearing cells for inspection.
The method according to claim 1,
The membrane filter vessel (210)
A receiving hole 212 is formed in the upper part of the receiving hole 212 so as to receive the solution solution containing the cells and a lower part of the receiving hole 212 is formed in the lower part of the receiving hole 212, An upper filter body 211 is disposed between the upper filter body 211 and the lower filter body 213 to form a membrane 214 for filtering foreign substances larger than the cell size,
A filter 216 which is detachably coupled to the inside of the fitting hole 213 and has a mesh number larger than that of the membrane 214 is formed on the upper surface so that the cells passing through the membrane 214 are seated, And a lower filter body (215) formed at a lower portion of the lower filter body (215) to form a drain hole (217) through which the solution solution passed through the filter (216) is drained.
Inserting a membrane filter container (210) containing cells and a solution solution into the main body (100) through an insertion port (220);

The stepping motor provided in the suction and discharge unit 300 is driven so that only the cells inside the inserted membrane filter container 210 are seated on the filter 216 and the solution liquid is sucked, Suctioning the solution solution through the prevention valve 320;

Discharging the solution solution through the check valve 320 so that the sucked solution solution is discharged to the outside through the discharge port and does not flow back to the membrane filter container 210 side when discharged;

When the solution liquid is discharged from the membrane filter container 210 and only the cells are seated on the filter 216, the elevator 230 coupled to the lower portion of the insertion port 220 is lowered, Separating the upper part and the lower part into two;

Moving the slide glass by a stepping motor provided on the smear unit 400 on the upper side of the lower filter body 215 on which the cells are placed in the upper and lower membrane filter containers 210;

A step of raising the elevator (230) so that the upper side of the lower filter body (215) is in contact with the lower side of the moved slide glass, and the cells are laid on the lower side of the slide glass;

When the cells are smashed, the elevator 230 is lowered again and the lower filter body 215 is separated from the slide glass;

The smear-completed slide glass is moved to its original position, then falls into the storage cylinder 500 and is stored until the user takes out the slide glass; A method for smearing a cell using a smearing apparatus for a test cell for producing a microscope slide.
5. The method of claim 4,
The check valve (320)
In the step of sucking the solution solution, the valve body 325 moves to the left side and the piston inside the cylinder 310 descends, and the solution solution in the membrane filter vessel 210 flows through the hose to the check valve 320, and the introduced solution liquid is stored in the cylinder hose,
In the step of discharging the solution solution, the valve body 325 moves to the right side and the piston in the cylinder 310 rises. The solution solution stored in the cylinder hose flows into the check valve 320 again, The introduced solution solution is transferred to the discharge port through the hose and discharged to the outside,
Wherein the valve body (325) is controlled to open and close each through hole corresponding to the suction or discharge, thereby preventing the backflow of the solution solution.

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