KR20140132869A - Bio-sensor array for measuring glucose, glycated protein and total albumin - Google Patents

Abstract

The present invention relates to a biosensor array for measuring glucose, glycated protein, and total protein at the same time which comprises: a substrate; a first sensor including a first sensing film formed on the substrate, and glucose oxidase (GOD) which is a glucose receptor combined with glucose, being attached to a first source electrode and a first drain electrode connected with both ends of the first sensing film, and the first sensing film; a second sensor adjacent to the first sensor, and including a second sensing film formed on the substrate, and glycated albumin antibodies being a glycated protein receptor combined with glycated protein, being attached to a second source electrode and a second drain electrode connected with both ends of the second sensing film, and the second sensing film; and a third sensor adjacent to the second sensor, and including a third sensing film formed on the substrate, and albumin antibodies which is a total protein receptor combined with total protein, being attached to a third source electrode and a third drain electrode connected with both ends of the third sensing film, and the third sensing film. The treated blood is applied to the first, second, and third sensors, so the glucose receptor, the glycated protein receptor, and the total protein receptor are combined with the glucose, glycated protein, and total protein existing in the blood. The currents flowing through the first, second, and third sensing films are measured, so the levels of the glucose, glycated protein, and total protein are measured at the same time. Moreover, the present invention relates to a method for measuring glucose, glycated protein, and total protein capable of measuring at least one among levels of the glucose, glycated protein, and total protein by using the biosensor array.

Description

[0001] The present invention relates to a bio-sensor array for measuring glucose, glycated protein and total albumin,

The present invention relates to a biosensor array capable of simultaneously measuring blood sugar, glycated protein and total protein in blood.

Materials with small nanoscale sizes have recently become very important because of their unique electrical, optical and mechanical properties. Studies on nanostructures that have been carried out so far have shown new potentials such as quantum size effect and their applicability as a future optical device. In particular, nanostructures can be used not only as single electron transistor devices but also as various chemical sensors and biosensors, and more attention is focused on them.

The detection of a target substance using a biosensor including a nanostructure is performed by adsorbing a target substance (chemical agent, biomolecule, disease marker) to be detected by a receptor immobilized on the surface of the nanostructure on the surface of the nanostructure, The target material is implemented in such a way as to cause a change in conductivity of the nanostructure.

Such changes in the conductivity of nanostructures may be used to detect target substances in the body, for example, blood glucose and blood proteins in diabetic patients.

Diabetes is a type of metabolic disorder in which insulin secretion from the pancreas is deficient or not functioning normally. Diabetics receive diet, exercise, and medication to manage the sugar in the blood. Therefore, it is essential for diabetics to regularly measure glucose levels daily to receive the therapy.

Conventionally, as a method for diagnosing the sugar level, there is a method of introducing a small amount of blood sample collected by sticking a finger of a patient into a chemically processed sensor and measuring the concentration of the blood component. Such a method is disclosed in Korean Patent Laid-Open No. 10-2010-0086039.

The conventional glucose measuring apparatus measures only the instantaneous glucose measurement of blood sugar and it is difficult to know the improvement of the disease of diabetic patients. Knowing the average change in blood glucose level allows you to know the amount and degree of protein in the body that the sugar has glycosylated. This change in body protein tells about improvement or aggravation of diabetes mellitus patients and also serves as an indicator of the possibility of complications due to diabetes.

Currently, personal devices capable of self-monitoring blood glucose measurement have been developed, but there is no device capable of measuring the proportion of glycated proteins due to blood sugar.

KR 10-2010-0086039 (application number)

The present invention provides a biosensor array capable of simultaneously measuring the amount of blood glucose, glycated protein, and total protein even with a small amount of blood.

The present invention relates to a substrate; A first sensing electrode formed on the substrate, a first source electrode connected to both ends of the first sensing electrode and a first drain electrode, and a glucose sensor attached to the first sensing electrode, Oxydase); A second sensing electrode formed on the substrate, a second source electrode and a second drain electrode connected to both ends of the second sensing membrane, and a saccharide protein receptor attached to the second sensing membrane, A second sensor including an albumin antibody, the second sensor being adjacent to the first sensor; And a third sensing electrode formed on the substrate, a third source electrode and a third drain electrode connected to both ends of the third sensing film, and a total protein receptor attached on the third sensing film, And a third sensor adjacent to the second sensor, wherein the first sensor, the second sensor, and the third sensor are coated with the treated blood, and the blood glucose receptor, the glycoprotein receptor, and the total protein The receptor binds to the blood glucose, glycated protein and total protein present in the blood, measures currents flowing through the first sensing membrane, the second sensing membrane and the third sensing membrane to measure blood glucose, glycated protein and total protein simultaneously The biosensor array being characterized in that the biosensor array is measured.

Also, the present invention provides a method for measuring blood glucose, glycated protein and total protein, wherein at least one of the values of blood glucose, glycated protein and total protein is measured using the biosensor array.

The present invention can simultaneously measure blood glucose levels, diabetes mellitus, or weakness by measuring blood sugar, glycated protein and total protein in a diabetic patient simultaneously, and it is possible to easily measure blood glycated proteins in real time.

1 is a view showing a configuration of a biosensor array according to an embodiment of the present invention.
2 is a view showing a basic configuration of a first sensor according to an embodiment of the present invention.
3 is a diagram showing a basic configuration of a second sensor according to an embodiment of the present invention.
4 is a diagram showing a basic configuration of a third sensor according to an embodiment of the present invention.
FIG. 5 is a graph showing blood glucose, glycated protein and total protein measured in real time using a biosensor array according to an embodiment of the present invention.

The present invention relates to a biosensor array, and more particularly, A first sensing electrode formed on the substrate, a first source electrode connected to both ends of the first sensing electrode and a first drain electrode, and a glucose sensor attached to the first sensing electrode, Oxydase); A second sensing electrode formed on the substrate, a second source electrode and a second drain electrode connected to both ends of the second sensing membrane, and a saccharide protein receptor attached to the second sensing membrane, A second sensor including an albumin antibody, the second sensor being adjacent to the first sensor; And a third sensing electrode formed on the substrate, a third source electrode and a third drain electrode connected to both ends of the third sensing film, and a total protein receptor attached on the third sensing film, And a third sensor adjacent to the second sensor, wherein the first sensor, the second sensor, and the third sensor are coated with the treated blood, and the blood glucose receptor, the glycoprotein receptor, and the total protein The receptor binds to the blood glucose, glycated protein and total protein present in the blood, measures currents flowing through the first sensing membrane, the second sensing membrane and the third sensing membrane to measure blood glucose, glycated protein and total protein simultaneously .

On the other hand, an oxide layer is additionally provided between the substrate and the first sensing film, between the substrate and the second sensing film, and between the substrate and the third sensing film, and the upper surface of the oxide layer may be a hydrophobic surface , The oxide layer may be formed by a thin film forming method.

In addition, the materials of the first to third sensing films may be carbon nanotubes, and the materials of the first to third source electrodes and the first to third drain electrodes may include gold

In particular, the first to third drain electrodes may be a common electrode.

The blood glucose receptor of the present invention is attached to the first sensing membrane by a first functional group, and the first functional group may be any one selected from the group consisting of an amine group, a carboxyl group and a thiol group.

The glycated protein receptor of the present invention may be attached to the second sensing membrane by a second functional group, and the second functional group may be any one selected from the group consisting of an amine group, a carboxyl group, and a thiol group.

Also, the total protein receptor of the present invention may be attached to the third sensing membrane by a third functional group, and the third functional group may be any one selected from the group consisting of an amine group, a carboxyl group and a thiol group.

The glycoprotein may be at least one of glycated albumin (gHSA), glycated IgG, and glycated IgM, and the total protein may be one of total albumin (HSA), IgG and IgM have.

In addition, the biosensor array of the present invention may further include a protective film capable of covering the first sensor, the second sensor, and the third sensor. In addition, the material of the protective film includes plastic, and fine holes may be formed.

The treated blood may be a mixture of serum and plasma and a buffer solution.

Also, the present invention relates to a method for measuring blood glucose, glycated protein and total protein, characterized in that at least one of the values of blood glucose, glycated protein and total protein is measured using a biosensor array.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The sizes and shapes of the components shown in the drawings may be exaggerated for clarity and convenience. In addition, terms defined in consideration of the configuration and operation of the present invention may be changed according to the intention or custom of the user, the operator. Definitions of these terms should be based on the content of this specification.

It is to be understood that within the scope of the appended claims, the invention may be practiced otherwise than as specifically described within the spirit and scope of the appended claims. Of course.

FIG. 1 is a view showing a configuration of a biosensor array according to an embodiment of the present invention. FIG. 2 is a diagram showing a basic configuration of a first sensor according to an embodiment of the present invention. FIG. 4 is a diagram illustrating a basic configuration of a third sensor according to an embodiment of the present invention. FIG. 5 is a diagram showing a basic configuration of a second sensor according to an embodiment of the present invention. A graph showing the results of measuring blood glucose, glycated protein and total protein in real time. Hereinafter, the biosensor array of the present invention will be described in detail with reference to FIGS. 1 to 5 and embodiments.

The present invention relates to a biosensor array capable of simultaneously measuring the values of blood glucose, glycated protein and total protein in blood as described above.

As shown in FIG. 1, the biosensor array of the present invention includes a substrate 100, a first sensor 10, a second sensor 11, and a third sensor 12.

1 to 4, the first sensor 10 of the present invention includes a first sensing layer 300 formed on the substrate 100, a first source connected to both ends of the first sensing layer 300, And a blood glucose receptor 600 attached to the electrode 400 and the first drain electrode 501 and the first sensing film 300 to couple with the blood glucose. And an oxide layer 200 may be further formed between the first electrode layer 300 and the second electrode layer 300.

The second sensor 11 of the present invention includes a second sensing layer 310 formed on the substrate 100, a second source electrode 410 connected to both ends of the second sensing layer 310, A drain electrode 502 and a saccharide protein receptor 610 attached to the second sensing layer 310 and coupled to the saccharide protein may be included between the substrate 100 and the second sensing layer 310, And may further include an oxide layer 200.

The third sensor 12 of the present invention includes a third sensing layer 320 formed on the substrate 100, a third source electrode 420 connected to both ends of the third sensing layer 320, Drain electrode 503 and a third sensor 12 attached to the third sensing film 320 to form a total protein receptor 620 that binds to the total protein. 3 sensing layer 320 may further include an oxide layer 200.

The substrate 100 of the present invention serves as a substrate for measuring blood glucose, glycated protein, and total protein, and may be a part of a general silicon wafer, but is not limited thereto. In some cases, a substrate of glass, Can be used.

1 to 4, the substrate 100 is shown to have a size limited to the first sensor 10, the second sensor 11, and the third sensor 12. However, as shown in FIG. 1, A plurality of sensors, that is, a first sensor 10, a second sensor 11, and a third sensor 12 may be formed on the substrate 100. Although FIG. 1 shows the arrangement of three sensors, the present invention is not limited thereto, and the number of sensors can be increased or decreased in accordance with the object of the present invention.

In addition, the oxide layer 200 of the present invention can serve as a gate insulating film. The oxide layer 200 can be formed using physical vapor deposition (PVD), chemical vapor deposition (CVD) or the like, but not limited thereto, and a known thin film formation method can be used without limitation.

The first, second, and third sensing films 300, 310, and 320 may be formed on the oxide layer 200. When a certain amount of blood sugar is adsorbed on the surface of the oxide layer 200, So that the current flows between the drain electrodes. Here, the pair of source and drain electrodes are a first source electrode 400 and a first drain electrode 501, a second source electrode 410 and a second drain electrode 502, The third drain electrode 420 and the third drain electrode 503, respectively. In particular, in order to easily grasp whether the target materials 700, 710, and 720 are contained in the solution in excess of the threshold value, the sensing film of the present invention may be composed of a material having a high band gap energy. Particularly, carbon nanotubes (CNTs) can be used as a substance constituting the sensing film of the present invention. The carbon nanotubes used as the sensing film may have a single wall, a double wall, a multiwall, or the like, and may have a rope shape in some cases. Here, the target materials 700, 710, and 720 may refer to blood glucose, glycated proteins, or total proteins, and the sensing film may include a first sensing film 300, a second sensing film 310, May be the sensing film 320.

Generally, carbon nanotubes may be substances that exhibit strong hydrophobicity. Therefore, the upper surface of the oxide layer 200 may be further modified to be hydrophobic in order to easily form a sensing film composed of carbon nanotubes on the oxide layer 200. However, in the present invention, the upper surface of the oxide layer 200 is not limited to the hydrophobic modification, and if necessary, the upper surface of the oxide layer 200 is modified to have a hydrophilic property with strong electrical polarity Lt; / RTI >

In addition, the source electrode and the drain electrode of the present invention may be disposed opposite to each other at regular intervals at both ends of the sensing film. The pair of the source electrode and the drain electrode can play a role of causing a current to flow when a certain amount or more of the target material is adsorbed on the surface of the sensing film. As the source electrode and the drain electrode, gold (Au) may be used. On the other hand, the source electrode can function as an individual electrode and the drain electrode can function as a common electrode 500 as described later. Here, the source electrode means the first source electrode 400, the second source electrode 410 and the third source electrode 420, and the drain electrode means the first drain electrode 501, The second drain electrode 502 and the third drain electrode 503, and the target material may refer to a blood sugar, a glycated protein, or a total protein.

In addition, the receptor of the present invention may be attached on the sensing membrane to perform binding with the target material 700, 710, 720.

Meanwhile, the target materials 700, 710, and 720 may be attached to the sensing film in combination with the receptor, thereby allowing the current to flow to the unit sensor. Therefore, when a large amount of target material is contained in a certain detection solution, a large amount of current flows into the unit sensor, and when a target substance is contained in a certain detection solution, a small amount of current flows in the unit sensor . Of course, the current may not flow in some cases.

The receptors may be blood glucose receptors 600, glycated protein receptors 610, and total protein receptors 620 of the present invention. In particular, it may be an albumin antibody that is a glucose receptor (GOD) (Glucose Oxydase), a glycated albumin antibody that is a glycated protein receptor (610), and a total protein receptor (620).

In addition, the blood glucose receptor 600 includes GOD (Glucose Oxydase). More specifically, the blood glucose receptor 600 is attached to the first sensing membrane 300 by a first functional group, May be any one selected from the group consisting of an amine group, a carboxyl group and a thiol group.

In addition, the glycated protein receptor 610 may be any one or more selected from the group consisting of an enzyme substrate, a ligand, an amino acid, a peptide, a protein, a nucleic acid, a lipid and a carbohydrate, and more specifically, a glycated albumin antibody. The albumin antibody binds to a specific antibody protein in the blood, that is, glycated albumin (gHSA), and causes an antigen-antibody reaction. The glycated albumin antibody does not bind to other components in the blood, interference is minimized, and only the desired glycated albumin (gHSA) value can be measured. In addition, the glycated protein receptor 610 may be attached to the second sensing membrane 310 by a second functional group, and the second functional group may be any one selected from the group consisting of an amine group, a carboxyl group, and a thiol group .

In addition, the total protein receptor 620 may be any one or more of the group consisting of a ligand, an amino acid, a peptide, a protein, a nucleic acid, a lipid, and a carbohydrate, for example, an albumin antibody. The albumin antibody is a substance capable of detecting both albumin and glycosylated albumin. Also, the total protein receptor 620 may be attached to the third sensing layer 320 by a third functional group, and the third functional group may be any one selected from the group consisting of an amine group, a carboxyl group, and a thiol group.

In addition, the first functional group, the second functional group and the third functional group of the present invention may be any one or more selected from the group consisting of an amine group, a carboxyl group and a thiol group as described above, and more specifically, Molecules. Such as phenylboronic acid, naphthaleneboronic acid, phenanthreneboronic acid, pyreneboronic acid, and polymeric boronic acid.

The biosensor array of the present invention may further include a protective layer (not shown) covering the first sensor 10, the second sensor 11, and the third sensor 12. More specifically, the biosensor array of the present invention can be protected from external detection solution and impact by covering the substrate 100, the first sensor 10, the second sensor 11, and the third sensor 12 . The protective film may include a plastic or a biological film, and in particular, a minute hole may be formed. In particular, by providing the fine holes, only the blood glucose, the glycated protein and the glycated protein present in the detection solution can be bound to the respective receptors through the fine holes, and the remaining substances can block the internal entry of the biosensor array .

The present invention can also include voltage applying means. More specifically, the voltage applying means may be disposed on the opposite side of the substrate 100 on which the first to third sensing films 300, 310, and 320 are formed. The voltage applying means may apply a positive voltage to the first to third source electrodes 400, 410 and 420 by contacting the first to third source electrodes 400, 410 and 420. The first to third drain electrodes 501, 502 and 503 can be brought into contact with each other to apply a voltage of 1 volt or less to the first to third drain electrodes 501, 502 and 503, It is possible to detect whether a current flows through the wirings connected to the first to third source electrodes 400, 410 and 420 and the first to third drain electrodes 501, 502 and 503 when a voltage is applied .

In addition, the biosensor array of the present invention may include a terminal (not shown) capable of transmitting a current signal to another terminal. A terminal such as a computer or a smart phone may be connected to the terminal, and blood glucose, glycated protein and total protein level can be measured by analyzing the current signal. In particular, the biosensor array of the present invention can be manufactured inexpensively with the same size as a small U.S. range, and can easily measure blood glucose, glycated protein and total protein by disposing through a terminal.

In another embodiment of the present invention, the signals of the currents are analyzed in place of the first to third source electrodes 400, 410, and 420 and the first to third drain electrodes 501, 502, and 503 in the biosensor array, (Not shown) capable of displaying the glycated protein level. The display method may include displaying a glycoprotein level and blood sugar level by providing a display screen in the signal unit, or displaying the glycated protein level and blood sugar using a change in color.

In the above-described biosensor array, the collected blood is applied to the first sensor 10, the second sensor 11, and the third sensor 12 so that the blood glucose receptor 600, the glycoprotein receptor 610 and the total protein receptor 620 are combined with the blood glucose, glycated protein and total protein present in the blood, and the first, second and third sensing films 300, The amount of blood sugar, the glycated protein, and the total protein can be measured at the same time. By simultaneously measuring the blood sugar, the glycated protein and the total protein, the measurement time can be shortened as much as possible.

The first to third sensors 10, 11 and 12 have a structure similar to a metal oxide semiconductor field effect transistor (MOS-FET), and the first to third sensing films 300 The first to third source electrodes 400, 410 and 420 are the source and the first to third drain electrodes 501, 502 and 503 are the drains. Can play a role. Meanwhile, a gate (not shown) may be formed on the opposite surface of the substrate 100 of the present invention. In particular, when carbon nanotubes having properties such as metals and semiconductors are used as the materials constituting the first to third sensing films 300, 310, and 320, the first to third sensors 10, 11, And can function as a carbon nanotube field effect transistor (CNT-FET)

In the present invention, blood glucose refers to glucose contained in the blood, which may mean glucose.

The glycated protein of the present invention may be one of glycated albumin (gHSA), glycated IgG, and glycated IgM.

In addition, the total protein of the present invention may be one of total albumin (HSA), IgG and IgM.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

< Example >

Example  1. Fabrication of biosensor array

First, after a substrate is prepared, an oxide layer such as silicon oxide (SiO ₂ ) is formed thereon. Then, a channel region where a sensing film is to be formed on the oxide layer was patterned by photolithography.

Next, an OTS self-assembled monolayer (SAM) was formed by immersing the substrate in an octadecyltrichlorosilne (OTS) solution having methyl at the terminal end.

Next, a single-walled carbon nanotube solution was prepared by dispersing single-walled nanotubes in 1,2-dichlorobenzene and applying ultrasonic waves. Subsequently, the substrate was immersed in a single-walled carbon nanotube solution for 30 minutes for 30 minutes, sufficiently washed with 1,2-dichlorobenzene, and then dried with nitrogen gas. Through the above process, the single-walled nanotube can be selectively adsorbed on the oxide layer while the OTS SAM having methyl at the terminal end blocks adsorption of the CNT.

Through the above-described method, it is possible to implement a biosensor array in which three CNT-FET circuits, that is, a first sensor, a second sensor and a third sensor, are formed on a substrate of 100 × 100 mm.

Example  2. Blood sugar, Glycation  Measurement of albumin and total albumin values

The values of blood glucose, saccharified albumin and total albumin were determined using the biosensor array of the present invention.

The glucose receptor (GOD) (glucose oxidase, Sigma-Aldrich) was used as the glycoprotein receptor 600. The glycoprotein receptor was a glycated albumin antibody (ARP, American Research Products, NJ, USA [Category: Rabbit Polyclonal Antibody, Immunogen: Serum Albumin purified from Human serum Form: Purified in PBS], and total protein was used as a receptor for albumin antibody (Exocell).

First, a blood sample was dropped on the biosensor array. The blood sample is obtained by mixing serum and plasma of the collected blood with a solution. Here, the solution means buffer solution, and phosphate buffered saline (PBS) was used.

More specifically, 6 μl of a blood specimen sample was applied to the first, second and third sensing membranes, and electrical resistance values of the first to third sensing membranes were measured and derived.

As a result, the current value according to the enzyme reaction of blood glucose was measured on the first sensing membrane for 6 mu l of the blood specimen sample. Specifically, the Ids current measurement was performed using Vg = -2volt and Vds = -1volt, and the sugar concentration was measured from the minimum value of 1.8mg / dl to the 180mg / dl value. In albumin (gHSA), the ratio of the resistance change value according to the antigen-antibody reaction was 1.3283 and the corresponding value of glycated albumin (gHSA) was 78.28 (mg / dl). At this time, the resistance change value in the third sensing membrane according to the antigen-antibody reaction of total albumin (HAS) was 1.34095, and the total albumin (HSA) value (the value of abscissa) was 515 (mg / dl). (Fig. 5)

In addition, the glycation level (%) could be calculated from the values of glycated albumin (gHSA) and total albumin (HSA). The glycation level (%) was calculated as 78.28 (mg / dl) / 515 (mg / dl) * 100 = 15.2 (%).

The embodiments described above are intended to illustrate the contents of the present invention and are not intended to limit the scope of the present invention. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

10: first sensor 11: second sensor
12: Third sensor
100: substrate 200: oxide layer
300: first sensing film 310: second sensing film
320: Third sensing film
400: first source electrode 410: second source electrode
420: third source electrode
500: common electrode 501: first drain electrode
502: second drain electrode 503: third drain electrode
600: blood glucose receptor 610: glycoprotein receptor
620: total protein receptor
700: target substance (blood sugar) 710: target substance (glycated protein)
720: Target substance (total protein)

Claims (15)

Board;
A first sensing electrode formed on the substrate, a first source electrode connected to both ends of the first sensing electrode and a first drain electrode, and a glucose sensor attached to the first sensing electrode, Oxydase);
A second sensing electrode formed on the substrate, a second source electrode and a second drain electrode connected to both ends of the second sensing membrane, and a saccharide protein receptor attached to the second sensing membrane, A second sensor including an albumin antibody, the second sensor being adjacent to the first sensor; And
A third sensing electrode formed on the substrate, a third source electrode and a third drain electrode connected to both ends of the third sensing film, and an albumin, which is a total protein receptor attached to the total protein, An antibody, and a third sensor adjacent to the second sensor,
Wherein the blood glucose receptor, the glycated protein receptor, and the total protein receptor are combined with the blood glucose, glycated protein and total protein present in the blood, and the blood glucose receptor, Wherein a current flowing through the first sensing membrane, the second sensing membrane, and the third sensing membrane is measured to simultaneously measure the values of blood glucose, glycated protein, and total protein. The method according to claim 1,
Further comprising an oxide layer between the substrate and the first sensing layer, between the substrate and the second sensing layer, and between the substrate and the third sensing layer, the upper surface of the oxide layer being a hydrophobic surface Biosensor array. 3. The method of claim 2,
Wherein the oxide layer is formed by a thin film forming method. The method according to claim 1,
Wherein the material of the first to third sensing films is carbon nanotubes. The method according to claim 1,
Wherein a material of the first to third source electrodes and first to third drain electrodes includes gold. The method according to claim 1,
And the first to third drain electrodes are common electrodes. The method according to claim 1,
Wherein the blood glucose receptor is attached to the first sensing membrane by a first functional group, and the first functional group is any one or more selected from the group consisting of an amine group, a carboxyl group, and a thiol group. The method according to claim 1,
Wherein the glycated protein receptor is attached to the second sensing membrane by a second functional group, and the second functional group is any one or more selected from the group consisting of an amine group, a carboxyl group, and a thiol group. The method according to claim 1,
Wherein the total protein receptor is attached to the third sensing membrane by a third functional group, and the third functional group is any one or more selected from the group consisting of an amine group, a carboxyl group, and a thiol group. The method according to claim 1,
Wherein the glycated protein is at least one of glycated albumin (gHSA), glycated IgG, and glycated IgM. The method according to claim 1,
Wherein the total protein is at least one of total albumin (HSA), IgG, and IgM. The method according to claim 1,
Wherein the biosensor array further comprises a protective film covering the first sensor, the second sensor and the third sensor. 13. The method of claim 12,
Wherein the material of the protective film includes plastic, and fine holes are formed therein. The method according to claim 1,
Wherein the treated blood is a mixture of serum and plasma and a buffer solution. A method for measuring blood glucose, glycated protein and total protein, characterized in that at least one of the values of blood glucose, glycated protein and total protein is measured using the biosensor array according to any one of claims 1 to 14.

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