KR20140024549A - Method for manufacturing functional mushroom rice - Google Patents

Abstract

The present invention relates to a technique relating to a method for producing functional native mushroom rice. The functional mushroom native rice, which is produced by inoculating 5-10%(w/w) of a mycelium of Ganoderma lucidum to Korean native white rice or brown rice and culturing the byproduct at 27-29°C for 8-10 days, has excellent anti-complementary activity and anti-diabetic effects. [Reference numerals] (AA) Dry weight of mycelium (g/l)

Description

Functional indigenous mushroom rice and its manufacturing method {Method for manufacturing Functional mushroom rice}

The present invention relates to a functional indigenous mushroom rice and a method for manufacturing the same, and specifically, a technique related to a functional indigenous mushroom rice prepared by inoculating and incubating a mycelium of Ganoderma lucidum on a native rice.

During the Three Kingdoms period from the 6th to 7th century BC, when agricultural farming began, agricultural products became the main food source. In particular, grains, including rice, have served as our staple food so far. Currently, per capita annual rice consumption in Korea peaked at 135.6 kg in 1979 and has been rapidly decreasing to 83.2 kg in 2003 and 76.9 kg in 2007. This is because eating patterns have shifted from rice to fast food or instant food. As a result, the incidence of adult diseases threatening public health is increasing.

On the other hand, as the nutritional excellence of rice is concentrated, research on functional excellence as well as palatability of rice is being actively conducted. Grapo et al reported that blood sugar and insulin secretion were lower when ingesting rice than potatoes and white bread, and that the rice form suppressed the rapid increase in blood sugar levels and insulin secretion compared to processed forms such as flour, porridge, and rice cake, and Kim et al. In addition, mutagenic activity was observed, and processed forms of rice, white snow, and rice flour did not lose their inhibitory activity, and antimutagenic activity was reported. Indigenous rice also has great benefits as a solution to environmentally friendly cultivation methods such as pests due to its nutritional excellence. Recently, research on native rice has been actively conducted to preserve our genetic resources, revitalize local resources and realize eco-friendly agriculture.

Mushrooms belonging to basidiomycetes have been actively researched for bioactive substances and functional ingredients, and have been scientifically proven to have anti-cancer activity, immune activity enhancement, hypoglycemia, lowering lipids, lowering blood cholesterol, and some functional substances have been extracted and purified. It has also been commercialized as a drug. Ingredients exhibiting such efficacy are non-digestible, water-soluble polysaccharides such as beta glucan, beta glucan, heteroglycan, and glycoprotein, and occupy about 10% or more of mushrooms in dry weight.

As a method of using mushrooms, fruiting bodies or liquid culture of mycelium are used to isolate and purify useful components.In the case of using fruiting bodies, some mushrooms cannot be artificially grown in fruiting bodies or are expensive, and thus they are popular for industrialization. There are limitations and disadvantages in that the liquid culture of the mycelium is expensive to separate and purify the useful components. On the other hand, solid culture technology that inoculates mushrooms and incubates them into grains is used as a method of utilizing mushrooms, which does not require a separate extraction and purification process to obtain useful ingredients. Because of this, it contributes to the health promotion of consumers, and has the advantage of increasing the added value of domestic grains.

Republic of Korea Patent Publication No. 10-2006-0036863 (name of the invention: manufacturing method of mushroom rice) is extracted from medicinal, edible mushroom fruiting body with 30 to 95% ethanol and coated on grain rice to adjust the water content 30 to 40% It is disclosed for a method of producing a mushroom rice, characterized in that the inoculated mushroom sterilization for 15 to 30 minutes at 121 ℃ and then incubated at a constant temperature for 1 to 2 months by inoculating the mushroom spawn corresponding to cooling to room temperature again have.

It is an object of the present invention to provide a functional indigenous mushroom rice prepared by culturing Ganoderma lucidum mycelium in native white rice or brown rice.

In addition, an object of the present invention is to provide a functional native mushroom rice excellent in the immune boosting and hypoglycemic effect for diabetic patients.

In order to achieve the above object, the present invention provides a method for preparing native white rice mushroom rice, which is prepared by inoculating 7.5-12.5% (w / w) Ganoderma lucidum mycelium on native white rice and incubating at 27-29 ° C. for 8 to 10 days. .

In another aspect, the present invention provides a method for producing a native brown rice mushroom rice prepared by inoculating 7.5 ~ 12.5% (w / w) in Gaji mushroom mycelium in native brown rice and incubated for 8 to 10 days at 27 ~ 29 ℃.

In the present invention, ganoderma lucidum mycelium is used, according to the experiment of the present invention Ganoderma Ganodema lucidum ), Pleurotus ostreatus ), Pleurotus eryngii ), Hericium beetle erinaceus ), Phellinus linteus , Cordyceps militaris ), and shiitake ( Lintus edodes and sore throat ( Auricularia) Auricula -jude ) cultured mycelia and anti-complement activity showed that Ganoderma lucidum showed the highest anti-complement activity.

In addition, the present invention inoculate the Ganoderma lucidum mycelium 7.5 ~ 12.5% (w / w), if the content of the mycelium culture medium is less than 7.5% can be difficult to obtain the expected effect, when exceeding 12.5% significant effect increase by the amount added It may not appear economically.

In the present invention, as a method for culturing Ganoderma lucidum mycelium, a culture method commonly used in the art to which the present invention pertains may be used. Preferably, MCM (MCM, mushroom complete medium; glucose 2%, MgSO ₄ 7H ₂ O 0.05%, KH ₂ PO ₄ 0.046%, K ₂ HPO ₄ 0.1%, yeast extract 0.2%, peptone 0.2%, pH 4.5) It is recommended to culture the liquid at a temperature of 30 ~ 33 ℃ in the medium.

On the other hand, Ganodema mushroom lucidum ) occurs in the broad-leaved roots in summer and is also known as the Bulchocho of Qin Shi Huang. Tonic, Jinhae, swelling (효능) and the effect is effective in respiratory diseases, nervous breakdown, heart disease, high blood pressure, lower cholesterol and anti-cancer effects are known.

In addition, the functional native mushroom rice of the present invention can be prepared in various forms of food using rice. For example, it may be prepared from rice drinks, rice cakes, rice cakes, porridge, and the like.

Functional native mushroom rice of the present invention prepared as described above has an advantage that can be provided to diabetic patients with excellent anti-complement activity and anti-diabetic effect. In addition, the mushroom mycelium culture can be easily consumed as a whole rice, so it can contribute to the health of consumers.

The present invention can provide a functional native mushroom rice with physiological activity by culturing the Ganoderma lucidum mycelium in native white rice or brown rice.

In addition, the functional native mushroom rice of the present invention has an immune enhancing and hypoglycemic effect, thereby promoting the development and consumption of food for diabetic patients.

1 is a graph showing the mycelial production of eight mushrooms [GL: Ganoderma lucidum ( Ganodema) lucidum ), PO: Pleurotus ostreatus ), PE: Pleurotus eryngii ), HE: Hericium erinaceus ), PL: Phellinus linteus ), CM: Cordyceps militaris ), LE: Shiitake ( Lintus edodes ), AA: Auricularia auricula - jude )].
Figure 2 is a schematic diagram illustrating a process for recovering the intracellular polysaccharides from the liquid culture mycelium culture of eight mushrooms.
3 is a graph showing the anti-complement activity of eight mushroom mycelium [GL: Ganoderma lucidum ( Ganodema) lucidum ), PO: Pleurotus ostreatus ), PE: Pleurotus eryngii ), HE: Hericium erinaceus ), PL: Phellinus linteus ), CM: Cordyceps militaris , LE: Shiitake ( Lintus) edodes ), AA: Auricularia auricula - jude ), LPS: Lipopolysaccharide].
Figure 4 is a graph showing the mycelium production of Ganoderma lucidum according to pH.
5 is a graph showing the mycelium production of Ganoderma lucidum according to temperature.
6 is a view schematically showing a process of culturing mushroom mycelium using native rice.
Figure 7 is a photograph of the mushroom rice (A) and cultured white rice Ganoderma lucidum rice (B), brown rice Ganoderma lucidum rice (C) in culture.
8 is a graph showing the anti-complement activity of Ganoderma lucidum rice (GL: Ganoderma lucidum mushroom, NR: native rice, NRCGL: native Ganoderma lucidum rice, LPS: Lipopolysaccharide).
9 is a graph showing the weight change of the experimental rat according to the intake of native Ganoderma lucidum rice.
10 is a graph showing the change in dietary intake of experimental rats according to the intake of native Ganoderma lucidum rice.
Figure 11 is a graph showing the blood glucose changes in experimental rats according to the intake of native Ganoderma lucidum rice.

Hereinafter, the content of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.

[ Example  1: Mushroom Mycelial Culture]

In this example, the mycelial production of eight mushrooms was compared.

Ganodema lucidum ), Pleurotus ostreatus ), Pleurotus eryngii , Herringium ( Hericium) erinaceus ), Phellinus linteus ), Cordyceps militaris ), shiitake ( Lintus edodes and sore throat ( Auricularia) auricula -jude ) prepared mushrooms, these were MCM (mushroom complete medium; glucose 2%, MgSO ₄ 7H ₂ O 0.05%, KH ₂ PO ₄ 0.046%, K ₂ HPO ₄ 0.1%, yeast extract 0.2%, peptone 0.2%, pH 4.5] was cultured in liquid.

As a result of the culture, mycelium production of Ganoderma lucidum and Pleurotus eryngii was the highest under the same conditions (see FIG. 1).

[ Experimental Example  1: Mushroom Mycelium Anti-complement  Active comparison]

Prepared in Example 1. In this experiment, for example, Ling Zhi (Ganodema lucidum ), Pleurotus ostreatus ), Pleurotus eryngii ), Hericium erinaceus , Phellinus linteus ), Cordyceps militaris ), shiitake ( Lintus edodes and sore throat ( Auricularia) Auricula - jude ) was intended to measure the anti-complement activity by recovering the intracellular polysaccharide from the mycelium liquid culture of the mushroom (see Fig. 2).

Anti-complement activity was measured by the Mayer method. 50 μL of NHS (NHS, Normal Human Serum), GVB ++ (GVB ++, Gelatin Veronal Buffered saline, pH 7.2) and the sample are mixed and reacted at 37 ° C for 30 minutes, and then GVB ++ is added to the reaction solution. 350 μl each was added, and serial dilutions were carried out from 10 to 160 times. 750 μl of GIBB (GVB ++) and positive sensitized blood cells (108 cells / mL) were added and then 250 μl each was reacted for 1 hour. 2.5 ml of PBS (PBS, Phosphate Buffered Saline, pH 7.4) was added thereto, followed by centrifugation, and the absorbance of the supernatant was measured at 412 nm.

Anti-complement activity is indicated by ITCH50, Inhibition of 50% Total Complement Hemolysis. In order to verify the correct activity of the sample, LPS (LPS) with known titer is used as a reference material. The calculation method is shown in Equation 1 below.

As a result of the measurement (see FIG. 3), the LPS (LPS), a standard, but lower than the Ganoderma lucidum and situation mushrooms showed the highest activity over the 70 (ITCH ₅₀ ) value over 70.

As a result of considering mycelial production and anti-complement activity, Ganoderma lucidum showed the highest mycelial production and anti-complement activity.

[ Example  2: Comparison of Liquid Culture Conditions for Ganoderma lucidum Mycelium]

In this example, it was intended to establish the liquid culture conditions of Ganoderma lucidum for high mycelial production.

The results showed the highest mycelial yields at pH 4.5 and 5.0 (see FIG. 4) and temperature at 30 ° C. (see FIG. 5). As a result, it was confirmed that the optimum culture conditions of Ganoderma lucidum mycelium liquid culture was pH 4.5 and the temperature 30 ℃.

[ Example  3: Mushroom Mycelial Culture Using Native Rice According to Culture Conditions]

In this example, to compare the culture form of mushroom rice according to the culture conditions (see Figure 6).

Example  3-1: Comparison of Culture Type and Duration of Mushroom Rice According to Native Rice Input

 To sterilize the rice, sterilize it with 1 ~ 2.5 kg of rice in a sterilizable bag size of 32 * 44.5 cm, and soak it in water overnight, drain the water and put the grain soaked in the plastic bag. Autoclaved). After inoculating liquid cultured Ganoderma lucidum mycelium on autoclaved indigenous rice and incubated for 2 to 3 weeks in an incubator fixed at 25 ℃.

input Physical form Incubation period (days) 1.0 kg +++++ 10 1.5 kg ++ 14 2.0 kg + 20 2.5 kg + 25

As a result (see Table 1), 1 kg of native rice showed the best physical form and incubation period.

Example  3-2: Comparison of Culture Type and Duration of Mushroom Rice According to Inoculation Volume

To sterilize the rice, put the same amount of rice in a sterilizable bag size of 32 * 44.5 cm and sterilize it. Sterilized. After inoculating up to 5 ~ 17.5% in liquid cultured Ganoderma lucidum mycelium in high pressure sterilized indigenous rice was incubated for 2 to 3 weeks in a fixed incubator at 25 ℃.

Inoculation amount (%) Physical form Incubation period (days) 5.0 ++ 13 7.5 ++ 12 10.0 ++++ 10 12.5 +++ 9 15.0 ++ 9 17.5 + 9

As a result (see Table 2), 10% inoculation of Ganoderma lucidum mycelium culture medium in native rice showed the optimum physical form and incubation period.

Example  3-3: Comparison of Culture Type and Duration of Mushroom Rice According to Temperature

To sterilize the rice, put the same amount of rice in a sterilizable bag size of 32 * 44.5 cm and sterilize it. Sterilized. After inoculating liquid cultured Ganoderma lucidum mycelium on autoclaved indigenous rice, the temperature was incubated for 2 to 3 weeks in an incubator at 22-90 ° C.

Temperature (℃) Physical form Incubation period (days) 22 ++ 15 25 +++ 11 28 ++++ 10 31 +++ 11

As a result (see Table 3), the physical form and the incubation period were optimal when the culture temperature was 28 ℃.

Example  3-4. Comparison of Mushroom Rice Culture Types According to Culture Period

To sterilize the rice, put the same amount of rice in a sterilizable bag size of 32 * 44.5 cm and sterilize it. Sterilized. After inoculating liquid cultured Ganoderma lucidum mycelium on autoclaved indigenous rice, the temperature was incubated for 8 to 13 days in an incubator fixed at 25 ° C.

Incubation period (days) Color Mushroom incense Physical form 8 ++ ++ +++ 9 ++++ +++ +++ 10 ++++ +++ +++ 11 +++ +++ +++ 12 ++ ++++ +++ 13 + +++ +++

In the results (see Table 4), color was the best when the culture period was 9 days and 10 days, mushroom flavor was the strongest at 12 days, the physical shape was good between 8 and 13 days. As a result of comparison, it was determined that 9 days was the most suitable for the culture period.

Therefore, in the present invention, inoculating 10% of Ganoderma lucidum spawn seed on native rice and culturing for 9 days at 28 ° C confirmed that the optimal culture condition of native mushroom rice.

[ Experimental Example  2: of native Ganoderma lucidum rice Anti-complement  Activity measurement]

In this experimental example, the anti-complement activity of native Ganoderma lucidum rice prepared under the optimum culture conditions established in Example 3 was measured.

The measuring method was performed by the same method as described in Experimental Example 1.

As a result of the measurement (see FIG. 8), the native Ganoderma lucidum rice had a lower anticomplement activity than the Ganoderma lucidum itself, but was higher than that of native ganoderma lucidum, and thus the anticomplement activity of Ganoderma lucidum rice was confirmed.

[ Experimental Example  3: native Ganoderma lucidum rice Anti-diabetic  Activity measurement]

Experimental Example  3-1: Preparing the Experiment

First, Sprague-Dawley male rats weighing 100-120 g were purchased from the experimental animal center and maintained at 22 ± 0.5 ° C, 55 ± 5% humidity, and a 12-hour light cycle. One by one in a stainless steel cage (Stainless steel cage) was adapted to the laboratory environment for a week and then used in the experiment.

In addition, diabetes induction affects only the beta-cell of the pancreas and does not affect other organs, and streptozotocin (STZ), which is known to cause hyperglycemia due to lack of insulin Was dissolved in 0.1 M citrate and injected intramuscularly (50 mg / kg body weight). The blood glucose level in the tail vein was measured 24 hours after streptozotocin (STZ) injection. 300 mg / dl or more was considered diabetic rat and used for experiment. Hyperglycemic-induced rats of 8 rats were placed in each group to have similar blood glucose values and mean weight, and water and feed were freely consumed during the experiment.

Diabetes-induced experimental groups were divided into the group fed with native white rice and brown rice, the group fed with native white rice and brown rice mushroom rice, and the control group fed with saline, and then orally administered the feed 100 mg / kg body weight for 2 weeks. It was. Blood glucose levels were measured by collecting blood from the tail of experimental mice every 2 days after diabetes induction. The body weight of the test animals was measured every day at a certain time in the morning, and the feed intake was measured by collecting the remaining amount immediately before the weight measurement for each test group.

In addition, the experimental results were analyzed and compared using the statistical package of social science (SPSS) program, and expressed as mean ± standard error (SE) for each experimental group through analysis of variance (ANOVA). It was. The statistical significance of the mean value between each experimental group was tested by Duncan's multiple range test at p <0.05.

Experimental Example  3-2: Comparison of Body Weight and Dietary Intake of Rats with Indigenous Mushroom Rice

Native white rice Ganoderma lucidum rice and native brown rice Ganoderma lucidum rice were fed to diabetic rats for 14 days, and then the weight and dietary intake of each group were compared.

As a result of comparing the weight of each group (see FIG. 9), the diabetic-induced groups gained weight over the breeding time than the control group, but were less than the normal group. This is because streptozotocin selectively destroys the beta cells of the pancreas (β-cell), leading to abnormalities in insulin (insulin) production, resulting in an imbalance of sugar metabolism, and thus, weight is not easily recovered. However, in the diabetic induction group, indigenous white rice and brown rice ganoderma lucidum rice groups gained more weight than the control group, and insulin secretion was observed in streptozotocin-induced diabetic rats by ingesting ganoderma lucidum rice. Is thought to increase slightly.

In addition, as a result of comparing the dietary intake of each group (see FIG. 10), the dietary intake of the diabetes-induced group was lower than the dietary intake of the control group. It was judged that this was due to a phenomenon called polyphagia among the symptoms of diabetes. As can be seen from the above results, the native mushroom rice of the present invention showed excellent antidiabetic activity.

Experimental Example  3-3: Organ Weight Measurement in Experimental Rats

In order to measure the weight of the liver, kidney, pancreas and spleen of the experimental rats in which the breeding was completed, each organ was removed from the experimental animals, and the fat and connective tissues were removed, and then washed with physiological saline (0.9% NaCl), and then water. After the removal was weighed.

Experimental group Liver (g) Height (g) Spleen (g) Pancreas (g) Normal group 9.31 ± 0.23 ^NS 2.14 ± 0.03 ^NS 0.49 ± 0.02 ^NS 0.50 ± 0.02 ^NS Control group 9.82 ± 0.25 2.33 ± 0.04 0.52 + - 0.01 0.48 + 0.04 White rice 9.47 ± 0.26 2.26 ± 0.03 0.53 ± 0.02 0.49 + 0.02 Brown rice 9.21 ± 0.25 2.31 ± 0.05 0.55 ± 0.03 0.47 ± 0.05 Baekmi Ganoderma lucidum rice 9.24 ± 0.22 2.19 ± 0.02 0.51 ± 0.03 0.47 ± 0.03 Brown Rice Mushroom Rice 9.41 ± 0.24 2.26 ± 0.03 0.57 + 0.02 0.51 ± 0.04 Each value is the mean ± SE (n = 8)
NS: Not significant

Diabetic rats were fed with white rice and brown rice ganoderma lucidum rice for 14 days, and then weighed rat liver, kidney, spleen and pancreas (see Table 5). There was no difference.

Experimental Example  3-4: Comparison of Blood Glucose Levels in Experimental Rats According to Intake of Native Mushroom Rice

9 hours before sacrifice, the rats were fasted and water was supplied. Anesthetized with ether, and blood collected by a syringe from the abdominal aorta was centrifuged at 4 ° C (1,110 × g / 15 minutes) and used as plasma. Plasma glucose was measured using glucose oxidase method.

Comparing the changes in blood sugar levels of the white rice and brown rice ganoderma lucidum rice in the diabetic rats induced for 14 days (see FIG. 11), the hyperglycemia was observed in all of the diabetic rats, but the indigenous brown rice and white rice were administered. One group and the group treated with native brown rice and white rice ganoderma lucidum rice showed significantly lower blood sugar than the control group. In particular, the indigenous white rice and brown rice ganoderma lucidum rice groups were found to reduce blood sugar by about 30% compared to the control group to increase insulin secretion and alleviate damage to pancreatic beta cells (β-cell).

Experimental Example  3-5: Serum Total Cholesterol and Plasma of Rats Treated with Indigenous Mushroom Rice Triglyceride  Content comparison

Metabolic features of diabetes are elevated blood sugar and abnormal lipid metabolism. Increasing blood sugar can induce lipid peroxidation and induce atherosclerosis, and poorer control of blood sugar leads to severe hyperlipidemia, which reduces the removal rate of very low-density lipoproteins in the blood, thus reducing total cholesterol in the blood. cholesterol and triglycerides are increased. The purpose of this study was to determine the antidiabetic activity of native mushroom rice intake by comparing blood total cholesterol and triglyceride contents.

 9 hours before sacrifice, the rats were fasted and water was supplied. Anesthetized with ether, and blood collected by a syringe from the abdominal aorta was centrifuged at 4 ° C (1,110 × g / 15 minutes) and used as plasma. Total cholesterol (tri-cholesterol) and triglyceride (triglyceride) were measured by the Buccoro (Bucolo) method to determine the total cholesterol and triglyceride content of blood in experimental rats according to the intake of native mushroom rice.

Experimental group Total cholesterol (㎎ / ㎗) Triglycerides (mg / dl) Normal group 70.32 ± 3.42 ^a 60.56 ± 3.01 ^a Control group 98.71 ± 4.28 ^c 90.51 ± 5.42 ^c White rice 87.78 ± 5.27 ^b 82.51 ± 5.23 ^bc Brown rice 89.15 ± 6.23 ^b 83.53 ± 4.72 ^bc White rice Ganoderma lucidum rice 80.12 ± 3.28 ^ab 76.52 ± 6.12 ^b Brown rice ganoderma lucidum rice 79.16 ± 4.81 ^ab 77.12 ± 3.38 ^b Each value is the mean ± SE (n = 8).
^{a, b, c} Values with different superscript letters in the same column significantly different among the groups at p <0.05.

As a result of the measurement (see Table 6), the diabetic inducing group showed higher total cholesterol and triglyceride in the blood than the normal group, but lower than the control group. From the results, it was confirmed that ingestion of native white rice and brown rice ganoderma lucidum rice significantly inhibited the increase of total cholesterol and triglyceride due to diabetes.

Experimental Example  3-6. Indigenous mushroom rice intake AST  And ALT  Activity measurement

ALT and AST activity are enzymes that are present in large amounts in hepatocytes and are used as indicators of hepatic damage as they are leaked out of the cells and increased in the blood when the liver is damaged. In diabetes, hepatic lipid metabolism abnormality causes hepatic damage, which is known to increase ALT and AST. In this experiment, we tried to measure the activity of AST and ALT according to the consumption of native mushroom rice.

9 hours before sacrifice, the rats were fasted and water was supplied. Anesthetized with ether, and blood collected by a syringe from the abdominal aorta was centrifuged at 4 ° C (1,110 × g / 15 minutes) and used as plasma. The AST and ALT activities were measured using a kit (kit, Sigma Co.) by the Reitman-Frankel method.

Experimental group ALT (IU / L) AST (IU / L) Normal group 26.45 ± 0.92 ^a 87.75 ± 4.19 ^a Control group 45.42 ± 4.64 ^b 119.23 ± 5.14 ^b White rice 38.73 ± 0.61 ^ab 102.05 ± 3.46 ^ab Brown rice 38.07 ± 1.31 ^ab 100.31 ± 5.91 ^ab White rice Ganoderma lucidum rice 33.17 ± 2.24 ^ab 97.28 ± 4.75 ^ab Brown rice ganoderma lucidum rice 31.25 ± 2.24 ^ab 93.11 ± 3.29 ^ab Each value is the mean ± SE (n = 8).
^{a, b, c} Values with different superscript letters in the same column significantly different among the groups at p <0.05.

As a result of the measurement (see Table 7), diabetic-induced groups showed high levels of ALT and AST activity, presumably due to high blood sugar damage to the liver. In addition, the indigenous brown rice and white rice ganoderma lucidum rice group showed lower ALT and AST activity than the control group, it can be seen that the intake of native brown rice and white rice ganoderma lucidum rice recovers to some extent hepatic damage caused by hyperglycemia.

Claims (2)

Method of producing a functional native white rice mushroom rice, characterized in that inoculated 7.5 ~ 12.5% (w / w) to Ganoderma lucidum mycelium in native white rice and incubated at 27 ~ 29 ℃ for 8 to 10 days.
Method of producing a functional indigenous brown rice mushroom rice, characterized in that inoculated 7.5 ~ 12.5% (w / w) in the local brown rice mycelium cultured for 8 to 10 days at 27 ~ 29 ℃.

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