KR20120038586A - Cosmetic composition for improving skin senescence - Google Patents

Abstract

PURPOSE: A cosmetic composition for improving anti-aging is provided to ensure antioxidation, anti-wrinkling, skin whitening, and skin moisturizing. CONSTITUTION: A composition for improving anti-aging contains 60-80 wt% of mixture oriental medicinal extract including Chrysanthemum indicum L. and Eriocaulon sieboldianum SIEB., and 20-40 wt% of mixture fruit/seed extract including Arctii Fructus, Xanthii Fructus, Kochia scoparia, Cuscuta Chinensis Lam., Amygdalaceae, Celastraceae, or Sophora japonica L. The composition is manufactured in the form of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

Description

Cosmetic composition for skin aging improvement {COSMETIC COMPOSITION FOR IMPROVING SKIN SENESCENCE}

The present invention is a combination of herbal extracts and mixed fruit / seed extracts effective in antioxidant activity, wrinkle improvement, skin whitening and skin moisturizing complexes to improve the various symptoms according to skin aging It relates to a composition.

As the largest organ of the human body, which occupies about 16% of the total body volume, the skin is in direct contact with the external environment and is important to protect the body from many deadly harmful factors such as temperature, humidity, and ultraviolet radiation. Act as a shield.

However, as they age, skin cells are damaged by various contaminants, strong ultraviolet rays, stress, and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization. .

Skin aging is largely classified into intrinsic aging and extrinsic aging, which are clinically aging phenomena that occur naturally as the physiological function of the skin gradually decreases with age. It is characterized by decreased elasticity, rough skin, deep wrinkles and pigmentation. Exogenous aging is an aging phenomenon caused by exogenous factors such as ultraviolet rays, reactive oxygen species and stress. Say These factors lead to rapid aging of the skin, resulting in rough wrinkles on the surface of the skin and a significant decrease in elasticity.

Natural aging (endogenous aging) is difficult to artificially control because it is influenced by genetic factors, while external aging is relatively easy because it is influenced by environmental factors. Representative external aging factors include ultraviolet rays, the most prominent external aging phenomenon is wrinkle formation.

Collagen and elastic elastin proteins, which make up most of the dermis (approximately 70-80% of the total dry weight of the skin), are the major proteins produced by fibroblasts, and are involved in the skin's mechanical firmness, tissue binding and elasticity in the dermal layer. do.

Collagen decreases quantitatively with age and with increasing exposure to ultraviolet light. Collagen is classified according to form and structural features, the most well known of which are Type I, II, III and IV. Collagen and elastin, the major structural components of the skin, deteriorate the structure or decrease the amount of biosynthesis due to natural or external stimuli over time, thereby accelerating skin aging.

In vivo, the synthesis and degradation of extracellular matrix such as collagen is properly regulated, but as aging progresses, its synthesis decreases and matrix metalloproteinase (MMP), an enzyme that breaks down collagen, especially collagen. The expression of collagenase (hereinafter referred to as 'MMP-1') and gelatinase (hereafter referred to as 'MMP-2') is promoted to decrease the elasticity of the skin and to form wrinkles. Ultraviolet radiation and free radicals also activate these degrading enzymes.

In order to suppress the skin aging and slow the progress, cosmetic preparations or skin protection agents containing retinol, retinoid, vitamin C, flavonoids and tocopherols, and other skin protection ingredients have been developed, but various symptoms of skin aging have been developed. There is a limiting problem in improving the system at one time.

Therefore, it is an object of the present invention is a combination of mixed herbal extracts and mixed fruit / seed extracts effective in antioxidant activity, wrinkle improvement, skin whitening and skin moisturizing complex skin aging to improve the various symptoms according to skin aging It is to provide a cosmetic composition for improvement.

The above-described object of the present invention is a mixed herbal extract, which is formed by mixing the germinated Seoktaemok extract, chrysanthemum extract, and grain herb extract, and the wooja extract, Chang-ja extract, branch extract, soil extract, algae extract, and male silk etc. It is achieved by providing a composition for improving skin aging comprising a mixed fruit / seed extract formed by mixing at least three selected fruit / seed extracts.

According to a preferred feature of the invention, it comprises 60 to 80% by weight of the mixed herbal extract and 20 to 40% by weight of the mixed fruit / seed extract.

According to a preferred feature of the present invention, the mixed herbal extract is formed by mixing 35 to 50 parts by weight of germinated soybean extract, 25 to 45 parts by weight of chrysanthemum extract and 5 to 20 parts by weight of grain herb.

According to a preferred feature of the invention, the mixed fruit / seed extract is 20 to 25 parts by weight of wooja extract, 20 to 25 parts by weight of changja extract, 10 to 15 parts by weight of branch extract, soil extract 10 to 15 parts by weight, almond extract 10 To 15 parts by weight, 5 to 10 parts by weight of the male thread, etc., and 5 to 8 parts by weight of bovine fruit extract is formed.

According to a preferred feature of the present invention, the mixed herbal extracts are mixed with germinated Seoktaemok, wild chrysanthemum and grain herb, washed and chopped, dried in the shade and then pulverized to prepare a mixed pulverized powder, the total weight of the mixed pulverized powder 5 It is prepared by mixing the distilled water of 8 to 8 times, followed by heat extraction for 2 to 3 hours at a temperature of 80 to 100 ℃.

According to a preferred feature of the present invention, the mixed fruit / seed extract is selected from three or more fruits / seeds from allies, changja, branch, earth and sand, passersby, mansa etc. After preparing the mixed powder, one selected from the group consisting of water, ethanol, methanol, butanol, ethyl acetate, hydrous propylene glycol, hydrous ethanol and hydrous butylene glycol corresponding to 5 to 20 times the volume of the mixed powder After immersing in the above solvent for 3 to 7 days, distilled water of 5 to 8 times the total weight of the mixed powder is mixed and extracted by heating for 2 to 3 hours at a temperature of 80 to 100 ℃ and then filtered.

According to a preferred feature of the present invention, by mixing 60 to 80% by weight of the mixed herbal extract and 20 to 40% by weight of the mixed fruit / seed extract are aged at low temperature at a temperature of 2 to 6 ℃ in a aging furnace for 5 to 10 days It is then prepared by secondary filtration and added to the cosmetic formulation which is formed into one of a lotion, essence, lotion, cream, pack gel, ointment, patch, and spray.

According to a preferred feature of the invention, the mixture of the mixed herbal extract and the mixed fruit / seed extract is added to the cosmetic formulation after being stabilized with nano liposomes, the mixture of the mixed herbal extract and the mixed fruit / seed extract is It is added at 0.1 to 20.0% by weight relative to the total weight of the nanoliposomal stabilizer stabilized with nanoliposomes.

According to the cosmetic composition for skin aging improvement according to the present invention, the germinated Seoktaetae extract, wild chrysanthemum extract, grain herb extract, wooja extract, changja extract, branch extract, soil extract, sperm extract, male yarn etc. Therefore, when added to the cosmetic formulations, as well as anti-oxidant effect is effective in improving skin wrinkles, skin whitening and skin moisturizing, there is an excellent effect that can improve various symptoms due to skin aging.

In addition, when the cosmetic composition for skin aging improvement according to the present invention is stabilized by nanoliposome stabilization technology and added to the cosmetic formulation, the germinated cedar extract, wild chrysanthemum extract, grain herb extract, allium seed extract, changja extract, branch extract, soil extract In addition, the absorption of the active ingredients contained in the almond extract, male and female loin extracts and the core extract may be doubled to improve the skin aging effect.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to the preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the like elements throughout. , And this does not mean that the technical idea and scope of the present invention are limited.

The cosmetic composition for skin aging improvement according to the present invention contains a mixed herbal extract and mixed fruit / seed extract which are effective in antioxidant activity, skin wrinkle improvement, skin whitening and skin moisturizing, thereby improving various symptoms due to skin aging. In order to be able to be, a mixed herbal extract formed by mixing the germinated Seoktaemok extract, wild chrysanthemum extract and grain herb extract, wooja extract, changjaja extract, branch extract, earthworm extract, Namsa etc. It comprises a mixed fruit / seed extract formed by mixing three or more kinds of fruit / seed extract.

Here, the mixed herbal extract is formed by the mixture of germinated moktae extract, chrysanthemum extract and grain herb extract, it is mixed in an amount of 60 to 80% by weight relative to 20 to 40% by weight of the mixed fruit / seed extract.

Seomoktae is a rich source of vitamins B ₁ , B ₂ and calcium, including protein 42.01%, fat 19.41%, carbohydrate 21.12%, fiber 42.29%, ash 5.2%, moisture 6.63%, other tretoran and lisin. It is an excellent food containing nutrients and herbal medicine.

In particular, the extracts from the germinated bovine herb extract contain large amounts of isoflavones and polyphosphates, which have antioxidant, skin cell growth, wrinkle improvement, collagen synthesis promotion, moisturizing effect and Excellent sunscreen effect

When the germinated extract is less than 35 parts by weight, the above-mentioned pharmacological effect is lowered, and when it is used in excess of 50 parts by weight, since it is not balanced with other components, it is 35 to 50 parts by weight based on the total weight of the mixed herbal extracts. It is preferable to mix.

In addition, it is preferable that the germinated bovine seedlings are selected after germination of seedlings after 24 hours of germination and supplied with sufficient water at a temperature of 22 to 37 ° C. to block sunlight to germinate for 24 hours and then dried.

Chrysanthemum indicum L. is a perennial herb of the Asteraceae, which blooms yellow flowers 1 to 1.5 cm in size in wild mountains throughout the country from October to November. It is also called Sanguk (山 菊). Indicum) has been used mainly in headache, zephyr fever, and blood detoxification. The essential ingredients of essential oils, essential oils, linin, luteolin glycosides, chrysanthemin, chrysanthemaxanthin, polysaccharides, coumarins, and infants It contains lactone (Yejuhua lactone), and the main components of essential oils include Comphene, Campo, Carvone, and the like.

The chrysanthemum extract extracted from the chrysanthemum, the flower of the hydrangea, is known for various physiological activities including central nervous sedation, blood pressure lowering effect, inhibitory effect on Mycobacterium tuberculosis bacteria and various viruses, and effect on lung cancer and liver cancer. The effect and UV protection effect are very good.

When the chrysanthemum extract is used less than 25 parts by weight, the above-mentioned pharmacological effect is inferior, and when it is used in excess of 45 parts by weight, the balance with other components is not achieved, so it is 25 to 45 parts by weight based on the total weight of the mixed herbal extracts. It is preferable to mix.

Grain herb (穀 精 草; Eriocaulon sieboldianum SIEB.) Is an annual herb of Eriocaulaceae, and the grain herb extract extracted from the grain has excellent anti-atopic and anti-inflammatory and skin regeneration effects.

If the grain extract is used less than 5 parts by weight of the above-mentioned pharmacological effect, when used in excess of 20 parts by weight is not balanced with other components, mixed 5 to 20 parts by weight relative to the total weight of the herbal extracts It is preferable to be.

The results of experiments on the skin regeneration effect of various herbal extracts including germinated Seoktae extract, wild chrysanthemum extract and grain herb extract are shown in the table below, as shown in the results table, germinated Seoktae extract, wild chrysanthemum extract and grain herb extract The regeneration effect is superior to other herbal extracts.

The mixed herbal extracts are washed, chopped, mixed with germinated Seoktaemok, Chrysanthemum and Grain herb, dried to 0.1-2% moisture content in the shade, and then pulverized to prepare a mixed powder, and then 5 to 8 of the total weight of the mixed powder. Pear distilled water is mixed and extracted by heating for 2 to 3 hours at a temperature of 80 to 100 ℃ it is preferably filtered and stored frozen.

Seomoktae, wild chrysanthemum, grain extract is more economical and effective extraction is possible by the addition of purified water by adding purified water than using an alcohol solvent, purified water is preferably added five times the total weight of the mixed powder. In addition, germinated Seoktaemok, wild chrysanthemum and grain herb must be pulverized to 50 to 100 mesh to be effective for extraction, and when filtered, it is preferable to filter by a filter (filter cloth) of 300 mesh or more, especially 400 mesh.

The germinated seedwood extract, wild chrysanthemum extract and grain herb extract may be mixed according to the mixing ratio after extraction, respectively, but for simplicity of the manufacturing process, it is preferable that the germinated seedwood, wild chrysanthemum and grain herb are mixed and mixed according to the mixing ratio after grinding. Do.

Mixed fruit / seed extract is formed by mixing at least three or more kinds of fruit / seed extracts selected from allium extract, changja extract, branch extract, earthworm extract, algae extract, male hawthorn extract, and necrotic extract. It is mixed in an amount of 20 to 40% by weight based on the mixed herbal extracts.

Arctii Fructus is a fruit of the ally belonging to the family Asteraceae (Compositae) .The pharmacological components include lignin-based artigenin and lignin glycosides Arctiin, fatty oils linolein and oleine, Containing phytosterol, gobosterol, vitamin B ₁ , saponin, coumarin, pectin, lemon acid, malic acid, etc., is known for its excellent anti-inflammatory, antifungal and antioxidant activity. It has been used in various species and lancets.

The allergy is preferably extracted after being immersed in ethanol or the like, and the allergy extract is less than 20 parts by weight of the above-mentioned pharmacological effect, when used in excess of 25 parts by weight is not balanced with other components , 20 to 25 parts by weight relative to the total weight of the mixed fruit / seed extract is preferred.

Xanthii Fructus is the fruit of Xanthium strumarium L. The pharmacological component is Xanthostrumain, iodine salt, saponin, Xanthinin and linolein. It contains acid, oleic acid, saturated fatty acid, palmitic acid, stearic acid, etc., which has anti-inflammatory, analgesic, antibacterial and anti-cancer effects. Especially, it is effective for local inflammation caused by sebum clogging pores, diuretics, antipyretics, analgesics, It is known to be effective for colds, headaches, rhinitis, sinusitis, rheumatism, mainly in the form of plasters, and has been used for eczema, thyroiditis, adenitis, boils, smallpox, and other dermatitis.

Changja is preferably extracted after being immersed in ethanol or the like, and Changja extract is less than 20 parts by weight of the above-mentioned pharmacological effect, when used in excess of 25 parts by weight is not balanced with other ingredients , 20 to 25 parts by weight relative to the total weight of the mixed fruit / seed extract is preferred.

Kochia scoparia is called a dicotyledonous plant of Mingaceae, and the plant is dried and then made a broom, and the seed is used for medicinal purposes. The fruit is called Jija or Cheonduja, or Nak-chuja. It has a cold taste and has no poison, so it takes seeds in August and September, and the seeds are dried in the shade, tonic, genital, edema, chest pain, pain, cure, remedy, redness, gonorrhea, diuresis, cystitis, urine It is used as a medicine for stool, nominal, fruit poisoning, constipation, and has antibacterial effect. The outpost contains pharmacological components such as tannins, flavonoids, coumarins, and saponins, and the fruit contains triterpene glycosides and milk fats, so hepatoprotective, dipharyngeal, glycogen accumulation, anti-lipidism, anti-inflammatory and diuretic effects are excellent. Do.

It is preferable that the branched person is immersed in ethanol, and then extracted by heating, and the branched extract is less than 10 parts by weight of the above-mentioned pharmacological effect, and when used in excess of 15 parts by weight, it is not balanced with other components. It is preferable to mix 10-15 weight part with respect to the total weight of the mixed fruit / seed extract.

Cuscuta Chinensis Lam. Is an annual herbaceous herb of the Convolvulaceae, used as a medicinal herb. It is also used as a herbal medicine.

Tosa has a taste, sweetness and plain properties. It is effective in protecting cancer, heart, blood pressure and lowering blood sugar, kidney and spleen. Traditionally, it is used as a tonic for gastrointestinal tract, oil well and low back pain. It was used as a sedative, painkiller and diarrhea.

Tosa contains p-Coumaric acid, Chlorogenic acid, Cuscutoside A, Cuscotoside B, Amylase, and Provitamin A. It is effective in the treatment and has wrinkle improvement, elasticity improvement, skin moisturizing and soothing effect.

The earth and sand is preferably immersed in ethanol, and then extracted by heating, and the earth and sand extract is less than 10 parts by weight of the above pharmacological effect, if used in excess of 15 parts by weight is not balanced with other components It is preferable to mix 10-15 weight part with respect to the total weight of the mixed fruit / seed extract.

Prunus is the seed of Prunus armeniaca Linne Var. Ansu Maximowicz belonging to the genus Amygdalaceae or other related plant of the same age, widely used in asthma, dyspnea, edema, etc. As a pharmacological ingredient, it contains amygdalin-based, fatty oil-based and various oil-based amino acids, and it has whitening, collagen biosynthesis promoting effect, wrinkle improvement effect, cell regeneration effect, skin shine, skin nutrition, itching removal It is effective in controlling dry skin.

The almond is preferably immersed in ethanol, and then extracted by heating. The almond extract is less than 10 parts by weight of the above-mentioned pharmacological effect, when used in excess of 15 parts by weight is not balanced with other components It is preferable to mix 10-15 weight part with respect to the total weight of the mixed fruit / seed extract.

Namsa-deungsil (南 蛇 藤 實) is the fruit of the genus Nova, belonging to the Celastraceae. The vine stems and roots of Novak vines are called Namsadeung and Namsadeung, respectively, and have been used for musculoskeletal pain, bruises, and various bacterial infections caused by rheumatism.

Male back room is spicy, warm and non-toxic, and in one shot, menstrual pain, rheumatoid arthritis, degenerative arthritis, dizziness and pain in the head, muscle and bone pain, limb paralysis, back and leg pain, lack of energy It is known to be good for dysentery, purulent dermatitis, etc., and contains alkaloids, so it is excellent in tyrosinase expression inhibitory activity and melanin biosynthesis inhibitory activity.

It is preferable that the south yarn is immersed in ethanol or the like and extracted by heating, and the south fruit is less than 5 parts by weight of the above-mentioned pharmacological effect, when used in excess of 10 parts by weight of the balance is made with other ingredients Since it is not supported, it is preferably mixed at 5 to 10 parts by weight based on the total weight of the mixed fruit / seed extract.

Goesil (槐 實) is the fruit of the sophora japonica L., a broad-leaved arborescent tree belonging to the legume plant, and in oriental medicine, it has effects such as tonic, hemostasis, and bleeding. It is used as a medicine for hematuria and enteritis. Due to the high amount of rutin, it has an excellent effect on the repair of damaged blood vessels and has excellent anti-inflammatory and antibacterial effects.

It is preferable that the bovine fruit is immersed in ethanol and then heated and extracted, and the bovine fruit extract is less than the aforementioned pharmacological effect when used in less than 5 parts by weight, and when used in excess of 8 parts by weight, it is not balanced with other components. , 5 to 8 parts by weight based on the total weight of the mixed fruit / seed extract is preferably mixed.

Mixed fruit / seed extract is selected from three kinds of fruits / seeds from allies, changja, branch, earth and sand, pedestrian, mansa etc. To prepare a mixed powder, and then select from the group consisting of water, ethanol, methanol, butanol, ethyl acetate, hydrous propylene glycol, hydrous ethanol and hydrous butylene glycol corresponding to 5 to 20 times the volume of the mixed powder. After immersion in one or more solvents for 3 to 7 days, distilled water of 5 to 8 times the total weight of the mixed powder is mixed and heated and extracted for 2 to 3 hours at a temperature of 80 to 100 ℃ and then filtered.

Purified water is preferably added in 5 times the volume of the mixed powder, and in the case of alcoholic solvent, 5 times or more of the volume of the mixed powder so as to evaporate upon heating extraction for 2 to 3 hours without evaporating at 20 to 30 ° C. for about 10 days. It is preferable that the amount is used. The fruit / seed mixture should be pulverized to 30 to 100 mesh to be effective for extraction, and at the time of filtration, it is preferable to filter over 300 mesh, especially 400 mesh (filter).

Allium extracts, changjaja extracts, branch extracts, earth and sand extracts, almond extracts, male and female thread extracts, and bovine extracts may be mixed according to the mixing ratio after extraction, respectively, but for simplicity of manufacturing process, allies, Changja, branch, earthenware, passersby, passersby, etc. It is preferable that the male and female yarns and the boiled fruit are mixed at the same time according to the mixing ratio after grinding and then simultaneously extracted.

The skin composition for improving skin aging according to the present invention is mixed with 60 to 80% by weight of the mixed herbal extract prepared as described above and 20 to 40% by weight of the mixed fruit / seed extract prepared as described above for 5 to 10 days. Aged at low temperature in a aging furnace at a temperature of 2 to 6 ℃, and then filtered through the secondary known to be added to the cosmetic formulation formed of one of the lotion, essence, lotion, cream, pack gel, ointment, patch, and spray It is preferable to store the reduced pressure concentrated freeze by the frozen decompression concentrate.

In addition, the mixture of the aforementioned herbal extracts and the above-mentioned mixed fruit / seed extracts increases the skin absorption and at the same time stabilizes the active ingredients so that the wrinkle improvement, skin whitening and skin moisturizing effects can be doubled. It is preferably added to a cosmetic formulation which is stabilized with nanoliposomes by a known nanoliposomal stabilization technique, and then formed into one of a lotion, essence, lotion, cream, pack gel, ointment, patch, and spray.

Nanoliposomes applied to the cosmetic composition for improving skin aging according to the present invention have an average particle diameter of 30 to 80 nm and are made of a mixture containing polyols, surfactants, phospholipids, fatty acids and water, of which polyols are all 50-70% by weight of the nanoliposome of is selected from the group consisting of polyol propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propanediol, isoprene glycol, pentylene glycol, erythritol, ziitol and sorbitol It is preferable that glycerin is 40 to 50 weight%. Glycerin has a great effect of adding moisturizing power, but when the glycerin is added too much, the texture may be degraded due to viscosity, so it should not exceed 50% by weight.

The surfactant applied to the nanoliposomal stabilization technique is preferably a nonionic surfactant containing an alkoxylated alkyl ether, an alkoxylated alkyl ester, an alkyl polyglycoside, a polyglyceryl ester or a sugar ester, and the like. It is preferable that the herbal extract and the mixture of the aforementioned mixed fruit / seed extract are added at 0.5 to 5.0% by weight based on the total weight of the nanoliposome stabilizer stabilized with nanoliposomes.

Phospholipids applied to the nanoliposomal stabilization technology is natural lecithin, and the content of the phospholipid is 2 to 8% by weight based on the total weight of the nanoliposomal stabilizer in which the mixture of the above-mentioned mixed herbal extract and the above-mentioned mixed fruit / seed extract are stabilized with nanoliposomes. It is preferable that it is%.

 The fatty acid to be applied to the nanoliposome stabilization technique is a saturated or unsaturated fatty acid such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid or linoleic acid, and the content of fatty acid is the above-mentioned mixed herbal extract and the above-mentioned mixture. It is preferred that the fruit / seed extract mixture is 0.1 to 1.0% by weight relative to the total weight of the nanoliposome stabilizer stabilized with nanoliposomes.

The water used in the nanoliposomal stabilization technique is generally deionized distilled water, and the amount of the liposome stabilizer is 5% based on the total weight of the nanoliposome stabilizer in which the mixture of the aforementioned herbal extracts and the above-mentioned mixed fruit / seed extracts is stabilized with nanoliposomes. It is preferable that it is to 25 weight%.

The mixture of the above-mentioned mixed herbal extract and the above-mentioned mixed fruit / seed extract is preferably 0.1 to 20% by weight, in particular 15 to 20% by weight, based on the total weight of the nanoliposome stabilized nanoliposomes, but less than 15% by weight. When the amount is added, there is a problem that the skin wrinkle improvement, skin whitening and skin moisturizing effect is somewhat reduced, and when the amount exceeds 20% by weight, there is a problem in the stability of the nano liposomes and the additional effect is not large, which is uneconomical problem.

Example  One

After mixing 45 parts by weight of germinated soybean paste, 40 parts by weight of chrysanthemum, 15 parts by weight of grain currant, and washing and slicing, it is sufficiently re-dried in the shade and pulverized to 75 mesh. After heat extraction for 3 hours at, it was filtered through a 400 mesh filter (filter) to prepare a mixed extract extracted and then stored frozen.

In addition, after mixing 25 parts by weight of allium extract, 22 parts by weight of Changjaja, 13 parts by weight of branch extract, 13 parts by weight of earthenware, 13 parts by weight of algae, 8 parts by weight of male yarn, 8 parts by weight of silk yarn, and 6 parts by weight of silkworm, After sufficiently redrying, the mixture was pulverized to 75 mesh, immersed in 5 volumes of ethanol for 3 days, and then 5 times purified water was added and extracted by heating at 100 ° C. for 3 hours or more, followed by a 400 mesh filter (filter). Filtration was carried out to prepare a mixed fruit / seed extract and then frozen.

60 parts by weight of the mixed herbal extracts prepared as described above and 40 parts by weight of the mixed fruit / seed extract were mixed one week before use as a cosmetic, and aged at low temperature at 4 ° C. in a aging furnace for one week, followed by a filter of 0.45 um. After filtration, the mixture was concentrated under reduced pressure using a freeze pressure reducer and frozen.

The total ratio of the composition of Example 1 described above is shown in Table 1 below.

division  Ratio (% by weight) Remarks Germinated Seoktaetae Extract 27 Hydrothermal extraction Chrysanthemum Extract 24 Hydrothermal extraction Grain Extract 9 Hydrothermal extraction Allies Extract 10 Heating after immersion Changi Ja Extract 8.8 Heating after immersion Branch Extract 5.2 Heating after immersion Tosa Extract 5.2 Heating after immersion Almond extract 5.2 Heating after immersion Southern lamp thread extract 3.2 Heating after immersion Gochu extract 2.4 Heating after immersion

Example  2

The cosmetic composition for improving skin aging according to the present invention, prepared as in Example 1, is an example of a nanoliposome stabilizer stabilized with nanoliposomes using a high pressure homogenizer.

The cosmetic composition for improving skin aging according to the present invention is 20% by weight based on the total weight of the nanoliposome stabilizer and is purified as shown in Table 2 including purified water (ionized water).

division Detailed composition Composition ratio (% by weight) Remarks According to the invention
Skin aging improvement
Cosmetic composition (A)
See Example 1
20



Nanoliposomes (B) Polyol
(60% of B + C) glycerin 24


80% by weight of nanoliposome
(Including purified water) Polyol Propylene Glycol 24 Surfactants
(4% of B + C) Polyglyceryl esters
(Nonionic) 3.2 Phospholipids
(7% of B + C) Lecithin (from soybeans) 5.6 fatty acid
(1% of B + C) Lauric acid, linolenic acid 0.8 Etc Ceramide (natural) 0.4 sub Total 58 Purified water (C) Remaining quantity (22) system 100

First, after dissolving the nanoliposome (B) composition at a temperature of 75 ℃ and mixed with a general homogenizer for at least 10 minutes, and emulsified again for at least 10 minutes while adding purified water (C), according to the present invention After mixing the cosmetic composition (A) for skin aging improvement three times to make a general emulsion phase, the whole is re-destructed under pressure of 1,000 bar or more in a high-pressure homogenizer, and the first pass is completed homogeneously. At this time, by taking a sample and passing the high-pressure homogenizer 3 to 5 times under the same pressure according to the number of times determined by passing the nanoliposomal stabilizer stabilized by the skin aging improvement cosmetic composition according to the present invention with nanoliposomes This is the final completion.

Example  3

Using the nanoliposome stabilizer prepared in Example 2, a lotion, which is a kind of cosmetic for improving skin aging, could be prepared as shown in Table 3 below.

Manufacturing example One 2 3 4 According to the invention
Skin aging improvement
Cosmetic composition (A)
-
-
-
25 Stabilized with Nanoliposomes
Nanoliposomal Stabilizers
(A + B + C)
20
2.0
-
- Stearyl alcohol 0.5 0.5 0.5 0.5 Cetosteayl alcohol 1.5 1.5 1.5 1.5 Glyceryl Stearate 3.0 3.0 3.0 3.0 Triglyceride 2.0 2.0 2.0 2.0 ethanol 5.0 5.0 5.0 5.0 Dimethicone 3.0 3.0 3.0 3.0 Cetearyl Olate 1.2 1.2 1.2 1.2 Sorbitan olivate 1.5 1.5 1.5 1.5 Sodium hyaluronate 2.5 2.5 2.5 2.5 Methyl Soyate 1.5 1.5 1.5 1.5 Butylene Glycol 2.0 2.0 2.0 2.0 Hydrogenated
Olive oil 5.0 5.0 5.0 5.0 Ascorbic Acid 1.0 1.0 1.0 1.0 Evening Primrose Oil 1.5 1.5 1.5 1.5 Caprylyl Glycol
(Natural preservation) 1.8 1.8 1.8 1.8 Tocopherol Acetate 0.5 0.5 0.5 0.5 Squalane 5.0 5.0 5.0 5.0 Liquid paraffin 2.0 2.0 2.0 2.0 Trioctanoine 2.0 2.0 2.0 2.0 Allantoin 1.5 1.5 1.5 1.5 Betaine 3.0 3.0 3.0 3.0 Spices (natural) Quantity Quantity Quantity Quantity Purified water Remaining amount (TO 100) Remaining amount (TO 100) Remaining amount (TO 100) Remaining amount (TO 100)

Preparation Example 1 is prepared by adding a cosmetic composition for improving skin aging according to the present invention (A) by adding a liposome stabilizer (A + B + C) stabilized with nanoliposomes at a total of 20% by weight of the lotion. Example 2 is prepared by adding a cosmetic composition for improving skin aging (A) with a nanoliposome stabilizer (A + B + C) stabilized with nanoliposomes in an amount of 2% by weight of the whole lotion, and Preparation Example 3 according to the present invention. It is a lotion containing no cosmetic composition (A) for skin aging improvement, and Preparation Example 4 is added to 25% by weight of the whole lotion as it is without stabilizing the skin composition for improving skin aging according to the present invention (A) with nanoliposomes. It is manufactured by.

Example  4

In order to find out whether the cosmetic composition for skin aging improvement according to the present invention has an anti-wrinkle effect, the effect on the proliferation of skin cells was analyzed by treating the skin cells by concentration. Specifically, human fibroblasts (HNF, Normal Human Fibroblast) (CCD-986K, KCLB 21947) were incubated in a T-75 flask, washed twice with 1 × PBS, and treated with 0.025% Trypsin-EDTA to attach to the flask. Dropped cells. After the cells were obtained, the cells were centrifuged at 1500 rpm for 7 minutes (Heraeus, Labofuge 400R), and then pelleted cells were suspended in 10 ml of DMEM (including 5% FBS). After measuring the number of cells by using a hematocytometer (Heamacytometer), the cells of about 2 × 103 was dispensed into each well of 200 well each 96 well plate. When the cells grow to about 40% of the well area, the composition of the present invention is added to each well at concentrations of 0.1, 1, and 10 ppm while exchanging with fresh DMEM (containing 2% FBS) medium. Cells were cultured in CO ₂ .

MTT <3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide> for measuring the regenerative effect of cells after 3 days of culture by adding the cosmetic composition for improving skin aging according to the present invention Analyzes were made. MTT (Sigma) was dissolved in PBS to a concentration of 2 mg / ml, and then treated with 10% (20 μl) of the cell culture medium, and the cells were further incubated for 3 hours. After discarding the supernatant of the cultured cells, add 150 μl of DMSO and mix well or add 100 μl of 0.04 N HCl (in acid-isopropanol) and mix. After the procedure, the absorbance was measured at 560 nm using an ELISA reader (μQuant, Bio-Tek instrument INC.) (Reference: 650 nm).

As a result, when the absorbance value of the cells cultured without adding the cosmetic composition for improving skin aging according to the present invention was 100% (that is, cell proliferation was 100%), the cosmetic composition for skin aging improvement according to the present invention. When added, the fibroblast proliferation effect was 153 to 352% at a concentration of 0.1 to 10 ppm.

It can be seen that the individual extract is a very good proliferation effect compared to the proliferation effect of 125 to 291% at a concentration of 0.1 to 10 ppm.

Also, as shown in Table 4, as the concentration of the cosmetic composition for improving skin aging according to the present invention was increased, the skin regeneration effect was gradually increased, showing a stable characteristic.

division ppm Skin regeneration effect Skin aging according to the present invention
Cosmetic compositions for improvement
No addition
100 Addition of cosmetic composition for skin aging improvement according to the present invention One 193 10 352 Eoseongcho
(Individual) One 126 10 128 Germinated
(Individual) One 291 10 189 Chrysanthemum
(Individual) One 125 10 258 Grain corner
(Individual) One 131 10 134

Example  5

In order to confirm that the cosmetic composition for improving skin aging according to the present invention exhibits a moisturizing effect, hyaluronic acid, which is a representative moisturizer, was compared with the moisturizing effect. Specifically, 0.5% of the composition prepared by Example 1 and 0.5% of hyaluronic acid (hyaluronic acid) were applied to the inside of the arm of eight testers and taped. The degree of skin hydration was analyzed as an average value by measuring conductance before and after the sample treatment, immediately after the treatment, and after 2 hours of treatment using a coronometer.

As a result, in case of hyaluronic acid, the skin moisturizing rate was 35% immediately after treatment but decreased to 5% after 2 hours of treatment, and when the composition of the present invention was treated, the skin moisturization rate was 33% immediately after treatment, but after 5 hours of treatment. It was found to decrease to%, showing a similar effect as a moisturizer.

Example  6

20 skin irritation tests were selected as testers and conducted according to known skin irritation test criteria. The results (average value) corresponded to no stimulus as shown in Table 5 below, and found that it can be used as a cosmetic.

Test substance Test concentration (%) P.I.I Judgment Remarks According to the invention
Skin aging improvement
Cosmetic composition
10
0.188
Non-irritating
Available

Example  7

For Preparation Examples 1, 2, and 4 of Example 3, the sample material was observed in a constant temperature bath at -5 ° C, 5 ° C, 30 ° C, 37 ° C, and 45 ° C for 3 months to observe changes in appearance, liquid phase separation, and degree of suspension. As a result of evaluating the storage stability according to the temperature and time, it was confirmed that the storage stability up to three months in the temperature range of -5 ℃ to 45 ℃ as a whole.

Claims (8)

Mixed herbal extracts formed by mixing germinated Seoktaemok extract, wild chrysanthemum extract and grain herb extract; And
A composition for improving skin aging, comprising a mixed fruit / seed extract formed by mixing at least three kinds of extracts selected from allium extracts, changja extracts, branch extracts, earthworm extracts, algae extracts, and sperm extracts. The method according to claim 1,
60 to 80% by weight of the mixed herbal extract and 20 to 40% by weight of the mixed fruit / seed extract. The method according to claim 1,
The mixed herbal extract is a cosmetic composition for improving skin aging, characterized in that the germinated moth extract 35 to 50 parts by weight, wild chrysanthemum extract 25 to 45 parts by weight and 5 to 20 parts by weight of grain herb. The method according to claim 1,
The mixed fruit / seed extract is 20 to 25 parts by weight of allium extract, 20 to 25 parts by weight of Changja extract, 10 to 15 parts by weight of branch extract, 10 to 15 parts by weight of earth and sand extract, 10 to 15 parts by weight of algae extract, Namsa etc. 5 to 10 parts by weight and 5 to 8 parts by weight of the bovine extract is a cosmetic composition for improving skin aging, characterized in that formed. The method according to claim 1,
The mixed herbal extract is washed, chopped, mixed with germinated Seoktaemok, wild chrysanthemum and grain herb, dried in the shade and then pulverized to prepare a mixed powder, and then mixed with distilled water of 5 to 8 times the total weight of the mixed powder. A skin composition for improving skin aging, which is prepared by heating and extracting for 2 to 3 hours at a temperature of 80 to 100 ℃. The method according to claim 1,
The mixed fruit / seed extract is selected from three or more fruits / seeds from allies, Chang-ja, branch, earth and sand, passersby, mansa etc. fruit and seeds through a washing process and then dried in the shade to prepare a mixed powder Then, 3 to 1 or more solvents selected from the group consisting of water, ethanol, methanol, butanol, ethyl acetate, hydrous propylene glycol, hydrous ethanol and hydrous butylene glycol corresponding to 5 to 20 times the volume of the mixed powder. After immersion for 7 days, 5 to 8 times the total weight of the mixed powder, distilled water is mixed and extracted by heating and extraction for 2 to 3 hours at a temperature of 80 to 100 ℃ after filtration, characterized in that the cosmetic composition for skin aging improvement . The method according to claim 1,
60 to 80% by weight of the mixed herbal extracts and 20 to 40% by weight of the mixed fruit / seed extract were mixed at low temperature in a aging furnace at a temperature of 2 to 6 ° C. for 5 to 10 days, followed by secondary filtration. After the skin lotion, essence, lotion, cream, pack gel, ointment, patch, and a cosmetic composition for improving skin aging, characterized in that it is added to the cosmetic formulation is formed as one of the spray. The method of claim 7,
The mixed herbal extract and the mixture of the mixed fruit / seed extract are stabilized with nanoliposomes and then added to the cosmetic formulation, and the mixed herbal extract and the mixture of the mixed fruit / seed extract are stabilized with the nanoliposomes stabilized. A skin composition for improving skin aging, characterized in that added to 0.1 to 20.0% by weight based on the total weight of the cargo.