KR20090080714A - Apparatus and Method of Automatic Cell injection - Google Patents

Abstract

An apparatus of automatic injection to a cell is provided to automatically inject certain component to various size of cells using reaction force generated when inserting the apparatus. An apparatus(100) comprises a fluid system(200), counter(300), tong device(400), injection system(500) and power measuring system(504). The fluid system moves a cell. The counter is installed at one side of fluid system, and have the information on movement and position of the cell. The fixing device is installed at one side of fluid system and affixes the position of the cell. The injection system comprises an injector which is used in injecting certain material into the fixed cell. The apparatus further comprises a power input unit(602) and control unit(601). A method for injecting to cell comprises: a step of obtaining the information of cell position using the counter when the cell moves through the fluid system; a step of fixing the cell using the fixing device based on the position information; a step of inserting the injector to the fixed cell and a step of injecting the component to the cell.

Description

Automatic cell injection device and cell injection method {Apparatus and Method of Automatic Cell injection}

The present invention relates to an automated device and an injection method for automatically injecting a predetermined substance into cells of various sizes moving in a fluid by using a reaction force generated when inserting an injector into a cell.

Hereinafter, a description will be briefly given of techniques generally used in connection with the technical field of the present invention.

In general, as a method for injecting cells into a living tissue, there is a method of injecting the fixed tissue with a micro vibrating injection needle to inject cell suspension into the tissue through the needle. In addition, there is a method of injecting stem cells using a catheter through blood vessels connected to the organs of the living body.

On the other hand, as a commonly used injection method, the technique of injecting a predetermined substance into the cell itself is not automated, there is a problem that requires a lot of manual work of the operator. In addition, in the case of injecting a predetermined substance into the cell, the success rate of injecting the predetermined substance is also determined by the ability of the operator, so a new type of injection automation system is required.

In addition, it is difficult to implement a method of injecting a predetermined substance into each cell using general techniques and an automated device for injecting a predetermined substance into each cell. This is because general techniques relate to a method or device for injecting multiple cells into a particular biological tissue or organ.

The present invention has been made to solve the problems of the general technique as described above, an object of the present invention is to provide an apparatus and method for automatically injecting a predetermined substance into each cell.

It is another object of the present invention to provide an apparatus and method for injecting a substance into a cell by using the reaction force generated when the injector is inserted to inject the substance into the cell.

In order to solve the above technical problem, embodiments of the present invention are directed to an automated device and an injection method capable of automatically injecting a predetermined substance into cells of various sizes flowing in a fluid.

In one aspect of the present invention, in the cell injection device for injecting a predetermined substance into the cell by using the reaction force of the cell, the cell injection device is provided on one side of the fluid system and the fluid system for moving the cell movement and One side of the injection system and an injection system comprising a counter for acquiring position information and a forceps device which is provided on one side of the fluid system and an injector used to inject a predetermined substance into the fixed cell. It may include a force measuring system that can be provided to measure the reaction force generated when the injector is inserted into the cell.

The cell injection device recognizes the position of the cell through a power input unit and a counter for supplying power, drives the forceps according to the information on the position of the cell, and uses a force measuring system to inject a predetermined substance into the cell. The control unit may further include a control unit.

The fluid system includes a cell tunnel including a first cell reservoir for storing the cells and a fluid for moving the cells before injecting a substance into the cell and an injector inlet for inserting an injector into the cell tunnel. And a second cell storage unit in which cells are stored after the predetermined substance is injected. At this time, the fluid system may be generated using silicon.

In addition, the counter may include a transmitter for transmitting a signal for recognizing movement and position of the cell and a receiver for receiving a signal transmitted from the transmitter. In this case, the transmitter may generate a laser signal or an electromagnetic signal in a direction in which the receiver is located.

The injection system includes an injector for injecting a predetermined substance into a cell fixed in the fluid system through an injector inlet provided at one side of the fluid system, and an injection material storage unit provided at one side of the injector to store a predetermined substance to be injected into the cell. And a driving unit for driving the injector to inject a predetermined substance into the cell.

In another aspect of the present invention, a cell injection device for injecting a predetermined substance into a cell using the reaction force of the cell is provided on one side of the fluid system and the fluid system for moving the cell to obtain information about the movement and position of the cell It is provided on one side of the counter and the fluid system, and when a predetermined force is applied, the shape is deformed in a direction in which the predetermined force is applied, and the forceps device including a piezoelectric material for fixing the cell at a predetermined position is fixed to the fixed cell. It may include an injection system including an injector used to inject a substance and a force measuring system provided on one side of the injection system to measure the reaction force generated when the injector is inserted into the cell.

In another aspect of the present invention, a cell injection device for injecting a predetermined substance into the cell by using the reaction force of the cell is provided on one side of the fluid system and the fluid system for moving the cell to provide information about the movement and position of the cell An injection device, which is provided on one side of the counter and the fluid system to obtain, and includes a forceps device to fix the cells by using dielectric electrophoresis generated when an alternating voltage is applied, and an injector used to inject a predetermined substance into the fixed cells. It may include a force measuring system provided on one side of the system and the injection system to measure the reaction force generated when the injector is inserted into the cell.

 As another aspect of the invention, the cell injection method for injecting a predetermined substance into the cell using the reaction force of the cell, the step and position of obtaining the position information of the cell using a counter when the cell moves through the fluid system Based on the information, information about reaction force generated when the cell is fixed by using a forceps device, a step of inserting the injector included in the injection system into the fixed cell, and the insertion of the injector into the cell using the force measurement system are provided. It may include the step of injecting a predetermined material through the injector to the cell based on the information on the measuring and the reaction force.

In this case, the location information may include information about the movement of the cell and a specific location of the cell.

In addition, the cell injection method may further include applying a pressure to the fluid system to move the cell through the fluid system before obtaining the position information. In addition, after injecting the predetermined material, the method may further include removing the injector using a driver included in the injection system.

In still another aspect of the present invention, a cell injection method for injecting a predetermined substance into a cell by using a reaction force of the cell comprises the steps of acquiring position information of the cell using a counter when the cell moves through the fluid system; When the predetermined force is applied, the shape is changed in the direction in which the predetermined force is applied, and the cell is fixed according to the position information by using a forceps device including a piezoelectric material that fixes the cell at a predetermined position. Inserting the injector included in the injection system into the cell, and measuring information on reaction force generated when the injector is inserted into the cell by using a force measuring system; Injecting the substance.

In still another aspect of the present invention, a cell injection method for injecting a predetermined substance into a cell by using a reaction force of the cell comprises the steps of acquiring position information of the cell using a counter when the cell moves through the fluid system; Fixing the cells according to the location information and using the forceps device that is provided on one side of the fluid system, using a forceps device to fix the cells using dielectric electrophoresis generated when applying an alternating voltage, and included in the fixed cells in the injection system Measuring the reaction force generated when the injector is inserted into the cell using a force measuring system, and injecting a predetermined substance into the cell through the injector based on the reaction force information. It may include.

According to the present invention has the following effects.

First, the present invention can provide a cell injection system capable of automatically injecting a predetermined substance into a cell by using a fluid system and a counter, a forceps device, an injection system, and a force measurement system.

Second, when the injection method of injecting a predetermined substance into the cell by hand, a long time practice time is required, and the success rate depends on the skill and fatigue of the operator. In order to compensate for this, in the present invention, the cell injector automatically captures the moment of injecting a predetermined substance into the cell by using the reaction force generated when the injector is inserted into the cell, thereby automatically injecting the predetermined substance into the cell. It can be done with

In order to solve the above technical problem, the present invention provides an automatic device and an injection method that can automatically inject a predetermined material into cells of various sizes flowing in the fluid by using the reaction force generated when the injector is inserted into the cell It is about.

The following embodiments combine the components and features of the present invention in a predetermined form. Each component or feature may be considered to be optional unless otherwise stated. Each component or feature may be embodied in a form that is not combined with other components or features. In addition, some components and / or features may be combined to form an embodiment of the present invention. The order of the operations described in the embodiments of the present invention may be changed. Some components or features of one embodiment may be included in another embodiment or may be replaced with corresponding components or features of another embodiment.

In the drawings, the thickness of layers, films, panels, regions, etc., are exaggerated for clarity. Like reference numerals designate like parts throughout the specification. When a part of a layer, film, area, plate, etc. is said to be "on top" of another part, this includes not only the other part being "right over" but also another part in the middle. On the contrary, when a part is 'just above' another part, there is no other part in the middle.

Specific terms used in the following description are provided to help the understanding of the present invention, and the use of the specific terms may be modified in other forms without departing from the technical spirit of the present invention.

Among the terms used in the embodiments of the present invention, a device for automatically injecting a predetermined substance into a cell is called a 'cell injection device', and a method of automatically injecting a predetermined substance into a cell is a 'cell injection method'. Let's call it.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a view showing a block diagram (a) and a perspective view (b) of a cell injector according to an embodiment of the present invention.

Referring to Figure 1 (a), the cell injection device 100 is a component, the fluid system 200 for moving the cells in a predetermined fluid, the counter 300 for obtaining information about the movement and position of the cells Reaction force generated when the injector is inserted into the cell, provided with a forceps device 400 for fixing the cells moving in the fluid, an injection system 500 for inserting the injector into the fixed cells and the injection system is inserted into the cell And a force measuring system 504 capable of measuring. In addition, the cell injection device 100 may further include a control unit 601 and a power input unit 602 for supplying power to each component.

At this time, the control unit 601 operates the forceps device 400 by using the information on the flow and position of the cells obtained through the counter 300, the predetermined material in the cells using the force measurement system 504 It controls to inject.

Referring to FIG. 1B, the cell injection device 100 includes a fluid system 200. The fluid system 200 includes a first cell storage unit 201 where cells are initially stored, a cell tunnel 202 through which cells move through a fluid, an injector inlet 203 into which an injector is inserted, and a predetermined material. It may include a second cell storage unit 204 in which the cells are stored.

One side of the fluid system 200 may be provided with a counter 300, a forceps device 400, an injection system 500 and a force measurement system 504. In addition, the cell injection device 100 is a control unit 601 and the cell to control the fluid system 200, the counter 300, the forceps device 400, the injection system 500 and the force measurement system 504 on the plate It may further include a power input unit 602 for supplying power to the injection device 100.

The shape of the cell injection device 100 shown in Figure 1 (b) is only one example for showing a preferred embodiment of the present invention. Therefore, any type of cell injection device is included in the technical idea of the present invention as long as the configuration of FIG. 1 (a) is included.

In addition, in Figure 1 (b) it is shown in a simplified geometric figure to clearly show the shape of each component. Accordingly, each component of FIG. 1 (b) may be modified into a preferred form in an actual implementation.

2 is a view showing a fluid system 200 included in the cell injection device 100, according to an embodiment of the present invention.

Referring to FIG. 2, the fluid system 200 is provided at one side of the first cell storage unit 201 and the first cell storage unit 201 in which the original cells 205a are stored to provide the first cells 205a. A cell tunnel 202 containing a suitable solution for movement, an injector inlet 203 formed at one side of the cell tunnel 202 to form a passage into which an injector included in the injection system is inserted, and a cell into which a predetermined substance is injected It may include a second storage unit 204 is stored (205b).

In order to inject a predetermined substance into the initial cells 205a stored in the first cell storage unit 201 of the fluid system 200, the second cell storage unit 204 in the first cell storage unit 201. Initial cells must be moved. Therefore, when pressure is applied to the first cell storage unit 201 using the power supplied from the power supply unit 602, the initial cells 205a stored in the first cell storage unit 201 are stored in the cell tunnel 202. Will go through. At this time, the pressure applied to the first cell reservoir is sufficient to require the movement of the small cells. In the exemplary embodiment of the present invention, the pumping of the cells by applying pressure to the first cell storage unit 201 is exemplified, but the cells may be moved using mechanical energy.

The fluid system 200 may be manufactured using various kinds of materials. In the exemplary embodiment of the present invention, a fluid system is preferably created using silicon (Si) as an example. Fabricating the fluid system with silicon has the advantage that the operator can easily observe the movement of the cells.

In addition, the height h of the injector inlet 203 in the fluid system 200 may be determined by the pressure P applied to the fluid (or solution) and the density δ of the fluid.

3 is a diagram illustrating a counter 300 capable of acquiring information about the movement and position of the cell 205, according to an exemplary embodiment of the present invention.

Referring to FIG. 3, the counter 300 may include a transmitter 301 for transmitting a signal for recognizing a movement and a location of a cell, and a receiver 302 for receiving a signal transmitted from the transmitter.

Information about the position of the cell 205a may be obtained by using the disconnection of the signals of the transmitter 301 and the receiver 302. For example, while the transmitter 301 of the counter 300 continuously transmits a signal to the receiver 302, when the cell 205a moves between the transmitter 301 and the receiver 302, the signal is cut off. . The counter 300 may obtain information about the position of the cell 205a by using the disconnection of the signal.

In an embodiment of the present invention, a signal of light may be used as a signal generated by the transmitter. That is, the transmitter 301 may be a light emitter and the receiver 302 may be a light receiver. The light emitting unit 301 continuously generates a laser signal and recognizes the position of the cell by using the moment when the laser signal is cut off as the cell 205a passes. Of course, a signal such as electromagnetic waves or sound waves may be used as the signal generated by the transmitter.

At this time, when the position of the cell is recognized by the counter 300, the pressure applied to the first storage unit 201 may be stopped to prevent the movement of the next cell. At this time, by using the information on the distance (d) and the flow rate (v) already known in the forceps device 400 can be fixed to the cell at the desired position.

The counter 300 shown in FIG. 3 may take various forms. Therefore, if the counter 300 is provided on one side of the cell tunnel 202, and the signal transmitted from the transmitter 301 can be transmitted to the receiver 302 through the cell tunnel 202, the counter 300 It may take any form.

4 is a view showing the principle of operation of the forceps device 400 and the forceps used to hold cells of various sizes moving in the fluid system 200 in a predetermined position, according to one preferred embodiment of the present invention. to be.

Figure 4 (a) shows a forceps device 400 using a piezoelectric material (400a) to fix the cells, Figure 4b shows the piezoelectric material (400a) is deformed when the electric field is applied to the piezoelectric material (400a) to fix the cells It is a figure which shows a state to make.

In FIG. 4A, the piezoelectric material 400a refers to a material in which charge polarization occurs due to mechanical deformation or deformation occurs when an electric field is applied. This phenomenon is also called a piezoelectricity phenomenon. At this time, materials that are well formed piezoelectric phenomenon are called piezoelectric materials. Representative examples of the piezoelectric materials include quartz, tourmaline and Rochelle salt, and various piezoelectric materials may be artificially manufactured.

Referring to FIG. 4A, when pressure is applied to the first storage unit 201, the initial cells 205a sequentially move to the second storage unit 204. In this case, when the information on the movement and position of the cell is acquired by the counter 300, the pressure applied to the first storage unit 201 may be stopped for movement of the cell after a predetermined time. Accordingly, the movement of the cells is stopped, and when the cells are positioned on the piezoelectric material 400a, the piezoelectric material 400a is operated to fix the cells 205a as shown in FIG. 4 (b).

5 is another view showing the principle of operation of the forceps device 400 and the forceps used to hold cells of various sizes moving in the fluid system 200 in a predetermined position, in accordance with one preferred embodiment of the present invention. to be.

5 (a) shows a forceps device 400 that uses electrodes 400b to fix cells. Referring to FIG. 5A, when pressure is applied to the first storage unit 201, the initial cells 205a sequentially move to the second storage unit 204. In this case, when the information on the movement and position of the cell is acquired by the counter 300, the pressure applied to the first storage unit 201 may be stopped for the movement of the cell after a predetermined time. Therefore, when the movement of the cell is stopped and the cell is positioned around the electrode 400b, when the AC voltage is applied to the electrode 400b, the cell 205a can be fixed as shown in FIG. 5 (b).

5 (b) shows a state in which cells are fixed by using a dielectric electrophoretic force generated by the alternating voltage when an alternating voltage is applied to the electrode 400b. Referring to FIG. 5B, when the position of the cell is recognized through the counter 300, the pressure applied to move the cell is stopped after a predetermined time. When the cell moves around the electrode 400b of the forceps 400, when an alternating voltage is applied to the electrode 400b, a dielectric electrophoretic force is generated at the electrode to fix the cell.

4 to 5, a method of fixing a cell using a forceps device including a piezoelectric material 400a or an electrode 400b has been described. That is, the user can effectively fix the cells using the forceps device at the desired position, insert the injector and inject a predetermined substance.

6 is a diagram illustrating an example of an injection system 500 and an operation process for injecting a predetermined substance into fixed cells, according to an exemplary embodiment of the present invention.

In FIG. 6A, the injection system 500 includes an injector 501 that penetrates an outer wall of a cell and injects a predetermined injection material into a cell, and an injection material storage unit 502 that stores a predetermined material injected into a cell. And a driver 503 for precisely operating the injector to inject a predetermined substance into the cell and a force measuring system 504 capable of measuring in real time the reaction force generated when the injector is injected into the cell. Can be.

6 (a) and 6 (b) the principle of injecting a predetermined substance into the cells is as follows. When pressure is applied to the outer wall of a cell using a specific substance, reaction forces are generated on the outer wall of the cell. The user may capture the moment when the outer wall of the cell is pierced by using the reaction force.

7 is a view showing the relationship between the reaction force and the distance that can be used in a preferred embodiment of the present invention.

In Figure 7, the vertical axis represents the reaction force and the horizontal axis represents the relationship of the insertion depth from the surface of the cell (Insertion depth). Referring to FIG. 7, the reaction force and the distance increase at a constant rate, and at a certain moment, the reaction force suddenly decreases rapidly. This indicates the position of the moment when the outer wall of the cell is opened by continuously applying a constant force from the moment when the injector contacts the outer wall of the cell. Thus, using FIG. 7, information about the moment when the outer wall of the cell is pierced, that is, the time when a predetermined substance is injected into the cell, may be obtained.

Referring back to FIG. 6B, cells move from the first cell reservoir 201 to the second cell reservoir 204 through the cell tunnel 202. When the counter 300 installed at the upper part of the cell tunnel 202 obtains information about the movement and location of the cells and transfers the information to the controller 601, the controller 601 stores the first cell storage unit after a predetermined time has elapsed. The pressure applied to 201 can be stopped. In addition, when the cell stops on the electrode 400b which is the forceps 400, the controller 601 may apply an AC voltage to the electrode 400b to fix the cell.

However, in order to clearly show how the injector is inserted into the cell in FIG. 6 (b), the shape of the forceps under the injector is not shown. In FIG. 6, the electrode is used as a forceps device as an example, but a piezoelectric material or a material capable of fixing other cells may be used.

The controller 602 may drive the driver 503 to insert the injector 501 into the cell through the injector inlet 203. The moment the injector 501 contacts the outer wall of the cell, the force measurement system 504 can measure in real time the reaction force occurring during insertion of the injector 501 into the cell. In addition, the force measurement system 504 can capture information about the time when a given substance can be injected into the cell according to the measured reaction force. The captured time information is transmitted to the controller 601 to inject a predetermined substance into the cell through the injector 501.

That is, using the method described with reference to FIG. 6, the user can fix the cell and automatically inject a predetermined substance into the cell by a simple operation.

8 is a view showing a process of performing a cell injection method according to an embodiment of the present invention.

Referring to Figure 8, the control unit transmits a start signal indicating the start of the cell injection method to the cell injection device (S801).

Power is supplied from the power supply 602 to the fluid system 200 according to the start signal. When pressure is applied to the first cell reservoir 201 of the fluid system 200, the cells stored in the first cell reservoir 201 are sequentially moved to the cell tunnel 202. At this time, the counter 300 provided on the upper side of the cell tunnel can obtain information about the movement and location of the cell. A process of obtaining cell location information may refer to FIG. 3 (S802).

Information about the movement and position of the cells obtained in step S802 is transmitted to the control unit, the control unit can be fixed to the cell at the position to insert the injector into the cell using the forceps according to the position information (S803). .

The injection system 500 may insert the injector 501 into the fixed cell 205a through the injector inlet 203. At this time, the reaction force generated when the injector 501 is inserted into the cell may be measured using the force measuring device 504. At this time, the principle of measuring the reaction force can refer to the description of Figure 6 and 7 (S804).

Accordingly, the controller 601 may acquire information on the moment when the outer wall of the cell is pierced using the measured reaction force information, and automatically inject a predetermined substance into the cell according to the information using the injector 501. Can be injected by In addition, the controller 601 may remove the injector 501 by driving the driver 503 after injecting a predetermined substance into the cell (S805).

The controller may determine whether an injection material is injected into all cells (or a predetermined number of cells) stored in the first cell storage unit 201 of the fluid system 200. If the injection material is injected into all cells, the cell injection method is terminated (S806).

If a predetermined substance is not injected into all cells (or a predetermined number of cells), the process returns to step S802 and repeats steps S802 to S806. Through this process, the cell injection method can be automatically performed.

The invention can be embodied in other specific forms without departing from the spirit and essential features of the invention. Accordingly, the above detailed description should not be construed as limiting in all aspects and should be considered as illustrative. The scope of the invention should be determined by reasonable interpretation of the appended claims, and all changes within the equivalent scope of the invention are included in the scope of the invention. It is also possible to form embodiments by combining claims that do not have an explicit citation in the claims or to include them as new claims by post-application correction.

1 is a view showing a block diagram (a) and a perspective view (b) of a cell injector according to an embodiment of the present invention.

2 is a view showing a fluid system 200 included in the cell injection device 100, according to an embodiment of the present invention.

3 is a diagram illustrating a counter 300 capable of acquiring information about the movement and position of the cell 205, according to an exemplary embodiment of the present invention.

4 is a view showing the principle of operation of the forceps device 400 and the forceps used to hold cells of various sizes moving in the fluid system 200 in a predetermined position, according to an embodiment of the present invention. .

5 is another view showing the principle of operation of the forceps device 400 and the forceps used to hold cells of various sizes moving in the fluid system 200 in a predetermined position, in accordance with one preferred embodiment of the present invention. to be.

6 is a diagram illustrating an example of an injection system 500 and an operation process for injecting a predetermined substance into fixed cells, according to an exemplary embodiment of the present invention.

7 is a view showing the relationship between the reaction force and the distance that can be used in a preferred embodiment of the present invention.

8 is a view showing a process of performing a cell injection method according to an embodiment of the present invention.

Claims (18)

In the cell injection device for injecting a predetermined substance into the cell using the reaction force of the cell, A fluid system for moving said cells; A counter provided at one side of the fluid system to obtain information regarding the movement and position of the cell; A forceps device provided on one side of the fluid system to fix the cells at a predetermined position; An infusion system comprising an injector used to inject a substance into the immobilized cells; And And a force measuring system provided on one side of the injection system to measure reaction force generated when the injector is inserted into the cell. The method of claim 1, A power input unit for supplying power to the cell injection device; And A control unit for recognizing the position of the cell through the counter, driving the forceps device according to the information about the position of the cell, and controlling whether to inject the predetermined substance into the cell using the force measuring system; Further comprising, a cell injection device. The method of claim 1, The fluid system, A first cell reservoir configured to store the cell before injecting the predetermined substance into the cell; A cell tunnel containing a fluid for moving said cells; An injector inlet provided at one side of the cell tunnel and into which the injector is inserted; And And a second cell reservoir in which the cells are stored after the predetermined substance is injected. The method of claim 1, The counter, A transmitter for transmitting a signal for recognizing the movement and location of the cell; And Cell injection device comprising a receiver for receiving a signal transmitted from the transmitter. The method of claim 4, wherein The transmitter is a cell injection device, characterized in that for generating a laser signal or an electromagnetic signal. The method of claim 1, The injection system, An injector for injecting the predetermined substance into cells fixed in the fluid system through an injector inlet provided at one side of the fluid system; An injection material storage unit provided at one side of the injector to store the predetermined material to be injected into the cell; And And a drive unit for driving the injector to inject the predetermined substance into the cell. In the cell injection device for injecting a predetermined substance into the cell using the reaction force of the cell, A fluid system for moving said cells; A counter provided at one side of the fluid system to obtain information regarding the movement and position of the cell; A forceps device which is provided on one side of the fluid system and includes a piezoelectric material that is deformed in a direction in which the predetermined force is applied when the predetermined force is applied to fix the cell at a predetermined position; An infusion system comprising an injector used to inject a substance into the immobilized cells; And And a force measuring system provided on one side of the injection system to measure reaction force generated when the injector is inserted into the cell. The method of claim 7, wherein The fluid system, A first cell reservoir configured to store the cell before injecting the predetermined substance into the cell; A cell tunnel containing a fluid for moving said cells; An injector inlet provided at one side of the cell tunnel and into which the injector is inserted; And And a second cell reservoir in which the cells are stored after the predetermined substance is injected. The method of claim 8, The counter, A transmitter for transmitting a signal for recognizing the flow and location of the cell; And Cell injection device comprising a receiver for receiving a signal transmitted from the transmitter. The method of claim 9, The injection system, An injector for injecting the predetermined material into cells fixed in the fluid system through a injector inlet; An injection material storage unit provided at one side of the injector to store the predetermined material to be injected into the cell; And And a drive unit for driving the injector to inject the predetermined substance into the cell. In the cell injection device for injecting a predetermined substance into the cell using the reaction force of the cell, A fluid system for moving said cells; A counter provided at one side of the fluid system to obtain information regarding the movement and position of the cell; A forceps device provided at one side of the fluid system and fixing the cell by using dielectric electrophoresis generated when an alternating voltage is applied; An infusion system comprising an injector used to inject a substance into the immobilized cells; And And a force measuring system provided on one side of the injection system to measure reaction force generated when the injector is inserted into the cell. In the cell injection method for injecting a predetermined substance into the cell using the reaction force of the cell, When the cells move through the fluid system, obtaining position information of the cells using a counter; Fixing the cell using a forceps device based on the location information; Inserting an injector included in an injection system into the fixed cells; Measuring information about reaction force generated when the injector is inserted into the cell using a force measuring system; And Injecting the predetermined substance into the cell through the injector based on the information on the reaction force. The method of claim 12, The location information, Cell injection method comprising the information on the movement of the cell and the specific location of the cell. The method of claim 12, Before acquiring the location information, And applying pressure to the fluid system such that the cells move through the fluid system. The method of claim 13, After the step of injecting the predetermined material, And removing the injector using a driver included in the injection system. The method of claim 12, The fluid system, A first cell reservoir configured to store the cell before injecting the predetermined substance into the cell; A cell tunnel containing a fluid for moving said cells; An injector inlet provided at one side of the cell tunnel and into which the injector is inserted; And And a second cell reservoir in which the cells are stored after the predetermined substance is injected. In the cell injection method for injecting a predetermined substance into the cell using the reaction force of the cell, When the cells move through the fluid system, obtaining position information of the cells using a counter; Fixing the cell according to the positional information by using a forceps device including a piezoelectric material that fixes the shape in a direction in which the predetermined force is applied and fixes the cell at a predetermined position when a predetermined force is applied. ; Inserting an injector included in an injection system into the fixed cells; Measuring information about reaction force generated when the injector is inserted into the cell using a force measuring system; And Injecting the predetermined substance into the cell through the injector based on the information on the reaction force. In the cell injection method for injecting a predetermined substance into the cell using the reaction force of the cell, When the cells move through the fluid system, obtaining position information of the cells using a counter; Fixing the cell according to the position information by using a forceps device provided at one side of the fluid system and fixing the cell by using dielectric electrophoresis generated when an alternating voltage is applied; Inserting an injector included in an injection system into the fixed cells; Measuring information about reaction force generated when the injector is inserted into the cell using a force measuring system; And Injecting the predetermined substance into the cell through the injector based on the information on the reaction force.

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