KR20060038975A - Method for preparing fermented alcoholic drink using purple sweetpotato - Google Patents
Method for preparing fermented alcoholic drink using purple sweetpotato Download PDFInfo
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Abstract
본 발명은 자색고구마를 이용한 기능성 발효주 제조방법에 관한 것으로, 보다 상세하게는 수용성 안토시아닌 색소를 다량 함유하고 있는 자색고구마를 원료로 하여, 전분질 원료를 사용하면서도 과실주형태로 발효시킴으로써 자색고구마의 색소가 지속적으로 유지할 수 있도록 하고, 우수한 향미와 색택을 갖는 새로운 형태의 발효주를 제조하는 방법에 관한 것이다. The present invention relates to a method for preparing functional fermented liquor using purple sweet potato, and more particularly, using purple sweet potato containing a large amount of water-soluble anthocyanin pigment as a raw material, and fermenting it in fruit wine form using starch raw material to sustain the pigment of purple sweet potato. The present invention relates to a method for producing a new type of fermented liquor having a good flavor and color taste.
본 발명에 따른 자색고구마를 이용한 기능성 발효주 제조방법은 자색고구마의 색소를 안정적으로 유지할 수 있도록 pH를 조절하여 색소파괴에 의하여 품질 저하를 방지하면서 우수한 향미와 색택을 갖는 새로운 형태의 발효주를 제공하는 효과가 있다. Functional fermented liquor manufacturing method using a purple sweet potato according to the present invention is effective to provide a new type of fermented liquor having excellent flavor and color while controlling the pH to maintain the pigment of the purple sweet potato to prevent quality degradation by pigmentation There is.
자색고구마, 안토시아닌, 발효주 Purple sweet potato, anthocyanin, fermented wine
Description
도 1은 본 발명에 따른 자색고구마를 이용한 기능성 발효주의 제조공정도를 도시한 것이다.Figure 1 shows a manufacturing process of the functional fermented wine using a purple sweet potato according to the present invention.
발명의 분야Field of invention
본 발명은 자색고구마를 이용한 기능성 발효주 제조방법에 관한 것으로, 보다 상세하게는 수용성 안토시아닌 색소를 다량 함유하고 있는 자색고구마를 원료로 하여, 전분질 원료를 사용하면서도 과실주형태로 발효시킴으로써 자색고구마의 색소가 지속적으로 유지할 수 있도록 하고, 우수한 향미와 색택을 갖는 새로운 형태의 발효주를 제조하는 방법에 관한 것이다. The present invention relates to a method for preparing functional fermented liquor using purple sweet potato, and more particularly, using purple sweet potato containing a large amount of water-soluble anthocyanin pigment as a raw material, and fermenting it in fruit wine form using starch raw material to sustain the pigment of purple sweet potato. The present invention relates to a method for producing a new type of fermented liquor having a good flavor and color taste.
발명의 배경Background of the Invention
자색고구마는 전분과 단백질 이외에도 비타민과 무기질 및 식이 섬유 등을 풍부하게 함유하고 있고 특히 생리 기능성 물질의 하나로 알려진 수용성 안토시아닌 색소를 다량 함유 제품의 원료로서 활용이 크게 기대되고 있으나, 자색고구마 자체가 식품으로서의 기호성이 떨어지는 점과 전분 분해효소에 의한 당화가 잘 되지 못하는 문제점 등이 있어 다양한 가공식품 개발이 어렵다. In addition to starch and protein, purple sweet potato is rich in vitamins, minerals, and dietary fiber. Especially, it is expected to use water-soluble anthocyanin pigment as a raw material for a large amount of products. It is difficult to develop a variety of processed foods because of poor taste and poor glycosylation by starch degrading enzymes.
발효주 제조에 있어서 종래의 통상적인 제조방법은 곡류나 전분이 함유된 물료를 국과 물료를 원료로 하여 발효시키거나, 과실을 제경, 파쇄·압착하여 과즙을 제조하고 과즙당분이 부족한 경우 설탕 등으로 목표 전화당분까지 보당하며 과즙 중의 유해한 미생물의 번식과 살균 및 산화방지를 위하여 아황산을 첨가하고 발효시킨 후 압착 여과하여 제성하였다. Conventional methods of manufacturing fermented liquor include fermenting cereals and starch-containing ingredients with soups and ingredients, or preparing fruit juices by crushing, crushing, and compressing the fruits, and sugars when the sugar content is insufficient. It was added to the target invert sugar, and was added by fermenting sulfurous acid for fermentation, sterilization, and oxidation prevention of harmful microorganisms in the fruit juice.
자색고구마를 이용한 발효주 제조방법에 관련된 종래 특허로는 '자색고구마를 이용한 생리기능성 발효주와 그 제조방법'(한국특허출원 등록번호 10-0426562, 2004.03.29) 및 '자색고구마를 이용한 고구마 양주 제조방법'(한국특허출원 출원번호 특1994-0020344, 1994.08.18) 등이 있다. 그러나 상기 특허들은 기호, 색도 및 생리기능성 효과가 높은 자색고구마주를 제공하는데 반해, 자색고구마의 색소가 지속적으로 유지할 수 없다는 문제점을 갖고 있다.Conventional patents related to the method for producing fermented liquor using purple sweet potato include 'physiologically functional fermented liquor using purple sweet potato and its manufacturing method' (Korean Patent Application No. 10-0426562, 2004.03.29) and 'method of producing sweet potato liquor using purple sweet potato (Korean Patent Application No. 1994-0020344, 1994.08.18) and the like. However, the above patents provide a purple sweet potato wine having high taste, chromaticity and physiological functional effects, but have a problem in that the pigment of the purple sweet potato cannot be maintained continuously.
이에, 본 발명자들은 종래 기술의 단점을 해결하기 위하여 예의 노력한 결과, 사과산으로 보산하여 pH를 조절함으로써 안토시아닌 색소가 안정적으로 유지되 고, 색소 파괴에 의한 품질 저하를 방지하는 것을 확인함으로써, 본 발명을 완성하게 되었다.Accordingly, the present inventors have made diligent efforts to solve the shortcomings of the prior art, by confirming that the anthocyanin pigment is maintained stably by preventing pH by adjusting acid with malic acid, thereby preventing the degradation of quality due to pigment breakdown. It was completed.
본 발명의 주된 목적은 자색고구마의 색소를 안정적으로 유지하여 품질 저하를 방지할 수 있는 자색고구마를 이용한 기능성 발효주 제조방법을 제공하는데 있다.It is a main object of the present invention to provide a method for producing functional fermented liquor using purple sweet potato which can stably maintain the pigment of the purple sweet potato to prevent the quality deterioration.
상기 목적을 달성하기 위하여, 본 발명은 (a) 자색고구마 100중량부에 대하여 물 130중량부를 가하여 증자하는 단계; (b) 상기 증자된 자색고구마의 당도가 24∼26Brix가 되도록 설탕으로 보당하는 단계; (c) 상기 (a)단계로부터 제조된 증자 자색고구마의 pH가 3.8∼4.0이 되도록 사과산으로 보산하는 단계; (d) 상기 증자된 자색고구마에 β-아밀라제 및 글루코아밀라제를 첨가하여 전분을 당화하는 단계; (e) 상기 (d) 단계에서 수득된 당화액에 효모를 첨가하여 1단 담금으로 20∼25℃에서 5∼7일간 발효시켜 술덧을 제조하는 단계; 및 (f) 상기 제조된 술덧을 압착 여과하여 술지게미를 제거하고, 2차 여과하여 발효주를 제조하는 단계를 포함하는 자색고구마를 이용한 기능성 발효주 제조방법을 제공한다.In order to achieve the above object, the present invention comprises the steps of (a) adding a weight of 130 parts by weight of water based on 100 parts by weight of purple sweet potato; (b) adding sugar to the sweetened purple sweet potato so that the sugar content is 24 to 26 Brix; (C) the step of acidic acid so that the pH of the purple purple sweet potato prepared from step (a) is 3.8 to 4.0; (d) glycosylating starch by adding β-amylase and glucoamylase to the cooked purple sweet potato; (e) adding yeast to the saccharified solution obtained in step (d) to ferment for 5-7 days at 20-25 ° C. in a single stage immersion to prepare drunk; And (f) provides a method for producing a functional fermented liquor using a purple sweet potato comprising the step of eliminating the sake lees by compression filtration of the prepared rice bran, secondary filtration.
본 발명에 있어서, 상기 (d) 단계에서 증자 자색고구마 100중량부에 대하여 β-아밀라제는 0.1~0.5중량부 및 글루코아밀라제는 0.05~0.5중량부를 첨가하는 것을 특징으로 할 수 있다.In the present invention, the β-amylase may be added in an amount of 0.1 to 0.5 parts by weight and glucoamylase in an amount of 0.05 to 0.5 parts by weight based on 100 parts by weight of purple sweet potato in step (d).
본 발명에 있어서, 상기 (e) 단계에서 효모는 탁·약주효모, 맥주효모 및 과 실주효모로 구성된 그룹에서 선택되는 어느 하나를 이용하고, 상기 증자 자색고구마 100 중량부에 대하여 효모 0.05~0.5중량부를 첨가하는 것을 특징으로 할 수 있다.In the present invention, the yeast in the step (e) using any one selected from the group consisting of turbid, medicinal yeast, brewer's yeast and fruit real yeast, 0.05 to 0.5 weight of yeast per 100 parts by weight of the purple purple sweet potato It may be characterized by adding a part.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
우선, 원료인 자색고구마를 세척하고, 자색고구마 100중량부에 대하여 물 130중량부를 가하여 40분간 증자한 다음, 상기 증자된 자색고구마의 당도가 24∼26Brix가 되도록 설탕으로 보당하고, 상기 증자된 자색고구마의 pH가 3.8∼4.0이 되도록 사과산으로 보산한다. 다음으로, 상기 증자 자색고구마 100중량부에 대하여 β-아밀라제는 0.1~0.5중량부 및 글루코아밀라제 0.05~0.5중량부를 첨가하여 자색고구마에 함유된 전분을 당화시키킨다. 또한, 탁·약주효모, 맥주효모, 과실주효모 각각을 상기 증자 자색고구마 100 중량부에 대하여 효모 0.05~0.5중량부를 첨가하여 1단 담금으로 20∼25℃에서 5~7일간 발효시켜 술덧을 제조한다. 상기 제조된 술덧을 압착 여과하여 술지게미를 제거하고, 호기성 초산균, 산막효모와 같은 유해 미생물에 오염되지 않도록 아황산을 보충한 후 앙금분리를 하여 저장 숙성시킨 다음, 2차 여과하여 발효주를 제조한다. First, the purple sweet potato is washed as a raw material, and 130 parts by weight of water is added to 100 parts by weight of the purple sweet potato, followed by 40 minutes of increase. Then, the sweetened purple sweet potato is added with sugar so that the sugar content of 24 to 26 Brix is obtained. The pH of the sweet potato is 3.8-4.0. Next, β-amylase is added 0.1-0.5 parts by weight and 0.05-0.5 parts by weight of glucoamylase to 100 parts by weight of the purple purple sweet potato to saccharify the starch contained in the purple sweet potato. In addition, each of turk, medicinal yeast, brewer's yeast and fruit liquor yeast is added to 0.05 to 0.5 parts by weight of yeast with respect to 100 parts by weight of the cooked purple sweet potato, and fermented at 20 to 25 ° C. for 5 to 7 days in a single immersion to prepare drunk. . Pressurized filtration of the prepared sake to remove the sake lees, supplemented with sulfurous acid so as not to be contaminated with harmful microorganisms such as aerobic acetic acid bacteria, yeast film, sedimentation and storage aging, and then secondary filtration to prepare a fermented liquor.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않을 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and the scope of the present invention will not be construed as being limited by these examples.
실시예 1: 자색고구마주의 색소 안정성 시험Example 1 Pigment Stability Test of Purple Sweet Potato Wine
안토시아닌 색소를 함유하는 제품은 가공이나 장기 저장시 변색이나 퇴색 또는 색소의 파괴에 의하여 품질저하의 주요인이 되므로, 추출방법, 광학조건, pH안정성 시험을 하였다. Products containing anthocyanin pigments were the main cause of quality deterioration due to discoloration, fading, or destruction of pigments during processing or long-term storage.
(1) 추출방법(1) Extraction Method
상온에서 알코올 45%, 알코올 15%, 증류수로 각각 추출하였으며, 추출속도는 알코올 45%, 알코올 15%, 증류수 순이었으며, 알코올로 추출하였을 경우 갈색으로 변색되었으나, 증류수로 추출한 경우 본래의 붉은색이 유지되었다.Extracted with alcohol 45%, alcohol 15%, distilled water at room temperature, and the extraction speed was 45% alcohol, 15% alcohol, distilled water in order, and brown color when extracted with alcohol, but the original red color when extracted with distilled water Maintained.
(2) (2) pHpH 안정성시험Stability test
알코올 45%로 추출한 시험구를 사과산으로 pH6, 5, 4, 3.5, 2.5 및 2로 조정한 후 실내에서 3개월간 방치하였다. The test strips extracted with 45% alcohol were adjusted to pH 6, 5, 4, 3.5, 2.5 and 2 with malic acid and left for 3 months indoors.
3개월 경과 후, pH6과 pH5에서는 흑갈색으로 변색이 되었으나 pH4과 pH3.5에서는 초기와 같은 색상을 유지하였고, pH2.5와 pH2에서는 색상이 엷어졌으며, pH4와 pH3.5에서 가장 안정됨을 알 수 있었다.After 3 months, the color changed to blackish brown at pH 6 and pH 5, but the same color was maintained at pH 4 and pH 3.5, the color faded at pH 2.5 and pH 2, and the most stable at pH 4 and pH 3.5. there was.
실시예2: 자색고구마 발효주의 제조Example 2 Preparation of Purple Sweet Potato Fermented Wine
우선, 자색고구마 1㎏을 세척하고 물 1.3ℓ를 첨가하여 40분간 증자한 다음, 당도를 측정하니 상기 증자된 자색고구마는 7Brix였다. 상기 증자된 자색고구마의 Brix가 약 24Brix에 이르도록 설탕 405g을 첨가하고, 안토시아닌 색소가 가장 안정되는 pH3.8∼4.0이 되도록 사과산을 이용하여 보산하였다. First, 1 kg of purple sweet potatoes were washed, 1.3 liters of water were added thereto, and the mixture was cooked for 40 minutes. The sweetness of the purple sweet potatoes was 7 Brix. 405 g of sugar was added so that the Brix of the cooked purple sweet potato reached about 24 Brix, and was supplemented using malic acid so that the anthocyanin pigment was pH 3.8 to 4.0, which is the most stable.
다음으로, 상기 증자된 자색고구마에 당화효소인 β-아밀라제 2g과 글루코아밀라제 1.1g을 첨가하여 전분을 당화하고, 상기에서 수득된 당화액에 탁·약주효모, 맥주효모 및 과실주효모를 각각 1g을 첨가하여 25℃에서 일주일간 발효시켰다. 발효가 완료된 후, 압착 여과하여 술지게미를 제거하고 호기성 초산균, 산막효모와 같은 유해 미생물에 오염되지 않도록 아황산을 보충한 후 앙금분리를 하여 저장 숙성시킨 다음, 2차 여과하여 알코올분 12.2%∼14.1%의 발효주를 1,900㎖를 제조하였다.Next, 2 g of beta-amylase and 1.1 g of glucoamylase, which are glycosylated enzymes, were added to the cooked purple sweet potato, and starch was glycosylated, and 1 g of turbid and medicinal yeast, beer yeast, and fruit main yeast were added to the obtained saccharified solution. It was added and fermented at 25 ° C. for one week. After the fermentation is completed, by pressing filtration to remove the sake lees, supplemented with sulfurous acid so as not to be contaminated with harmful microorganisms such as aerobic acetic acid bacteria, mountain membrane yeast, sedimentation and storage aging, and then secondary filtration to 12.2% to 14.1% alcohol content 1,900 ml of fermented wine was prepared.
(1) 탁·약주발효용 효모 첨가(1) Addition of yeast for fermentation
증자된 자색고구마를 보당 및 보산하고, 당화효소제를 첨가한 다음, 탁·약주효모 1g을 투입하여 7일간 발효시킨 후 분석기기를 이용하여 향미성분을 정량 분석하였다. The sweet purple potato was added and supplemented, and the saccharifying enzyme was added. Then, 1 g of turbid and medicinal yeasts were added and fermented for 7 days, followed by quantitative analysis of flavor components using an analyzer.
(2) 맥주발효용 효모 첨가(2) Yeast for beer fermentation
증자된 자색고구마를 보당 및 보산하고, 당화효소제를 첨가한 다음, 맥주효모 1g을 투입하여 7일간 발효시킨 후 분석기기를 이용하여 향미성분을 정량 분석하였다. The sweetened purple sweet potato was added and supplemented, and the saccharifying enzyme was added, and 1 g of beer yeast was added thereto, followed by fermentation for 7 days, followed by quantitative analysis of flavor components using an analyzer.
(3) 과실주발효용 효모 첨가(3) adding yeast for fruit wine fermentation
증자된 자색고구마를 보당 및 보산하고, 당화효소제를 첨가한 다음, 과실주 발효용 효모 1g을 투입하여 7일간 발효시킨 후 분석기기를 이용하여 향미성분을 정량 분석하였다. The sweet purple potato was added and supplemented, and the glycosylating agent was added. Then, 1 g of fruit liquor fermented yeast was added and fermented for 7 days, followed by quantitative analysis of flavor components using an analyzer.
하기에 실시예 2에서 제조하는 자색고구마를 증자하여 설탕으로 보당하고, 사과산으로 보산한 후 당화효소제와 효모를 첨가하여 발효시키는 담금배합표는 [표 1]에, 일반 성분분석결과는 [표 2]에, GC에 의한 향미성분 분석결과는 [표 3]에 나타내었다. Below, the purple sweet potato prepared in Example 2 is added with sugar, supplemented with malic acid and fermented with a glycosylating agent and yeast, followed by fermentation formulation in [Table 1], and the results of the general component analysis are shown in [Table 2]. The results of flavor component analysis by GC are shown in [Table 3].
상기 [표 2]의 일반 성분분석은 국세청기술연구소 주류분석규정에 의하였다. The general component analysis of [Table 2] was based on the Liquor Analysis Regulations of the National Institute of Technology.
또한, 제품의 향미성분 동향을 파악하기 위하여 아세트알데하이드, 에틸아세테이트, 메탄올, 퓨젤유 등의 성분에 대하여 가스크로마토그래피를 이용하여 외부표준법으로 분석하였으며, 각 성분함량은 하기 [표 3]과 같다.In addition, in order to grasp the trend of the flavor component of the product, the components such as acetaldehyde, ethyl acetate, methanol, fusel oil, etc. were analyzed by gas chromatography using an external standard method, and the contents of each component are shown in Table 3 below.
상기 [표 3]의 GC에 의한 향미성분 분석은 국세청기술연구소 주류분석규정에 의하였으며, 본 발명에서 가스크로마토그래피 분석조건은 다음과 같다: 분석기종은 HP6890 Series system; Detector는 FID; Column은 PEGHT; Injector 온도는 170℃; Detector 온도는 220℃; 및 Injection Volumn은 10㎕.The flavor component analysis by GC of Table 3 was based on the Liquor Analysis Regulations of the National Institute of Technology Research, and the gas chromatography analysis conditions in the present invention are as follows: Analyzer species is HP6890 Series system; Detector is FID; Column is PEGHT; Injector temperature is 170 ° C; Detector temperature is 220 ℃; And Injection Volumn is 10 μl.
상기 [표 3]에 나타낸 바와 같이, 제조된 발효주의 색택은 모두 진한 선홍색으로 증자 초기의 색상을 유지하였으며, 향기와 맛은 각각 효모의 특색을 잘 나타내어 탁·약주효모를 사용한 시험구는 고구마의 향이 가장 풍부하고 가볍고 상큼한 맛을 나타내었고, 맥주용 효모를 사용한 시험구는 향과 맛이 엷고 부드러운 특징으로 나타났으며, 과실주용 효모를 사용한 시험구는 신맛과 떫은맛이 어울러져 과실주의 향과 맛을 어느 정도 느낄 수 있는 것으로 나타났다. 또한 고구마를 원료로 한 술의 특성향인 시트로네롤, 게라니올, 리나놀 등의 테르펜계 알코올과 부패한 고구마에서 나는 페닐초산에틸 향이 생성되는 것으로 나타났다.As shown in [Table 3], all of the fermented wines produced were dark crimson and retained the color of the early stage of cooking, and the fragrance and taste of the yeast showed the characteristics of yeast, respectively. The most abundant, light and refreshing taste was shown, and the test using the yeast for beer was characterized by a light and soft taste. The test using the yeast for fruit was sour and astringent to feel the aroma and taste of the fruit wine. It turns out that you can. It was also found that ethyl phenyl acetate was produced from terpene alcohols such as citronolol, geraniol, and linanol, and rotted sweet potatoes.
이상으로, 본 발명의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다. As described above, specific parts of the present invention have been described in detail, and it is apparent to those skilled in the art that such specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.
이상에서 상세히 설명한 바와 같이, 본 발명은 자색고구마의 색소를 안정적으로 유지하여 품질 저하를 방지할 수 있는 자색고구마를 이용한 기능성 발효주 제조방법을 제공하는 효과가 있다. 본 발명에 따른 자색고구마를 이용한 기능성 발효주 제조방법은 생리 기능성 물질의 하나로 알려져 있는 수용성 안토시아닌 색소가 다량 함유되어 있는 자색고구마를 이용하여 사과산으로 보산하여 pH를 조절함으로써 자색고구마의 안토시아닌 색소가 안정적으로 유지되고, 색소 파괴에 의한 품질 저하를 방지하는 효과가 있다. 통상적으로 젖산이나 구연산 등으로 보산을 하였으나, 사과산으로 보산하여 pH를 조절함으로써 관능결과 가장 우수하게 나타났다. 뿐 만 아니라, 전분질 원료를 사용하면서도 과실주형태로 발효시킴으로써 자색고구마의 색소가 지속적으로 유지할 수 있도록 하고, 우수한 향미와 색택을 갖는 새로운 형태의 발효주를 제공함으로써 발효주 시장의 매출 증대 효과로 주류산업에 크게 이바지할 수 있으며, 자색고구마 재배 농가의 소득 증대에도 기여할 수 있다. As described in detail above, the present invention has the effect of providing a functional fermented liquor manufacturing method using a purple sweet potato that can stably maintain the pigment of the purple sweet potato to prevent the degradation of quality. Functional fermented liquor manufacturing method using a purple sweet potato according to the present invention by using a purple sweet potato containing a large amount of water-soluble anthocyanin pigment known as one of the physiological functional substances is added to malic acid to adjust the pH to maintain a stable anthocyanin pigment of the purple sweet potato It is effective in preventing the deterioration of quality due to pigment breakage. Normally, lactic acid or citric acid was supplemented with acid, but with malic acid, the pH was adjusted to show the best sensory results. In addition, by using starch raw materials and fermenting in the form of fruit wine, the color of the purple sweet potato can be maintained continuously, and by providing a new type of fermented wine with excellent flavor and color, it greatly increases the liquor industry's sales. It can contribute and contribute to the income increase of purple sweet potato farmers.
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