KR20050037107A - Cosmetic composition against aging of the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles, wherein the cosmetic composition for improving skin wrinkles comprising at least one extract selected from the group consisting of sarcoma extract and hyacinth extract as an active ingredient It relates to a composition.

The cosmetic composition for improving skin wrinkles of the present invention has a strong collagen synthesis promoting effect, and when applied to the skin, the wrinkle improving effect is very excellent, and no side effects occur.

Description

Cosmetic composition for improving skin wrinkles {Cosmetic Composition against Aging of the Skin}

[Industrial use]

The present invention relates to a cosmetic composition for improving skin wrinkles, and more particularly, to a skin composition for improving skin wrinkles by promoting collagen synthesis of fibroblasts of the skin to improve skin elasticity and improving skin wrinkles. will be.

[Private Technology]

Collagen, a major component of the extracellular matrix, is present in extracellular epilepsy as a major substrate protein produced in skin fibroblasts. It is also an important protein, accounting for about 30% of the total weight of biological proteins, and has a solid triple helix structure. Collagen forms most of the organic material of the skin, tendons, bones and teeth, especially in bones and skin (dermis). Most other sieve structures exist as fibrous inclusions.

Collagen is a relatively weak immunogen, partly due to the shielding of latent antigenic determinants by the helix structure of collagen, which also makes collagen resistant to proteolysis. The main functions of collagen are known as mechanical firmness of skin, resistance of connective tissue and binding of tissue, support of cell adhesion, induction of cell division and differentiation (in organic growth or wound healing) (Van der Rest et al. 1990). This collagen is reduced by age and photoaging by UV irradiation, which is known to be closely associated with wrinkle formation of the skin (Arthur K. Balin et al., "Aging and the skin", 1989). In recent years, extensive research on aging of the skin has been developed to reveal the important function of collagen in the skin.

Conventionally, products using collagen have been released in order to take advantage of collagen's skin moisturizing effect.However, these cosmetics apply collagen to the surface of the skin. It could not be said to be an essential skin function improvement.

In order to solve this problem, interest in collagen synthesis promoting material has been increased, and conventionally known collagen synthesis promoting materials include vitamin C and its derivatives, retinoic acid, and carcinogenic growth factor (TGF). Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974), protein from animal placenta (JP8-231370), betulinic acid (JP8-208424), chlorella extract (JP9-40523, JP10-36283, fibroblast proliferation promoting effect).

However, these substances have limited use or insignificant effects due to safety problems such as irritation and redness during skin application, and thus, a substantial improvement in skin function cannot be expected. Therefore, there is an urgent need for the development of a new skin wrinkle improvement cosmetic composition that is safer and more effective in vivo than the existing skin wrinkle improvement cosmetic composition.

The present invention is to solve the conventional problems as described above, an object of the present invention to promote the synthesis of collagen to improve the elasticity of the skin, to provide a cosmetic composition for improving skin wrinkles excellent skin wrinkles will be.

In order to achieve the above object, the present invention provides a collagen synthesis promoter composition comprising as an active ingredient an extract selected from the group consisting of sarcoma extract and jaehwa (紫 초 花) extract as an active ingredient.

The present invention also provides a cosmetic composition for improving skin wrinkles comprising at least one extract selected from the group consisting of a breeding extract and a soybean extract as an active ingredient.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The inventors of the present invention, while developing a substance having an excellent collagen synthesis promoting effect as an active ingredient of the cosmetic composition for improving skin wrinkles, it was found that the extracts for breeding and the soybean extract have a very strong collagen synthesis promoting effect, which is very excellent in improving skin wrinkles. The present invention was completed.

Breeding is a fleshy stem of the breeding plants of the Orobanchaceae, Cistanche salsa, Cistanche deserticola and Cistanche ambigua . Sarcoma is used for the treatment of male genitalia and infertility in women. Substances such as decanoyl-beta-D-glucopyranoside have been identified. .

Jahwahwa (紫 梢 花) is a sponge of the Spongilladae that lives in rivers or lakes. It treats lobster, urine whitening, incontinence, and scrotum eczema. Spongin, spongenin, and the like have been reported. However, there have been no patents related to promoting collagen biosynthesis and improving wrinkles of soybean extract.

The collagen biosynthesis promoting extract of the present invention may be selected from one or more of the group consisting of breeding and self-priming to extract a solvent extract one or more times with water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, respectively, In addition, the solvent extract is distilled under reduced pressure, followed by freeze drying or spray drying to obtain a dry extract.

Examples of extraction methods, such as breeding, commercialization and mixtures thereof commercially available as a herbal medicine, finely pulverized, 5 to 20 volumes of water, anhydrous or hydrous lower alcohol, ethyl having 1 to 4 carbon atoms based on the dry weight of the ground powder Extraction is carried out with acetate or acetone by heating at 50-100 ° C. for 1-5 hours in an extractor equipped with a reflux condenser. The extract was filtered with a filter cloth, and the residue was extracted one more time in the same way and filtered to obtain a solvent extract.

In addition, the filtered solvent extract extracts are combined and concentrated under reduced pressure, followed by freeze drying or spray drying to obtain a dry extract.

Breeding, self-cultivation and mixed extract thereof prepared in this way shows a very powerful collagen biosynthesis promoting effect on human-derived fibroblasts. In addition, when an external skin ointment or cosmetics are prepared as a dry sarcoma extract or a soybean extract, and applied to human skin, wrinkle improvement is very excellent.

By producing a cosmetic composition for improving skin wrinkles comprising the above breeding, self-priming and mixed extract thereof as an active ingredient and applied to the human body, it is possible to obtain an excellent skin elasticity and wrinkle improvement effect.

At this time, the cosmetic composition for improving skin wrinkles may include 0.0001% to 10% by weight of the dry weight with respect to the total weight of one or more extracts selected from the group consisting of breeding and self-inflation as a collagen production promoter, preferably 0.0005 weight % To 10% by weight may be included, and more preferably 0.01 to 10% by weight. At a concentration of less than 0.0001% by weight, no obvious effect can be expected, and at a concentration of 10% by weight or more, no apparent effect increases with the increase of the content.

In addition, in the present invention, when used in combination with the breeding and self-priming extract can further enhance the collagen production promoting effect. The weight ratio of the components of the breeding extract and the soybean extract is preferably 3: 1 to 1: 3.

The cosmetic composition for improving skin wrinkles according to the present invention is an external ointment, cream, foam, lotion, pack, soft water, emulsion, foundation, makeup base, essence, soap, liquid cleanser, bathing agent, sunscreen cream, or sun oil It may be prepared in a dosage form.

In addition, the cosmetic composition of the present invention can be appropriately blended with conventional ingredients, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances and the like, which are blended in general skin cosmetics.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

[Examples 1 to 12] Extract Extract of Breeding or Forage

After crushing commercially available breeding or pyrophyllization, 100 g of the pulverized product is placed in 3 liters of water or a solvent having 1 to 4 carbon atoms, anhydrous or hydrous lower alcohol, ethyl acetate or acetone, and a reflux extractor equipped with a cooling capacitor. The mixture was boiled for 3 hours, extracted, filtered through a 100 mesh filter cloth, and the residue was extracted once more in the same manner. The extracts obtained in each of the above steps were combined and filtered using a filter filter of Whatman No. 2 at room temperature to remove insoluble materials, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, followed by freeze drying to obtain a dry extract. . The dry weights of the herbs, solvents and dry extracts obtained in each case are shown in Table 1 below.

TABLE 1

Breeding Sleeping flowers division Solvent Extract dry weight (g) division Solvent Extract dry weight (g) Example 1 80% methanol 5.5 Example 7 80% methanol 3.3 Example 2 Purified water 5.3 Example 8 Purified water 3.3 Example 3 85% ethanol 5.6 Example 9 85% ethanol 4.2 Example 4 70% acetone 4.0 Example 10 70% acetone 3.1 Example 5 Ethyl acetate 4.1 Example 11 Ethyl acetate 2.5 Example 6 Saturated normal butanol 2.0 Example 12 Saturated normal butanol 2.1

Test Example 1 Collagen Biosynthesis Effect of Extracts of Examples 1 to 12

Each extract obtained according to Examples 1 to 12 was added to the culture solution of human-derived fibroblasts to test collagen synthesis promoting effect at the cellular level. Biosynthetic collagen was measured using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).

The concentration of each extract obtained according to Examples 1 to 12 was added to the culture medium of the fibroblasts derived from humans so that the final concentration was 10 ㎍ / ml, and cultured for 1 day, and then the culture solution was taken and each concentration was obtained by PICP EIA Kit. The degree of collagen biosynthesis at was measured at 450 nm using a spectrophotometer. In order to compare the collagen biosynthesis effect, the same measurement was performed for the culture medium and the control group without the extract containing vitamin C (Vitamin C). Collagen biosynthesis was calculated as the relative synthetic performance of the control (no addition) and the results are summarized in Table 2.

[Table 2] Collagen Biosynthesis Effect at the Cell Level of Sarcoma and Spontaneous Extracts

sample ingredient Final concentration (㎍ / ml) % Increase Control No addition - - Example 1 Breeding extract 10 137 Example 2 Breeding extract 10 128 Example 3 Breeding extract 10 143 Example 4 Breeding extract 10 144 Example 5 Breeding extract 10 141 Example 6 Breeding extract 10 133 Example 7 Japonica Extract 10 132 Example 8 Japonica Extract 10 129 Example 9 Japonica Extract 10 138 Example 10 Japonica Extract 10 138 Example 11 Japonica Extract 10 143 Example 12 Japonica Extract 10 130 Comparative Example Vitamin c 52.8 130.4

                                              * Number of replicates = 6

As can be seen from the results of Table 2, the samples obtained according to Examples 1 to 12 have very good collagen biosynthesis promoting effect on human-derived fibroblasts, even though the extracts were not purified at all, and generally have collagen synthesis ability. It can be seen that better collagen synthesis can be obtained at a lower concentration than the known vitamin C.

[Test Example 2]

In the same manner as in Test Example 1, the collagen biosynthesis promoting effect according to the concentration of the extracts of Examples 1 and 7 was investigated with respect to the fibroblasts derived from humans, and the results are shown in Table 3.

[Table 3] Collagen biosynthesis promoting effect at the cellular level according to the concentration of the extract

sample ingredient Final concentration (㎍ / ml) % Increase Control No addition - - Example 1 Breeding extract 5 120 10 137 20 139 50 146 Example 7 Hydration extract 5 122 10 132 20 135 50 143 Comparative Example Vitamin c 52.8 130

                                                             * Repetitions = 6

As can be seen from the results of Table 3, the sarcoma extract and the self-extracting extract have a very strong collagen biosynthesis promoting effect on human-derived fibroblasts. In addition, it can be seen that the collagen biosynthesis promoting effect increases as the content of the breeding extract and the soybean extract increases. Therefore, the sarcoma extract and the self-priming extract may be usefully used as an extract for promoting collagen biosynthesis.

[Test Example 3]

A 6-week-old hairless mouse was used to test the anti-wrinkle effect of the sarcoma extract and the apricot extract against light-induced wrinkles. The extracts of Examples 3 and 9 were dissolved in 5 mg / ml in 1,3-butylene glycol, and the test method was as follows.

Hairless mice were irradiated with 2MED for 3 days and 10 days a week using a solar simulator to form wrinkles, and 5 mg / of a control treated with 1,3-butylene glycol without addition of the sample. The improvement degree was qualitatively determined for the group treated with 6 ml of breeding and self-priming extract of 0.5 ml / cm ² (2.5 mg / cm ² with the extracts of Examples 3 and 9) twice a day for 6 weeks.

Wrinkle improvement was judged by visual inspection and photographing of the sample treatment area first, and the criterion was no improvement (1 point), slight improvement (2 points), compared to the sample treatment group and the control group before the sample treatment. Table 3 shows the three stages of significant improvement (three points).

[Table 4] Wrinkle improvement effect on the mouse

sample No improvement (× 1) Slight improvement (× 2) Significant improvement (× 3) Average score Control 10 horses 0 0 1 point Example 3 (extracts for breeding) 1 horse 5 horses 4 horses 2.3 points Example 9 (Spontaneous Extract) 0 5 horses 5 horses 2.5 points

                                                   Population in each group = 10

In Test Examples 1 to 3 described above, no skin irritation or side effects were observed by the sarcoma extract and the soybean extract of the present invention.

Hereinafter, the preparation examples and comparative examples of the cosmetic composition for improving skin wrinkles, including the sarcoma extract and the jaehwa extract.

EXAMPLE 13, 14 AND COMPARATIVE EXAMPLE 1 Skin external ointment for skin wrinkle improvement

Formulations of external skin ointment for skin wrinkles including sarcoma extract and self-improvement Formulations of external skin ointment for skin wrinkles including extract for extract were prepared in Examples 13 and 14, respectively. Except not including the extract, Comparative Example 1 to prepare a skin external ointment in the same manner as in Examples 13 and 14. Detailed compositions are shown in Table 5 below.

TABLE 5

Composition water Example 13 (% by weight) Example 14 (% by weight) Comparative Example 1 (% by weight) extract Breeding extract 10 - - Hydration extract - 10 - Diethyl sebacate 8 8 8 Prepayment 5 5 5 Polyoxyethylene Oleyl Ether Phosphate 6 6 6 Sodium benzoate 0.01 0.01 0.01 Waselin to 100 to 100 to 100

[Examples 15, 16 and Comparative Example 2] Cosmetic Cream for Skin Wrinkle Improvement

Formulations of the cosmetic cream for skin wrinkle improvement comprising the extract for breeding and formulations of the cosmetic cream for skin wrinkle improvement including the soybean extract were prepared in Examples 15 and 16, respectively, the extract of sarcoma and extract of A cosmetic cream was prepared in the same manner as in Examples 15 and 16 except that it was not included as Comparative Example 2. Detailed compositions are shown in Table 6 below.

TABLE 6

Composition water Example 15 (% by weight) Example 16 (% by weight) Comparative Example 2 (wt%) extract Breeding extract 1.0 - - Hydration extract - 1.0 - Stearic acid 15.0 15.0 15.0 Cetanol 1.0 1.0 1.0 Potassium hydroxide 0.7 0.7 0.7 glycerin 5.0 5.0 5.0 Propylene glycol 3.0 3.0 3.0 antiseptic 0.05 0.05 0.05 incense 0.05 0.05 0.05 Purified water to 100 to 100 to 100

[Examples 17, 18 and Comparative Example 3] supple cosmetics for improving skin wrinkles

Formulations of the skin for improving wrinkle wrinkles including sarcoma extract and formulation of skin softening for the improvement of skin wrinkles comprising a soybean extract were prepared in Examples 17 and 18, respectively, the extracts of sarcoma and extract of Except not including the same as in Example 17 and 18 to prepare a flexible longevity was set to Comparative Example 3. The detailed composition is shown in Table 7 below.

TABLE 7

Composition water Example 17 (% by weight) Example 18 (% by weight) Comparative Example 3 (wt%) extract Breeding extract 0.2 - - Hydration extract - 0.2 - ethanol 10.0 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 0.2 glycerin 5.0 5.0 5.0 1,3-butylene glycol 6.0 6.0 6.0 incense 0.05 0.05 0.05 Pigment 0.05 0.05 0.05 Purified water to 100 to 100 to 100

[Examples 19, 20 and Comparative Example 4] Nourishing lotion for improving skin wrinkles

Formulations of skin wrinkle improvement nutrient for longevity including sarcoma extract and formulation of skin wrinkle improvement nutrient for longevity including soybean extract were prepared in Examples 19 and 20, respectively. Comparative Example 4 was prepared in the same manner as in Examples 19 and 20 except that it does not contain. The detailed composition is shown in Table 8 below.

TABLE 8

 Composition water Example 19 (% by weight) Example 20 (% by weight) Comparative Example 4 (wt%) extract Breeding extract 0.1 - - Hydration extract - 0.1 - Waselin 2.0 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 1.2 Methyl paraoxybenzoate 0.1 0.1 0.1 Propylene glycol 5.0 5.0 5.0 ethanol 3.2 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 18.0 Potassium hydroxide 0.1 0.1 0.1 Pigment 0.05 0.05 0.05 incense 0.05 0.05 0.05 Purified water to 100 to 100 to 100

[Examples 21, 22 and Comparative Example 5] Cosmetic Pack for Skin Wrinkle Improvement

Formulations of the skin wrinkle improvement cosmetic pack comprising a sarcoma extract and formulations of the skin wrinkle improvement cosmetic pack comprising a soybean extract were prepared in Examples 21 and 22, respectively, wherein the sarcoma extract and A cosmetic pack was prepared in the same manner as in Examples 21 and 22 except that it was not included as Comparative Example 5. Detailed compositions are shown in Table 9 below.

TABLE 9

 Composition water Example 21 (% by weight) Example 22 (% by weight) Comparative Example 5 (wt%) extract Breeding extract 0.5 - - Hydration extract - 0.5 - glycerin 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 Polyvinyl alcohol 15.0 15.0 15.0 ethanol 8.0 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 0.2 Pigment 0.05 0.05 0.05 incense 0.05 0.05 0.05 Purified water to 100 to 100 to 100

[Examples 23, 24 and Comparative Example 6] Cosmetic Essence for Skin Wrinkle Improvement

Formulations of the skin wrinkle improvement cosmetic essence comprising the sarcoma extract and formulations of the skin wrinkle improvement cosmetic essence comprising the saponification extract were prepared in Examples 23 and 24, respectively, in the above examples A cosmetic essence was prepared in the same manner as in Examples 23 and 24 except that it was not included as Comparative Example 6. The detailed composition is shown in Table 10 below.

TABLE 10

 Composition water Example 23 (% by weight) Example 24 (% by weight) Comparative Example 6 (wt%) extract Breeding extract 5 - - Hydration extract - 5 - Propylene glycol 10.0 10.0 10.0 glycerin 10.0 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 5.0 ethanol 5.0 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 0.1 incense 0.05 0.05 0.05 Purified water to 100 to 100 to 100

[Test Example 5] Wrinkle improvement effect test of the cosmetic essence for skin wrinkle improvement of the present invention

The skin wrinkle improvement effects of the essences of Examples 23 and 24 and Comparative Example 6 were tested in healthy 35 to 50 year old women as follows.

Thirty women aged 35 to 50 years old were divided into three groups of 10 persons, and the first group contained the essence of Example 23 including 5% by weight of the sarcoma extract, and the second group contained 5% by weight of the acupuncture extract. The essence of Example 24 was applied to group 3, and the essence of comparative example 6 was applied to the face part once a day for 3 months.

After 3 months, the improvement of skin wrinkles was evaluated by subject's questionnaire and image analysis of skin wrinkles. The subject's questionnaire was judged in three stages of improvement (1 point), slight improvement (2 points), and significant improvement (3 points) in comparison with the pre-use regarding skin wrinkle improvement and elasticity improvement. The results are shown in Table 11. .

[Table 11] Skin wrinkle improvement effect of the cosmetic essence for skin wrinkle improvement of the present invention (person)

sample No improvement (× 1) Slight improvement (× 2) Significant improvement (× 3) Average score Comparative Example 6 8 people 2 people 0 people 1.2 points Example 23 2 people 5 people 3 people 2.1 points Example 24 1 person 6 people 3 people 2.2 points

As can be seen in Table 11, when using the essence of Examples 23 and 24 according to the present invention it can be seen that the skin wrinkle improvement effect is excellent.

As described above, one or more extracts selected from the group consisting of sarcoma and self-inflamation have a very strong collagen biosynthesis promoting effect on fibroblasts derived from humans, and have no side effects, and thus the cosmetic composition containing these extracts improves skin wrinkles. It can be seen that the effect is very excellent.

The cosmetic composition for improving skin wrinkles of the present invention includes at least one extract selected from the group consisting of sarcoma and soybean, which has an effect of promoting collagen synthesis of fibroblasts of the skin, thereby improving skin elasticity and improving skin wrinkles. Is very excellent.

Claims (10)

Collagen synthesis promoter composition comprising as an active ingredient an extract selected from the group consisting of sarcoma extract and jaehwa (紫 梢 花) extract as an active ingredient. The solvent extract according to claim 1, wherein the extract for improving skin wrinkles is selected from the group consisting of breeding and self-priming, and at least one extraction with water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate and acetone, respectively. Collagen synthesis promoter composition, characterized in that. The method of claim 1, wherein the extract for improving skin wrinkles is selected from the group consisting of breeding and self-priming to extract one or more times with water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, respectively, to obtain an extract And a dry extract obtained by distillation under reduced pressure of the extract, followed by freeze drying or spray drying. A cosmetic composition for improving skin wrinkles comprising at least one extract selected from the group consisting of a breeding extract and a soybean extract as an active ingredient. The method according to claim 4, wherein the extract is a solvent extract obtained by extracting at least one of water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, each of at least one selected from the group consisting of breeding and self-inflation Cosmetic composition for. The method of claim 4, wherein the extract is one or more selected from the group consisting of breeding and self-priming to extract one or more times with water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, respectively, to obtain an extract, A cosmetic composition for improving skin wrinkles, which is a dry extract obtained by distillation under reduced pressure and freeze drying or spray drying. The cosmetic composition for improving skin wrinkles according to claim 4, wherein the extract comprises 0.0001% to 10% by weight of the extract as a dry weight based on the total weight of the composition. The cosmetic composition for improving skin wrinkles according to claim 4, wherein the extract comprises 0.0005% by weight to 10% by weight in dry weight, based on the total weight of the composition. The cosmetic composition for improving skin wrinkles according to claim 4, wherein the extract comprises 0.01 wt% to 10 wt% of a dry weight based on the total weight of the composition. The cosmetic composition for improving skin wrinkles according to claim 4, wherein the cosmetic composition for improving skin wrinkles is an external ointment, cream, foam, lotion, pack, soft water, latex, foundation, makeup base, essence, soap, liquid cleanser, bath agent, sunscreen cream, and sun. Cosmetic composition for improving skin wrinkles having a formulation selected from the group consisting of oils.