KR102698518B1 - Feminine cleanser with escellent antibacterial property containing natural extract - Google Patents

Abstract

The present invention relates to a freeze-dried feminine hygiene product containing a natural extract as an effective ingredient. More specifically, the present invention relates to a freeze-drying technique for producing a complex fermented extract using a Lactobacillus spp. strain of a complex extract of mugwort, green tea, purslane, and Bogum cactus stem, and containing the same as an effective ingredient.

Description

{FEMININE CLEANSER WITH ESCELLENT ANTIBACTERIAL PROPERTY CONTAINING NATURAL EXTRACT}

The present invention relates to a freeze-dried feminine wash containing a natural extract as an active ingredient. More specifically, the present invention relates to a freeze-dried feminine wash containing a complex extract prepared by fermenting a complex extract of mugwort ( Artemisia princeps ), green tea ( Camellia Sinensis Leaf), centella asiatica , and opuntia ficus - indica stem using a Lactobacillus spp. strain as an active ingredient.

The female urogenital system includes the uterus, vagina, vulva, and urethra, and is easily exposed to external infections due to its anatomical and physiological or functional characteristics. For example, the vagina is rich in nutrients and is located deep inside the body, so the temperature and humidity are high, making it easy for various germs, including bacteria and fungi that are the main causes of vaginitis, to invade, which increases the possibility of various diseases occurring. Therefore, it is advisable to always keep the vagina clean and take care not to expose it to external bacterial infections.

In addition, the female urogenital system contains lactobacilli that maintain the pH of the urogenital system slightly acidic, which plays a role in preventing the growth of harmful bacteria. However, in addition to physical damage, stress, and hormonal imbalance, diseases related to the female urogenital system can easily occur due to an unclean urogenital environment, bacterial diseases caused by sexual intercourse, or sexually transmitted diseases. In addition, wounds on the mucous membrane that occur when introducing medical devices into the body or during childbirth increase the chance of infection by pathogens and can cause inflammation.

These female urogenital inflammations are one of the most common diseases among patients visiting gynecologists, and they are very diverse, ranging from bacterial to parasitic and viral. These patients are accompanied by itching, severe inflammation, and bad odor. In particular, human papillomavirus infection is closely related to cervical cancer, so careful attention is required. Therefore, it is very important to reduce fatal complications through appropriate prevention, diagnosis, and treatment, and feminine hygiene products are often used to prevent such female diseases and remove unpleasant odors.

Local cleansers used to prevent vaginitis include those containing sulfonated fatty acids or distilled lauryl alcohol, emollients, thickeners, nutrients, foaming agents, organic sterilizers, etc., or those with surfactants and pharmaceuticals with cleansing power, such as povidone-iodine as their main ingredient. However, iodine, the main ingredient of chemical feminine cleansers, may cause hypersensitivity symptoms, itching and burning sensation due to mucosal damage, skin ulcers, contact dermatitis, etc., and it is known that inorganic iodine in the vagina or total iodine level in the blood may increase, causing hypothyroidism.

Since feminine hygiene products are used on sensitive mucous membrane areas, the use of chemically synthesized substances is unsafe. Accordingly, there is an urgent need to develop a new feminine hygiene composition using natural substances that is more effective and safer than previously known feminine hygiene products.

Accordingly, the present invention relates to a freeze-dried feminine cleanser containing a natural extract as an active ingredient and a method for producing the same, and more particularly, to a freeze-dried feminine cleanser containing a complex extract using mugwort ( Artemisia princeps ), green tea ( Camellia Sinensis Leaf), centella asiatica , and Opuntia ficus - indica Stem, a complex fermented extract using Lactobacillus spp., and the same as an active ingredient, and a method for producing the same. The feminine cleanser exhibits excellent antibacterial activity against four types of harmful strains, Pseudomonas aeruginosa , Candida albicans, Escherichia coli , and Bacillus cereus .

10-2015-0148530

The purpose of the present invention is to provide a freeze-dried feminine hygiene product containing a composite extract of mugwort, green tea, purslane, and cactus stem as effective ingredients.

In addition, another object of the present invention is to provide a method for producing a herbal medicine by using a compound extract of mugwort, green tea, centella asiatica and cactus stem, and at least one of Lactobacillus acidophilus , Lactobacillus rhamnosus , Lactobacillus paracasei , Lactobacillus casei , Lactobacillus bulgaricus , Lactobacillus fermentum , Lactobacillus plantarum , Lactobacillus salivarius , Lactobacillus breve and Lactobacillus bifidus. The present invention provides a freeze-dried feminine hygiene product containing a manufactured complex fermented extract as an active ingredient.

Finally, another object of the present invention is to provide a method for manufacturing a freeze-dried feminine hygiene product.

To achieve the above purpose, the present invention provides a feminine hygiene product containing a composite fermented extract of mugwort, green tea, purslane and cactus stem as an effective ingredient.

In one embodiment of the present invention, the fermentation may be performed using, but is not limited to, one or more of Lactobacillus acidophilus , Lactobacillus rhamnosus , Lactobacillus paracasei , Lactobacillus casei, Lactobacillus bulgaricus , Lactobacillus fermentum , Lactobacillus plantarum , Lactobacillus salivarius , Lactobacillus breve , and Lactobacillus bifidus.

In one embodiment of the present invention, the fermentation may be a complex fermentation of Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum , Lactobacillus breve , and Lactobacillus bifidus , but is not limited thereto.

In one embodiment of the present invention, the feminine hygiene product may have antibacterial properties against, but is not limited to, Pseudomonas aeruginosa , Candida albicans , Escherichia coli, and Bacillus cereus .

In one embodiment of the present invention, the feminine hygiene product may be in a solid form through freeze-drying, but is not limited thereto.

In addition, the present invention provides a cosmetic composition for antibacterial use, comprising a composite fermented extract of mugwort, green tea, purslane, and cactus stem as an effective ingredient, wherein the fermentation is a composite fermentation of Lactobacillus rhamnosus, Lactobacillus paracasei , Lactobacillus plantarum , Lactobacillus breve , and Lactobacillus bifidus , and the antibacterial effect is against Pseudomonas aeruginosa , Candida albicans, Escherichia coli , and Bacillus cereus .

Lastly, the present invention provides a health functional food composition for antibacterial purposes, comprising a composite fermented extract of mugwort, green tea, purslane, and cactus stem as an effective ingredient, wherein the fermentation is a composite fermentation of Lactobacillus rhamnosus , Lactobacillus paracasei , Lactobacillus plantarum , Lactobacillus breve , and Lactobacillus bifidus , and the antibacterial effect is against Pseudomonas aeruginosa , Candida albicans , Escherichia coli, and Bacillus cereus .

The present invention relates to a freeze-dried feminine cleanser containing a natural extract as an active ingredient, and more particularly, to a freeze-dried feminine cleanser prepared by producing a complex fermented extract of mugwort ( Artemisia princeps ), green tea ( Camellia Sinensis Leaf), centella asiatica , and opuntia ficus - indica stem using Lactobacillus spp. and containing the same as an active ingredient. It was confirmed that the feminine cleanser exhibits excellent antibacterial properties against four types of harmful strains, Pseudomonas aeruginosa , Candida albicans, Escherichia coli, and Bacillus cereus .

Figure 1 is a diagram showing in chronological order how, in one embodiment of the present invention, the feminine hygiene product of the present invention is easily liquefied when water comes into contact with the air layer.

Hereinafter, the present invention will be described in detail with reference to the attached drawings as embodiments of the present invention. However, the following embodiments are presented as examples of the present invention, and if it is judged that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description thereof may be omitted, and the present invention is not limited thereby. The present invention is capable of various modifications and applications within the scope of the following claims and equivalents interpreted therefrom.

In addition, the terms used in this specification are terms used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator, or the customs of the field to which the present invention belongs. Therefore, the definitions of these terms should be made based on the contents throughout this specification. Throughout the specification, when a part is said to “include” a certain component, this does not mean that other components are excluded, but rather that other components can be further included, unless specifically stated otherwise.

Throughout this specification, '%' used to indicate the concentration of a particular substance, unless otherwise stated, is (w/w) % for solid/solid, (w/v) % for solid/liquid, and (v/v) % for liquid/liquid.

The extract according to the present invention can be obtained by extracting and separating from nature using extraction and separation methods known in the art, and the "extract" defined in the present invention is extracted from mugwort ( Artemisia princeps ), green tea ( Camellia Sinensis Leaf), centella asiatica , and Opuntia ficus - indica Stem using an appropriate solvent, and includes, for example, a crude extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract. Any organic solvent acceptable in cosmetics or pharmaceuticals may be used as a suitable solvent for extracting the above mugwort, green tea, purslane and bogum cactus stem complex extracts. Water or an organic solvent may be used, but is not limited thereto. For example, purified water; alcohols having 1 to 4 carbon atoms, including methanol, ethanol, propanol, isopropanol and butanol; acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in mixtures. As an extraction method, any one of the following methods may be selected and used: hot water extraction, cold immersion extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, dissolution, pressing, and supercritical extraction. In addition, the desired extract may additionally undergo a conventional fractionation process and may be purified using a conventional purification method.

There is no limitation on the method for producing the extract of the present invention, and any known method can be used. For example, the extract included in the composition of the present invention can be produced in a powder form by additional processes such as reduced pressure distillation and freeze drying or spray drying of the primary extract extracted by the above-mentioned hot water extraction or solvent extraction method. In addition, the primary extract can be obtained by further purifying fractions using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. Therefore, in the present invention, the extract is a concept including all extracts, fractions, and purified products obtained at each stage of extraction, fractionation, or purification, and their dilutions, concentrates, or dried products.

　

In one aspect, the present invention provides a freeze-dried feminine hygiene product comprising a composite extract or composite fermented extract of mugwort, green tea, purslane and cactus stem as an effective ingredient, which can be used as a cosmetic composition or an over-the-counter drug composition.

The feminine hygiene product of the present invention has excellent antibacterial properties against four types of harmful strains, Pseudomonas aeruginosa , Candida albicans , Escherichia coli, and Bacillus cereus . Products to which the present composition can be added include, for example, cosmetics such as feminine hygiene products, various creams, lotions, skins, and essences, as well as shampoos, rinses, cleansing agents, facial cleansers, soaps, treatments, packs, and beauty solutions.

The feminine hygiene product of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular peptides, high molecular polysaccharides, sphingolipids, and seaweed extracts.

Any water-soluble vitamin that can be mixed in cosmetics may be used, but preferably includes vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinamide, folic acid, vitamin C, vitamin H, etc., and their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbate-2-phosphate, magnesium ascorbate-2-phosphate, etc.) are also included in the water-soluble vitamins that can be used in the present invention. The water-soluble vitamins can be obtained by a conventional method such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method, or a chemical synthesis method.

Any useful vitamin that can be mixed in cosmetics may be used, but preferably includes vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and their derivatives (ascorbic acid palmitate, ascorbic acid stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.). The useful vitamins can be obtained by conventional methods such as microbial transformation, purification from a microbial culture, enzymatic or chemical synthesis, etc.

As the high molecular weight peptide, any polymer that can be mixed in cosmetics may be used, but preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, etc. can be used. The high molecular weight peptide can be purified and obtained by a conventional method, such as a purification method from a culture solution of a microorganism, an enzymatic method, or a chemical synthesis method, or can be purified and used from natural products, such as the dermis of pigs or cows, or silkworm fibers.

Any polymeric polysaccharide that can be mixed in cosmetics may be used, but preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be used. For example, chondroitin sulfate or a salt thereof can usually be purified from mammals or fish and used.

Any sphingolipid that can be mixed into cosmetics may be used, but preferably, ceramide, phytosphingosine, sphingoglycolipid, etc. Sphingolipid can be purified by a conventional method from mammals, fish, shellfish, yeast, or plants, or can be obtained by a chemical synthesis method.

Any seaweed extract that can be mixed in cosmetics may be used, but preferably, brown seaweed extract, red seaweed extract, green seaweed extract, etc. are used. In addition, calagenan, arginic acid, sodium arginate, potassium arginate, etc. purified from these seaweed extracts are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition to the above essential ingredients, the feminine hygiene product of the present invention may contain other ingredients commonly contained in cosmetics, if necessary. In addition, examples of ingredients that may be added include fat components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, sterilizers, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation promoters, cooling agents, antiperspirants, purified water, etc. Examples of fat components include ester-based fats, hydrocarbon-based fats, silicone-based fats, fluorine-based fats, animal fats, and plant fats.

As ester-based fats and oils, tri-2-ethylhexanoate glyceryl, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate, isoalkyl neopentanoate, tri(caprylic, capric)glyceryl, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, tetra-2-ethylhexanoate pentaellistolate, cetyl caprylate, decyl laurate, Hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di(capryl, capric acid) propylene glycol, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, Triundecyl glyceryl, triisopalmitate glyceryl, triisostearate glyceryl, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate ester, polyglycerol isostearate ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, hydroxystearic acid Examples thereof include esters such as 2-ethylhexyl, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesterol stearate, cholesterol isostearate, cholesterol hydroxystearate, cholesterol oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, and isostearyl 12-stearoylhydroxystearate.

Examples of hydrocarbon-based fats include squalene, liquid paraffin, alpha-olefin oligomers, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybdenum, microcrystalline wax, and vaseline.

Examples of silicone-based oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymers, dimethylsiloxane and methyl stearoxysiloxane copolymers, alkyl-modified silicone oils, and amino-modified silicone oils.

Examples of fluorinated oils include perfluoropolyether.

Examples of animal or plant fats include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, safflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, coconut oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, moonflower oil, margarine nut oil, meadowsweet oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rye oil, jojoba oil, candelilla wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of moisturizers include water-soluble low-molecular-weight moisturizers, fat-soluble molecular-weight moisturizers, water-soluble polymers, and fat-soluble polymers.

Examples of water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or higher), polypropylene glycol (polymerization degree n = 2 or higher), polyglycerin B (polymerization degree n = 2 or higher), lactic acid, and lactate salts.

Examples of fat-soluble low-molecular-weight moisturizers include cholesterol and cholesterol esters.

Examples of water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, etc.

Examples of lipid-soluble polymers include polyvinylpyrrolidone and eicosene copolymers, polyvinylpyrrolidone and hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, and high molecular silicones. Examples of emollients include long-chain acylglutamic acid cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Surfactants include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE and POP (polyoxyethylene and polyoxypropylene) copolymer, POE and POP alkyl ether, polyether-modified silicone, lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc.

Examples of anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkyl amide sulfates, alkyl phosphates, POE alkyl phosphorus acids, alkyl amide phosphates, alkyloyl alkyl taurine salts, N-acyl amino acids, POE alkyl ether carboxylates, alkyl sulfosuccinates, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphate esters.

Examples of cationic surfactants include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzylammonium chloride, behenyl trimethyl ammonium bromide, benzalkonium chloride, diethyl amino ethyl amide stearate, dimethyl amino propyl amide stearate, and quaternary ammonium salts of lanolin derivatives. Examples of amphoteric surfactants include carboxy betaine type, amide betaine type, sulfo betaine type, hydroxy sulfo betaine type, amide sulfo betaine type, phospho betaine type, aminocarboxylic acid salt type, imidazoline derivative type, and amideamine type.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and inorganic pigments such as complexes thereof; Examples thereof include organic pigments such as polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene and styrene copolymers, silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate; alkyl phosphate metal salts such as sodium zinc cetylphosphate, zinc laurylate, and calcium laurylate; acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; amide sulfonic acid polyvalent metal salts such as N-lauroyl-calcium taurine, and N-palmitoyl-calcium taurine; N-acyl basic amino acids such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoyl lysine, N-alpha-paritoyl olnithine, N-alpha-lauroyl arginine, and N-alpha-hydrogenated beef tallow fatty acid acylarginine; N-acyl polypeptides such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene and styrene copolymers, ethylene tetrafluoride, etc.

As ultraviolet absorbers, para-aminobenzoic acid, para-aminobenzoic acid ethyl, para-aminobenzoic acid amyl, para-aminobenzoic acid octyl, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamic acid, para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono-2-ethylhexaneglyceryl, para-methoxycinnamic acid isopropyl, diisopropyl and diisopropylcinnamic acid ester mixtures, urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, Examples include tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1,3,5-triazine, and 2-(2-hydroxy-5-methylphenyl)benzotriazole.

Examples of disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincpyrithione, benzalkonium chloride, photosensitive agent No. 301, sodium mononitroguaiachol, and undecylenic acid.

Antioxidants include butylated hydroxyanisole, propyl gallic acid, and ellisorbic acid.

pH adjusters include citric acid, sodium citrate, malic acid, sodium malate, formaldehyde, sodium formaldehyde, succinic acid, sodium succinate, sodium hydroxide, and sodium hydrogen phosphate.

As for alcohol, we can mention higher alcohols such as cetyl alcohol.

In addition, the mixing ingredients that may be added are not limited to these, and any of the above ingredients can be mixed within a range that does not impair the purpose and effect of the present invention.

The feminine hygiene product of the present invention can take the form of a solid, solution, emulsion, viscous mixture, etc., resulting from freeze-drying.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions as effective ingredients, and include, for example, conventional auxiliary agents and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances.

The feminine hygiene product comprising a composite extract or composite fermented extract of mugwort, green tea, safflower, and cactus stem of the present invention as an effective ingredient has excellent antibacterial activity against four types of harmful strains, Pseudomonas aeruginosa, Candida albicans, Escherichia coli, and Bacillus cereus, and can be used as a cosmetic composition. Such a cosmetic composition can be produced in any formulation commonly produced in the art, and examples thereof include a solid, an emulsion, a cream, a toner, a pack, a foundation, a lotion, a cosmetic solution, a hair cosmetic, etc.

Specifically, the cosmetic composition of the present invention includes formulations of feminine hygiene product, skin lotion, skin softener, skin toner, milk lotion, astringent, lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, hair lotion, hair tonic, hair essence, hair shampoo, hair rinse, hair treatment, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide can be used as a carrier component.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included.

When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used as carrier components.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative, or ethoxylated glycerol fatty acid ester may be used as a carrier component.

Hereinafter, the present invention will be described in more detail through examples. However, the above examples and experimental examples are presented as examples of the present invention, and if it is judged that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted, and the present invention is not limited thereby. The present invention can be variously modified and applied within the scope of the following claims and equivalents interpreted therefrom.

　

<Preparation Example 1> Manufacturing of Natural Complex Extract

In the present invention, mugwort ( Artemisia princeps ), green tea ( Camellia Sinensis Leaf), centella asiatica , and opuntia ficus - indica stem used in the manufacture of natural complex extracts were purchased from a herbal medicine market. When manufacturing an extract using the above materials, hot water extraction was performed using a stirrer at 150 rpm for 3 to 5 hours at 95 to 100°C, and the complex extracts extracted according to the above method were made into a powder form by removing all solvents through freeze-drying.

<Preparation Example 2> Manufacturing of Natural Complex Fermentation Extract

A natural complex fermented extract was prepared by fermentation using the complex extract prepared in the above Preparation Example 1. The strains used for fermentation were at least one of Lactobacillus acidophilus , Lactobacillus rhamnosus, Lactobacillus paracasei , Lactobacillus casei, Lactobacillus bulgaricus , Lactobacillus fermentum , Lactobacillus plantarum , Lactobacillus salivarius , Lactobacillus breve , and Lactobacillus bifidus. The above strains are strains provided by the "Food Microbial Gene Bank" of the Korea Food Research Institute or the Korea Microbial Conservation Center. The strain deposit numbers and deposit institutions are as shown in Table 1 below.

Strain name Accession number Depository Institution Lactobacillus acidophilus 40265 Korea Center for Microbiology (KCCM) Lactobacillus rhamnosus 40069 Korea Center for Microbiology (KCCM) Lactobacillus paracasei 40996 Korea Center for Microbiology (KCCM) Lactobacillus casei 12452 Korea Center for Microbiology (KCCM) Lactobacillus bulgaricus 40266 Korea Center for Microbiology (KCCM) Lactobacillus fermantum 35469 Korea Center for Microbiology (KCCM) Lactobacillus plantarum 60805 Korea Food Research Institute Lactobacillus salivarius 40210 Korea Center for Microbiology (KCCM) Lactobacillus breve 61605 Korea Food Research Institute Lactobacillus bifidus 12096 Korea Center for Microbiology (KCCM)

Pre-cultivation was performed by adding 10 mg of at least one of the Lactobacillus spp. to 10 mL of a fermentation medium (glucose 1.5% (v/v), sugar 1.5% (v/v), corn syrup 1.5% (v/v), yeast extract 0.3% (v/v)). After pre-cultivation, the natural complex extract was added to a medium having the same composition as the fermentation medium, and the pre-culture solution was inoculated and fermented. The culture was performed at 35 to 37°C for 36 hours, and the glucose and sugar contents were controlled to improve the growth rate of the strain during the culture. After the fermentation was completed, the culture solution and the strain bodies were separated through centrifugation and filtering, and the separated culture solution portion was freeze-dried to obtain the natural complex fermentation extract of the present invention.

<Experimental Example 1> Manufacturing of a comparative example for antibacterial effect test and its antibacterial effect

According to the manufacturing methods of Preparation Examples 1 and 2 above, a comparative experimental group of natural complex fermented extracts was prepared as shown in Table 2 below. For convenience, the strains of the genus Lactobacillus were referred to as Lactobacillus acidophilus (LA), Lactobacillus rhamnosus (LR), Lactobacillus paracasei (LP), Lactobacillus casei (LC), Lactobacillus bulgaricus (LB), Lactobacillus fermentum (LF), Lactobacillus plantarum (LPT), Lactobacillus salivarius (LS), and Lactobacillus breve (LBV), and Lactobacillus bifidus (LBV). It was named abbreviated as LBD.

LA LR LP LC LB LF LPT LS LBV LBD Comparative Example 1 O Comparative Example 2 O Comparative Example 3 O Comparative Example 4 O Comparative Example 5 O Comparative Example 6 O Comparative Example 7 O Comparative Example 8 O Comparative Example 9 O Comparative Example 10 O

Natural complex fermentation extracts according to the above Table 2 were prepared and an antibacterial test was conducted using them. The antibacterial effect was compared by wetting the comparative examples on a paper disc, placing them on a medium on which microorganisms were smeared, culturing them, and measuring the size of the clear zone around the disc. Specifically, 50 ㎕ of each comparative example was wetting a paper disc (diameter 8 mm, thickness 1.5 mm), and then leaving it at room temperature for one day. The four strains to be tested for antibacterial activity, Pseudomonas aeruginosa , Candida albicans, Escherichia coli , and Bacillus cereus, were cultured at 30°C or 37°C for 1 to 2 days, respectively, and the absorbance was measured and calculated to be 1× ¹⁰⁴ CFU. The four strains were then evenly spread on solid media (Nutrient Agar medium for Pseudomonas aeruginosa , Escherichia coli , and Bacillus cereus , and Sabrouraud Dextrose Agar medium for Candida albicans ) and cultured in an incubator at 37°C for 2 to 3 days. Thereafter, the antibacterial activity was confirmed by measuring the diameter of the transparent zone around the paper disk where no bacteria grew. The diameter of the transparent ring is expressed in mm, and the larger the diameter of the transparent ring, the higher the antibacterial power. The results of the antibacterial effect are as shown in Table 3 below.

Comparative example
/ (mm) 1 2 3 4 5 6 7 8 9 10 Pseudomonas aeruginosa 0.1 1.1 1.9 0.2 0.1 0.5 1.9 0.4 1.9 1.4 Candida albicans 0.3 1.5 1.1 0.1 0.8 0.4 1.1 0.5 1.7 1.5 Escherichia coli 0.2 1.8 1.2 0.8 0.9 0.1 1.2 0.7 1.8 1.4 Bacillus cereus 0.5 1.7 1.2 0.7 0.4 0.1 1.8 0.2 1.8 1.5

In Comparative Examples 1 to 10, it was confirmed that Comparative Examples 2 ( Lactobacillus rhamnosus ; LR), 3 (Lactobacillus paracasei; LP), 7 ( Lactobacillus plantarum; LPT ), 9 ( Lactobacillus breve ; LBV)) and 10 ( Lactobacillus bifidus ; LBD) had excellent antibacterial activity against four harmful strains, Pseudomonas aeruginosa, Candida albicans, Escherichia coli and Bacillus cereus.

<Experimental Example 2> Antibacterial effect of natural complex fermentation extract using various strains

Comparative examples 2, 3, 7, 9 and 10, which showed excellent antibacterial effects in the above experimental example 1, were used for complex fermentation and their antibacterial effects were measured. Using the above five comparative examples, examples were composed as shown in Table 4 below.

LR
(Comparative Example 2) LP
(Comparative Example 3) LPT
(Comparative Example 7) LBV
(Comparative Example 9) LBD
(Comparative Example 10) Example 1 O O Example 2 O O Example 3 O O Example 4 O O Example 5 O O O Example 6 O O O Example 7 O O O Example 8 O O O O Example 9 O O O O Example 10 O O O O O

As shown in Table 4 above, when multiple strains were added, all ratios were added equally and fermentation was conducted (for example, when fermenting two strains, they were added at a weight ratio of 1:1). The subsequent antibacterial effect test method was conducted according to the method of Experimental Example 1 above. The results of the antibacterial effect of the natural complex fermentation extract are as shown in Table 5 below.

Example
/ (mm) 1 2 3 4 5 6 7 8 9 10 Pseudomonas aeruginosa 0.8 1.1 1.5 1.1 1.1 1.5 1.1 2.4 1.8 2.0 Candida albicans 1.2 1.1 1.4 1.2 0.8 1.7 1.8 2.6 1.7 1.8 Escherichia coli 1.8 1.4 1.4 1.1 0.8 1.7 1.9 2.7 2.1 2.2 Bacillus cereus 2.1 0.5 1.8 0.8 1.7 1.8 0.7 2.2 1.9 1.7

As can be confirmed from the above experimental results, overall, the effect was shown to be better than the result of Experimental Example 1, which was the single fermentation, but due to the interaction between the strains, high antibacterial activity was shown only in the case of Example 8. Therefore, it was confirmed that the natural complex fermented extract using Lactobacillus rhamnosus (LR), Lactobacillus paracasei (LP), Lactobacillus plantarum (LPT), and Lactobacillus breve (LBV) of Example 8 can be used as a feminine hygiene product.

<Experimental Example 3> Freeze-drying of natural complex fermentation extract

A freeze-dried feminine hygiene product was manufactured using Example 8, which has high antibacterial activity confirmed in the above Experimental Example 2. As shown in Fig. 1, it was confirmed that it was easily liquefied when water came into contact with the air layer. Therefore, the feminine hygiene product of the present invention has excellent antibacterial activity, is comfortable to use because it dissolves immediately upon contact with water, is convenient to carry around, and is human-friendly because it does not contain preservatives.

As described above, the present invention has been described with the above-mentioned preferred embodiments, but those skilled in the art will be able to easily suggest other backward inventions or other embodiments included within the scope of the present invention by adding, changing, deleting, etc. other components within the same technical scope. Therefore, the embodiments described above are exemplary in all aspects and do not limit the scope of the present invention.

Claims (1)

Contains mugwort, green tea, purslane, and cactus stem complex fermented extracts as active ingredients.
The above fermentation was performed using Lactobacillus rhamnosus (LR), Lactobacillus paracasei (LP), Lactobacillus plantarum (LPT), and Lactobacillus breve (LBV).
It has antibacterial activity against Pseudomonas aeruginosa , Candida albicans and Escherichia coli .
It is in solid form through freeze-drying.
A freeze-dried feminine wash that does not contain preservatives.