KR102687822B1 - Biodegradable polymer microsphere for reducing wrinkle and improving elasticity containing hyaluronic acid(HA), PCL, PLA, PLLA, PLGA and PDO, and method for manufacturing the same - Google Patents

Abstract

The biodegradable polymer microspheres according to one aspect of the present invention are hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO in a ratio of 1: 0.1 to 0.4: 0.05 to 0.2: 0.15 to 0.6: 0.1 to 0.4: 0.1 to 0.4. By including biodegradable polymer microspheres by weight as active ingredients, PCL, PLA, PLLA, PLGA and PDO can be sequentially absorbed from the skin, allowing the effect of collagen expression to be maintained for a long time, immediately and continuously after use. It has excellent effects in reducing wrinkles and improving elasticity.

Description

Biodegradable polymer microspheres for reducing wrinkles and improving elasticity containing hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO and method for manufacturing the same {Biodegradable polymer microsphere for reducing wrinkle and improving elasticity containing hyaluronic acid(HA), PCL , PLA, PLLA, PLGA and PDO, and method for manufacturing the same}

The examples below relate to biodegradable polymer microspheres for reducing wrinkles and improving elasticity containing hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO, and methods for producing the same.

Among medical devices for cosmetic procedures sold for the purpose of cosmetic procedures, fillers are used by injecting into skin tissue in wrinkles and depressions.

Most fillers are injected into skin tissue to improve skin wrinkles or depressions by increasing the volume of skin tissue through the volume of the substance itself. Currently, the most commonly used fillers include gel-type intangible substances such as hyaluronic acid and collagen, but there is a problem in that they are absorbed into the skin tissue over time and the cosmetic treatment effect is not maintained for a long period of time.

Meanwhile, microsphere manufacturing technology for biodegradable polymers is a technology that is receiving great attention not only in the fields of plastic surgery and skin care, but also in the field of long-term sustained-release drug delivery through drug encapsulation.

However, many depot preparations using fillers and drug encapsulation using biodegradable polymer microspheres have been developed recently, but problems such as irregular particle shape and size or long time taken for collagen production in the skin are known. .

In addition, it takes a lot of time in the manufacturing process to produce microspheres of a certain size, and although most of them are biodegradable and absorbed in the body without side effects, a problem was discovered that causes side effects such as inflammatory reactions with a low probability, so additional research and development is needed. am.

Accordingly, the present inventors conducted research on microspheres for long-term wrinkle reduction and elasticity improvement, which are biodegradable polymer microspheres that can be used not only as filler components but also as components of cosmetic compositions, and completed the present invention.

KR 10-2181033 B1 KR 10-2407160 B1 KR 10-2172140 B1 KR 10-2018-0130344 A

The present inventors confirmed that microspheres containing hyaluronic acid, PCL, PLA, PLLA, PLGA, and PDO in a specific weight ratio have an immediate and continuous wrinkle reduction and elasticity improvement effect, and completed the present invention.

Accordingly, in one aspect, the purpose of the present invention is to provide biodegradable polymer microspheres that exhibit immediate and sustained wrinkle reduction and elasticity improvement effects.

One aspect of the present invention includes hyaluronic acid (HA), polycaprolactone (PCL), polylactic acid (PLA), and poly-L-lactic acid (PLLA). ), poly(lactic-co-glycolic acid, PLGA), and polydioxanone (PDO) as active ingredients, and the hyaluronic acid (HA), PCL, Provides biodegradable polymer microspheres for reducing wrinkles and improving elasticity, wherein the weight ratio of PLA, PLLA, PLGA and PDO is 1:0.1 to 0.4:0.05 to 0.2:0.15 to 0.6:0.1 to 0.4:0.1 to 0.4.

Another aspect of the present invention provides biodegradable polymer microspheres wherein the weight ratio of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO is 1:0.2:0.1:0.3:0.2:0.2.

Another aspect of the present invention provides biodegradable polymer microspheres, wherein the microspheres have a diameter of 1 to 150 μm.

Another aspect of the present invention is a method for producing biodegradable polymer microspheres, which includes hyaluronic acid (HA), polycaprolactone (PCL), polylactic acid (PLA), poly- Mix of L-lactic acid (poly-L-lactic acid, PLLA), poly(lactic-co-glycolic acid, PLGA), and polydioxanone (PDO) A step of preparing a biodegradable polymer solution by mixing the mixed solution with chloroform and stirring the polyvinyl alcohol solution; mixing the biodegradable polymer solution and the aqueous polyvinyl alcohol solution; preparing biodegradable polymer microspheres in the mixed solution by filtering them through a mesh made of silver threads and then performing sonication and stirring; hardening the biodegradable polymer microspheres by freeze-drying the mixed solution containing the biodegradable polymer microspheres; and washing the cured biodegradable polymer microspheres, applying a mixture of camellia oil and cactus flower extract to the biodegradable polymer microspheres, and then freeze-drying the biodegradable polymer microspheres.

Another aspect of the present invention is that the weight ratio of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO is 1: 0.1 to 0.4: 0.05 to 0.2: 0.15 to 0.6: 0.1 to 0.4: 0.1 to 0.4, Manufacturing method is provided.

The biodegradable polymer microspheres according to one aspect of the present invention are hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO in a ratio of 1: 0.1 to 0.4: 0.05 to 0.2: 0.15 to 0.6: 0.1 to 0.4: 0.1 to 0.4. By including biodegradable polymer microspheres by weight as active ingredients, PCL, PLA, PLLA, PLGA and PDO can be sequentially absorbed from the skin, allowing the effect of collagen expression to be maintained for a long time, immediately and continuously after use. It has excellent effects in reducing wrinkles and improving elasticity.

Hereinafter, embodiments will be described in detail with reference to the attached drawings. However, various changes can be made to the embodiments, so the scope of the patent application is not limited or limited by these embodiments. It should be understood that all changes, equivalents, or substitutes for the embodiments are included in the scope of rights.

Specific structural or functional descriptions of the embodiments are disclosed for illustrative purposes only and may be modified and implemented in various forms. Accordingly, the embodiments are not limited to the specific disclosed form, and the scope of the present specification includes changes, equivalents, or substitutes included in the technical spirit.

Terms such as first or second may be used to describe various components, but these terms should be interpreted only for the purpose of distinguishing one component from another component. For example, a first component may be named a second component, and similarly, the second component may also be named a first component.

The terms used in the examples are for descriptive purposes only and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that it does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by a person of ordinary skill in the technical field to which the embodiments belong. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted in an ideal or excessively formal sense unless explicitly defined in the present application. No.

The advantages and features of the present invention and methods for achieving them will become clear by referring to the embodiments described in detail below along with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below and will be implemented in various different forms. The present embodiments only serve to ensure that the disclosure of the present invention is complete and that common knowledge in the technical field to which the present invention pertains is not limited. It is provided to fully inform those who have the scope of the invention, and the present invention is only defined by the scope of the claims.

In the embodiments of the present invention, unless otherwise defined, all terms used herein, including technical or scientific terms, are the same as those commonly understood by a person of ordinary skill in the technical field to which the present invention pertains. It has meaning. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless clearly defined in the embodiments of the present invention, have an ideal or excessively formal meaning. It is not interpreted as

The shapes, sizes, proportions, angles, numbers, etc. disclosed in the drawings for explaining embodiments of the present invention are illustrative, and the present invention is not limited to the matters shown. Additionally, in describing the present invention, if it is determined that a detailed description of related known technologies may unnecessarily obscure the gist of the present invention, the detailed description will be omitted. When 'includes', 'has', 'consists of', etc. mentioned in the specification are used, other parts may be added unless 'only' is used. In cases where a component is expressed in the singular, the plural is included unless specifically stated otherwise.

When interpreting a component, it is interpreted to include the margin of error even if there is no separate explicit description.

Each feature of the various embodiments of the present invention can be partially or fully combined or combined with each other, and as can be fully understood by those skilled in the art, various technical interconnections and operations are possible, and each embodiment may be implemented independently of each other. It may be possible to conduct them together due to a related relationship.

In one aspect, the present invention relates to hyaluronic acid (HA), polycaprolactone (PCL), polylactic acid (PLA), and poly-L-lactic acid (PLLA). ), poly(lactic-co-glycolic acid, PLGA), and polydioxanone (PDO) as active ingredients, and the hyaluronic acid (HA), PCL, Provides biodegradable polymer microspheres for reducing wrinkles and improving elasticity, wherein the weight ratio of PLA, PLLA, PLGA and PDO is 1:0.1 to 0.4:0.05 to 0.2:0.15 to 0.6:0.1 to 0.4:0.1 to 0.4.

Biodegradable polymer microspheres according to one aspect of the present invention may include hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO. Specifically, the weight ratio of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO may be 1:0.1 to 0.4:0.05 to 0.2:0.15 to 0.6:0.1 to 0.4:0.1 to 0.4. More specifically, the weight ratio of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO may be 1:0.2:0.1:0.3:0.2:0.2.

The average diameter of the biodegradable polymer microspheres according to one aspect of the present invention may be 1 to 150 μm. Specifically, the average diameter of the biodegradable polymer microspheres is 1 μm or more, 10 μm or more, 20 μm or more, 30 μm or more, 40 μm or more, 50 μm or more, 60 μm or more, 70 μm or more, 80 μm or more, 90 μm or more. It may be 100 μm or more, 110 μm or more, 120 μm or more, 130 μm or more, or 140 μm or more, and 150 μm or less, 140 μm or less, 130 μm or less, 120 μm or less, 110 μm or less, 100 μm or less, 90 μm. It may be 80 μm or less, 70 μm or less, 60 μm or less, 50 μm or less, 40 μm or less, 30 μm or less, 20 μm or less, or 10 μm or less. More specifically, the average diameter of the biodegradable polymer microspheres is 75 μm or less. It may be μm.

Biodegradable polymer microspheres according to one aspect of the present invention may have a uniform size.

The biodegradable polymer microspheres according to one aspect of the present invention may be included as an active ingredient in pharmaceutical compositions, cosmetic compositions, or food compositions. The pharmaceutical composition or cosmetic composition may be a composition for filler, and the pharmaceutical composition, cosmetic composition, or food composition may be a composition for maintaining filler efficacy. Alternatively, the pharmaceutical composition, cosmetic composition, or food composition may include the biodegradable polymer microspheres as an active ingredient, specifically, if the biodegradability properties of the biodegradable polymer microspheres are utilized.

The biodegradable polymer microspheres or composition according to one aspect of the present invention may be used to reduce wrinkles and improve elasticity.

The composition according to one aspect of the present invention may include one or more types of emulsified, water-in-oil (W/O), oil-in-water (O/W), oil-dispersed, water-dispersed, and solubilized compositions.

The composition according to one aspect of the present invention includes lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, cream, sunscreen, nutritional cream, moisture cream, hand cream, foundation, essence, It may have one or more formulations selected from the group consisting of nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, suspension, gel, powder, paste, compact, mask pack or sheet, or aerosol. .

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and experimental examples. However, these examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited to these examples.

[Manufacturing example] Manufacturing a cosmetic composition for reducing wrinkles and improving elasticity

Hyaluronic acid (HA), polycaprolactone (PCL), polylactic acid (PLA), poly-L-lactic acid (PLLA), poly(lactic-co) -Produce biodegradable polymer pellets by mixing poly(lactic-co-glycolic acid, PLGA) and polydioxanone (PDO) in a weight ratio of 1:0.2:0.1:0.3:0.2:0.2. Then, 5% by weight of the biodegradable polymer pellets were mixed with 95% by weight of chloroform and stirred at a constant speed at 90°C to prepare a biodegradable polymer solution.

Next, 2% by weight of polyvinyl alcohol was mixed with 98% by weight of distilled water and stirred at a constant speed at 90°C to prepare an aqueous polyvinyl alcohol solution. Then, 15% by weight of the biodegradable polymer solution and 85% by weight of an aqueous polyvinyl alcohol solution were mixed, and the mixed solution was filtered through a #200 size mesh made of silver thread. Next, ultrasonic treatment and stirring were carried out simultaneously for 6 hours to emulsify the mixed solution, and fine polymer-chloroform particles were induced to be generated in the mixed solution to prepare biodegradable polymer microspheres. Afterwards, the mixed solution containing the biodegradable polymer microspheres was placed in a freeze dryer and freeze-dried to harden the biodegradable polymer microspheres. Next, the cured biodegradable polymer microspheres are washed with distilled water, and a mixture of 75% by weight of camellia oil and 25% by weight of cactus flower extract is applied to the cured biodegradable polymer microspheres and then freeze-dried to reduce wrinkles and Biodegradable polymer microspheres for improving elasticity were prepared (Example). The microspheres of the above example had a uniform size with an average diameter of about 75 μm.

Meanwhile, as a comparative example, a cosmetic composition was prepared in the same manner as in the above example, except for the step of filtering the mixture of the biodegradable polymer and the solution and the polyvinyl alcohol aqueous solution through a mesh before ultrasonic treatment and stirring. Comparative Example 2, prepared except for the step of washing the cured biodegradable polymer microspheres and then applying a mixture of camellia oil and cactus flower extract to the microspheres, the biodegradable polymer pellets were mixed with hyaluronic acid (HA), PCL, PLA, and PDO. Comparative Example 3 prepared by mixing at a weight ratio of 1:0.3:0.4:0.3, and the biodegradable polymer pellets were mixed with hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO at a weight ratio of 1:0.5:0.3:0.1: The cosmetic composition of Comparative Example 4, prepared by mixing at a weight ratio of 0.05:0.05, was additionally prepared.

[Experimental Example 1] Comparison of biocompatibility

To confirm the biocompatibility of the biodegradable polymer microspheres of Examples and Comparative Examples 1 to 4 prepared in the above Preparation Examples, 98% by weight of liquid culture medium was added to the medium in which human dermal fibroblast cells (HDF) were cultured. After adding a mixed culture containing 2% by weight of each microsphere, cell viability was checked. The cell viability of the fibroblast culture medium containing nothing was set to 100%, and the cell viability of the medium containing each Example and Comparison 1 to 4 after 24 hours was compared and shown in Table 1.

Untreated group Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Cell survival rate (%) 100 97 92 91 93 94

As shown in Table 1, it was confirmed that the cell viability of Examples and Comparative Examples 1 to 4 was more than 90%, confirming that the biodegradable polymer microspheres according to the production method according to one aspect of the present invention had excellent biocompatibility. In particular, when hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO were mixed at a weight ratio of 1:0.2:0.1:0.3:0.2:0.2, the cell viability was very excellent.

[Experimental Example 2] Comparison of collagen production levels

To confirm whether the biodegradable polymer microspheres of Examples and Comparative Examples 1 to 4 prepared in the above Preparation Example affect collagen production in actual cells, 98% by weight of liquid culture medium and each microparticle were added to the medium in which human dermal fibroblasts were cultured. 48 hours after adding 2% by weight of the mixed culture, the expression level of collagen production-inducing factors in each cell was confirmed by qPCR, and the results are shown in Table 2.

Untreated group Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Collagen production-inducing factor expression amount (%) 100 135 110 112 120 125

As shown in Table 2, the biodegradable polymer microspheres of Examples and Comparative Examples 1 to 4 showed increased expression of collagen production-inducing factors compared to the untreated group, and it can be seen that the amount of collagen production can increase when the actual cosmetic composition is used. there was. In particular, it shows. In particular, when hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO were mixed at a weight ratio of 1:0.2:0.1:0.3:0.2:0.2, the expression level of collagen production-inducing factor was very high, reaching 135%. As a result, even if biodegradable polymer microspheres are manufactured using the same method, they contain all hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO, and in particular, it is known that there is a difference in the improvement of collagen production depending on the weight ratio of the above ingredients. I was able to.

[Experimental Example 3] Evaluation of wrinkle reduction effect

The wrinkle reduction effect of the prepared Examples and Comparative Examples 1 to 4 was evaluated by the following method.

Specifically, subjects in their 20s or 30s were divided into 5 groups of 20 each, and the compositions containing the microspheres of Examples and Comparative Examples 1 to 4 were applied to the face for 4 months after washing with water once a day, at a temperature of 20 Skin wrinkles were photographed with VisioFace 1000D in an environment of 25°C to 25°C and humidity of 40 to 60%. Skin wrinkles (eyebrows, eyes, and sagging) were measured based on the photos taken above, and the measurement results of wrinkle differences were expressed in terms of volume, area, and area ratio, and the results are shown in Table 3 below. Here, volume represents the size and depth of wrinkles; the lower the value, the shallower the wrinkles. The area represents the width and length of the wrinkle, and the lower the value, the smaller the wrinkle, and the area ratio is the ratio of wrinkles, and the lower the value, the better the condition. The values in Table 3 below show the relative reduction rate based on the value measured on day 0 of application.

unit: % Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Wrinkle volume reduction rate 1 month later 8.00 6.80 6.74 7.04 7.54 2 months later 17.47 9.35 9.62 13.48 14.97 4 months later 25.72 15.71 15.85 21.71 22.27 Wrinkle area reduction rate 1 month later 7.19 5.55 5.81 6.02 6.31 2 months later 16.52 11.89 11.67 13.82 14.88 4 months later 23.29 13.74 13.97 19.25 20.36 Wrinkle area ratio reduction rate 1 month later 7.95 3.84 5.48 5.87 6.83 2 months later 15.82 5.15 7.86 9.82 14.68 4 months later 23.03 7.86 9.88 15.14 19.55

As shown in Table 3, hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO are included in a weight ratio of 1: 0.1 to 0.4: 0.05 to 0.2: 0.15 to 0.6: 0.1 to 0.4: 0.1 to 0.4. The cosmetic composition containing biodegradable polymer microspheres as an active ingredient has a high wrinkle volume reduction rate, wrinkle area reduction rate, and wrinkle area ratio reduction rate of 7 to 8% after 1 month of application, and the reduction rate is very large after 2 months and 4 months of application. It was confirmed that the wrinkle reduction effect was very excellent even after a long time after application.

On the other hand, Comparative Example 1 prepared without filtering the mixture of biodegradable polymer and solution and polyvinyl alcohol aqueous solution through a mesh before ultrasonic treatment and stirring, and the mixture of camellia oil and cactus flower extract were applied to the microspheres. In Comparative Example 2, which was manufactured without including the step, the wrinkle volume reduction rate, wrinkle area reduction rate, and wrinkle area ratio reduction rate after 1 month of application were all lower than those of the Example, and the degree of reduction did not significantly increase even after 2 and 4 months of application. The wrinkle reduction effect did not last long.

On the other hand, Comparative Example 3, in which the biodegradable polymer pellets contained only some components, and Comparative Example 4, in which the weight ratio of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO was different from the example, showed the wrinkle volume reduction rate after 1 month of application, There was no significant difference in the wrinkle area reduction rate and wrinkle area ratio reduction rate compared to the Example, but after 2 and 4 months of application, the degree of reduction in the above items was not as large as in the Example, so the wrinkle reduction effect was not as good as in the Example.

Considering that there is a difference in the biodegradation period of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO, the biodegradable polymer microspheres according to one aspect of the present invention and the composition containing the same as an active ingredient (implemented Example) is effective in maintaining collagen for a long time by producing collagen step by step after application to the skin, and it was found that the wrinkle reduction effect is excellent even after a long time after application.

[Experimental Example 4] Evaluation of elasticity improvement effect

The elasticity improvement effect of the prepared Examples and Comparative Examples 1 to 4 was evaluated by the following method.

Specifically, the subjects of Experimental Example 1 (subjects in their 20s or 30s) were divided into 5 groups of 20 people each, and the compositions containing the microspheres of Examples and Comparative Examples 1 to 4 were used, respectively, once a day for 4 months. did. Then, through a survey, the elasticity improvement effect and the feeling of use were each evaluated on a 5-point scale (very low improvement effect: 1 point, low: 2 points, average: 3 points, high: 4 points, very high: 5 points) ( The feeling of use was evaluated according to (very low: 1 point, slight: 2 points, average: 3 points, good: 4 points, very excellent: 5 points) and the average value was calculated, and the results are shown in Table 4 below.

Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Improved elasticity Immediate elasticity improvement 4.9 3.5 3.7 4.3 4.5 Continuous elasticity improvement 4.9 3.6 3.7 3.8 3.9

As shown in Table 4, hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO are included in a weight ratio of 1: 0.1 to 0.4: 0.05 to 0.2: 0.15 to 0.6: 0.1 to 0.4: 0.1 to 0.4. Users of the cosmetic composition containing biodegradable polymer microspheres as an active ingredient were able to perceive an immediate and continuous elasticity improvement effect.

On the other hand, Comparative Example 1 prepared without filtering the mixture of biodegradable polymer and solution and polyvinyl alcohol aqueous solution through a mesh before ultrasonic treatment and stirring, and the mixture of camellia oil and cactus flower extract were applied to the microspheres. Comparative Example 2, prepared without including the step, did not provide an immediate and continuous elasticity improvement effect.

On the other hand, Comparative Example 3, which contains only some components in the biodegradable polymer pellet, and Comparative Example 4, where the weight ratio of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO is different from the example, has an immediate elasticity improvement effect, but is not continuous. It was found that it was not.

Considering that there is a difference in the biodegradation period of hyaluronic acid (HA), PCL, PLA, PLLA, PLGA and PDO, the biodegradable polymer microspheres according to one aspect of the present invention, and the composition containing the same as an active ingredient (implemented Example) was confirmed to have an immediate and continuous elasticity improvement effect after application, and to provide a feeling of use with low stickiness, moisturizing power, and excellent adhesion.

Through this, the biodegradable polymer microspheres according to one aspect of the present invention contain hyaluronic acid (HA), PCL, PLA, PLLA, PLGA, and PDO at a ratio of 1: 0.1 to 0.4: 0.05 to 0.2: 0.15 to 0.6: 0.1 to 0.4: 0.1. It was found that by including biodegradable polymer microspheres at a weight ratio of 0.4 to 0.4 as an active ingredient, there was an excellent effect of reducing wrinkles and improving elasticity immediately and continuously after use.

Claims (3)

Hyaluronic acid, polycaprolactone (PCL), poly lactic acid (PLA), poly-L-lactic acid (PLLA), poly (lactic-co-glycolic acid) ) (Contains poly(lactic-co-glycolic acid, PLGA) and polydioxanone (PDO) as active ingredients,
The weight ratio of the hyaluronic acid, PCL, PLA, PLLA, PLGA and PDO is 1:0.1 to 0.4:0.05 to 0.2:0.15 to 0.6:0.1 to 0.4:0.1 to 0.4, biodegradable polymer microspheres for reducing wrinkles and improving elasticity.
According to paragraph 1,
The weight ratio of the hyaluronic acid, PCL, PLA, PLLA, PLGA and PDO is 1:0.2:0.1:0.3:0.2:0.2, and the average diameter of the microspheres is 1 to 150 μm, biodegradable polymer microspheres.
A method for producing biodegradable polymer microspheres according to any one of claims 1 to 2, wherein the production method includes
Hyaluronic acid contains polycaprolactone (PCL), polylactic acid (PLA), poly-L-lactic acid (PLLA), and poly(lactic-co-glycolic acid). ) (a biodegradable polymer solution preparation step of mixing poly(lactic-co-glycolic acid, PLGA), and polydioxanone (PDO), mixing the mixture with chloroform, and stirring;
Preparing a polyvinyl alcohol aqueous solution by mixing polyvinyl alcohol with distilled water and stirring;
Mixing the biodegradable polymer solution and the polyvinyl alcohol aqueous solution, filtering the mixed solution through a mesh made of silver thread, and then ultrasonicating and stirring to produce biodegradable polymer microspheres in the mixed solution;
Hardening the biodegradable polymer microspheres by freeze-drying the mixed solution containing the biodegradable polymer microspheres; and
A method for producing biodegradable polymer microspheres, comprising the step of washing the cured biodegradable polymer microspheres, applying a mixture of camellia oil and cactus flower extract to the biodegradable polymer microspheres, and then freeze-drying them.