KR102686874B1 - Cosmetic composition containing five whitening active ingredients and manufacturing method thereof - Google Patents

Abstract

The present invention provides a cosmetic composition for skin whitening and a method for producing the same.

Description

Cosmetic composition containing five whitening active ingredients and manufacturing method thereof {Cosmetic composition containing five whitening active ingredients and manufacturing method thereof}

The present invention relates to a cosmetic composition containing five whitening active ingredients and a method for producing the same.

In general, a person's skin color is significantly determined by the concentration and distribution of melanin in the skin, but is also affected by environmental or physiological conditions such as ultraviolet rays from the sun or fatigue and stress. Melanin is a general term for brown or black polymer pigments derived from oxidation of phenols by oxidase and is widely distributed in the animal and plant kingdom.

Melanin is produced through a non-enzymatic oxidation reaction after an enzyme called tyrosinase acts on tyrosine, a type of amino acid, to change it into DOPA and dopaquinone. However, although the path by which melanin is produced is known, the mechanism that induces melanin synthesis, which is a step prior to the action of tyrosinase, has not yet been revealed in detail.

Accordingly, conventionally, substances with tyrosinase inhibitory activity, such as hydroquinone, ascorbic acid, kojic acid, and glutathione, are mixed into ointments or cosmetics to treat skin whitening, freckles, and freckles. It has been used for the purpose of improving freckles. However, in the case of the above method, use was limited due to skin irritation or poor product stability, and as a result, the effect was weak. In addition, many of the whitening agents known to date have problems with toxicity or stability, so their use has been limited.

Patent Document 1: Republic of Korea Patent No. 10-2320424

The present invention was made to solve the above problems, and the purpose of the present invention is to provide a cosmetic composition for skin whitening with excellent skin whitening effect.

The object of the present invention is not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.

In order to achieve the above object, the present invention

Onion extract 2-4 parts by weight, polyglyceryl-6 behenate 0.6-0.8 parts by weight, hydrogenated poly (C6-14 olefin) 0.4-0.6 parts by weight, glycerin 2-4 parts by weight, panthenol 0.7-0.9 parts by weight , dipropylene glycol 0.4-0.6 parts by weight, caprylic/capric triglyceride 0.4-0.6 parts by weight, t-butyl alcohol 1.3-1.5 parts by weight, niacinamide 2-4 parts by weight, hydrogenated polydecene 0.04-0.06 parts by weight, polyacrylate crosspolymer-6 1.2-1.4 parts by weight, 1,2-hexanediol 0.3-0.5 parts by weight, disodium EDTA 0.04-0.06 parts by weight, glyceryl acrylate/acrylic acid Copolymer 1.7-1.9 parts by weight, sodium chloride 0.04-0.06 parts by weight, glyceryl stearate 0.6-0.8 parts by weight, sodium stearoyl glutamate 0.5-0.7 parts by weight, ethylhexylglycerin 0.5-0.7 parts by weight, damask rose callus Extracellular vesicles 0.6-0.8 parts by weight, allantoin 1-3 parts by weight, dipotassium glycyrrhizate 0.04-0.06 parts by weight, butylene glycol 1-3 parts by weight, hydrogenated lecithin 1-3 parts by weight, polyglyce Lil-10 stearate 0.3-0.5 parts by weight, beta-glucan 0.8-1.0 parts by weight, sodium ascorbyl phosphate 0.04-0.06 parts by weight, tocopherol 1-3 parts by weight, tranexamic acid 0.4-0.6 parts by weight, glutathione 0.4- 0.6 parts by weight, alpha-bisabolol 0.4-0.6 parts by weight, alpha-arbutin 0.4-0.6 parts by weight, 3-O-ethylascorbic acid 0.4-0.6 parts by weight, pyridoxine 0.4-0.6 parts by weight, folic acid 0.04-0.06 parts by weight, biotin 0.4-0.6 parts by weight, pentylene glycol 0.4-0.6 parts by weight, cyanocobalamin 0.04-0.06 parts by weight, xanthan gum 1-3 parts by weight, guar gum 1-3 parts by weight, fructooligosaccharide Lyde 0.4-0.6 parts by weight, Phytic Acid 0.04-0.06 parts by weight, Glucose 0.04-0.06 parts by weight, Hydrolyzed Pea Protein 0.4-0.6 parts by weight, Thiamine HCL 0.04-0.06 parts by weight, Riboflavin 0.7-0.9 parts by weight , linoleic acid 0.3-0.5 parts by weight, beta-carotene 1.5-1.7 parts by weight, bioflavonoid 0.04-0.06 parts by weight, sodium succinate 0.2-0.4 parts by weight, glycoprotein 0.2-0.4 parts by weight, and purified water 57.74-57.76. Provided is a cosmetic composition for skin whitening comprising parts by weight.

In addition, the cosmetic composition for skin whitening includes 1-3 parts by weight of fermented extract,

The fermented extract is,

Washing mulberry roots, pomegranate peels, and rhododendron stems and drying them at a temperature of 48-52°C for 5-7 hours; Heat treating dried mulberry roots, pomegranate peels, and rhubarb stems at a temperature of 118-122°C for 18-22 minutes; Grinding heat-treated mulberry roots, pomegranate peels, and red tree stems and mixing them at a weight ratio of 1:1:1 to prepare a mixed powder; Mixing 48-52 parts by weight of the mixed powder and 48-52 parts by weight of the bisuri extract, adding 0.3-0.7 parts by weight of cellulase, and performing an enzyme reaction at a temperature of 32-36°C for 2-4 days; After the enzyme reaction, heating to a temperature of 78-82°C and cooling to a temperature of 18-22°C; After cooling, adding 0.8-1.2 parts by weight of a strain containing Saccharomyces coreanus and fermenting at a temperature of 28-32°C for 2-4 days; After fermentation, adding 38-42 parts by weight of purified water and extracting at a temperature of 118-122°C for 8-10 hours to prepare an extract; and concentrating and freeze-drying the extract under reduced pressure.

The bisuri extract is,

Washing the raincoat and drying it at a temperature of 48-52°C for 5-7 hours; heat treating the dried non-suri at a temperature of 118-122°C for 18-22 minutes; Mixing 8-12 parts by weight of pulverized bisuri powder and 88-92 parts by weight of purified water, heating to a temperature of 98-102°C and boiling for 22-26 hours; and boiling and then filtering to obtain a filtrate.

In addition, the present invention

Washing the raincoat and drying it at a temperature of 48-52°C for 5-7 hours; heat treating the dried non-suri at a temperature of 118-122°C for 18-22 minutes; Mixing 8-12 parts by weight of pulverized bisuri powder and 88-92 parts by weight of purified water, heating to a temperature of 98-102°C and boiling for 22-26 hours; And after boiling, filtering to obtain a filtrate; Preparing a bisuri extract comprising a;

Washing mulberry roots, pomegranate peels, and rhododendron stems and drying them at a temperature of 48-52°C for 5-7 hours; Heat treating dried mulberry roots, pomegranate peels, and rhododendron stems at a temperature of 118-122°C for 18-22 minutes; Grinding heat-treated mulberry roots, pomegranate peels, and red tree stems and mixing them at a weight ratio of 1:1:1 to prepare a mixed powder; Mixing 48-52 parts by weight of the mixed powder and 48-52 parts by weight of the bisuri extract, adding 0.3-0.7 parts by weight of cellulase, and performing an enzyme reaction at a temperature of 32-36° C. for 2-4 days; After the enzyme reaction, heating to a temperature of 78-82°C and cooling to a temperature of 18-22°C; After cooling, adding 0.8-1.2 parts by weight of a strain containing Saccharomyces coreanus and fermenting at a temperature of 28-32°C for 2-4 days; After fermentation, adding 38-42 parts by weight of purified water and extracting at a temperature of 118-122°C for 8-10 hours to prepare an extract; Preparing a fermentation extract comprising; concentrating and freeze-drying the extract under reduced pressure; and

1.9-2.1 parts by weight of the fermented extract, 2-4 parts by weight of onion extract, 0.6-0.8 parts by weight of polyglyceryl-6 behenate, 0.4-0.6 parts by weight of hydrogenated poly(C6-14 olefin), 2-4 parts by weight of glycerin parts by weight, panthenol 0.7-0.9 parts by weight, dipropylene glycol 0.4-0.6 parts by weight, caprylic/capric triglyceride 0.4-0.6 parts by weight, t-butyl alcohol 1.3-1.5 parts by weight, niacinamide 2- 4 parts by weight, hydrogenated polydecene 0.04-0.06 parts by weight, polyacrylate crosspolymer-6 1.2-1.4 parts by weight, 1,2-hexanediol 0.3-0.5 parts by weight, disodium EDTA 0.04-0.06 parts by weight parts, glyceryl acrylate/acrylic acid copolymer 1.7-1.9 parts by weight, sodium chloride 0.04-0.06 parts by weight, glyceryl stearate 0.6-0.8 parts by weight, sodium stearoyl glutamate 0.5-0.7 parts by weight, ethylhexylglycerin 0.5 -0.7 parts by weight, damask rose callus extracellular vesicles 0.6-0.8 parts by weight, allantoin 1-3 parts by weight, dipotassium glycyrrhizate 0.04-0.06 parts by weight, butylene glycol 1-3 parts by weight, hydrogenated Lecithin 1-3 parts by weight, polyglyceryl-10 stearate 0.3-0.5 parts by weight, beta-glucan 0.8-1.0 parts by weight, sodium ascorbyl phosphate 0.04-0.06 parts by weight, tocopherol 1-3 parts by weight, tranexamic acid 0.4-0.6 parts by weight, glutathione 0.4-0.6 parts by weight, alpha-bisabolol 0.4-0.6 parts by weight, alpha-arbutin 0.4-0.6 parts by weight, 3-O-ethylascorbic acid 0.4-0.6 parts by weight, pyridoxine 0.4- 0.6 parts by weight, Folic acid 0.04-0.06 parts by weight, Biotin 0.4-0.6 parts by weight, Pentylene glycol 0.4-0.6 parts by weight, Cyanocobalamin 0.04-0.06 parts by weight, Xanthan gum 1-3 parts by weight, Guar gum 1 -3 parts by weight, fructooligosaccharide 0.4-0.6 parts by weight, phytic acid 0.04-0.06 parts by weight, glucose 0.04-0.06 parts by weight, hydrolyzed pea protein 0.4-0.6 parts by weight, thiamine HCL 0.04-0.06 Parts by weight, riboflavin 0.7-0.9 parts by weight, linoleic acid 0.3-0.5 parts by weight, beta-carotene 1.5-1.7 parts by weight, bioflavonoids 0.04-0.06 parts by weight, sodium succinate 0.2-0.4 parts by weight, glycoprotein 0.2 It provides a method for producing a cosmetic composition for skin whitening comprising the step of mixing -0.4 parts by weight and 57.74-57.76 parts by weight of purified water.

The cosmetic composition for skin whitening according to the present invention exhibits a skin whitening effect.

In addition, the ingredients included in the cosmetic composition for skin whitening according to the present invention have excellent functionality due to increased total polyphenol content and total flavonoid content.

Hereinafter, various embodiments will be described in more detail. The embodiments described herein may be modified in various ways. Specific embodiments may be described in detail in the detailed description. However, the specific embodiments disclosed are only intended to facilitate understanding of the various embodiments. Accordingly, the technical idea is not limited to the specific embodiments disclosed, and should be understood to include all equivalents or substitutes included in the spirit and technical scope of the invention.

Terms containing ordinal numbers, such as primary, secondary, first, second, etc., may be used to describe various components, but these components are not limited by the above-mentioned terms. The above-mentioned terms are used only for the purpose of distinguishing one component from another.

In this specification, terms such as 'include' or 'have' are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that it does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof. When a component is said to be 'connected' or 'connected' to another component, it is understood that it may be directly connected or connected to the other component, but that other components may exist in between. It should be. On the other hand, when it is mentioned that a component is 'directly connected' or 'directly connected' to another component, it should be understood that there are no other components in between.

In addition, when describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof is abbreviated or omitted.

This invention

Onion extract 2-4 parts by weight, polyglyceryl-6 behenate 0.6-0.8 parts by weight, hydrogenated poly (C6-14 olefin) 0.4-0.6 parts by weight, glycerin 2-4 parts by weight, panthenol 0.7-0.9 parts by weight , dipropylene glycol 0.4-0.6 parts by weight, caprylic/capric triglyceride 0.4-0.6 parts by weight, t-butyl alcohol 1.3-1.5 parts by weight, niacinamide 2-4 parts by weight, hydrogenated polydecene 0.04-0.06 parts by weight, polyacrylate crosspolymer-6 1.2-1.4 parts by weight, 1,2-hexanediol 0.3-0.5 parts by weight, disodium EDTA 0.04-0.06 parts by weight, glyceryl acrylate/acrylic acid Copolymer 1.7-1.9 parts by weight, sodium chloride 0.04-0.06 parts by weight, glyceryl stearate 0.6-0.8 parts by weight, sodium stearoyl glutamate 0.5-0.7 parts by weight, ethylhexylglycerin 0.5-0.7 parts by weight, damask rose callus Extracellular vesicles 0.6-0.8 parts by weight, allantoin 1-3 parts by weight, dipotassium glycyrrhizate 0.04-0.06 parts by weight, butylene glycol 1-3 parts by weight, hydrogenated lecithin 1-3 parts by weight, polyglyce Lil-10 stearate 0.3-0.5 parts by weight, beta-glucan 0.8-1.0 parts by weight, sodium ascorbyl phosphate 0.04-0.06 parts by weight, tocopherol 1-3 parts by weight, tranexamic acid 0.4-0.6 parts by weight, glutathione 0.4- 0.6 parts by weight, alpha-bisabolol 0.4-0.6 parts by weight, alpha-arbutin 0.4-0.6 parts by weight, 3-O-ethylascorbic acid 0.4-0.6 parts by weight, pyridoxine 0.4-0.6 parts by weight, folic acid 0.04-0.06 parts by weight, biotin 0.4-0.6 parts by weight, pentylene glycol 0.4-0.6 parts by weight, cyanocobalamin 0.04-0.06 parts by weight, xanthan gum 1-3 parts by weight, guar gum 1-3 parts by weight, fructooligosaccharide Lyde 0.4-0.6 parts by weight, Phytic Acid 0.04-0.06 parts by weight, Glucose 0.04-0.06 parts by weight, Hydrolyzed Pea Protein 0.4-0.6 parts by weight, Thiamine HCL 0.04-0.06 parts by weight, Riboflavin 0.7-0.9 parts by weight , linoleic acid 0.3-0.5 parts by weight, beta-carotene 1.5-1.7 parts by weight, bioflavonoid 0.04-0.06 parts by weight, sodium succinate 0.2-0.4 parts by weight, glycoprotein 0.2-0.4 parts by weight, and purified water 57.74-57.76. Provided is a cosmetic composition for skin whitening comprising parts by weight.

Hereinafter, the cosmetic composition for skin whitening according to the present invention will be described in detail.

The onion extract is extracted from onions, and onions (Allium cepa) are aquatic plants of the monocotyledonous plant Liliaceae family. Onion varieties are divided into those with round and flat scales, and those with red, yellow, and white scales. The scales of onions are mainly used as food, and the unique smell emitted from the scales is known to be due to compounds such as propyl disulfide and allyl sulfide. These compounds physiologically promote the secretion of digestive juices and have effects such as excitement, sweating, or diuresis. It is reported that there is. In addition, the onion scales are rich in polyphenols and contain a large amount of various vitamins, minerals such as calcium and phosphoric acid, and active ingredients such as quercetin, and are known to have excellent physiological effects such as removing harmful substances from the blood. Functionality can be secured by including the onion extract.

Glycerin is extracted from palm oil or palm oil, and is a colorless, odorless, highly viscous water-soluble liquid. Glycerin has the property of attracting moisture, so when applied to the skin, it helps the epidermis retain moisture, making the skin soft and smooth, and has a soothing effect. As such, glycerin has excellent moisturizing properties and is not toxic to the skin.

Panthenol is a derivative of vitamin B5, and when it enters the body, it changes into vitamin B5, which helps skin regeneration. It is also called 'provitamin B5'. It has excellent skin moisturizing and skin soothing effects, providing moisture that dry skin lacks. It is supplied directly and plays a role in relieving itchiness and redness.

Niacinamide is a type of niacin, a member of the vitamin B group. Niacinamide is a water-soluble vitamin B3, also known as nicotinamide. Applying niacinamide enables the activation of many enzymes related to melanin synthesis and skin brightness, making it effective in improving skin.

Damask rose callus extracellular vesicles are extracellular vesicles isolated from the growth medium from which the callus of Rosa damascena was cultured and the cells were removed. Shows moisturizing effect.

Allantoin is an ingredient obtained by extracting and purifying comfrey roots. It does not cause skin allergies and is effective in moisturizing the skin.

Hydrogenated lecithin is a structure in which hydrogen is added to the double bond of the alkyl group to form a single bond in the structure of lecithin. If the alkyl group double bond remains, it is called partial hydrogenation, but generally refers to a structure in which the alkyl group is a single bond. Applying the above-mentioned hydrogenated lecithin reduces dry skin and the formation of dead skin cells and restores elasticity.

Beta-Glucan is a type of polysaccharide that suppresses the proliferation and recurrence of cancer cells by activating the immune function of normal human cell tissue and contains interleukin and interferon that activate the function of immune cells. promotes creation.

Tocopherol, called vitamin E, is efficient in inhibiting the oxidation of polyunsaturated fatty acids, which make up all biological membranes that make up cells, the basic unit of the body, and plays an important role in preventing lipid peroxidation. . Tocopherol is a fat-soluble antioxidant that protects biological membranes by being oxidized first instead of polyunsaturated fatty acids that are easily oxidized by oxygen or oxygen-containing oxidation-promoting substances that exist in cell membranes. Tocopherol prevents the generation of free radicals that destroy skin cell membranes through an antioxidant effect similar to beta-carotene, thereby suppressing damage to collagen and elastic fibers and the occurrence of pigmentation.

Tranexamic acid is a synthetic amino acid and is a white crystal derived from the amino acid lysine. The efficacy of tranexamic acid is that when a large amount of prostaglandin E2 is produced in the skin's epidermal cells, it stimulates tyrosinase and increases melanin production. Tranexamic acid is effective in whitening skin and improving blemishes by inhibiting the production of prostaglandin E2.

Glutathione is in the form of a tripeptide made up of glutamic acid, cysteine, and glycine, which are amino acids, and is a reduced substance that absorbs oxygen radicals. The glutathione effectively removes radicals in the body and can achieve a whitening effect by inhibiting tyrosine, which is involved in the melanin synthesis process.

Alpha-bisabolol, alpha-arbutin, 3-O-ethylascorbic acid, pyridoxine, folic acid, biotin, pentylene glycol, cyanocobalamin, fructooligosaccharide, glucose, hydrolyzed pea protein. , riboflavin, linoleic acid, beta-carotene, bioflavonoids, sodium succinate and glycoprotein are applied as whitening aids.

In addition, the cosmetic composition for skin whitening preferably contains 1-3 parts by weight of fermented extract, and more preferably contains 1.5-2.5 parts by weight.

The fermented extract is,

Washing mulberry roots, pomegranate peels, and rhododendron stems and drying them at a temperature of 48-52°C for 5-7 hours; Heat treating dried mulberry roots, pomegranate peels, and rhubarb stems at a temperature of 118-122°C for 18-22 minutes; Grinding heat-treated mulberry roots, pomegranate peels, and red tree stems and mixing them at a weight ratio of 1:1:1 to prepare a mixed powder; Mixing 48-52 parts by weight of the mixed powder and 48-52 parts by weight of the bisuri extract, adding 0.3-0.7 parts by weight of cellulase, and performing an enzyme reaction at a temperature of 32-36°C for 2-4 days; After the enzyme reaction, heating to a temperature of 78-82°C and cooling to a temperature of 18-22°C; After cooling, adding 0.8-1.2 parts by weight of a strain containing Saccharomyces coreanus and fermenting at a temperature of 28-32°C for 2-4 days; After fermentation, adding 38-42 parts by weight of purified water and extracting at a temperature of 118-122°C for 8-10 hours to prepare an extract; and concentrating and freeze-drying the extract under reduced pressure.

Mulberry is a deciduous broad-leaved tree, and its active ingredients include mulverin, mulverrochromene, cyclomulverin, cyclomuberrochromene, scoporetin, umbelliferone, trigonelline, tannin, and the flavonoids morusin and tree. Terpenoids include α-β-amyrin, sitosterol, sitosterol-d-glucoside, betulinic acid, adenine, betaine, palmitic acid, and stearic acid. The present invention provides a fermented extract using mulberry roots.

Pomegranate (Punicagranatum L.) is a native plant of southwestern Asia, northwestern India, and California, USA, and is now widely spread throughout the subtropics and tropics. Pomegranate, especially red pomegranate, has been known as a tonic since ancient times, and is known to be particularly effective in preventing high blood pressure and arteriosclerosis. In addition, it contains a large amount of water-soluble carbohydrates (38 to 47%) and various vitamins and minerals. The present invention provides a fermented extract using pomegranate peel.

Rhys javanica L. is a deciduous shrub belonging to the lacquer family and grows wild in mountains all over the country. Although it belongs to the lacquer tree, it is not toxic. In oriental medicine, it has astringent, hemostatic, detoxifying, and antibacterial effects, and is prescribed for diarrhea, prolapse, gastric ulcer, duodenal ulcer, night sweats, oil well, hematochezia, hematuria, stomatitis, etc. In addition, the roots and leaves of the red tree are called saltwood and have been used for fever relief, detoxification, bruises, and hemostasis. The present invention provides a fermented extract using red tree stems.

In the present invention, the fermentation extract prepared using the mulberry root, pomegranate peel, and rhubarb stem is intended to be applied to a cosmetic composition for skin whitening, and the fermentation extract contains the active ingredients polyphenols and flavonoids through a fermentation process using bisuri extract. It has high functionality by extracting and applying it with a significantly increased content.

In addition, after the primary enzyme reaction using cellulase, the content of polyphenols and flavonoids, which are active ingredients, can be increased by performing secondary fermentation using Saccharomyces corianus strains.

The bisuri extract is,

Washing the raincoat and drying it at a temperature of 48-52°C for 5-7 hours; heat treating the dried non-suri at a temperature of 118-122°C for 18-22 minutes; Mixing 8-12 parts by weight of pulverized bisuri powder and 88-92 parts by weight of purified water, heating to a temperature of 98-102°C and boiling for 22-26 hours; and boiling and then filtering to obtain a filtrate; use the product prepared by performing the following.

Lespedeza cuneata G. Don is a perennial plant belonging to the genus Lespedeza of the leguminosae family. It is called by various names such as night gate and trefoil, and is used in the folk medicine to treat cough, asthma, lack of energy, etc. come. The whole plant, including the root, is used for medicinal purposes, and the physiologically active substances of night gate include pinitol, flavonoid, and phenol, which have excellent free radicals scavenging and antioxidant effects, and are effective in eliminating toxicity and Since it has no side effects, it is widely used as a cosmetic composition for anti-aging purposes.

In the present invention, the bisuri extract extracted using the bisuri plant can be applied to a fermentation process to increase the content of polyphenols and flavonoids, which are active ingredients inherent in mulberry roots, pomegranate peels, and rhododendron stems.

In addition, the present invention

Washing the raincoat and drying it at a temperature of 48-52°C for 5-7 hours; heat treating the dried non-suri at a temperature of 118-122°C for 18-22 minutes; Mixing 8-12 parts by weight of pulverized bisuri powder and 88-92 parts by weight of purified water, heating to a temperature of 98-102°C and boiling for 22-26 hours; And after boiling, filtering to obtain a filtrate; Preparing a bisuri extract comprising a;

Washing mulberry roots, pomegranate peels, and rhododendron stems and drying them at a temperature of 48-52°C for 5-7 hours; Heat treating dried mulberry roots, pomegranate peels, and rhododendron stems at a temperature of 118-122°C for 18-22 minutes; Grinding heat-treated mulberry roots, pomegranate peels, and red tree stems and mixing them at a weight ratio of 1:1:1 to prepare a mixed powder; Mixing 48-52 parts by weight of the mixed powder and 48-52 parts by weight of the bisuri extract, adding 0.3-0.7 parts by weight of cellulase, and performing an enzyme reaction at a temperature of 32-36° C. for 2-4 days; After the enzyme reaction, heating to a temperature of 78-82°C and cooling to a temperature of 18-22°C; After cooling, adding 0.8-1.2 parts by weight of a strain containing Saccharomyces coreanus and fermenting at a temperature of 28-32°C for 2-4 days; After fermentation, adding 38-42 parts by weight of purified water and extracting at a temperature of 118-122°C for 8-10 hours to prepare an extract; Preparing a fermentation extract comprising; concentrating and freeze-drying the extract under reduced pressure; and

1.9-2.1 parts by weight of the fermented extract, 2-4 parts by weight of onion extract, 0.6-0.8 parts by weight of polyglyceryl-6 behenate, 0.4-0.6 parts by weight of hydrogenated poly(C6-14 olefin), 2-4 parts by weight of glycerin parts by weight, panthenol 0.7-0.9 parts by weight, dipropylene glycol 0.4-0.6 parts by weight, caprylic/capric triglyceride 0.4-0.6 parts by weight, t-butyl alcohol 1.3-1.5 parts by weight, niacinamide 2- 4 parts by weight, hydrogenated polydecene 0.04-0.06 parts by weight, polyacrylate crosspolymer-6 1.2-1.4 parts by weight, 1,2-hexanediol 0.3-0.5 parts by weight, disodium EDTA 0.04-0.06 parts by weight parts, glyceryl acrylate/acrylic acid copolymer 1.7-1.9 parts by weight, sodium chloride 0.04-0.06 parts by weight, glyceryl stearate 0.6-0.8 parts by weight, sodium stearoyl glutamate 0.5-0.7 parts by weight, ethylhexylglycerin 0.5 -0.7 parts by weight, damask rose callus extracellular vesicles 0.6-0.8 parts by weight, allantoin 1-3 parts by weight, dipotassium glycyrrhizate 0.04-0.06 parts by weight, butylene glycol 1-3 parts by weight, hydrogenated Lecithin 1-3 parts by weight, polyglyceryl-10 stearate 0.3-0.5 parts by weight, beta-glucan 0.8-1.0 parts by weight, sodium ascorbyl phosphate 0.04-0.06 parts by weight, tocopherol 1-3 parts by weight, tranexamic acid 0.4-0.6 parts by weight, glutathione 0.4-0.6 parts by weight, alpha-bisabolol 0.4-0.6 parts by weight, alpha-arbutin 0.4-0.6 parts by weight, 3-O-ethylascorbic acid 0.4-0.6 parts by weight, pyridoxine 0.4- 0.6 parts by weight, Folic acid 0.04-0.06 parts by weight, Biotin 0.4-0.6 parts by weight, Pentylene glycol 0.4-0.6 parts by weight, Cyanocobalamin 0.04-0.06 parts by weight, Xanthan gum 1-3 parts by weight, Guar gum 1 -3 parts by weight, fructooligosaccharide 0.4-0.6 parts by weight, phytic acid 0.04-0.06 parts by weight, glucose 0.04-0.06 parts by weight, hydrolyzed pea protein 0.4-0.6 parts by weight, thiamine HCL 0.04-0.06 Parts by weight, riboflavin 0.7-0.9 parts by weight, linoleic acid 0.3-0.5 parts by weight, beta-carotene 1.5-1.7 parts by weight, bioflavonoids 0.04-0.06 parts by weight, sodium succinate 0.2-0.4 parts by weight, glycoprotein 0.2 It provides a method for producing a cosmetic composition for skin whitening comprising the step of mixing -0.4 parts by weight and 57.74-57.76 parts by weight of purified water.

Hereinafter, the present invention will be explained in more detail by the following examples.

However, the following examples only illustrate the content of the present invention and the scope of the invention is not limited by the examples and experimental examples.

<Preparation Example 1> Preparation of fermented extract-1

After washing the mulberry roots, pomegranate peels, and rhubarb stems and drying them at a temperature of 50℃ for 6 hours, heat-treating the dried mulberry roots, pomegranate peels, and rhubarb stems for 20 minutes at a temperature of 120℃, heat-treated mulberry roots, and pomegranates. The bark and red tree trunk were crushed and mixed at a weight ratio of 1:1:1 to prepare a mixed powder. 50 parts by weight of the mixed powder and 50 parts by weight of purified water were mixed, 1 part by weight of Saccharomyces coreanus strain was added, and fermented at a temperature of 30° C. for 3 days. After fermentation, 40 parts by weight of purified water was added, and extraction was performed at a temperature of 120°C for 9 hours to prepare an extract. The extract was concentrated under reduced pressure and freeze-dried to prepare a fermented extract.

<Preparation Example 2> Preparation of fermented extract-2

After washing the mulberry roots, pomegranate peels, and rhubarb stems and drying them at a temperature of 50℃ for 6 hours, heat-treating the dried mulberry roots, pomegranate peels, and rhubarb stems for 20 minutes at a temperature of 120℃, heat-treated mulberry roots, and pomegranates. The bark and red tree trunk were crushed and mixed at a weight ratio of 1:1:1 to prepare a mixed powder. 50 parts by weight of the mixed powder and 50 parts by weight of purified water were mixed, 0.5 parts by weight of cellulase was added, and the enzyme reaction was performed at a temperature of 34° C. for 3 days. After the enzyme reaction was terminated by heating to a temperature of 80°C, it was cooled to a temperature of 20°C, 1 part by weight of Saccharomyces coreanus strain was added, and fermentation was carried out at a temperature of 30°C for 3 days. After fermentation, 40 parts by weight of purified water was added, and extraction was performed at a temperature of 120°C for 9 hours to prepare an extract. The extract was concentrated under reduced pressure and freeze-dried to prepare a fermented extract.

<Preparation Example 3> Preparation of fermented extract-3

The non-suri was washed and dried at a temperature of 40°C for 6 hours. After heat-treating the dried bisuri at a temperature of 120°C for 20 minutes, the heat-treated bisuri was pulverized to prepare bisuri powder. A bisuri extract was prepared by mixing 10 parts by weight of the above-described non-suri powder and 90 parts by weight of purified water, heated to a temperature of 100°C, boiled for 24 hours, and filtered to obtain a filtrate.

After washing the mulberry roots, pomegranate peels, and rhubarb stems and drying them at a temperature of 50℃ for 6 hours, heat-treating the dried mulberry roots, pomegranate peels, and rhubarb stems for 20 minutes at a temperature of 120℃, heat-treated mulberry roots, and pomegranates. The bark and red tree trunk were crushed and mixed at a weight ratio of 1:1:1 to prepare a mixed powder. 50 parts by weight of the mixed powder and 50 parts by weight of the bisuri extract were mixed, 0.5 parts by weight of cellulase was added, and the enzyme reaction was performed at a temperature of 34° C. for 3 days. After the enzyme reaction was terminated by heating to a temperature of 80°C, it was cooled to a temperature of 20°C, 1 part by weight of Saccharomyces coreanus strain was added, and fermentation was carried out at a temperature of 30°C for 3 days. After fermentation, 40 parts by weight of purified water was added, and extraction was performed at a temperature of 120°C for 9 hours to prepare an extract. The extract was concentrated under reduced pressure and freeze-dried to prepare a fermented extract.

<Example 1> Preparation of cosmetic composition for skin whitening-1

3 parts by weight of onion extract, 0.7 parts by weight of polyglyceryl-6 behenate, 0.5 parts by weight of hydrogenated poly (C6-14 olefin), 3 parts by weight of glycerin, 0.8 parts by weight of panthenol, 0.5 parts by weight of dipropylene glycol, 0.5 parts by weight of caprylic/capric triglyceride, 1.4 parts by weight of t-butyl alcohol, 3 parts by weight of niacinamide, 0.05 parts by weight of hydrogenated polydecene, 1.3 parts by weight of polyacrylate crosspolymer-6, 1,2 parts by weight -Hexanediol 0.4 parts by weight, Disodium EDTA 0.05 parts by weight, Glyceryl acrylate/acrylic acid copolymer 1.8 parts by weight, Sodium chloride 0.05 parts by weight, Glyceryl stearate 0.7 parts by weight, Sodium stearoyl glutamate 0.6 Parts by weight, 0.6 parts by weight of ethylhexylglycerin, 0.7 parts by weight of Damask rose callus extracellular vesicles, 2 parts by weight of allantoin, 0.05 parts by weight of dipotassium glycyrrhizate, 2 parts by weight of butylene glycol, 2 parts by weight of hydrogenated lecithin. parts, polyglyceryl-10 stearate 0.4 parts by weight, beta-glucan 0.9 parts by weight, sodium ascorbyl phosphate 0.05 parts by weight, tocopherol 2 parts by weight, tranexamic acid 0.5 parts by weight, glutathione 0.5 parts by weight, alpha-bisabo 0.5 part by weight of roll, 0.5 part by weight of alpha-arbutin, 0.5 part by weight of 3-O-ethylascorbic acid, 0.5 part by weight of pyridoxine, 0.05 part by weight of polyacid, 0.5 part by weight of biotin, 0.5 part by weight of pentylene glycol, cyano Cobalamin 0.05 parts by weight, xanthan gum 2 parts by weight, guar gum 2 parts by weight, fructooligosaccharide 0.5 parts by weight, phytic acid 0.05 parts by weight, glucose 0.05 parts by weight, hydrolyzed pea protein 0.5 parts by weight, thiamine 0.05 parts by weight of HCL, 0.8 parts by weight of riboflavin, 0.4 parts by weight of linoleic acid, 1.6 parts by weight of beta-carotene, 0.05 parts by weight of bioflavonoids, 0.3 parts by weight of sodium succinate, 0.3 parts by weight of glycoprotein and 57.75 parts by weight of purified water. A cosmetic composition for skin whitening was prepared by mixing.

<Example 2> Preparation of cosmetic composition for skin whitening-2

A cosmetic composition for skin whitening was prepared by adding 2 parts by weight of the fermented extract prepared in Preparation Example 3 to the cosmetic composition for skin whitening of Example 1.

<Experimental Example 1> Analysis of active ingredient content

In order to analyze the polyphenol content and flavonoid content of the fermented extract prepared in Preparation Example 1-3, high-performance liquid chromatography (HPLC) analysis was performed, and the total polyphenol content and total flavonoid content were analyzed, and the results were It is shown in Table 1 below.

Total polyphenol content
(mg/g) Total flavonoid content
(mg/g) Manufacturing Example 1 97.32 20.31 Production example 2 138.57 28.93 Production example 3 203.47 32.56

As shown in Table 1, the fermented extract according to the present invention was confirmed to have high total polyphenol content and high total flavonoid content.

<Experimental Example 2> Anti-inflammatory and antioxidant effect analysis

In order to confirm the anti-inflammatory and antioxidant effects of the composition prepared in Example 1-2, an experiment was performed to determine whether the production of nitric oxide could be suppressed and an experiment to confirm DPPH free radical scavenging activity. and the results are shown in Table 2 below.

Efficacy (%) Example 1 Example 2 Anti-inflammatory (NO) 66.0 73.7 Antioxidant (DPPH) 72.3 79.4

As shown in Table 2, it was confirmed that the composition according to the present invention exhibits anti-inflammatory and antioxidant effects, and thus has an excellent skin improvement effect.

<Experimental Example 3> Analysis of skin whitening effect

1. Confirmation of melanin production inhibition effect

In order to confirm the whitening effect of the compositions of Examples 1 and 2, it was confirmed whether they inhibited the production of synthesized melanin. Specifically, B16F1 cells were dispensed into a 6 well plate and cultured for 24 hours under cell culture conditions. Afterwards, the cells were treated with 1% of each composition as the control and test substances, and for melanin synthesis, α-MSH was added to a final concentration of 10 nM and further cultured for 24 hours. At this time, four types of single substances included in the composition, including 10% Streptococcus thermophilus ferment, 30 mg/L chlorogenic acid, 10 mg/L ferulic acid, and 10 mg/L resveratrol, were also treated at 1% each. Then, the culture medium was removed, washed three times with PBS, and 300 μL of 1 N NaOH solution was added to lyse the cells at 90°C for 1 hour. The amount of melanin in the cells was determined by measuring the absorbance at 492 nm, and the results are shown in Table 3.

α-MSH Melanin amount (%) control group - 24.40 + 100.00 Example 1 + 69.46 Example 2 + 73.86

As shown in Table 2, it was confirmed that the whitening effect was excellent by suppressing melanin production in cells treated with the composition according to the present invention compared to the control group.

2. Check the whitening effect

In order to confirm the whitening effect of the composition prepared in Examples 1 and 2, 50 healthy women aged 30 to 40 were randomly divided into 3 groups and administered the compositions of Examples 1 and 2 every morning and evening. After washing the face several times, an appropriate amount was applied to the left and right sides of the face continuously for 2 months.

The skin whitening effect was evaluated by observing the degree of facial improvement one month after the start of the experiment and after the end of the experiment, and measuring the number of test subjects who showed the effect. The experimental results are shown in Table 4 below.

When to observe division available
(number of people) slightly valid
(number of people) invalidity
(number of people) effective rate
(%) 1 month later Example 1 37 8 5 90.0 Example 2 41 7 2 98.0 After the experiment ends Example 1 39 8 3 94.0 Example 2 44 6 0 100.0

As shown in Table 4, it was confirmed that the composition according to the present invention had excellent whitening effect.

Claims (3)

2-4 parts by weight of onion extract, 1-3 parts by weight of fermented extract, 0.6-0.8 parts by weight of polyglyceryl-6 behenate, 0.4-0.6 parts by weight of hydrogenated poly (C6-14 olefin), 2-4 parts by weight of glycerin parts, panthenol 0.7-0.9 parts by weight, dipropylene glycol 0.4-0.6 parts by weight, caprylic/capric triglyceride 0.4-0.6 parts by weight, t-butyl alcohol 1.3-1.5 parts by weight, niacinamide 2-4 Parts by weight, Hydrogenated polydecene 0.04-0.06 parts by weight, Polyacrylate crosspolymer-6 1.2-1.4 parts by weight, 1,2-hexanediol 0.3-0.5 parts by weight, Disodium EDTA 0.04-0.06 parts by weight , glyceryl acrylate/acrylic acid copolymer 1.7-1.9 parts by weight, sodium chloride 0.04-0.06 parts by weight, glyceryl stearate 0.6-0.8 parts by weight, sodium stearoyl glutamate 0.5-0.7 parts by weight, ethylhexylglycerin 0.5- 0.7 parts by weight, damask rose callus extracellular vesicles 0.6-0.8 parts by weight, allantoin 1-3 parts by weight, dipotassium glycyrrhizate 0.04-0.06 parts by weight, butylene glycol 1-3 parts by weight, hydrogenated lecithin 1-3 parts by weight, polyglyceryl-10 stearate 0.3-0.5 parts by weight, beta-glucan 0.8-1.0 parts by weight, sodium ascorbyl phosphate 0.04-0.06 parts by weight, tocopherol 1-3 parts by weight, tranexamic acid 0.4 -0.6 parts by weight, glutathione 0.4-0.6 parts by weight, alpha-bisabolol 0.4-0.6 parts by weight, alpha-arbutin 0.4-0.6 parts by weight, 3-O-ethyl ascorbic acid 0.4-0.6 parts by weight, pyridoxine 0.4-0.6 Parts by weight, Folic acid 0.04-0.06 parts by weight, Biotin 0.4-0.6 parts by weight, Pentylene glycol 0.4-0.6 parts by weight, Cyanocobalamin 0.04-0.06 parts by weight, Xanthan gum 1-3 parts by weight, Guar gum 1- 3 parts by weight, fructooligosaccharide 0.4-0.6 parts by weight, phytic acid 0.04-0.06 parts by weight, glucose 0.04-0.06 parts by weight, hydrolyzed pea protein 0.4-0.6 parts by weight, thiamine HCL 0.04-0.06 parts by weight parts by weight, riboflavin 0.7-0.9 parts by weight, linoleic acid 0.3-0.5 parts by weight, beta-carotene 1.5-1.7 parts by weight, bioflavonoids 0.04-0.06 parts by weight, sodium succinate 0.2-0.4 parts by weight, glycoprotein 0.2- Containing 0.4 parts by weight and 57.74-57.76 parts by weight of purified water,
The fermented extract is,
Washing mulberry roots, pomegranate peels, and rhubarb stems and drying them at a temperature of 48-52°C for 5-7 hours; Heat treating dried mulberry roots, pomegranate peels, and rhubarb stems at a temperature of 118-122°C for 18-22 minutes; Grinding heat-treated mulberry roots, pomegranate peels, and red tree stems and mixing them at a weight ratio of 1:1:1 to prepare a mixed powder; Mixing 48-52 parts by weight of the mixed powder and 48-52 parts by weight of the bisuri extract, adding 0.3-0.7 parts by weight of cellulase, and performing an enzyme reaction at a temperature of 32-36°C for 2-4 days; After the enzyme reaction, heating to a temperature of 78-82°C and cooling to a temperature of 18-22°C; After cooling, adding 0.8-1.2 parts by weight of a strain containing Saccharomyces coreanus and fermenting at a temperature of 28-32°C for 2-4 days; After fermentation, adding 38-42 parts by weight of purified water and extracting at a temperature of 118-122°C for 8-10 hours to prepare an extract; and concentrating and freeze-drying the extract under reduced pressure.
The bisuri extract is,
Washing the raincoat and drying it at a temperature of 48-52°C for 5-7 hours; heat treating the dried non-suri at a temperature of 118-122°C for 18-22 minutes; Mixing 8-12 parts by weight of pulverized bisuri powder and 88-92 parts by weight of purified water, heating to a temperature of 98-102°C and boiling for 22-26 hours; A cosmetic composition for skin whitening, characterized in that it is prepared by performing the following steps: boiling and then filtering to obtain a filtrate. delete delete