KR102659715B1 - Cosmetic composition for promoting hair growth containing natural ingredients and menufacturing method thereof - Google Patents

Abstract

The purpose of the present invention is to provide a novel composition having a hair growth promoting effect. The present invention relates to a fermented product obtained by inoculating a golden silkworm cocoon with cauliflower mycelium; A hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; It provides a cosmetic composition for promoting hair growth comprising a methylline chloride fraction from P. prickly pear flower.

Description

Cosmetic composition for promoting hair growth containing natural ingredients and method for producing the same {COSMETIC COMPOSITION FOR PROMOTING HAIR GROWTH CONTAINING NATURAL INGREDIENTS AND MENUFACTURING METHOD THEREOF}

The present invention relates to a cosmetic composition for promoting hair growth containing natural ingredients and a method for producing the same.

Hair plays a variety of roles in the human body, including protecting the head, influencing external appearance, and maintaining the temperature of the head. Although it is not an important organ for maintaining life, it is an important part of the body that is a measure of health status and determines appearance.

There are about 100,000 to 150,000 hairs in the human body, and each hair has a different cycle: an anagen, growing phase, which accounts for 90% of normal hair, a catagen, transitional phase, when growth stops and hair roots atrophy, Hair continues to grow and fall out as it goes through the hair cycle, including the telogen (resting) phase, when the hair bulb dries and becomes club hair. This cycle is repeated over 3 to 6 years, and as a result, an average of 50 to 100 hairs are shed per day.

Alopecia refers to a phenomenon in the hair growth cycle where the proportion of hair in the anagen phase becomes shorter and the amount of hair in the catagen or telogen phase increases, resulting in abnormally increased hair shedding and hair that was growing normally falling out due to some cause. In most cases of hair loss that is not due to stress or disease, the hair naturally becomes thinner, weaker, and falls out as it loses nutrients. As this cycle accelerates, hair loss occurs in which the hair does not regenerate. Hair loss is known to occur due to unbalanced diet, excessive drinking and smoking, drug use, lack of sleep, stress, childbirth, environmental pollution, genetics, frequent perm and dyeing, and hormonal imbalance.

Treatment methods for hair loss include topical application, oral medication, health supplements, and hair transplant surgery, but these methods have a limited range of use and do not provide continuous hair loss relief. Additionally, the effects of drug treatment are temporary and may actually accelerate the production of scalp or sebum.

To treat hair loss, the approach is to increase hair follicle cells in the growth phase (Anagen) and reduce cells in the catagen and telogen phases. It activates Wnt/β-catenin, which decreases during the hair loss process, and increases hair growth factors IGF-1 and VEGF, thereby differentiating cells involved in hair formation.

Currently, the most well-known hair growth promoters, such as minoxidil (transdermal application) and finasteride (oral administration), have received FDA approval and are used clinically. In particular, minoxidil was first introduced as a vasodilator in the early 1970s, and it was discovered that hair growth was a side effect other than the drug effect, so it is currently used as a hair growth promoter. Finasteride is a hair loss inhibitor with a 5α-reductase inhibitory effect that originated from the development of a treatment for benign prostatic hyperplasia. It is being used. However, adverse drug reactions from currently used hair growth agents have been reported, which poses significant limitations to active treatment.

Accordingly, hair loss alleviation and hair growth promotion compositions using natural ingredients are being studied to minimize side effects. For example, Patent No. 10-1196523 discloses a natural product fermentation extract in which pine needles, seaweed, soybeans, and Japanese pepper are mixed and extracted, and then the extract is inoculated with lactic acid bacteria of the genus Lactobacillus. Republic of Korea Patent Publication No. 10-2011-0056156 discloses a hair growth composition containing rice bran and perilla pod extract as active ingredients and a method for manufacturing the same. Republic of Korea Patent Publication No. 10-2007-0107329 discloses a herbal composition for promoting hair growth, which is used by pulverizing pine needles, hawthorn, angelica root, black sesame seed, raw rehmannia root, arborvitae leaves, mature rehmannia root, black bean, green tea, kelp, seaweed, spinach, etc. The manufacturing method is disclosed.

Although hair growth promoting compositions using various natural ingredients have been studied, research on compositions that still have excellent hair growth promoting effects and using new natural ingredients is needed.

Republic of Korea Patent Publication No. 10-1196523

Republic of Korea Patent Publication No. 10-2011-0056156

Republic of Korea Patent Publication No. 10-2007-0107329

The purpose of the present invention is to provide a novel composition having a hair growth promoting effect in order to solve the above problems.

The present invention relates to a fermented product obtained by inoculating a golden silkworm cocoon with cauliflower mycelium; A hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; It provides a cosmetic composition for promoting hair growth comprising a methylline chloride fraction from P. prickly pear flower.

In one embodiment of the present invention, a fermented product obtained by inoculating a golden silkworm cocoon with cauliflower mycelium; A hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; Methyline chloride fraction from P. chinensis flowers; A mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheongung; and a cosmetic composition for promoting hair growth comprising a mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil, and jojoba oil.

In one embodiment of the present invention, 10 to 30 parts by weight of fermented product inoculated with cauliflower mycelium in a golden silkworm cocoon; 10 to 20 parts by weight of a hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; 10 to 30 parts by weight of methylline chloride fraction from P. chinensis flowers; 5 to 10 parts by weight of a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheongung; and 20 to 40 parts by weight of a mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil, and jojoba oil.

In one embodiment of the present invention, the composition may be a composition for preventing hair loss or promoting hair growth, characterized in that it is in the form of any one selected from hair tonic, hair shampoo, hair conditioner, hair essence, hair oil, and hair soap. there is.

In one embodiment of the present invention,

(I) The step of producing a fermented product inoculating the golden silkworm cocoon with cauliflower mycelium includes the steps of (1) adding purified water to the golden silkworm cocoon and sterilizing it; (2) inoculating cauliflower mycelium; (3) incubating at 25°C to 40°C for 10 to 15 days; and (II) a hydrolyzate obtained by inoculating Aspergillus oryzae, a yeast fungus, into the shredded golden cocoon and adding papain as a proteolytic enzyme. The manufacturing steps include (1) adding purified water to shredded golden cocoons and sterilizing them; (2) inoculating Aspergillus; (3) Incubating at 25°C to 40°C for 3 to 5 days, (III) preparing a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemums, dandelions and Cheongung (1) Adding purified water to black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheonggung and sterilizing them; and (2) mixing sterilized black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion, and Cheongung in a blender, and (IV) the fermented product prepared in (I) to (III) above, the hydrated product. A method for producing a cosmetic composition for promoting hair growth can be provided, comprising the step of stirring the decomposition product and the mixture and the mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil, and jojoba oil.

In one embodiment of the present invention, step (IV) includes 10 to 30 parts by weight of fermented product inoculated with cauliflower mycelium in a golden silkworm cocoon; 10 to 20 parts by weight of a hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; 10 to 30 parts by weight of methylline chloride fraction from P. chinensis flowers; 5 to 10 parts by weight of a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheongung; and a method of producing a cosmetic composition for promoting hair growth, which involves stirring 20 to 40 parts by weight of a mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil, and jojoba oil.

According to the cosmetic composition for promoting hair growth of the present invention, a fermented product obtained by inoculating a golden silkworm cocoon with cauliflower mycelium; Golden Cocoon Hydrolyzate; And by containing the methylline chloride fraction of P. chinensis flower, it provides a hair growth promoting effect and can be used as a raw material for various cosmetics and quasi-drugs.

The present invention also provides a fermentation product inoculating golden silkworm cocoons with cauliflower mycelium; Golden Cocoon Hydrolyzate; and a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemums, dandelions, and Cheongung along with methylline chloride fractions from P. chinensis flowers; And by containing a mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil, and jojoba oil, it provides a better hair growth promotion effect and can be used as a raw material for various cosmetics and quasi-drugs.

Figure 1 is a diagram showing the hair growth effect when the composition of the present invention is treated on a person.

The cosmetic composition for promoting hair growth according to the present invention is a hydrolyzate obtained by inoculating finely chopped golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; Fermented product of shredded golden silkworm cocoons inoculated with koji bacteria; and methylline chloride fraction from P. chinensis flowers.

The cosmetic composition for promoting hair growth according to the present invention further includes a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemums, dandelions and Cheonggung; and blended oils of castor oil, olive oil, sunflower seed oil, apricot kernel oil, and jojoba oil.

In this specification, the golden silkworm cocoon refers to a yellow cocoon built by "Golden Silk", a breed bred by the Rural Development Administration of the Republic of Korea by crossing Japanese breed Jam311 (JS169) and Chinese breed Jam312 (CS186) from 2005 to 2007. , it is known to contain more sericin than ordinary silkworm cocoons.

In this specification, cauliflower mycelium refers to the mycelium of cauliflower mushrooms, and cauliflower mushrooms are mushrooms of the calyx family of the Basidiomycete order Achyranthesia, which grow naturally in Korea, Japan, Europe, North America, and Australia, and have beta liver. It is known to contain large amounts.

In the present specification, the methylene chloride fraction from P. chinensis flowers is prepared by the following method. Water is added to the extract obtained by adding methanol as a solvent to P. p. tree flowers, and then methylene chloride, an organic solvent, is added and shaken to separate the organic solvent layer and the water layer.

Hereinafter, the configuration of the present invention will be described in detail through the following examples and test examples. However, the scope of the present invention is not limited to the following examples and experimental examples.

[Example 1] Preparation of a cosmetic composition for promoting hair growth

1. Add 10kg of purified water to 1kg of commercially purchased golden silkworm cocoon and stir at 37℃ for 3 hours, then sterilize the stirred mixture at 120~140℃ for 10~20 minutes. Afterwards, 300 g of cauliflower mycelium is inoculated into the sterilized golden cocoon and cultured in an incubator maintained at 30°C for 10 days.

2. Meanwhile, place 1 kg of commercially purchased golden silkworm cocoons in a blender and grind them to prepare shredded golden silkworm cocoons. 10kg of purified water was added to the shredded golden silkworm cocoon and stirred for 3 hours at 37°C. Then, 200g of Aspergillus Oryzae culture medium as a yeast fungus was added and fermented at 37°C for 2 days. Afterwards, 10 kg of papain, a proteolytic enzyme, was added to this fermentation mixture and hydrolyzed at 70°C for 1 day. Afterwards, the hydrolyzate was filtered using a 1 ㎛ filter to remove unreacted solids.

3. Meanwhile, the P. locust flowers were dried well, 300 g of powder was prepared, and then extracted for 3 hours while heating with methanol. After evaporating all the solvent and water, the extract was dried using a freeze dryer to obtain a powdered extract. In addition, water was added to the methanol extract, and then methylene chloride, an organic solvent, was added and shaken to separate the organic solvent layer and the water layer.

4. Meanwhile, add purified water to 500g each of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion, and Cheongung, and sterilize at 110~140℃ for 1~4 hours. Put sterilized black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion, and Cheonggung into a blender and mix by stirring.

5. After mixing 30 parts by weight of the fermented product prepared in 1. above, 20 parts by weight of the hydrolyzate prepared in 2., 20 parts by weight of the fraction prepared in 3., and 10 parts by weight of the mixture prepared in 4., castor oil , 20 parts by weight of a mixed oil of olive oil, sunflower seed oil, apricot seed oil, and jojoba oil was placed in a blender and stirred to mix, thereby preparing a cosmetic composition for promoting hair growth.

Examples 2 to 6 and Comparative Examples 1 to 4

The composition was prepared as in Example 1 above, but the component ratio was changed as follows. In the table below, the fermented product is a fermented product obtained by inoculating a golden silkworm cocoon with cauliflower mycelium, and the hydrolyzate is a hydrolyzate obtained by inoculating a shredded golden silkworm cocoon with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme. The fraction is a methylline chloride fraction from prickly pear blossom, the mixture is a mixture of black sesame, black bean, Cheoncho, sorrel, wild chrysanthemum, dandelion and Cheongung, and the mixed oil is castor oil, olive oil, sunflower seed oil, apricot seed oil and jojoba oil. refers to a mixture of oils. Additionally, the unit is parts by weight.

Test Example 1: Test for the effect of promoting hair papilla cell proliferation

The keratin protein that makes up hair is produced in keratinocytes at the hair root, and these keratinocytes are differentiated from dermal papilla cells. Therefore, by testing the effect of promoting proliferation of dermal papilla cells, the effect of promoting hair growth can be confirmed.

Rat Vibrissa dermal papillae cells; PVGDPLTt sa created by transfecting the polyomavirus large T gene encoding a temperature-sensitive T antigen into PVG rat vibrissa dermal papilla cells 6 (hereinafter referred to as DP cells)) were used to evaluate the effect of promoting dermal papilla cell proliferation of the composition according to the present invention.

First, DP cells were prepared in a 96-well plate at a concentration of approximately 10 ⁴ cells/well. The compositions of Comparative Examples and Examples of the present invention were dissolved in DMSO (Dimethyl sulfoxide) or ethanol, respectively, and then serially diluted with DMEM medium containing 5% FBS serum concentration to reach a final concentration of 10 ppm. As a control, soybean extract extracted with 20% ethanol was used. 200 ㎕ of medium containing the compositions of Comparative Examples and Examples of the present invention was added to each well, and then cultured in an incubator at 37°C and 5% CO2 for about 3 days. 50 μl of MTT solution prepared at a concentration of 2 mg/ml was added to each well, and then left in the same incubator for 4 hours. When it was determined that the MTT reagent was absorbed into the intracellular mitochondria, the supernatant was removed, 150 μl of DMSO was added, and the mixture was stirred for 10 minutes to uniformly dissolve the formazan dye. Then, the OD value was measured at a wavelength of 515 nm using an ELISA reader, and the number of cells was measured according to a previously prepared standard curve. Based on the measured cell number, the dermal papilla cell proliferation promoting ability (%) of the comparative examples and examples of the present invention was calculated, and the results are shown in Table 2 below.

Test Example 2: Animal model hair growth effect test

As experimental animals, 6-week-old male SD (Sprague Dawley) laboratory rats were purchased from Orient Bio (Sung-nam, Korea) and reared at a temperature of 20 ± 2°C, humidity of 55 ± 5%, and light and dark conditions for 12 hours. The experimental animals were acclimatized for one week after purchase and then classified into a total of 10 groups, with 2 animals in each group. After hair was removed on the backs of rats in each group, 1% testosterone (TXN) was applied for one week to suppress hair growth.

The compositions of Examples 1 to 6 and Comparative Examples 1 to 4 were applied to the backs of hair-removed laboratory rats once a day in an amount of 0.2 ml. In the positive control group, 5% minoxidil was applied in the same way, and in the control group, a certain amount of physiological saline was applied by spray to provide the same stress. The application was carried out over 5 weeks, and after the test, the hair length was measured to the nearest 0.01 mm with a vernier caliper (Digimatic Caliper 500-181-30, Mitutoyo, Japan).

The test results of the above test examples are shown in Table 3 below. As shown in the table below, the compositions of Examples 1 to 6 grew hair at a level similar to that of the positive control group, and the compositions of Comparative Examples 1 to 4 grew hair at a level similar to the control group.

Test Example 3: Hair growth effect test on humans

A clinical test was conducted using the composition of Example 1 and the compositions of Comparative Examples 1 to 4, which showed the best effect in Test Example 1.

For 20 male and female subjects experiencing hair loss, hair tonics containing the compositions of Example 1 and Comparative Examples 1 to 4 were prepared according to Preparation Example 1 below, and the scalp area was divided into three parts with similar hair thickness and density. Divide into each area and use 5ml once a day in the morning and once a day in the evening (twice a day). After using the hair tonic for 1 month and 2 months, the change in hair thickness (diameter) and hair density were measured using a phototrichogram. The change in hair thickness is calculated as the difference in thickness measured after using the hair tonic for 1 and 2 months based on the thickness measured before using the hair tonic, and hair density is calculated as the number of hairs per 1 cm ² .

Additionally, a photograph of the hair of a woman in her 20s who participated in a clinical trial using the composition of Example 1 is shown in Figure 1.

Below are examples of formulations for cosmetics or quasi-drugs containing the composition according to the present invention as an active ingredient.

<Formulation Example 1> Preparation of hair tonic

<Formulation Example 2> Preparation of hair shampoo

Claims (6)

Fermented product of golden silkworm cocoons inoculated with cauliflower mycelium;
A hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; and
Contains a methylline chloride fraction from P. prickly pear flowers,
A mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheongung; and
Further comprising a blended oil of castor oil, olive oil, sunflower seed oil, apricot seed oil and jojoba oil,
Cosmetic composition for promoting hair growth. delete According to paragraph 1,
10 to 30 parts by weight of fermented product inoculated with cauliflower mycelium on golden silkworm cocoons;
10 to 20 parts by weight of a hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme;
10 to 30 parts by weight of methylline chloride fraction from P. chinensis flowers;
5 to 10 parts by weight of a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheongung; and
A cosmetic composition for promoting hair growth comprising 20 to 40 parts by weight of a mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil, and jojoba oil. According to claim 1,
A cosmetic composition for promoting hair growth, characterized in that the composition is in the form of any one selected from hair tonic, hair shampoo, hair conditioner, hair essence, hair oil, and hair soap. A method for producing a cosmetic composition for promoting hair growth, comprising:
(I) The step of producing a fermented product inoculated with cauliflower mycelium in a golden silkworm cocoon is:
(1) Adding purified water to the golden cocoon and sterilizing it;
(2) inoculating cauliflower mycelium;
(3) culturing at 25°C to 40°C for 10 to 15 days,
(II) The step of preparing the hydrolyzate obtained by inoculating the shredded golden silkworm cocoon with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme,
(1) Adding purified water to shredded golden cocoons and sterilizing them;
(2) inoculating Aspergillus;
(3) Including culturing at 25℃~40℃ for 3~5 days,
(III) The step of manufacturing a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheongung,
(1) Adding purified water to black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheonggung and sterilizing them; and
(2) Putting sterilized black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion, and Cheongung into a blender and mixing them,
(IV) hair growth comprising the step of stirring the fermented product, hydrolyzate and mixture prepared in (I) to (III) above and mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil and jojoba oil. Method for producing a cosmetic composition for stimulation. According to claim 5,
The step (IV) is,
10 to 30 parts by weight of fermented product inoculated with cauliflower mycelium on golden silkworm cocoons; 10 to 20 parts by weight of a hydrolyzate obtained by inoculating shredded golden silkworm cocoons with Aspergillus oryzae, a yeast fungus, and adding papain as a proteolytic enzyme; 10 to 30 parts by weight of methylline chloride fraction from P. chinensis flowers; 5 to 10 parts by weight of a mixture of black sesame seeds, black beans, Cheoncho, Sophora ginseng, wild chrysanthemum, dandelion and Cheongung; and a method for producing a cosmetic composition for promoting hair growth, comprising stirring 20 to 40 parts by weight of a mixed oil of castor oil, olive oil, sunflower seed oil, apricot seed oil, and jojoba oil.