KR102573677B1 - Composition for preventing, improving or treating inflammatory disease comprising the Pediococcus inopinatus WiKim0139 - Google Patents

Abstract

The present invention relates to a novel Pediococcus inopinatus WiKim0139 isolated from kimchi and a composition containing the same. Since Pediococcus inophinatus WiKim0139 according to the present invention exhibits an inhibitory effect on inflammatory cytokines, it can be variously used for prevention, improvement, and treatment of inflammatory diseases.

Description

Composition for preventing, improving or treating inflammatory disease comprising the Pediococcus inopinatus WiKim0139 {Composition for preventing, improving or treating inflammatory disease comprising the Pediococcus inopinatus WiKim0139}

The present invention relates to a novel Pediococcus inophinatus strain isolated from kimchi and a composition for preventing, improving or treating inflammatory diseases including the same.

Inflammation is a local or systemic defense mechanism against cell and tissue damage or infection. Inflammation is a direct response of humoral mediators that make up the immune system or by stimulating local or systemic effector systems. It is caused by a chain of biological reactions that occur. Major inflammatory diseases include digestive diseases such as gastritis and inflammatory enteritis, oral diseases such as periodontitis, respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and rhinitis, skin diseases such as atopic dermatitis, hair loss, and psoriasis, degenerative arthritis, arthritis such as rheumatoid arthritis; and metabolic diseases such as obesity, diabetes, and liver cirrhosis (Patent Document 0001).

Inflammation mediators expressed at inflammatory sites, that is, cytokines such as IL-1α, IL-1β, IL-6, IL-8, chemokines, reactive oxygen species, cyclooxygenase-2 ( Cycloxygenase-2, COX-2), 5-lipoxygenase (5-LOX), and matrix metalloproteinase (MMP) play an important role in the generation and maintenance of inflammatory responses. The expression of these inflammatory mediators is regulated by the transcription factors NF-κβ (nuclear factor κβ), STAT3 (signal transducer and activator of transcription 3), AP-1 (activator protein 1), HIF-1a (hypoxiainducible factor 1a), etc. It is known (Patent Document 0001).

Psoriasis, one of the inflammatory diseases, is a chronic inflammatory skin disease that occurs in skin tissues due to an excessive increase in immune response of abnormally activated T cells and is one of the intractable immune diseases to be overcome with autoimmune diseases. In modern society, the number of patients with psoriasis is increasing due to factors such as stress, climate change, and irregular life patterns, and when psoriasis develops, most of them develop into long-term diseases and may be accompanied by various diseases such as psoriatic arthritis, metabolic syndrome, and cardiovascular disease. can Psoriasis patients account for about 2-3% of the world's population, and in Korea, about 8,500 people have increased in the five years since 2012 to 168,000 people, which is about 3% of the population.

Psoriasis is a disease that can occur mainly in the 20s and last for 20 years. Due to the symptoms of external changes in which the skin cracks like a tortoise and peels off dead skin cells, the quality of life of psoriasis patients deteriorates and social problems are caused. Not only is the satisfaction level low, but as it is a chronic disease that requires lifelong treatment and management, medical expenses are greatly burdened. Therefore, it is desired to develop a technology for improving psoriasis that has fewer side effects, is effective in relieving inflammatory symptoms, is easy to take, and is safe.

Korean Patent Publication No. 10-2021-0031301

The present invention is to provide a novel lactic acid bacteria of the genus Pediococcus inopinatus having excellent efficacy in preventing or improving inflammatory diseases.

Accordingly, the present inventors have tried to find a lactic acid bacteria strain showing the effect of preventing or improving inflammatory diseases from traditional fermented foods, and as a result, a novel Pediococcus lactic acid bacteria strain, Pediococcus inopinatus WiKim0139 , was isolated and identified. Thus, the present invention was completed.

The present invention provides Pediococcus inopinatus WiKim0139 ( Pediococcus inopinatus WiKim0139 ).

Pediococcus inopinatus WiKim0139 of the present invention is a new strain of Pediococcus inopinatus derived from kimchi. Although Pediococcus inophinatus WiKim0139 in the present invention was isolated and identified from kimchi, its acquisition route is not limited thereto.

The lactic acid bacteria strain isolated through the examples of the present invention was found to have a nucleic acid sequence of SEQ ID NO: 1 as a result of 16S rDNA sequencing analysis for identification and classification of microorganisms.

Therefore, the microorganism of the present invention having the 16S rDNA sequence of SEQ ID NO: 1 was named Pediococcus inopinatus WiKim0139 ( Pediococcus inopinatus WiKim0139 ) and deposited with the Korea Microorganism Conservation Center on August 26, 2021 ( Accession No. KCCM13039P).

Pediococcus inophinatus WiKim0139 of the present invention is a gram-positive bacterium and is a facultative anaerobe capable of growing in both aerobic and anaerobic conditions, and does not form spores, has no motility, and cells take the form of bacilli. .

In the following examples, Pediococcus inophinatus WiKim0139 of the present invention increased inflammatory cytokines (IL-1βTNF-α, IL-6) and Th17 cytokines (IL-17) in back skin tissues when psoriasis was induced compared to the normal group. ), and when kimchi lactobacillus was administered, it was confirmed that the expression of inflammatory cytokines (IL-1βTNF-α, IL-6) and Th17 cytokines was suppressed compared to the control group. In addition, compared to the psoriasis-induced group, the Kimchi Lactobacillus-administered group suppressed the lymph node T cell IL-17 production, while the immune regulatory cytokine IL-10 production increased and the distribution of immune regulatory T cells increased. As a result, it was found that The preventive or ameliorative effect of the Pediococcus inopinatus WiKim0139 strain on psoriasis was confirmed. Therefore, Pediococcus inophinatus WiKim0139 according to the present invention can be variously used for preventing, treating, or improving human or animal psoriasis.

In addition, as can be seen in the following examples, the composition containing Pediococcus inophinatus WiKim0139 as an active ingredient inhibits cytokine (IL-1β, TNF-α, IL-6) and Th17 cytokine expression, IL It has the effect of inhibiting -17 production and increasing IL-10 production, and the composition can be used as an active ingredient of a composition for preventing, treating or improving inflammatory diseases.

The present invention provides a composition for preventing, improving or treating inflammatory diseases comprising Pediococcus inophinatus WiKim0139, a culture thereof, a lysate thereof, or an extract thereof as an active ingredient.

Pediococcus inophinatus WiKim0139 included in the composition according to the present invention may exist as live or dead cells, and may also exist in a dried or lyophilized form. Types of lactic acid bacteria suitable for inclusion in various compositions and formulation methods are well known to those skilled in the art. For example, Pediococcus inophinatus WiKim0139 is a culture obtained by culturing in a known liquid medium or solid medium, a fermented product obtained by culturing the strain and additional components together, or using the strain in an organic solvent. It may be formulated in the form of an extracted extract, a cell membrane of the strain dissolved, or a lysate (or lysate) obtained by crushing or homogenizing, but is not limited thereto.

In one embodiment, the composition may be a composition containing the Pediococcus inophinatus WiKim0139 strain, which exists as live or dead bacteria.

In the present invention, the inflammatory disease is gastritis, stomatitis, atopic dermatitis, acne, psoriasis, sinusitis, rhinitis, conjunctivitis, asthma, dermatitis, inflammatory collagen vascular disease, glomerulonephritis, encephalitis, inflammatory enteritis, chronic obstructive pulmonary disease, sepsis, Septic shock, pulmonary fibrosis, undifferentiated spondyloarthrosis, undifferentiated arthropathy, arthritis, inflammatory osteolysis, chronic inflammatory diseases caused by viral or bacterial infections, ulcerative colitis, inflammatory bowel disease, rheumatoid arthritis, reactive arthritis, osteoarthritis, scleroderma , osteoporosis, atherosclerosis, myocarditis, endocarditis, pericarditis, cystic fibrosis, Hashimoto's thyroiditis, Graves' disease, leprosy, syphilis, Lyme disease, Borreliosis, neurogenic-borreliosis, tuberculosis, sarcoidosis ( sarcoidosis), lupus, lupus lupus, lupus tuberculosis, lupus nephritis, systemic lupus erythematosus, macular degeneration, uveitis, irritable bowel syndrome, Crohn's disease, Sjogren's syndrome, fibromyalgia, chronic fatigue syndrome, chronic fatigue immunodeficiency syndrome, myalgic encephalomyelitis , amyotrophic lateral sclerosis, Parkinson's disease, and multiple sclerosis, preferably psoriasis.

In one embodiment, the composition may be a pharmaceutical composition for preventing or treating inflammatory diseases comprising a culture, lysate, fermented product or extract of Pediococcus inophinatus WiKim0139 strain.

When the composition according to the present invention is used as a pharmaceutical composition, the pharmaceutical composition of the present invention can be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the Pediococcus inophinatus WiKim0139 strain, As the adjuvant, excipients, disintegrants, sweeteners, binders, coating agents, expanding agents, lubricants, lubricants, or flavoring agents may be used.

The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration.

For example, for formulation in the form of a tablet or capsule, the active ingredient may be combined with an oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, or water. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate. ate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methylcellulose, agar, bentonite, xanthan gum, and the like. For compositions formulated as liquid solutions, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added if necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to prepare formulations for injections such as aqueous solutions, suspensions, and emulsions, pills, capsules, granules, or tablets.

Furthermore, it can be preferably formulated according to each disease or component using a method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

In one embodiment, the present invention provides a food composition for preventing or improving inflammatory diseases comprising Pediococcus inophinatus WiKim0139, a culture thereof, a lysate thereof, a fermented product thereof, or an extract thereof as an active ingredient. The food composition may include a form of health functional food or beverage, bar, or the like.

In the present invention, the food composition containing the strain as an active ingredient may include a beverage such as fermented milk.

The food composition according to the present invention can be formulated in the same way as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, vitamin complexes, health supplements, and the like.

The food composition of the present invention may include ingredients commonly added during food preparation, and include, for example, proteins, carbohydrates, fats, nutrients, seasonings, and flavoring agents. Examples of the aforementioned carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides such as conventional sugars such as dextrins and cyclodextrins and sugar alcohols such as xylitol, sorbitol and erythritol. As flavoring agents, natural flavoring agents [thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used. For example, when the food composition of the present invention is made into drinks and beverages, citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be further included.

The composition according to the present invention may be used as a feed additive or feed.

When used as a feed additive, the composition may be at a high concentration of 20 to 90% or prepared in powder or granular form. The feed additives are organic acids such as citric acid, fumaric acid, adipic acid, lactic acid, and malic acid, sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate (polyphosphate), polyphenol, catechin, alpha-tocopherol, and rosemary. extract, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, phytic acid, and the like, or any one or more natural antioxidants may be further included. When used as a feed, the composition may be formulated in a conventional feed form, and may include a common feed component together.

The feed additives and feeds include grains such as pulverized or crushed wheat, oats, barley, corn and rice; vegetable protein feeds such as those based on rape, soybean, and sunflower; animal protein feed such as blood meal, meat meal, bone meal and fish meal; It may further include sugar and dairy products, for example, dry ingredients composed of various powdered milk and whey powder, etc., and may further include nutritional supplements, digestion and absorption enhancers, growth promoters, and the like.

The feed additive may be administered to animals alone or in combination with other feed additives in an edible carrier. In addition, the feed additives can be easily administered to animals as a top dressing, directly mixed with animal feed, or in an oral formulation separate from feed. When the feed additive is administered separately from animal feed, it can be prepared as an immediate release or sustained release formulation by combining it with a pharmaceutically acceptable edible carrier, as is well known in the art. Such edible carriers can be solid or liquid, for example corn starch, lactose, sucrose, soybean flakes, peanut oil, olive oil, sesame oil and propylene glycol. When a solid carrier is used, the feed additive may be a tablet, capsule, powder, troche or sugar-containing tablet or top dressing in a microdispersible form. When a liquid carrier is used, the feed additive may be a gelatin soft capsule, or a syrup, suspension, emulsion, or solution formulation.

In addition, the feed additives and feeds may contain auxiliary agents, for example, preservatives, stabilizers, wetting agents or emulsifying agents, solution accelerators, and the like. The feed additive may be used by adding it to the animal's feed by soaking, spraying or mixing.

The feed or feed additive of the present invention can be applied to a number of animal diets including mammals, poultry and fish.

In the present invention, animals include mammals, and as mammals, pigs, cows, sheep, goats, laboratory rodents, and laboratory rodents, as well as pets (eg, dogs and cats) may be used, but are not limited thereto. no.

The amount of Pediococcus inophinatus WiKim0139 strain included in the composition according to the present invention may be about 10 ⁶ to 10 ¹² cfu / g, for example, 10 ⁷ to 10 ¹¹ cfu / g, 10 ⁸ to 10 ¹⁰ cfu/g. When administering a strain, it is preferable to administer it in a viable state, and it can be killed before ingestion or administered in an attenuation state. In addition, when manufacturing using a culture supernatant, etc., a sterilization process through a heat treatment process may be additionally performed. The amount of strain and daily intake required to have the minimum efficacy may vary depending on the body or health condition of the consumer, but may generally be about 10 ⁶ to 10 ¹² cfu/g, such as 10 ⁷ to 10 ¹¹ cfu/g. , 10 ⁸ to 10 ¹⁰ cfu/g.

The advantages and features of the present invention, and how to achieve them, will become clear with reference to the detailed description of the following embodiments. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various different forms, and only the present embodiments will complete the disclosure of the present invention and allow common knowledge in the art to which the present invention belongs. It is provided to fully inform the holder of the scope of the invention, and the present invention is only defined by the scope of the claims.

Pediococcus inophinatus WiKim0139 according to the present invention is a lactic acid bacterium isolated from kimchi and exhibits an excellent inflammatory cytokine inhibitory effect, so it can be used in various ways for the prevention, improvement, and treatment of inflammatory diseases.

Figure 1 is a picture (a) and a graph (b) of the selection step of kimchi lactobacillus candidate strains having disease amelioration using antigen-specific T cells.
Figure 2 is a picture showing an experimental method for confirming the effect of psoriasis mice through application of Imiquimod cream.
Figure 3 is a photograph (a) and ear thickness measurement graph (b) confirming the improvement of psoriasis symptoms according to the intake of kimchi lactic acid bacteria WiKim0139.
4 is a graph showing psoriasis improvement efficacy through confirmation of inflammatory marker mRNA expression in tissues such as psoriasis mice.
Figure 5 is a psoriasis improvement efficacy verification graph through the confirmation of T cell cytokine expression in the lymph node.
6 is a graph showing distribution of immunoregulatory T cells in lymph nodes.

Hereinafter, the present invention will be described in detail through examples. The following examples are merely illustrative of the present invention, but the scope of the present invention is not limited to the following examples.

[Example]

Example 1: Isolation and cultivation of Pediococcus inophinatus WiKim0139

The present inventors isolated kimchi lactic acid bacteria from kimchi. After crushing the kimchi sample and spreading the stock solution of the kimchi extract on MRS agar medium (Difco), it was incubated at 30 ° C for more than 24 hours. Single colonies formed after culturing were subcultured to select uniform colonies, and the selected uniform colonies were finally identified through 16S RNA sequencing.

A single colony of Pediococcus inopinatus cultured on MRS agar medium (Difco) was cultured in MRS medium at 30 ° C. for 24 hours, and then the cells were centrifuged at 8,000 rpm for 5 minutes and then rinsed with PBS. The remaining medium components were removed. .

As a result of 16S rDNA sequencing analysis for identification of microorganisms, the strain isolated through the examples of the present invention was found to have a nucleic acid sequence of SEQ ID NO: 1.

Accordingly, the microorganism of the present invention was named Pediococcus inopinatus WiKim0139 ( Pediococcus inopinatus WiKim0139) and was deposited with the Korea Microorganism Conservation Center on August 26, 2021 (Accession No. KCCM13039P).

Pediococcus inophinatus WiKim0139's API 50 CHL kit (Biomerieux) analysis results are shown in Table 1 below.

test number reactive substrate WiKim0139 test number reactive substrate WiKim0139 24h 48h 24h 48h 0 control - - 25 Esculin + + One glycerol - - 26 Salicin - v 2 erythritol - - 27 cellobiose + + 3 D-arabinose - - 28 maltose v + 4 L-arabinose - - 29 lactose - - 5 Ribose - - 30 melibios - - 6 D-xylose - - 31 sucrose - - 7 L-Xylose - - 32 trehalose v + 8 adonitol - - 33 inulin - - 9 ß methyl-D-xyloside - - 34 melezitus - - 10 galactose + + 35 Raffinose - - 11 glucose + + 36 starch - - 12 fructose + + 37 glycogen - - 13 mannose + + 38 xylitol - - 14 sorbos - - 39 ß gentiobios + + 15 Rhamnos - - 40 D-Turanos - - 16 dulcitol - - 41 D-lysose - - 17 inositol - - 42 D-tagatose - - 18 mannitol - - 43 D-Fucose - - 19 sorbitol - - 44 L-fucose - - 20 Methyl-D-Mannoside - - 45 D-arabitol - - 21 Methyl-D-glucoside - - 46 L-arabitol - - 22 N-acetyl-glucosamine + + 47 gluconate - - 23 amygdalin - v 48 2-keto-gluconate - - 24 Arbutin - - 49 5-keto-gluconate - -

Example 2: Results of confirming the psoriasis improvement effect of Pediococcus inophinatus WiKim0139

Candidate strains of kimchi lactic acid bacteria having disease amelioration using antigen-specific T cells were selected by the method of FIG. 1 . Pediococcus inophinatus WiKim0139 (W0139), which has a higher immunomodulatory cytokine production ratio (IL-10/IL-17) than the cognate and cognate Pediococcus inophinatus PI01 strain, was selected (FIG. 1 (b) ).

Pediococcus inopinatus WiKim0139 was orally administered to psoriasis mice coated with Imiquimod cream for 3 weeks (FIG. 2), and improvement in psoriasis was confirmed using a clinical visual evaluation method (FIG. 3). Imiquimod cream was applied to the skin of the ears and back (psoriasis-induced control group), and the group ingesting Dexa, a psoriasis treatment, and the group ingesting WiKim0139 were compared for 3 weeks (Fig. 3). As a result, the normal group (naive) In comparison, in the psoriasis-induced control group, erythema and swelling were confirmed in the ear and back tissues, which are the standard for sensory evaluation, and in the group ingested WiKim0139, erythema and swelling were alleviated, and it was noticeable on the skin of the back. Significant improvement in psoriasis symptoms was observed (Fig. 3 (a)), and it was confirmed that glans thickness was reduced compared to the control group (Fig. 3 (b)).

Example 3: Inflammatory cytokine test results of Pediococcus inophinatus WiKim0139

In Example 2, the expression of inflammatory marker mRNA in skin tissues such as psoriasis mice was confirmed (FIG. 4). It can be confirmed that the expression of inflammatory cytokines (IL-1βTNF-α, IL-6) and Th17 cytokines (IL-17) is increased in the back skin tissue of the psoriasis-induced control group (IMQ) compared to the normal group (Naive). there was, In the back tissues of mice ingested WiKim0139, the expression of IL-1β, TNF-α, IL-6, IL-17, IL-22 and IL-23 was reduced.

Expression of T-cell cytokines in lymph nodes of psoriasis mice was also confirmed. (FIG. 5). It was confirmed that IL-17 expression decreased and IL-10 expression increased in lymph node T cells of mice ingested WiKim0139.

Example 4: Test results for verifying the distribution of immunoregulatory T cells in the lymph node of Pediococcus inophinatus WiKim0139

In Example 2, the distribution of immunoregulatory T cells in lymph nodes of psoriasis mice was verified (FIG. 6). In the group ingesting WiKim0139, it was confirmed that the distribution of immunoregulatory T cells in lymph nodes increased.

Korea Microbial Conservation Center (Overseas) KCCM13039P 20210826

<110> KOREA FOOD RESEARCH INSTITUTE <120> Pediococcus inopinatus WiKim0139 <130> P21R10C0820 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1420 <212> DNA <213> unknown <220> 223 <Pediococcus inopinatus> <400> 1 acgcactttc gttgaatgat ttagagatgc ttgcatcgaa gatgatttta actataaagt 60 gagtggcgaa cgggtgagta acacgtgggt aacctgccca gaagtggggg ataaccctg 120 gaaacagatg ctaataccgc ataataaagt aaaccgcatg gttttctttt aaaagatggc 180 ttcggctatc acttctggat ggacccgcgg cgttatagct agttggtgag ataaaggccc 240 accaaggctg tgatacgtag ccgacctgag agggtaatcg gccacattgg gactgagaca 300 cggcccagac tcctacggga ggcagcagta gggaatcttc cacaatggac gaaagtctga 360 tggagcaacg ccgcgtgagt gatgaaggct ttagggtcgt aaaactctgt tgttggagaa 420 gaacgtgtgt gagagtaact gctcatgcag tgacggtatc caaccagaaa gccacggcta 480 actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttatccgga tttatgggc 540 gtaaagcgag cgcaggcggt ttcttaagtc taatgtgaaa gccttcggct taaccgaaga 600 agtgcattgg aaactgggaa acttgagtgc agaagaggac agtggaactc catgtgtagc 660 ggtgaaatgc gtagatatat ggaagaacac cagtggcgaa ggcggctgtc tggtctgtaa 720 ctgacgctga ggctcgaaag catgggtagc gaacaggatt agataccctg gtagtccatg 780 ccgtaaacga tgaatgctaa gtgttggagg gtttccgccc ttcagtgctg cagctaacgc 840 attaagcatt ccgcctgggg agtacgaccg caaggttgaa actcaaaaga attgacgggg 900 gcccgcacaa gcggtggagc atgtggttta attcgaagct acgcgaagaa ccttaccagg 960 tcttgacatc ttctgctaac ctaagagatt aggcgttccc ttcggggaca gaatgacagg 1020 tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg 1080 caacccctat tattagttgc cagcattaag ttgggcactc tagtgagact gccggtgata 1140 aaccggagga aggtggggac gacgtcaaat catcatgccc cttatgacct gggctacaca 1200 cgtgctacaa tggacggtac aacgagttgc gagaccgcga ggttaagcta atctcttaaa 1260 accgttctca gttcggactg caggctgcaa ctcgcctgca cgaagttgga atcgctagta 1320 atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac 1380 accatgagag tttgtaacac ccaaagccgg tggagtaacc 1420

Claims (11)

Pediococcus inopinatus WiKim0139 with accession number KCCM13039P { Pediococcus inopinatus WiKim0139 }. Pediococcus inopinatus WiKim0139 ( Pediococcus inopinatus WiKim0139 ; accession number KCCM13039P), a culture thereof, a lysate thereof, or a food composition for preventing or improving psoriasis containing an extract thereof as an active ingredient. delete According to claim 2,
The psoriasis is caused by the expression of one or more cytokines selected from the group consisting of IL-1β, TNF-α, IL-6, IL-17, IL-22 and IL-23, food composition. According to claim 2,
The food is a food composition that is a health functional food. According to claim 2,
A food composition wherein the food is a beverage or bar. Pediococcus inopinatus WiKim0139 (Pediococcus inopinatus WiKim0139 ; accession number KCCM13039P) or a lactic acid bacteria starter for fermentation comprising a culture thereof. Pediococcus inopinatus WiKim0139 ( Pediococcus inopinatus WiKim0139 ; accession number KCCM13039P), a feed or feed additive composition comprising a culture thereof, a lysate thereof, or an extract thereof as an active ingredient. Pediococcus inopinatus WiKim0139 ( Pediococcus inopinatus WiKim0139 ; accession number KCCM13039P), a pharmaceutical composition for preventing or treating psoriasis comprising a culture thereof, a lysate thereof or an extract thereof as an active ingredient. delete According to claim 9,
The psoriasis is caused by the expression of one or more cytokines selected from the group consisting of IL-1β, TNF-α, IL-6, IL-17, IL-22 and IL-23, the pharmaceutical composition.