KR102576404B1 - Sensor film, bio-sensor and apparatus comprising same - Google Patents

Abstract

The sensor film, which has a sensing material placed on the surface of a base film that can be wound in a roll, can be unwound at a constant speed and continuously supplied for reaction with the sample, so it can be used to supply sensor material, introduce samples, and conduct analysis. It is possible to implement a biosensor in which the entire process is automated.

Description

Sensor film, biosensor and device comprising the same {SENSOR FILM, BIO-SENSOR AND APPARATUS COMPRISING SAME}

The following embodiments relate to sensor films, biosensors and devices including the same. More specifically, embodiments relate to sensor films for detecting viruses, bacteria, mold, etc., and biosensors and devices including the same.

A biosensor refers to a device that investigates the properties of materials using the functions of living things. Since it uses biological materials such as blood sugar or ketones as a detection device, it has excellent sensitivity and response specificity. Therefore, biosensors are used in a wide range of fields, including clinical chemical analysis in the pharmaceutical field, process measurement in the bio industry, environmental measurement, and stability evaluation of chemical substances, and their scope continues to expand. For example, biosensors are widely used for various self-tests and rapid disease diagnosis, such as blood sugar measurement, pregnancy diagnosis, and urine tests, and are also used to measure viruses, bacteria, and mold in filters.

In particular, interest in health has increased significantly due to recent improvements in living standards, and research is actively underway to apply biosensors to everyday convenience devices or healthcare equipment. For example, applying biosensors to air conditioning and ventilation systems in buildings or vehicles to measure and manage bacteria in filters in real time, or applying biosensors to household products that come into contact with the body every day to automatically implement real-time health diagnosis. Attempts are being made.

Korean Patent Publication No. 2013-0032461

Conventional biosensors had difficulty applying them to various fields because the steps of collecting samples, introducing them into the biosensor, and analyzing them after a certain period of time had to be performed manually as most of them had not yet been automated.

In particular, since the substrate or test paper used in biosensors mainly uses biological binding such as a one-time antigen-antibody reaction, the need to manually replace the substrate or test paper for each analysis was the biggest obstacle to biosensor automation.

Accordingly, the present inventors derived the form of a continuous film as the best form for automatically supplying test strips, and in addition, focused on a roll-shaped continuous film as a form that could minimize the storage space of unused test strips.

Therefore, through the following embodiments, we intend to provide a sensor film designed to enable automated continuous supply using a roll-shaped film, a biosensor with an entire process automated, and a device for applying the same to various fields.

According to one embodiment, a base film that can be wound into a roll form; and a sensing material disposed on the surface of the base film.

According to another embodiment, the sensor film; first means for unwinding the sensor film; a second means for introducing a sample into the unwound sensor film; and a third means for analyzing a sample introduced into the sensor film.

According to another embodiment, the sensor film; a roll for unwinding the sensor film; filter; a tape that absorbs air passing through the filter and introduces a sample into the sensor film; and a measuring device for analyzing a sample introduced into the sensor film.

According to another embodiment, the sensor film; a roll for unwinding the sensor film; bidet; A collector connected to the bidet and collecting urine to introduce the sample into the sensor film; and a measuring device for analyzing a sample introduced into the sensor film.

According to another embodiment, the sensor film; a roll for unwinding the sensor film; urinal; a collector that collects urine flowing on the inner wall of the urinal and introduces the sample into the sensor film; and a measuring device for analyzing a sample introduced into the sensor film.

The sensor film according to the above embodiment is rollable, and after being wound, it can be unwound at a constant speed and continuously supplied for reaction with the sample. In addition, the sensor film can apply techniques such as well-known ELISA (enzyme-linked immunosorbent assay) and can be implemented using paper, fabric, or general flexible films.

When such a sensor film is applied to a biosensor, the problem of continuous supply of test strips, which was previously difficult to automate, can be solved. When connected, the sample introduction and analysis process can be automated with current technology, ultimately leading to the entire process. This automated biosensor can be implemented.

In particular, by controlling the unwinding speed of the sensor film in the biosensor, the sensing material can be induced to sufficiently react with the sample, and various detection analyzes can be performed simultaneously by arranging two or more types of sensing materials in parallel on the sensor film. can do.

Biosensors using such sensor films can be introduced into the air conditioning and ventilation systems of buildings or vehicles to manage bacteria in filters in real time, or introduced into bidets or urinals to diagnose health in real time by easily checking biometric information. It can be utilized.

Figure 1 is a schematic diagram of a sensor film according to one embodiment.
Figures 2a and 2b show the sensing area formed on the sensor film.
Figure 3 is a cross-sectional view of a sensing area in a sensor film according to one implementation.
Figure 4 shows a device equipped with a biosensor according to one embodiment.
Figure 5 shows a device equipped with a biosensor according to another embodiment.
Figure 6 shows a device equipped with a biosensor according to another embodiment.

In the case where each film or layer described in the following embodiments is described as being formed “on” or “under” each film or layer, the terms “on” and “under” are used. “(under)” includes everything that is formed directly or indirectly through other components.

Additionally, the size of each component in the drawings may be exaggerated for explanation and does not mean the actual size applied.

In addition, all numerical ranges representing the physical properties, dimensions, etc. of the components described in this specification should be understood as being modified by the term “about” in all cases unless otherwise specified.

[Sensor Film]

Figure 1 is a schematic diagram of a sensor film according to one embodiment. Referring to FIG. 1, the sensor film 100 according to one embodiment includes a base film 110 that can be wound in a roll shape; and a sensing material 120 disposed on the surface of the base film.

The sensor film according to the above embodiment may have a roll shape, and this roll shape is advantageous for storage and handling. Additionally, the sensor film may be unrolled from a roll for use and provided as a continuous film. On the other hand, existing biosensors used sensor boards or one-time test strips, making continuous analysis impossible.

In the roll-type sensor film, the radius of the bottom of the roll is not particularly limited, but may range from 50 mm to 300 mm or 100 mm to 200 mm, for example. In addition, the height of the roll in the roll-type sensor film is not particularly limited, but may range from 1 mm to 150 mm or 3 mm to 100 mm, for example.

In addition, the roll-shaped sensor film may have a radius of curvature in the range of 5R to 200R, or 20R to 100R, and as a result, it can be wound into a small roll, making it possible to miniaturize the device. The unit of radius of curvature (R) can be interpreted the same as the unit of length (mm). For example, 20R may mean the degree to which the circumference of a circle with a radius of 20 mm is curved.

In addition, as shown in FIG. 1, the roll-shaped sensor film 100 may be provided with a hole 130 in the central axis into which a paper tube is inserted, and the radius of the hole 130 is, for example, in the range of 5 mm to 50 mm, Or it may range from 5 mm to 20 mm.

base film

The base film serves as a medium for continuously supplying the sensing material. Accordingly, the base film is produced as a film that can be wound in a roll form. For example, the base film may have the form of a long continuous film.

The width of the base film is not particularly limited, but may, for example, range from 1 mm to 150 mm, or 3 mm to 100 mm. Additionally, the overall length of the base film is not particularly limited, but may be, for example, in the range of 0.1 m to 100 m, or 1 m to 10 m. Additionally, the thickness of the base film is not particularly limited, but may be, for example, in the range of 0.1 mm to 5 mm, or 0.3 mm to 3 mm.

The base film has flexibility so that it can be wound into a roll shape. For example, the base film may be bent 10 times or more, 50 times or more, 100 times or more, or 1000 times or more until cutting in an MIT bending test. At this time, the conditions for the MIT bending test may be a test piece of 1.5 cm x 10 cm, a load of 0.98 N, a bending clamp radius of curvature (R) of 2 mm, and a speed of 90 times/min.

The base film may be paper or a flexible polymer film. The flexible polymer film may include polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET), polycarbonate, polyvinyl chloride (PVC), polylactic acid (PLA), etc.

sensing material

The sensing material serves to detect a target object by contacting and reacting with the sample. For example, the sensing material may be used to detect viruses, bacteria, or mold. To this end, the sensing material may include an antibody for an antigen-antibody reaction.

As a specific example, the sensing material may include an antibody and a coloring agent for ELISA (enzyme-linked immunosorbent assay). The antibody and coloring agent may be appropriately selected from known antibodies and coloring agents depending on the target to be detected, and are not particularly limited.

The sensor film may include one type of sensing material for one detection target. Alternatively, the sensor film may include two or more types of sensing materials for different detection targets. In this case, the two or more types of sensing materials may be arranged in parallel along the width direction of the base film. Accordingly, analysis of multiple detection targets can be performed simultaneously.

Sensing area

The sensor film may be composed of a sensing area containing the sensing material and a non-sensing area excluding the sensing area.

Figures 2a and 2b show the sensing area formed on the sensor film.

Referring to FIG. 2A, the sensor film 100 includes a plurality of sensing regions 150 including the sensing material, and the plurality of sensing regions 150 are located along the longitudinal direction of the base film 110. It can be formed at regular intervals.

Alternatively, the sensor film may include two or more types of sensing materials for different detection targets. For example, referring to FIG. 2B, the plurality of sensing areas include a first sensing area 150' including a first sensing material for a first detection target, and a second sensing material for a second detection target. and a second sensing area 150'', wherein the first sensing area 150' and the second sensing area 150'' are arranged in parallel along the width direction of the base film 110. can be formed.

In addition, the plurality of sensing regions may further include a third sensing region including a third sensing material for an additional detection target, a fourth sensing region including a fourth sensing material, etc., which are It may be formed in parallel with the first sensing area and the second sensing area along the width direction.

Sensing area configuration example

The sensing area may have various detailed configurations depending on the technique used for detection. For example, when the sensor film uses ELISA technology, the sensing area can be configured to suit its operating principle.

Figure 3 is a cross-sectional view of a sensing area in a sensor film according to one implementation.

Referring to FIG. 3, the sensing area 150 includes a first pad 151, a second pad 152, a third pad 153, and a fourth pad 154 sequentially connected, and the sensing material It includes an antibody and a coloring agent for ELISA, the antibody and the coloring agent are placed on the second pad 152 and the third pad 153, respectively, and the sample to be analyzed is introduced into the first pad 151. It is transferred to the second pad 152, binds to the antibody in the second pad 152, is transferred to the third pad 153, and reacts with the coloring agent in the third pad 153 to develop color. After being generated, it can be absorbed into the fourth pad 154.

Non-sensing areas excluding the sensing area 150 may be protected by a cover film 115. In addition, it may be more advantageous in terms of product storage and stability if the sensing area 150 is protected by a cover film except for the sample introduction part. Specifically, among the sensing area 150, except for the first pad 151 for sample introduction, the second pad 152, third pad 153, and fourth pad 154 are covered with a cover film. can be protected by

[Biosensor]

A biosensor according to one embodiment includes the sensor film; first means for unwinding the sensor film; a second means for introducing a sample into the unwound sensor film; and a third means for analyzing the sample introduced into the sensor film.

Accordingly, the sensor film is unwound by the first means and transferred to the second means, a sample is introduced in the second means and transferred to the third means, and analysis of the sample is performed in the third means. You can.

The biosensor can solve the problem of continuous supply of test strips, which was previously difficult to automate, by using the sensor film, and when linked, even the sample introduction and analysis process can be automated with current technology, ultimately making the entire process automated. Biosensors can be implemented.

The first means may comprise, for example, one or more drive rolls. Specifically, the first means may include one or more drive rolls coupled with the sensor film, and may unwind the sensor film by driving the roll.

In addition, in the biosensor, the time required for the sensor film to be transferred from the second means to reach the third means is greater than the time required for reaction from the time the sample is introduced to the sensor film until analysis is possible. The unwinding and conveying speed of the sensor film can be adjusted to be the same or slower.

In this way, by controlling the unwinding speed of the sensor film in the biosensor, the sensing material can be induced to sufficiently react with the sample, and by arranging two or more types of sensing materials in parallel on the sensor film, various detection analyzes can be performed simultaneously. It can be done.

Additionally, the second means is not particularly limited as long as it is configured to introduce a sample into the unwound sensor film. For example, it may have a configuration capable of adsorbing, collecting, or extracting a sample and transporting it to the sensor film.

In addition, the third means can analyze the result of a sample introduced into the sensor film reacting with the sensor material. For example, when the sensor film uses ELISA technique, the third means is a means for measuring the degree of color development. It can be.

In addition, the biosensor may further include a fourth means for transmitting the information analyzed by the third means to an external terminal by wire or wirelessly. Accordingly, the biosensor can be used as an unmanned detection module and can be applied to bacterial management and health diagnosis by transmitting real-time information.

For example, the biosensor is introduced into the air conditioning and ventilation system of a building or vehicle and used to manage bacteria in the filter in real time, or is introduced into a bidet or urinal and used to diagnose health in real time by easily checking biometric information. It can be.

[Device - Combined with Filter]

Figure 4 shows a device equipped with a biosensor according to one embodiment, capable of measuring bacterial contamination of a filter.

Referring to FIG. 4, a device according to one embodiment includes the sensor film 100; A roll 210 for unwinding the sensor film 100; filter 510; a tape 310 that absorbs the air 520 passing through the filter 510 and introduces a sample into the sensor film 100; and a measuring device 410 that analyzes the sample introduced into the sensor film 100.

The operating principle of the device is that the air 520 that has passed through the filter 510 is first adsorbed on the tape 310, and is transferred according to the direction of movement of the tape 310 to the sensor film 100 to sample (foreign matter in the air). etc.) is introduced. Thereafter, as the sample reacts with the sensing material of the sensor film 100, it reaches the measuring device 410 as the sensor film progresses, and the sample reacted with the sensing material is analyzed in the measuring device 410. Afterwards, the sensor film 100 is wound back into a roll form to facilitate storage and replacement.

Accordingly, the device can be used in the air conditioning system of a building or vehicle to detect viruses, bacteria, or mold attached to the filter.

[Device - combined with bidet]

Figure 5 shows a device equipped with a biosensor according to another embodiment, which can be combined with a bidet and perform unmanned automated health diagnosis through urine.

Referring to FIG. 5, the device includes the sensor film 100; A roll 220 for unwinding the sensor film 100; bidet (600); A collector 320 connected to the bidet 600 and collecting urine to introduce the sample into the sensor film 100; and a measuring device 420 that analyzes the sample introduced into the sensor film 100.

Here, the bidet 600 includes a sample extraction tube 610 that sucks urine by attaching a small motor; A motor 620 that adjusts the length of winding and unwinding of the extraction tube; Small pressure pump (630); A transfer pipe 640 for transferring the sample; Detection module 650; and a pressure detection sensor 660.

The operating principle of the device is that, when a person sits on the bidet 600, the pressure is detected by the pressure sensor 660 and the motor 620 is operated to adjust the length of the sample extraction tube 610, which is normally located in the toilet cover. It is prepared to collect urine by stretching, and the urine is collected from the sample extraction pipe 610 and then transferred to the detection module 650 through the transfer pipe 640 by the small pressure pump 630.

Within the sensing module 650, a sample is introduced into the sensor film 100 by the collector 320, and then the sample reacts with the sensing material of the sensor film 100 and is transmitted to the measuring device 420 according to the progress of the sensor film. The sample that has arrived and reacted with the sensing material is analyzed in the measuring device 420. Afterwards, the sensor film 100 is wound back into a roll form to facilitate storage and replacement.

The device can perform health examination through urine analysis. Specifically, the device can analyze viruses, bacteria, mold, proteins, acidity, chemical components, cells, etc. in urine to diagnose kidney function and other health conditions. In addition, the device combines with wireless communication means to transmit health examination results to the individual's mobile phone or hospital, enabling personalized healthcare.

[Device - combined with urinal]

Figure 6 shows a device equipped with a biosensor according to another embodiment, which can be combined with a urinal to perform unmanned automated health examination.

Referring to FIG. 6, the device includes the sensor film 100; A roll 230 for unwinding the sensor film 100; urinal (700); A collector 330 that collects urine flowing on the inner wall of the urinal and introduces the sample into the sensor film 100; and a measuring device 430 that analyzes the sample introduced into the sensor film 100.

This type of device has the advantage of not requiring separate power for sample collection by collecting urine using natural fall.

The collector 330 may be specifically composed of three pipes. The first pipe collects samples flowing along the wall, and the second pipe collects samples falling vertically by drilling a hole along the center of the pipe, The third tube may serve to collect samples collected in the first and second tubes and introduce them into the sensor film 100.

The measuring device 430 can detect a change in the color of urine that reacts with the sensing material of the sensor film 100. At this time, by combining the device with a camera, IR sensor, and wireless communication device, the information detected by the measuring device 430 can be transmitted to an external display or mobile phone.

In addition, the device may further include a cutter 710 that cuts the sensor film 100 that has been analyzed in the measuring device 430. The biosensor can be managed hygienically by storing the cut sensor film in a separate collection bin and then disposing of it.

100: sensor film, 110: base film,
115: cover film, 120: sensing material,
130: Hall, 150: Sensing area,
150': first sensing area, 150'': second sensing area,
151: first pad, 152: second pad,
153: 3rd pad, 154: 4th pad,
210, 220, 230: roll, 310: tape,
320, 330: collector, 410, 420, 430: measuring instrument,
510: filter, 520: air,
600: bidet, 610: sample extraction tube,
620: motor, 630: small pressure pump,
640: transfer pipe, 650: detection module,
660: pressure sensor, 700: urinal,
710: Cutter.

Claims (20)

Base film that can be wound into a roll; and
Comprising a sensing material disposed on the surface of the base film,
A sensor film includes a plurality of sensing areas containing the sensing material,
The plurality of sensing areas are formed at regular intervals along the longitudinal direction of the base film,
The base film includes polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET), polycarbonate, polyvinyl chloride (PVC), or polylactic acid (PLA),
A sensor film in which the base film is bent more than 100 times in an MIT bending test until cutting.
According to claim 1,
A sensor film wherein the sensor film has a roll shape.
According to claim 2,
A sensor film, wherein the roll-shaped sensor film has a radius of curvature of 5R to 200R.
delete According to claim 1,
A sensor film wherein the sensing material is for detecting viruses, bacteria, or mold.
According to claim 1,
A sensor film wherein the sensing material includes an antibody for an antigen-antibody reaction.
According to claim 1,
A sensor film wherein the sensing material includes an antibody and a coloring agent for ELISA (enzyme-linked immunosorbent assay).
According to claim 1,
A sensor film wherein the sensor film includes two or more types of sensing materials for different detection targets, and the two or more types of sensing materials are arranged in parallel along the width direction of the base film.
delete According to claim 1,
The plurality of sensing areas
a first sensing area including a first sensing material for a first detection target, and
A sensor film comprising a second sensing area containing a second sensing material for a second detection target, wherein the first sensing area and the second sensing area are formed in parallel along the width direction of the base film.
According to claim 1,
The sensing area is
It includes a first pad, a second pad, a third pad, and a fourth pad connected in sequence,
The sensing material includes an antibody and a coloring agent for ELISA,
The antibody and the coloring agent are disposed on the second pad and the third pad, respectively,
The sample you want to analyze is
introduced into the first pad and transferred to the second pad,
binds to the antibody in the second pad and is transferred to the third pad,
After reacting with the coloring agent in the third pad to develop color,
A sensor film absorbed by the fourth pad.
The sensor film of claim 1;
first means for unwinding the sensor film;
a second means for introducing a sample into the unwound sensor film; and
A biosensor comprising a third means for analyzing a sample introduced into the sensor film.
According to claim 12,
The sensor film
Unwound by the first means and transferred to the second means,
A sample is introduced from the second means and transferred to the third means,
Analysis of the sample is performed in said third means,
So that the time required for the sensor film to be transferred from the second means and reach the third means is the same or slower than the time required for reaction from the time the sample is introduced to the sensor film to the point at which analysis is possible. Biosensor with controlled unwinding and transfer speed of the sensor film.
According to claim 12,
The biosensor
The biosensor further includes a fourth means for transmitting the information analyzed by the third means to an external terminal by wire or wirelessly.
The sensor film of claim 1;
a roll for unwinding the sensor film;
filter;
a tape that absorbs air passing through the filter and introduces a sample into the sensor film; and
A device comprising a measuring device that analyzes a sample introduced into the sensor film.
According to claim 15,
A device wherein the device is used in an air conditioning system of a building or vehicle to detect viruses, bacteria or mold attached to a filter.
The sensor film of claim 1;
a roll for unwinding the sensor film;
bidet;
A collector connected to the bidet and collecting urine to introduce the sample into the sensor film; and
A device comprising a measuring device that analyzes a sample introduced into the sensor film.
According to claim 17,
A device wherein the device performs health examination through urine analysis.
The sensor film of claim 1;
a roll for unwinding the sensor film;
urinal;
a collector that collects urine flowing on the inner wall of the urinal and introduces the sample into the sensor film; and
A device comprising a measuring device that analyzes a sample introduced into the sensor film.
According to claim 19,
The device
The device further comprises a cutter for cutting the sensor film on which analysis has been completed in the measuring device.

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