KR102459226B1 - An apparatus for manufacturing a well plate using micro-scale additive processing and dispensing a solution, and a method for manufacturing a well plate and dispensing a solution using the same - Google Patents

Abstract

An embodiment of the present invention provides a technique for manufacturing a well plate having high integration/high resolution using a micro-scale additive manufacturing process and dispensing a solution to such a well plate. A well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing according to an embodiment of the present invention includes a stage providing a surface on which a well plate having a plurality of unit structures is formed; an ink discharging unit having a plurality of ink nozzles discharging ink to form a well plate by ink on the surface of the stage; a solution dispensing unit having a plurality of solution nozzles for dispensing a solution to a well plate formed on the surface of the stage; a supply unit coupled to the ink dispensing unit and the solution dispensing unit, supplying ink to the ink dispensing unit and supplying a solution to the solution dispensing unit; and a transfer module coupled to the supply unit and three-dimensionally moving the supply unit.

Description

Well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, and well plate manufacturing and solution dispensing method using the same PLATE AND DISPENSING A SOLUTION USING THE SAME}

The present invention relates to a well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, and a well plate manufacturing and solution dispensing method using the same, and more particularly, to a well plate manufacturing and solution dispensing method using the micro-scale additive manufacturing process. It relates to a technique for manufacturing a well plate and dispensing a solution to such a well plate.

The well plate is used in various bio-related fields such as material screening (new drug), bio-reactor, and cell culture. The maximum resolution is determined according to the resolution.

Since the size of the droplet discharged from the micropipette has a minimum limit, due to this principle, a well plate having 1536 wells is a well plate with the highest resolution that is being commercialized. This limitation of structural resolution has a problem in that it is eventually connected with the limitation of throughput.

On the other hand, when the above experiment is performed using such a well plate, the characteristics of the individual using the micropipette are reflected in the results of the experiment. error is reduced to a minimum. However, this repeated experiment is itself a time/material loss, and there is a problem that a method is required to fundamentally block the change in results according to individual characteristics. In particular, when the resolution of the well plate is increased, the increase in the number of wells per unit area leads to a decrease in the size of individual wells. If so, the probability of error may increase.

In Korean Patent No. 10-1324478 (Name of the Invention: System and Method for Manufacturing High Aspect Ratio Nanowire Array Using Multiple Nozzles), a nanoscale structure is manufactured by providing a liquid raw material from a plurality of nozzles to the surface of the substrate. Forming and controlling the discharge of the liquid raw material through a plurality of nozzles is disclosed. However, in the case of the prior patent, there is a limit to forming a fine pattern for the well plate because the nanowire is manufactured from the liquid acid raw material discharged from a plurality of nozzles.

And, in US Patent Publication No. 2019/0048217 (title of the invention: Three-dimensional inkjet printing using ring-opening metathesis polymerization), at least one nozzle is provided, and the supply unit and the nozzle are connected by a predetermined tube, and each 3D printing by ink is performed by controlling the ejection of ink delivered to the nozzle, and the ink introduced into the chamber can be mixed and used. However, in the case of the prior patent, there is a problem in that a conventional nozzle size is used instead of using a fine nozzle or the like, and there is a limitation in forming a fine pattern of a three-dimensional structure suitable for an analyte.

Republic of Korea Patent Registration No. 10-1324478 US Patent Publication No. 2019/0048217 US Patent Publication No. 2018/0243478

An object of the present invention for solving the above problems is to manufacture a well plate having high integration/high resolution using a micro-scale additive manufacturing process.

In addition, it is an object of the present invention to dispense fine solution droplets to the well plate prepared as described above in a quantitative manner.

And, it is an object of the present invention to continuously perform the preparation of the well plate and the dispensing of the solution on the well plate.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those of ordinary skill in the art to which the present invention belongs from the description below. There will be.

A configuration of the present invention for achieving the above object includes a stage providing a surface on which a well plate having a plurality of unit structures is formed; an ink discharging unit having a plurality of ink nozzles discharging ink to form the well plate by ink on the surface of the stage; a solution dispensing unit having a plurality of solution nozzles for dispensing a solution to the well plate formed on the surface of the stage; a supply unit coupled to the ink dispensing unit and the solution dispensing unit, and supplying ink to the ink dispensing unit and supplying a solution to the solution dispensing unit; and a transfer module coupled to the supply unit and moving the supply unit three-dimensionally, wherein the formation of the well plate on one surface of the stage and dispensing of the solution on the surface of the well plate are sequentially performed.

In an embodiment of the present invention, the well plate may further include a substrate on which a plurality of the unit structures are formed on a surface.

In an embodiment of the present invention, a distance between one unit structure adjacent to each other and another unit structure may be 250 micrometers (㎛) or less.

In an embodiment of the present invention, the unit structure may be formed in one shape selected from a cone shape, a cylinder shape, a polygonal pillar, and a hemispherical shape.

In an embodiment of the present invention, the unit structure may be formed of any one or more materials selected from the group consisting of an elastic polymer material, a biomaterial, and a conductive material.

In an embodiment of the present invention, the transfer module comprises: a left and right transfer unit coupled to the supply unit and moving the supply unit in a left and right direction; a vertical transfer unit coupled to the left and right transfer unit and moving the left and right transfer unit in a vertical direction; and a forward and backward transfer unit coupled to the vertical transfer unit and moving the vertical transfer unit in the front-rear direction.

In an embodiment of the present invention, the supply unit, the ink supply for supplying ink to the ink discharge unit; and a solution supplier for supplying a solution to the solution dispensing unit.

In an embodiment of the present invention, the solution supplier may synthesize a plurality of drugs to generate a solution and then deliver the solution to the solution dispensing unit.

In an embodiment of the present invention, the ink discharging unit includes: an ink nozzle support coupled to a plurality of ink nozzles and delivering ink to the ink nozzles; and an ink delivery body having one end coupled to the ink nozzle support and the other end connected to the ink supplying body, and transferring the ink delivered from the ink supplying body to the ink nozzle support.

In an embodiment of the present invention, the solution dispensing unit, a solution nozzle support coupled to a plurality of solution nozzles and delivering the solution to the solution nozzles; and a solution delivery body having one end coupled to the solution nozzle support and the other end connected to the solution provider, and transferring the solution delivered from the solution provider to the solution nozzle support.

In an embodiment of the present invention, the diameter of the outlet of the ink nozzle may be 500 nanometers (nm) or less.

In an embodiment of the present invention, the diameter of the outlet of the solution nozzle may be 500 nanometers (nm) or less.

The configuration of the present invention for achieving the above object is a first step of preparing the ink delivered from the supply unit to the ink nozzle; a second step in which ink is ejected from the ink nozzle onto the surface of the stage and the ink nozzle is moved by the transfer module, whereby stacking of the ink is performed; a third step of drying the well plate formed by lamination of ink; a fourth step of preparing a solution delivered from the supply unit to the solution nozzle; and a fifth step of dispensing the solution from the dragon fruit nozzle onto the well plate.

The effect of the present invention according to the above configuration is that the number of wells formed per unit area is significantly increased because a well plate is manufactured using a nozzle having a fine outlet diameter. that can be manufactured.

In addition, an effect of the present invention is that, since the well plate formed as described above and having a function of high integration and high resolution is used, the precision of solution analysis is improved and the absolute number of analysis experiments is significantly increased.

And, the effect of the present invention is that, since the solution to be analyzed is dispensed on the well plate using a plurality of fine nozzles, it is possible to improve the analysis precision of the solution by realizing the quantitative discharge of the solution.

The effects of the present invention are not limited to the above effects, and it should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a perspective view of a well plate manufacturing and solution dispensing apparatus according to an embodiment of the present invention.
2 is a schematic diagram of an ink discharging unit according to an embodiment of the present invention.
3 is a schematic diagram of a solution dispensing unit according to an embodiment of the present invention.
4 is a schematic diagram of an ink dispensing unit and a solution dispensing unit according to an embodiment of the present invention.
5 is a cross-sectional view of an ink nozzle according to another embodiment of the present invention.
6 is an image of a unit structure according to an embodiment of the present invention.
7 is an image of a unit structure according to another embodiment of the present invention.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention may be embodied in several different forms, and thus is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout the specification, when a part is "connected (connected, contacted, coupled)" with another part, it is not only "directly connected" but also "indirectly connected" with another member interposed therebetween. "Including cases where In addition, when a part "includes" a certain component, this means that other components may be further provided without excluding other components unless otherwise stated.

The terms used herein are used only to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In the present specification, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It is to be understood that this does not preclude the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

1 is a perspective view of a well plate manufacturing and solution dispensing apparatus according to an embodiment of the present invention, FIG. 2 is a schematic diagram of an ink discharging unit 100 according to an embodiment of the present invention, and FIG. 3 is a diagram of the present invention It is a schematic diagram of the solution dispensing unit 200 according to an embodiment. And, FIG. 4 is a schematic diagram of the ink discharging unit 100 and the solution dispensing unit 200 according to an embodiment of the present invention.

1 to 4, the well plate manufacturing and solution dispensing apparatus of the present invention includes a stage 500 for providing a surface on which a well plate 10 including a plurality of unit structures 11 is formed; an ink discharging unit 100 having a plurality of ink nozzles 110 discharging ink to form a well plate 10 by ink on the surface of the stage 500; a solution dispensing unit 200 having a plurality of solution nozzles 210 for dispensing a solution to the well plate 10 formed on the surface of the stage 500; a supply unit coupled to the ink dispensing unit 100 and the solution dispensing unit 200 , and supplying ink to the ink dispensing unit 100 and supplying a solution to the solution dispensing unit 200 ; and a transfer module coupled to the supply unit and three-dimensionally moving the supply unit. Cells, drugs, etc. may be included in the solution, and by dispensing the solution into the well plate 10 , the well plate 10 for solution analysis may be prepared.

Here, the formation of the well plate 10 on the surface of one stage 500 and the dispensing of the solution on the surface of the well plate 10 may be sequentially performed. In this way, since the preparation of the well plate 10 and the dispensing of the solution on the well plate 10 can be performed in one device, analysis efficiency can be improved in time and space for analysis of the solution.

In addition, since the solution to be analyzed is dispensed on the well plate 10 using a plurality of fine nozzles, it is possible to improve the analysis precision of the solution by realizing the quantitative discharge of the solution.

The well plate 10 may further include a substrate 12 having a plurality of unit structures 11 formed on the surface thereof. In addition, a unit structure 11, which is a fine structure formed on the substrate 12, may be formed, wherein the interval between one unit structure 11 and the other unit structure 11 adjacent to each other is 250 micrometers ( μm) or less. In addition, in the plan view of one unit structure 11 , the longest length among the lengths connecting one point and another point may be 2 millimeters (mm) or less.

In order to form the unit structure 11 as described above, the diameter of the ink nozzle 110 may be formed to be 500 nanometers (nm) or less, and a plurality of ink droplets discharged from the ink nozzle 110 are combined to form one of the unit structure 11 may be formed.

And, also in the solution nozzle 210, the outlet diameter of the solution nozzle 210 may be formed to be 500 nanometers (nm) or less, and accordingly, the solution droplets discharged from the solution nozzle 210 are quantified in minute capacity units. It can be dispensed, the precision of the solution dispensing amount can be improved.

As described above, as the longest length of the unit structures 11 and the spacing between the adjacent unit structures 11 are formed, the number of wells formed per unit area on the substrate 12 may increase.

That is, a plurality of wells may be formed in the well plate 10 , and a space between each of the plurality of unit structures 11 formed on the substrate 12 may be a well, and the number of unit structures 11 increases. The number of wells may be increased according to the The number of wells formed per unit area of the phase may be increased.

Specifically, more than 5,000 wells (specifically, 6144-well (1536*4)) can be formed in the same size as the well plate 10 having 1,536 wells that are currently commercially available and used. Accordingly, the well plate 10 of the present invention can be provided with a function of high integration and high resolution, so that the analysis precision using the well plate 10 of the present invention is remarkably improved, and at the same time, the absolute analysis experiment that can be performed at once The number can be significantly increased.

The substrate 12 functions as a support member for supporting the plurality of unit structures 11, and the substrate 12 has a plate shape, which can support the unit structures 11 such as polymer materials, glass, and ceramics. structure can be formed.

In the embodiment of the present invention, it is described that the substrate 12 is formed separately and the unit structure 11 is formed on the substrate 12 , but the present invention is not limited thereto, and the substrate 12 and the unit structure are formed by lamination of ink. (11) may be simultaneously formed of the same material.

The unit structure 11 may be formed of any one or more materials selected from the group consisting of an elastic polymer material, a biocompatible material, and a conductive material. Here, PDMS, Polyurethane, etc. may be used as the elastic polymer material, Biocompatibility polymer, ceramics such as SiO ₂ may be used as the biocompatible material, and CNT, PEDOT: PSS, etc. may be used as the conductive material. have.

However, in the embodiment of the present invention, it is described that the unit structure 11 is formed of the above material, but the present invention is not limited thereto, and any material suitable for fixing fine particles such as cells and proteins may be used.

The unit structure 11 may be formed in one shape selected from a cone shape, a cylinder shape, a polygonal pillar shape, and a hemispherical shape. When the unit structure 11 is formed into a predetermined shape as described above, the shape of the bottom or wall can be made to have a cell or protein-friendly three-dimensional structure, and the solution is included in the solution when dispensing the solution into the well plate 10 . A material such as cells or proteins may be seated on the well plate 10 .

Fine ink droplets may be discharged from the ink nozzle 110 onto the substrate 12, and the unit structure 11 is formed by stacking such fine ink droplets, so that the unit structure 11 has a predetermined shape as described above. may be easy to provide.

The transfer module includes: a left and right transfer unit coupled to the supply unit and moving the supply unit in a left and right direction; a vertical transfer unit coupled to the left and right transfer unit and moving the left and right transfer unit in the vertical direction; And it may include a forward and backward transfer unit coupled with the vertical transfer unit and moves the vertical transfer unit in the front-rear direction.

The left and right transfer unit includes a left and right actuator 411 that is coupled to the supply unit and moves the supply unit left and right, and a left and right guide body 412 coupled with the left and right actuator 411 and guides the left and right movement of the left and right actuator 411 can do.

In addition, the vertical transfer unit is coupled to the left end of the left and right guide body 412 and the first up and down drive body 421 for moving the left and right guide body 412 up and down, and the right end of the left and right guide body 412 coupled with the left and right guide body The second vertical actuator 422 for moving the 412 up and down, the first vertical guide body 423 coupled with the first vertical actuator 421 and guiding the vertical movement of the first vertical actuator 421, 423; and a second vertical guide body 424 coupled to the second vertical actuator 422 and guiding the vertical movement of the second vertical actuator 422 .

In addition, the front and rear transfer unit is coupled to the lower end of the first upper and lower guide body 423 and moves the first up and down guide body 423 back and forth. A second front and rear actuator 432 for coupling with and moving the second up and down guide body 424 forward and backward, and a first for guiding the front and rear movement of the first front and rear actuator 431 and coupled with the first front and rear actuator 431 A first front and rear guide body 433 and a second front and rear guide body 434 coupled to the second front and rear actuators 432 and guiding the forward and backward movement of the second front and rear actuators 432 may be provided.

Here, the left and right actuators 411 , the first upper and lower actuators 421 , the second upper and lower actuators 422 , the first front and rear actuators 431 , and the second front and rear actuators 432 each have a motor. A pinion gear coupled to the motor of each driving body is meshed with a rack gear formed in each guide body, so that each driving body can be moved according to the operation of the motor.

By forming the transfer module as described above, the supply unit coupled to the transfer module can perform precise three-dimensional movement, and accordingly, the ink nozzle 110 of the ink discharge unit 100 connected to the supply unit and the solution dispensing unit ( 200), precise three-dimensional movement of the solution nozzle 210 is possible, so that the positions of each of the stacking of the ink and the dispensing of the solution can be precisely controlled.

The supply unit, the ink supply unit 310 for supplying ink to the ink discharge unit 100; and a solution supplier 320 for supplying a solution to the solution dispensing unit 200 . Here, the solution supplier 320 may synthesize a plurality of drugs to generate a solution and then deliver the solution to the solution dispensing unit 200 . Similarly, the ink supplier 310 may mix a plurality of ink materials to generate ink, and then transfer the ink to the ink discharge unit 100 .

The ink discharge unit 100 includes an ink nozzle support 120 coupled to a plurality of ink nozzles 110 and delivering ink to the ink nozzles 110 ; and an ink delivery body 130 having one end coupled to the ink nozzle support 120 and the other end connected to the ink supply 310 and transferring the ink delivered from the ink supply 310 to the ink nozzle support 120 . can do.

An ink delivery tube for providing a flow path to flowing ink is formed inside the ink delivery body 130 , and the ink delivery tube receives ink from the ink supply body 310 and passes the ink therethrough, and then the ink nozzle support 120 . can be passed to A plurality of ink channels that are channels connected to the ink delivery tube may be formed in the ink nozzle support 120 , and each ink channel is connected to each ink nozzle 110 to transfer the ink delivered from the ink delivery tube to the ink nozzle ( 110) can be forwarded.

The solution dispensing unit 200 includes a solution nozzle support 220 coupled to a plurality of solution nozzles 210 and delivering the solution to the solution nozzles 210; and a solution delivery body 230 having one end coupled to the solution nozzle support 220 and the other end connected to the solution provider 320 and transferring the solution delivered from the solution provider 320 to the solution nozzle support 220 . can do.

A solution delivery pipe providing a flow path to the flowing solution is formed inside the solution delivery body 230 , and the solution delivery pipe receives the solution from the solution provider 320 and passes the solution through the solution nozzle support 220 . can be passed to A plurality of solution channels, which are channels connected to the solution delivery pipe, may be formed in the solution nozzle support 220, and each solution channel is connected to each solution nozzle 210 to receive the solution delivered from the solution delivery pipe through the solution nozzle ( 210) can be transferred.

As shown in FIG. 1 , the ink delivery body 130 may be formed in front of the solution delivery body 230 , and the ink delivery body 130 may have a bent portion. With such a configuration, the ink discharging unit 100 and the solution dispensing unit 200 may be located separately from each other, and even if lamination by ink and dispensing of a solution are performed in one device, the ink dispensing unit 100 and Interference of the solution dispensing unit 200 can be minimized.

And, as described above, a plurality of ink channels are formed in the ink nozzle support 120 and a plurality of solution channels are formed in the solution nozzle support 220, so that each ink nozzle 110 and each Since a fine amount of ink or solution can be precisely transferred to the solution nozzle 210 in a quantitative manner, the manufacturing precision of the well plate 10 and the solution dispensing precision can be improved.

5 is a cross-sectional view of an ink nozzle according to another embodiment of the present invention. As shown in Figure 5, the ink nozzle 110, the sheath re-discharge tube 112 is formed through the inside along the longitudinal central axis; It is provided in the ink nozzle 110 so as to penetrate the body of the ink nozzle 110, the pattern re-discharge tube 111 is formed along the inside of the sheath re-discharge tube 112; may be provided. Here, the sheath (sheath) may be used in the meaning of a covering covering.

A portion of the pattern re-discharge tube 111 located inside the sheath re-discharge tube 112 and the sheath re-discharge tube 112 coincide with each other in the longitudinal direction of the central axis, and pass through the pattern re-discharge tube 111 . When discharging the pattern material, the sheath material wraps the discharge line of the pattern material, so that it can be discharged in the form of a coaxial concentric circle in the cross section.

Each of the material for the sheath and the material for the pattern is included in the ink, and the material for the sheath may be a biodegradable polymer material. In addition, the material for the sheath may be a material that does not mix with the material for the pattern. The material for the sheath and the material for the pattern must each have a property that does not mix, so that the material for the sheath and the material for the pattern can be continuously discharged in the shape that the material for the sheath is wrapped around the discharge line of the material for the pattern as described above.

The pattern material may include an elastic polymer or a conductive polymer. Specifically, the type of material for a pattern may vary depending on the purpose of printing, and the material for a pattern may include an elastic polymer such as PDMS (Polydimethylsiloane) in order to manufacture an article having a stretchable function.

In addition, in order to manufacture an article having electrical conductivity, the material for a pattern may include a material such as CNT, AgNW, Ag flake, conductive polymer, or the like. And, in order to perform particle unit printing, the pattern material may include a polymer or a solution on which the particles are supported. Here, the particles may be inorganic particles such as metal or organic particles such as cells.

Biodegradable polymer materials include PCL (Poly CaproLactone), PLA (Poly Lactic Acid), PLLA (Poly L-Lactic Acid), PEO (poly ethylene oxide), PVA (poly viny lacetate), PLGA (poly (lactic-co-) glycolic acid)), collagen, chitosan, etc. may be used. Here, an enzyme may be used to remove the biodegradable polymer material, which is a prior art and a detailed description thereof will be omitted.

As described above, since the material for the sheath, which is a biodegradable polymer material, is discharged while wrapping the material for the pattern for the formation of a nano-level line width pattern, unlike the method of venting air around the material for the pattern, the material for the sheath is stably patterned It guides the formation of the diameter of the material for the material, and the influence on the material for the sheath is minimized even in adjusting the distance between the ink nozzle 110 and the substrate, so that it is easy to form a nano-unit pattern by the material for the pattern.

When the ink nozzle 110 is formed as described above, the ink supply body 310 may include a pattern re-supplier for supplying a pattern material and a sheath re-supply body for supplying the sheath material. In addition, the ink delivery body 130 is connected to the pattern re-supply body to receive the pattern material and the pattern re-delivery pipe for transferring the pattern material to the ink nozzle support 120, and the sheath body supplier to receive the material for the sheath. It may be provided with a sheath re-transmission pipe for transferring the material for the sheath to the ink nozzle support 120 .

In addition, in the ink nozzle support 120 , a plurality of pattern material channels are formed in the same channel as the above ink channels, which are connected to the pattern re-transmission tube and transfer the material for the pattern to the pattern re-discharge tube 111 , and the sheath re-discharge. A plurality of sheath material channels that are connected to the dalgwan to deliver the sheath material to the sheath re-discharge pipe 112 may be formed.

Accordingly, the pattern material discharged from the pattern re-supplier sequentially passes through the pattern re-delivery pipe, the pattern material channel, and the pattern re-discharge tube 111, and the sheath material discharged from the sheath re-supplier is discharged from the sheath re-discharge. It may be discharged through the dalgwan and the sheath material channel and the sheath material discharge tube 112 sequentially.

6 is an image of the unit structure 11 according to an embodiment of the present invention, and FIG. 7 is an image of the unit structure 11 according to another embodiment of the present invention. Here, FIGS. 6 (a) and (b) are images obtained by capturing the unit structures 11 formed in a cylindrical shape at different positions on the substrate 12, and FIGS. 7 (a) to (c) are , It is an image of the unit structure 11 formed in the shape of a square pillar at different positions on the substrate 12, respectively.

As shown in FIG. 6 , the longest length of the unit structures 11 may be formed to be 580 micrometers (㎛) or less, and the interval between the unit structures 11 may be formed to be 250 micrometers (㎛) or less. can be checked And, as shown in FIG. 7 , the longest length of the unit structures 11 may be formed to be 1.22 millimeters (mm) or less, and the distance between the unit structures 11 may be formed to be 250 micrometers (㎛) or less. can confirm.

As described above, when using the well plate manufacturing and solution dispensing apparatus of the present invention, it is confirmed that the well plate 10 in which a plurality of fine wells are formed by the plurality of unit structures 11 on the substrate 12 can be manufactured. can

It is possible to form a biomaterial analysis system including the well plate manufacturing and solution dispensing device of the present invention as described above.

Hereinafter, a well plate manufacturing and solution dispensing method using the well plate manufacturing and solution dispensing apparatus of the present invention will be described.

In the first step, the ink transferred from the supply unit to the ink nozzle 110 may be prepared.

And, in the second step, ink is discharged from the ink nozzle 110 onto the surface of the stage 500 and the ink nozzle 110 is moved by the transfer module, so that the ink lamination can be performed.

Next, in the third step, the well plate 10 formed by lamination of ink may be dried. Here, the well plate 10 may be naturally dried or dried by thermal energy transferred to the stage 500 .

After that, in the fourth step, it is possible to prepare a solution delivered from the supply unit to the solution nozzle (210). And, in the fifth step, the solution may be dispensed onto the well plate 10 from the dragon fruit nozzle.

The rest of the well plate manufacturing and solution dispensing method of the present invention are the same as those expected for the well plate manufacturing and solution dispensing apparatus of the present invention described above.

The foregoing description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and likewise components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

10: well plate 11: unit structure
12: substrate 100: ink ejection unit
110: ink nozzle 111: pattern re-discharge pipe
112: sheath material discharge pipe 120: ink nozzle support
130: ink delivery body 200: solution dispensing part
210: solution nozzle 220: solution nozzle support
230: solution delivery body 310: ink supply body
320: solution supplier 411: left and right actuator
412: left and right guide body 421: first vertical drive body
422: second upper and lower drive body 423: first upper and lower guide body
424: second upper and lower guide body 431: first front and rear drive body
432: second front and rear driving body 433: first front and rear guide body
434: second front and rear guide body 500: stage

Claims (14)

a stage providing a surface on which a well plate including a plurality of unit structures is formed;
an ink discharging unit having a plurality of ink nozzles discharging ink to form the well plate by ink on the surface of the stage;
a solution dispensing unit having a plurality of solution nozzles for dispensing a solution to the well plate formed on the surface of the stage;
a supply unit coupled to the ink dispensing unit and the solution dispensing unit, and supplying ink to the ink dispensing unit and supplying a solution to the solution dispensing unit; and
Containing; and a transfer module coupled to the supply unit and moving the supply unit in three dimensions;
Formation of the well plate on the surface of one of the stages and dispensing of the solution on the surface of the well plate are sequentially performed,
The ink nozzle is a tube formed through the inside along the central axis in the longitudinal direction of the ink nozzle, and a sheath re-discharge tube through which a material for a sheath, which is ink, passes through, and a portion is formed along the inside of the sheath re-discharge tube A pattern re-discharging tube through which a material for a pattern, which is ink, is passed as a tube,
When the pattern material is discharged, the sheath material wraps around the discharge line of the pattern material, so that the pattern material and the sheath material are discharged in a coaxial concentric circle shape in cross section,
The material for the sheath is a well plate manufacturing and solution dispensing device using micro-scale additive manufacturing, characterized in that it is a biodegradable polymer material.
The method according to claim 1,
The well plate, a well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that it further comprises a substrate on which a plurality of the unit structures are formed on the surface.
The method according to claim 1,
A well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that the interval between one unit structure adjacent to each other and another unit structure is 250 micrometers (㎛) or less.
The method according to claim 1,
The unit structure is a well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that it is formed in one shape selected from a conical shape, a cylinder, a polygonal pillar, and a hemispherical shape.
The method according to claim 1,
The unit structure is a well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that it is formed of any one or more materials selected from the group consisting of an elastic polymer material, a biomaterial, and a conductive material.
The method according to claim 1,
The transfer module is
a left and right transfer unit coupled to the supply unit and moving the supply unit in a left and right direction;
a vertical transfer unit coupled to the left and right transfer unit and moving the left and right transfer unit in a vertical direction; and
A well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that it includes a forward and backward transfer unit coupled to the vertical transfer unit and moving the vertical transfer unit in the front-rear direction.
The method according to claim 1,
The supply unit,
an ink supply body for supplying ink to the ink discharge unit; and
A well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that it comprises a solution supplier for supplying a solution to the solution dispensing unit.
8. The method of claim 7,
The solution supplier synthesizes a plurality of drugs to generate a solution, and then delivers the solution to the solution dispensing unit.
8. The method of claim 7,
The ink discharge unit,
an ink nozzle support coupled to a plurality of ink nozzles and delivering ink to the ink nozzles; and
A well using micro-scale additive manufacturing, characterized in that it comprises an ink delivery body that has one end coupled to the ink nozzle support and the other end connected to the ink supply body, and delivers the ink delivered from the ink supply body to the ink nozzle support. Plate making and solution dispensing device.
8. The method of claim 7,
The solution dispensing unit,
a solution nozzle support coupled to a plurality of solution nozzles and delivering a solution to the solution nozzles; and
A well using micro-scale additive manufacturing, characterized in that it comprises a solution delivery body that has one end coupled to the solution nozzle support and the other end connected to the solution provider, and delivers the solution delivered from the solution provider to the solution nozzle support. Plate making and solution dispensing device.
The method according to claim 1,
The diameter of the ink nozzle is 500 nanometers (nm) or less well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that.
The method according to claim 1,
A well plate manufacturing and solution dispensing apparatus using micro-scale additive manufacturing, characterized in that the outlet diameter of the solution nozzle is 500 nanometers (nm) or less.
A biomaterial analysis system comprising a well plate manufacturing and solution dispensing device using the micro-scale additive manufacturing according to any one of claims 1 to 12.
In the well plate manufacturing and solution dispensing method using the well plate manufacturing and solution dispensing apparatus using the micro-scale additive manufacturing of claim 1,
a first step of preparing ink to be delivered from the supply unit to the ink nozzle;
a second step in which ink is ejected from the ink nozzle onto the surface of the stage and the ink nozzle is moved by the transfer module, whereby stacking of the ink is performed;
a third step of drying the well plate formed by lamination of ink;
a fourth step of preparing a solution delivered from the supply unit to the solution nozzle; and
A fifth step of dispensing a solution from the solution nozzle onto the well plate.

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