KR102427990B1 - Fo-lspr diagnostic system for biomolecule, comprising disposable optical fiber sensor and cartridge thereof - Google Patents

Abstract

An optical fiber sensor used in connection with FO-LSPR equipment and a disposable sensor cartridge capable of mounting the same are presented. The disposable sensor cartridge of the present invention includes a cartridge body having a reaction chamber, a sensor insertion groove, a fluid passage and a waste reservoir, and a cartridge cover having a sample inlet and a fluid outlet, and after mounting the optical fiber sensor, connect the FO-LSPR equipment Therefore, it can be used for rapid detection of biomolecules such as nucleic acids of viruses. In addition, the sensor cartridge of the present invention is easily detachable from the FO-LSPR equipment, so it is convenient to use, and by adopting a plastic material, the production cost is low and mass production is easy.

Description

FO-LSPR DIAGNOSTIC SYSTEM FOR BIOMOLECULE, COMPRISING DISPOSABLE OPTICAL FIBER SENSOR AND CARTRIDGE THEREOF

The present invention relates to a disposable optical fiber sensor and a cartridge thereof, and more particularly, to a disposable optical fiber that can be used for rapid and convenient diagnosis of infectious diseases in connection with optical detection equipment such as FO-LSPR (fiber optics-localized surface plasmon resonance) It relates to a sensor and a cartridge thereof.

A biosensor is an analysis device that detects changes in the concentration or presence of biomaterials. Usually, a ligand capable of binding biomolecules such as antigens or nucleic acids is attached to the sensor to detect physicochemical changes caused by binding of biomolecules to the ligand. used to measure These physicochemical changes include electrochemical, surface plasmon resonance (SPR), localized surface plasmon resonance (LSPR), field effect transistor (FET), fluorescence, and quartz crystal microbalance (QCM). etc.

LSPR is a phenomenon in which electrons collectively vibrate due to the interaction of light and electrons at the boundary between the metal surface and the dielectric when light is irradiated to fine metal nanoparticles (Willets and Van Duyne, Annu rev Phys Chem 58:267-297 , 2007; Petryayeva and Krull, Anal Chim Acta., 706: 8-24, 2011). When biomolecules are fixed on the surface of these metal nanoparticles and other molecules capable of binding to them are added to bind them, the refractive index of the surrounding medium changes, and thus the wavelength at which resonance occurs is changed, and this change is detected with a detection device.

As a diagnostic method for detecting a specific pathogen or virus, such as an infectious disease, immunodiagnostics and molecular diagnostics may be used. A representative technique of molecular diagnostics is PCR, which is widely used for nucleic acid detection. However, this method has limitations in point-of-care testing (POCT) because it requires sample manipulation, requires a lot of cost and time, requires large equipment, and may be contaminated during sample manipulation.

Therefore, for on-site diagnosis, there is a demand for a method for simple sample manipulation and a method for rapidly detecting a target material without the need for large equipment or a device capable of implementing the same.

The inventors of the present invention and the like have tried to meet the needs of the industry by developing a disposable optical fiber sensor cartridge that can be used in connection with a small-scale diagnostic equipment used in the field.

Prior Patent Document 10-1847745

An object of the present invention is to provide a disposable optical fiber sensor and a cartridge thereof that can be used in conjunction with a small-scale optical detection device to diagnose infectious diseases and the like.

Another object of the present invention is to provide a disposable optical fiber sensor cartridge made of a plastic material and easily mass-produced.

Another object of the present invention is to provide a disposable optical fiber sensor member that can be customized to the cartridge.

Another object of the present invention is to provide a biomolecular diagnostic system using FO-LSPR, including a disposable optical fiber sensor member and a cartridge thereof.

One aspect of the present invention is

A disposable sensor cartridge for mounting a fiber optic sensor member, comprising:

a cartridge body having a reaction chamber, a sensor insertion groove, a fluid passageway and a waste reservoir formed therein;

A disposable sensor cartridge may be provided, comprising a cartridge cover having a sample inlet and a fluid outlet formed therein.

In one embodiment of the present invention, the cartridge is a cartridge body including a reaction chamber, a first sensor insertion groove, a first fluid passage and a waste reservoir, and a sample inlet, a sample outlet, and a fluid discharged from the sample outlet on the upper surface of the waste A cartridge cover having a waste inlet for introducing it into the reservoir and having a second fluid passage corresponding to the first fluid passage, a waste inlet, and a second sensor insertion groove corresponding to the first sensor insertion groove on the back surface of the cartridge cover. may include

The inflow of the fluid into the waste storage area may be caused by forming a portion of the upper surface of the cover including the sample outlet portion as an inclined surface.

In one embodiment, the cartridge includes a reaction chamber, a sensor insertion groove, a fluid passageway, and a connection passageway. and a cartridge body having a waste reservoir and a sample inlet and an air outlet formed on the upper surface, and a first protrusion corresponding to the fluid passage and a second protrusion corresponding to the sensor insertion groove on the rear surface of the cartridge It may include a cover.

In one embodiment, the cover and the body of the sensor cartridge may be folded or separated from each other.

In one embodiment, a groove in which an optical fiber sensor member is disposed may be further formed in the body and/or cover of the cartridge.

Another aspect of the present invention may present an optical fiber sensor member mounted on the sensor cartridge described above.

In one embodiment, the optical fiber included in the optical fiber sensor member may be one or more. At the end of the optical fiber, a DNA oligomer, an RNA oligomer, Metal nanoparticles to which antigens or antibodies are attached may be bound.

In one embodiment, when the optical fiber sensor member has two optical fibers included in the optical fiber sensor member, a metal nanoparticle having an ssDNA oligomer attached to the end of one optical fiber and a metal nanoparticle having an ssRNA oligomer attached to the end of the other optical fiber The particles may be bound.

According to another aspect of the present invention, a biomolecule diagnosis system using FO-LSPR including the disposable sensor cartridge and an optical fiber sensor member mounted thereon can be provided.

The disposable sensor cartridge of the present invention can be used for rapid detection of biomolecules for diagnosing infectious diseases by connecting FO-LSPR equipment after mounting an optical fiber sensor. The sensor cartridge of the present invention is easily detachable from the FO-LSPR equipment, so it is convenient to use, and by adopting a plastic material, the production cost is low and mass production is easy.

1 and 2 are schematic diagrams showing the assembled state of the sensor cartridge 1 and the optical fiber sensor member 31 in a state in which the cover (A ') and the body (A) are folded according to an embodiment of the present invention,
Figure 3 is a schematic diagram showing the names of the body (A) and the name of each part on the back of the cover when the cover (A ') of the sensor cartridge according to an embodiment of the present invention is folded,
4 is a schematic view sequentially showing the process of mounting the optical fiber sensor member 31 to the sensor cartridge according to an embodiment of the present invention, and connecting the adapter 34 to the other end of the sensor member 31 after closing the cover. ego,
5 is a schematic diagram showing the relationship in which the optical fiber sensor member 41 and the adapter 42 are connected to the sensor cartridges (B, B') according to an embodiment of the present invention;
Figure 6 is a perspective view showing the back of the sensor cartridge body (B) and the cover (B ') according to an embodiment of the present invention,
7 is a schematic view from above of an aspect in which the optical fiber sensor member 41 is mounted on the sensor cartridge body (B) according to an embodiment of the present invention;
8 is a schematic diagram showing an assembled state of the sensor cartridges (B, B'), the optical fiber sensor member 41 and the adapter 42 according to an embodiment of the present invention,
9 is a schematic diagram showing an operation mode by inserting the sensor cartridge according to an embodiment of the present invention into the main body case of the FO-LSPR equipment including a computer equipped with a laser light source, a detector, and a signal analysis program.

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, these are presented as preferred examples of the present invention and cannot be construed as limiting the present invention for any reason.

Content not described herein will be omitted because it can be technically inferred sufficiently by a person skilled in the art to which the present invention pertains.

In the present invention, the term "diagnosis" refers to confirming the presence or absence of a disease or disease-related bacteria or virus.

In the present invention, the term "detection" includes quantitative and/or qualitative analysis, and includes detection of the presence or absence of a substance, and detection of the amount (level) present.

As used herein, the term "specimen sample" refers to a biological sample isolated from a subject or a liquid fluid including the sample, and includes, for example, blood, saliva, urine, and mucosal secretions from the back of the nose collected from a subject.

As used herein, the term "fluid" includes a sample sample, a buffer, a washing solution, or air.

One embodiment of the present invention is a disposable sensor cartridge for mounting a fiber optic sensor member, comprising a cartridge body having a reaction chamber, a sensor insertion groove, a fluid passage and a waste reservoir formed therein, and a cartridge cover having a sample inlet and a fluid outlet formed therein. sensor cartridge. The sensor cartridge is used for rapid detection of a target material for diagnosing an infectious disease in connection with an optical fiber sensor and FO-LSPR equipment.

In one embodiment, the sensor cartridge includes a cartridge body (A) having a reaction chamber (26), a first sensor insertion groove (27), a first fluid passage (28) and a waste reservoir (29) formed therein, and a sample on the upper surface The inlet 11, the sample outlet 12, and the waste inlet 13 for introducing the fluid from the sample outlet into the waste reservoir are formed, and a second fluid passage corresponding to the first fluid passage ( 24), the waist inlet 23 and the cartridge cover (A') in which the second sensor insertion groove 25 corresponding to the first sensor insertion groove is formed. The inflow of the waste fluid into the reservoir may be effected by forming a portion of the upper surface of the cover including the sample outlet portion to be inclined.

In one embodiment, the sensor cartridge has a cartridge body (B) and an upper surface in which a reaction chamber (46), a sensor insertion groove (47), a fluid passage (48) and a connection passage (49) and a waste reservoir (50) are formed. A sample inlet 51 and an air outlet 52 are formed in the back surface, and a first protrusion 57 corresponding to the fluid passage 48 and a second protrusion corresponding to the sensor insertion groove 47 ( 58) may include a cartridge cover (B') provided with. Here, the protrusion height of the first protrusion 57 may be approximately 70 to 90% of the depth of the fluid passage. When the washing liquid passes through the fluid passage 48 and is injected into the lower end of the reaction chamber 46 through the structure having the protrusion, the fluid exchange can occur more easily by pushing up the fluid existing in the center of the reaction chamber. The second protrusion 58 may serve to hold the two strands of optical fiber positioned in the sensor insertion groove 47 , prevent an erroneous overflow of fluid in the reaction chamber 46 , and fix the cartridge body and the cover.

In one embodiment, the cover and the body of the sensor cartridge may have a shape that is folded with each other. In this case, when the cover and the body are folded, the back surface of the cover and the body abut at respective positions corresponding to each other to form a complete space or passage (FIG. 4). For example, the first fluid passage 28 of the cartridge body and the second fluid passage 24 on the back surface of the cover contact to form a complete fluid passage, and the first sensor insertion groove 27 of the body and the second fluid passage 24 on the back surface of the cover The sensor insertion groove 25 abuts to form a complete sensor insertion groove.

Each part of the back surface of the body and cover of the sensor cartridge of the present invention may be formed in an intaglio except for the opening and the protrusion.

In one embodiment, the cover and the body may be in a separate form (FIG. 5). In this case, the cover and the body may be coupled with a connecting part, or may have concave-convex portions corresponding to each other.

In one embodiment, a groove in which an optical fiber sensor member is disposed may be further formed in the body and/or cover of the sensor cartridge.

The flow of fluid in the sensor cartridge of the present invention is as follows.

When the fluid is injected through the sample inlets 11 and 51 with the cover of the sensor cartridge covered, the fluid is collected into the area of the body corresponding to the sample inlet of the cover and then passes through the fluid passage to the reaction chambers 26 and 46. Move. According to one embodiment, the fluid moves to the waste reservoir 29 through the sample outlet 12 after contacting the optical fiber ends located in the reaction chambers 26 and 46 . According to another embodiment, the fluid moves to the waste storage 50 via the connecting passage 49 after contacting the optical fiber end. In this case, the air that can be injected into the cartridge together with the sample exits to the outside through the air outlet (52). The waste reservoirs 29 and 50 are areas for storing excess fluid that has passed through the reaction chamber, through which nucleic acids or antigens that do not participate in the reaction with the reporter molecule of the optical fiber sensor can be removed by injecting a washing solution.

One embodiment of another aspect of the present invention is the optical fiber sensor members 31 and 41 mounted on the sensor cartridge described above.

In one embodiment, the optical fiber included in the optical fiber sensor member 31 may be one.

In one embodiment, there may be two optical fibers included in the optical fiber sensor member 41 .

The optical fiber sensor member is mounted to the sensor cartridge such that the exposed optical fiber end is positioned within the reaction chamber of the sensor cartridge, as shown in FIGS. 4 and 7 . Metal nanoparticles to which a reporter molecule is attached are electrostatically coupled to the end of the optical fiber. The metal nanoparticles may be, for example, gold or silver nanoparticles having a spherical shape of about 50 nm.

The reporter molecule acts as an indicator of the reaction in the reaction chamber, and includes DNA, RNA, antigen, antibody, etc. that can be structurally changed such as cleavage or binding depending on the presence of a target material in the sample. The target material may be, for example, DNA, RNA, antigen or antibody derived from a specific pathogen or virus.

For example, when diagnosing malaria infection, an HRP2 or LDH antigen can be used as a target substance, when diagnosing tuberculosis, a CFP-10 antigen can be used, and an antibody against the antigen can be used as a reporter molecule.

For example, when the target substance to be detected is dsDNA, and the target substance is detected using the CRISPR-Cas12a system, the reporter molecule attached to the metal nanoparticles coupled to the end of the optical fiber of the optical fiber sensor member may be ssDNA. In this case, when the polymer of Cas protein and CRISPR RNA specifically binds to and cuts the target DNA having a nucleotide sequence complementary to CRISPR RNA in the sample sample, the non-specific DNase activity of Cas12a is induced, and the metal nanoparticles of the optical fiber sensor are cleaved. The attached ssDNA is cut, and the FO-LSPR equipment detects this change to determine the presence of the target material.

When there are two optical fibers included in the optical fiber sensor member, one strand may be used as a controller to easily determine whether the system operates normally.

For example, when detecting a dsDNA virus using CRISPR-Cas12a, an ssDNA oligomer acting with activated Cas12a is attached to the surface of a metal nanoparticle at the end of one optical fiber, and on the surface of a metal nanoparticle at the end of the other optical fiber. An ssRNA oligomer to which activated Cas13a acts may be attached. Activate Cas13a by designing crRNA corresponding to a molecule that is always present in biological samples (housekeeping genes such as GAPDH and beta-actin) When this is brought into contact with the optical fiber sensor, activated Cas13a cuts the ssRNA oligomer attached to the optical fiber and transmits an LSPR signal, thereby confirming that the system operates normally along with detection of the target material.

In the case of detecting an RNA virus as a target material using CRISPR-Cas13a, the reporter molecule attached to the metal nanoparticles at the ends of the two optical fibers can be configured in the opposite way to that described above.

In one embodiment, in the optical fiber sensor member 31 , the optical fiber 310 passes through the support 311 , and the support serves to support the optical fiber and connect the optical fiber to the adapter 34 at the rear end. When this member is mounted in the corresponding groove 32 of the sensor cartridge so that the optical fiber end is located in the reaction chamber, the exposed optical fiber is placed in the sensor insertion groove 27 (FIG. 4).

In one embodiment, in the optical fiber sensor member 41 , the optical fiber 410 passes through the support 412 , and the support serves to support the optical fiber and connect the optical fiber to the adapter 42 at the rear end. When the optical fiber sensor member is mounted to the cartridge so that the optical fiber end is positioned in the reaction chamber 46, the exposed optical fiber is positioned in the sensor insertion groove 47 (FIG. 7). The optical fiber sensor member may further include a guide 411 for maintaining a distance between the two optical fibers in the cartridge body. When the optical fiber sensor member 41 having two optical fibers is mounted on the cartridge body, the two optical fibers coming out of the guide are arranged to gradually gather toward the sensor chamber 46 .

The optical fiber sensor member 41 and the adapter 42 may be connected after removing the protective cap 413 that protects the other end of the optical fiber passing through the support 412 . The support 412 has a shape or structure that engages with the adapter 42 at the rear end so that it can be easily connected to the adapter.

The support part, the guide, and the protective cap may be made of a general plastic material. for example, The member may be molded from polystyrene, polytetrafluoroethylene, PVC, polypropylene, polyethylene, polycarbonate, polyethylene terephthalate, and the like, but is not limited thereto.

The optical fibers 310 and 410 of the optical fiber sensor members 31 and 41 may use commercially available multimode optical fibers.

The adapter is connected to the optical fiber connection member (eg, SC/PC connector) to connect to the light source and the detector at the rear end. By using such a connection member, the sensor cartridge of the present invention is easily detachable from the optical device.

The disposable sensor cartridge of the present invention may be linked to an LSPR device through an adapter together with an optical fiber sensor mounted therein to construct a molecular diagnostic system, and the system may include a light source, a detector, and a signal analysis program on a computer.

9 exemplarily shows an FO-LSPR device equipped with a sensor cartridge of the present invention. A light source, a detector, and a signal converter are mounted inside the FO-LSPR device body case, and an insertion hole for inserting the sensor cartridge of the present invention is provided outside the case. A signal analysis program is installed on the computer at the top.

The main body case can be manufactured in a size of about 180mm x 90mm x 120mm for on-site diagnosis, and the size can be changed according to purpose or necessity. Correspondingly, the sensor cartridge of the present invention may have a size of, for example, 80 mm x 10 mm x 25 mm, and can be changed according to purposes or needs.

In the FO-LSPR system, the light from the light source is transmitted to the optical fiber. When it is irradiated, it reaches the metal nanoparticles at the end of the optical fiber mounted on the sensor cartridge. The detector detects the returned light and the computer program analyzes it and displays it through a USB voltage measurement module device (eg, commercially available Yocto-milliVolt-Rx, etc.) that converts this analog signal into a digital signal.

Therefore, the optical fiber sensor member of the present invention and the sensor cartridge equipped therewith can detect the presence of the target material with high sensitivity by analyzing the transmission signal of the optical fiber according to the presence or absence of the target material in the sample in conjunction with the FO-LSPR equipment. can

The sensor cartridge of the present invention corresponds to the fluid channel sensor unit of the conventional FO-LSPR, and is basically formed of a semiconductor material such as PDMS on a slide glass in the prior art. The fluid channel sensor unit is integrally configured with the FO-LSPR device, and when it is separated from the optical detection device at the rear end, the optical fiber must be cut. Therefore, it is not only inconvenient to use, but also causes signal stability problems such as changes or errors in optical signals. In addition, mass production was not easy due to the production cost problem due to the use of expensive PDMS material and the production of PDMS material and attaching it on the slide glass. Furthermore, since the sensor is exposed to the outside, it is difficult to carry or transport, so it is limited to laboratory equipment, and there is the complexity of using a syringe pump when injecting or washing a sample into the sensor unit of the fluid channel.

The sensor cartridge of the present invention is molded from general polymer resins such as polystyrene, polytetrafluoroethylene, PVC, polypropylene, polyethylene, polycarbonate, and polyethylene terephthalate, so that the material cost is low and mass production is easy because it can be molded. In addition, it can be easily detached from the optical device at the rear end by using a connecting part such as an adapter, and a sample or washing solution can be injected into the inlet of the sensor cartridge with a simple tool such as a pipette, so it can be used conveniently. In addition, by having a reservoir for storing waste fluid in the cartridge body itself, the waste fluid does not overflow into the external environment when a sample or washing liquid is injected, so it is convenient to operate and suitable for disposable use and disposal .

Furthermore, the fluid channel sensor of the prior art is made of a transparent material and is sensitive to light, so it can be used only in a dark room, whereas the sensor cartridge of the present invention adopts a material and structure that can block light by itself, so that it can be used anywhere indoors or outdoors. have.

One embodiment of the present invention provides a biomolecule diagnostic system using FO-LSPR, including the above-described disposable sensor cartridge and an optical fiber sensor member mounted thereon.

In the biomolecule diagnosis system, the distal end of the optical fiber sensor is exposed to an environment in which a reporter molecule to which metal nanoparticles are attached and a sample exist in a reaction chamber. When the light irradiated from the light source reaches the end of the optical fiber through the connector, the reflected light is received by the detector according to the refractive index according to the reaction state between the ligand attached to the optical fiber and the analyte in the sample, and the detector Converts a quantity to a sensor output value. The presence or absence of a target material may be determined by a change in the intensity value of an output signal of the sensor.

The sensor cartridge of the present invention is equipped with an optical fiber sensor member in which metal nanoparticles with reporter molecules are attached to the end of the optical fiber and is connected to an optical detection device such as FO-LSPR to constitute a biomolecule diagnosis system. In particular, the biomolecular diagnostic system including the optical fiber sensor member and the sensor cartridge of the present invention is convenient for use in small-scale point-of-care diagnostic equipment.

1: sensor cartridge
11: Sample inlet on top of cartridge cover
12: Sample outlet on the top of the cartridge cover
13: Waist inlet on top of cartridge cover
A': cartridge cover
21: Sample inlet on the back of the cartridge cover
22: sample outlet on the back of the cartridge cover
23: Waste inlet on the back of the cartridge cover
24: second fluid passageway
25: second sensor insertion groove
A: Cartridge body
26: reaction chamber
27: first sensor insertion groove
28: first fluid passageway
29: Waste Storage
31: no optical fiber sensor
310: optical fiber
311: support
32, 33: optical fiber sensor member insertion groove
34: adapter
B': cartridge cover
51: sample inlet on the upper surface of the cartridge cover
52: the air outlet on the top of the cartridge cover
55: sample inlet on the back of the cartridge cover
56: Air outlet on the back of the cartridge cover
57: the first protrusion on the back of the cartridge cover
58: second protrusion on the back of the cartridge cover
B: Cartridge body
46: reaction chamber
47: sensor insertion groove
48: fluid passage
49: connecting passage
50: waste storage
41: optical fiber sensor member
410: optical fiber
411: guide
412: support
413: protective cap
42: adapter
Although the embodiments of the present invention have been described above, the present invention is not limited to the above embodiments, but may be implemented in a variety of different forms, and those of ordinary skill in the art to which the present invention pertains will appreciate the technical spirit of the present invention. However, it will be understood that the invention may be embodied in other specific forms without changing essential features. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (10)

A biomolecule diagnostic system using FO-LSPR comprising a disposable sensor cartridge, an optical fiber sensor member mounted thereon, a laser light source, and a photodetector, comprising:
The disposable sensor cartridge includes a cartridge body in which a reaction chamber, a sensor insertion groove, a fluid passage and a waste reservoir are formed, and a cartridge cover in which a sample inlet and a fluid outlet are formed. It is formed symmetrically, and when the optical fiber sensor member is mounted, one end of the optical fiber of the optical fiber sensor member is located in the reaction chamber inside the sensor cartridge, and the other end is located outside the sensor cartridge, and the light source of the FO-LSPR equipment and A biomolecular diagnostic system using FO-LSPR, used in conjunction with a detector. A biomolecule diagnostic system using FO-LSPR comprising a disposable sensor cartridge, an optical fiber sensor member mounted thereon, a laser light source, and a photodetector, comprising:
The disposable sensor cartridge has a cartridge body including a reaction chamber, a first sensor insertion groove, a first fluid passageway and a waste reservoir, and a sample inlet, a sample outlet, and a waste inlet for introducing fluid from the sample outlet into the waste reservoir on the upper surface. is formed, and a cartridge cover having a second fluid passage corresponding to the first fluid passage, a waste inlet, and a second sensor insertion groove corresponding to the first sensor insertion groove formed on the rear surface thereof, the cover and Each component of the body is formed symmetrically in the longitudinal direction of the cartridge, and when the optical fiber sensor member is mounted, one end of the optical fiber of the optical fiber sensor member is located in a reaction chamber inside the sensor cartridge and the other end is located outside the sensor cartridge to connect the optical fiber A biomolecule diagnostic system using FO-LSPR, which is used in conjunction with the light source and detector of the FO-LSPR device through a member. The biomolecule diagnostic system using FO-LSPR according to claim 2, wherein the inflow is caused by forming a portion of the upper surface of the cover including the sample outlet portion as an inclined surface. A biomolecule diagnostic system using FO-LSPR comprising a disposable sensor cartridge, an optical fiber sensor member mounted thereon, a laser light source, and a photodetector, comprising:
The disposable sensor cartridge includes a reaction chamber, a sensor insertion groove, a fluid passageway, and a connection passageway. and a cartridge body having a waste reservoir and a sample inlet and an air outlet formed on the upper surface, and a first protrusion corresponding to the fluid passage and a second protrusion corresponding to the sensor insertion groove on the rear surface of the cartridge It includes a cover, wherein each component of the cover and the body is symmetrically formed in the longitudinal direction of the cartridge, and when the optical fiber sensor member is mounted, one end of the optical fiber of the optical fiber sensor member is located in a reaction chamber inside the sensor cartridge and the other end is A biomolecule diagnosis system using FO-LSPR, which is located outside the sensor cartridge and used in connection with the light source and detector of the FO-LSPR equipment through an optical fiber connection member. [Claim 5] The biomolecule diagnosis system using FO-LSPR according to any one of claims 1, 2, and 4, wherein the cover and the body are foldable or separated from each other. [Claim 5] The biomolecule diagnosis system using FO-LSPR according to any one of claims 1, 2 and 4, wherein a groove in which an optical fiber sensor member is disposed is further formed in the body and/or cover of the cartridge. [Claim 5] The optical fiber sensor member mounted on the disposable sensor cartridge of any one of claims 1, 2, and 4 includes a multimode optical fiber and its support, a biomolecular diagnostic system using FO-LSPR. The FO-LSPR according to claim 7, wherein there is at least one optical fiber included in the optical fiber sensor member, and a DNA oligomer, RNA oligomer, or metal nanoparticle to which an antigen or antibody is attached is bound to the end of the optical fiber. Biomolecular diagnostic system using. The method according to claim 8, wherein when there are two optical fibers included in the optical fiber sensor member, metal nanoparticles having an ssDNA oligomer attached to the end of one optical fiber and metal nanoparticles having an ssRNA oligomer attached to the end of the other optical fiber are Combined, biomolecular diagnostic system using FO-LSPR. delete

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