KR102301576B1 - Cosmetic composition for whitening and wrinkle improvement, comprising a peptide complex obtained from microalgae extract and a ginsenoside complex obtained from a wild ginseng cultured root extract - Google Patents

Abstract

The present invention relates to a method for manufacturing a cosmetic composition for whitening and wrinkle improvement including a peptide complex obtained from a microalgal extract and a ginsenoside complex obtained from a wild ginseng cultured root extract, wherein the method uses a peptide complex manufactured from a plurality of peptides contained in a microalgae extract and a ginsenoside complex obtained from a wild ginseng culture root extract with an increased specific ginsenoside content as main components to exhibit moisturizing, whitening and anti-wrinkle effects.

Description

Cosmetic composition for whitening and wrinkle improvement, comprising a peptide complex obtained from microalgae extract and a ginsenoside complex obtained from a wild ginseng cultured root extract}

The present invention uses a peptide complex prepared from a plurality of peptides contained in a microalgae extract and a ginsenoside complex obtained from a wild ginseng culture root extract with an increased specific ginsenoside content as main components to show moisturizing, whitening and anti-wrinkle effects. It relates to a cosmetic composition for whitening and wrinkle improvement comprising a peptide complex obtained from a microalgae extract and a ginsenoside complex.

Human skin is composed of the epidermis, dermis, and skin tissue, and the dermal layer below the epidermis is composed of collagen, elastin, and hyaluronic acid. In particular, collagen is a connective tissue composed of amino acids, a type of protein, and as human aging progresses, the amount of dense collagen fibers decreases, and as it loosens, elasticity decreases, causing the skin to sag or deep wrinkles to make the face look bumpy and wide. do. Of course, aging is a phenomenon common to all humans over time, but aging is a disaster for many people who value beauty.

Methods for inhibiting aging are constantly being studied, and in particular, methods for increasing the collagen are also being studied together. There are also various ingredients that are attracting attention in anti-aging research. In particular, even in the case of peptides, which are substances made by combining two or more amino acids, it is a component attracting attention in that it can directly supplement collagen in the dermal layer. In the case of peptides, since the particles are smaller than the protein form, it has the advantage of penetrating deep into the dermis layer, so a better skin improvement effect can be expected.

For reference, in the case of Korean Patent Laid-Open Publication No. 10-2017-0003903, a cosmetic composition excellent in anti-aging or anti-wrinkle effect through the complementary action of vitamins and peptides was provided. However, in the case of niacinamide (vitamin B) used in the above patent, when more than a certain component is included, it may precipitate in the composition to reduce the stability of external preparations, and ascorbyl glucoside (vitamin C) as a vitamin C derivative There was a problem that the activity was weak.

Korean Patent No. 10-1803283

The present invention is to solve the above problems, and a peptide complex prepared by mixing each peptide obtained from microalgae, and a ginsenoside complex obtained from a wild ginseng cultured root extract with an increased specific ginsenoside content as main components. An object of the present invention is to provide a method for preparing a cosmetic composition comprising a peptide complex obtained from a microalgae extract and a ginsenoside complex obtained from a wild ginseng cultured root extract, which can exhibit moisturizing, whitening and wrinkle improvement effects.

As a means for solving the above problems, the configuration of the present invention is 0.5 to 10.6 parts by weight of a peptide complex, 0.001 to 15 parts by weight of a ginsenoside complex, 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of a moisturizer, 0.001 to ceramide 10 parts by weight and 0.01 to 2 parts by weight of a preservative.

The peptide complex is, Chlorella vulgaris extract, Dunaliella salina extract, Schizochytrium aggregatum extract, Schizochytrium limacinum extract, Schizochytrium limacinum extract, Schizochytrium Minuteum (Schizochytrium minutum) extract, Cryptodinium cohnii (Crypthecodinium cohnii) extract and Haematococus pluviallis (Haematococus pluviallis) It is characterized in that the peptide obtained from each of the extract is a mixed peptide complex.

The ginsenoside complex is obtained by obtaining ginsenosides Rg1 and Rg2 from any one of wild ginseng cultured root extract, red ginseng extract, ginseng extract, black ginseng extract or old ginseng extract, each of which is subjected to high-pressure enzymatic decomposition after steaming twice. Ginsenosides Rg1 and Rg2 are each 1-3: characterized in that it is mixed in a weight ratio of 1-3.

The present invention uses a peptide complex prepared from a plurality of peptides contained in a microalgae extract and a ginsenoside complex obtained from a wild ginseng cultured root extract with increased specific ginsenoside content as main components to prevent aging, moisturizing, whitening and wrinkle improvement. effect can be shown.

Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings in order to describe in detail enough that a person of ordinary skill in the art can easily carry out this invention. Other objects, including the object, action, effect, and operational advantages of this invention will become clearer by the description of the preferred embodiment.

For reference, the embodiments disclosed herein are only presented by selecting and presenting the most preferred embodiments to help those skilled in the art from among various possible implementations, and the technical spirit of the present invention is not necessarily limited or limited only by the presented embodiments. No, various changes, additions, and modifications including equivalents to substitutes are possible within the scope not departing from the technical spirit of the present invention.

In addition, the terms or expressions used in the specification and claims are defined on the basis of the principle that the inventor can appropriately define the concept of a term in order to best describe his invention, and It should not be construed as being limited to a dictionary meaning, but should be interpreted as meaning and concept consistent with the technical idea of this invention. As an example, the singular expression includes the plural expression unless the context clearly dictates otherwise.

The cosmetic composition for whitening and wrinkle improvement, comprising the peptide complex obtained from the microalgae extract and the ginsenoside complex obtained from the wild ginseng cultured root extract according to the present invention, is a peptide complex 0.5 to 10.6 parts by weight, ginsenoside complex 0.001 to 15 It contains 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of a moisturizer, 0.001 to 10 parts by weight of ceramide, and 0.01 to 2 parts by weight of a preservative.

The peptide complex is, Chlorella vulgaris extract, Dunaliella salina extract, Schizochytrium aggregatum extract, Schizochytrium limacinum extract, Schizochytrium limacinum extract, Schizochytrium Minuteum (Schizochytrium minutum) extract, Crypthecodinium cohnii extract, and each peptide obtained from Haematococus pluviallis extract is characterized in that it is a mixed peptide complex.

Schizochytrium aggregatum, Schizochytrium limacinum and Schizochytrium minutum are Schizochytrium sp. and are used for DNA production and are used for creep Thecodinium cohnii is also used for DNA production.

Haematococus pluviallis (Haematococus pluviallis) is a species of Haematococus (Haematococus sp.), which is known to produce astaxanthin, an antioxidant component, and is known to be effective in improving blood circulation.

It is known that the peptides extracted from these 7 types of microalgae act on nerve cells to induce activity, and induce activation of skin tissue through various signal transduction processes.

Ginsenoside is a type of saponin, a type of glycoside. It is derived from the roots, stems, leaves, bark, seeds of plants and is also contained in the body of some echinoderms (starfish, sea cucumber). have. Saponin is known as a component that enhances the immune system of the human body, and ginsenoside, a type of saponin contained in ginseng, red ginseng, wild ginseng, old ginseng, and black ginseng, is particularly famous.

In particular, rare ginsenoside is a word that refers to saponin contained only in ginseng and is used to distinguish it from saponins of other plants. Rare ginsenosides have a smaller material size than general ginsenosides and have structural characteristics that are relatively easy to absorb into the body. In addition, when regular ginsenosides are consumed, a small amount is converted to rare ginsenosides by the intestinal microflora and absorbed, but because there is a large individual difference, taking the rare ginsenoside form is more efficient in terms of absorption and bioavailability in the body. have.

Ginsenosides include ginsenosides Rg1, Rg2, Rg3, Rg4. Various types such as Rg6, Rb1, Rb2, Rh1, Rh2, Rc, Rd, Re, and Ro exist, different types and amounts.

Ginsenoside Rg1 is known as a substance that strengthens immune function and acts as an anti-fatigue, and Rg2 is known as a substance that inhibits platelet aggregation.

The ginsenoside complex according to the present invention is obtained by obtaining ginsenosides Rg1 and Rg2 from any one of wild ginseng cultured root, red ginseng, ginseng, black ginseng or old ginseng, each of which has been subjected to high-pressure enzymatic decomposition after steaming twice. The senoside Rg1 and Rg2 are each 1-3: characterized in that it is made by mixing in a weight ratio of 1-3.

Hereinafter, a method for preparing such a peptide complex and a ginsenoside complex will be described.

(Preparation of peptide complexes)

Chlorella vulgaris (Chlorella vulgaris), Dunaliella salina (Dunaliella salina), Schizochytrium aggregatum (Schizochytrium aggregatum), Schizochytrium limacinum (Schizochytrium limacinum), Schizochytrium minutum (7 species of microalgae) Schizochytrium minutum), Cryptodinium cohnii, and Haematococus pluviallis were subcultured in flasks for 10-30 days by preparing culture conditions and medium suitable for each growth temperature and pH. did.

In the incubator for culture, a light source was installed to facilitate photosynthesis to create a culture environment, and in order to maintain the concentration of carbon dioxide in the culture medium at an appropriate level, flask culture was performed using a carbon dioxide incubator.

Thereafter, the microalgae after culturing are centrifuged at 5,000 to 10,000 rpm for 20 to 30 minutes respectively to remove moisture first, and then freeze-drying the microalgae from which the moisture has been removed to some extent through a centrifuge to secondarily remove the remaining moisture. did.

Methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof, an organic solvent, is used, and the active ingredient of the herbal medicine is not destroyed or can be extracted by heating at room temperature or under conditions that are minimized. Depending on the organic solvent to be extracted, the degree of extraction and loss of the active ingredient of the drug may be different, so an appropriate organic solvent should be selected and used. In addition, it can be obtained through various known extraction methods not specifically mentioned herein, for example, physical methods, hot water extraction, ethanol extraction, and the like.

To extract the peptide absorbed in the organic solvent, it was put into a separator having a filtration membrane suitable for the size of the peptide to obtain only the peptide, and a peptide complex was prepared by mixing 7 peptides extracted from each microalgal extract.

Specifically, the peptide complex according to the present invention, the first peptide obtained from the Chlorella vulgaris extract, the second peptide obtained from the Dunaliella salina extract, Schizochytrium aggregatum ) the third peptide obtained from the extract, the fourth peptide obtained from the Schizochytrium limacinum extract, the fifth peptide obtained from the Schizochytrium minutum extract, Cryptocodinium cony ( Crypthecodinium cohnii) extract and the sixth peptide obtained from the extract and the seventh peptide obtained from the Haematococus pluviallis extract, respectively, 1: 2: 2: 1: 1: 1: 2: 2 in a weight ratio of 2 to a stirrer After adding, it can be obtained by stirring at 500 to 1000 rpm for 5 to 8 hours.

(Preparation of ginsenoside complex)

First, 5 kg of cultured wild ginseng root was crushed and subjected to a primary steaming treatment for 8 hours at a temperature of 30° C. and a humidity of 40%. Next, the first steam-treated wild ginseng cultured root was placed in a freeze dryer and first dried at -50°C for 10 hours. Then, the primary dried wild ginseng cultured roots were subjected to a secondary steaming treatment for 8 hours at a temperature of 30° C. and a humidity of 40%. Next, the second steam-treated wild ginseng cultured root was placed in a freeze dryer and secondarily dried at -50°C for 10 hours.

A natural product powder was prepared by pulverizing a sample dried with a freeze dryer to a size of 60 to 120 mesh, respectively. In addition, the citric acid aqueous solution for use as an extraction solvent was prepared by diluting with distilled water until the pH of the 0.2M citric acid solution was 4.8. Then, 1 part by weight of natural product powder (cultivated wild ginseng powder) was added to 100 parts by weight of the prepared pH 4.8 citric acid aqueous solution to prepare an extraction mixture (sample 30 mg/citric acid solution 30 mL). Based on 30 mL of the above extraction mixture, 50 to 100 μL of Viscozyme (KTN02214) or Pectinex Ultra SP-L (KRN05658) from Novozymes (Bagsvaerd, Denmark), or a mixture of two enzymes (Viscozyme + Pectinex) is added to the extraction mixture.

The extract mixture prepared in this way was administered to a polypropylene bag (Nasco, WHIRL-PAK-4oz.), which is a plastic container for ultra-high pressure reactor, and sealed so that there are no air bubbles, and a sample for high-pressure enzyme reaction treatment was prepared. A high-pressure reactor dedicated container containing the extract mixture containing the enzyme is placed in a high-pressure reactor (Toyo Koatsu, TFS-2L), and the treatment conditions of the high-pressure reactor are set to a pressure of 100 MPa, a temperature to 50° C., and a high pressure treatment time to 8 hours. was operated. After 8 hours, the extraction mixture was recovered, and a sample was prepared in which 45 mL of ethanol was added based on 30 mL of the extraction mixture. The comparative group was used as a wild ginseng root extract solution extracted by immersion in a water-bath at 45° C. under atmospheric pressure (0.1 MPa) for 4 hours.

In order to detect the type and content of ginsenosides contained in the experimental group, which is a sample prepared by the above method and ultra-high pressure, and the comparative group, which is a sample subjected to atmospheric pressure, the indicator components of each sample were analyzed according to the following method. .

analysis procedure

1. Preparation of test solution

1) Accurately weigh about 1 g of the sample, put it in a 250 mL reflux flask, add 50 mL of 50% methanol solution, extract under reflux in a water bath at 70~80oC for 1 hour, cool, centrifuge, and take the supernatant (supernatant A) .

2) Add 50 mL of 50% methanol solution to the residue again, extract under reflux for 1 hour in a water bath at 70~80oC, cool and centrifuge to obtain a supernatant (supernatant B).

3) Combine supernatant solution A and supernatant solution B together, put it in a 250 mL concentration flask, and concentrate under reduced pressure at 60°C or less.

4) The concentrate is dissolved in 2 mL of a water-acetonitrile (80:20 V/V) mixed solvent, filtered through a 0.45 μm membrane filter, and used as a test solution for HPLC analysis.

2. HPLC analysis

20 μL of test solution and 20 μL of standard solution are injected into HPLC and analyzed according to the following conditions to obtain the peak area of each ginsenoside.

1) Column: ODS (4.6 mm × 250 mm) or equivalent or higher

2) Detector: UV (measurement wavelength: 203 nm)

3) Mobile phase: water-acetonitrile mixed solvent (flow: 1.6 mL/min)

3) Calculation of ginsenoside content

The amount of each ginsenoside contained in the sample is calculated according to the following formula.

[Equation 1]

Y=S(a*b) / A*0.001

Here, Y is the ginsenoside content, S is the concentration of individual ginsenosides in the test solution (μg/mL), a is the total amount of the test solution (mL), b is the dilution factor, and A is the sample amount (g). do.

The total content of ginsenosides of the enzyme-treated and ultra-high pressure-treated sample calculated in this way was 28.34 mg/g, which increased by 22.4% compared to the ginsenoside content (23.15 mg/g) of the atmospheric pressure-treated sample (comparative group) did

In the same way as above, ginsenosides Rg1 and Rg2 were obtained from wild ginseng cultured roots, respectively, and then ginsenosides Rg1 and Rg2 were added to a stirrer in a weight ratio of 1-3: 1-3, respectively, and 500 to 1000 rpm for 5 hours. A ginsenoside complex was prepared by stirring for 8 hours.

(Preparation of a cosmetic composition comprising a peptide complex and a ginsenoside complex)

0.5 to 10.6 parts by weight of a peptide complex, 0.001 to 15 parts by weight of a ginsenoside complex, 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of a moisturizer, 0.001 to 10 parts by weight of a ceramide, and 0.01 to 2 parts by weight of a preservative. prepared.

The composition of the present invention preferably contains the peptide complex in an amount of 0.5 to 10.6 parts by weight based on the total weight. If the content of the peptide complex is less than 0.5 parts by weight, there is a problem in that the melanin production inverse effect is lowered, and if it exceeds 10.6 parts by weight, problems in skin safety or formulation may occur.

In the present invention, it is preferable to contain the ginsenoside complex in an amount of 0.001 to 15 parts by weight based on the total weight. If the content of the ginsenoside complex is less than 0.001 parts by weight, the efficacy and effect of the above ingredients are weak, and if it exceeds 15 parts by weight, problems with skin safety or formulation may occur.

The ceramide is a substance belonging to the lipid, and is the most important component among the oils and fats contained in the keratin of the skin, and it is said that it plays a large role in forming a skin protective film that acts against the external environment, and also has the function of strengthening the skin lipid barrier. it is known that there is

On the other hand, the cosmetic composition containing the peptide complex derived from the microalgal extract of the present invention is not particularly limited in its formulation, for example, toner, lotion, lotion, gel, water-soluble liquid, cream, essence, mist, water. It may have formulations such as heavy oil (O/W) type and water-in-oil (W/O) type. And, in the cosmetic composition of each formulation, other components other than the peptide complex derived from the microalgal extract can be selected and formulated without difficulty by those skilled in the art according to the formulation or purpose of use of other cosmetics.

A cosmetic composition was prepared in the composition ratio shown in Table 1 below using the peptide complex, the ginsenoside complex and other auxiliary ingredients according to the present invention.

(Experimental Example 1) Skin improvement effect

In order to confirm the skin improvement effect of the cosmetic composition according to the present invention, a cosmetic composition (Examples 1 to 4, Comparative Examples 1 to 4) was prepared, and a skin improvement evaluation experiment was performed on a total of 22 subjects in their 20s and 50s. did. The subjects were divided into 8 groups (Examples 1 to 4, Comparative Examples 1 to 4), and used once a day for 8 weeks.

First, the moisturizing effect was calculated according to Adolf Fick's Law by calculating the amount of moisture emitted from the skin using a Tewameter. The amount of change before and after using the cosmetic composition was quantified.

Next, the whitening and wrinkle improvement effects were evaluated by writing the degree of improvement as a score according to the opinions of the subjects.

Subsequently, the effect of inhibiting melanin production was confirmed by measuring the melanin biosynthesis of zlatin peptide compound in skin melanocytes.

The cut skin tissue was washed with a phosphate buffer solution (PhosphateBufferedSaline) containing antibiotics (50mg/ml gentamicin, 50mg/ml amphotericin, 50U/ml penicillin, 50㎍/streptomycin) and treated with 0.25% Trypsin/EDTA for 5 minutes. ^{After culturing for 15 days in an incubator at 5% CO 2} and 37° C. in M2 medium (Promocell), a melanocyte selective medium, the melanocyte cells are removed again with 0.25% Trypsin/EDTA, and the number of cells is counted with a hemocytometer. ^{Inoculate 1×10 4} cells per well in 24-well containing medium (Promocell) and incubate at 37°C until the cells adhere to the well at least 80%d. After culturing for 1 day, dopa, a precursor of melanin pigment, was added so as to be 0.08 mM, and then the gelatin peptide compound was added at a test concentration of 0.5 wt%, respectively, and cultured for 3 days, and saline was added to the control group. After removing the medium, the cells are washed with a phosphate buffer solution (PBS, PhosphateBufferedSaline) and treated with trypsin to recover the cells. After measuring the number of cells using a hematocytometer, the recovered cells are centrifuged at 5,000 to 10,000 for 10 minutes, and then the supernatant is removed to obtain a precipitate. Add 100 μl of 1M sodium hydroxide solution and obtain intracellular melanin in a thermostat at 60°C. With this solution, absorbance was measured at 490 with a microplate, and the melanin content per cell count was expressed as % by comparing the control group not treated with the gelatin peptide compound and the experimental group. The results are shown in Table 2 below.

+++: Very good improvement effect

++: Significant improvement

+: There is a slight improvement effect

±: No improvement effect, but no deterioration

-: No improvement effect, rather worse

As described above, in the case of the subjects using Examples 1 to 4, excellent moisturizing effect was exhibited through high moisture content, and the skin improvement was evaluated as excellent. The subjects who used the cosmetic compositions of Comparative Examples 1 to 3 showed an evaluation that they did not noticeably feel the degree of skin improvement, and in Comparative Example 4, the degree of skin improvement was found to be similar to that of Examples.

In addition, in Comparative Example 1 in which the peptide complex was not included in the melanin inhibitory effect experiment, the only melanin content was significantly higher. In particular, in the case of Example 4 containing the peptide complex and the ginsenoside complex, respectively, it was significantly reduced, which increased the skin absorption rate when the content of the peptide complex and the ginsenoside complex was mixed in a preferred ratio according to the present invention is considered to have been Therefore, the cosmetic composition of the present invention exhibits a skin improvement effect.

(Comparison of efficacy according to the mixing ratio of the microalgae-derived peptide complex)

In order to compare the skin improvement effect of the peptide complex according to the present invention in more depth, the efficacy according to the mixing ratio of the peptide complex derived from microalgae was compared. Specifically, the peptide complex according to the present invention, the first peptide obtained from the Chlorella vulgaris extract, the second peptide obtained from the Dunaliella salina extract, Schizochytrium aggregatum ) the third peptide obtained from the extract, the fourth peptide obtained from the Schizochytrium limacinum extract, the fifth peptide obtained from the Schizochytrium minutum extract, Cryptocodinium cony ( Crypthecodinium cohnii) the sixth peptide obtained from the extract and the seventh peptide obtained from the Haematococus pluviallis extract, respectively 1: 2: 2: 1: 1: 1: 2: A mixture of 2 by weight ratio A cosmetic composition was prepared in Examples, and a cosmetic composition comprising a peptide complex mixed in a ratio different from the above-mentioned ratio was prepared in Comparative Examples (see Table 3).

Thereafter, a total of 50 adult males and females between 20 and 50 years of age were divided into five groups of 10 each, and each cosmetic composition was applied to a designated test site for 2 weeks to determine the degree of skin improvement. The results are shown in Table 4 below.

+++: Very good improvement effect

++: Significant improvement

+: There is a slight improvement effect

±: No improvement effect, but no deterioration

-: No improvement effect, rather worse

As shown in the above results, the subjects using Comparative Examples 1 to 4 showed an evaluation that they did not noticeably feel the skin improvement effect, and the subjects using the Examples evaluated that the skin improvement effect was generally excellent. Therefore, it can be seen that the cosmetic composition according to the present invention has excellent skin improvement effect when each peptide is mixed in the ratio according to the embodiment.

(Comparison of the effect of inhibiting melanin production according to the components of the ginsenoside complex)

On the other hand, in order to compare the skin improvement effect improvement performance of the ginsenoside complex according to the present invention in more depth, according to the present invention, the ginsenoside Rg1 and Rg2 are each mixed in a weight ratio of 1: 2 containing a complex A cosmetic composition was prepared as an experimental example, and a cosmetic composition containing a complex in which ginsenosides Rg1 and Rg2 were mixed in a weight ratio of 1: 1 was used as Comparative Example 1, and ginsenosides Rg1 and Rg2 were each 1:3. A cosmetic composition containing a composite mixed in a weight ratio is Comparative Example 2, and a cosmetic composition containing a composite in which ginsenosides Rg1 and Rg2 are each mixed in a weight ratio of 2:1 is Comparative Example 3, ginsenoside A cosmetic composition containing a compound in which the sides Rg1 and Rg2 are mixed in a weight ratio of 3:1, respectively, was prepared as Comparative Example 4, and a cosmetic composition containing a general red ginseng extract was prepared as Comparative Example 5.

Thereafter, the melanogenesis inhibitory function was assayed at the cellular level through B16 melanoma cell culture. B16 melanoma cells were cultured in DMEM (Dulbecco's modified Eagle's Medium) containing 10% bovine serum at 5% CO ₂ , 37 ° C. for 2 days until 1.0 X 10 ⁸ cells/T25 flask. Then, the samples according to Examples and Comparative Examples 1-5 were administered to the cells and _{cultured at 5% CO 2} , 37° C. for 2 days.

Then, trypsin was added to collect the cells, and the degree of blackening of the cells was observed. Take the supernatant of the T25 flask from which melanin is generated and discharged, measure the absorbance at 475 nm to quantify melanin, and the number of cells is the number of viable cells by mixing the collected cells: tryphan blue = 1: 9 in a ratio and observing it under a microscope. Count and quantify the amount of melanin produced per unit cell to indicate the inhibitory effect.

As described above, it can be seen that Examples and Comparative Examples 1-4 containing ginsenoside Rg1 and Rg2 had a higher melanin production inhibition rate than Comparative Example 5 containing only general red ginseng extract, and in particular, ginsenoside Rg1, Rg2 It can be seen that the inhibition rate of melanin production of the Examples each is mixed in a weight ratio of 1: 2 is significantly higher.

Therefore, a cosmetic for whitening and wrinkle improvement comprising a specific ginsenoside complex in which the peptide complex obtained from the microalgae extract according to the present invention and the ginsenosides Rg1 and Rg2 are mixed in a weight ratio of 1-3: 1-3, respectively It can be seen that the composition has an excellent skin improvement effect.

Although the embodiments of the present invention have been described above, those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential features thereof. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (3)

0.5 to 10.6 parts by weight of the peptide complex, 0.001 to 15 parts by weight of the ginsenoside complex, 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of a moisturizer, 0.001 to 10 parts by weight of ceramide and 0.01 to 2 parts by weight of a preservative,
The peptide complex is
The first peptide obtained from the Chlorella vulgaris extract, the second peptide obtained from the Dunaliella salina extract, the third peptide obtained from the Schizochytrium aggregatum extract, Schizokit A fourth peptide obtained from an extract of Schizochytrium limacinum, a fifth peptide obtained from an extract of Schizochytrium minutum, a sixth peptide obtained from an extract of Crypthecodinium cohnii, and The seventh peptide obtained from the Haematococus pluviallis extract is each 1: 2: 2: 1: 1: 1: characterized in that it is mixed in a weight ratio of 2: 2,
The ginsenoside complex is
Ginsenosides Rg1 and Rg2 were obtained from any one of wild ginseng cultured root, red ginseng, ginseng, black ginseng or old ginseng, which was subjected to high-pressure enzymatic decomposition after steaming twice, and the obtained ginsenosides Rg1 and Rg2 were respectively 1-3: A cosmetic composition for whitening and wrinkle improvement comprising a peptide complex obtained from a microalgae extract, characterized in that it is mixed in a weight ratio of 1 to 3.

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