KR102287039B1 - Functional cereals cultured with mycelium extract of mushrooms and method for manufacturing the same - Google Patents
Functional cereals cultured with mycelium extract of mushrooms and method for manufacturing the same Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B2/00—Preservation of foods or foodstuffs, in general
- A23B2/10—Preservation of foods or foodstuffs, in general by treatment with pressure variation, shock, acceleration or shear stress
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B2/00—Preservation of foods or foodstuffs, in general
- A23B2/40—Preservation of foods or foodstuffs, in general by heating loose unpacked materials
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- A23L3/015—
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- A23L3/16—
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
- A23L31/10—Yeasts or derivatives thereof
- A23L31/15—Extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
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- Polymers & Plastics (AREA)
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- Microbiology (AREA)
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Abstract
본 발명은 버섯류의 균사체 추출물로 배양한 기능성 곡류 및 그 제조방법에 관한 것이다.
본 발명은 이를 위해 이물질을 걸러낸 곡류를 깨끗히 수세하고, 물에 침지시키는 단계; 곡류를 채반 그릇에 올려 놓고 물기를 제거하는 단계; 물기가 제거된 곡류를 병이나 비닐봉지에 입봉한 후 필터가 있는 마개를 닫아 장착하는 단계; 병이나 비닐봉지의 마개를 닫아 밀봉한 후 곡류를 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계; 멸균이 끝난 곡류를 자연 배기시켜 하온시키는 단계; 자연 배기된 곡류에 약용버섯 액체 종균 배지 조성물을 접종한 후 곡류로 약용버섯 균사체를 배양하는 단계; 및 액체 종균이 접종된 곡류의 뭉친 부분을 풀어주고, 열풍으로 건조하는 단계;가 포함됨을 특징으로 하는 버섯류의 균사체 추출물로 배양한 기능성 곡류 제조방법을 제공한다.
상기와 같이 구성된 본 발명은 약용버섯(예: 상황버섯, 동충하초 등)을 곡류(예: 쌀·현미·밀·보리·옥수수 등의 곡식 등)에 액체 종균으로 스며들게 접종할 수 있도록 하여 배양 일수를 현격히 감소시키면서 동시다발적으로 고른 배양을 유도할 수 있고, 이로 인해 기능성 곡류의 품질과 신뢰성을 대폭 향상시키므로 사용자인 소비자들의 다양한 욕구(니즈)를 충족시켜 좋은 이미지를 심어줄 수 있도록 한 것이다.The present invention relates to functional grains cultured with a mycelium extract of mushrooms and a method for producing the same.
For this purpose, the present invention includes the steps of washing the grains filtered out of foreign substances with water and immersing them in water; placing the grains on a sieve bowl and draining the water; After sealing the water-removed grains into a bottle or a plastic bag, closing the cap with a filter and mounting; High-pressure sterilization (sterilization) and sterilization (sterilization) of the grain after sealing the bottle or plastic bag by closing the stopper; heating the sterilized grains by naturally evacuating them; culturing medicinal mushroom mycelium with cereals after inoculating the medicinal mushroom liquid seed culture medium composition into naturally evacuated cereals; And it provides a method for producing functional grains cultured with the mycelium extract of mushrooms, characterized in that it;
The present invention configured as described above allows the inoculation of medicinal mushrooms (eg, Sanghwang mushroom, Cordyceps, etc.) to grains (eg, rice, brown rice, wheat, barley, corn, etc.) as a liquid seed so that the inoculation can be performed to reduce the number of inoculation days. It is possible to induce a simultaneous and even culture while significantly reducing, thereby greatly improving the quality and reliability of functional grains.
Description
본 발명의 실시예는 버섯류의 균사체 추출물로 배양한 기능성 곡류 및 그 제조방법에 관한 것으로, 보다 상세하게는 약용버섯(예: 상황버섯, 동충하초 등)을 곡류(예: 쌀·현미·밀·보리·옥수수 등의 곡식 등)에 액체 종균으로 스며들게 접종할 수 있도록 하여 배양 일수를 현격히 감소시키면서 동시다발적으로 고른 배양을 유도할 수 있고, 이로 인해 기능성 곡류의 품질과 신뢰성을 대폭 향상시키므로 사용자인 소비자들의 다양한 욕구(니즈)를 충족시켜 좋은 이미지를 심어줄 수 있도록 한 것이다.An embodiment of the present invention relates to functional cereals cultured with a mycelium extract of mushrooms and a method for producing the same, and more particularly, medicinal mushrooms (eg, Sanghwang mushroom, cordyceps, etc.) It is possible to inoculate grains such as corn, etc.) with a liquid starter, so that the number of culture days can be significantly reduced and simultaneous and even culture can be induced. It is designed to satisfy the various needs (needs) of the people and to instill a good image.
주지하다시피 버섯류(fungi음성듣기, 茸類)는 담자균류에 속하는 고등균류의 총칭이다.As is well known, mushrooms (fungi hearing, 茸類) is a generic term for higher fungi belonging to the basidiomycetes.
버섯은 균류(菌類, fungi)의 속칭으로 번식을 위해서 만든 포자(胞子)의 생성방법에 따라 담자균류(擔子菌類)와 자낭균류(子囊菌類)로 분류되는데, 대부분은 담자균류에 속한다. 버섯의 식용에 쓰이는 부분은 자실체 부분이다. 버섯의 종류는 수천 종으로 현재 식용되고 있는 것은 약 180여 종이 넘는다. 식용버섯, 약용버섯, 독버섯의 세 가지로 나눈다. 재배할 수 없는 천연버섯에는 송이버섯, 밤버섯, 싸리버섯, 송로(松露) 등, 재배버섯에는 표고버섯, 느타리버섯, 목이버섯, 양송이버섯 등, 약용버섯에는 영지버섯 등이 있다. 일반성분은 채소류와 흡사하며, 프로비타민 D2인 에르고스테롤(ergosterol)의 함량이 많은 것이 특징이다. 버섯류에는 효소가 많으므로 변질되기 쉽다. 따라서 건조하거나 염장한다. 버섯류는 독특한 풍미(향기·맛)가 있는 기호성이 강한 식품이다. 또 표고버섯, 양송이 등에는 특수한 생리효과가 있는 성분이 포함되어 있다.Mushrooms are a generic name for fungi, and are classified into basidiomycetes and ascomycetes according to the production method of spores made for propagation, and most of them belong to basidiomycetes. The edible part of the mushroom is the fruiting body part. There are thousands of types of mushrooms, and more than 180 of them are currently edible. It is divided into three types: edible mushrooms, medicinal mushrooms, and poisonous mushrooms. Natural mushrooms that cannot be cultivated include pine mushrooms, chestnuts, shiitake mushrooms, and truffles, cultivated mushrooms include shiitake, oyster mushrooms, oyster mushrooms, and oyster mushrooms, and medicinal mushrooms include reishi mushrooms. The general ingredients are similar to vegetables, and it is characterized by a high content of ergosterol, a provitamin D2. Mushrooms have a lot of enzymes, so they are easy to change. Therefore, it is dried or salted. Mushrooms are highly palatable foods with a unique flavor (scent and taste). In addition, shiitake mushrooms and mushrooms contain ingredients with special physiological effects.
특히 버섯은 고단백 식품이며 각종 연구보고에 의하여 항암작용을 하는 것으로 알려져 있다. In particular, mushrooms are a high-protein food and are known to have anticancer effects according to various research reports.
이러한 항암작용이 있는 버섯류로는 영지버섯과 상황버섯을 들 수 있는 데, 영지버섯은 예로부터 불로초로 일컬을 만큼 약효가 있고 희귀한 것이었으나 오늘날에는 원목재배법, 단목재배법, 모래재배법 등과 같은 인공적인 대량 재배기술의 개발로 인하여 대량 생산됨에 따라 각종 건강식품으로 사용되어 잘 알려져 있으나, 이에 반해 상황버섯은 원래 뽕나무의 고목에서만 자생하기 때문에 일반인들에게 잘 알려지지 않았으며 그 재배 방법도 크게 연구되지 않아 주목받지 못하였으나 최근에 들어서 어느 버섯류보다도 항암효과가 높다는 사실이 발표되면서 인공재배 방법도 많이 연구되는 등 많은 관심을 가지게 되었다.Mushrooms with such anti-cancer action include reishi mushroom and sanghao mushroom. Reishi mushroom has medicinal effects and is rare enough to be called bullocho from the old days. As it is mass-produced due to the development of cultivation technology, it is well known for being used as a variety of health food. On the other hand, Sanghwang mushroom is not well known to the general public because it grows only in the old mulberry tree, and its cultivation method has not been studied much, so it is not well-known. However, recently, as it was announced that it has a higher anticancer effect than any other mushroom, artificial cultivation methods have been studied a lot, and a lot of interest has been drawn.
종래 버섯류를 이용하여 균사체를 쌀에 배양하는 기술이 있으나, 고체 성분의 균사체를 쌀 표면에 엷은 막을 덮어 씌워는 작업이 어렵고, 또한 시간이 오래 걸려 그 효용 가치가 높지 않았다.There is a conventional technique for culturing the mycelium on rice using mushrooms, but it is difficult to cover the mycelium of a solid component on the surface of the rice with a thin film, and it takes a long time, so the utility value was not high.
상기한 문제점을 해결하기 위해 종래에는 아래와 같은 선행기술문헌들이 개발되었으나, 여전히 상기한 종래 기술의 문제점을 일거에 해결하지 못하는 커다란 문제점이 발생 되었다.In order to solve the above problems, the following prior art documents have been developed in the prior art, but there is still a big problem in that the problems of the prior art cannot be solved at once.
본 발명은 상기와 같은 종래 기술의 제반 문제점을 해소하기 위하여 안출한 것으로, 물에 침지시키는 단계, 물기를 제거하는 단계, 필터가 있는 마개를 닫아 장착하는 단계, 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계, 자연 배기시켜 하온시키는 단계, 곡류로 약용버섯 균사체를 배양하는 단계 및 뭉친 부분을 풀어주고, 열풍으로 건조하는 단계가 포함됨을 제1목적으로 한 것이고, 상기한 기술적 구성에 의한 본 발명의 제2목적은 약용버섯(예: 상황버섯, 동충하초 등)을 곡류(예: 쌀·현미·밀·보리·옥수수 등의 곡식 등)에 액체 종균으로 스며들게 접종할 수 있도록 한 것이고, 제3목적은 액체 종균을 사용함으로 인해 배양 일수를 종래에 비해 현격히 감소시킬 수 있도록 한 것이고, 제4목적은 액체 종균이 곡류에 고르게 스며들기 때문에 수확량을 현저하게 증가시킬 수 있도록 한 것이고, 제5목적은 액체 종균이 동시다발적으로 곡류에 골고루 스며들기 때문에 고른 배양을 유도할 수 있도록 한 것이고, 제6목적은 약용버섯이 함유된 기능성 곡류를 소비자들이 일상적인 식사 과정을 통해 손쉽게 섭취함으로 건강 증진 특히 면연력을 높일 수 있도록 한 것이고, 제7목적은 단시간 내에 약용버섯이 함유된 고품질의 곡류를 생산하므로 소비자들에게 기능성 곡류를 보다 싼 값으로 공급할 수 있도록 한 것이고, 제8목적은 버섯이 가지는 약리 효과와 곡류가 가지는 독특한 효능 및 이용성 그리고 편의성의 상승 효과를 기대할 수 있도록 한 것이고, 제9목적은 버섯이 가지는 약리 효과로 인해 각종 암을 예방하고 종양을 없애줄 수 있고 질병에 대하여 예방 및 억제할 수 있도록 한 것이고, 제10목적은 재배농가의 소득을 증대시키고 인류 건강에 크게 이바지할 수 있도록 한 것이고, 제11목적은 이로 인해 기능성 곡류의 품질과 신뢰성을 대폭 향상시키므로 사용자인 소비자들의 다양한 욕구(니즈)를 충족시켜 좋은 이미지를 심어줄 수 있도록 한 버섯류의 균사체 추출물로 배양한 기능성 곡류 및 그 제조방법을 제공한다.The present invention has been devised in order to solve all the problems of the prior art as described above, a step of immersing in water, a step of removing water, a step of closing and mounting a cap with a filter, high-pressure sterilization (sterilization) and sterilization (sterilization) ) treatment, natural exhaust to lower the temperature, culturing medicinal mushroom mycelium with cereals, and releasing the lumped parts, and drying with hot air for the first purpose, The second object of the invention is to make it possible to inoculate medicinal mushrooms (e.g., Sanghwang mushroom, cordyceps, etc.) into grains (e.g., rice, brown rice, wheat, barley, corn, etc.) as a liquid seed inoculation, and the third The purpose is to significantly reduce the number of incubation days compared to the prior art due to the use of liquid seeds, the fourth purpose is to significantly increase the yield because liquid seeds are evenly permeated into the grain, and the fifth purpose is to The purpose of the sixth purpose is to promote health by allowing consumers to easily consume functional grains containing medicinal mushrooms through their daily meals, especially noodles. The purpose of the seventh purpose is to produce high-quality grains containing medicinal mushrooms within a short period of time, so that functional grains can be supplied to consumers at a cheaper price, and the eighth purpose is the pharmacological effect of mushrooms. The synergistic effect of the unique efficacy, usability, and convenience of grains can be expected. The 10th purpose is to increase the income of growers and make a great contribution to human health, and the 11th purpose is to greatly improve the quality and reliability of functional grains, thereby satisfying the various needs (needs) of consumers who are users. Functional grains cultivated with mycelium extracts from mushrooms and a method for manufacturing the same.
이러한 목적 달성을 위하여 본 발명은 이물질을 걸러낸 곡류를 깨끗히 수세하고, 물에 침지시키는 단계; 곡류를 채반 그릇에 올려 놓고 물기를 제거하는 단계; 물기가 제거된 곡류를 병이나 비닐봉지에 입봉한 후 필터가 있는 마개를 닫아 장착하는 단계; 병이나 비닐봉지의 마개를 닫아 밀봉한 후 곡류를 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계; 멸균이 끝난 곡류를 자연 배기시켜 하온시키는 단계; 자연 배기된 곡류에 약용버섯 액체 종균 배지 조성물을 접종한 후 곡류로 약용버섯 균사체를 배양하는 단계; 및 액체 종균이 접종된 곡류의 뭉친 부분을 풀어주고, 열풍으로 건조하는 단계;가 포함됨을 특징으로 하는 버섯류의 균사체 추출물로 배양한 기능성 곡류 제조방법을 제공한다.In order to achieve this object, the present invention includes the steps of washing the grains filtered out of foreign substances with water and immersing them in water; placing the grains on a sieve bowl and draining the water; After sealing the water-removed grains into a bottle or a plastic bag, closing the cap with a filter and mounting; High-pressure sterilization (sterilization) and sterilization (sterilization) of the grain after sealing the bottle or plastic bag by closing the stopper; heating the sterilized grains by naturally evacuating them; culturing medicinal mushroom mycelium with cereals after inoculating the medicinal mushroom liquid seed culture medium composition into naturally evacuated cereals; And it provides a method for producing functional grains cultured with the mycelium extract of mushrooms, characterized in that it;
또한 본 발명은 상기 버섯류의 균사체 추출물로 배양한 기능성 곡류 제조방법을 이용하여 제조된 버섯류의 균사체 추출물로 배양한 기능성 곡류에 관한 것으로, 곡류에 약용버섯 균사체 추출물로 제조된 약용버섯 액체 종균 배지 조성물을 접종하여 약용버섯 균사체를 배양하되, 약용버섯을 곡류에 액체 종균으로 스며들게 접종할 수 있도록 하여 배양 일수를 현격히 감소시키면서 동시다발적으로 고른 배양을 유도하는 방법으로 제조된 버섯류의 균사체 추출물로 배양한 기능성 곡류를 제공한다.In addition, the present invention relates to functional grains cultured with the mycelium extract of mushrooms prepared using the method for producing functional grains cultured with the mycelium extract of mushrooms, and a medicinal mushroom liquid seed medium composition prepared with the mycelium extract of medicinal mushrooms in cereals. Functional cultured with the mycelium extract of mushrooms prepared by inoculating and culturing medicinal mushroom mycelium, but inducing a simultaneous and even culture while remarkably reducing the number of incubation days by inoculating the medicinal mushroom as a liquid starter in the grain grains are provided.
상기에서 상세히 살펴본 바와 같이 본 발명은 물에 침지시키는 단계, 물기를 제거하는 단계, 필터가 있는 마개를 닫아 장착하는 단계, 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계, 자연 배기시켜 하온시키는 단계, 곡류로 약용버섯 균사체를 배양하는 단계 및 뭉친 부분을 풀어주고, 열풍으로 건조하는 단계가 포함된 것이다.As described in detail above, the present invention includes a step of immersing in water, a step of removing water, a step of closing and mounting a cap with a filter, a step of high-pressure sterilization and sterilization treatment, a step of naturally evacuating and warming Step, the step of culturing the medicinal mushroom mycelium with cereals, and releasing the lumped part, and drying with hot air are included.
상기한 기술적 구성에 의한 본 발명은 약용버섯(예: 상황버섯, 동충하초 등)을 곡류(예: 쌀·현미·밀·보리·옥수수 등의 곡식 등)에 액체 종균으로 스며들게 접종할 수 있도록 한 것이다.The present invention according to the above technical configuration is to inoculate medicinal mushrooms (eg, Sanghwang mushroom, cordyceps, etc.) into grains (eg, rice, brown rice, wheat, barley, corn, etc.) as a liquid seed germ. .
또한 본 발명은 액체 종균을 사용함으로 인해 배양 일수를 종래에 비해 현격히 감소시킬 수 있도록 한 것이다.In addition, the present invention allows to significantly reduce the number of incubation days compared to the prior art due to the use of liquid seed bacteria.
그리고 본 발명은 액체 종균이 곡류에 고르게 스며들기 때문에 수확량을 현저하게 증가시킬 수 있도록 한 것이다.And the present invention is intended to significantly increase the yield because the liquid seed is evenly permeated into the grain.
또한 본 발명은 액체 종균이 동시다발적으로 곡류에 골고루 스며들기 때문에 고른 배양을 유도할 수 있도록 한 것이다.In addition, the present invention is intended to induce an even culture because the liquid seed bacteria permeate the grains simultaneously and evenly.
그리고 본 발명은 약용버섯이 함유된 기능성 곡류를 소비자들이 일상적인 식사 과정을 통해 손쉽게 섭취함으로 건강 증진 특히 면연력을 높일 수 있도록 한 것이다.And the present invention is to enable consumers to easily ingest functional grains containing medicinal mushrooms through a daily meal process to promote health, especially to increase immunity.
또한 본 발명은 단시간 내에 약용버섯이 함유된 고품질의 곡류를 생산하므로 소비자들에게 기능성 곡류를 보다 싼 값으로 공급할 수 있도록 한 것이다.In addition, the present invention produces high-quality grains containing medicinal mushrooms within a short period of time, so that functional grains can be supplied to consumers at a cheaper price.
특히 본 발명은 버섯이 가지는 약리 효과와 곡류가 가지는 독특한 효능 및 이용성 그리고 편의성의 상승 효과를 기대할 수 있도록 한 것이다.In particular, the present invention is to expect a synergistic effect of the pharmacological effect of mushrooms and the unique efficacy and usability and convenience of grains.
아울러 본 발명은 버섯이 가지는 약리 효과로 인해 각종 암을 예방하고 종양을 없애줄 수 있고 질병에 대하여 예방 및 억제할 수 있도록 한 것이다.In addition, the present invention is to prevent various cancers and to eliminate tumors due to the pharmacological effects of mushrooms, and to prevent and inhibit diseases.
더하여 본 발명은 재배농가의 소득을 증대시키고 인류 건강에 크게 이바지할 수 있도록 한 것이다.In addition, the present invention is to increase the income of growers and greatly contribute to human health.
본 발명은 상기한 효과로 인해 기능성 곡류의 품질과 신뢰성을 대폭 향상시키므로 사용자인 소비자들의 다양한 욕구(니즈)를 충족시켜 좋은 이미지를 심어줄 수 있도록 한 매우 유용한 발명인 것이다.The present invention is a very useful invention that can provide a good image by satisfying various needs (needs) of users, who are users, because the quality and reliability of functional grains are greatly improved due to the above-described effects.
이하에서는 이러한 효과 달성을 위한 본 발명의 바람직한 실시 예를 첨부된 도면에 따라 상세히 설명하면 다음과 같다.Hereinafter, preferred embodiments of the present invention for achieving these effects will be described in detail with reference to the accompanying drawings.
도 1 은 본 발명에 적용된 곡류를 깨끗히 수세하고, 물에 침지시킨 상태의
사진.
도 2 는 본 발명에 적용된 곡류를 채반 그릇에 올려 놓고 물기를 제거하는
상태의 사진.
도 3 은 본 발명에 적용된 물기가 제거된 곡류를 비닐봉지에 입봉한 후 필터
가 있는 마개를 닫아 장착한 상태의 사진.
도 4 는 본 발명에 적용된 곡류를 고압 멸균(sterilization) 및 살
균(sterilization) 처리한 상태의 사진.
도 5 는 본 발명에 적용된 약용버섯 액체 종균 배지 조성물을 제조하는 상태
의 사진.
도 6 은 본 발명에 적용된 곡류의 배양을 위해 약용버섯 균사체를 배양한 상
태를 보인 사진.
도 7 은 본 발명에 적용된 버섯류의 균사체 추출물로 배양한 기능성 곡류와
현미를 보인 제품 사진.
도 8 은 본 발명에 적용된 조직마쇄기의 분해 사시도.
도 9 는 본 발명에 적용된 조직마쇄기의 결합상태 사시도.1 is a state in which the grain applied to the present invention is washed cleanly and immersed in water;
picture.
2 is a view of removing water by placing the grains applied to the present invention on a tray tray;
state's photo.
3 is a filter after sealing the grains from which water has been removed applied to the present invention in a plastic bag;
A photo of the cap installed with the cap closed.
Figure 4 is high-pressure sterilization (sterilization) and fleshing the grain applied to the present invention
A photograph of the condition treated with sterilization.
5 is a state of preparing a liquid medicinal mushroom seed culture medium composition applied to the present invention
's photo.
6 is a phase of culturing medicinal mushroom mycelium for culturing cereals applied to the present invention;
A picture that showed the womb.
7 is a functional grain cultured with the mycelium extract of mushrooms applied to the present invention and
Product photo showing brown rice.
Figure 8 is an exploded perspective view of the tissue grinder applied to the present invention.
Figure 9 is a perspective view of the combined state of the tissue grinder applied to the present invention.
본 발명에 적용된 버섯류의 균사체 추출물로 배양한 기능성 곡류 및 그 제조방법은 도 1 내지 도 9 에 도시된 바와 같이 구성되는 것이다.Functional cereals cultured with the mycelium extract of mushrooms applied to the present invention and a method for producing the same are configured as shown in FIGS. 1 to 9 .
하기에서 본 발명을 설명함에 있어, 관련된 공지 기능 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명은 생략할 것이다.In the following description of the present invention, if it is determined that a detailed description of a related well-known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted.
그리고 후술되는 용어들은 본 발명에서의 기능을 고려하여 설정된 용어들로서 이는 생산자의 의도 또는 관례에 따라 달라질 수 있으므로 그 정의는 본 명세서 전반에 걸친 내용을 토대로 내려져야 할 것이다.And the terms to be described later are terms set in consideration of the function in the present invention, which may vary depending on the intention or custom of the producer, so the definition should be made based on the content throughout this specification.
또한 도면에서 나타난 각 구성의 크기 및 두께는 설명의 편의를 위해 임의로 나타내었으므로, 본 발명이 반드시 도면에 도시된 바에 한정되지 않는다.In addition, since the size and thickness of each component shown in the drawings are arbitrarily indicated for convenience of description, the present invention is not necessarily limited to the bars shown in the drawings.
먼저, 본 발명은 이물질을 걸러낸 곡류를 깨끗히 수세하고, 물에 침지시킨다.First, in the present invention, the grains from which foreign substances have been filtered are washed with water and immersed in water.
이때 본 발명에 적용된 상기 곡류는 쌀·현미·밀·보리·옥수수 등의 곡식 등을 말한다.In this case, the grains applied to the present invention refer to grains such as rice, brown rice, wheat, barley, and corn.
이후 본 발명은 상기 곡류를 채반 그릇에 올려 놓고 물기를 제거하게 된다.Thereafter, the present invention removes the water by placing the grains on a sieve bowl.
이어서 본 발명은 상기 물기가 제거된 곡류를 병이나 비닐봉지에 입봉한 후 필터가 있는 마개를 닫아 장착하게 된다.Then, in the present invention, the dried grains are sealed in a bottle or a plastic bag, and then the cap with a filter is closed and mounted.
이후 본 발명은 병이나 비닐봉지의 마개를 닫아 밀봉한 후 곡류를 고압 멸균(sterilization) 및 살균(sterilization) 처리하게 된다.After that, the present invention closes and seals a bottle or a plastic bag, and then high-pressure sterilization (sterilization) and sterilization (sterilization) treatment of the grain.
이어서 본 발명은 상기 멸균이 끝난 곡류를 자연 배기시켜 하온시키게 된다.Then, in the present invention, the sterilized grains are naturally exhausted to lower the temperature.
이후 본 발명은 자연 배기된 곡류에 약용버섯 액체 종균 배지 조성물을 접종한 후 곡류로 약용버섯 균사체를 배양하게 된다.Thereafter, in the present invention, the medicinal mushroom mycelium is cultured with the cereals after inoculating the medicinal mushroom liquid seed culture medium composition into the naturally evacuated cereals.
마지막으로 본 발명은 액체 종균이 접종된 곡류의 뭉친 부분을 풀어주고, 열풍으로 건조하여 최종 버섯류의 균사체 추출물로 배양한 기능성 곡류를 생산하게 된다.Finally, according to the present invention, the grains inoculated with liquid spawn are released and dried with hot air to produce functional grains cultured with the mycelium extract of the final mushrooms.
한편 본 발명은 상기의 구성부를 적용함에 있어 다양하게 변형될 수 있고 여러 가지 형태를 취할 수 있다.On the other hand, the present invention can be variously modified and can take various forms in applying the above-described components.
그리고 본 발명은 상기의 상세한 설명에서 언급되는 특별한 형태로 한정되는 것이 아닌 것으로 이해되어야 하며, 오히려 첨부된 청구범위에 의해 정의되는 본 발명의 정신과 범위 내에 있는 모든 변형물과 균등물 및 대체물을 포함하는 것으로 이해되어야 한다.And it is to be understood that the present invention is not limited to the particular form indicated in the above detailed description, but rather, it is intended to cover all modifications and equivalents and substitutes falling within the spirit and scope of the present invention as defined by the appended claims. should be understood as
상기와 같이 구성된 본 발명 버섯류의 균사체 추출물로 배양한 기능성 곡류 및 그 제조방법의 작용효과를 설명하면 다음과 같다.The functional effects of the functional grains cultured with the mycelium extract of the mushrooms of the present invention configured as described above and the manufacturing method thereof will be described as follows.
우선, 본 발명은 약용버섯(예: 상황버섯, 동충하초 등)을 곡류(예: 쌀·현미·밀·보리·옥수수 등의 곡식 등)에 액체 종균으로 스며들게 접종할 수 있도록 하여 배양 일수를 현격히 감소시키면서 동시다발적으로 고른 배양을 유도할 수 있도록 한 것이다.First, the present invention enables inoculation of medicinal mushrooms (e.g., Sanghwang mushroom, Cordyceps, etc.) into grains (e.g., grains such as rice, brown rice, wheat, barley, corn, etc.) as a liquid seed to inoculate, thereby significantly reducing the number of incubation days. This was done to induce simultaneous and even culture.
이를 위해 본 발명에 적용된 도 1 은 본 발명에 적용된 곡류(현미)를 깨끗히 수세하고, 물에 침지시킨 상태를 보인 사진으로, 상기 곡류(현미)를 물에 침지시키는 시간은 10~12시간이 적당한 것으로 이는 곡류(현미)의 수분율을 맞추기 위함이다.For this purpose, Fig. 1 applied to the present invention is a photograph showing a state in which the grain (brown rice) applied to the present invention is washed cleanly and immersed in water. The time for immersing the grain (brown rice) in water is suitable for 10 to 12 hours. This is to match the moisture content of grains (brown rice).
도 2 는 본 발명에 적용된 곡류(현미)를 채반 그릇에 올려 놓고 물기를 제거하는 상태를 보인 사진이다.2 is a photograph showing a state in which the grain (brown rice) applied to the present invention is placed on a tray and the water is removed.
도 3 은 본 발명에 적용된 물기가 제거된 곡류(현미)를 비닐봉지에 입봉한 후 필터가 있는 마개를 닫아 장착한 상태를 보인 사진이다.3 is a photograph showing a state in which the water-removed grain (brown rice) applied to the present invention is sealed in a plastic bag and then installed by closing the cap with a filter.
도 4 는 본 발명에 적용된 곡류(현미)를 고압 멸균(sterilization) 및 살균(sterilization) 처리한 상태를 보인 사진이다.4 is a photograph showing a state of high-pressure sterilization (sterilization) and sterilization (sterilization) of the grain (brown rice) applied to the present invention.
도 5 는 본 발명에 적용된 약용버섯 액체 종균 배지 조성물을 제조하는 상태를 보인 사진이다.5 is a photograph showing the state of preparing a liquid medicinal mushroom seed culture medium composition applied to the present invention.
도 6 은 본 발명에 적용된 곡류(현미)의 배양을 위해 약용버섯 균사체를 배양한 상태를 보인 사진이다.6 is a photograph showing the state of culturing medicinal mushroom mycelium for culturing cereals (brown rice) applied to the present invention.
도 7 은 본 발명에 적용된 버섯류의 균사체 추출물로 배양한 기능성 곡류와 현미를 보인 제품 사진이다.7 is a product photograph showing functional grains and brown rice cultured with the mycelium extract of mushrooms applied to the present invention.
상기 사진에서 오른쪽 희고 누런 곡류는 현미를 보인 것이고, 왼쪽 밤색 곡류는 현미에 버섯류 균사체가 배양된 상태를 보인 것이다.In the photo, the right white and yellow grains show brown rice, and the left chestnut grains show the cultured state of mushroom mycelium in brown rice.
도 8 은 본 발명에 적용된 조직마쇄기의 분해 사시도이고, 도 9 는 본 발명에 적용된 조직마쇄기의 결합상태 사시도를 보인 것이다.8 is an exploded perspective view of the tissue grinder applied to the present invention, and FIG. 9 is a perspective view of the combined state of the tissue grinder applied to the present invention.
본 발명은 아래 각 단계를 거쳐 기능성 곡류를 생산하게 된다.The present invention produces functional grains through each of the following steps.
먼저, 본 발명은 이물질을 걸러낸 곡류를 깨끗히 수세하고, 물에 침지시키는 단계를 거친다.First, in the present invention, the grains from which foreign substances have been filtered are thoroughly washed with water and immersed in water.
이후 본발명은 상기 곡류를 채반 그릇에 올려 놓고 물기를 제거하는 단계를 거친다.Thereafter, the present invention goes through a step of removing the water by placing the grains on a tray.
이어서 본 발명은 상기 물기가 제거된 곡류를 병이나 비닐봉지에 입봉한 후 필터가 있는 마개를 닫아 장착하는 단계를 거친다.Next, the present invention goes through a step of sealing the water-removed grains into a bottle or a plastic bag, and then closing the cap with a filter to install it.
이후 본 발명은 상기 병이나 비닐봉지의 마개를 닫아 밀봉한 후 곡류를 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계를 거친다.After the present invention closes and seals the cap of the bottle or plastic bag, high-pressure sterilization (sterilization) and sterilization (sterilization) of the grain is performed.
이어서 본 발명은 상기 멸균이 끝난 곡류를 자연 배기시켜 하온시키는 단계를 거친다.Then, the present invention goes through a step of naturally evacuating the sterilized grains to lower the temperature.
이후 본 발명은 상기 자연 배기된 곡류에 약용버섯 균사체 추출물로 제조된 약용버섯 액체 종균 배지 조성물을 접종하여 약용버섯 균사체를 배양하는 단계를 거친다.Thereafter, the present invention undergoes a step of inoculating the medicinal mushroom mycelium extract prepared from the medicinal mushroom mycelium extract into the naturally evacuated grain and culturing the medicinal mushroom mycelium.
마지막으로 본 발명은 상기 액체 종균이 접종된 곡류의 뭉친 부분을 풀어주고, 열풍으로 건조하는 단계를 거쳐 버섯류의 균사체 추출물로 배양한 기능성 곡류 제조하게 된다.Finally, according to the present invention, the functional grains cultured with the mycelium extract of mushrooms are produced through the steps of loosening the lumps of the grains inoculated with the liquid seed and drying them with hot air.
이때 상기 곡류를 물에 침지시키는 시간은 10~12시간이 적당한 것으로 이는 곡류의 수분율을 맞추기 위함으로, 곡류를 10~12시간 물에 침지하면 수분율이 25~27% 가량되어서 최적으로 물기가 제거된 상태로 현미를 채반에 거를 수 있게 된다.At this time, the time for immersing the grains in water is appropriate for 10 to 12 hours. This is to match the moisture content of the grains. When the grains are immersed in water for 10 to 12 hours, the moisture content is about 25 to 27%, which is optimally removed. In this state, brown rice can be filtered on a sieve.
만일, 상기 곡류를 10시간 미만으로 물에 침지하면 수분율이 낮아져 금방 굳어지게 되고, 접종시 균의 침투가 더디게 된다. 12시간 넘게 물에 침지하면 수분율이 높아져 곡류가 쉽게 물게 되어 배양이 안되기 때문에 상기 곡류는 10~12시간 물에 침지함이 바람직하다.If the grains are immersed in water for less than 10 hours, the moisture content is lowered and hardened quickly, and the penetration of bacteria during inoculation is slow. When immersed in water for more than 12 hours, the moisture content is increased, so that the grains are easily bitten and cultured, so that the grains are preferably immersed in water for 10 to 12 hours.
본 발명은 상기 배양단계에서 동충하초 및 상황버섯을 곡류에 배양할 때 700~1000lux 광조사 하는 것으로, 이는 광조사를 하는 경우 동충하초 곡류는 붉은 색을 띠고, 상황버섯 곡류는 누런 색을 띄도록 하고, 코르디세핀(cordycepin)의 함량이 증가되도록 하는 효과를 기대할 수 있다.The present invention is to irradiate 700-1000 lux of light when culturing Cordyceps and Sangha mushroom on grains in the culturing step. The effect of increasing the content of cordycepin can be expected.
상기 광조사 때 700lux 이하가 되면 곡류에 원하는 색상을 얻을 수 없고, 1000lux 이상이면 코르디세핀(cordycepin)의 함량이 감소되기 때문에 상기 광조사는 700~1000lux가 바람직하다.When the light irradiation is 700 lux or less, the desired color cannot be obtained in cereals, and if it is 1000 lux or more, the content of cordycepin is reduced, so the light irradiation is preferably 700 to 1000 lux.
한편, 본 발명에 적용된 상기 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계는 다음의 기술이 적용된다.On the other hand, the step of the high-pressure sterilization (sterilization) and sterilization (sterilization) treatment applied to the present invention is applied the following technique.
즉, 본 발명은 곡류의 표면과 내부에 존재하는 모든 곰팡이, 세균, 바이러스, 및 영양세포 및 포자를 사멸 또는 제거시켜 무균 상태로 만들고, 미생물을 제거하여 무균상태로 만들기 위해, 곡류를 101~105℃로 30~40분 전처리로 1차 멸균하고, 곡류를 120~125℃로 30~40분 후처리로 2차 멸균함이 바람직하다.That is, the present invention kills or removes all molds, bacteria, viruses, and vegetative cells and spores present on the surface and inside of the grain to make it sterile, and removes microorganisms to make it sterile. It is preferable to first sterilize at 30-40 minutes pretreatment at ℃, and secondary sterilize cereals at 120~125℃ for 30-40 minutes after treatment.
이때 상기 1,2차 살균은 고압살균기의 온도를 1차 105도가 될때까지 올린 후 105도에서 30분 1차 증숙시킨다.At this time, in the first and second sterilization, the temperature of the high-pressure sterilizer is raised to the first 105 degrees, and then the first steaming is performed at 105 degrees for 30 minutes.
그리고 2차 살균은 121도에서 40분 멸균시켜 현미의 살균을 완성한다. 1~2차살균의 의미는 1차는 현미의 속을 연화시키는 것이고, 2차는 현미의 속과 겉표면의 멸균을 위해서다.And the second sterilization is sterilized at 121 degrees for 40 minutes to complete the sterilization of brown rice. The meaning of the 1st and 2nd sterilization is that the first is to soften the inside of brown rice, and the second is to sterilize the inside and outside of brown rice.
상기 1차 멸균시 101℃ 이하가 되면 살균 및 멸균 효과가 상실되고, 105℃ 이상이면 곡류가 건조하고 손상되기 때문에 1차 멸균 온도는 101~105℃가 바람직하다.When the primary sterilization temperature is 101° C. or less, the sterilization and sterilization effects are lost, and when it is 105° C. or more, the grains are dried and damaged. Therefore, the primary sterilization temperature is preferably 101 to 105° C.
그리고 2차 멸균시 120℃ 이하가 되면 살균 및 멸균 효과가 상실되고, 125℃ 이상이면 곡류가 건조하고 손상되기 때문에 2차 멸균 온도는 120~125℃가 바람직하다.And when the temperature is below 120°C during secondary sterilization, the sterilization and sterilization effect is lost, and when it is above 125°C, the grains are dried and damaged, so the secondary sterilization temperature is preferably 120 to 125°C.
또한 상기 1,2차 온도가 30분 이하가 되면 곡류가 숙성이 안되고, 40분 이상이면 곡류가 건조해지기 때문에 상기 온도는 30~40분이 바람직하다.In addition, if the first and second temperatures are less than 30 minutes, the grains are not ripened, and if the temperature is more than 40 minutes, the grains are dried, so the temperature is preferably 30 to 40 minutes.
특히 본 발명에 적용된 약용버섯 균사체 추출물로 제조되는 약용버섯 액체 종균 배지 조성물의 제조는 다음과 같이 구성된다.In particular, the preparation of the liquid medicinal mushroom seed culture medium composition prepared from the medicinal mushroom mycelium extract applied to the present invention is configured as follows.
즉, 본 발명의 상기 조성물은 팹톤 파우더(peptone Powder), 맥아 추출물 파우더(malt extract Powder), 효모 추출물 파우더(yeast extract Powder), 덱스트로오스(dextrose) 및 대두(soybean Powder)를 물과 혼합하여 이루어지게 된다.That is, the composition of the present invention is a peptone powder (peptone Powder), malt extract powder (malt extract Powder), yeast extract powder (yeast extract Powder), dextrose (dextrose) and soybean (soybean Powder) by mixing with water will be done
이때 상기 약용버섯 액체 종균 배지 조성물의 혼합비율은 물 1리터당 팹톤 파우더(peptone Powder)는 0.68g이고, 맥아 추출물 파우더(malt extract Powder)는 0.56g이고, 효모 추출물 파우더(yeast extract Powder)는 0.56g이고, 덱스트로오스(dextrose)는 7.5g이고, 대두(soybean Powder)는 1g이 포함됨이 바람직하다. At this time, the mixing ratio of the medicinal mushroom liquid seed culture medium composition is 0.68 g of peptone powder per 1 liter of water, the malt extract powder is 0.56 g, and the yeast extract powder is 0.56 g. and dextrose is 7.5 g, and soybean Powder is preferably included in 1 g.
또 한편, 본 발명에 적용된 상기 약용버섯 액체 종균 배지 조성물을 조직마쇄기(10)에 넣고, 2~3분 동안 분쇄시키고, 이후 증류수에 혼합한 후 배양병(탱크)에 접종하게 된다.On the other hand, the medicinal mushroom liquid seed culture medium composition applied to the present invention is put into the
이때 상기 조직마쇄기(10)는 유리관(16)과, 유리관에 삽입되어 작동되는 몸체(11)가 구비되되, 상기 몸체(11)의 하단에는 외주면에 미세한 요철부(13)가 형성된 분쇄봉(12)이 구비되고, 몸체(11)의 상단에는 손잡이(14)와 핸들(15)이 구비되어 유리관(16)에 액체 종균 배지 조성물과 증류수를 넣고 분쇄봉(12)을 회전시키면서 조직을 분쇄시키게 된다.At this time, the
상기 분쇄봉(12)의 외주면과 유리관(16)의 내주면 사이의 공간은 0.010~0.015mm로 조직을 용이하게 분쇄시킬 수 있도록 해주게 된다.The space between the outer circumferential surface of the grinding
본 발명은 상기한 제조방법을 이용하여 제조된 버섯류의 균사체 추출물로 배양한 기능성 곡류를 제공하는 것으로, 곡물로 약용버섯 균사체를 배양하되, 약용버섯을 곡류에 액체 종균으로 스며들게 접종할 수 있도록 하여 배양 일수를 현격히 감소시키면서 동시다발적으로 고른 배양을 유도하는 방법으로 제조된 버섯류의 균사체 추출물로 배양한 기능성 곡류를 제공한다.The present invention is to provide a functional grain cultured with the mycelium extract of mushrooms prepared by using the above-described manufacturing method, wherein the medicinal mushroom mycelium is cultured with the grain, and the medicinal mushroom is inoculated to the grain as a liquid seed. Provided is a functional grain cultured with the mycelium extract of mushrooms prepared by a method of inducing a simultaneous and even culture while remarkably reducing the number of days.
(시험예 1)(Test Example 1)
상기한 본 발명에 의한 버섯류의 균사체 추출물로 배양한 기능성 곡류를 수원여자대학교 식품분석연구센터에 접수번호 A5-191114-106-01, A5-191114-106-02, A5-191114-106-03 및 A5-190509-003-01로 시험을 의뢰하여 2019년 05월 09일과 11월 14일자로 그 시험결과를 통보 받았다.Functional grains cultured with the mycelium extract of mushrooms according to the present invention as described above were submitted to the Food Analysis Research Center of Suwon Women's University with application numbers A5-191114-106-01, A5-191114-106-02, A5-191114-106-03 and The test was requested by A5-190509-003-01 and the test results were notified on May 09 and November 14, 2019.
(시험성적서 1)(Test report 1)
시험결과 총 아폴라톡신이 불검출임을 확인할 수 있다.As a result of the test, it can be confirmed that total apollatoxin is not detected.
(시험성적서 2)(Test report 2)
시험결과 납, 카드뮬, 비소 및 수은의 결과치가 합격 기준치인 미량이 검출되었음을 확인할 수 있다.As a result of the test, it can be confirmed that trace amounts of lead, card mule, arsenic and mercury, which are the pass criteria, were detected.
(시험성적서 3)(Test report 3)
시험결과 대장균구가 음성임을 확인할 수 있다.As a result of the test, it can be confirmed that E. coli cells are negative.
(시험성적서 4)(Test report 4)
시험결과 분석을 실시한 Abamectin 외 319종에서 검출된 성분을 제외한 나머지 성분은 불검출로 나타났음을 확인할 수 있다.It can be confirmed that the rest of the components except for the components detected in Abamectin and other 319 types, which were analyzed for the test results, were found to be non-detectable.
상기와 같이 본 발명은 약용버섯(예: 상황버섯, 동충하초 등)을 곡류(예: 쌀·현미·밀·보리·옥수수 등의 곡식 등)에 액체 종균으로 스며들게 접종할 수 있도록 하여 배양 일수를 현격히 감소시키면서 동시다발적으로 고른 배양을 유도할 수 있도록 한 효과를 제공하게 된다.As described above, the present invention enables the inoculation of medicinal mushrooms (e.g., Sanghwang mushroom, Cordyceps, etc.) to grains (e.g., rice, brown rice, wheat, barley, corn, etc.) as a liquid seed to inoculate, thereby significantly increasing the number of culture days. It provides the effect of inducing an even culture at the same time while decreasing.
본 발명 버섯류의 균사체 추출물로 배양한 기능성 곡류 및 그 제조방법의 기술적 사상은 실제로 동일결과를 반복 실시 가능한 것으로, 특히 이와 같은 본원발명을 실시함으로써 기술발전을 촉진하여 산업발전에 이바지할 수 있어 보호할 가치가 충분히 있다.The technical idea of functional cereals cultured with the mycelium extract of mushrooms of the present invention and their manufacturing method is that the same results can be repeatedly implemented. well worth it
<도면의 주요 부분에 대한 부호의 설명>
10: 조직마쇄기
11: 몸체
12: 분쇄봉
13: 요철부
14: 손잡이
15: 핸들
16: 유리관<Explanation of symbols for main parts of the drawing>
10: tissue grinder
11: body
12: grinding rod
13: uneven part
14: handle
15: handle
16: glass tube
Claims (5)
이물질을 걸러낸 곡류를 깨끗히 수세하고, 곡류의 수분율을 맞추기 위해 곡류를 물에 10~12시간 침지시키는 단계;
곡류를 채반 그릇에 올려 놓고 물기를 제거하는 단계;
물기가 제거된 곡류를 병이나 비닐봉지에 입봉한 후 필터가 있는 마개를 닫아 장착하는 단계;
병이나 비닐봉지의 마개를 닫아 밀봉한 후 곡류를 1차 101~105℃, 2차 120~125℃로 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계;
멸균이 끝난 곡류를 자연 배기시켜 하온시키는 단계;
자연 배기된 곡류에 약용버섯 균사체 추출물로 제조된 약용버섯 액체 종균 배지 조성물을 접종하여 약용버섯 균사체를 배양하는 단계; 및
액체 종균이 접종된 곡류의 뭉친 부분을 풀어주고, 열풍으로 건조하는 단계; 및
상기 배양단계에서 동충하초 및 상황버섯을 곡류에 배양할 때 700~1000lux 광조사 하고,
상기 약용버섯 액체 종균 배지 조성물은 팹톤 파우더(peptone Powder), 맥아 추출물 파우더(malt extract Powder), 효모 추출물 파우더(yeast extract Powder), 덱스트로오스(dextrose) 및 대두(soybean Powder)를 물과 혼합하여 이루어지되, 상기 약용버섯 액체 종균 배지 조성물의 혼합비율은 물 1리터당 팹톤 파우더(peptone Powder)는 0.68g이고, 맥아 추출물 파우더(malt extract Powder)는 0.56g이고, 효모 추출물 파우더(yeast extract Powder)는 0.56g이고, 덱스트로오스(dextrose)는 7.5g이고, 대두(soybean Powder)는 1g이 포함되되,
상기 약용버섯 액체 종균 배지 조성물을 조직마쇄기(10)에 넣고, 2~3분 동안 분쇄시키고, 이후 증류수에 혼합한 후 배양병에 접종하되, 상기 조직마쇄기(10)는 유리관(16)과, 유리관에 삽입되어 작동되는 몸체(11)가 구비되되, 상기 몸체(11)의 하단에는 외주면에 미세한 요철부(13)가 형성된 분쇄봉(12)이 구비되고, 몸체(11)의 상단에는 손잡이(14)와 핸들(15)이 구비되어 유리관(16)에 액체 종균 배지 조성물과 증류수를 넣고 분쇄봉(12)을 회전시키면서 조직을 분쇄시킴을 특징으로 하는 버섯류의 균사체 추출물로 배양한 기능성 곡류 제조방법.
It relates to a method for producing functional grains cultured with a mycelium extract of mushrooms for inducing a simultaneous and even culture while reducing the number of culture days by allowing medicinal mushrooms to be inoculated into grains with a liquid starter,
Washing the grains from which foreign substances have been filtered, and immersing the grains in water for 10 to 12 hours to adjust the moisture content of the grains;
placing the grains on a sieve bowl and draining the water;
After sealing the water-removed grains into a bottle or a plastic bag, closing the cap with a filter and mounting;
After sealing the bottle or plastic bag by closing the cap, the first step of high-pressure sterilization (sterilization) and sterilization (sterilization) treatment at 101 ~ 105 ℃, the second 120 ~ 125 ℃;
heating the sterilized grains by naturally evacuating them;
culturing medicinal mushroom mycelium by inoculating a medicinal mushroom liquid seed culture medium composition prepared with medicinal mushroom mycelium extract into naturally evacuated grains; and
releasing the lumps of grains inoculated with liquid seeds, and drying with hot air; and
In the culturing step, when culturing Cordyceps cordyceps and Sanghuang mushrooms on cereals, irradiate with 700-1000 lux light,
The medicinal mushroom liquid seed culture medium composition is a peptone powder (peptone Powder), malt extract powder (malt extract Powder), yeast extract powder (yeast extract Powder), dextrose (dextrose) and soybean (soybean Powder) mixed with water However, the mixing ratio of the medicinal mushroom liquid seed culture medium composition is 0.68 g of peptone powder per 1 liter of water, and the malt extract powder is 0.56 g, and the yeast extract powder is 0.56 g, dextrose is 7.5 g, soybean Powder is 1 g,
Put the medicinal mushroom liquid seed culture medium composition into a tissue grinder 10, grind for 2-3 minutes, and then mix with distilled water and inoculate into a culture bottle, wherein the tissue grinder 10 includes a glass tube 16 and , is provided with a body 11 that is inserted and operated in a glass tube, the lower end of the body 11 is provided with a grinding rod 12 having fine concavo-convex portions 13 formed on the outer circumferential surface, and a handle is provided at the upper end of the body 11 . (14) and a handle (15) are provided to put a liquid seed medium composition and distilled water into a glass tube (16), and to grind the tissue while rotating the grinding rod (12). method.
상기 고압 멸균(sterilization) 및 살균(sterilization) 처리하는 단계는,
곡류의 표면과 내부에 존재하는 모든 곰팡이, 세균, 바이러스, 및 영양세포 및 포자를 사멸 또는 제거시켜 무균 상태로 만들고, 미생물을 제거하여 무균상태로 만들기 위해,
곡류를 101~105℃로 30~40분 전처리로 1차 멸균하고,
곡류를 120~125℃로 30~40분 후처리로 2차 멸균함을 특징으로 하는 버섯류의 균사체 추출물로 배양한 기능성 곡류의 제조방법.
The method according to claim 1,
The high-pressure sterilization (sterilization) and sterilization (sterilization) processing step,
To make sterile by killing or removing all molds, bacteria, viruses, and vegetative cells and spores present on the surface and inside of grains, and to make them sterile by removing microorganisms;
First sterilize grains by pretreatment at 101~105℃ for 30~40 minutes,
A method for producing functional grains cultured with mycelium extract of mushrooms, characterized in that the grains are secondarily sterilized by post-treatment at 120~125℃ for 30~40 minutes.
곡류에 약용버섯 균사체 추출물로 제조된 약용버섯 액체 종균 배지 조성물을 접종하여 약용버섯 균사체를 배양하되, 약용버섯을 곡류에 액체 종균으로 스며들게 접종할 수 있도록 하여 배양 일수를 현격히 감소시키면서 동시다발적으로 고른 배양을 유도하는 방법으로 제조된 버섯류의 균사체 추출물로 배양한 기능성 곡류.It relates to functional cereals cultured with the mycelium extract of mushrooms prepared by using the manufacturing method of any one of claims 1 to 2,
The medicinal mushroom mycelium was cultured by inoculating grains with a medicinal mushroom liquid spawning medium composition prepared with medicinal mushroom mycelium extract, but the medicinal mushroom was inoculated to permeate the grain as a liquid spawn, so that the number of cultivation days was significantly reduced while simultaneously selecting Functional cereals cultured with mycelium extracts of mushrooms prepared by a method of inducing culture.
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