KR102147368B1 - Immunochromatography Sensor Cartrige - Google Patents

Abstract

The present invention is an immunochromatography sensor cartridge, comprising: a cover portion having a display portion formed therethrough to expose a measurement area of an immunochromatography sensor strip (hereinafter referred to as'strip'), and a support for receiving the strip on the upper surface Including a portion, the cover portion or the support portion is provided with a solution barrier region forming portion for forming a barrier region by pressing or removing a predetermined region of the porous membrane adjacent to the measurement region exposed by the display portion by external pressure.

Description

Immunochromatography Sensor Cartridge {Immunochromatography Sensor Cartrige}

The present invention relates to immunochromatographic technology, and to an immunochromatographic sensor for testing a sample to be analyzed.

Immunochromatographic analysis is a test technique for diagnosing a disease using an immune response in which an antigen-antibody binds. In other terms, it is also called a lateral flow assay. The most common example that can be seen around us is a pregnancy diagnostic kit that can use urine to determine whether you are pregnant, and in addition, it is used to quickly check the presence or absence of disease in samples from the human body such as hepatitis, pneumonia, and influenza.

Immunochromatographic tests, commonly referred to as rapid-kits, can provide results with low cost and fast test time. The principle is that antibodies that specifically bind to disease-causing antigens are combined with gold nanoparticles to obtain conjugation. After immobilizing on the gate pad, the other type of antibody is immobilized on the porous membrane, and then the sample to be tested is absorbed by the sample pad to proceed along the porous membrane.

If there is an antigen in the sample, the antibody bound to the gold nanoparticles of the conjugate pad binds and then passes along the porous membrane and binds to the antibody immobilized on the test line, and the color of the gold nanoparticles appears, indicating positive. Antibodies to which antigen is not bound are bound at the control line, and the color of gold nanoparticles appears.

These immunochromatographic tests require a high analysis signal/background signal ratio to measure an analyte with high sensitivity, thereby increasing its accuracy.

The present invention provides an immunochromatography sensor cartridge capable of measuring an analysis signal with high sensitivity by increasing an analysis signal/background signal ratio in order to increase the accuracy of an immunochromatographic test result.

The present invention is an immunochromatography sensor cartridge, comprising: a cover portion having a display portion formed therethrough to expose a measurement area of an immunochromatography sensor strip (hereinafter referred to as'strip'), and a support for receiving the strip on the upper surface Including a portion, the cover portion or the support portion is provided with a solution barrier region forming portion for forming a barrier region by pressing or removing a predetermined region of the porous membrane adjacent to the measurement region exposed by the display portion by external pressure.

The present invention increases the analysis signal/background signal ratio in the measurement area of the porous membrane of the immunochromatography sensor strip so that the analysis signal can be measured with high sensitivity, thereby increasing the accuracy of the immunochromatography test result.

That is, the present invention minimizes or blocks the flow of a washing solution to clean the measurement area after passing a sample, which is an analyte, to the measurement area of the porous membrane of immunochromatography, thereby removing false positives of the analysis signal. Improve.

In addition, the present invention minimizes or blocks the flow of the signal amplification solution dripping after the sample as an analyte passes through the measurement area of the porous membrane of immunochromatography, thereby increasing the sensitivity by increasing the analysis signal/background signal ratio in the measurement area.

1 is a structural diagram of an immunochromatography sensor strip.
2 is a view for explaining the flow of a solution when a solution is dropped onto a sample pad or a porous membrane.
3 is a diagram showing an aspect of a process of forming a solution blocking region for immunochromatography measurement according to the present invention.
4 is a diagram showing another aspect of a process of forming a solution blocking region for immunochromatographic measurement according to the present invention.
5 is a view showing various embodiments of a method of forming a solution blocking region on a porous membrane according to the present invention.
6 is a plan view of an immunochromatography sensor cartridge according to a first embodiment of the present invention.
7 is an AA′ cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 6.
8 is a view showing a state of use of the immunochromatography sensor cartridge shown in FIG. 6.
9 is a plan view of an immunochromatography sensor cartridge according to a second embodiment of the present invention.
10 is an AA′ cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 9.
11 is a view showing a state of use of the immunochromatography sensor cartridge shown in FIG. 9.
12 is a plan view of an immunochromatography sensor cartridge according to a third embodiment of the present invention.
13 is an AA′ cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 12.
14 is a view showing a state of use of the immunochromatography sensor cartridge shown in FIG. 12.
15 is a plan view of an immunochromatography sensor cartridge according to a fourth embodiment of the present invention.
16 is an AA′ cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 15.
17 is a view showing a state of use of the immunochromatography sensor cartridge shown in FIG. 15.
18 is a plan view of an immunochromatography sensor cartridge according to a fifth embodiment of the present invention.
19 is an AA′ cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 18.
FIG. 20 is a diagram showing a state of use of the immunochromatography sensor cartridge shown in FIG. 18;
21 is a perspective view of an immunochromatography sensor cartridge according to a sixth embodiment of the present invention.
22 is a plan view of the immunochromatography sensor cartridge shown in FIG. 21 in an unfolded state.
23 is a plan view of the immunochromatography sensor cartridge shown in FIG. 21 in a folded state.
FIG. 24 is a cross-sectional view of the immunochromatographic sensor cartridge shown in FIG.
25 is a plan view of an immunochromatography sensor cartridge according to a seventh embodiment of the present invention.
26 is an AA′ cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 25.
27 is a structural diagram of an immunochromatographic sensor strip according to another embodiment of the present invention.
28 is a diagram illustrating a state in which a solution overflows in a porous membrane.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in a variety of different forms, only the present embodiments are intended to complete the disclosure of the present invention, and the general knowledge in the technical field to which the present invention pertains. It is provided to completely inform the scope of the invention to those who have it, and the invention is only defined by the scope of the claims. The same reference numerals refer to the same components throughout the specification.

In describing embodiments of the present invention, if it is determined that a detailed description of a known function or configuration may unnecessarily obscure the subject matter of the present invention, a detailed description thereof will be omitted, and terms to be described later are in the embodiments of the present invention. These terms are defined in consideration of the function of the user and may vary according to the intention or custom of users or operators. Therefore, the definition should be made based on the contents throughout this specification.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the embodiments of the present invention exemplified below may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below. Embodiments of the present invention are provided to more completely describe the present invention to those of ordinary skill in the art.

1 is a structural diagram of an immunochromatography sensor strip.

Referring to FIG. 1, a measurement area in a porous membrane 2 having one end 2a and the other end 2b of an immunochromatography sensor strip (hereinafter referred to as'strip') 1 (3) is provided, one end (2a) of the porous membrane (2) is stacked with a conjugate pad (5), and the other end (2b) of the porous membrane (2) is an absorption pad (absorption pad) ( 7) are stacked. In the conjugate pad 5, a sample pad 6 is stacked at an end opposite to the portion where the porous film 2 is stacked.

Here, the meaning of lamination means that when a liquid is dropped on one layer, it moves to another layer that is stacked. This laminated structure is formed by the adhesive layer 4a present in the polymer film.

The 3D porous membrane 2 has horizontal, vertical, and height (thickness), and micro porous is connected to each other in a horizontal, vertical, and high three-dimensional direction. Capillary action occurs due to the interconnected micropores, and when the solution is dropped, it moves in three dimensions. For example, the pores of the 3D porous membrane 2 have a symmetric structure having a shape similar in size on both sides and an asymmetric structure in which the size of the pores on one side and the pore size on the other side are different. It is common. The average pore size is 0.2um~5um.

In addition, the porous membrane 2 is hydrophilic or hydrophobic. In addition, the porous membrane 2 generally has a positive charge, an anion, or is neutral.

Porous membrane (2) selectively binds to proteins or peptides, aptamers, DNA, RNA, and ligand analytes such as antibodies, enzymes, and antigens. The material to be measured may be physically adsorbed or chemically bonded to form the measurement region 3. In particular, in order to induce chemical bonding, a functional group such as aldehyde may be formed on the porous membrane 2.

In general, one surface of the porous membrane 2 is generally bonded to a non-porous polymer film 4, and there are cases where the polymer film 4 is not present. The material of the porous membrane 2 generally used in the immunochromatography sensor strip 1 is a polymer of Nitrocellulose (NC), Nylon, Polyethersulfone, Polypropylene, Polysulfone, and Polyvinylidene fluoride (PVDF).

In the measurement region 3 formed in the porous membrane 2, a number of analytes can be simultaneously measured in the same sample as when one analyte is measured in the same sample. In order to measure each analyte, the measurement area 3 may have a plurality of lines in the length of the width of the porous membrane 2, may be circular, or may be formed of a plurality of spots. In general, the antibody or antigen binding to the analyte can form the measurement region (3).

The conjugate is a form in which an analysis signal is directly generated by selectively binding to the measurement area 3 of the porous membrane 2, or a material capable of generating an analysis signal is combined with a material that selectively binds to an analyte. .

Materials that directly generate the analysis signal are particles such as gold nanoparticles, silver nanoparticles, colored latex particles, magnetic particles, and quantum dot particles. , A fluorescence material, and the like, and may include a material generally used in the same industry. Antibodies, antigens, peptides, aptamers, DNA, RNA, etc. are bound to substances that directly generate an analysis signal to form a conjugate.

-Galactosidase가 적합하며, AP 효소 기질은 PNPP(p-Nitrophenyl Phosphate), BCIP/NBT(5-bromo-4-chloro-3-indolyl-phosphate/nitroblue tetrazolium) 등이 적합하며, HRP 효소 기질은 ABTS(2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]), OPD(o-phenylenediamine dihydrochloride), TMB(3,3',5,5'-tetramethylbenzidine), DAB(3,3',4,4' diaminobenzidine), 4-CN(4-chloro-1-naphthol) 등이 적합하다. 특히 발색 기질이 효소와 반응 후 다공성 막(2)에서 침전이(precipitation) 일어나는 것이 바람직하다. 그럼으로 특히 BCIP/NBT와 TMB가 적합하다. ALP와 반응하여 화학발광(chemiluminescent)하는 효소 기질은 methylumbelliferyl phosphate (MUP), 1,2-dioxetane 유도체 등을 사용가능하다. 항체, 항원, 펩타이드, 압타머, DNA, RNA 등이 효소에 결합되어 컨쥬게이트를 형성한다In addition, a substance capable of generating an analysis signal by forming a conjugate may be an enzyme, and although the enzyme itself does not generate an analysis signal, it reacts with the enzyme substrate to produce a color signal, a fluorescence signal, and a chemiluminescent signal. ) Signal can be generated. In particular, the enzymes are alkaline phosphatase (AP), horseradish peroxidase (HRP),

-Galactosidase is suitable, and the AP enzyme substrate is PNPP (p-Nitrophenyl Phosphate), BCIP/NBT (5-bromo-4-chloro-3-indolyl-phosphate/nitroblue tetrazolium), etc., and the HRP enzyme substrate is ABTS ( 2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]), OPD(o-phenylenediamine dihydrochloride), TMB(3,3',5,5'-tetramethylbenzidine), DAB(3,3',4, 4'diaminobenzidine), 4-CN (4-chloro-1-naphthol), etc. are suitable. Particularly, it is preferable that the colored substrate reacts with the enzyme and then precipitation occurs in the porous membrane 2. Therefore, BCIP/NBT and TMB are particularly suitable. As an enzyme substrate that reacts with ALP and performs chemiluminescent, methylumbelliferyl phosphate (MUP), 1,2-dioxetane derivatives, etc. can be used. Antibodies, antigens, peptides, aptamers, DNA, RNA, etc. are bound to enzymes to form conjugates.

The conjugate is provided on the conjugate pad 5 in a dried state. The conjugate pad 5 is generally stacked on one end 2a of the porous membrane 2 in the immunochromatography method, and has a structure stacked with the sample pad 6 as well. When a solution or an analysis sample is dropped onto the sample pad 6, the solution moves from the sample pad 6 through the conjugate pad 5 to the porous membrane 2, and at this time, the conjugate also moves. In order to simultaneously measure various analytes present in the same sample, a plurality of conjugates may be provided on a plurality of conjugate pads and stacked, and a plurality of conjugates may be provided on a single conjugate pad. In addition, the conjugate is not provided on the conjugate pad, but is stored in a container such as a tube, and then a measurement sample is added to this container, and the conjugate mixed with the sample is placed on the sample pad (6) or the porous membrane (2). Can be directly instilled on.

The sample pad 6 is stacked on the conjugate pad 5 and functions to supply an analysis sample to the conjugate pad 5 at a constant flow rate. In addition, when the analysis sample contains blood cells such as red blood cells (RBC) and white blood cells (WBC), it plays a role of filtering these blood cells. Also, when the analysis sample is feces or secreted substances in the nose, it affects the measurement. It performs the function of filtering substances.

The absorbent pad 7 is generally stacked on the other end 2b of the porous membrane 2 and serves to absorb the liquid introduced into the porous membrane 2.

The absorbent pad 7, the porous membrane 2, the conjugate pad 5, and the sample pad 6 described in this patent are known techniques such as immunochromatography or lateral flow immunoassay. LFA) can be used. In addition, conjugate fabrication and measurement region 3 fabrication can also be fabricated using known techniques.

The solution applied to the sample pad 6 or the porous membrane 2 may be an analysis sample, a washing solution, or a signal enhancing solution. For example, a solution dripping onto the sample pad 6 is an analysis sample, and a solution dripping onto the porous membrane 2 is a washing solution or a signal amplification solution.

The cleaning solution performs a function of washing the conjugate present in the porous membrane 2 except for the conjugate selectively bound to the analyte in the measurement region 3 of the porous membrane 2. The washing solution may be composed of a buffer solution, and a surfactant and a preservative may be added. In addition, it may include a material that generates an analysis signal by mixing the signal amplification solution and the measurement region 3. For example, it may include an oxidizing agent and a reducing agent, and as an example, it may include a reducing agent that reduces gold ions and silver ions. It may also contain hydrogen peroxide, which is used as a substrate for enzymes.

The signal amplification solution is a solution containing a substance that generates an analysis signal by reacting or combining with the conjugate of the measurement region 3. The material generating the analysis signal is metal ions such as gold ions and silver ions, or includes an enzyme substrate that reacts with an enzyme to generate an analysis signal.

In addition, a mixed solution that combines the function of the cleaning solution and the function of the signal amplification solution may be used.

2 is a view for explaining the flow of a solution when a solution is dropped onto a sample pad or a porous membrane.

2A shows the flow of the solution when the sample is dropped onto the sample pad 6, and the sample is an analysis sample containing an analyte. In the process of moving the dropped sample to the absorption pad (7), the conjugate also moves and passes through the measurement area (3), and the second antibody and the second antibody and the second antibody fixed to the measurement area (3)-analyte-first antibody -A gold nanoparticle complex is formed (sandwich immunoassay using gold nanoparticles). The sample deposited on the sample pad 6 can be moved to the absorbent pad 7 through the porous membrane 2 for at least 10 minutes, and the gold nanoparticles dried and introduced into the conjugate pad 5 during the movement are also Move the porous membrane 2 together. Therefore, gold nanoparticles also exist in the porous membrane 2 wet with the sample.

2B shows the flow of the solution when the first solution is instilled on the porous membrane 2, the first solution is a washing solution, and the second antibody in the measurement area 3 -analysis The gold nanoparticles present in the porous membrane 2 excluding the substance-first antibody-gold nanoparticle complex are washed. However, a part of the first solution dropped on the porous membrane 2 moves to the conjugate pad 5, and the gold particles present in the conjugate pad 5 are continuously moved to the absorption pad 7 to prevent cleaning. There may be no effect. In addition, when the analysis sample dropped on the sample pad 6 is a whole blood sample containing red blood cells (RBC), the red blood cells are hemolysis by the first solution and may flow into the porous membrane 2. have.

Therefore, even if the first solution is dropped onto the porous membrane 2, the analysis signal/background signal ratio does not increase, and thus the analyte cannot be measured with high sensitivity.

In addition, the first solution may include, for example, a reducing agent that reduces silver ions. The reducing agent reduces silver ions contained in the second solution on the surface of gold particles, so that silver grows on the surface of gold particles. Silver grown on the surface of gold particles plays a role of amplifying the analysis signal in the measurement area 3 by generating large particles. This technique is already known.

Fig. 2(c) shows the flow of the solution when the second solution is dropped onto the porous membrane 2, and when a certain time elapses after dropping the first solution, the second solution is dropped onto the porous membrane 2 . The gold particles present in the measurement region 3 by the reducing agent of the first solution and the silver ions of the second solution increase in size by reduction of the silver ions to amplify the analysis signal.

-Galactosidase를 사용할 수 있다. In addition, a second antibody selectively binding to an analyte is immobilized in the measurement region (3) of the porous membrane (2), and an enzyme is immobilized on the first antibody selectively binding to an analyte instead of gold nanoparticles. The conjugate is prepared and dried and introduced into the conjugate pad (5). Enzymes used for conjugate production are AP (alkaline phosphatase), HRP (horseradish peroxidase),

-Galactosidase can be used.

When an enzyme is used as a conjugate, the second solution contains an enzyme substrate, and the enzyme substrate reacts with the enzyme to develop color or emit light in the measurement region 3.

By the first solution, the enzyme except for the second antibody-analyte-first antibody-enzyme complex in the measurement region 3 is washed in the porous membrane 2. It is important that the enzyme substrate reacts with the enzyme in the measurement region 3 to develop color to generate an analysis signal and is precipitated in the second solution. This is because after dropping the second solution, the second solution continuously moves to the absorbent pad 7, and if the colored substrate is moved together during the transfer process, a problem occurs in analyzing the measurement area 3. BCIP/NBT or TMB is suitable as an enzyme substrate in which precipitation occurs after color development.

However, most of the enzyme substrates do not form precipitation in the second solution after reaction with the enzyme, so when the second solution moves in the porous membrane 2 as shown in FIG. 2(c), the enzyme reacts with the enzyme. It is difficult to measure the analysis signal in the measurement area 3 because the substrate is also moved.

That is, even if a washing solution or a signal enhancing solution is used to measure an analyte with high sensitivity, conjugates such as gold particles and enzymes from the conjugate pad (5) are transferred to the porous membrane (2). Either continuously inflow, red blood cells, etc. from the sample pad 6 flow into the porous membrane 2, or the substrate reacted with the enzyme in the measurement area 3 moves to the absorption pad 7 The analysis signal is blurred or the background signal is amplified to decrease the analysis sensitivity.

Therefore, in order to solve the above-described problem, the present invention blocks or minimizes the flow of the first solution or the second solution by the solution blocking region as shown in FIGS. 3 and 4 to increase the analysis signal. There is a purpose.

3 is a diagram showing an aspect of a process of forming a solution blocking region for immunochromatography measurement according to the present invention, and FIG. 4 is a view showing a process of forming a solution blocking region for immunochromatography measurement according to the present invention. It is a diagram showing another aspect.

As shown in (a) of FIG. 3, after the sample is dropped onto the sample pad 6, the measurement region 3 is wetted by the sample including the conjugate according to the present invention, that is, the sample is a porous membrane. If it is determined that the measurement area 3 of (2) has passed, a solution blocking area is formed as shown in (b) of FIG. 3.

Referring to FIG. 3B, the solution blocking region may be formed between the conjugate pad 5 and the one end 2a of the stacked porous membrane 2 and the measurement region 3. When the solution blocking region is formed, the sample flowing from the conjugate pad 5 is completely blocked from moving to the measurement region 3, or at least the speed at which the sample moves to the measurement region 3 may be reduced.

In addition, in dripping the first solution and the second solution onto the porous membrane 2, preferably, as shown in FIG. 3(c), between the other end 2b of the porous membrane 2 and the solution blocking region The first solution is dropped into the area, and more preferably, the first solution is dropped between the measurement area 3 and the solution blocking area. In addition, as shown in (d) of FIG. 3, a second solution is instilled between the absorption pad 7 and the other end (2b) of the stacked porous membrane 2 and the solution blocking region, and more preferably A second solution is dropped in the measurement area 3 and the solution blocking area. Even more preferably, the second solution is dropped onto the measurement area 3. Accordingly, conjugates such as gold particles and enzymes from the conjugate pad 5 do not continuously flow into the porous membrane 2, or red blood cells, etc. from the sample pad 6 do not flow into the porous membrane 2, The cleaning effect can be large.

Referring to (d) of FIG. 4, after a certain period of time after dropping the first solution, the solution blocking area between the measurement area 3 of the porous membrane 2 and the other end 2b stacked with the absorption pad 7 Can be further formed. As a result, two solution blocking regions are formed between the conjugate pad 5 and the absorbent pad 7 based on the measurement region 3 of the porous membrane 2. Therefore, as shown in Fig. 4E, the second solution to be dropped is preferably dropped between the two solution blocking areas. The dripping second solution is blocked or restricted from moving toward the absorption pad 7 or the conjugate pad 5 by the two solution blocking regions, so that the amplification of the analysis signal may be increased. Thereby, the substrate reacted with the enzyme in the measurement region 3 is prevented from moving to the absorption pad 7 to amplify the analysis signal of the measurement region 3 to improve the analysis sensitivity.

The present invention provides an immunochromatography sensor cartridge including a solution blocking region forming portion to increase the sensitivity of measurement.

5 is a view showing various embodiments of a method of forming a solution blocking region on a porous membrane according to the present invention.

FIG. 5A is a cross-sectional view of the porous membrane 2 before formation of the solution blocking region, and pores are distributed throughout the porous membrane 2.

5B shows a solution blocking region formed by physically pressing the porous membrane 2 wet with the solution. The porous structure of the porous membrane 2 is easily pressed by external pressure, and the three-dimensionally formed micropores are broken. If the microporous structure is destroyed, the movement of the solution due to the capillary phenomenon may be blocked. In order to completely block the flow of the solution or block the flow of the solution as much as possible, the pressure to press the porous membrane 2 can be adjusted, the area to be pressed can be enlarged, or a plurality of solution blocking areas can be formed on the porous membrane 2 .

5(c) shows a solution blocking area formed by scraping the porous membrane 2 by physically contacting the porous membrane 2 wet with a solution, and a porous membrane laminated on the non-porous polymer film 4 ( Remove by scraping 2). In this case, since the porous membrane 2 is removed from the polymer film, the flow of the solution may be completely blocked.

In addition, a solution blocking region may be formed by performing the pressing process of (b) of FIG. 5 and the process of scraping the porous membrane 2 of (c) in parallel.

Accordingly, the solution blocking region forming unit of the present invention is a means for the inspector to press or remove a predetermined region of the porous membrane adjacent to the measurement region to form the blocking region.

Various embodiments of the solution blocking region forming unit formed on the immunochromatography sensor cartridge are possible, and will be described in detail with reference to FIGS. 6 to 26. Hereinafter, for better understanding of the present invention, the length direction of the strip is defined as the X-axis direction, the width direction of the strip is the Y-axis direction, and the direction perpendicular to the plane formed by the X-axis and the Y-axis is determined as the Z-axis direction. To

6 is a plan view of the immunochromatography sensor cartridge according to the first embodiment of the present invention, FIG. 7 is an AA′ cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 6, and FIG. 8 is It is a diagram showing the state of use of the graphics sensor cartridge.

6 to 8, the immunochromatography sensor cartridge according to the first embodiment of the present invention provides a predetermined internal space by a housing 10 formed by fastening a cover portion 11a and a support portion 11b. It may be provided, and the strip 1 may be accommodated in the inner space of the housing 10.

The cover part 11a may include a sample injection part 12, a display part 13, and a solution blocking area forming part 100. Here, an embodiment in which the solution blocking region forming part 100 is formed on the cover part 11a will be described, but the present invention is not limited thereto. That is, the solution blocking region forming part 100 may be formed on the support part 11b.

Here, the sample injection hole 12 may be recessed from the upper surface of the cover part 11a and formed through the inner space. Therefore, when the sample is injected into the sample inlet 12, the sample can flow into the inner space, and the sample permeates the sample pad 6 disposed below the sample inlet 12 and is a conjugate pad by chromatography. It moves to the measurement area 3 of the porous membrane 2 via (5).

The display portion 13 may be a type of through hole that exposes the measurement area 3 of the porous membrane 2 to the outside. Accordingly, the display portion 13 may be formed larger than the size of the measurement area 3 of the porous membrane 2, but according to the present invention, the solution blocking area can be formed in both directions of the measurement area 3 It must be long enough.

The solution blocking area forming unit 100 is a means for forming a blocking area by pressing or removing a predetermined area of the porous film adjacent to the measurement area 3 exposed by the display unit 13. Although one solution blocking region forming part 100 is shown in FIG. 6, the present invention is not limited thereto. That is, at least two of the solution blocking region forming units 100 are formed on both sides of the measurement region 3 so that at least two solution blocking regions are formed in predetermined regions of the porous membrane adjacent in both directions of the measurement region 3. I can.

According to the first embodiment of the present invention, the solution blocking region forming part 100 includes a guiding part 110 and a pressing part 120.

The guiding part 110 has one end 110a connected to one side of the through hole forming the display part 13 and is formed to extend from the end 110a in the X-axis direction.

The pressing portion 120 formed by protruding upward/downward from the other end 110b of the guiding portion 110 is formed. The pressing part 120 protrudes upward from the other end 110b of the guiding part 110 to receive external pressure, and the pressing part 120a protrudes downward from the other end 110b of the guiding part 110. As an external pressure is applied to), a contact portion 120b that contacts the upper surface of the support portion 11b or the porous membrane 2 may be formed. Here, the external pressure may be in the same form as the tester's acupressure, and this external pressure may be defined as a space vector that can be formed in a space formed by the X-axis, Y-axis, and Z-axis.

In addition, as shown in FIG. 8, the guiding portion 110 is formed of a flexible member that can bend, so that the one end 110a of the guiding portion 110 is centered as an external pressure is applied to the pressing portion 120a. Can be bent.

Therefore, in a state in which pressure is applied to the pressing portion 120a of the lower external pressure based on the Z-axis and the contact portion 120b contacts the upper surface of the support portion 11b or the porous membrane 2, one end of the guiding portion 110 ( When a continuous external pressure is applied to the pressing portion 120a in a direction perpendicular to the circumference centered on 110a), the solution blocking region forming portion 100 is bent in the form of 100A, 100B and 100C. Then, pressure is applied to the region of the porous film 2 that the contact portion 120b passes (scratch), and the porous film 2 of the region is pressed or removed, thereby forming a solution blocking region. In this case, the external pressure must have a force sufficient to form a blocking region by pressing the porous membrane 2.

9 is a plan view of an immunochromatography sensor cartridge according to a second embodiment of the present invention, FIG. 10 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 9, and FIG. 11 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. It is a diagram showing the state of use of the graphics sensor cartridge. Here, the same parts as those described above in FIGS. 6 to 8 will be omitted, and only different contents related to the solution blocking region forming unit will be described.

9 to 11, in the cover part 11a of the immunochromatography sensor cartridge according to the second embodiment of the present invention, a predetermined area on one side of the through-hole forming the display part 13 is depressed in the Y-axis direction. A passage 14 may be formed. Two or more passages 14 may be formed on either side of the measurement region 3, which corresponds to each of the two solution blocking region forming portions 200-1 and 200-2. Here, when the solution blocking region forming portions 200-1 and 200-2 are formed in the receiving portion 11b, the passage 14 may be formed in the receiving portion 11b.

According to the second embodiment of the present invention, the solution blocking region forming parts 200-1 and 200-2 include a guiding part 210 and a pressing part 220. The guiding part 210 is formed in a bar shape horizontally extending from one end 210a to the other end 210b, is accommodated in the passage 14, and may be moved in the Y-axis direction.

A pressing portion 220 formed by protruding upward/downward from the other end 210b of the guiding portion 210 is formed. The pressing part 220 protrudes upward from the other end 210b of the guiding part 210 to receive external pressure, and the pressing part 220a protrudes downward from the other end 210b of the guiding part 210. As external pressure is applied to), a contact portion 220b may be formed in contact with the upper surface of the supporting portion 11b or the porous membrane 2. Here, the external pressure may be in the same form as the tester's acupressure, and this external pressure may be defined as a space vector that can be formed in a space formed by the Y-axis and the Z-axis.

Therefore, as shown in FIG. 11, in a state where the pressure is applied by applying the pressure part 220a of the downward external pressure based on the Z-axis to the upper surface of the support part 11b or the porous membrane 2, Y When a continuous external pressure in the axial direction is applied to the pressing portion 220a, pressure is applied to the area of the porous membrane 2 that the contact portion 220b passes (scratches), and the porous membrane 2 in the area is pressed or removed. A solution blocking region may be formed.

12 is a plan view of an immunochromatography sensor cartridge according to a third embodiment of the present invention, FIG. 13 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 12, and FIG. 14 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. It is a diagram showing the state of use of the graphics sensor cartridge. Here, the same parts as those described above with reference to FIGS. 6 to 8 are omitted, and only different contents related to the solution blocking region forming unit will be described.

12 to 14, in the cover part 11a, one side of the through-hole forming the display part 13 and a predetermined area of the other side facing the side are recessed to form the passages 14a and 14b in the Y-axis direction. Can be formed. Two or more passages 14a and 14b may be formed on both sides of the measurement region 3, which corresponds to each of the two solution blocking region forming portions 300-1 and 300-2. Here, when the solution blocking region forming portions 300-1 and 300-2 are formed in the receiving portion 11b, the passage 14 may be formed in the receiving portion 11b.

The solution blocking region forming parts 300-1 and 300-2 may be formed of a guiding part 310 and a pressing part 320.

The guiding part 310 is formed in a form of a film extending horizontally from one end 310a to the other end 310b, so that one end 310a and the other end 310b are accommodated so as to correspond to the passages 14a and 14b, respectively, so that the Y-axis Can be moved in any direction. Here, the length of the guiding part 310 should be shorter than a value obtained by subtracting the width of the porous membrane from the length of the passages 14a and 14b.

A pressing portion 320 formed by protruding upward/downward from a predetermined point 310c between the one end 310a and the other end 310b of the guiding portion 310 is formed. The pressing unit 320 protrudes upward from a predetermined point 310c of the guiding unit 310 to receive external pressure, and the pressing unit 320 is protruded downward from a predetermined point 310c of the guiding unit 310. As external pressure is applied to 320a, the contact portion 320b may be formed in contact with the upper surface of the support portion 11b or the porous membrane 2. Here, the length from the one end 310a of the guiding part 310 to the predetermined point 310c should be shorter than the length of the passage 14a. In addition, the external pressure may be in the same form as the tester's acupressure, and this external pressure may be defined as a space vector that can be formed in a space formed by the Y-axis and the Z-axis.

Accordingly, as shown in FIG. 14, in a state where the pressure is applied by applying the pressure part 320a of the downward external pressure based on the Z-axis to the upper surface of the support part 11b or the porous membrane 2, the Y When a continuous external pressure in the axial direction is applied to the pressing portion 320a, pressure is applied to the area of the porous membrane 2 that the contact portion 320b passes (scratches), and the porous membrane 2 in the area is pressed or removed. A solution blocking region may be formed.

FIG. 15 is a plan view of an immunochromatography sensor cartridge according to a fourth embodiment of the present invention, FIG. 16 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 15, and FIG. 17 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. It is a diagram showing the state of use of the graphics sensor cartridge. Here, the same parts as those described above with reference to FIGS. 6 to 8 are omitted, and only different contents related to the solution blocking region forming unit will be described.

15 to 17, according to the fourth embodiment of the present invention, the solution blocking region forming portions 400-1 and 400-2 are formed in the Y-axis direction from one side of the through hole forming the display unit 13. It is extended, a support part 410 connected to the other side facing one side is formed, and the pressing part 420 is fastened by the support part 410 and a fastening member (not shown), and the fastening member (not shown) is The pressing part 420 may be rotated around the point 420c fastened to the support part 410.

Such a fastening member and fastening method may be in various forms. For example, a through hole (not shown) is formed at a position overlapping with the porous membrane in the support part 410, and the fastening member is formed extending along the diameter of the through hole. It may be to connect one side and the other side of the through hole. Then, the pressing portion 420 is fastened in such a way that a through hole is formed in the fastening point 420c, and a fastening member is fitted into the through hole of the pressing portion 420, so that a part of the pressing portion 420 is a support part 410 ), the other part may be positioned under the support part 410, that is, the pressing part 420 may be inserted into the through hole of the support part 410.

The pressing part 420 protrudes upwards around the fastening point 420c to receive external pressure, and the pressing part 420a protrudes downward to apply the external pressure to the pressing part 420a. A contact portion 420b in contact with the upper surface or the porous membrane 2 may be formed. Here, the pressing part 420a is shown to protrude upward, but the present invention is not limited thereto. Here, the contact portion 420b may be formed in a shape of a diagonal end and a sharp edge or a rake shape, which is to facilitate scraping of the porous membrane 2. In addition, the external pressure may be in the same form as the tester's acupressure, and this external pressure may be defined as a space vector that can be formed in a space formed by the X-axis, Y-axis, and Z-axis.

Therefore, as shown in FIG. 17, when continuous external pressure is applied to the pressing portion 420a in the X-axis direction, the pressing portion 420a is centered on the fastening point 420c in the order shown in 400A, 400B and 400C. Is rotated. Then, pressure is applied to the region of the porous film 2 that the contact portion 420b passes (scratch), and the porous film 2 of the region is pressed or removed, thereby forming a solution blocking region.

18 is a plan view of an immunochromatography sensor cartridge according to a fifth embodiment of the present invention, FIG. 19 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 18, and FIG. 20 is a cross-sectional view of the immunochromatography sensor cartridge shown in FIG. It is a diagram showing the state of use of the graphics sensor cartridge. Here, the same parts as those described above with reference to FIGS. 6 to 8 are omitted, and only different contents related to the solution blocking region forming unit will be described.

18 to 20, according to the fifth embodiment of the present invention, the solution blocking region forming portions 500-1 and 500-2 are formed from one side of the through hole forming the display unit 13 in the Y-axis direction. A support part 530 is formed extending and connected to the other side facing one side, and a through hole (not shown) is formed at a position overlapping with the porous membrane in the support part 530.

A rod-shaped guiding portion 510 extending from one end to the other end is fixed to one side of the through hole, and one end and the other end are fixed at different positions with respect to the Z axis. That is, it is fixed to be inclined at an angle.

The pressing part 520 is fastened by the guiding part 510 and a fastening member (not shown), and the fastening member (not shown) is such that the pressing part 520 is tilted at an angle along the guiding part 510 and moved. I can. The pressing part 520 protrudes upwards around the fastening point 520c to receive external pressure, and the pressing part 520a protrudes downward to apply the external pressure to the pressing part 520a. A contact portion 520b in contact with the upper surface or the porous membrane 2 may be formed. Here, the pressing part 520a is shown to protrude upward, but the present invention is not limited thereto. Here, the contact portion 520b may be formed in the shape of a diagonal end and a sharp edge or a rake shape, which is to facilitate scraping of the porous membrane 2. In addition, the external pressure may be in the same form as the tester's acupressure, and this external pressure may be defined as a space vector that can be formed in a space formed by the X-axis, Y-axis, and Z-axis.

According to the fifth embodiment of the present invention, the pressing part 520a protrudes upward of the support part 530, and the contact part 520b is located under the support part 530, that is, the pressing part 520 is a support part ( It may be in a form that can be moved along the through hole of 530).

Therefore, as shown in Fig. 20, the pressure is applied to the pressing portion 520a of a downward external pressure based on the Z-axis, so that the contact portion 520b touches the upper surface of the support portion 11b or the porous membrane 2, and the pressure is applied. When a continuous external pressure in the axial direction is applied to the pressing portion 520a, pressure is applied to the area of the porous membrane 2 that the contact portion 520b passes (scratches), and the porous membrane 2 in the area is pressed or removed. A solution blocking region may be formed.

FIG. 21 is a perspective view of the immunochromatography sensor cartridge according to the sixth embodiment of the present invention, FIG. 22 is a plan view of the immunochromatography sensor cartridge shown in FIG. 21 in an unfolded state, and FIG. 23 is It is a plan view of a folded state of the chromatography sensor cartridge, and FIG. 24 is a cross-sectional view showing an AA' cross-sectional view of the immunochromatography sensor cartridge shown in FIG. 23 and a state of use.

21 to 24, the housing 10' of the immunochromatography sensor cartridge according to the sixth embodiment of the present invention is formed to be folded in half and used.

That is, in the housing 10', the lower surface of the cover part 11a' and the upper surface of the support part 11b' are connected to form a bending line 15 at the center, and the bending line 15 is folded in half. When closed, the lower surface of the cover portion 11a' is formed to face the upper surface of the support portion 11b'.

The cover portion 11a' is provided with a display portion 13' formed therethrough to expose the measurement area 3 of the porous membrane 2 to the outside. Accordingly, the display portion 13 ′ may be formed to be somewhat larger than the size of the measurement area 3 and, as a result, may be formed to have a size dependent on the size of the measurement area of the measurement area 3.

In this case, since the housing 10' can be folded and unfolded, a separate sample injection unit is not required, and the sample can be dropped onto the sample pad 6 in the open state.

A pair of slits 16 parallel in the X-axis direction are formed in a predetermined area adjacent to the display portion 13' in the cover portion 11a'. 22 illustrates a pair of slits 16, but the present invention is not limited thereto. That is, two pairs of slits 16 may be formed on both sides of the measurement area 3.

The solution blocking region forming part 600 passes through a pair of slits 16 formed in the cover part 11a' in a row shape having irregularities having a predetermined length. Accordingly, as shown in FIG. 23, when the housing 10 ′ of the immunochromatography sensor cartridge is folded, the cover portion 11a ′ overlaps the solution blocking region forming part 600. At this time, a portion overlapping above the solution blocking area forming part 600 in the cover part 11a' may be a pressing part 610a, and overlapping under the cover part 11a' in the solution blocking area forming part 600 The losing part may be the contact part 620b.

Therefore, as shown in FIG. 24, in a state in which pressure is applied to the pressing portion 620a of a downward external pressure based on the Z-axis so that the contact portion 620b contacts the upper surface or the porous membrane 2 of the support portion 11b, When a continuous external pressure in the Z-axis direction is applied to the solution blocking area forming part 600, that is, when the solution blocking area forming part 600 is pulled, pressure is applied to the area of the porous membrane 2 through which the contact part 620b passes. , A solution blocking region may be formed in the corresponding region.

According to another embodiment, a pair of slits (not shown) parallel to the X-axis direction may be formed in a predetermined region adjacent to the measurement region 3 of the porous membrane 2 in the support portion 11b'. Two pairs of slits may be formed on either side of the measurement area 3. The solution blocking region forming portion (not shown) passes through a pair of slits 16 formed in the support portion 11b' in a row having irregularities having a predetermined length. Accordingly, the solution blocking region forming portion is overlapped on the support portion 11b', so that one surface of the solution blocking region forming portion in contact with the support portion 11b' may be the contact portion.

25 is a plan view of an immunochromatography sensor cartridge according to a seventh embodiment of the present invention, and FIG. 26 is a view showing a cross-sectional view AA′ of the immunochromatography sensor cartridge shown in FIG. 25.

Referring to FIG. 25, a solution dripping part 20 may be additionally formed on the cover part 11a. The solution dropping part 20 is generated when a user directly drops a washing solution or an amplifying solution after forming a solution blocking area on the porous membrane using the solution blocking area forming part 30. It is an additional element to address the risk. Here, the solution blocking region forming unit 30 may include all of the solution blocking region forming units according to the various embodiments described above.

That is, when the user directly drops the solution onto the porous membrane, it is not easy to drop a quantity. That is, when an excessive amount of solution is dropped onto the porous membrane, over-flow occurs in the porous membrane, and when over-flow occurs, the porous membrane is not washed or absorbed by an excess solution. Problems such as inability to absorb a solution may occur due to exceeding the moisture absorption capacity of the pad 6.

Therefore, the solution dropping part 20 prevents excessive dropping of the solution, so that the amount of the solution is dropped onto the porous membrane 2. In addition, the solution dropping unit 20 adjusts the flow rate of the solution supplied to the porous membrane 2 to be supplied to the porous membrane at a constant rate. The solution dropping part 20 is connected to the solution blocking area forming part 30 and may be moved in the same direction. A portion of the solution dropping part 20 except for the solution injection port 21 may be formed to be spaced apart from the porous membrane 2.

To this end, the solution dropping unit 20 includes a solution injection port 21, a capillary channel 22, and a solution flow delay unit 23.

The solution injection hole 21 may be recessed from the upper surface of the solution dropping part 20 and formed through the inner space, which is a through hole of the display part 13. Accordingly, when the cleaning solution or the amplification solution is dropped into the solution injection port 21, the cleaning solution or the amplification solution is a hole through which the solution injection port 21 can be injected into the through hole formed so that the solution injection port 21 is above the porous membrane 2.

The sample injection hole 12 may be recessed from the upper surface of the cover part 11a and formed through the inner space. Therefore, when the sample is injected into the sample inlet 12, the sample can flow into the inner space, and the sample penetrates into the sample pad 6 disposed below the sample inlet 12 and is a conjugate pad by chromatography. It moves to the measurement area 3 of the porous membrane 2 via (6).

The capillary channel 22 is a capillary tube that is recessed from the side of the hole forming the solution inlet 21, and when an excess solution is injected into the solution inlet 21, a part of the capillary channel 22 is moved to the capillary channel 22. .

The solution flow delay unit 23 performs a function of slowly transferring the injected solution to the porous membrane 2, and includes a mesh, an absorption pad, a porous membrane, and a filter membrane. ), etc. may be used.

27 is a structural diagram of an immunochromatographic sensor strip according to another embodiment of the present invention, and FIG. 28 is a diagram illustrating a state in which a solution overflows in a porous membrane.

Referring to FIG. 27, in the sensor strip 1, an absorbing layer 9 is additionally laminated under the polymer film 4b. The polymer film 4b and the adhesive layer 4a are spaced apart between the absorbent layer 9 and the porous membrane 2, and the absorbent layer 9 functions to absorb the dripping solution. In addition, one end 7b of the absorbent pad 7 and one end 9b of the absorbent layer 9 may be stacked on each other. The solution absorbed by the absorbent pad furnace 7 is connected to each other by the ends 7b and 9b on one side of the stacked side, so that the solution may be absorbed into the absorbent layer 9. When an excessive amount of a solution equal to or greater than the absorption capacity of the absorbent pad 7 flows from the porous membrane 2, the excess solution is absorbed into the stacked absorbent layer 9. When the solution moves through the porous membrane 2 by a capillary phenomenon, the solution is not absorbed by the separated absorbing layer 9, whereas the solution overflows from the porous membrane 2 as shown in FIG. 28B. When over flow, the overflowed solution may be absorbed by the absorbent layer 9. The absorbent layer 9 may be laminated to the polymer film 4b through an adhesive layer 9a. The width of the absorbent layer 9 may be wider or narrower than the width of the porous membrane 2, and is preferably the same as the width of the porous membrane 2. Because in the manufacturing process of the sensor 1, the absorbent pad 7, the porous membrane 2, the conjugate pad 5, and the sample pad 6 are stacked and then cut to a certain width. The width of the absorbent layer 9 stacked on (4b) is the same as the width of the porous membrane 2.

In addition, the length of the absorbent layer 9 should at least include the measurement area 3 of the porous membrane 2, more preferably, the measurement area 3 and the solution blocking area. Preferably, the entire porous membrane 2 should be included, and more preferably, a portion of the absorbent pad 7 and a portion of the conjugate pad 5 are preferably included. More preferably, it is preferable to include part of the absorbent pad 7 and the sample pad 6.

Claims (10)

delete The immunochromatography sensor strip (hereinafter referred to as'strip') includes a cover portion having a display portion formed therethrough to expose the measurement area to the outside, and a support portion receiving the strip on the upper surface, but the cover portion or the support portion In an immunochromatography sensor cartridge comprising a solution blocking region forming unit for forming a blocking region by pressing or removing a predetermined region of the porous membrane adjacent to the measurement region exposed by the display unit by external pressure, the solution blocking region forming unit:
A guiding part made of a flexible member having one end connected to one side of the through hole forming the display part and extending from one end in the direction of the length axis of the strip (hereinafter referred to as'X-axis'),
A pressing part formed from the other end of the guiding part based on the vertical axis of the strip plane (hereinafter referred to as'Z-axis'),
An immunochromatography sensor cartridge comprising a contact portion protruding downward from the other end of the guiding portion with respect to the Z axis.
The immunochromatography sensor strip (hereinafter referred to as'strip') includes a cover portion having a display portion formed therethrough to expose the measurement area to the outside, and a support portion receiving the strip on the upper surface, but the cover portion or the support portion An immunochromatography sensor cartridge comprising a solution blocking region forming unit for forming a blocking region by pressing or removing a predetermined region of a porous membrane adjacent to a measurement region exposed by the display unit by external pressure,
At least one of the predetermined regions on one side of the through hole forming the display unit and the other side facing the side is depressed to form a passage in the width direction of the strip (hereinafter referred to as'Y-axis direction'),
The solution blocking region forming part:
A guiding part that extends horizontally from one end to the other end, is accommodated in the passage, and moves in the Y-axis direction,
A pressing part formed on the Z-axis basis from the other end of the guiding part or a predetermined point between one end and the other end,
An immunochromatography sensor cartridge, comprising: a contact portion protruding downward from the other end of the guiding portion or from a predetermined point between one end and the other end with respect to the Z axis.
The immunochromatography sensor strip (hereinafter referred to as'strip') includes a cover portion having a display portion formed therethrough to expose the measurement area to the outside, and a support portion receiving the strip on the upper surface, but the cover portion or the support portion In an immunochromatography sensor cartridge comprising a solution blocking region forming unit for forming a blocking region by pressing or removing a predetermined region of the porous membrane adjacent to the measurement region exposed by the display unit by external pressure, the solution blocking region forming unit:
A support part extending in the Y-axis direction from one side of the through hole forming the display part, and connected to the other side facing one side, and
One end is a pressing portion, and the other end includes a pressing portion that is a contact portion,
An immunochromatography sensor cartridge, characterized in that the pressing part is fastened with the support part, and the pressing part is rotated around a point where the pressing part is fastened with the support part.
The immunochromatography sensor strip (hereinafter referred to as'strip') includes a cover portion having a display portion formed therethrough to expose the measurement area to the outside, and a supporting portion for receiving the strip on the upper surface, but the cover portion or the supporting portion In the immunochromatography sensor cartridge comprising a solution blocking region forming unit for forming a blocking region by pressing or removing a predetermined region of the porous membrane adjacent to the measurement region exposed by the display unit by external pressure, the solution blocking region forming unit:
A support part extending in the Y-axis direction from one side of the through hole forming the display part, and connected to the other side facing one side, and
A guiding part formed in the form of a rod extending from one end to the other end and fixed to one side of the through hole, wherein one end and the other end are fixed at different positions with respect to the Z axis,
One end is a pressing portion, and the other end includes a pressing portion that is a contact portion,
Immunochromatography sensor cartridge, characterized in that the pressing portion is moved along the guiding portion.
The immunochromatography sensor strip (hereinafter referred to as'strip') includes a cover portion having a display portion formed therethrough to expose the measurement area to the outside, and a supporting portion for receiving the strip on the upper surface, but the cover portion or the supporting portion An immunochromatography sensor cartridge comprising a solution blocking region forming unit for forming a blocking region by pressing or removing a predetermined region of a porous membrane adjacent to a measurement region exposed by the display unit by external pressure,
The lower surface of the cover part and the upper surface of the support part are connected, and a bending line is formed in the center so that the lower surface of the cover part faces the upper surface of the support part, and is folded along the bending line,
On the cover or base
At least a pair of slits formed in parallel adjacent to the display portion are provided,
The solution blocking area forming part
Immunochromatography sensor cartridge, characterized in that it is moved through between the slits to form a contact portion in contact with the upper surface of the strip porous membrane.
The method according to any one of claims 2 to 6,
The sample is moved from one end to the other in the porous membrane,
Contact
Immunochromatography sensor cartridge, characterized in that located on the upper portion of the region between the measurement region and one end of the porous membrane.
The method according to any one of claims 2 to 6,
The sample is moved from one end to the other in the porous membrane,
Contact
Immunochromatography sensor cartridge, characterized in that located above the region between the measurement region and the other end of the porous membrane.
The method according to any one of claims 2 to 5, wherein the solution blocking region is
The solution droplets are connected and formed,
Solution dripping
An immunochromatography sensor cartridge, characterized in that a solution injection hole is formed through the upper surface of the porous membrane by being embedded from the upper surface of the solution droplet.
The method according to any one of claims 2 to 6, wherein the strip is
An immunochromatography sensor cartridge, characterized in that an absorption layer is further formed at the bottom of the porous membrane to absorb the solution dripping on the porous membrane.

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