KR101965594B1 - Composition for prevention of losing hair or promotion of growing hair comprising bean extract - Google Patents

Abstract

The present invention discloses a composition for promoting hair loss prevention and hair growth and for promoting hair health comprising soybean extract extracted with a C ₁ to C ₅ alcohol having a concentration of 1% to 70% (v / v) as an active ingredient.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing hair loss or promoting hair growth comprising a soybean extract,

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising a soybean extract.

Most animals fall into hairs all at once and new hairy molasses, with about 100 of them hairs per day of about 100,000 hairs in total, and about 100 hairs per day are kept fresh each year .

The hair cycle can be roughly classified into three periods: growth period, regressor period, and rest period. Among them, anagen is a period when active division of cells occurs in the dermal papilla and the growth of new hair is promoted. During this period, the hair grows. Each hair has a growth period of approximately 3 to 5 years for males and 4 to 6 years for females, and as a whole, approximately 80 to 85% of hair corresponds to growth. The catagen is about 3 to 4 weeks when cell division is gradually stopped. Finally, the taloge is a period when the hair is separated from the capillaries and is simply put on the scalp. Approximately three months, and the hair in the rest period is usually easily removed by physical stimuli.

The present invention provides a composition for preventing hair loss or for promoting hair growth and for improving hair health.

One aspect of the present invention provides a composition for promoting hair loss prevention or hair growth comprising an extract of soybeans extracted with a C ₁ to C ₅ alcohol having a concentration of 1 to 70% (v / v) as an active ingredient.

Another aspect of the present invention provides a composition for promoting hair health comprising a soybean extract extracted with a C ₁ to C ₅ alcohol having a concentration of 1% to 70% (v / v) as an active ingredient.

Another aspect of the present invention provides a cosmetic, food or pharmaceutical composition comprising the composition.

The composition according to the present invention comprises a soybean extract extracted with a lower alcohol, specifically a lower alcohol at a low concentration, as an active ingredient, thereby improving the blood circulation of the capillary blood vessels, supplying nutrition to the epithelial cells of the hair follicle, stimulating the hair follicle, Lt; / RTI > Therefore, it suppresses the progress of hair loss, promotes hair growth, further prevents aging of hair and scalp, and improves hair health. In addition, in each hair, the effect of promoting hair growth is promoted by promoting the transition from the resting period to the growing period.

FIG. 1 is a photograph showing the degree of hair growth in a mouse according to application of a soybean extract. FIG.

As used herein, the term " hair loss " means prevention of hair loss and prevention of hair loss as described above, and " hair growth promotion "Refers to promoting the creation of new hair as well as allowing existing hair to grow healthily.

Hair growth is influenced by various factors such as temperature or sunlight, the nutritional status of the individual, the presence or absence of the disease, the presence of hormones, delivery, and exposure to radiation or various drugs. The main factors of hair loss are physiological internal factors and external factors. Physiological aspects of the body include testosterone, a type of male hormone (androgen), and 5-alpha reductase, which converts testosterone into DHT (dihydrotestosterone) to increase secretion of sebum For example). Outside factors due to the external environment include lack of nutritional supply to the hair follicles due to vascular atrophy caused by a lack of blood circulation, and scalp drying. On the basis of this, in order to prevent hair loss and promote hair growth, it is necessary to expand the blood vessels so that the nutrients are supplied to the hair follicles, or to supply directly the hair follicles with the components having the same action as the hair components, or by using a detergent or an anti- To remove excess sebum in the hair follicles, or to prevent drying of the scalp using a moisturizing agent.

It is known that vitamin E, unsaturated fatty acids or anthocyanins contained in soybeans, such as black soybeans, can expand the blood vessels and thus smooth blood circulation. So far, studies on soybean have mainly been conducted in terms of methods for separating and purifying an active ingredient exhibiting pharmacological activity, and research on the medicinal use of soybean itself has hardly been conducted. Also, in the extraction method, an extraction method using an organic solvent having a high concentration, which is an extraction method usually used for natural products, has been mainly used. There are many unknown ingredients in the soybean extract, in addition to the known ingredients, some of which may have pharmacological effects useful in the human body. In addition, the useful components may not be extracted by a conventional extraction method using a high concentration organic solvent. The inventors of the present invention have found that extracts obtained from soybeans by using a low concentration of organic solvent as an extraction solvent are devoid of recognition of the extraction method commonly used in extracting natural products and that the extracts are more effective than the extracts extracted with high concentration organic solvents, Promoting and hair health promoting effect.

Accordingly, one aspect of the present invention is to provide a composition for hair containing, as an active ingredient, a soybean extract extracted with a C ₁ to C ₅ alcohol having a concentration of 1 to 70% (v / v), specifically a composition for promoting hair loss prevention or hair growth, Thereby providing a composition for promotion. The soybean extract has the effect of promoting the activation of the dermal papilla cells and promoting the proliferation and expanding the capillary blood vessels, thereby activating the blood circulation and supplying nutrition to the hair follicle. It also promotes the transition to growth period for the hair of the resting period. Therefore, the composition according to the present invention containing the active ingredient as an active ingredient can prevent hair loss, accelerate hair growth, prevent aging of hair, increase hair shine, and enhance hair health by strengthening hair root.

In one aspect of the present invention, the composition is characterized by comprising a soybean extract extracted using a low-concentration organic solvent as an active ingredient. In another aspect of the present invention, the organic solvent comprises a C ₁ to C ₅ alcohol. The C ₁ to C ₅ alcohols may be any one or a mixture of two or more selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol, Ethanol, but is not limited thereto. In another aspect of the present invention, the concentration of the C ₁ to C ₅ alcohol is 1 to 70% (v / v), specifically 1 to 40% (v / v), more specifically 5 to 30% v / v), and even more specifically 12 to 25% (v / v).

In one aspect of the present invention, the soybean contains all of the soybean extracts having the effect of preventing hair loss, accelerating hair growth, or improving hair health. In another aspect of the invention, the beans comprise black beans or colored beans. In another aspect of the present invention, the soybean is selected from the group consisting of Seolite, Seomyeutae, Blackcurrant, Chrysanthemum, Fuda bean, Kidney bean, Stark bean, Fence bean, Mung bean, Red bean, And includes at least one selected.

As used herein, the term " black bean " refers to soybeans having a black color in the color of the grain shell. Examples of the black beans include, but are not limited to, seaweed, seomyeotae, and black soybeans. The name of black bean can be variously called by region, classification, influence of dialect. As used herein, the term "black bean extract" refers to a substance extracted from black beans through various extraction methods, and includes, for example, a substance extracted using an organic solvent or the like, various fractions of the extracted substance, and the like .

As used herein, the term "colored-bean" refers to soybeans having a dense color of grain shells, and includes not only black but also beans having a color such as red, yellow or blue. Examples of the above-mentioned colored beans include, but are not limited to, Seoritae, Seomotei, Black tea, Yellowtail, Fuda bean, Kidney bean, Stain kidney bean, Fence bean, Mung bean, Red bean, Reed, Soybean bean, Soybean bean or Soybean . The names of colored beans can be variously called by region, classification, and the influence of dialects. In the present specification, the term "colored bean extract" refers to a substance extracted from colored beans through various extraction methods, and includes, for example, a substance extracted using an organic solvent or the like, various fractions of the extracted substance, and the like .

The composition according to one aspect of the present invention comprises from 0.001 to 90% by weight, specifically from 1.0 to 70% by weight, more particularly from 5.0 to 50% by weight, of soybean extract, based on the total weight of the composition. When it is contained in the above weight percentage, it is not only suitable for exhibiting the intended effect of the present invention but also can satisfy both the stability and safety of the composition, and may be suitably included in the above range in terms of cost effectiveness.

One aspect of the present invention provides a cosmetic composition comprising a composition comprising a soybean extract extracted with a C ₁ to C ₅ alcohol at a concentration of 1% to 70% (v / v).

The cosmetic composition may be provided in any formulation suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition may contain, in addition to the above-mentioned substances, other ingredients which can give rise to a synergistic effect on the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may comprise a material selected from the group consisting of vitamins, high molecular peptides, high molecular weight polysaccharides and sphingolipids. The cosmetic composition according to the present invention may also contain a moisturizing agent, an emollient agent, a surfactant, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjusting agent, an organic and inorganic pigment, a fragrance, . The blending amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The blending amount thereof can be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition have.

One aspect of the invention provides a food composition comprising a composition comprising a soybean extract extracted with a C ₁ to C ₅ alcohol at a concentration of 1% to 70% (v / v).

The formulation of the food composition is not particularly limited, but may be formulated into tablets, granules, drinks, caramels, and the like. The food composition of each formulation may be formulated by the person skilled in the art without difficulty, depending on the purpose of formulation or use, in addition to the active ingredient, and the synergistic effect may occur when the composition is applied simultaneously with other ingredients.

The dosage of the active ingredient is within the level of those skilled in the art and its daily dose is, for example, from 0.1 mg / kg / day to 5000 mg / kg / day, more specifically from 50 mg / kg / day, but it is not limited thereto, and may be varied depending on various factors such as the age, health condition, and complications of the subject to be administered.

One aspect of the present invention provides a pharmaceutical composition comprising a composition comprising a soybean extract extracted with a C ₁ to C ₅ alcohol at a concentration of 1% to 70% (v / v). In another aspect of the present invention, the pharmaceutical composition may exhibit an effect of preventing or treating hair loss, promoting hair growth, and enhancing hair health.

The pharmaceutical composition according to one aspect of the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like. Formulations for oral administration may be in the form of tablets, pills, soft and hard capsules, granules, powders, granules, solutions, emulsions or pellets, It is not. Formulations for parenteral administration may be, but are not limited to, solutions, suspensions, emulsions, gels, injections, drops, suppositories, patches or spray formers. The formulations may be readily prepared according to conventional methods in the art and may be prepared by conventional means including, but not limited to, surfactants, excipients, wetting agents, emulsifying accelerators, suspending agents, salts or buffers for controlling osmotic pressure, colorants, spices, stabilizers, preservatives, Other commonly used adjuvants may be used.

The effective ingredients of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 5000 mg / kg / day, more specifically from 50 mg / kg / day to 500 mg / / Day, but is not limited thereto.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples, comparative examples and test examples. However, these examples, comparative examples and test examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

[Examples 1 to 4] Preparation of 10%, 20%, 50% and 70% ethanol solids extracts

1 kg of the dried seaweed was immersed in 10 L of ethanol solutions of 10%, 20%, 50% and 70% (v / v) at a temperature of 50 캜, refluxed for 3 hours for 5 hours, and left at room temperature for 12 hours . The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.

[Example 5] Preparation of 20% ethanol Seomyeo extract

1 kg of the dried seedlings were immersed in 5 L of a 20% (v / v) aqueous ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was kept at low temperature until use.

[Example 6] Preparation of 20% ethanolic kidney bean extract

300 g of dried green beans was immersed in 1.5 L of a 20% (v / v) aqueous ethanol solution at a temperature of 60 ° C, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use

[Example 7] Preparation of 20% ethanol stain kidney bean extract

300 g dried bean sprouts were immersed in 1.5 L of a 20% (v / v) aqueous ethanol solution at a temperature of 60 ° C., refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was kept at low temperature until use.

[Example 8] Preparation of 20% ethanol bean soybean extract

300 g of dried bean sprouts beans were immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 ° C, refluxed for 3 hours, and left at room temperature for a certain time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was kept at low temperature until use.

[Example 9] Preparation of 20% ethanol extract

1 kg of the dried persimmon was immersed in 5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed twice for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored frozen until use.

[Example 10] Preparation of 20% ethanol furry soybean extract

300 g of dried fleabane beans were immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored frozen until use.

[Example 11] Preparation of 20% ethanol fence soybean extract

1 kg of dried fence beans was immersed in 5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.

[Example 12] Preparation of 20% ethanol soybean extract

1 kg of dried soybeans was immersed in 5 L of a 20% (v / v) ethanol solution at a temperature of 60 ° C., refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.

[Example 13] Preparation of 20% ethanol sugar extract

300 g of the dried reed was immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15%, and the prepared powders were refrigerated until use.

[Example 14] Preparation of 20% ethanolic soybean curd extract

300 g of dried red oak was immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.

[Example 15] Preparation of 20% ethanol black tea extract

300 g of the dried black tea was immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.

[Example 16] Preparation of a frosted butanol extract

25 g of the 20% (v / v) ethanol solids extract obtained in Example 2 was dissolved in 250 ml of distilled water and extracted twice with 250 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and then lyophilized to prepare a sample Respectively. The yield was 5 ~ 15%, and the prepared samples were stored frozen until use.

[Example 17] Preparation of Butanol Extract of Seomyeongbuk

1 g of the 20% (v / v) ethanol ethanol extract obtained in Example 5 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and lyophilized to prepare a sample Respectively. The yield was 5 ~ 15%, and the prepared samples were stored frozen until use.

[Example 18] Preparation of bean butanol extract of fence

25 g of the 20% (v / v) ethanol fence soybean extract obtained in Example 11 was dissolved in 250 ml of distilled water and extracted twice with 250 ml of n-butanol using a separating funnel. The obtained butanol layer was concentrated under reduced pressure, . The yield was 5 ~ 15%, and the prepared samples were stored frozen until use.

[Example 19] Production of bean butanol extract of furundae

1 g of the 20% (v / v) ethanol furda soybean extract obtained in Example 10 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and freeze- . The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.

[Example 20] Preparation of Butanol Extract from Huang Tta

1 g of the 20% (v / v) ethanol extract obtained in Example 9 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and lyophilized to prepare a sample Respectively. The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.

[Example 21] Preparation of bean sprout soybean butanol extract

1 g of the 20% (v / v) ethanol bean soybean extract obtained in Example 8 was dissolved in 10 ml of distilled water, and the mixture was extracted twice with 10 ml of n-butanol using a separating funnel. The obtained butanol layer was concentrated under reduced pressure, . The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.

[Example 22] Preparation of soybean butanol extract

25 g of the 20% (v / v) ethanol soybean extract obtained in Example 12 was dissolved in 250 ml of distilled water and extracted twice with 250 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and lyophilized to prepare a sample Respectively. The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.

[Example 23] Preparation of butanol extract of kidney bean

1 g of the 20% (v / v) ethanolic bean extract obtained in Example 6 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and then lyophilized to prepare a sample Respectively. The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.

[Comparative Example 1] Preparation of a sea thyme extract using water

1 kg of the dried seaweed was immersed in 10 L of water at a temperature of 100 캜, refluxed three times for 5 hours, and left at room temperature for 12 hours. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.

[Comparative Example 2] Preparation of a seaweed extract using 75% ethanol

1 kg of the dried seaweed was immersed in 10 L of a 100% (v / v) ethanol solution at a temperature of 50 캜, refluxed for 3 hours for 5 hours, and left at room temperature for 12 hours. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.

[Comparative Example 3] Preparation of a seaweed extract using 100% ethanol

A seared extract was obtained in substantially the same manner as in Comparative Example 2, except that 100% (v / v) ethanol was used. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.

[Test Example 1] Evaluation of promoting effect of proliferation of dermal papilla cells

Rat Vibrissa dermal papillae cell (PVGDPLTT sa) transfected with polyomavirus large T gene encoding temperature-sensitive T antigen into PVG mouse vibrissa dermal papilla cell 6. Hereinafter, it is referred to as a DP cell) to evaluate the promoting effect of the soybean extract on the development of the papilla cell.

DP cells were first prepared to a concentration of about 10 ⁴ cells / well per well in a 96-well plate. Examples 1 to 4 and Comparative Examples 1 to 3 were dissolved in DMSO (dimethyl sulfoxide) or ethanol, and then serial dilution was performed with DMEM medium having a 5% FBS serum concentration to obtain a final concentration of 10 ppm. 200 占 퐇 of the medium to which Examples 1 to 4 and Comparative Examples 1 to 3 were added was added to each well and cultured at 37 占 폚 in a 5% CO ₂ incubator for about 3 days. MTT solution prepared at a concentration of 2 mg / ml was added in an amount of 50 占 퐇 / well, followed by incubation in the same incubator for 4 hours. When it was judged that the MTT reagent was absorbed into the intracellular mitochondria, the supernatant was removed, 150 μl of DMSO was added, and the mixture was stirred for 10 minutes to uniformly dissolve the formazan dye. The OD value at 515 nm was measured using an ELISA reader, and the number of cells was measured according to a standard curve prepared beforehand. Based on the measured cell numbers, the ability of the examples 1 to 4 and the comparative examples 1 to 3 to promote the proliferation of the dermal papilla cells (%) was calculated and the results are shown in the table below.

Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Example 3 Example 4 Dairy cattle
Cell proliferation promoting ability (%) 15.1 ± 1.0 18.8 ± 0.9 12.5 ± 0.4 24.6 ± 0.4 42.1 ± 1.2 28.8 ± 1.3 22.5 ± 1.4

As can be seen from the above, compared with Comparative Example 1 extracted with water and Comparative Examples 2 and 3 extracted with 75% (v / v) and 100% (v / v) . Especially, the effect of promoting the growth of the papilla cells of Example 2, which is a soybean extract extracted with 20% ethanol, was the best. Therefore, the soybean extract extracted with alcohol promotes the proliferation of the papillary cells, thereby preventing hair loss, accelerating hair growth, and further enhancing hair health.

[Test Example 2] Capillary expansion effect evaluation

When the K ⁺ channel is opened, mitogenesis is activated and the vascular smooth muscle relaxes and blood vessels expand. On the other hand, it is known that inhibition of NIH3T3 fibroblast proliferation is inhibited when K ⁺ channel is blocked. Therefore, when the growth of NIH3T3 fibroblasts is promoted by the sample treatment, it can be seen that the K ⁺ channel is opened and further the vasodilation is promoted.

First, NIH3T3 fibroblasts were seeded at a concentration of 10 ⁴ cells / well in a 24-well culture plate and cultured in DMEM medium containing 10% FCS (fetal calf serum) and 2 mM L-glutamine, Time. Then, the culture medium was replaced with the medium to which Examples 1 to 4 and Comparative Examples 1 and 3 were added, respectively, and cultured for 72 hours. The medium preparation methods to which Examples 1 to 4 and Comparative Examples 1 and 3 are added are the same as those of Test Example 1. After the medium was removed, the cells were washed with PBS (Ca ²⁺ / Mg ²⁺ free), treated with 0.5 ml TE (trypsin-ethylenediamine tetraacetic acid), and cultured. DMEM containing 10% FCS was added and cells were taken and centrifuged at 6000 rpm in an EP tube. The supernatant was removed, and the remaining cell lysate was suspended in 100 μl of PBS, and then the number of cells was measured using a hemocytometer. Based on this, the capillary vasodilatability of Examples 1 to 4 and Comparative Examples 1 and 3 was calculated and shown in the table below.

Comparative Example 1 Comparative Example 3 Example 1 Example 2 Example 3 Example 4 Capillary
Extensibility (%) 8.3 ± 1.2 4.3 ± 1.2 12.6 ± 4.4 34.1 ± 2.2 18.8 ± 3.1 22.5 ± 0.7

As can be seen from the above, the capillary vasodilating effect of Examples 1 to 4 was superior to those of Comparative Examples 1 and 3, which were extracted with water and 75% (v / v), respectively. Especially, the capillary vasodilating effect of Example 2, which is a soybean extract extracted with 20% ethanol, was the best. Therefore, the soybean extract extracted with alcohol can improve blood circulation of scalp capillary blood vessels, thereby improving hair health, inhibiting the progress of hair loss and promoting hair growth.

[Test Example 3] Evaluation of hair growth promoting effect

The hair growth promoting effect of the mice was evaluated using Examples 2 and 4. First, the dorsal hairs of the mice (C57BL / 6) at 47 to 53 days of age were removed with an electric clipper. Since the mouse hair of this age is in a resting state, it does not grow immediately. Among them, mice with good back skin were selected and divided into three groups so that 5 to 7 mice were included in each group, and then they were raised in a separate case. The cereal feeds in which Examples 2 and 4 were included at 0.6% were freely taken through diets. The control group was fed with cereal feed containing no soybean extract. After 23 days, the change of the epilation area of each group was observed and the photograph is shown in Fig. In addition, hair of each newly-developed mouse on the hair removal area was collected by electric creepers and weighed. The results are shown in the table below.

Control group Example 2 Example 4 Hair Weight (mg) 32.1 ± 12.2 98.5 ± 8.4 56.1 ± 12.2

As can be seen from the above, the soybean extract extracted with ethanol promoted the growth of mouse hair. This can be seen from the appearance shown in Fig. 1 and the hair weight in Table 3. Especially, soybean extract extracted with 20% ethanol showed excellent hair growth promoting effect. Therefore, the soybean extract extracted with alcohol has excellent hair growth promoting effect.

Formulation examples of the composition are described below, but are not intended to limit the invention, but merely to illustrate them.

[Formulation Example 1] Soft capsule

A soft capsule was prepared by mixing 100 mg of a safflower extract, 50 mg of a soybean extract, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow pearls and 6 mg of lecithin, .

[Formulation Example 2] Tablets

100 mg of fence bean extract, 100 mg of glucose, 96 mg of starch and 4 mg of magnesium stearate are mixed and 40 mg of 30% ethanol is added to form granules. The granules are dried at 60 ° C. and tableted by tabletting.

[Formulation Example 3] Drinking agent

100 mg of Seomunghae extract, 10 g of glucose, 2 g of citric acid and 187.8 g of purified water are mixed and filled into a bottle. The final volume of the contents should be 200 ml.

[Formulation Example 4] Health food

Seaweed extract ......................... 1000 mg

Vitamin mixture

Vitamin A Acetate ...................... 70 ㎍

Vitamin E .............................. 1.0 mg

Vitamin B1 ............................. 0.13 mg

Vitamin B2 ............................ 0.15 mg

Vitamin B6 .............................. 0.5 mg

Vitamin B12 ............................. 0.2 g

Vitamin C ................................ 10 mg

Biotin .................................. 10 μg

Nicotinic acid amide ....................... 1.7 mg

Folic acid .................................... 50 ㎍

Calcium pantothenate .......................... 0.5 mg

Mineral mixture

Ferrous sulfate .............................. 1.75 mg

Zinc oxide ............................... 0.82 mg

Magnesium carbonate ........................... 25.3 mg

Potassium Phosphate .............................. 15 mg

Secondary calcium phosphate .............................. 55 mg

Potassium citrate ............................... 90 mg

Calcium carbonate ................................ 100 mg

Magnesium chloride ............................ 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is relatively mixed with the ingredient suitable for health food, the compounding ratio thereof may be arbitrarily changed, and the above ingredients may be mixed according to the ordinary health food manufacturing method, And can be used in the manufacture of a health food composition according to a conventional method.

[Formulation Example 5] Cream

Creams are prepared by conventional methods according to the compositions listed in the table below.

Compounding ingredient Content (% by weight) Blackberry extract 3.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (6)

A composition for oral hair loss prevention or promotion of hair growth, comprising an extract of a sea tangle extracted with ethanol at a concentration of 12% to 25% (v / v) as an active ingredient.
A composition for oral administration for enhancing hair gloss or strengthening hair roots, which comprises, as an active ingredient, an edible extract extracted with ethanol at a concentration of 12% to 25% (v / v).
3. The method according to claim 1 or 2,
Wherein the composition comprises from 0.001 to 90% by weight of the perfume extract, based on the total weight of the composition.
3. The method according to claim 1 or 2,
The composition improves the blood circulation of the scalp and nourishes the hair follicle.
A food composition comprising the composition according to claims 1 or 2.
6. A pharmaceutical composition for prevention and treatment of hair loss comprising the composition according to any one of claims 1 to 5.

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