KR101936687B1 - Cosmetic compositions as power type - Google Patents

Abstract

The present invention relates to a powder type cosmetic composition, and more particularly, to a powder type cosmetic composition in which vitamin C and a protease are stabilized. The powder type cosmetic composition according to the present invention is useful because it can be used and stored for a long time while maintaining the activity of the protease, and can be used and stored for a long period while maintaining the efficacy of vitamin C.

Description

COSMETIC COMPOSITIONS AS POWER TYPE [0002]

The present invention relates to a powder type cosmetic composition, and more particularly, to a powder type cosmetic composition in which vitamin C and a protease are stabilized.

Vitamin C has a whitening effect, such as improvement of pigmentation such as spots, freckles, and black spots, a wrinkle improving effect such as antioxidant and collagen biosynthesis promotion, and a moisturizing effect. However, vitamin C may be stable in a completely dried crystalline state, but it is unstable in heat and easily destroyed by oxidation in an aqueous solution.

Subtilisin, a protease, is a serine protein hydrolyzing enzyme secreted by Bacillus subtilis. In addition, another protease, papain, is a protein hydrolytic enzyme obtained from crystals in the papaya fruit milk. It is stable at pH 4.0 ~ 8.0 and decomposes the protein (endopeptidase action) to produce peptone. Protein hydrolytic enzymes such as subtilisin and papain can help to whiten the vitamin C by removing old keratin, but its activity can be maintained only when the quaternary structure of the protein is maintained.

Conventionally, the company has sold vitamin C and proteolytic enzymes in the form of liquids such as skins, creams and lotions. However, when stored in the presence of an aqueous solution, the vitamin C is oxidized and discolored, so that the whitening effect can not be sufficiently exhibited, and the activity of the proteolytic enzyme is reduced to exert the exfoliation efficacy.

As a result of intensive efforts to solve the above problems, the present inventors have found that vitamin C can be stabilized by L-cysteine as a reducing agent, stabilize proteolytic enzyme by sugar, maintain its efficacy and potency even when stored for a long period of time So that a powder type cosmetic composition can be obtained.

Korean Patent Publication No. 10-2001-0107962

It is an object of the present invention to provide a powder-type cosmetic composition in which efficacy and potency are maintained even after use for a long period of time after use and storage.

In order to achieve the above object, the present invention provides a powder type cosmetic composition containing a proteolytic enzyme and a sugar.

In addition, the present invention relates to a method for preparing a pharmaceutical composition, which comprises lyophilizing vitamin C and L-cysteine;

Lyophilizing proteolytic enzymes and sugars; And

And lyophilizing the lyophilized material obtained by the lyophilization to obtain a powder type cosmetic composition.

The powder type cosmetic composition according to the present invention is useful because it can be used and stored for a long time while maintaining the activity of the protease, and can be used and stored for a long period while maintaining the efficacy of vitamin C.

1 shows a process for producing a powder type cosmetic composition.

The present invention relates to a powder type cosmetic composition containing a proteolytic enzyme and a saccharide in one aspect.

In the powder type cosmetic composition according to one aspect of the present invention, the composition may further comprise vitamin C, and may further comprise L-cysteine.

In the powder type cosmetic composition according to one aspect of the present invention, the composition contains vitamin C and L-cysteine in the form of powder, thereby preventing the oxidation of vitamin C in a high temperature and wet environment. The whitening effect can be kept constant. On the other hand, in the powder type cosmetic composition which is one aspect of the present invention, the proteolytic enzyme can maintain the quaternary structure by the rich hydroxy group present in the sugar. In the powder form, the hydroxy group of the sugar also provides a hydrophilic environment around the protease to maintain the quaternary structure of the protease to maintain the function of the enzyme. Therefore, when lyophilized protease is used in powder form together with saccharides, the structure and activity of protein can be well preserved in a high temperature and humid environment.

In the powder type cosmetic composition according to one aspect of the present invention, the protease may include one or more substances selected from the group consisting of subtilisin, papain, and keratinase.

Subtilisin is a serine protein hydrolytic enzyme secreted by Bacillus subtilis, which is effective in exfoliating and is an optimal protease in neutral or weakly alkaline pH environment. Papain is an enzyme that breaks down proteins to produce peptones. On the other hand, keratinease is keratolytic enzyme.

In the powder type cosmetic composition according to one aspect of the present invention, the sugar may include at least one substance selected from the group consisting of dextrin and trehalose.

In the powder type cosmetic composition according to one aspect of the present invention, the composition may contain vitamin C and L-cysteine at a weight ratio of 60% or more based on the total weight of the cosmetic composition. When vitamin C and L-cysteine were contained in an amount of less than 60%, it was confirmed that the effect of vitamin C was hardly observed. According to the above-mentioned viewpoint, the content of vitamin C and L-cysteine can be 65% or more, 70% or more, 75% or 80% or more. In addition, in the powder type cosmetic composition as one aspect of the present invention, the proteolytic enzyme and sugar contained in the composition may be contained at a weight ratio of less than 40% based on the total weight of the cosmetic composition. It was confirmed that the content of vitamin C and L-cysteine was lowered and the effect of vitamin C was not observed sufficiently when protease and saccharide were contained in a proportion of 40% or more. By such a viewpoint, the content of protease and sugar can be less than 35%, less than 30%, less than 25% or less than 20%.

In the powder type cosmetic composition according to one aspect of the present invention, the composition may contain vitamin C to L-cysteine at a weight ratio of 250 to 1: 1. When vitamin C to L-cysteine is contained in a ratio of less than 1: 1, whitening effect by vitamin C was observed to be insignificant. On the other hand, when the content of vitamin C is larger than 250: 1, it is confirmed that the L-cysteine weight ratio is too small to prevent the oxidation reaction of vitamin C. From the above viewpoint, the vitamin C to L-cysteine may be contained at a weight ratio of 200 to 1: 1, 150 to 2: 1, 100 to 3: 1 or 50 to 5: 1.

On the other hand, in the powder type cosmetic composition according to one aspect of the present invention, the composition may contain a protease at a weight ratio of 20 to 0.1: 1. When the protease was contained in an amount of less than 0.1: 1, the content of protease was small and the keratinolysis was observed to be insignificant. On the other hand, it was confirmed that the protease activity was not maintained when the protease was contained more than 20: 1. In view of the above, the protease may be contained at a weight ratio of 15 to 0.2: 1, 10 to 0.3: 1, 5 to 0.4: 1 or 5 to 0.5: 1.

In the powder type cosmetic composition according to one aspect of the present invention, vitamin C and L-cysteine as a reducing agent can be lyophilized together to increase the stability of the powder itself, and the proteinase is stabilized by the sugar under high temperature and high humidity, It is possible to prevent deterioration of the quality of the product and to maintain the efficacy for a long period of time. In addition, a specific component can be selectively added to existing products so that the easily deteriorated substances in an aqueous solution can be delivered to consumers in a stable state, and can be used in accordance with consumer's preferences.

In the powder type cosmetic composition according to one aspect of the present invention, the composition may be one for exfoliating. In addition, in the powder type cosmetic composition which is one aspect of the present invention, the composition may contain whitening agents.

The present invention, in another aspect,

Lyophilizing vitamin C and L-cysteine;

Lyophilizing proteolytic enzymes and sugars; And

And lyophilizing the lyophilized material obtained by the lyophilization.

In one aspect of the present invention, the method comprises: dissolving vitamin C and L-cysteine in distilled water, followed by lyophilization; Dissolving proteinase and sugar in distilled water, followed by lyophilization; And lyophilized products of vitamin C and L-cysteine (L-cystein) obtained by the lyophilization; And a lyophilizate of a protease and a sugar.

In another aspect of the present invention, the protease may include at least one enzyme selected from the group consisting of subtilisin and papain.

In another embodiment of the present invention, the sugar may be at least one sugar selected from the group consisting of dextrin and trehalose.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[ Example  One] Cosmetics  Preparation of composition

At room temperature, powdered vitamin C and L-cysteine are dissolved in distilled water and lyophilized. In addition, the powdered subtilisin, papain enzyme, dextrin and trehalose are dissolved in distilled water at room temperature, lyophilized, and then the lyophilized powder is crushed finely and filtered with a mesh. Each of the powders filtered and dried is lyophilized in a container which is covered with a mesh by covering the contents with silica so that the silica capable of absorbing the internal moisture can be 10% or more of the total content weight , 80% or more of vitamin C and L-cysteine, and subtilisin, papain enzyme, dextrin and trehalose in an amount of less than 20%. The manufacturing process of Example 1 is shown in Fig.

The proportion of the cosmetic composition is shown in Table 1 below.

Ingredients Content ratio (%) Vitamin C 87.0 L-cysteine 3.0 Papain 3.5 Subtilisin 3.5 Trehalose 1.5 dextrin 1.5 total 100.0

[ Example  2] Evaluation of Stability of Powders Containing Proteolytic Enzymes and Sugars

In the powdery form of proteolytic enzyme and sugar, it was observed that the titer was maintained. Subtilisin or papain was used as a protease, and trehalose and dextrin were used as a sugar. The powders used in the comparative examples and the experimental examples were prepared by dissolving powdery proteases (Comparative Experiments 1 and 2) or proteolytic enzymes and sugars (Experimental Examples 1-4) in distilled water at room temperature, And each powder was crushed finely and filtered by a mesh. In order to measure the activity after 7 days from the initial stage, each of the powders was stored for 7 days in a thermo-hygrostat (relative humidity 80%, temperature 45) for measuring the activity.

(1) titration of subtilisin

Each of the powders was dissolved in a 50 mM Tris-HCl (pH 8.0) buffer (37 ° C) using 0.75 mM N-acetyl-L-tyrosine ethyl ester (ATEE) as a substrate and the absorbance was measured at 337 nm To measure the amount of substrate decomposition.

Enzyme type Enzyme content (%) Sugar content (%)
(Trehalose + dextrin) 7 days after initial conversion Comparative Experimental Example 1 Subtilisin 100% 0% 91% Experimental Example 1 Subtilisin 70% 30% 95% Experimental Example 2 Subtilisin 50% 50% 96%

Experiments 1 and 2, in which sugar was present together with Comparative Example 1 in which only subtilisin was present, were confirmed to maintain high titers even after 7 days from the production of the powder.

(2) Measurement of papain's activity

Each powder was dissolved in 25 mM sodium phosphate buffer (37 ° C) using N-α-benzoyl-DL-Arg β-naphthylamide (BANA) as a substrate. After a certain period of time, 2% . 0.06% p-dimethylaminocinnamaldehyde ethanol solution was added as a coloring agent and absorbance was measured at 540 nm to measure the substrate decomposition amount.

Enzyme type Enzyme content (%) Sugar content (%)
(Trehalose + dextrin) 7 days after initial conversion Comparative Experimental Example 2 Papain 100% 0% 85% Experimental Example 3 Papain 70% 30% 92% Experimental Example 4 Papain 50% 50% 95%

Experimental Example 2, in which sugar was present together with Comparative Example 2 containing papain alone, was confirmed to maintain high titer even after 7 days of powder production. In addition, the titer of subtilisin was higher than that of papain.

[ Example  3] Cosmetics  Evaluation of composition stability

The powder used in each of Comparative Experimental Examples and Experimental Examples was prepared by dissolving powdered vitamin C (Comparative Example 3) or vitamin C, proteolytic enzyme and saccharide (Experimental Examples 5 and 6) in distilled water at room temperature, And each powder was crushed finely and filtered by a mesh. Each powder was placed in a sealed container with no shading and airtightness, and stored for 2 weeks in a thermo-hygrostat (relative humidity 80%, temperature 45) for discoloration.

Vitamin C
content(%) L-cysteine content (%) Papain + subtilisin
(1: 1) Content (%) Dextrin + trehalose
(1: 1) Content (%) Discoloration after 2 weeks storage Comparative Experimental Example 3 90.0% 0% 7.0% 3.0% discoloration Experimental Example 5 87.0% 3.0% 7.0% 3.0% stability Experimental Example 6 80.0% 10.0% 7.0% 3.0% stability

Comparative Example 3 containing only vitamin C powder showed discoloration when stored for a long period of time. However, in Experimental Examples 5 and 6 in which L-cysteine and vitamin C were mixed, it was confirmed that they were stably stored without discoloration of vitamin C.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (14)

An additive composition for maintaining the activity of a protease,
The additive composition for potency of the protease comprises sugar as an active ingredient,
And is added to a powder type cosmetic composition containing protease, vitamin C, and L-cysteine,
Wherein the proteolytic enzyme and the saccharide are contained in a weight ratio of 10% or more and less than 40% based on the total weight of the cosmetic composition,
Wherein the vitamin C and L-cysteine are contained in a weight ratio of 60% or more and less than 90% with respect to the total weight of the cosmetic composition. delete delete The composition according to claim 1, wherein the protease comprises at least one enzyme selected from the group consisting of subtilisin, papain, and keratinase. . The composition of claim 1, wherein the sugar comprises at least one sugar selected from the group consisting of dextrin and trehalose. delete delete The composition of claim 1, wherein the cosmetic composition comprises vitamin C to L-cysteine at a weight ratio of 250 to 1: 1. The additive composition according to claim 1, wherein the cosmetic composition contains a protease at a weight ratio of 20 to 0.1: 1. The composition of claim 1, wherein the cosmetic composition is for exfoliation. The additive composition according to claim 1, wherein the cosmetic composition is whitening-resistant. delete delete delete

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