KR101917712B1 - Cosmetic composition comprising extract of azadirachta indica and paeonia lactiflora by ultrasonification and black yeast fermentation - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing comprising extracts of Azadirachta indica and Paeonia lactiflora by ultrasonification and black yeast fermentation. Specifically, the present invention provides a cosmetic composition which has excellent skin moisturizing, skin barrier improvement, and antioxidation effects by extracting Azadirachta indica and Paeonia lactiflora using ultrasonic extraction and fermenting the same using black yeast.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a cosmetic composition containing an extract of Angelica keiskei koidz. And a black yeast fermented extract,

The present invention relates to a cosmetic composition comprising an ultrasonic treatment of nymphenicum and a peony root and fermented black yeast extract as an active ingredient. The cosmetic composition has a moisturizing effect and a skin barrier improving effect.

The goal of cosmetics is to maintain beautiful and healthy skin, and also to delay the aging of naturally occurring skin as it gets older. In general, aging is caused by oxidative damage of biomaterials by free radicals. That is, reactive radicals such as active oxygen such as hydrogen peroxide, hydroxy radical, and superoxide radical peroxidize the lipid constituting the cell membrane to destroy the cell membrane, oxidize the protein, attack the nucleic acid and cause oxidative damage, Destroys and eventually kills cells. Skin characteristics that are most noticeable due to aging include wrinkles, decreased elasticity, and skin dullness. In addition to wrinkles and elasticity decline, after 40s, facial color is not clear and is often gray and dark.

Especially for young women, when they experience childbirth, they have eosinophilia, blood circulation disorder, which causes their metabolic ability to fall and their skin to become rough and rough. When the metabolism ability is lowered and the skin becomes rough, the water content is also easily reduced. Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer to about 10 to 30% in the keratin layer. The keratinocyte layer is known to have an important role in the moisturization of the skin due to the existence of a substance having a hydrophilic water retention capacity called a natural moisturizing factor (NMF). The normal keratinocyte layer is maintained at a moisture content of 10 to 30%, and the skin is smooth and soft, and the body protection functions normally.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important for preventing skin aging. However, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, There is a problem that the emulsion stability of the cosmetic is impaired due to the strong electrolyte. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize. The ability to retain the moisture of the stratum corneum can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea and inorganic salts. And the development of a substance with high moisturizing effect is one of the important research subjects in cosmetics. Materials known to have antioxidant activity include ascorbic acid, dl-a-tocopherol, beta-carotene, dibutylhydroxyltoluene (BHT) and the like However, since these materials are used as a synthetic product, there is an urgent need to develop new antioxidative cosmetics that can be applied to skin safely and usefully, because they may cause problems of skin safety when used for a long time or in a large amount.

Therefore, studies on skin aging and moisturizing improvement are expected to be continuously carried out. Many cosmetic products using natural products are being developed in order to solve such skin problems and to reduce skin irritation caused by various chemicals. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials. For this purpose, researches are being actively carried out to develop materials having excellent safety and skin beauty effect by searching skin physiological activity of various natural vegetable persons.

Japanese Laid-Open Patent Application No. 2009-13128 discloses an external preparation for skin and oral composition containing oily extracts of plants extracted using extraction media such as sesame oil, milk, palm oil, beeswax, and the like. The oily plant extract has various effects.

Korean Patent Registration No. 10-0628651 discloses a cosmetic composition for skin whitening comprising an upper body extract and a nematode extract as an active ingredient.

The present inventors have studied the applicability of various natural products to cosmetics. As a result, it has been found that the extracts prepared by fermenting the ultrasonic extract of nimabi and peony root with mycelia of black yeast are effective in skin moisturizing, skin soothing, antioxidant effect, skin barrier improvement And to provide a cosmetic composition containing the same.

The above-mentioned problem is achieved by a cosmetic composition for skin moisturizing comprising an ultrasonic treatment of nimabi and peony and an extract of fermented black yeast.

Preferably, the cosmetic composition has a skin barrier improving effect.

Also, preferably, the ultrasonic treatment of the nimabi and peony and the black yeast fermentation extract are carried out by mixing nimabi and peonies with purified water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3- The mixture is placed in an extraction solvent selected from the group consisting of propylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane and mixtures thereof, and the mixture is extracted with an ultrasonic extraction device for about 1 to 12 hours and then fermented with black yeast .

Preferably, the ultrasonic treatment and black yeast fermentation extract of nimabi and peony root extracts are prepared by dissolving nymphenicol and peony root extracts in an amount of 1 to 7 days at a temperature of 20 to 40 캜, Lt; / RTI > during fermentation.

Also, preferably, the ultrasonic treatment of nimabi and peony and the black yeast fermentation extract may be contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may also be selected from the group consisting of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, Any one of the formulations may be used.

The cosmetic composition of the present invention contains, as an active ingredient, an ultrasonic treatment of nimabi and peony, and a black yeast fermented extract which are applicable to skin cosmetics and harmless to the human body and are excellent in safety. The cosmetic composition shows excellent skin moisturizing effect, skin barrier improving effect and antioxidative effect. In addition, the cosmetic composition has excellent stability without causing discoloration, separation, and precipitation in response to temperature changes.

All technical terms used in the present invention, unless otherwise defined, are consistent with the meanings as commonly understood by one of ordinary skill in the art to which the present invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term " about " is used herein to refer to a reference amount, level, value, number, frequency, percentage, dimension, size, quantity, weight or length of 30, 25, 20, 15, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words " comprises " and " comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

The present inventors have studied the applicability of nematode and peony root extract as a cosmetic product among various natural products, and after various experiments, they have found that antioxidation, moisturizing and skin barrier improvement effects can be expected as a cosmetic product. Such excellent efficacy has resulted in a maximally high yield of nimabi and peony extracts, thereby developing a cosmetic composition showing even more excellent effects.

Azadirachta Indica , one of the natural materials of the present invention, is a tree that grows in India, with white flowers blooming in February and March, and fruits in July and August. The oil of the nymphs is used extensively, such as making pesticides, and the nymph is used as the primary processing material for pesticides and medicines. For example, oil extracted from seeds of nymphs is used for waxes and lubricants, and seeds for seeds are used as fertilizers. In addition, the nymph is widely used as an indigenous animal in India, and is also a useful tree for livestock feeding. It is also known as a panacea, and has been planted as a roadside tree in India. It has a germicidal effect and acts like a ginkgo tree. In addition, nimyeo is effective as medicine (skin disease, diabetes) is being studied by many scientific researchers.

Paeonia lactiflora , another active ingredient contained in the composition of the present invention, is a perennial dicotyledonous plant belonging to the family Ranunculaceae, and is distributed in Korea, Mongolia and East Siberia.

In the present invention, in order to exhibit the maximally high yield and optimum effect of nematodes and peanuts having various effects, when the nematode and peony are subjected to the ultrasonic treatment and the black yeast fermentation, the content of the effective ingredient is increased , And when it was applied to a cosmetic composition, it was found that it was excellent in moisturizing, skin barrier improvement and antioxidant effect, and thus the present invention was completed.

The extraction site of the nodular extract may be a plant, a branch, a seed, or a root, more preferably a nodular seed.

The extraction site of the peony extract may be a variety of organs (e.g., leaves, flowers, stems and roots) of peony, and preferably a roots of mature peony.

The effective ingredients of the cosmetic composition of the present invention, nimabi and peony, are first subjected to ultrasonic extraction. The ultrasonic extraction is carried out by dissolving dried nimabi seeds and peanut roots in water, anhydrous or lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, Ethyl ether, butylene glycol, hexane, and mixtures thereof, followed by extraction with an ultrasonic extractor for about 1 to 12 hours. The time of the ultrasonic extraction may be varied depending on the amount of the sample to be extracted, and the result of the extraction may be filtered or purified.

Among the above solvents, alcohols are preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, and most preferably 7 times the dry weight of the nymphs.

Further, it means extraction with about 1 to 12 hours by using an ultrasonic extractor while maintaining a pH of 5 to 7 at a temperature condition (room temperature) of preferably 20 to 40 占 폚.

The ultrasonic treatment of nimabi and peony and the black yeast fermentation extract of the present invention can be produced by fermenting the above-mentioned ultrasonic extract of nimabi and peonies with black yeast. Specifically, it can be produced by the following method. First, 1% to 5% by weight of glucose is added as a carbon source to the ultrasonic extract of nimabi and peony root, and 0.1% to 1%, preferably 0.2% to 0.5% by weight of peptone is further added to sterilize. To this, 25 g / L of black yeast ( Aureobasidium pullulans ) can be inoculated and cultured. The culture can be carried out using a 5 L fermenter for 1 to 7 days at 20 to 40 ° C while maintaining the pH at 5-7. After culturing, the culture broth was centrifuged to remove the culture medium first, and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the cultured ginseng and the peony fermented product are removed by refluxing by adding ethanol to make a final 70% (v / v) ethanol aqueous solution, followed by cold filtration and filtration. Upon completion of the filtration, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to produce nimabi extract and peony root extract fermented as black yeast after the ultrasonic extraction of the present invention.

In addition, the nimabi extract of the present invention may be produced in powder form by an additional process such as vacuum distillation, freeze drying, spray drying and the like.

According to a preferred embodiment of the present invention, the extract of nimabi and peony powder added to the cosmetic composition is 0.0001 to 30% by weight, preferably 0.01 to 20% by weight, and most preferably 0.1 to 20% by weight based on the total weight of the cosmetic composition. 10% by weight. When the content of the extracts of nimabi and peony is less than 0.0001% by weight, the effect of improving antioxidation, moisturizing and skin barrier is insignificant, and when it is more than 30% by weight, economical efficiency is low.

The ingredients contained in the cosmetic composition of the present invention include, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Production Examples. These production examples are only for explaining the present invention in more detail and that the scope of the present invention is not limited by these production examples according to the gist of the present invention, It will be obvious.

Manufacturing example  One : Nemo and  Preparation of solvent extract of peony

The nimabe seeds and peanut roots were selected, thoroughly washed and dried, and then mixed and pulverized at a ratio of 5: 5 (weight ratio) and finely ground using a sieve of 100 mesh. 70% ethanol was added thereto so as to have a weight of 100 g / L, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper, Concentrated under reduced pressure and lyophilized. The lyophilized product was dissolved in 50% glycerin to 2% (w / v) to prepare a solvent extract of nimabi and peony.

Manufacturing example  2 : Nemo and  Production of warmed extract of peony

The nimabi seeds and peanut roots were selected, thoroughly washed and dried, and then mixed and pulverized at a ratio of 5: 5 (weight ratio) and finely ground using a 300 mesh sieve. 70% ethanol was added thereto so as to have a weight ratio of 100 g / L, and the mixture was refluxed at 95 ° C for 4 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper, And concentrated under reduced pressure and lyophilized. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (w / v) to prepare warmed extracts of nematode and peony.

Manufacturing example  3: Nemo and  Manufacture of Ultrasonic Extract of Peony

Nimabi seeds and peanut roots were selected, thoroughly washed and dried, and then mixed and pulverized at a ratio of 5: 5 (weight ratio) and finely ground using a 300-mesh sieve. 70% ethanol was added to 100 g / L of the crushed walnut seeds and peanut roots, and the mixture was extracted with an ultrasonic wave extractor (natural solution) at room temperature for 4 hours. After cooling, it was filtered with a filter of whatman # 3 , Concentrated under reduced pressure at 50 DEG C or below and freeze-dried. The lyophilized product was dissolved in 50% glycerin to 2% (w / v) to prepare an ultrasonic extract of nematode and peony.

Manufacturing example  4 : Nemo and  Ultrasound treatment of peony Black yeast  Preparation of fermented extract

The ultrasonic treatment of nematode and peony and the black yeast fermentation extract were prepared by fermenting an ultrasonic extract of nymphenicol and peony root of Preparation Example 3 with black yeast. Specifically, 2% by weight of glucose was added as a carbon source to the ultrasonic extract of nimabi and peony root of Preparation Example 3, and 0.5% by weight of peptone was further added to sterilize. Then, the culture was inoculated with a culture solution of Aureobasidium pullulans strain so as to have a concentration of 25 g / L. Cultures were cultured in a 5 L fermenter for 7 days at 37 ° C and pH 6. After culturing, the culture broth was centrifuged, and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented product of the nymph and the peony which had been firstly removed from the culture was refluxed with ethanol to make a final 70% (v / v) ethanol aqueous solution, cooled and then filtered. Upon completion of the filtration, the extract obtained in the extraction step was transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to produce an ultrasonic treatment of nimabi and peony root and a black yeast fermentation extract.

Manufacturing example  5: Dear  Ultrasonic processing and Black yeast  Preparation of fermented extract

The ultrasound treatment and the black yeast fermentation extract of nimabi were prepared by preparing nimabi (son) ultrasound extract by using the method of Preparation Example 3 and fermenting it with black yeast. Specifically, 2% by weight of glucose was added as a carbon source to the edible nymph extract prepared using the method of Production Example 3, and 0.5% by weight of peptone was further added to sterilize the extract. To this, 25 g / L of black yeast ( Aureobasidium pullulans ) were inoculated and cultured. Cultures were cultured in a 5 L fermenter for 7 days at 37 ° C and pH 6. After culturing, the culture broth was centrifuged, and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented broth obtained by removing the cultures first was added with ethanol to make a final 70% (v / v) ethanol aqueous solution, refluxed, cooled, and filtered. Upon completion of the filtration, the extract obtained in the extraction step was transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to prepare an ultrasonic treatment of nematode and a black yeast fermentation extract.

Manufacturing example  6: Ultrasonic treatment of peony and Black yeast  Preparation of fermented extract

Peony extracts fermented with black yeast after ultrasonication were prepared by preparing an extract of peony root (Root) using the method of Preparation Example 3 and fermenting it with black yeast. Specifically, 2% by weight of glucose was added as a carbon source to the sonic extract of P. monocytogenes prepared using the method of Preparation Example 3, and 0.5% by weight of peptone was further added to sterilize. To this, 25 g / L of black yeast ( Aureobasidium pullulans ) were inoculated and cultured. Cultures were cultured in a 5 L fermenter for 7 days at 37 ° C and pH 6. After culturing, the culture broth was centrifuged, and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the cultured fermented product was firstly removed from the fermented product by adding ethanol to the final 70% (v / v) ethanol solution, refluxing, cooling and filtering. Upon completion of the filtration, the extract obtained in the extracting step was transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to prepare an ultrasonic treatment of monoculture and a black yeast fermentation extract.

Prescription example  One

Example 1 Preparation Example 1 of the cream containing the ultrasonic treatment of nimabi and peony and the fermented black extract of Preparation Example 4 is shown in Table 1 below.

ingredient Content (unit:% by weight) Ultrasonic treatment of nodule and peony and black yeast fermentation extract (Preparation Example 4) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Comparative Prescription Example  1>

Comparative Example 1 of the cream in cosmetics containing the ultrasonic extract of nymphal and peony root of Preparation Example 3 is shown in Table 2 below.

ingredient Content (unit:% by weight) (Preparation Example 3) &lt; RTI ID = 0.0 &gt; 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Prescription example  2>

Table 2 shows the comparative formulation example 2 of the cream containing no nematode and peony extract.

ingredient Content (unit:% by weight) glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

compare Prescription example  3

Comparative Example 3 is shown in the following Table 4 as an example of the formulation of the cream in cosmetics containing the solvent extract of nimabi and peony root of Preparation Example 1.

ingredient Content (unit:% by weight) Solvent Extract of Nodame and Peony (Preparation Example 1) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

compare Prescription example  4

The comparative formulation example 4 of the cream containing the warm-boiled extract of nimabi and peony in Preparation Example 2 is shown in Table 5 below.

ingredient Content (unit:% by weight) (Example 2) &lt; tb &gt; &lt; tb &gt; 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

Comparative Prescription Example  5

Example 5 The formulation 5 of the cream containing the ultrasonic treatment of nymphea and the black yeast fermented extract of Preparation Example 5 is shown in Table 6 below.

ingredient Content (unit:% by weight) Ultrasonic treatment of nematode and black yeast fermentation extract (Preparation Example 5) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

Comparative Prescription Example  6

Table 6 shows the prescription example 6 of the cream containing the ultrasonic treatment of Peony root of Preparation Example 6 and fermented black yeast extract.

ingredient Content (unit:% by weight) Ultrasonic treatment of peony and black yeast fermented extract (Preparation Example 6) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

Experimental Example  1: Antioxidant effect measurement

Antioxidant activity of each of the extracts prepared in Production Examples 1 to 6 of the present invention was measured by the Tiobarbituric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction. That is, in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 μl of ethyl linoleate, 0.2% sodium dodecylsulfoxide (SDS), 10 μmol of ferrous chloride and 2 μmol of hydrogen peroxide, The dimethylsulfoxide (DMSO) test solution of one extract was added and incubated at 55 ° C for 16 hours. Then, 50 μl of 4% dibutylhydroxyltoluene (BHT) was added to stop the reaction. 0.3 ml of this solution was put into a test tube, and 3.0 ml of 0.05 N hydrochloric acid and 0.67% thiobarbituric acid (TBA) solution were added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer after centrifugation was measured at 535 nm. The antioxidative effect of each extract was calculated by the following equation (1). The results are shown in Table 8 below.

Test substance Antioxidative effect(%) Experimental concentration (10 / / ml) Experimental concentration (50 / / ml) Experimental concentration (100 / / ml) Production Example 1 21 45 60 Production Example 2 28 50 62 Production Example 3 30 55 76 Production Example 4 40 68 90 Production Example 5 31 56 77 Production Example 6 30 55 78

The antioxidative effects of the nimaburi extracts and the peony extracts of Preparation Examples 1 to 6 were mostly high, but the antioxidative effects of the nimabi and peony extract and the fermented black yeast extracts of Preparation Example 4 according to the present invention were very high It can be seen that it is excellent.

The nimaburi extracts and peony extracts of Preparation Examples 1 to 6 were mixed with ascorbic acid, dl-alpha-tocopherol, beta-carotene and dibutylhydroxyltoluene BHT) was measured by the same method as Experimental Example 1 to compare the IC ₅₀ of 50% inhibitory concentration. The results are shown in Table 9 below

Test substance IC ₅₀ (10 μg / ml) Production Example 1 50.5 Production Example 2 47.4 Production Example 3 41.2 Production Example 4 30.5 Production Example 5 40.5 Production Example 6 41.7 Ascorbic acid 21.90 Dl-alpha-tocopherol &lt; / RTI &gt; 3.36 Beta-carotene 29.8 Dibutylhydroxyltoluene (BHT) <1

The nimabue extracts and peony extracts of Preparation Examples 1 to 6 showed high activities comparable to those of the other antioxidants, but the extracts of the nymphenicol and peony root of Example 4 and the black yeast fermentation extract showed high antioxidative activity Able to know. Especially, it was found that the nimda extracts and peony extracts showed significant antioxidant activity despite the natural product mixture. Especially, the antioxidative activity effect of nimoo and peony extract and black yeast fermented extract (5: 5 mixture) The most improvement was found.

Experimental Example  2 : Nemo and  Ultrasound treatment of peony Black yeast  Evaluation of skin moisturizing effect of fermented extract

The wrinkle-improving effect of the cream (Formulation Example 1) and the creams of Comparative Formulation Examples 1 to 6 containing the ultrasonic treatment of nematocritis and peony root and fermented black yeast extract was evaluated as follows.

Twenty-five to twenty-four to twenty patients without skin disease were divided into two groups of 20 persons per one group. The creams of the prescription examples 1 and the comparative prescription examples 1 to 6 were administered twice daily for 1 month to face and I had to apply it to Park. The skin conductivity was measured by using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The rate of increase in skin conductivity (%) was measured and the rate of increase was evaluated. The results are shown in Table 10 below.

Experimental product After 1 week after 2 weeks After 3 weeks Prescription Example 1 55 60 69 Comparative Prescription Example 1 43 48 52 Comparative Prescription Example 2 17 20 24 Comparative Prescription Example 3 19 25 31 Comparative Prescription Example 4 25 28 35 Comparative Prescription Example 5 20 25 30 Comparative Prescription Example 6 19 24 29

As shown in Table 10, in case of Prescription Example 1 containing ultrasound treatment of nymphea and peony and fermented black yeast extract, the skin conductivity increase rate was much higher than Comparative Prescription Examples 1 to 6. In general, the skin conductivity is proportional to the skin moisture content, and thus the above results confirm that the cosmetic containing the nymphenic and peanut sonication and the black yeast fermented extract keeps the skin moisture content higher than that of the non-cosmetic cosmetic. Skin irritation could not be observed in most of the subjects who applied this cosmetic composition to the skin.

Experimental Example  3: Nemo and  Ultrasound treatment of peony Black yeast  Evaluation of skin barrier improvement effect of fermented extract

In order to examine the skin barrier improvement effect of the cosmetic comprising the ultrasonic treatment of nimabi and peony root and fermented black extract of the present invention, the following experiment was conducted. In this Experimental Example, the cosmetic material of Formulation Example 1 was evaluated by performing a comparative experiment using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) for transepidermal water loss (TEWL). Each sample was applied to the right and left faces of 40 women in their 20s and 40s. Percutaneous water loss before, 1, 2, 4, and 6 hours was applied before and 3 cm from the eye. The results are shown in Table 1. The transdermal water loss was measured using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) three times and the mean value was evaluated. The results are shown in Table 11 below.

division 1 hours 2 hours 4 hours 6 hours Prescription Example 1 9.2 8.5 7.8 6.8 Comparative Prescription Example 1 15.3 14.9 12.1 8.9 Comparative Prescription Example 2 22.0 21.5 20 16 Comparative Prescription Example 3 18.9 17.3 15.2 13.1 Comparative Prescription Example 4 17.0 16.2 14.2 12.1 Comparative Prescription Example 5 17.5 15.4 14.6 13.7 Comparative Prescription Example 6 16.8 15.9 14.1 12.5

As shown in Table 11, the amount of transdermal water loss was measured. As a result, the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied ultrasonic treatment with nimabi and peony extract and cream containing black yeast fermented extract, It was found that there was a better effect on improvement.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the invention is defined by the appended claims and their equivalents.

Claims (6)

A cosmetic composition for skin moisturizing, comprising an ultrasonic treatment of a mixture of nimabi seeds and peanut roots at a weight ratio of 1: 1 and an extract of fermented black yeast. The cosmetic composition for skin moisturizing according to claim 1, wherein the cosmetic composition has a skin barrier improving effect. The method according to claim 1, wherein the mixture of the nimabi seeds and the peanut roots is subjected to ultrasonic treatment and black yeast fermentation extract. The mixture of nimabi seeds and peanut root is mixed with purified water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate , Butyl acetate and an extraction solvent selected from the group consisting of 1,3-butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane and mixtures thereof, And then fermented with black yeast. [Claim 2] The method according to claim 1, wherein the ultrasonic treatment and the black yeast fermentation extract of the mixture of nimabi seeds and peanut roots are carried out by using an ultrasonic extract of nimabi and peony root as black fungi at a temperature of 20 to 40 DEG C while maintaining a pH of 5 to 7 , And fermented for 1 to 7 days. The cosmetic composition for skin moisturizing according to claim 1, wherein the mixture of the nimabi seeds and the peanut root is sonicated and the black yeast fermented extract is contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the cosmetic composition is at least one selected from the group consisting of: