KR101862006B1 - Sex pheromone composition for attracting eurytoma maslovskii and method for preparing thereof - Google Patents

Abstract

The present invention relates to a pheromone composition for lubrication of peach seeds and a method for producing the pheromone composition, and more particularly, to a pheromone composition containing (E) -2-hexenyl butyrate, methyl salicylate and tetradecane Through this, pheromone trap, which is an outbreak tool, and mite disruption agent, which is an environmentally friendly insect pest control agent, can be produced. Thus, it is possible to effectively prevent occurrence of plums caused by peach seedling.

Description

TECHNICAL FIELD [0001] The present invention relates to a pheromone composition and a method for producing the same. [0002] The present invention relates to a pheromone composition,

The present invention relates to a pheromone composition for peach fungi, and a process for producing the same.

Eurytoma maslovskii ( Nikol'skaya ), which occurs in the southern part of the province in the southern part of the country, has been causing great damage since the larvae eat the plum seeds (average rate of damage from 2013 to 2015: 33 ~ 67%).

In particular, peach seedlings causing damage to plums were identified in plums, apricots, and Chinese peach seeds in Gyeonggi Province, Chungcheongnambuk Province, Jeolla Province and North Jeolla Province, and peach seedlings were found in the nuclei of nuclear plants, . The pupa was observed from the end of March to the end of April, and the larvae were more than 90% alienated from late April to early May. Females scattered beneath the outer shell of the fruit seeds when the seeds were liquid before the nucleus was cured. The length of the spawning ducts did not exceed 5 mm, and the size of the plumable plums did not exceed 2 cm in diameter. A maximum of 5 eggs were observed in one fruit, and the egg duration was not more than 2 days. The hatching larvae first migrate to the solidifying ship and feed on it. During this process, only one larva survives due to the competition between the larvae. In June, most of the injured fruits suffered from corruption.

In case of excessive use of insecticide for controlling the insecticide resistance, the ecosystem destruction due to reduction of natural enemies and useful insects, lethal toxicity due to residual toxicity, And the like.

Sex pheromone has been applied to advanced countries such as USA and Europe as a way to solve this problem. Sex pheromone is a kind of pheromone which is a communication material in the same species of living things. It is a communication substance that diverges from one side and attracts relativity to mate. Organic synthetic insecticides have the advantage of being able to maintain the effect by continuously treating the effect of the pheromone in a very small amount, for example, 10 to 20 g per 2 or 3 times, unlike in the case of spraying a large amount to nonspecifically eradicate insects . That is, sex pheromones act species-specific, are not toxic, and do not affect other organisms. The area where sexual pheromone is used is in the field of prevention by the occurrence of the occurrence, mass suckling and mating disturbance. In the field of incidence, the occurrence situation of the insect pest is continuously examined to judge whether the insecticide treatment is necessary, And it is the basis of integrated pest management that can prevent the abuse of pesticides and preserve the ecosystem

However, until now, it has been difficult to detect the occurrence of sex pheromone in peach seeds and to detect the occurrence of pests and to control the environment. Therefore, there is a need for a sex pheromone composition most suitable for attracting peach seedlings, a mating disruptor composition using the same, and a method for producing the same.

The object of the present invention is to provide a process for the preparation of (E) -2-hexenyl butyrate, methyl salicylate and tetradecane as an active ingredient by means of GC-MS analysis on Eurythmia maslovskii female ferrocommon extracts Wherein the pheromone composition comprises a pheromone composition.

It is another object of the present invention peach seed years jombeol (Eurytoma maslovskii) GC-MS analysis result of day-to-day furnace, (E) -2- hexenyl butyrate, methyl salicylate and the effective tetradecane seen in female sex Perot door extracting Mel The present invention provides a method for producing a pheromone composition of peach seeds which contains as an ingredient an extractable pheromone composition.

It is another object of the present invention peach seed years jombeol (Eurytoma maslovskii) GC-MS analysis result of day-to-day furnace, (E) -2- hexenyl butyrate, methyl salicylate and the effective tetradecane seen in female sex Perot door extracting Mel Which is contained in the composition of the present invention.

It is another object of the present invention peach seed years jombeol (Eurytoma maslovskii) GC-MS analysis result of day-to-day furnace, (E) -2- hexenyl butyrate, methyl salicylate and the effective tetradecane seen in female sex Perot door extracting Mel As a component, a method for preparing a mating disruptor composition for controlling peach seedlings.

However, the problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned can be clearly understood by those skilled in the art from the following description.

The pheromone composition of the present invention for peach fungicidal use contains noonnar, (E) -2-hexenyl butyrate, methyl salicylate and tetradecanol as active ingredients.

The pheromone composition according to the present invention for peach fuels is prepared by mixing nanolal, (E) -2-hexenyl butyrate, methyl salicylate and tetradecane.

The composition of the present invention for preventing peach seedling mothballs contains (N) - (E) -2-hexenyl butyrate, methyl salicylate and tetradecanol as active ingredients.

The present invention relates to a mating disruptor composition for controlling peach seedlings, which is prepared by mixing nanolal, (E) -2-hexenylbutyrate, methyl salicylate and tetradecane.

The above-described task solution is merely exemplary and should not be construed as limiting the present invention. In addition to the exemplary implementations described above, there may be additional implementations and embodiments described in the drawings and detailed description of the invention.

Through the composition according to the present invention, pheromone trap as an outbreak tool and eco-friendly insect control agent, mating disruptor, can be produced and effective damage prediction and eco-friendly control can be performed, thereby reducing the damage of plums caused by peach seedlings.

Fig. 1 is a graph showing the results of GC-MS analysis of the extracts of the chest areas of the peach seedlings.
FIG. 2 is a photograph showing traps for pheromone inducing effect used in the embodiment of the present invention and adult male peach seedlings.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. It should be understood, however, that the present invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly explain the present invention in the drawings, portions not related to the description are omitted.

Throughout this specification, when an element is referred to as "including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

The terms "about "," substantially ", etc. used to the extent that they are used throughout the specification are intended to be taken to mean the approximation of the manufacturing and material tolerances inherent in the stated sense, Accurate or absolute numbers are used to help prevent unauthorized exploitation by unauthorized intruders of the referenced disclosure. The word " step (or step) "or" step "used to the extent that it is used throughout the specification does not mean" step for.

Throughout this specification, the term "combination thereof" included in the expression of the machine form means one or more combinations or combinations selected from the group consisting of the constituents described in the expression of the machine form, And the like.

Throughout this specification, the description of "A and / or B" means "A, B, or A and B".

The pheromone composition of the present invention for peach fungicidal use contains noonnar, (E) -2-hexenyl butyrate, methyl salicylate and tetradecanol as active ingredients.

The inventors of the present invention have studied the chemical components of the peach fleshy beetle, which has a peach fleshy mature male females, by scissors cutting the contents in a hexane and analyzed by GC-MS. As a result, (E) -2-hexenyl butyrate, methyl salicylate, and tetradecanal) are present at a constant weight ratio in the range of Respectively. As a result of investigating the attracting effect of the peach seedlings on the males in the plum orchard by various compositional compositions, it was found that the mixture of the four components of nanolal, (E) -2hexenylbutyrate, methyl salicylate and tetradecanoal The peach seeds were found to be attracted to the male, and the present invention was completed.

The pheromone composition according to the present invention is characterized in that the pharmacologically acceptable pheromone composition is prepared by mixing the nonanal, (E) -2-hexenylbutyrate, methyl salicylate and tetradecaneal in a weight ratio of 50: 30 to 300: 800: May be contained in a weight ratio of 50:50 to 200: 800: 50, more preferably 50:80 to 120: 800: 50. Especially, the (E) -2-hexenylbutyrate is the component having the greatest influence depending on the variation among the four components, and the methyl salicylate is the component having the largest amount, and the (E) -2-hexenylbutyrate and the methyl salicylate The weight ratio of the silicate may be 50 to 200: 800, preferably 80 to 120: 800. If the weight ratio is exceeded, the effect of inducing peach seedlings may be significantly reduced.

(E) -2-hexenyl butyrate, methyl salicylate, and tetradecane, which are included in the peach fungicidal lyophilate composition of the present invention, are not limited to the peach fungus ( Eurytoma maslovskii ) Lt; RTI ID = 0.0 > pheromone < / RTI >

According to another aspect of the present invention, there is provided a method for preparing a peach seedling attractant pheromone composition comprising mixing sunflower, (E) -2-hexenyl butyrate, methyl salicylate and tetradecane / RTI >

(E) -2-hexenyl butyrate, methyl salicylate and tetradecane are used in a weight ratio of 50: 30 to 300: 800: 50, preferably 50:50 To 200: 800: 50, and more preferably 50: 80 to 120: 800: 50. In particular, the weight ratio of the (E) -2-hexenyl butyrate and the methyl salicylate may be 50 to 200: 800, preferably 80 to 120: 800. If the weight ratio is exceeded, the effect of inducing peach seedlings may be significantly reduced.

The method for preparing a sex pheromone composition of the present invention is characterized in that, before mixing the above components, the pharyngeal extract of Eurytoma maslovskii is mixed with the nonanal, (E) -2-hexenyl butyrate, methyl salicylate and tetra And separating the decane al.

According to another aspect of the present invention, there is provided a mating disruptor composition for controlling peach seedlings comprising a mixture of sunflower, (E) -2-hexenylbutyrate, methyl salicylate and tetradecane as an active ingredient / RTI > According to still another aspect of the present invention, there is provided a mating disruptor composition for controlling peach seedlings, comprising a step of mixing sunflower, (E) -2-hexenylbutyrate, methyl salicylate and tetradecane Is provided.

The composition of the mating disruptor composition for controlling peach seedling of the present invention and the method for its production can be applied to the same composition of the above-described peach fungicidal losarticidal pheromone composition and its production method, A person skilled in the art can appropriately modify and apply the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

[Example]

1. Sex pheromone component of peach females

The chemical components of Eurythoma maslovskii were cut with scissors to extract the contents in hexane and analyzed by GC-MS. The results showed that the four components {nonanal, (E (E) -2-hexenyl butyrate, methyl salicylate, and tetradecanal} were found in a weight ratio of 5: 10: 100: 5.

GC-MS analysis of the sex pheromone extract of Pinus densiflora showed the same results as in Fig.

2. Male infertility of peach seedlings as a pheromone component

Based on the results shown in FIG. 1, a composition having the composition ratios as shown in Table 1 below was prepared, and an experiment was conducted in which the male peach seedlings were attracted by using the trap for pheromone attracting effect test shown in FIG. 2 outdoors. Table 1 shows the number of attracted males per trap.

nonanal
(㎍) ( E ) -2-hexenyl
butyrate
(㎍) methyl
salicylate
(㎍) tetradecanal
(㎍) The
(Horse / trap) Example 1 50 200 800 50 10.7 Example 2 50 100 800 50 28.3 Example 3 50 50 800 50 3.0 Comparative Example 1 50 100 800 0 0 Comparative Example 2 50 100 0 50 0 Comparative Example 3 50 0 800 50 0 Comparative Example 4 0 100 800 50 0 Comparative Example 5 0 0 0 0 0

As can be seen from the above Table 1, all of the above four components are required for the peach flesh to act as a sex pheromone attracting male birds. Especially, the content ratio of (E) -2-hexenyl butyrate It was confirmed that it acted importantly.

3. Peach seeds of pheromone trap prepared by using the sex pheromone composition of the present invention as a bait,

In Table 1, pheromone traps with the sex pheromone composition of Example 2, which had the highest number of recipients, were set up to conduct mass trapping control experiments of the peach seedlings. The results are shown in Table 2 below.

   division Breathing water (marine / trap) Fruit damage rate (%)    Mass capture control 340.3 2.3    No protection (trap uninstalled) - 17.7

* Contents of four components per pheromone bait: 1,000 g (50: 100: 800: 50)

* Number of pheromone trap installed for mass capture: 50 / ha

As can be seen from the above Table 2, it was confirmed that when the sex pheromone composition of the present invention was installed, it was possible to capture a large amount of peach seedlings, and the fruit loss ratio was remarkably reduced as compared with the case of no seedlings.

4. Peach seedlings with mating disruptor composition of the present invention

In Table 1, the composition of Example 2, which had the highest number of recipients, was placed on a peach tree with a polyethylene tube or the like, and the results are shown in Table 3 below.

   division Breathing water (marine / trap) Fruit damage rate (%)    Control of mating disturbance 2.3 1.7    No protection 128.7 19.3

* Pheromone disruption agent 4 ingredients content: 50mg (2.5: 5: 40: 2.5)

* Pheromone treatment concentration for mating disturbance: 50 g / ha

As can be seen from the above Table 3, when the mite-disrupter composition of the present invention is installed, the normal mating behavior of females and males of the peach seedling, which occurs naturally in the orchard, is disturbed or delayed, The damage of peach trees was confirmed to decrease.

It will be understood by those of ordinary skill in the art that the foregoing description of the embodiments is for illustrative purposes and that those skilled in the art can easily modify the invention without departing from the spirit or essential characteristics thereof. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed in a similar fashion may also be implemented.

The scope of the present invention is defined by the appended claims rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be interpreted as being included in the scope of the present invention.

Claims (10)

(E) -2-hexenyl butyrate, methyl salicylate, and tetradecane as an active ingredient.
The method according to claim 1,
The pheromone composition according to claim 1, wherein the weight ratio of the nonanal, (E) -2-hexenyl butyrate, methyl salicylate and tetradecane is from 50:30 to 300:800:50.
3. The method of claim 2,
Wherein the weight ratio of (E) -2-hexenyl butyrate and methyl salicylate is 100 to 200: 800.
The method according to claim 1,
The pheromone composition according to claim 1, wherein the nonanal , (E) -2-hexenyl butyrate, methyl salicylate and tetradecane are isolated from the female pheromone extract of Eurytoma maslovskii .
(E) -2-hexenyl butyrate, methyl salicylate, and tetradecane alcohols in the presence of an effective amount of a phthalic anhydride.
6. The method of claim 5,
Wherein the weight ratio of the nonanal, (E) -2-hexenyl butyrate, methyl salicylate and tetradecane is from 50:30 to 300: 800: 50.
6. The method of claim 5,
Wherein the weight ratio of (E) -2-hexenyl butyrate and methyl salicylate is 100 to 200: 800.
6. The method of claim 5,
Before the mixing step,
(E) -2-hexenyl butyrate, methyl salicylate and tetradecanol in the female pheromone extract of Eurytoma maslovskii ,
Wherein the mixing step comprises admixing the separated vanadium, (E) -2-hexenyl butyrate, methyl salicylate, and tetradecane aliquots.
The present invention relates to a mating disruptor composition for controlling peach seedlings, which comprises a mixture of sunflower, (E) -2-hexenyl butyrate, methyl salicylate and tetradecane as an active ingredient.
(E) -2-hexenylbutyrate, methyl salicylate, and tetradecane-alumina, in the presence of an effective amount of a compound of formula (I).

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