KR101810229B1 - Cosmetic composition for improving acne containing teatree leaves spicule powder - Google Patents

Abstract

One embodiment of the present invention is a combined extract comprising tea tree leaf extract, rice bran extract, rhododendron extract, and aloe vera leaf extract; And a fine needle powder as an active ingredient.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving acne,

The present invention relates to a cosmetic composition for improving acne containing fine needle powder, and more particularly, to a cosmetic composition for improving acne which has excellent anti-inflammation and antibacterial effect, comprising a natural extract and a fine needle powder as an active ingredient will be.

Acne is an inflammatory disease that occurs in the skin of hair follicles. 70-80% of the patients are concentrated in the age group of 11-25 years, and they were previously thought to be specific diseases for adolescent adolescents. However, these days, It is one.

Acne can develop symptoms due to an increase in sebum secretion, hyperkeratosis-induced follicular stenosis, proliferation of acne bacterium, inflammatory reaction, etc., and various mechanisms may be combined and adversely affected .

Although sebaceous hyperplasia, hair follicle keratinization, and acne bacterium proliferation are the main causes of acne, the hypertrophy of sebaceous glands, hyperkeratosis of hair follicles, and bacterial effects interact to aggravate acne and cause individual factors Acne can also be caused by.

In addition, genetic factors such as acne-prone constitution, external factors such as season and climate, food effects, fatigue and stress are also related to acne. The cause of acne has not yet been clarified, but it is reported that acne can be directly induced by the above-mentioned main causes, if genetic factors and environmental factors are excluded.

Acne can be treated through excessive suppression of sebum secretion and relief of inflammation. Therefore, the occurrence of acne can be effectively suppressed by eliminating a complex cause that causes acne.

In general, medicines in the form of oral or topical agents may be used to remove the major cause of acne and treat acne. Specifically, the present invention relates to a method for inhibiting sebum production using antihistamines, an antimicrobial agent such as resorcinol, sulfur, salicylic acid, benzoyl peroxide, retinoic acid, and isotretinoin for antiinflammatory action of steroid hormones and nonsteroidal antiinflammatory agents, A method of inhibiting the activity of propionibacterium acnes by an antibiotic substance such as a keratolytic agent, tetracycline, erythromycin, and macrocycline.

However, despite the fact that the acne treatment method has shown some success in treating acne, it shows some problems in terms of effectiveness, side effects, and usability.

Specifically, hormones induce hormone abuse side effects such as inhibition of epidermal growth. Keratolytic agents such as retinoic acid and benzoyl peroxide can cause contact dermatitis due to skin irritation. Antibiotics such as tetracycline may cause resistant bacteria And may be a factor of photosensitizing action.

Meanwhile, in the cosmetics industry, many products using natural products have been developed to reduce skin irritation caused by various chemical substances. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased. However, the cosmetic product containing the natural product extract obtained by a conventional method is not practically effective because antibacterial and anti-inflammatory functions are not effectively achieved and the activity of the extract is not maintained and controlled continuously.

Therefore, in order to treat and improve acne, it is necessary to develop natural cosmetic products which are not only safe to human body but also have excellent skin improving effect.

However, when a single natural raw material is used, various kinds of effective ingredients for realizing the skin improvement effect may not be included, and thus it is suitable for the development of multi-functional cosmetics, which is gradually becoming a trend in the cosmetics industry Therefore, various studies have been made to improve this.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above problems, and an object of the present invention is to provide an anti- And to provide a cosmetic composition for improvement.

In order to achieve the above object, one aspect of the present invention is a combined extract comprising tea tree leaf extract, rice bran extract, rhododendron extract, and aloe vera leaf extract; And a fine needle powder as an active ingredient.

In one embodiment, the micropowder powder may be obtained from a sponge animal.

In one embodiment, the sponge animal may be a Spongilla lacustris .

In one embodiment, the average length of the fine needle-like powder particles may be 60 to 500 mu m.

In one embodiment, the micropowder powder may be from 0.5 to 10.0% by weight based on the total weight of the composition.

In one embodiment, the combined extract may comprise from 20 to 40 parts by weight of tea tree leaf extract, from 10 to 20 parts by weight of rice bran extract, from 10 to 20 parts by weight of an obovate extract, and from 10 to 20 parts by weight of an aloe vera leaf extract .

In one embodiment, it may be for acne improvement or skin cleansing.

In one embodiment, at least one selected from the group consisting of softening longevity, nutritional lotion, nutritional cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder .

According to the present invention, the cosmetic composition of the present invention contains a complex extract derived from tea tree leaves and other natural plants as an active ingredient, thereby improving the skin improving effect, particularly the anti-inflammation and antibacterial effect.

In addition, by including the fine needle powder, a synergistic effect with the combined extract can be obtained, thereby maximizing the skin improvement effect of the cosmetic composition. Particularly, the fine needle powder penetrates into the pores to kill acne bacteria, thereby suppressing skin troubles and improving the skin soothing effect.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a combination extract comprising tea tree leaf extract, rice bran extract, rhizome extract, and aloe vera leaf extract; And a fine needle powder as an active ingredient.

Since the cosmetic composition contains the above-mentioned complex extract as an active ingredient, it has excellent antimicrobial and anti-inflammatory activity and is highly effective in improving acne. In addition, it suppresses the activity of acne bacterium, so that the skin soothing effect is excellent. In particular, the cosmetic composition of the present invention has a synergistic effect of each component contained in the complex extract, so that the skin improvement effect can be remarkably enhanced compared with a cosmetic composition containing the single extract.

The " extract " refers to a solvent in which an effective ingredient contained in an extraction material is transferred by bringing a solvent and an extraction material into contact under specific conditions. If the substance is obtained by extracting a component contained in a raw material from a natural product, Regardless of the type. For example, it may include a component obtained by extracting a component dissolved in a solvent from a natural product using water or an organic solvent, a specific component of a natural product such as a product obtained by extracting only a specific component such as oil, and the like.

The " Melaleuca " alternifolia "belongs to the genus Leptospermum, Melaleuca, Kunzea, and about 86 species of Leptospermum genus and about 230 species of Melaleuca are known worldwide. The country of origin is native to New South Wales, Australia and grows in swamps up to about 6 meters in height. It is a kind of herb that plays a role of refreshing air, and it is a strong fragrance that gives a cool, clean and clean impression. It has a thin, light yellow liquid. The tea tree leaf extract is prepared by extracting tea tree leaves by steam distillation.

The tea tree contains terpinen-4-ol, g-terpinene, 1,8-cineol, etc., and is effective for astringent and acne skin by its convergence, cleanliness and purification effect. Especially, And is effective in improving and preventing acne by sterilizing effect.

In addition, Australian Aboriginal peoples have long since been treating infections caused by cuts of leaf tea tree leaves. During the Second World War, it was paid to soldiers in the tropics as a treatment for skin wounds. It is used in surgery and dentistry. It is also used in disinfectant, deodorant, soap and air purifier. It is also effective for various infectious diseases, cold, bad breath, athlete's foot, dandruff. However, since the irritation is strong, it is preferable to dilute the stock solution. Because of this strong irritation, the tea tree extract can be diluted to 2% in carrier oils such as almonds, apricots and hazelnuts, and is the most powerful immunostimulant with antibacterial, antiviral and antifungal effects, It is possible to construct a defense line against the organism invading into the body and to shorten the period of the disease.

The above-mentioned "rice bran" refers to a crumbling mixture such as peel, seed coat, and a paste layer which is formed when a brown rice is sown and made into a pure white rice, and in Chinese characters It is called rice bran. Generally, rice bran of 6 ~ 8% of brown rice is produced. If the degree of brown rice is increased, the amount of rice bran is increased. When the amount is high, fat, vitamin B1 and phosphorus are increased and the fiber is decreased. The standard chemical composition of rice bran is moisture content 13.5%, protein 13.2%, fat 18.3%, carbohydrate 38.3%, fiber 7.8%, ash 8.9%, vitamin B1 contains 2.5mg of 100g,

The " Rhus Semialata "is a wormhole formed by the parasitic bugs on leaves of Rhus verniciflora (Rhus verniciflua) with rhododendron larvae, and the appearance is uneven, irregularly split into 2 ~ 4 pockets or broken. Outer side is grayish grayish brown, covered with light brown short hair, hard and brittle. The genus is empty, but grayish white color or dead worms and secretions may remain. It is used to treat chronic seawater, old cough, old diarrhea, diarrhea, cold sweat, oil wells, which are caused by the strong convergence of the lungs, and to treat fecal bleeding, nosebleeds, uterine bleeding, trauma bleeding, early dermatitis and itching Has come. The pharmacological effect is effective for the convergence effect, the antibacterial action, the hepatic function-protecting action, and the antioxidative action.

The " Aloevera " is native to the warm, dry climate region of Africa and was used by ancient Egyptians to treat skin wounds, burns and inflammation. Since the early 1930s, it has been used in hospitals for treatment of mild skin diseases and radiation dermatitis. The "aloe" of the aloe vera is a substance that is tasted in Arabic, and "vera" means the truth in Latin. It is a perennial herbaceous stem with very short stem, leaf gathered at the end of the stem and juicy, juice is called presbyopia, and it contains aloein, coumaric acid, protein, and calcium.

Aloe vera is also known to be effective in the treatment of skin wounds and lacerations, has anticancer activity, and is also effective in allergic rhinitis and fever. The aloe vera is a raw material ingredient that continuously maintains the position of top 10 in various food and cosmetic formulations for the past 20 years. Recently, the use of aloe vera has been increased, and the number of end products containing aloe is more than 1500 .

In one embodiment, the combined extract may comprise 20-40 parts by weight of a tea tree leaf extract, 10-20 parts by weight of a rice bran extract, 10-20 parts by weight of an obovate extract, and 10-20 parts by weight of an aloe vera leaf extract .

When the variation in the mixing ratio of the complex extract is large, the skin improvement effect derived from the active ingredient of each extract may not be suitably implemented, so that the mixing ratio can be appropriately controlled in consideration of the working environment and the quality of the final product . The tea tree leaf extract, rice bran extract, rhododendron extract, and aloe vera leaf extract can be mixed at a weight ratio of 2: 1: 1: 1, and an optimal skin improvement effect can be realized within the above range.

The above-mentioned " Spicule " is a composite material composed of calcium and silicate in the form of various fine spicules collected from sponges inhabiting freshwater in Europe and Russia, and is generally obtained in the form of powder can do.

In one embodiment, the micropowder powder can be obtained from a spongy animal, wherein the spongy animal is a Spongilla lacustris L. ), Spongilla fragile Leidy , and Ephydatia fluviatilis species, but are not limited thereto.

The sponge animal is a primitive marine creature with no muscle, nerves or organs, and more than 5,000 species are present. The skeleton is a tissue that functions as a needle-like skeleton existing in the body of invertebrate animals, and mainly contains silicic acid and calcium carbonate. The sponge animal may comprise uniaxial, triaxial, tetragonal or multiaxial bone fragments containing calcium carbonate or silicic acid as a major component.

The sponge is sponge of various colors such as orange or cyan, and it lives mainly in the sea, but there is also a freshwater sponge.

The sponge animal is an organism that is in the initial stage of tissue in a state where cells are aggregated, and the body is composed of sponge fibers, spicules forming body wall, and cells bound to bone fragments. The main component of the bone fragments is calcite, silicic acid, silica or the like, and the size is about 100 탆 or so and can be observed by a microscope.

The bone fragments can be used as an effective means of skin regeneration to correct visual facial defects such as hyperpigmentation of various etiologies, fine wrinkles, sun damage, superficial scars, cotton wool and enlarged pores. It is also being used as a natural alternative approach to microdermabrasion and skin erosion, chemical peeling, CO ₂ laser ablation, and erbium laser ablation, which are physical procedures to improve skin conditions.

When the bone fragments come into contact with and rub against the skin layer, they are physically separated to reduce keratinocyte aggregation, and the keratin layer, sebum plug, and keratinocytes can be desorbed to promote regeneration of the skin.

On the other hand, since the fine needle powder is a natural substance obtained from nature, it exists in various saliva forms, of which one type is more advantageous for skin regeneration induction. However, it is difficult to collect uniform fine needle-like powder in a straight shape, and the cost of the fine needle-like powder is high. Therefore, fine needle powder obtained from algae or corals other than sponge animals may be mixed and used.

The microneedle powder has a needle-shaped structure, and the microneedle penetrates the skin layer to form a micro channel, so that the penetration effect of the applied cosmetic ingredient can be remarkably increased. In addition, the micropowder powder promotes skin regeneration of collagen which acts on an artificial wound healing, and increases skin tissue temperature to increase bioactivity.

Specifically, in the skin improvement effect, the supply of water is more important than the supply of nutrients to the skin. The needle-shaped structure of the microspheres induces moisture to be transferred to the dermal layer of the skin. It also activates circulation of oxygen in the skin to kill the anaerobic bacteria, acne bacteria.

In addition, fine needle powder can induce skin peeling and improve turn-over regeneration cycle of aged skin or acne skin. When the skin regeneration procedure using the existing peeling was the removal of the exfoliation and the regeneration, the peeling using the micropowder powder is the regeneration and the exfoliation.

Specifically, the fine needle powder penetrates into the pores and stimulates sleeping cells. As a result, it causes a slight inflammatory reaction to the skin. Generally, when a foreign body enters the body, the cell secretes regenerative substance to prepare for attack and defense, and the amount of oxygen is increased by increasing leukocytes, red blood cells and hemoglobin. Accordingly, as the nutrient supply to the cells becomes smooth, cell division rapidly increases, thereby inducing regeneration of the skin and normalizing the turn-over regeneration cycle of the skin.

In one embodiment, the micropowder powder may be from 0.5 to 10.0% by weight based on the total weight of the composition.

  If the content of the fine needle-like powder is less than 0.5% by weight, the effect of improving the penetration of the skin may not be sufficiently realized. If the content is more than 10.0% by weight, unnecessary fine needle powder may cause side effects or skin troubles.

The raw material of the fine needle powder itself may cause skin irritation due to imbalanced needle shape and other impurities, which may cause side effects such as allergy or inflammation to the skin. Therefore, it is difficult to apply to the skin only by the raw material itself, and it is difficult to realize the skin improvement effect. Thus, the raw material of the micropowder powder may undergo processing and purification processes.

The processing and refining process may be performed in the form of a powder by mixing the micropowder powder so that the micropowder powder has a uniform length and optionally adding other additives capable of improving the skin improving effect.

In one embodiment, the average particle length of the fine needle-like powder may be 60 to 500 탆. If the average particle length is less than 60 탆, it can not effectively penetrate into the skin to form pores. If it is 500 탆, the dispersibility may be lowered or excessive stimulation to the skin may occur.

On the other hand, the kind of the solvent used in the extraction step is not particularly limited, and various solvents may be used as needed. In addition, the extraction can be carried out using a conventional method in the art such as cold-rolling, warming, heating, and the like using the solvent.

The extract may be extracted with water, at least one solvent selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, and the alcohol has a concentration of 60 to 90% (v / v) ethanol.

That is, the extraction ratio may be different depending on the polarity of the solvent, and the ethanol is excellent in the selectivity in the extraction of the physiologically active substance of the natural raw material. Therefore, Can be implemented.

Particularly, the water and ethanol have different polarities, and the effective components extracted according to the respective polarities may be different from each other, so that the concentration of the ethanol can be appropriately controlled so that an optimum skin improving effect can be realized. However, if the concentration of ethanol is more than 90%, an adequate yield may not be realized. If the concentration is less than 60%, the effective ingredient showing skin improvement effect may not be extracted sufficiently. Therefore, Can be adjusted appropriately.

The combined extract is washed with water, dried, pulverized, and then subjected to extraction with a solvent having a volume of 8 to 12 times the weight of each raw material for about 1 hour to 24 hours using conventional methods such as circulation extraction, pressure extraction, And then filtering it. In addition, the extract may be obtained in powder form by an additional process such as vacuum distillation or lyophilization.

In addition, the extract may contain an extract obtained through a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

In one embodiment, the combined extract may be extracted for 12 to 18 hours at a temperature of 50 ° C to 60 ° C using the ethanol as a solvent.

On the other hand, if the extraction temperature is lower than 50 ° C, the extraction efficiency may be lowered and the active ingredient may not be eluted properly. If the extraction temperature is higher than 60 ° C, the active ingredient contained in the natural raw material may be deformed or lost at high temperature, Therefore, it is preferable to keep the extraction temperature within an appropriate range. If the extraction time is less than 12 hours, the active ingredient contained in the natural raw material may not be completely extracted. If the extraction time exceeds 18 hours, the content of the remaining active ingredient contained in the natural raw material is inadequate, It is preferable to control the extraction time appropriately.

In one embodiment, the cosmetic composition may be for acne improvement or skin cleansing. The cosmetic composition may effectively penetrate and act on the skin due to the fine needle as a result of which the skin improving effect can be remarkably improved.

According to one embodiment, the cosmetic composition is selected from the group consisting of softening longevity, nutritional lotion, nutritional cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder May be formulated into more than one.

The cosmetic composition may be appropriately blended with other ingredients within the range of not hindering the object of the present invention depending on the type of the active ingredient, the purpose of use, and the like in each formulation.

Also, depending on the quality or function of the final product, there may be used various additives such as a fatty substance, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, A surfactant, a stabilizer, a stabilizer, an antioxidant, an antioxidant, an antioxidant, a preservative, a barrier agent, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, And may further comprise adjuvants commonly used in cosmetics or dermatology, such as any of the other ingredients.

However, it is preferable that the adjuvant and the mixing ratio thereof are appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  1: Preparation of complex extract

After washing the tea tree leaves, rice bran, rhubarb, and aloe vera leaves, they were completely dried at room temperature and pulverized to obtain 100 g of each of the pulverized products.

(50% mass concentration) of a 10-fold volume of each of 100 g of each of the pulverized products of tea tree leaves, rice bran, rhododendron, and aloe vera leaves was subjected to immersion extraction at a temperature of 50 캜 for 12 hours. Each of the extracts was filtered by a filter, and the extracts obtained by repeatedly extracting the remaining raw materials three times in the same manner were mixed to complete a complex extract. The combined extracts were concentrated under reduced pressure at 30 DEG C and then lyophilized to give a solid.

Manufacturing example  2: Fine needle  Preparation of powder

Dried sponges ( Spongilla 800 g of a 0.5 N sodium hydroxide solution was added to 200 g of the natural sponge obtained in lacustris and mixed and pulverized by an ultrasonic process. After stirring, impurities were removed by centrifugation and 700 ml of purified water was added to remove soluble impurities and sodium hydroxide solution.

A small amount of sodium hydroxide was removed by chemical reaction with 800 ml of a 2% -L-ascorbic acid solution, and then the charged L-ascorbic acid solution was removed, followed by drying to obtain fine needle powder from which purified water was removed.

The combined extracts obtained in Preparation Example 1 and Preparation Example 2 and fine needle powder were put into a cosmetic composition and the skin improving effect on the cosmetic composition was verified.

Example  One

The combined extract of tea tree leaf extract, rice bran extract, rhododendron extract and aloe vera leaf extract obtained in Preparation Example 1 and 5 wt% of fine needle powder having an average length of 200 μm obtained in Production Example 2 were mixed and conducted A sample of Example 1 was prepared.

Example  2

A sample of Example 2 was prepared in the same manner as in Example 1, except that the content of the fine needle powder was adjusted to 7 wt%.

Example  3

A sample of Example 3 was prepared in the same manner as in Example 1, except that the content of the fine needle powder was adjusted to 2% by weight.

Example  4

A sample of Example 4 was prepared in the same manner as in Example 1 except that fine needle powder having an average length of 100 탆 was administered.

Example  5

A sample of Example 5 was prepared in the same manner as in Example 1, except that fine needle powder having an average length of 400 μm was administered.

Comparative Example  One

A sample of Comparative Example 1 was prepared in the same manner as in Example 1 except that fine needle powder was not included.

Comparative Example  2

A sample of Comparative Example 2 was prepared in the same manner as in Example 1 except that the content of the fine needle powder was adjusted to 0.1 wt%.

Comparative Example  3

A sample of Comparative Example 3 was prepared in the same manner as in Example 1 except that the content of the fine needle powder was adjusted to 15 wt%.

Comparative Example  4

A sample of Comparative Example 4 was prepared in the same manner as in Example 1, except that fine needle powder having an average length of 20 탆 was administered.

Comparative Example  5

A sample of Comparative Example 5 was prepared in the same manner as in Example 1 except that fine needle powder having an average length of 600 μm was administered.

A cosmetic sample was prepared as in Examples 1 to 5 and Comparative Examples 1 to 5, and the content thereof is shown in Table 1 below.

[Content (% by weight)] division Example
One Example
2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Fine needle powder 5.0 7.0 2.0 5.0 5.0 - 0.1 15 5.0 5.0 Average length of fine needle powder (mu m) 200 200 200 100 400 - 200 200 20 600 Complex extract 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 1,3-butylene glycol 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Oleyl alcohol 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Polysorbate 20 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 ethanol 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 Benzophenone-9 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance Balance Balance Balance Balance Balance

Experimental Example  1: Assessment of cytotoxicity

The toxicity of the samples obtained in Examples 1 to 5 and Comparative Examples 1 to 5 on cells was measured. The samples obtained in the above Examples 1 to 5 and Comparative Examples 1 to 5 were suspended in purified water to give concentrations of 100, 50 and 25 μg / mL, respectively, and cell viability was measured by the following method.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

HDF (Human Dermal Fibroblast) cells were seeded in a 96-well plate at a concentration of 1 × 10 ⁴ cells / well and stabilized at 37 ° C and 5% CO ₂ for 24 hours. The samples obtained in Examples 1 to 5 and Comparative Examples 1 to 5 were treated for each concentration and cultured for 24 hours. After the medium was removed, MTT was treated at a concentration of 5 mg / mL and cultured for 2 hours. 100 μL of DMSO (dimethyl sulfoxide) was added, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was observed at all concentrations of each sample. This suggests that the complex extract is harmless to the human body and has excellent safety.

Experimental Example  2: Evaluation of skin soothing effect

After inducing skin irritation through the tape stripping method on the inner part of the arm of 50 women with stimulant-sensitive skin among the 19 to 26 year-old women, the above-mentioned Examples 1 to 5 and Comparative Example The samples 1 to 5 were applied twice daily for 1 month to the face and the first half. The degree of improvement was determined according to the opinions of the user such as stinging, itching, redness, and erythema. The results are shown in Table 2 below.

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 1 week + + + + + ± ± ± ± ± 2 weeks ++ ++ ++ ++ ++ ± ± ± ± ± 3 weeks +++ ++ ++ ++ ++ ± ± ± ± ± 4 weeks +++ +++ +++ +++ +++ + + - + -

<Evaluation Criteria>

+++: very good improvement effect / ++: considerable improvement effect / +: slight improvement effect / ±: no improvement effect but not deterioration / -: not improvement effect but rather weakness

As shown in Table 2, it was confirmed that the samples (Examples 1 to 5) including the mixed extract and the micropowder powder according to the present invention had remarkably excellent skin soothing effect as compared with the samples of Comparative Examples 1 to 5.

On the other hand, in the case of the comparative example, the improvement effect was insignificant. However, in the case of the sample of Comparative Example 3 in which the fine needle powder was excessively administered and the case of Comparative Example 5 in which the fine needle powder with the necessary length was administered, It was confirmed that the skin trouble was exacerbated by stimulation.

Experimental Example  3: Evaluation of skin moisturizing effect

Fifty of the women aged 19 to 26 years were subjected to Chromameter CM825 to measure the amount of skin moisturized before use of the test subjects, and then the samples of the formulated Examples 1 to 5 and Comparative Examples 1 to 5 were applied daily for 2 And then applied to the face and the first half for one month to re-measure the skin moisturizing amount. The measured values were expressed by three average values excluding the maximum value and the minimum value, and the results are shown in Table 3 below.

[Arbitrary unit] division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Before use 52.18 52.18 52.18 52.18 52.18 52.18 52.18 52.18 52.18 52.18 after use 74.64 71.93 71.51 71.39 64.84 56.77 60.19 57.85 59.12 59.53 Growth rate
(%) 43.04 37.85 37.04 36.81 24.26 8.22 15.35 10.87 13.30 14.09

As shown in Table 3, the samples (Examples 1 to 5) containing the mixed extract and the micropowder powder according to the present invention had remarkably superior skin moisturizing effect than the lotion containing the samples of Comparative Examples 1 to 5 Respectively.

On the other hand, it was confirmed that the samples of Comparative Examples 1 to 5 in which the fine needle powder was not contained or out of the appropriate range had a very low absorption rate to the skin as compared with the samples of Examples 1 to 5, and the skin moisturization increase rate was remarkably low.

Experimental Example  4: Evaluation of acne improvement effect

The samples of the formulations of Examples 1 to 5 and Comparative Examples 1 to 5 were applied twice daily for 1 month to the face and the first half of 50 women with acne among 19 to 26 year old women. The degree of improvement was determined and the results are shown in Table 4 below.

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 1 week + + + + + ± ± ± ± ± 2 weeks ++ ++ ++ ++ ++ ± ± ± ± ± 3 weeks +++ ++ ++ ++ ++ ± ± ± ± ± 4 weeks +++ +++ +++ +++ +++ + ± - ± -

<Evaluation Criteria>

+++: very good improvement effect / ++: considerable improvement effect / +: slight improvement effect / ±: no improvement effect but not deterioration / -: not improvement effect but rather weakness

As shown in Table 4, it was confirmed that the samples (Examples 1 to 5) containing the mixed extract and the micropowder powder according to the present invention had remarkably excellent acne improvement effect as compared with the samples of Comparative Examples 1 to 5.

In particular, it was confirmed that the sample of Comparative Example 1, which did not contain fine needle powder, had significantly reduced acne improvement effect as compared with the samples of Examples 1 to 5, and the sample of Comparative Example 3 in which the micropowder powder was excessively administered In the case of Comparative Example 5 in which the fine needle powder having a length of more than 5 mm was administered, excessive skin irritation was given and it was confirmed that the skin trouble was deteriorated.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (8)

20 to 40 parts by weight of a tea tree leaf extract, 10 to 20 parts by weight of a rice bran extract, 10 to 20 parts by weight of a rhododendron extract, and 10 to 20 parts by weight of an aloe vera leaf extract; And a fine needle powder as an active ingredient.
The method according to claim 1,
The fine needle powder is obtained from a sponge animal.
3. The method of claim 2,
The sponge animal is a Spongilla &lt; RTI ID = 0.0 &gt; lacustris .
The method according to claim 1,
Wherein an average length of the fine needle-like powder particles is 60 to 500 占 퐉.
The method according to claim 1,
Wherein the fine needle powder is 0.5 to 10.0% by weight based on the total weight of the composition.
delete delete 6. The method according to any one of claims 1 to 5,
A cosmetic composition comprising at least one selected from the group consisting of softening agents, nutritional lotions, nutritional creams, massage creams, essences, ampoules, gels, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, sprays and powders.