KR101727979B1 - An unmanned environmental sample collection system - Google Patents

Abstract

The present invention relates to a moving sample collection system (apparatus) capable of moving to a specific point unattended or remotely to collect a sample, and more particularly to a moving sample collection system It is possible to collect the environmental sample at the correct target point by moving the surface of the water or the ground by the active control signal to set the target point. Based on the type of sound received, the image, and the thermal image, And an unmanned environmental sample collection system (apparatus) capable of setting, moving, and collecting samples.

Description

[0001] AN UNMANNED ENVIRONMENTAL SAMPLE COLLECTION SYSTEM [0002]

The present invention relates to a moving sample collection system capable of moving to a specific point unattended or remotely to collect a sample, and more particularly to a moving sample collection system which actively sets a target point based on a remote control signal or based on a received sound or image signal It is possible to collect sample of environmental samples at the precise target point by moving the surface of the water or the ground by the active control signal, and to determine the object by the kind of sound to be received, image or thermal image, And an unmanned environmental sample collection system capable of collecting samples.

There is a steady increase in demand for environmental monitoring in systems that collect and collect waterborne pathogens present in large-volume environmental samples such as rivers, lakes, and reservoirs. For example, a method for collecting water-borne pathogenic substances present in an existing environmental sample is a method in which a person directly moves to a corresponding site and collects environmental sample samples or uses an apparatus for collecting environmental sample samples at fixed points as in the following patent documents Respectively.

<Patent Literature>

Patent No. 10-0387362 (Registered on May 30, 2003) "Real-time Water Quality Management and Automatic Sampling System for Remote Water Pollution Source"

However, the method of collecting waterborne pathogens present in existing environmental samples is very difficult to accurately collect samples at the locations where environmental sample samples are located in large areas such as rivers, lakes and reservoirs. Thus, there is a growing need for a mobile sample collection system that can be moved to a specific location unattended or remotely to capture environmental sample samples of the site.

In addition, in the conventional method of collecting environmental sample samples, an environmental sample (liquid) is passed through a micro-filter to induce a pathogen present in the sample to adhere to the filter, and then the filter is separated, Adsorption-elution methods have been used, but this has been particularly problematic for certain pathogens such as avian influenza (<10%). This low collection rate is critical to the monitoring of infectious pathogens (avian influenza, etc.) for which confirmation is crucial and needs to be improved. To solve this problem, many devices with high collection rate and concentration ratio have been developed using micro nano-based technology , It is impossible to treat a large-capacity environmental sample at a remarkably low processing rate (< 1 mL / min).

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems,

It is an object of the present invention to provide a moving sample collection system capable of moving to a specific point unattended or remotely to collect a sample, and more particularly to a moving sample collection system capable of actively detecting a target point based on a remote control signal or based on a received sound or image signal And to provide an unmanned environmental sample sampling system capable of collecting environmental sample samples at accurate target points by moving the water surface or the ground by the set active control signal.

It is another object of the present invention to provide an unmanned environmental sample sample collection system capable of discriminating the type of sound to be received or an object of an image and setting an accurate target point on the basis thereof to move and collect samples.

It is another object of the present invention to provide an unmanned environmental sample collection system capable of reducing an error to a target point by using a navigation image or a thermal image of a surrounding terrain.

It is still another object of the present invention to provide a method for rapidly collecting and concentrating waterborne pathogens from environmental samples in water at a high collection rate and concentration ratio and utilizing a modular waterborne pathogen concentration module to collect and concentrate Which is capable of controlling the environment of the unmanned environmental sample.

It is another object of the present invention to provide an unmanned environmental sample collection system which can improve the accuracy of pathogen collection by approaching an area where a subject object (bird, etc.) having a high risk of pathogen infection or coagulation is present using thermographic image data .

In order to achieve the object of the present invention, the unmanned environmental sample collection system includes the following configuration.

According to an embodiment of the present invention, there is provided an unmanned environmental sample collection system comprising: a moving unit for movement on a water surface or a ground; A signal processing unit for sensing a surrounding environment of an area to be captured of the environmental sample and outputting information; A control unit for controlling the moving unit according to an active control signal generated by analyzing the information output from the signal processing unit or a remote control signal output by a user so as to move to a target point; And a collecting and storing unit for collecting and storing environmental sample samples at one side of the moving unit to collect environmental sample samples by moving to a target point.

In the unmanned environmental sample sample collecting system according to another embodiment of the present invention, the signal processing unit may include a GPS module for outputting a GPS position signal, and the control unit may be configured to receive, based on the GPS information included in the remote control signal And a remote control module for controlling the moving unit so as to be able to move to a specific position.

In the unmanned environmental sample sample collecting system according to another embodiment of the present invention, the signal processing unit may include a sound module for outputting ambient sounds or an image module for outputting a peripheral image, And an active control module for controlling the moving unit to move to a position where a specific sound is collected or a specific object is detected based on an output sound signal or an image signal output from the image module.

In the unmanned environmental sample sample collecting system according to another embodiment of the present invention, the active control module may specify a type of sound through a sound output from the sound module, Based control signal generation module for generating a control signal for moving to a position where the object is located when the number of specified objects is greater than or equal to a predetermined value, And an image-based control signal generation module for generating a control signal.

In the unmanned environmental sample sample collection system according to another embodiment of the present invention, the signal processing unit may further include a navigation receiving module for outputting a navigation image related to the peripheral terrain and a thermal image receiving module for outputting thermal image .

In the unmanned environmental sample sample collecting system according to another embodiment of the present invention, the active control module may be configured such that a position of a target object at night or a target object moves based on a thermal image output from the thermal image receiving module A thermal image-based control signal generation module for detecting a position of an environment sample sample that has just been discharged and generating a control signal for moving to a corresponding position; Based control signal generation module or the image-based control signal generation module or the thermal-image-based control signal generation module according to an embodiment of the present invention.

In the unmanned environmental sample sample collection system according to another embodiment of the present invention, when the temperature of the object is determined to be higher than a predetermined temperature by analyzing the deteriorated image outputted from the thermo-image module, And a temperature analysis signal generation module for generating a control signal that moves to the position of the target object in consideration of the infected object.

In the unmanned environmental sample collection system according to another embodiment of the present invention, the collection and storage unit includes a plurality of modularized water-borne pathogen enrichment modules, and the environmental samples collected in the field are collected by a plurality of water- And the water-borne pathogen is concentrated at a high concentration.

In the unmanned environmental sample collection system according to another embodiment of the present invention, the enrichment module includes an enrichment unit for concentrating the water-borne pathogen in an environmental sample flowing at a constant rate using an electric field and a micro-unit filter membrane .

In the unmanned environmental sample sample collection system according to another embodiment of the present invention, the concentration module through which the environmental sample first passes among the plurality of concentration modules may further include a pretreatment filter that removes suspended substances having a predetermined size or larger in advance .

In the unmanned environmental sample sample collecting system according to another embodiment of the present invention, the enrichment unit may include an electrode for generating an electric field at upper and lower portions of a flowing portion through which the introduced environmental sample flows, And a buffer flow unit positioned on upper and lower sides of the flow unit to allow an electric field to be transmitted to the flow unit.

The present invention can obtain the following effects by the above-described embodiment, the constitution described below, the combination, and the use relationship.

The present invention relates to a moving sample collection system capable of moving to a specific point unattended or remotely to collect a sample, and more particularly to a moving sample collection system which actively sets a target point based on a remote control signal or based on a received sound or image signal The active control signal can move the surface of water or the ground to collect the environmental sample at the correct target point.

The present invention has the effect of determining the type of sound to be received or an object of an image, setting an accurate target point on the basis of the object, and moving and collecting samples.

The present invention has an effect of reducing an error to a target point by using a navigation image or a thermal image of a surrounding terrain.

The present invention can rapidly collect and concentrate water-borne pathogens from environmental samples in the water at a high collection rate and concentration ratio, and can utilize a modular water-borne pathogen concentration module to adjust the collection and concentration ratio as needed Effect.

The present invention has the effect of enhancing the accuracy of pathogen trapping by approaching an area where a target object (algae, etc.) having a high risk of pathogen infection or coagulation is present using the thermographic image data.

1 is a schematic perspective view of an unmanned environmental sample collection system according to an embodiment of the present invention;
BACKGROUND OF THE INVENTION 1. Field of the Invention [0001]
FIG. 3 is a block diagram showing a detailed configuration of the active control module of FIG. 2; FIG.
FIG. 4 is a schematic configuration view of the collecting storage section of FIG. 2; FIG.
FIG. 5 is a schematic view showing a detailed configuration and an operation process of the concentrating part of FIG. 4; FIG.
FIG. 6 is a fluorescence microscope photograph of an operating state of the enrichment unit according to an embodiment of the present invention. FIG.
7 is a graph showing the operation results of the enrichment unit according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the unmanned environmental sample collection system according to the present invention will be described in detail with reference to the accompanying drawings. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. Throughout the specification, when an element is referred to as "including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

1 to 7, the collecting system (apparatus) comprises a moving part 1 for movement on the water surface or on the ground, A signal processing unit 2 located at one side of the moving unit 1 for sensing the surrounding environment and outputting information, a remote control signal generated by the user or information output from the signal processing unit 2 A control unit 3 for controlling the moving unit 1 according to the generated active control signal so as to move to a target point for sampling an environmental sample, And a collecting storage unit 4 for collecting and storing the environmental sample sample when it reaches the target point, and collect the environmental sample sample by moving to the target point. The environmental sample sampling system can communicate with a control system (not shown) to exchange information. In the following description, the signal processing unit 2 and the control unit 3 are located together in the moving unit 1, but the signal processing unit 2 and the control unit 3 are located separately from the moving unit 1 It is also possible to control the moving part 1 to move it to a specific position, and then to collect the environmental sample sample through the collecting low temperature part 4.

The moving unit 1 is a moving means for allowing the collection system according to the present invention to collect environmental samples (feces, carcass, water, etc.) at a target point while moving, and various known means can be used have. In particular, the collection system of the present invention is capable of collecting fecal samples or water samples of migratory birds while moving in rivers, rivers, lakes, reservoirs, etc. in order to detect avian influenza viruses and the like, (For example, a structure in which a track (a caterpillar) or a wheel is mounted on a lower portion of a boat, for example) capable of moving amphibiously .

The signal processing unit 2 is located at one side of the moving unit 1 and senses the surrounding environment to output information. In particular, the signal processing unit 2 outputs a GPS position signal required for the collection system according to the present invention to move to a specific position A navigation module 22 for outputting a navigation image related to the surrounding terrain, a sound module 23 (using sound detection means or the like) for outputting ambient sounds in the area where the collection system is located, The image module 24 (the concept including the camera module as the image detection means) for outputting the peripheral image of the area in which the collection system is located, the thermal imaging module 25 for outputting the thermal image around the collection system , Using an infrared camera or the like), and the like.

The control unit 3 controls the moving unit 1 according to an active control signal generated by analyzing a remote control signal generated by a user or information output from the signal processing unit 2 to collect an environmental sample The remote control module 32, the active control module 33, the sensing information providing module 34, the collecting control module 35, and the like, It can be operated remotely as well as automatically analyze the collected information.

The transceiving module 31 exchanges information with the moving unit 1, the signal processing unit 2, the collecting storage unit 4, and the control system.

The remote control module 32 transmits the remote control signal to the mobile unit 30 so that the remote control module 32 can move to a specific position based on the GPS information included in the remote control signal according to the remote control signal transmitted from the control system and output from the transmission / (1), the remote control signal transmitted remotely from the control system or the like is analyzed and the collection system is moved to a point matching the corresponding signal.

In another example, the control unit 3 analyzes the information output from the signal processing unit 2, not the remote control by the control system, and automatically controls the moving unit 1 to output an active control signal, For this purpose, a sound signal output from the sound module 23, a video signal output from the video module 24, or a thermal video signal output from the thermal imaging module 25 may be used to generate a specific sound And an active control module 33 for controlling the moving unit 1 so as to move to a position where a specific object is detected or a position where a specific object is detected.

Specifically, the active control module 33 specifies the type of sound through the sound output from the sound module 23, and generates a control signal to move to a position where the sound is emitted when the specified sound is equal to or greater than a reference value A sound based control signal generation module 331 for generating a control signal for specifying an object type through an image output from the image module 24 and a control signal for moving to a position where the object exists if the number of specified objects is equal to or greater than a reference value Based on the thermal image output from the thermo-image module 25, the position of the object of the night or the position of the object of the environmental sample A thermal image based control signal generation module 333 for detecting a position and generating a control signal for moving to a corresponding position; A temperature analysis signal generation module 334 for generating a control signal for moving to a position of the target object when it is determined that the temperature of the target object is higher than a predetermined temperature, and a peripheral terrain navigation Based control signal generation module 331 or the image based control signal generation module 332 or the thermal image based control signal generation module 333 or the temperature analysis signal generation module 334 based on the image And a navigation control module 335 for reducing an error with respect to the target position.

For example, when it is necessary to collect fecal samples of migratory birds from reservoirs in order to detect avian influenza virus, it is important to determine a place where many fecal samples of migratory birds are located in a wide reservoir area and collect samples at the place, The sound-based control signal generation module 331 specifies a sound of migratory birds among sounds received in the vicinity of the collection system, selects a region where the sound is detected above a reference value, and generates a control signal to move to the corresponding region, The system will be able to collect environmental sample samples in areas where the migratory birds were located.

In addition, the image-based control signal generation module 332 may generate the image-based control signal in the vicinity of the collection system, for example, when it is necessary to collect fecal samples of migratory birds in a reservoir or the like as described above, (Eg, moving images of migratory birds caught by the camera), the control system generates a control signal to move the birds to the corresponding area by selecting the movement of the birds and the location where the birds are staying, The infrared image-based control signal generation module 333 can acquire a sample image based on the thermographic image (infrared image, etc.) to be photographed, To generate control signals to move to the area, or at the time when migratory birds have moved, Sensing the position of the feces to be possible to be able to collect the environmental test sample to generate a control signal to move in the area.

In addition, the temperature analysis signal generation module 334 may be used to determine the locations where fecal samples of the migratory birds are likely to be located, for example, when fecal samples of the migratory birds must be collected at the reservoir or the like, When it is determined that the temperature of the algae is higher than the predetermined temperature based on the captured deterioration image, it is possible to collect the environmental sample by generating the control signal so that the algae are moved to the location where the algae are located .

The navigation control module 335 may generate the sound based control signal generation module 331 or the image based control signal generation module 332 or the image based control signal generation module 332 based on the peripheral terrain navigation image output from the navigation module 22, The target position of the control signal generated by the control signal generation module 333 or the temperature analysis signal generation module 334 can be reduced to an error in moving to the target point by comparing the target position with the accurate topography.

The sensing information providing module 34 controls the sensing information of the surrounding environment output to the signal processing unit 2 to be transmitted to the control system. The collection control module 35 controls the operation of the collection storage unit 4 when the collection system is located at a target point by the remote control module 32 or the active control module 33. [

The collection and storage unit 4 is configured to collect and store environmental sample samples when they reach a target point on one side of the moving unit 1. The collection and storage unit 4 stores a solid sample ) And / or a liquid sample (water) are collected and stored. Hereinafter, the detailed configuration of the collecting storage unit 4 for collecting liquid samples will be described in detail.

The collection and storage unit 4 includes a plurality of concentration modules 41 for collecting and concentrating water-borne pathogens in a liquid sample (water), and an environmental sample collected in the field is supplied to the plurality of concentration modules 41 ), The water-borne pathogen is concentrated to a high concentration. The enrichment modules 41 are connected in series and / or in parallel. When the enrichment modules 41 are connected in series as shown in FIG. 4, the environmental sample passes through the enrichment modules 41, Is increased. For example, assuming that each enrichment module 41 is capable of 20-fold concentration of the water-borne pathogen relative to the environmental sample and that the three enrichment modules 41 are connected in series, the water- Is concentrated 20 ³ times as much as the input environmental sample. Although not shown, when the concentrating modules 41 are connected in parallel, the collection rate (total throughput) of the water-borne pathogen can be increased. Accordingly, the various numbers of the concentration modules 41 can be arranged in series and / or parallel to easily obtain the necessary concentration ratio and throughput. Since the environmental sample collected at the site of the river, river, reservoir or the like flowing into the collecting and storing unit 4 includes many foreign substances, the mesh net 42 (mesh) 42 for removing foreign substances is disposed at the front end of the first concentration module 41a. ). &Lt; / RTI &gt;

The enrichment module 41 includes a fluid power supply unit 411 for supplying power to the enrichment unit 412 through the enrichment unit 412 at a constant speed, And an enrichment unit 412 for fractionally concentrating the water-borne pathogen in the environmental sample. One fluidizing power supply unit 411 and one enrichment unit 412 are modularized to form one enrichment module 41. If necessary, the fluidity supply unit 411 may supply only the specific enrichment module 41 It is also possible to include it. The fluid supply unit 411 may use various means capable of providing a power for moving the environmental sample, for example, a hydraulic pump. The concentration module 41a through which the environmental sample passes first among the plurality of concentration modules 41 may further include a pretreatment filter 413 that removes the suspended substances having a predetermined size or more in advance. 4, a preliminary filter 413 (prefilter) is disposed before the environmental sample introduced into the concentrated storage section 4 is injected into the concentration section 412a of the concentration module 41a for the first time, , Preferably a filter in the order of a few micrometers) to remove unnecessary suspended matter or foreign matter from the front end before entering the thickening section 412a to thereby improve the efficiency of the fractionation concentration action of the water-borne pathogen in the thickening section 412a .

The enrichment unit 412 is configured to concentrate the water-borne pathogen in the environmental sample moving by the power provided by the flow-power supply unit 411, and is configured to concentrate the water-borne pathogen at a constant rate by using the electric field and the micro-scale filter membrane 4123 Water-borne pathogens are fractionally concentrated in flowing environmental samples.

The enrichment unit 412 includes a flow unit 4121 through which the introduced environmental sample flows and an electrode 4122 located on the upper and lower sides of the flow unit 4121 to generate an electric field, A filter membrane 4123 for separating the space of the fluid portion 4121 at a constant height along the longitudinal direction, a cation exchange membrane 4124 respectively located on the upper and lower sides of the fluid portion 4121, the cation exchange membrane 4124, And a buffer flow portion 4125 formed between the electrode 4122 and the electrode 4122 to move the buffer solution.

The flow unit 4121 is configured such that the introduced environmental sample is moved. The fluid unit 4121 is divided into a space at a predetermined height by the filter membrane 4123, an environmental sample flows into the lower part, And a pathological channel 4121b through which the water channel 4121a is discharged after the water channel 4121 is removed and the water channel 4121a which is introduced into the waste channel 4121a through the filter channel 4123 is concentrated and discharged. do. As shown in Fig. 6 (a), the fluids in the waste channel 4121a and the pathogen channel 4121b flow in the same direction (Parallel-flow operation) The fluid in the waste channel 4121a and the fluid in the pathogen channel 4121b may flow in different directions as shown in FIG.

The electrodes 4122 are located on the upper and lower sides of the flow portion 4121, respectively, so that an electric field is generated in the concentration portion 412.

The filter membrane 4123 is configured to separate the space of the fluid portion 4121 at a predetermined height along the longitudinal direction of the fluid portion 4121 and to allow passage of water pathogens such as viruses and bacteria lt; RTI ID = 0.0 &gt; um. &lt; / RTI &gt; A substance larger than several um in the environmental sample which has not been removed by the mesh network 42 and the pretreatment filter 413 can not pass through the filter membrane 4123 and can not pass through the waste channel 4121a of the fluid section 4121 It remains.

The cation exchange membranes 4124 are located on the upper and lower sides of the flow portion 4121, respectively, so that ions can be moved by the electrodes 4122.

The buffer flow portion 4125 is formed between the cation exchange membrane 4124 and the electrode 4122 to move the buffer solution. As the buffer solution, for example, NaCl, KCl, or the like may be used. Buffer flow units 4125 are positioned on upper and lower sides of the flow unit 4121 through which the environmental sample flows so that an electric field is transmitted to the concentration unit 412, So that the reaction product due to the electrochemical reaction occurring in the reaction vessel can not be influenced by the concentration.

A method for concentrating water-borne pathogens such as viruses and bacteria using the enrichment unit 412 having the above-described structure will be described below. In the enrichment unit 412 constructed as shown in FIG. 5, through the inlet of the waste channel 4121a When an environmental sample is introduced and power is supplied through the electrode 4122, ion concentration polarization occurs in the lower cation exchange membrane 4124a, and all charged substances such as water-borne pathogens are directed upward The water-permeable pathogen having a size smaller than that of the filter membrane 4123 located at a predetermined height of the fluid 4121 passes through the filter membrane 4123 and collects in the pathology channel 4121b, Only foreign matter or other particles remain in the discharge portion of the waste channel 4121a, and it becomes possible to concentrate the water-borne pathogen in the environmental sample. The concentration unit 412 adjusts the concentration of the target water-borne pathogen by adjusting the voltage applied to the electrode 4122, the pore size of the filter membrane 4123, the flow rate of the waste channel 4121a and the pathogen channel 4121b, Can be adjusted. 5, the other conditions are the same, the pore size of the filter membrane 4123 is 1 mu m, the flow rate of the pathogen channel 4121b is 20 mu L / min, 4121a) at a flow rate of 280 μL / min, and an environmental sample containing less than 1 μm and fluorescent dye-stained water-borne pathogens (fluorescent particles) was supplied and supplied at a constant voltage, It was possible to concentrate human pathogens. 6 (a) is a photograph of a part of the thickening part 412 taken under a fluorescent microscope under the above conditions. It can be seen that the pathogen channel 4121b shines brightly on the waste channel 4121a, It can be seen that fluorescent particles have been enriched in pathogen channel 4121b. 6 (b) is a result of experiment under different conditions from FIG. 6 (a). It can also be seen that the pathogen channel 4121b shines brightly on the waste channel 4121a in FIG. 6 (b) , It can be seen that the fluorescent particles have been concentrated in the pathogen channel 4121b. 7 is a graph showing the fluorescent intensity measured according to the distance A-A 'of the thickening part 412 as shown in FIG. 6 (a) It can be confirmed that fluorescence particles can be enriched up to 20 times when a voltage is supplied for 150 seconds in comparison with the case (0 sec).

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Should be interpreted as belonging to the scope.

1: moving part 2: signal processing part 21: GPS module
22: navigation module 23: sound module 24: video module
25: thermography module 3: control unit 31: transmission / reception module
32: remote control module 33: active control module 34: sensing information providing module
35: Capture control module 331: Sound based control signal generation module
332: image-based control signal generation module 333: thermal image-based control signal generation module
334: Temperature analysis signal generation module 335: Navigation control module 4: Collection storage unit
41: enrichment module 42: mesh network 411: fluid power supply
412: Enrichment section 413: Pretreatment filter 4121:
4122: Electrode 4123: Filter membrane 4124: Cation exchange membrane
4125: buffer flow unit 4121a: waste channel 4121b: pathogenic channel

Claims (11)

A moving part for movement on the water surface or the ground;
A signal processing unit for sensing a surrounding environment of an area to be captured of the environmental sample and outputting information;
A control unit for controlling the moving unit according to an active control signal generated by analyzing the information output from the signal processing unit to move to a target point;
And a collecting and storing unit for collecting and storing the environmental sample, which is located at one side of the moving unit,
Wherein the signal processing unit includes a sound module for outputting ambient sound and a thermal imaging module for outputting a thermal image,
Wherein the control unit controls the moving unit to move to a position where a specific sound is collected based on a sound signal output from the sound module and a position where an object specified based on the thermal image output from the thermo-picture module is located, Module,
Wherein the active control module includes a sound based control signal generation module that specifies a type of sound through a sound output from the sound module and generates a control signal that moves to a position where the sound is collected when the specified sound is equal to or greater than a reference value; Based on the thermal image output from the thermal imaging module, a thermal image-based control signal generation module that detects a position of a target object at night or a position of an environmental sample sample discharged from a target object and generates a control signal to move to the corresponding position &Lt; / RTI &
The collection and storage unit includes a plurality of modular waterborne pathogen enrichment modules so that the environmental samples collected in the field sequentially pass through the plurality of waterborne pathogen enrichment modules to concentrate the waterborne pathogen at a high concentration, Collecting environmental sample samples,
The concentration module includes an enrichment unit for concentrating the water-borne pathogen in an environmental sample flowing at a constant rate using an electric field and a micro-unit filter membrane. The enrichment module, through which the environmental sample first passes among the plurality of concentration modules, Further comprising a pretreatment filter for removing the floating matters in advance,
The concentrating unit includes a filter membrane for placing an electrode for generating an electric field in the upper and lower portions of the flow portion through which the introduced environmental sample flows and separating the space of the flow portion at a predetermined height along the longitudinal direction of the flow portion, And a buffer flow unit for allowing an electric field to be transmitted to the flow unit.
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