KR101724769B1 - Methylobacterium aquaticum for Odorless Bioflim-Coated Evaporator Core and uses thereof - Google Patents

Abstract

The present invention relates to a microbial methyl bromide microorganism used for coating with an odorless biofilm which does not cause odor in an air conditioning apparatus Aqua tikum (Methylobacterium The present invention relates to an odorless EVA core which does not cause odor in an air conditioner coated with a biofilm using the same.
The odorless microorganisms and the odorless eva cores of the present invention can inhibit the growth of microorganisms causing odors in the air conditioner, thereby providing a pleasant indoor environment.

Description

Methylobacterium aquaticum for use in odorless EVA core coatings and uses thereof Methylobacterium aquaticum for Odorless Bioflim-Coated Evaporator Core and uses thereof -

The present invention relates to an odorless EVA core coated with a biofilm as an odorless microorganism in order to inhibit the growth of microorganisms causing odor in an air conditioner, a method for producing the same, and a method for producing microbial methylobacterium It is about the aquaticum .

Clean air is recognized as the basis for human health and well-being. For example, it has two important factors that can lead to unsatisfactory indoor air quality in an enclosed building. One is the building itself, which causes large amounts of indoor air pollutants to be removed or diluted, and the other is the generation of odors generated by human activities.

The air conditioning system is a system that optimizes the indoor environment by lowering the indoor temperature with the aim of harmonizing air temperature, humidity, air flow and cleanliness. These air conditioning systems are increasing in penetration rate due to the improvement of living standards. There have been many improvements in the basic functions due to the increase of the air conditioning system, but there are still many problems to solve due to the environmental aspect for the indoor air quality.

In air conditioning systems, air-conditioner odor is known to be caused by odor of mold and bacteria, but specific data on how specific fungi and germs secrete certain metabolites are not yet known .

In the structure of the air conditioner, all the air passing through the blower passes through the evaporator core. When heat exchange is performed between the cold refrigerant and the air, condensation condensation phenomenon occurs on the evaporator surface depending on the temperature difference. Is provided with a good environment for fungi and bacteria to form and propagate. When fungi and bacteria are proliferated on the EVA core exposed to the outside air, volatile organic compounds (mVOCs) of the microorganisms are generated as the metabolites of the bacteria that have proliferated on the surface of the EVA core, and air passing through the EVA core is blown into the room The volatile organic compounds generated by the microorganisms may expose the room to odors caused by fungi and bacteria during long-term use.

The EVA core surface, which generates bad odors, is covered with biofilm according to long-term use. They are composed of bacteria, cell clusters and EPS. EPS is composed of protein, polysaccharide, polyuronic acid, Nucletic, and lipid. On the surface of EVA core, various bacteria and fungi multiply as biofilms and they release metabolites (mVOCs) by microorganisms as metabolites. This is known to be among the many factors of air conditioner odor.

Accordingly, the present inventor has proposed a biofilm formed of an odorless or fragrant microorganism on the surface of Eva core in order to prevent deposition and propagation of bacteria and fungi on the surface of Eva core, Coated with a biofilm layer coated on the surface of the biofilm.

However, in the above-mentioned invention, it is not confirmed whether any bacteria are odorless microorganisms.

It should be understood that the foregoing description of the background art is merely for the purpose of promoting an understanding of the background of the present invention and is not to be construed as adhering to the prior art already known to those skilled in the art.

Accordingly, the present inventors have studied whether any microorganisms can be used as odorless EVA cores when they are coated with biofilm as odorless microorganisms in order to inhibit the growth of odor-causing microorganisms in an air conditioner. As a result, Microbial methyl bromide Aquaticum was identified.

The present invention relates to a microbial methyl bromide microorganism used for coating with an odorless biofilm which does not cause odor in an air conditioning apparatus To provide an aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) and for that purpose.

The present invention relates to the use of methyl < AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) which does not cause odor in an air conditioner coated with biofilm.

The present invention

(i) On the surface of the EVA core , methylobacterium Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) applying the medium favorable to the propagation;

(ii) a methyl on the surface of the medium is applied tumefaciens Affixing the aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P); And

(iii)Methyl bromide Aquatiquem HKMC-1 (Methylobacterium aquaticum HKMC-1) (KCCM11325P) to form a biofilm;

And an object of the present invention is to provide a method for producing an odorless eva core which does not cause odor in the air conditioner.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, there is provided a microbial microbacterium, which is used for coating with an odorless biofilm that does not cause odor in an air conditioner, AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P).

According to a further aspect of the invention there is provided a bacterium methyl Solarium AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) provides odorless EVA cores that do not cause odors in the air conditioner coated with biofilm.

According to another aspect of the present invention,

(i) On the surface of the EVA core , methylobacterium Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) applying the medium favorable to the propagation;

(ii) a methyl on the surface of the medium is applied tumefaciens Affixing the aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P); and

(iii) the methylobacterium AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) to form a biofilm;

The present invention provides an odorless EVA core manufacturing method that does not cause odor in the air conditioner including the above-described odor eliminating device.

The features and advantages of the present invention are as follows.

(i) The present invention relates to a microbial methyl bromide microorganism used for coating with an odorless biofilm which does not cause odor in an air conditioning apparatus Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) and that do not use them to induce within the odor was coated with a biofilm air conditioner relates to an odorless EVA core.

(ii) The odorless microorganisms and the odorless eva cores of the present invention can inhibit the propagation of odorous microorganisms in the air conditioner to remove odors in the room.

Figure 1 Dipping in a nutrient medium to the sterile aluminum pin to include dust and bacterium with later methyl Solarium AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P).

According to one aspect of the present invention, there is provided a microorganism used for coating with an odorless biofilm which does not cause odor in an air conditioning apparatus,Methyl bromide Aquatiquem  (Methylobacterium aquaticum). More preferably, the microorganism used for the coating as the odorless biofilm isMethyl bromide Aquatiquem HKMC-1 (Methylobacterium aquaticum HKMC-1) (KCCM11325P). exampleMethyl bromide AquatiquemOn November 14, 2012 at the Korea Microorganisms Conservation Center. remindMethyl bromide Aquatiquem HKMC-1 (Methylobacterium aquaticum In addition to HKMC-1) (KCCM11325P)Methyl bromide Brakiatum HKMC-2 (Methylobacterium brachiatum HKMC-2) (KCCM11326P),Methyl bromide Platani HKMC-3 (Methylobacterium platani HKMC-3) (KCCM11327P),Ashton Bauber Jonesny HKMC-4 (Acinetobacter johnsonii HKMC-4) (KCCM11328P),Bacillus Betanensis HKMC-5 (Bacillus vietnamensis HKMC-5) (KCCM11329P),Brevi Bacillus Invocatus HKMC-6 (Brevibacillus invocatus HKMC-6) (KCCM11330P),Deinokokkusu Peckus HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P),Reef Sonia Soli HKMC-8 (Leifsonia left HKMC-8) (KCCM11332P),Pseudomonas Nitroly Dysense HKMC-9 (Pseudomonas nitroreducens HKMC-9) (KCCM11333P) andSphingo Monas Aquatiles HKMC-10 (sphingomonas aquatilis HKMC-10) (KCCM11334P) andMethyl bromide Coma HKMC-11 (Methylobacterium komagatae HKMC-11) (KCCM11335P) on November 14, 2012 at the Korea Microorganism Conservation Center.

The object to which the biofilm of the present invention can be coated is a cooling device, and the cooling device includes a compressor, a blower, an eva core and the like. Especially, an object to which the biofilm of the present invention can be coated is EVA core. The air conditioner can be applied to any air conditioner of an air conditioner such as a home air conditioner or an automobile air conditioner. More preferably, it can be used in an EVA core of an air conditioner for an automobile.

The temperature of the indoor air is lowered by the cooling action of the EVA core, the humidity in the air is condensed and water is generated, and the moisture generated at this time, that is, the condensed water, falls out of the vehicle through the exhaust structure inside the EVA core , It is possible to control the odor removal when the air is introduced into the vehicle through the control of the odor microorganisms of the EVA core.

That is, breeding odorless microorganisms in advance so as to suppress the growth of odor microorganisms.

According to a further aspect of the invention there is provided a bacterium methyl Solarium AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) provides odorless EVA cores that do not cause odors in the air conditioner coated with biofilm.

In the present invention, a bacterium methyl Solarium In addition to aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) is coated with a biofilm, the microorganisms in addition to the methyl to coat the biofilm tumefaciens Beuraki Atum HKMC-2 (Methylobacterium brachiatum HKMC- 2) (KCCM11326P), a bacterium methyl Solarium Flash Tani HKMC-3 (Methylobacterium platani HKMC-3) (KCCM11327P) , Acinetobacter Jones you HKMC-4 (Acinetobacter johnsonii HKMC- 4) (KCCM11328P), Bacillus Betanensis HKMC-5 ( Bacillus vietnamensis HKMC-5) (KCCM11329P), Brevibacillus Inboard car tooth HKMC-6 (Brevibacillus invocatus HKMC- 6) (KCCM11330P), Day Noko kusu blood kusu HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P), Reef Sonia Soli HKMC-8 ( Leifsonia soli HKMC-8) (KCCM11332P), Pseudomonas nitro Lowry dew sense HKMC-9 (Pseudomonas nitroreducens HKMC- 9) (KCCM11333P) and Sphingomonas Aqua Antilles HKMC-10 (sphingomonas aquatilis HKMC-10) (KCCM11334P) and methyl tumefaciens The horseshoe HKMC-11 ( Methylobacterium The present invention can provide an odorless EVA core which does not cause odor in the air conditioner, characterized by additionally coating one or more microorganisms selected from the group consisting of komagatae HKMC-11 (KCCM11335P) The present invention is not limited to microorganisms, and all microorganisms capable of producing an odorless effect through a combination of various microorganisms are possible.

The material of the EVA core is aluminum or an aluminum alloy. EVA cores are manufactured using an EVA core that has been subjected to an antibacterial treatment or a non-antibacterial material. However, Eva core is not limited to aluminum or aluminum alloy. In general, Eva core can be used as a material for alloys such as copper (copper), which has good thermal conductivity and excellent corrosion resistance, A heat exchanger may be connected to the PELTIER device, and any material having a similar structure that facilitates heat exchange may be used as the material.

The methyl < RTI ID = 0.0 > AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) Biofilm inhibits the growth of microorganisms that cause odor and does not cause odor.

When the biofilm is coated on the surface of the EVA core, deposition and propagation of bacteria and fungi, which cause bad odors, are prevented. That is, when the biofilm is coated on the surface of the EVA core by using a specific microorganism or the like, other kinds of bacteria and fungi living in environments other than the specific microorganisms and the like can not be deposited and propagated on the EVA core surface.

Odorless biofilm is not limited to those consisting of only one kind of the microorganism to the methyl tumefaciens AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) This biofilm-coated EVA core was prepared by adding microbacterium Brckyatum HKMC-2 ( Methylobacterium brachiatum HKMC-2) (KCCM11326P) , a bacterium methyl Solarium Flash Tani HKMC-3 (Methylobacterium platani HKMC-3) (KCCM11327P) , Acinetobacter Jones you HKMC-4 (Acinetobacter johnsonii HKMC-4) (KCCM11328P), Bacillus Betanensis HKMC-5 ( Bacillus vietnamensis HKMC-5) (KCCM11329P), Brevibacillus Invoke Car Bluetooth HKMC-6 (Brevibacillus invocatus HKMC-6) (KCCM11330P) , Day Noko kusu blood kusu HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P), Reef Sonia Solid HKMC-8 (Leifsonia left HKMC-8) (KCCM11332P), Pseudomonas nitro Lowry dew sense HKMC-9 (Pseudomonas nitroreducens HKMC- 9) (KCCM11333P) and Sphingomonas Aqua subtilis HKMC-10 (sphingomonas aquatilis HKMC- 10) (KCCM11334P) and methyl tumefaciens The horseshoe HKMC-11 ( Methylobacterium (KCCM11335P). The biofilm, which is coated with one or more microorganisms selected from the group consisting of K.K.M.C., K.Magatae HKMC-11) (KCCM11335P), inhibits the growth of microorganisms causing odors, and does not cause odors.

According to another aspect of the present invention,

(i) On the surface of the EVA core , methylobacterium Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) applying the medium favorable to the propagation;

(ii) a methyl on the surface of the medium is applied tumefaciens Affixing the aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P); and

(iii) the methylobacterium AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) to form a biofilm;

The present invention provides an odorless EVA core manufacturing method that does not cause odor in the air conditioner including the above-described odor eliminating device.

In the step (i), the step of applying the culture medium favorable for breeding is a step that can be omitted if the microorganism can be naturally deposited as a means for facilitating the deposition of microorganisms.

In step (ii) above , methylobacterium In addition to aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) bacterium with methyl Solarium Beuraki Atum HKMC-2 (Methylobacterium brachiatum HKMC- 2) (KCCM11326P), a bacterium methyl Solarium Flash Tani HKMC-3 (Methylobacterium platani HKMC-3) (KCCM11327P) , Acinetobacter Jones you HKMC-4 (Acinetobacter johnsonii HKMC- 4) (KCCM11328P), Bacillus Betanensis HKMC-5 ( Bacillus vietnamensis HKMC-5) (KCCM11329P), Brevibacillus Inboard car tooth HKMC-6 (Brevibacillus invocatus HKMC- 6) (KCCM11330P), Day Noko kusu blood kusu HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P), Reef Sonia Solid HKMC-8 (Leifsonia soli HKMC- 8) (KCCM11332P), Pseudomonas nitro Lowry dew sense HKMC-9 (Pseudomonas nitroreducens HKMC- 9) (KCCM11333P) and Sphingomonas Aqua subtilis HKMC-10 (sphingomonas aquatilis HKMC- 10) (KCCM11334P) and methyl tumefaciens The horseshoe HKMC-11 ( Methylobacterium by coating with one or two or more microorganisms selected from the group consisting of komagatae HKMC-11) (KCCM11335P) Additionally biofilm can be prepared odorless biofilm through a combination of various odor microorganisms and growth of malodor microorganisms through which .

According to a preferred embodiment of the present invention, the surface of the EVA core may be made of aluminum or an aluminum alloy, and the aluminum or aluminum alloy may be coated with an antibacterial EVA core, Can be manufactured. Also Aluminum or an aluminum alloy can be produced by microbial inoculation without antimicrobial treatment.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings, in which an identification of an odorless microorganism in an eva core according to a preferred embodiment of the present invention and an odorless biofilm for an air conditioner using the same.

One. Example  1: Odor smell Secured used car

No. Car type sample type One Vehicle A Eva Core 2 Vehicle type B Eva Core 3 Vehicle type C Eva Core 4 Vehicle type D Eva Core 5 Vehicle E Eva Core

The present inventors secured five types of used cars that smelled bad odors and tried to sample the EVA core specimens by removing the EVA cores mounted on the vehicle types A to E, respectively.

2. Example  2 : Eva Core  Sample Sampling

The EVA core obtained from the used odors A to E was refrigerated at 4 ° C until the EVA core sample was used, and then sealed with a polyethylene bag. In order to isolate and cultivate the microorganisms, 5 g each of the fin samples was collected from each of the EVA cores using sterilized long nose pliers at arbitrary sites including the front part and the rear part.

3. Example  3: From Eva Core  Microbial Tally  process

The procedure and the method of desorbing microorganisms from the EVA core are described below.

① Mix the samples extracted from Eva core into a mixer.

② Add sterilized 1 × Phosphate buffed saline (PBS) to a 200 ml mixer.

③ Mix the mixed sample with PBS for 30 seconds.

④ Place mixer in ice for 1 minute.

⑤ Repeat steps ③-④ twice.

⑥ The suspension is centrifuged at 13000 rpm at 4 ° C for 3 minutes.

⑦ Take only the supernatant and transfer it to a new tube.

⑧ Wet the sterilized cotton swab with the supernatant and wipe the surface of the Eva core sample several times.

⑨ Put only the head in the supernatant liquid and polish the wiped swab.

⑩ Mix precipitant obtained in step ⑥ with mixture ⑨ and use it as stock solution.

The above processes (1) to (10) were physically removed from the EVA cores mounted on the vehicle A to the vehicle E to isolate the microorganisms.

4. Example  4: Isolation culture of bacteria

The separation of airborne germs is generally accomplished by heterotrophic culture of aerobic heterotrophic bacteria, commonly referred to as general germs. The PTYG agar medium and the R2A agar medium are used as the combined nutrient medium for the isolation of general bacteria. PTYG agar medium was prepared by mixing 0.25 g (Difco), 0.25 g (Difco), 0.2 g (Difco), 0.2 g of yeast extract, 0.1 g of Difco, 0.5 g of Difucor, 30 mg of MgSO ₄ , ₃ mg of CaCl ₂ (Difco) was added to 980 ml of distilled water, adjusted to pH 7.0, and autoclaved at 121 ° C for 15 minutes. R2A arar medium contains 0.5 g of Yeast extract (Difco), 0.5 g of Proteose peptone (Difco), 0.5 g of Casamino acids (Difco), 0.5 g of Dextrose (Difco), 0.5 g of Soluble starch (Difco), 0.3 g of sodium pyruvate (Difco), Dipotassium sulfate 0.3 g (Difco), Magnesium sulfate 0.05 g (Difco) and Bacto agar 15 g (Difco) were added to 980 ml of distilled water and adjusted to pH 7.2 and autoclaved at 121 ° C for 15 minutes. Three antibiotics were used for the isolation of common bacteria (Table 2). Antibiotic medium was prepared by inoculating each antibiotic at a concentration of 100 ppm after filter sterilization at a temperature of about 50 ° C.

No. Antibiotic line manufacturer One Kanamycin Aminoglycoside Sigma 2 Ampicillin beta-lactam Sigma 3 Chloramphenicol Chloramphenicol Sigma

5. Example  5: Isolation culture of fungus (mold)

Isolation cultures of air-conditioned fungi (fungi) are separated from aerobic plate culture in nutrient medium. Potato dextrose agar medium and Malt extract agar medium were used for the separation culture of fungi (fungi). Potato dextrose agar medium was prepared by placing Potato starch 4 g (Difco), Dextrose 20 g (Difco) and Bacto agar 15 g (Difco) in 980 ml of distilled water, adjusting the pH to 5.1, and autoclaving at 121 ° C for 15 minutes. Malt extract agar medium was prepared by adding Malt extract 20 g (Difco) and Bacto agar 15 g (Difco) to 980 ml of distilled water and adjusting the pH to 5.0, followed by high pressure sterilization at 121 ° C for 15 minutes.

A 90 mm × 15 mm petri dish was used for the separation of fungi. A petri dish of 60 mm × 15 mm was used for separate culture of cultured fungi.

6. Example  6: Eva Core  On the whole medium in which the microorganism has been cultured Dominance  Isolation culture of strain

In order to isolate and cultivate the dominant strains, first, various dominant strains should be selected through a morphological approach such as dilution ratio, colony color, size, and shape. Therefore, the dominant strains are separately cultured according to the following procedure.

Separation Separate fungi and bacteria from the culture medium.

② Inoculate various bacterial strains of different morphology into a complex medium using a loop and isolate them pure.

③ Select the best growth medium among the inoculated medium and subculture.

④ The mold is separated from the end of the hypha using scalpel and then inoculated into the complex medium.

⑤ Select the medium that has the best growth among the medium inoculated with the fungal strain and subculture.

7. Example  7: Eva Core Dominance  Analysis of genetic characteristics of bacteria

Fingerprints investigation through REP-PCR pattern analysis

REP-PCR is a molecular biologic method for analyzing the structure of bacterial chromosomes. It is a fingerprinting method that distinguishes each bacterial strain from other bacteria. To perform REP-PCR, genetic characteristics were analyzed according to the following procedures.

(One) Cell lysis  step

1) Transfer 2.5μl of Lyse-N-Go PCR Reagent (Thermo) into PCR tube.

② Pipet the colonies on a clean bench and pipet into the upper tubes. Be careful not to get enough amount of solution to pour.

③ Cultivate in PCR machine according to manufacturer's instructions.

(4) Repeat the cycle of the Lysis program described in Table 3 below, and leave it at 80 DEG C in 9 cycles without turning off.

Cycle Temperature (° C) Time (seconds) One 65 30 2 8 30 3 65 90 4 97 180 5 8 60 6 65 180 7 97 60 8 65 60 9 80 hold

(2) PCR reaction

The components required for the PCR reaction described in Table 4 below were mixed in the required amount to prepare a reaction mixture. The reaction mixture was subjected to pre-denaturation at 94 ° C for 7 minutes, denaturation step denaturation) denaturation at 92 ° C for 1 min, annealing at 51.5 ° C for 1 min, extension at 65 ° C for 8 min, denaturation, cooling and extension were performed 33 times to perform PCR amplification.

① dNTP (2.5 mM each) 12.5 μl ② Gitschier buffer 5.0 μl ③ DMSO (100%) 2.5 μl ④ Autoclaved 3 ^o DW 0.3 μl ⑤ BOXA1R primer (50 pmole / l) 1.0 μl 5'CTACGGCAAGGCGACGCTGACG ⑥ BSA (10 mg / ml) 0.4 μl ⑦ Bacterial DNA 2.5 μl ⑧ Taq polymerase (Roche) (5 U / mu l) 0.8 μl

step 1 93 ℃ 7min step 2 92 ° C 1 min step 3 51.5 DEG C 1 min step 4 65 ℃ 8min step 2,3,4: additional 33 cycles step 6 65 ℃ 16 min step 7 4 ℃

(3) Come 전공기

DNA fragments amplified by each PCR were taken and 1.2 - 1.5% agarose gel with EtBr was used. 6x dye was mixed with sample in 1: 5 ratio and as much as possible. Since most PCR products are between 100 and 1000 bp, a 100 bp ladder is loaded together and electrophoresed as slowly as possible (50 V) until the middle of the bromophenol blue and xylene cyanol dye reaches the middle of the entire gel. The same DNA pattern on the gel is regarded as the same strain.

(4) Air conditioners Dominance  16S of bacteria rRNA  Identification through gene analysis

The 16S rRNA (ribosomal ribonucleic acid) gene is used for the identification of bacterial genomic classification, and it is possible to identify bacteria classified at genus and species level by REP-PCR. 16S rRNA is an RNA that constitutes a ribosome by interacting with various proteins. Based on the genetic similarity of 16S rRNA, since the complete sequence or nucleotide sequence of the oligonucleotide list is revealed in more than 2000 bacteria, So that bacteria can be divided into several major groups. It is recognized that the degree of 16S rRNA sequence similarity reflects the phylogenetic distance between organisms because the rate of change of the 16S rRNA gene sequence is much smaller than that of other genomic sequences in most genomes. The method of identifying the microorganisms according to their similarity by analyzing the base sequence of the 16S rRNA gene fragment has been used as a typical identification method for identification of microorganisms, particularly industrially useful microorganisms, together with the above-described fatty acid analysis and carbohydrate magnetization assay.

<16S rRNA PCR>

PCR condtions (Total 50 쨉 l): The remaining solutions except for DNA and Taq were mixed in the required amount as shown in Table 6 below, and 44.5 쨉 l was added to the above lysis solution. Denaturation at 94 ° C for 5 minutes, denaturation at 94 ° C for 1 minute, annealing at 55 ° C for 1 minute, extension (extension) as described in Table 7, ) At 72 DEG C for 1 minute and 30 seconds, and denaturation, cooling and extension steps were performed 29 times to perform PCR amplification.

Autoclaved 3 ^o DW 22 쨉 l 10xbuffer (Roche) 5 μl dNTP (Roche, 2.5 mM) 5 μl DMSO 5 μl BSA (10 mg / ml) 2.5 μl 27 mf (20 pmole / l) 2.5 μl 1492 r (20 pmole / l) 2.5 μl DNA 5 μl Taq (Roche) 0.5 μl

step 1 94 ° C 5min step 2 94 ° C 1 min step 3 55 ° C 1 min step 4 72 ℃ 1min 30sec Go to step 2: additional 29 cycles step 6 72 ℃ 10 min step 7 4 ℃ hold

(5) PCR 정화

The products amplified by 16S-rRNA PCR were purified using the Qiaquick PCR purification kit according to the following procedure.

① Put 5 times PB buffer of PCR product.

② Divide the mixed solution in the QIAquick column.

③ Centrifuge for 1 minute to bind DNA and remove the mixed solution.

④ Add 750 μl of PE buffer to the QIAquick column for wash, centrifuge for 1 minute, and remove the mixed solution.

⑤ Centrifuge again for 1 minute.

⑥ Move the QIAquick column to a new tube.

⑦ Put 30 μl of EB buffer for 1 min to extract DNA.

⑧ Centrifuge for 1 minute to collect the DNA dissolved in EB in the tube.

(6) Name of each microorganism separated and characteristics of microorganism

<Microorganism 1>

1. Name of microorganism: HKMC-1

Genus: Methylobacterium

Species: aquaticum

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 1

<Microorganism 2>

1. Name of microorganism: HKMC-2

Genus: Methylobacterium

Species: brachiatum

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 2

<Microorganism 3>

1. Name of microorganism: HKMC-3

Genus: Methylobacterium

Species: platani

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 3

<Microorganism 4>

1. Name of microorganism: HKMC-4

Genus: Acinetobacter

Species: johnsonii

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 4

<Microorganism 5>

1. Name of microorganism: HKMC-5

Name: Bacillus

Species: vietnamensis

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 5

<Microorganism 6>

1. Name of microorganism: HKMC-6

Brevibacillus

Species: invocatus

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 6

<Microorganism 7>

1. Name of microorganism: HKMC-7

Genus: Deinococcus

Species: ficus

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 7

<Microorganism 8>

1. Name of microorganism: HKMC-8

Genus: Leifsonia

Species: soli

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 8

<Microorganism 9>

1. Name of microorganism: HKMC-9

Genus: Pseudomonas

Species: nitroreducens

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 9

<Microorganism 10>

1. Name of microorganism: HKMC-10

Genus: Sphingomonas

Species: aquatilis

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 10

<Microorganism 11>

1. Name of microorganism: HKMC-11

Genus: Methylobacterium

Species: komagatae

2. Restoration conditions

   end. Restorer

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

   I. Temperature (° C) Culture at 28 ° C for 7 days

3. Badge

(1) Composition: PTYG medium (0.25 g Peptone per liter, 0.25 g Tryptone, 0.5 g Yeast extract, 0.5 g Glucose, 30 mg MgSO ₄ , 3 mg CaCl ₂ ) or R2A medium.

    (2) pH: 7.0

    (3) Sterilization conditions: 121 占 폚 for 20 minutes

4. Culture conditions

   end. Aerobic, anaerobic: aerobic

   I. Temperature (℃) 28 ℃

   All. Shaking, politic (liquid, solid) can be used without distinction

5. Conservation conditions

    Temperature (° C) -70 ° C

6. 16S rRNA gene sequence

SEQ ID NO: 11

8. Example  8: Sensory evaluation of isolated microorganisms

(One) In nutrient media  Sensory evaluation

For the analysis of the sensory characteristics of the microorganisms identified from Example 7 above, the microorganisms were cultured in a nutrient medium from which the microorganisms were isolated for 7 days at 28 ° C. The following describes the process of culturing bacteria in a nutrient medium.

① Pure separation Separate the cultured microorganisms into liquid nutrient medium.

② The inoculated medium is incubated at 28 ℃ for 5-7 days.

③ Inoculate the solid nutrient medium with 100 ㎕ of the cultured cells in the liquid medium.

④ Spread the inoculated cells evenly using a spreader.

⑤ Seal the Petri dishes and incubate at 28 ℃ for 10 days.

(2) Sensory evaluation in an aluminum pin culture medium

Square size aluminum fins were sterilized and dipped into dust and nutrient media. Thereafter, bacteria were inoculated and cultured in the same manner as described in step (2) - (4) in the nutrient medium, and then evaluated. The results of sensory evaluation were shown in Table 8 below.

① Aluminum pin treated with antimicrobial agent: It is a coating of EVA core finished product which can be purchased on the market which is mass produced with antimicrobial coating on aluminum which is the main material of EVA core.

② Aluminum Fins that have not been treated with antimicrobial agent and have only hydrophilic coating: It is an aluminum pin that has undergone a hydrophilic process only during the coating process of EVA core. Generally, the hydrophilic process and the antibacterial process are performed at the same time. It is specially designed to compare antimicrobial coated pins with non-antibacterial coated pins. Eva core is made of aluminum pin for light weight but it can be made of various metal such as copper material.

NO. Antimicrobial agent pin Antimicrobial agent pin 16S rRNA One Odorless Odorless Methylobacterium aquaticum HKMC -One 2 Odorless Odorless Methylobacterium brachiatum HKMC-2 3 Odorless Odorless Methylobacterium platani HKMC-3 4 Odorless Odorless Acinetobacter johnsonii HKMC-4 5 Odorless Odorless Bacillus vietnamensis HKMC-5 6 Odorless Odorless Brevibacillus invocatus HKMC-6 7 Odorless Odorless Deinococcus ficus HKMC-7 8 Odorless Odorless Leifsonia soli HKMC-8 9 Odorless Odorless Pseudomonas nitroreducens HKMC-9 10 Odorless Odorless Sphingomonas aquatilis HKMC-10 11 Odorless Odorless Methylobacterium komagatae HKMC-11

All of the 11 kinds of microorganisms were inoculated with the aluminum antimicrobial agent fins and the antimicrobial agent fungus, and the results were all odorless. The application field of the above-mentioned odorless microorganisms can be sufficiently used for the purpose of preventing bad odors caused by microorganisms inhabiting the EVA core of the air conditioner regardless of the metal material.

Korea Microorganism Conservation Center (overseas) KCCM11325P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11326P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11327P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11328P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11329P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11330P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11331P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11332P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11333P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11334P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11335P 20121114

<110> Hyundai Motor Company <120> Methylobacterium brachiatum for Odorless Bioflim-Coated          Evaporator Core and uses thereof <160> 11 <170> Kopatentin 1.71 <210> 1 <211> 1411 <212> DNA <213> Methylobacterium aquaticum HKMC-1 <220> <221> rRNA &Lt; 222 > (1) <223> 16S rRNA <400> 1 cagagcgaac gctggcggca ggcttaacac atgcaagtcg agcgggccct tcggggtcag 60 cggcagacgg gtgagtaacg cgtgggaacg tgcccttcgg ttcggaataa ctcagggaaa 120 cttgagctaa taccggatac gcccttatgg ggaaaggctt gactgccgaa ggatcggccc 180 gcgtctgatt agctagttgg tgaggttacg gctcaccaag gcgacgatca gtagctggtc 240 tgagaggatg atcagccaca ctgggactga gacacggccc agactcctac gggaggcagc 300 agtggggaat attggacaat gggggcaacc ctgatccagc catgccgcgt gagtgatgac 360 ggccttaggg ttgtaaagct ctttctccgg gacgataatg acggtaccgg aggaataagc 420 cccggctaac ttcgtgccag cagccgcggt aatacgaagg gggctagcgt tgctcggaat 480 cactgggcgt aaagggcgcg taggcggctg atttagtcga gggtgaaagc ccgtggctca 540 accacggaat ggccttcgat actggttggc ttgagaccgg aagaggacag cggaactgcg 600 agtgtagagg tgaaattcgt agatattcgc aagaacacca gtggcgaagg cggctgtctg 660 gtccggttct gacgctgagg cgcgaaagcg tggggagcaa acaggattag ataccctggt 720 agtccacgct gtaaacgatg aatgctagcc gttggggtgc atgcacctca gtggcgccgc 780 taacgcatta agcattccgc ctggggagta cggtcgcaag attaaaactc aaaggaattg 840 acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gcagaacctt 900 accatccctt gacatggcgt gttatccgga gagatccggg gtccccttcg ggggcgcgca 960 cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa 1020 cgagcgcaac ccacgtccct agttgccatc atttggttgg gcactctggg gagactgccg 1080 gtgataagcc gcgaggaagg tgtggatgac gtcaagtcct catggccctt acgggatggg 1140 ctacacacgt gctacaatgg cggtgacaat gggcagcgaa ggggcgacct ggagcgaatc 1200 cccaaaagcc gtctcagttc ggattgcact ctgcaactcg ggtgcatgaa ggcggaatcg 1260 ctagtaatcg tggatcagca cgccacggtg aatacgttcc cgggccttgt acacaccgcc 1320 cgtcacacca tgggagttgg tcttacccga cggcgctgcg ccaaccgcaa ggaggcaggc 1380 gaccacggta gggtcagcga ctggggtgaa g 1411 <210> 2 <211> 1416 <212> DNA <213> Methylobacterium brachiatum HKMC-2 <220> <221> rRNA &Lt; 222 > (1) .. (1416) <223> 16S rRNA <400> 2 ggctgagaga gaacgggggg ggcaggttta acacatacaa gtagagggga cctttggggg 60 tcagcggcgg acgggtgagt aacgcgtggg aacgtgccct ctggttcgga ataactcagg 120 gaaacttgag ctaataccgg atacgccctt ttggggaaag gcttgctgcc ggaggatcgg 180 cccgcgtctg attagctagt tggtgaggta acggctcacc aaggcgacga tcagtagctg 240 gtctgagagg atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtgggg aatattggac aatgggcgca agcctgatcc agccatgccg cgtgagtgat 360 gaaggcctta gggttgtaaa gctcttttat ccgggacgat aatgacggta ccggaggaat 420 aagccccggc taacttcgtg ccagcagccg cggtaatacg aagggggcta gcgttgctcg 480 gaatcactgg gcgtaaaggg cgcgtaggcg gcgttttaag tcgggggtga aagcctgtgg 540 ctcaaccaca gaatggcctt cgatactggg acgcttgagt atggtagagg ttggtggaac 600 tgcgagtgta gaggtgaaat tcgtagatat tcgcaagaac accggtggcg aaggcggcca 660 actggaccat tactgacgct gaggcgcgaa agcgtgggga gcaaacagga ttagataccc 720 tggtagtcca cgccgtaaac gatgaatgcc agctgttggg gtgcttgcac ctcagtagcg 780 cagctaacgc tttgagcatt ccgcctgggg agtacggtcg caagattaaa actcaaagga 840 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgcagaa 900 ccttaccatc ctttgacatg gcgtgttatg gggagagatt cccagtcctc ttcggaggcg 960 cgcacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1020 gcaacgagcg caacccacgt ccttagttgc catcattcag ttgggcactc tagggagact 1080 gccggtgata agccgcgagg aaggtgtgga tgacgtcaag tcctcatggc ccttacggga 1140 tgggctacac acgtgctaca atggcggtga cagtgggacg cgaaggggcg acctggagca 1200 aatccccaaa agccgtctca gttcggattg cactctgcaa ctcgggtgca tgaaggcgga 1260 atcgctagta atcgtggatc agcatgccac ggtgaatacg ttcccgggcc ttgtacacac 1320 cgcccgtcac accatgggag ttggtcttac ccgacggcgc tgcgccaacc gcaaggaggc 1380 aggcgaccac ggtagggtca gcgactgggg tgaagt 1416 <210> 3 <211> 1411 <212> DNA <213> Methylobacterium platani HKMC-3 <220> <221> rRNA &Lt; 222 > (1) <223> 16S rRNA <400> 3 gaacgaaggc ggcaggctta acacatgcaa gtagagggg catccctgtg gggttaacgg 60 cagacgggtg agtaaagggt gggaaagtgc cctttggttt ggaataaatt agggaaaact 120 gaagtaattc cggatacgtc cgagagggga aaggtttatc tgccgaagga tcggcccgcg 180 tctgattagc tagttggtga ggttatggct caccaaggcg acgatcagta gctggtctga 240 gaggatgatc agccacactg ggactgagac acggcccaga ctcctacggg aggcagcagt 300 ggggaatatt ggacaatggg ggcaaccctg atccagccat gccgcgtgag tgatgacggc 360 cttagggttg taaagctctt ttctccggga cgataatgac ggtaccggag gaataagccc 420 cggctaactt cgtgccagca gccgcggtaa tacgaagggg gctagcgttg ctcggaatca 480 ctgggcgtaa agggcgcgta ggcggctgat ttagtcgagg gtgaaagccc gtggctcaac 540 cacggaatgg ccttcgatac tgattggctt gagaccggaa gaggacagcg gaactgcgag 600 tgtagaggtg aaattcgtag atattcgcaa gaacaccagt ggcgaaggcg gctgtctggt 660 ccggttctga cgctgaggcg cgaaagcgtg gggagcaaac aggattagat accctggtag 720 tccacgctgt aaacgatgaa tgctagccgt tggggtgcat gcacctcagt ggcgccgcta 780 acgctttaag cattccgcct ggggagtacg gtcgcaagat taaaactcaa aggaattgac 840 gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcgc agaaccttac 900 catcccttga catggcatgc gagccggaga gatccggtgt tcccttcggg gacgtgcaca 960 caggtgctgc atggctgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1020 agcgcaaccc acgtcctcag ttgccatcat tcagttgggc actctgggga gactgccggt 1080 gataagccgc gaggaaggtg tggatgacgt caagtcctca tggcccttac gggatgggct 1140 acacacgtgc tacaatggcg gtgacaatgg gcagcgaagg ggcgacctgg agcgaatccc 1200 caaaagccgt ctcagttcgg attgcactct gcaactcggg tgcatgaagg cggaatcgct 1260 agtaatcgtg gatcagcatg ccacggtgaa tacgttcccg ggccttgtac acaccgcccg 1320 tcacaccatg ggagttggtc ttacccgacg gcgctgcgcc aaccgcaagg aggcaggcga 1380 ccacggtagg gtcagcgact ggggtgaagt c 1411 <210> 4 <211> 1472 <212> DNA <213> Acinetobacter johnsonii HKMC-4 <220> <221> rRNA &Lt; 222 > (1) .. (1472) <223> 16S rRNA <400> 4 ggctcagatt gaacgctggc ggcaggctta acacatgcaa gtcgagcggg gaaaggtagc 60 ttgctaccta acctagcggc ggacgggtga gtaatgctta ggaatctgcc tattagtggg 120 ggacaacatt ccgaaaggaa tgctaatacc gcatacgccc tacgggggaa agcaggggat 180 cttcggacct tgcgctaata gatgagccta agtcagatta gctagttggt ggggtaaagg 240 cctaccaagg cgacgatctg tagcgggtct gagaggatga tccgccacac tgggactgag 300 acacggccca gactcctacg ggaggcagca gtggggaata ttggacaatg ggcgaaagcc 360 tgatccagcc atgccgcgtg tgtgaagaag gccttttggt tgtaaagcac tttaagcgag 420 gaggaggcta cttagattaa tactctagga tagtggacgt tactcgcaga ataagcaccg 480 gctaactctg tgccagcagc cgcggtaata cagagggtgc gagcgttaat cggatttact 540 gggcgtaaag cgtgcgtagg cggcttctta agtcggatgt gaaatccctg agcttaactt 600 aggaattgca ttcgatactg ggaagctaga gtatgggaga ggatggtaga attccaggtg 660 tagcggtgaa atgcgtagag atctggagga ataccgatgg cgaaggcagc catctggcct 720 aatactgacg ctgaggtacg aaagcatggg gagcaaacag gattagatac cctggtagtc 780 catgccgtaa acgatgtcta ctagccgttg gggcctttga ggctttagtg gcgcagctaa 840 cgcgataagt agaccgcctg gggagtacgg tcgcaagact aaaactcaaa tgaattgacg 900 ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc 960 tggtcttgac atagtaagaa ctttccagag atggattggt gccttcggga acttacatac 1020 aggtgctgca tggctgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga 1080 gcgcaaccct tttccttatt tgccagcggg ttaagccggg aactttaagg atactgccag 1140 tgacaaactg gaggaaggcg gggacgacgt caagtcatca tggcccttac gaccagggct 1200 acacacgtgc tacaatggtc ggtacaaagg gttgctacct agcgatagga tgctaatctc 1260 aaaaagccga tcgtagtccg gattggagtc tgcaactcga ctccatgaag tcggaatcgc 1320 tagtaatcgc ggatcagaat gccgcggtga atacgttccc gggccttgta cacaccgccc 1380 gtcacaccat gggaatttgt tgcaccagaa gtaggtagtc taaccgcaag gaggacgctt 1440 accacggtgt ggccgatgac tggggtgaag tc 1472 <210> 5 <211> 1485 <212> DNA <213> Bacillus vietnamensis HKMC-5 <220> <221> rRNA &Lt; 222 > (1) .. (1485) <223> 16S rRNA <400> 5 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtcgagcgga ttgatgggag 60 cttgctccct gatatcagcg gcggacgggt gagtaacacg tgggtaacct gcctgtaaga 120 ctgggataac tccgggaaac cggggctaat accggataac tcatttcctc gcatgaggaa 180 atgttgaaag atggcttcgg ctatcactta cagatggacc cgcggcgcat tagctagttg 240 gtgaggtaac ggctcaccaa ggcaacgatg cgtagccgac ctgagagggt gatcggccac 300 actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttccgcaa 360 tggacgaaag tctgacggag caacgccgcg tgagtgatga aggttttcgg atcgtaaagc 420 tctgttgtta gggaagaaca agtgccgttc gaatagggcg gcacattgac ggtacctaac 480 cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg 540 tccggaatta ttgggcgtaa agcgcgcgca ggtggttcct taagtctgat gtgaaagccc 600 acggctcaac cgtggagggt cattggaaac tggggaactt gagtgcagaa gaggaaagtg 660 gaattccaag tgtagcggtg aaatgcgtag atatttggag gaacaccagt ggcgaaggcg 720 actttctggt ctgtaactga cactgaggcg cgaaagcgtg gggagcaaac aggattagat 780 accctggtag tccacgccgt aaacgatgag tgctaagtgt tagggggttt ccgcccctta 840 gtgctgcagc taacgcatta agcactccgc ctggggagta cggtcgcaag actgaaactc 900 aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc 960 gaagaacctt accaggtctt gacatcctct gacaacccta gagatagggc tttccccttc 1020 gggggacaga gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 1080 taagtcccgc aacgagcgca acccttgatc ttagttgcca gcattcagtt gggcactcta 1140 agatgactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1200 tatgacctgg gctacacacg tgctacaatg gacggtacaa agggcagcga gaccgcgagg 1260 tttagccaat cccataaaac cgttctcagt tcggattgta ggctgcaact cgcctacatg 1320 aagctggaat cgctagtaat cgcggatcag catgccgcgg tgaatacgtt cccgggcctt 1380 gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt 1440 ttggagccag ccgcctaagg tgggacagat gattggggtg aagtc 1485 <210> 6 <211> 1470 <212> DNA <213> Brevibacillus invocatus HKMC-6 <220> <221> rRNA &Lt; 222 > (1) .. (1470) <223> 16S rRNA <400> 6 ggttcaggac gaacccgggg ggcgtgctta atccatgcaa gtcgagggag ttttttcaga 60 ccctagcggc ggacgggtga gtaacacgta ggcaacctgc ccataagact gggataacat 120 agggaaactt atgctaatac cggatagagt cttctcccgc atgagagaag acggaaaggt 180 ggcgcaagct accacttgtg gatgggcctg cggcgcatta gctagttggt ggggtaacgg 240 cctaccaagg cgacgatgcg tagccgacct gagagggtga ccggccacac tgggactgag 300 acacggccca gactcctacg ggaggcagca gtagggaatt ttccacaatg gacgaaagtc 360 tgatggagca acgccgcgtg aacgatgaag gccttcgggt tgtaaagttc tgttgtcagg 420 gacgaacaag taccgttcga atagggcggt accttgacgg tacctgacga gaaagccacg 480 gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt 540 gggcgtaaag cgcgcgcagg cggctatgta agtctggtgt taaagcccgg ggctcaaccc 600 cggttcgcat cggaaactgt gtagcttgag tgcagaagag gaaagcggta ttccacgtgt 660 agcggtgaaa tgcgtagaga tgtggaggaa caccagtggc gaaggcggct ttttggtctg 720 taactgacgc tgaggcgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc 780 acgccgtaaa cgatgagtgc taggtgttag gggtttcaat acccttagtg ccgcagctaa 840 cgcaataagc actccgcctg gggagtacgg tcgcaagact gaaactcaaa ggaattgacg 900 ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc 960 aggtcttgac atcccgctga ccgctctgga gacagagctt cccttcgggg cagcggtgac 1020 aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga 1080 gcgcaaccct tatctttagt tgccagcatt cagttgggca ctctagagag actgccgtcg 1140 acaagacgga ggaaggcggg gatgacgtca aatcatcatg ccccttatga cctgggctac 1200 acacgtgcta caatggttgg tacaacggga tgctacctcg cgagaggatg ccaatctctt 1260 aaaaccaatc tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta 1320 gtaatcgcgg atcagcatgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt 1380 cacaccacgg gagtttgcaa cacccgaagt cggtgaggta accgcaagga gccagccgcc 1440 gaaggtgggg tagatgactg gggtgaagtc 1470 <210> 7 <211> 1442 <212> DNA <213> Deinococcus ficus HKMC-7 <220> <221> rRNA &Lt; 222 > (1) .. (1442) <223> 16S rRNA <400> 7 ggctcagggt gaacgctggc ggcgtgctta agacatgcaa gtcgaacgca gtcttcggac 60 tgagtggcgc acgggtgagt aacacgtaac tgacctgccc caaagtcgcg gataactggc 120 cgaaaggtca gctaatacgt gatgtgatgt cagattctgt tctgccatta aaggtttact 180 gctttgggat ggggttgcgt tccatcagct agatggtgag gtaaaggctc accatggcga 240 cgacggatag ccggcctgag agggtggccg gccacagggg cactgagaca cgggtcccac 300 tcctacggga ggcagcagtt aggaatcttc cccaatgggc gcaagcctga gggagcgacg 360 ccgcgtgagg gatgacggtc ctcggattgt aaacctctga acccacgacg aaaggccccg 420 tatggggaga tgacggtagt ggggtaatag caccggctaa ctccgtgcca gcagccgcgg 480 taatacggag ggtgcaagcg ttacccggaa tcactgggcg taaagggcgt gtaggcggaa 540 atctaagtct ggttttaaag accgaagctc aacttcggaa gtggactgga tactggattt 600 ctagacctct ggagaggaaa ccggaattcc tggtgtagcg gtggaatgcg tagataccag 660 gggaacacc gatggcgaag gcaggtttct ggacagaagg tgacgctgag gcgcgaaagt 720 gtggggagcg aaccggatta gatacccggg tagtccacac cctaaacgat gtacgttggc 780 ctatggcagg atgctgtcat gggcgaagct aacgcgataa acgtaccgcc tgggaagtac 840 ggccgcaagg ttgaaactca aaggaattga cgggggcccg cacaagcggt ggagcatgtg 900 gtttaattcg aagcaacgcg aagaacctta ccaggtcttg acatcccaag aacctcccag 960 agatgggagg gtgcccctcg gggaacttgg agacaggtgc tgcatggctg tcgtcagctc 1020 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttgcctt tagttgccag 1080 cacttcgggt gggcactcta gagggactgc cggtgaaagc cggaggaagg cggggatgac 1140 gtctagtcag catggtcctt acgacctggg cgacacacgt gctacaatgg atggtacaac 1200 gcgcagccag cccgcgaggg tgagcgaatc gctgaaagcc atccccagtt cagatcggag 1260 tctgcaactc gactccgtga agttggaatc gctagtaatc gcgggtcagc ataccgcggt 1320 gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtac gttgcagttg 1380 aaaccgccgg gagccgcaag gcaggcgtct agactgtggc gcatgactgg ggtgaagtcg 1440 ta 1442 <210> 8 <211> 1455 <212> DNA <213> Leifsonia soli HKMC-8 <220> <221> rRNA &Lt; 222 > (1) .. (1455) <223> 16S rRNA <400> 8 tggctcagga cgaacgctgg cggcgtgctt aacacatgca agtcgaacga tgaacctgga 60 gcttgctccg ggggattagt ggcgaacggg tgagtaacac gtgagtaacc tgcccttgac 120 tctgggataa cctccggaaa cggaagctaa taccggatat gacgtacgga ggcatctcct 180 gtacgtggaa agaatttcgg tcaaggatgg actcgcggcc tatcaggtag ttggtgaggt 240 aacggcccac caagcctacg acgggtagcc ggcctgagag ggtgaccggc cacactggga 300 ctgagacacg gcccagactc ctacgggagg cagcagtggg gaatattgca caatgggcgc 360 aagcctgatg cagcaacgcc gcgtgaggga tgacggcctt cgggttgtaa acctctttta 420 gtagggaaga agcgaaagtg acggtacctg cagaaaaagc accggctaac tacgtgccag 480 cagccgcggt aatacgtagg gtgcgagcgt tgtccggaat tattgggcgt aaagagctcg 540 taggcggtct gtcgcgtctg ctgtgaaaac ccgaggctca acctcgggcc tgcagtgggt 600 acgggcagac tagagtgcgg taggggagaa tggaattcct ggtgtagcgg tggaatgcgc 660 agatatcagg aggaacaccg atggcgaagg cagttctctg ggccgtaact gacgctgagg 720 agcgaaagcg tggggagcga acaggattag ataccctggt agtccacgcc gtaaacgttg 780 ggcgctagat gtggggacca ttccacggtt tccgtgtcgc agctaacgca ttaagcgccc 840 cgcctgggga gtacggccgc aaggctaaaa ctcaaaggaa ttgacggggg cccgcacaag 900 cggcggagca tgcggattaa ttcgatgcaa cgcgaagaac cttaccaagg cttgacatat 960 acgagaacgg gccagaaatg gtcaactctt tggacactcg taaacaggtg gtgcatggtt 1020 gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca accctcgttc 1080 tatgttgcca gcgcgtaatg gcgggaactc ataggagact gccggggtca actcggagga 1140 aggtggggat gacgtcaaat catcatgccc cttatgtctt gggcttcacg catgctacaa 1200 tggccggtac aaagggctgc aataccgtaa ggtggagcga atcccaaaaa gccggtctca 1260 gttcggattg aggtctgcaa ctcgacctca tgaagtcgga gtcgctagta atcgcagatc 1320 agcaacgctg cggagaatac ctacccgggc cttgtacaca ccgcccgtca agtcatgaaa 1380 gtcggtaaca cccgaagcca ttggcccaac ccttgtggag ggagctgtcc aaggtgggat 1440 cggtgattac cagtt 1455 <210> 9 <211> 1471 <212> DNA <213> Pseudomonas nitroreducens HKMC-9 <220> <221> rRNA &Lt; 222 > (1) .. (1471) <223> 16S rRNA <400> 9 ggctcagatt gaacgctggc ggcaggccta acacatgcaa gtcgagcgga tgagtggagc 60 ttgctccatg attcagcggc ggacgggtga gtaatgccta ggaatctgcc tggtagtggg 120 ggacaacgtt tcgaaaggaa cgctaatacc gcatacgtcc tacgggagaa agcaggggac 180 cttcgggcct tgcgctatca gatgagccta ggtcggatta gctagttggt ggggtaaagg 240 cctaccaagg cgacgatccg taactggtct gagaggatga tcagtcacac tggaactgag 300 acacggtcca gactcctacg ggaggcagca gtggggaata ttggacaatg ggcgaaagcc 360 tgatccagcc atgccgcgtg tgtgaagaag gtcttcggat tgtaaagcac tttaagttgg 420 gaggaagggc agtaagttaa taccttgctg ttttgacgtt accaacagaa taagcaccgg 480 ctaacttcgt gccagcagcc gcggtaatac gaagggtgca agcgttaatc ggaattactg 540 ggcgtaaagc gcgcgtaggt ggtttggtaa gatggatgtg aaatccccgg gctcaacctg 600 ggaactgcat ccataactgc ctgactagag tacggtagag ggtggtggaa tttcctgtgt 660 agcggtgaaa tgcgtagata taggaaggaa caccagtggc gaaggcgacc acctggactg 720 atactgacac tgaggtgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc 780 acgccgtaaa cgatgtcgac tagccgttgg gatccttgag atcttagtgg cgcagctaac 840 gcgataagtc gaccgcctgg ggagtacggc cgcaaggtta aaactcaaat gaattgacgg 900 gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacct 960 ggccttgaca tgtccggaac cttgcagaga tgcgagggtg ccttcgggaa tcggaacaca 1020 ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgtaacgag 1080 cgcaaccctt gtccttagtt accagcacgt tatggtgggc actctaagga gactgccggt 1140 gacaaaccgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg gccagggcta 1200 cacacgtgct acaatggtcg gtacagaggg ttgccaagcc gcgaggtgga gctaatccca 1260 taaaaccgat cgtagtccgg atcgcagtct gcaactcgac tgcgtgaagt cggaatcgct 1320 agtaatcgtg aatcagaatg tcacggtgaa tacgttcccg ggccttgtac acaccgcccg 1380 tcacaccatg ggagtgggtt gctccagaag tagctagtct aaccgcaagg gggacggtta 1440 ccacggagtg attcatgact ggggtgaagt c 1471 <210> 10 <211> 1421 <212> DNA <213> Sphingomonas aquatilis HKMC-10 <220> <221> rRNA &Lt; 222 > (1) .. (1421) <223> 16S rRNA <400> 10 ggctcagaac gaacgctggc ggcatgccta acacatgcaa gtcgaacgag atcttcgggt 60 ctagtggcgc acgggtgcgt aacgcgtggg aatctgccct ttggttcgga ataacagttg 120 gaaacgactg ctaataccgg atgatgacga aagtccaaag atttatcgcc agaggatgag 180 cccgcgtagg attagctagt tggtgtggta aaggcgcacc aaggcgacga tccttagctg 240 gtctgagagg atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtgggg aatattggac aatgggcgaa agcctgatcc agcaatgccg cgtgagtgat 360 gaaggcctta gggttgtaaa gctcttttac ccgggatgat aatgacagta ccgggagaat 420 aagctccggc taactccgtg ccagcagccg cggtaatacg gagggagcta gcgttgttcg 480 gaattactgg gcgtaaagcg cacgtaggcg gctttgtaag ttagaggtga aagcctggag 540 ctcaactcca gaactgcctt taagactgca tcgcttgaat ccaggagagg tgagtggaat 600 tccgagtgta gaggtgaaat tcgtagatat tcggaagaac accagtggcg aaggcggctc 660 actggactgg tattgacgct gaggtgcgaa agcgtgggga gcaaacagga ttagataccc 720 tggtagtcca cgccgtaaac gatgataact agctgtccgg ggacttggtc tttgggtggc 780 gcagctaacg cattaagtta tccgcctggg gagtacggcc gcaaggttaa aactcaaatg 840 aattgacggg ggcctgcaca agcggtggag catgtggttt aattcgaagc aacgcgcaga 900 accttaccag cgtttgacat gtccggacga tttccagaga tggatctctt cccttcgggg 960 actggaacac aggtgctgca tggctgtcgt cagctcgtgt cgtgagatgt tgggttaagt 1020 cccgcaacga gcgcaaccct cgcctttagt taccatcatt tagttgggga ctctaaagga 1080 accgccggtg ataagccgga ggaaggtggg gatgacgtca agtcctcatg gcccttacgc 1140 gctgggctac acacgtgcta caatggcggt gacagtgggc agcaaactcg cgagagtgcg 1200 ctaatctcca aaagccgtct cagttcggat tgttctctgc aactcgagag catgaaggcg 1260 gaatcgctag taatcgcgga tcagcatgcc gcggtgaata cgttcccagg ccttgtacac 1320 accgcccgtc acaccatggg agttgggttc acccgaaggc gttgcgctaa ctcgcaagag 1380 aggcaggcga ccacggtggg cttagcgact ggggtgaagt c 1421 <210> 11 <211> 1419 <212> DNA &Lt; 213 > Methylobacterium komagatae HKMC-11 <220> <221> rRNA &Lt; 222 > (1) .. (1419) <223> 16S rRNA <400> 11 ggctcagagc gaacgctggc ggcaggctta acacatgcaa gtcgagcggg cctttcgggg 60 tcagcggcgg acgggtgagt aacgcgtggg aacgtgcctt ctggttcgga ataactcagg 120 gaaacttgag ctaataccgg atacgccctt ttggggaaag gtttactgcc ggaagatcgg 180 cccgcgtctg attagctagt tggtggggta acggcctacc aaggcgacga tcagtagctg 240 gtctgagagg atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtgggg aatattggac aatgggcgca agcctgatcc agccatgccg cgtgagtgat 360 gaaggcctta gggttgtaaa gctcttttat ccgggacgat aatgacggta ccggaggaat 420 aagccccggc taacttcgtg ccagcagccg cggtaatacg aagggggcta gcgttgctcg 480 gaatcactgg gcgtaaaggg cgcgtaggcg gtcttttaag tcgggggtga aagcctgtgg 540 ctcaaccaca gaattgcctt cgatactggg agacttgagt tcggaagagg ttggtggaac 600 tgcgagtgta gaggtgaaat tcgtagatat tcgcaagaac accggtggcg aaggcggcca 660 actggtccga cactgacgct gaggcgcgaa agcgtgggga gcaaacagga ttagataccc 720 tggtagtcca cgccgtaaac gatgaatgcc agccgttggg gggcttgcct ctcagtggcg 780 cagctaacgc tttgagcatt ccgcctgggg agtacggtcg caagattaaa actcaaagga 840 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgcagaa 900 ccttaccatc ctttgacatg gcgtgttacc cagagagatt tggggtccac ttcggtggcg 960 cgcacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1020 gcaacgagcg caacccacgt cctcagttgc catcattgag ttgggcactc tggggagact 1080 gccggtgata agccgcgagg aaggtgtgga tgacgtcaag tcctcatggc ccttacggga 1140 tgggctacac acgtgctaca atggcggtga cagtgggaag cgaacccgcg aggggaagca 1200 aatctccaaa agccgtctca gttcggattg cactctgcaa ctcgagtgca tgaaggcgga 1260 atcgctagta atcgtggatc agcatgccac ggtgaatacg ttcccgggcc ttgtacacac 1320 cgcccgtcac accatgggag ttggtcttac ccgacggcgc tgcgccgacc cgcgagggga 1380 gcaggcgacc acggtagggt cagcgactgg ggtgaagtc 1419

Claims (17)

delete delete delete delete Methyl tumefaciens aqua tikum (Methylobacterium aquaticum) of the air conditioner in odor odorless EVA core does not lead to coatings with biofilm formed by the propagation tumefaciens strain in aerobic conditions to Aqua tikum odorless methyl.
The method of claim 5, wherein in the microorganism methyl tumefaciens aqua tikum (Methylobacterium aquaticum) the growth by the EVA core coated with a formed biofilm is tumefaciens beuraki Atum (Methylobacterium brachiatum) methyl addition to the microorganism, bacterium methyl Solarium flash Tani (Methylobacterium platani), Acinetobacter Jones Needle (Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis), Breda ratio Bacillus inboard car tooth (Brevibacillus invocatus), Day Noko kusu blood Syracuse (Deinococcus ficus), grapefruits Sonia Solid (Leifsonia soli), Pseudomonas nitro Lowry dew sense (Pseudomonas nitroreducens), Sphingomonas aqua subtilis (sphingomonas aquatilis) and methyl tumefaciens coma have additionally one or microorganism or more kinds selected from the group consisting of a condition (Methylobacterium komagatae) In the air conditioning apparatus Odorless EVA core does not cause the birds.
6. The EVA core according to claim 5, wherein the EVA core is made of aluminum, an aluminum alloy, copper, or a copper alloy.
delete delete (1) comprising: the medium is attached to the aqua tikum tumefaciens strain in odorless of methyl tumefaciens aqua tikum (Methylobacterium aquaticum) with methyl in the coated surface, and
(2) culturing the above-mentioned odorless strain of Methylobacterium aquaticum in aerobic condition to form a biofilm;
Wherein the method comprises the steps of:
11. The method of claim 10, with methyl in the above step (1) tumefaciens aqua tikum tumefaciens beuraki Atum (Methylobacterium brachiatum) methyl addition (Methylobacterium aquaticum), bacterium methyl Solarium flash Tani (Methylobacterium platani), Acinetobacter Jones you (Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis), Breda ratio Bacillus inboard car tooth (Brevibacillus invocatus), Day Noko kusu blood Syracuse (Deinococcus ficus), grapefruits Sonia Solid (Leifsonia soli), Pseudomonas nitro Lowry dew sense (Pseudomonas nitroreducens), Sphingomonas aqua subtilis (sphingomonas aquatilis) and methyl tumefaciens coma the state (air-conditioning, comprising a step of coating the one or microorganism or more kinds selected from the group consisting of Methylobacterium komagatae) Additionally biofilm A method for manufacturing an odorless eva core which does not cause odor in the device.
11. The method of claim 10, wherein the surface of the core is made of aluminum, an aluminum alloy, copper, or a copper alloy, and does not cause odor in the air conditioner.
That is, by coating by breeding tumefaciens strain in aqua tikum odorless methyl of the EVA core of the air conditioner methyl tumefaciens aqua tikum (Methylobacterium aquaticum) under aerobic conditions not causing the odor in the air conditioner odorless biofilm.
The method of claim 13, wherein said odorless biofilm is in the methyl tumefaciens aqua tikum (Methylobacterium aquaticum) in addition tumefaciens beuraki Atum methyl (Methylobacterium brachiatum), bacterium methyl Solarium flash Tani (Methylobacterium platani), Acinetobacter Jones you (Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis), Breda ratio Bacillus inboard car tooth (Brevibacillus invocatus), Day Noko kusu blood Syracuse (Deinococcus ficus), grapefruits Sonia Solid (Leifsonia soli), Pseudomonas nitro Lowry dew sense (Pseudomonas nitroreducens), Sphingomonas aqua subtilis (sphingomonas aquatilis) and methyl with the air conditioning device, comprising a step tumefaciens coma the coating further comprises one or two microorganism or more kinds selected from the group consisting of a condition (Methylobacterium komagatae) Odorless biofilm that does not cause my odor.
Methyl tumefaciens aqua tikum (Methylobacterium aquaticum) of the air-conditioning apparatus that does not cause the smell coated with a biofilm formed by the propagation tumefaciens strain in aerobic conditions to Aqua tikum odorless methyl.
The method of claim 15, wherein the cooling device is the methyl tumefaciens aqua tikum (Methylobacterium aquaticum) in addition tumefaciens beuraki Atum methyl (Methylobacterium brachiatum), tumefaciens flash Tani (Methylobacterium platani) methyl, Acinetobacter Jones Needle ( Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis), Breda ratio Bacillus inboard car tooth (Brevibacillus invocatus), Day Noko kusu blood Syracuse (Deinococcus ficus), grapefruits Sonia Solid (Leifsonia soli), Pseudomonas nitro Lowry dew sense (Pseudomonas nitroreducens ), Sphingomonas aqua subtilis (sphingomonas aquatilis) and the Solarium coma bacterium methyl causing the odor, comprising a step of coating further includes the one or microorganism or more kinds selected from the group consisting of a condition (Methylobacterium komagatae) Do not let the air conditioner.
16. The air conditioning system of claim 15, wherein the air conditioning system includes an eva core.

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