KR101698869B1 - A composition for treatment of Atopic dermatitis containing oriental medicine herbs - Google Patents

Abstract

The present invention relates to a composition for preventing or treating atopic dermatitis, which contains an oriental medicinal herb as an active ingredient. The present invention relates to a composition for preventing or treating atopic dermatitis, which comprises an extract of an oriental medicinal herb including an obovate, a branch, a casualty, , It can safely be used without side effects unlike conventional medicines such as steroids and antihistamines, while effectively suppressing skin rash caused by atopic dermatitis.

Description

Technical Field [0001] The present invention relates to a composition for preventing or treating atopic dermatitis,

The present invention relates to a composition for preventing or treating atopic dermatitis, which contains, as an active ingredient, a herb medicine which can be safely used without adverse effects unlike conventional medicines such as steroids and antihistamines while effectively suppressing skin rash caused by atopic dermatitis.

Atopic dermatitis is a chronic skin disease characterized by epidermal hyperplasia, erythema, edema, severe pruritus, exudation and erythema as well as skin dryness. The cause of atopic dermatitis has not yet been clarified, but it is known that genetic factors, immune function imbalance and environmental factors are related.

The atopic dermatitis is chronic recurrent skin disease, and it is prevalent in 10 ~ 20% of children and 1 ~ 3% of adults in most developing countries including Korea. Recently, prevalence of Allergy and Allergy has been increasing in domestic and foreign countries.

Atopic dermatitis, which is increasing due to increased pollution due to rapid industrialization, is accompanied by skin dryness, eczema, erythema and papules. Secondarily, it affects physical and mental development and can reach skin cancer.

However, antihistamines, topical corticosteroids, immunosuppressants, and steroids have been used, but their use is very limited due to side effects such as liver function.

The most common atopic dermatitis inducers are IgE and histamine excess secretion, cellular immunodeficiency, and the increase of active factors involved in inflammatory reaction. Thus, chemical medication such as steroids, antihistamines, and antimicrobials have been popularized.

The above steroids (adrenocorticotropic agents) are largely anti-inflammatory and immunosuppressive and have excellent effects. However, when used for a long period of time, various adverse effects such as skin pigmentation, skin keratinization, abdominal pain and fever may occur. In addition, antihistamines reduce the symptoms of an itch by inhibiting the release of histamine in mast cells, but they are not a fundamental treatment method and are used as a temporary measure. Likewise, long-term administration may have side effects such as insomnia, anxiety and loss of appetite have.

The causes of atopy are so wide that it is impossible to treat the causes of atopy as a partial treatment function for each technical field such as antioxidation, antibacterial activity, anti-inflammation, immunity control and the like.

Therefore, there is a demand for materials using natural materials which can be widely applied before the main functional groups and have a small side effect.

Korea Patent Publication No. 2006-0033069 Korea Patent Publication No. 2013-0131644

The object of the present invention is to provide a composition capable of preventing or treating atopic dermatitis containing an extract extracted from an oriental medicinal herb including an obovate, a branch, a casualty, a herb, a mold, a ginseng, a rhubarb, have.

Another object of the present invention is to provide a cosmetic composition which can prevent or ameliorate atopic dermatitis by containing the herbal medicine extract as an active ingredient.

It is still another object of the present invention to provide a health functional food containing the herbal medicine extract as an active ingredient and capable of preventing or ameliorating atopic dermatitis.

It is still another object of the present invention to provide a hygiene product containing the herbal extract as an active ingredient and capable of preventing or ameliorating atopic dermatitis.

In order to achieve the above object, the present invention provides a composition for preventing or treating atopic dermatitis, which comprises an extract from an oriental medicinal herb containing as a active ingredient, at least one selected from the group consisting of a rhubarb, a pedestrian, a casualty, a rhizome, can do.

The herbal extract may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

1: 1-5: 1-5: 1-5: 1-5: 1-5: 1-5: 1-5: 1: 1-5: 1-5: 1-5: 1-5: 1-5: 1-5: 1-5 : 1-5: 1-5.

The main organic compounds contained in the medicinal herb extracts may include gallic acid, caffeic acid, and hyperoside.

The major organic compounds contained in the medicinal herb extracts are gallic acid of 52.0 to 54.0 mg / g, caffeic acid of 13.0 to 14.0 mg / g and hyperoside of 16.0 to 18.0 mg / g. . ≪ / RTI >

According to another aspect of the present invention, there is provided a cosmetic composition for prevention or improvement of atopic dermatitis, which comprises an extract from an oriental medicinal herb including dicotyledonous, epidermis, casualty, obtusa, moldi, gossam, rhubarb, May be contained as an active ingredient.

According to another aspect of the present invention, there is provided a health functional food for preventing or ameliorating atopic dermatitis, comprising at least one compound selected from the group consisting of dicotyledonosaccharide, lipase, caspase, Extract may be contained as an active ingredient.

According to another aspect of the present invention, there is provided a sanitary article for prevention or improvement of atopic dermatitis, comprising at least one of extracts extracted from herbal medicines containing an obsidian, a lord, a casualty, an obturator, a fungus, a ginseng, a rhubarb, As an active ingredient.

The composition for preventing or treating atopic dermatitis of the present invention has excellent therapeutic effect in animal models of skin dryness, eczema, erythema and papules caused by atopic dermatitis. Can be used safely.

FIG. 1A is a graph showing an HPLC analysis of an organic compound contained in the herbal extract prepared according to Example 1 of the present invention. FIG.
FIG. 1B is a table showing the content of organic compounds analyzed by HPLC in FIG. 1A.
FIG. 2A shows paraffin fixation after obtaining dorsal skin tissues from the control group, the atopy group, the Example 1 treatment group, and the dexamethasone treatment group. The thickness of the skin slice was measured by Focus pro program after H & E staining of fixed tissue.
FIG. 2B is a photograph showing the extent of mast cell infiltration of the epidermis and dermis of the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.
FIG. 2C is a graph showing the skin thickness measured in FIG. 2A.
Figure 2D is a graphical representation of the dermal thickness measured in Figure 2A.
FIG. 2E is a graph showing the degree of mast cell infiltration measured in FIG. 2B.
FIG. 3A is a graph showing the values of IgE in the control group, the atopy group, the Example 1 treatment group, and the dexamethasone treatment group.
FIG. 3B is a graph showing histamine levels of the control group, the atopy group, the Example 1 treatment group, and the dexamethasone treatment group.
4A is a graph showing TNF-a levels in the control, atopic, Example 1 and dexamethasone treated groups.
4B is a graph showing IL-6 levels of the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.
FIG. 4C is a graph showing the IL-1? Levels of the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.
FIG. 5A is a Western blot technique for inhibiting JNK, Erk, and p-38 in the control group, the atopy group, the Example 1 treatment group, and the dexamethasone treatment group.
Figure 5B is a graphical representation of the transcription factor levels using the Western blot technique of Figure 5A above.
6A is a graph showing IL-13 values in the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.
FIG. 6B is a graph showing the LL-4 values of the control group, the atopy group, the Example 1 treatment group, and the dexamethasone treatment group.
FIG. 6C is a graph showing the LL-5 values of the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.

The present invention relates to a composition for preventing or treating atopic dermatitis, which contains, as an active ingredient, a herb medicine which can be safely used without adverse effects unlike conventional medicines such as steroids and antihistamines while effectively suppressing skin rash caused by atopic dermatitis.

Hereinafter, the present invention will be described in detail.

The composition for preventing or treating atopic dermatitis according to the present invention contains an extract extracted from an oriental medicinal herb including an obovate, a branch, a casualty, an obturator, a mold, a ginseng, a rhubarb, a grass,

Rhus javanica Linne is an insect house of Pemphigidae, which is mainly parasitic on the leaves of Anacardiaceae, and is divided into two parts according to its appearance. In addition, it has been reported that in the Gobo Hwangcho, the 'dwarf bark' is good for the skin diseases of the genital part, and the skin disease caused by the wind poison penetrating the lungs, Itch, symptoms of flowing water, symptoms that do not stop bleeding in the hemorrhoids, and facial skin diseases caused by malnutrition in children. "

In addition, Kochia scoparia Schrader is a ripe fruit of Chenopodiaceae, which is dried in the sun in autumn. Taste is used and the quality is cold. In pharmacological experiments, liver protection, diuretic, detoxifying and antibacterial activity have been revealed. In addition, it is used for urine discomfort, heat (淋), edema, urticaria, eczema, malignant swelling, cystitis.

The paper supplier is mixed at a weight ratio of 1 to 5 on the basis of dwarf 1, and when the content of the paper supplier is less than the lower limit, the effect of preventing or treating atopic dermatitis may be low, and if it exceeds the upper limit value, skin dryness may be caused.

Also, casualties ( Cnidium monieri Cuss) is a fruit of Umbelliferae. It is the fruit of the genitalia of the female genitalia, which is swollen and sick, the male's shade (yin), the genital area is moist and itchy, It is said that it not only treats epilepsy and swelling, but also lighter when taken for a long period of time.

The casualties are mixed at a weight ratio of 1 to 5 on the basis of dwarf 1. If the casualties are below the lower limit, the itching of atopic dermatitis may not be suppressed. If the upper limit is exceeded, the itching of atopic dermatitis is rather increased .

In addition, Houttuynia cordata Thunberg is the top part of the Saururaceae and is listed in the separate record as "Treating the swelling of the swollen worm body fluid." In the zone name 池 大 明, And it is applied to baldness that does not heal well. "It is said that 'Lee Chin-Jin' treats hemorrhoids and debris by eliminating heat poisoning and swelling, treats the quality of medicines, Treat skin diseases ".

The herringbone is particularly used together with dwarf and mold to have excellent effects on atopic dermatitis. If the content of the herringbone is less than the lower limit, the effect of preventing or treating atopic dermatitis may be little. If the content of the herbicide exceeds the upper limit, eczema may be caused.

In addition, Schizonepeta tenuifolia Briquet is a flower bud of Labiatae. It treats skin itching, dizziness, swelling and vomiting caused by female blood circulation , And 'Meng 詵' is described as 'taking the powder to the symptom that the body is hardened by the invasion of the cold after the birth, and taking it as a liquor.' In Lee Ji-jin, (风气) to remove the scars of the head and eyes to clear the throat (throat) comfort and to improve the swelling, as well as the back of the neck stiffness, black stars in the field, And treats the vomiting of the vagina and treats the symptoms of vomiting blood, nosebleeds, hemorrhages, diarrhea accompanied by bleeding, bruises and hemorrhoids. '

The molds have excellent effects on atopic dermatitis, in particular, when used in combination with a rhubarb, rhubarb, gossam, rhubarb and Gaza. The molds are mixed at a weight ratio of 1 to 5 on the basis of 1 part by weight of daphnia. When the content of the mold is less than the lower limit, the effect of preventing or treating atopic dermatitis may be insignificant. Can be degraded.

In addition, Sophora flavescens Aiton is a root of leguminosae, which is used as it is or is removed from the juniper. It is effective in the gastroenteritis, enteritis, bacterial dyslipidae, and it is effective when it is dalyeoseo such as licorice, and it is effective also for the epidemic and eczema of the skin. Even when the itching is severe in the vulva of the wife, do. Skin ptsukyunbun (白 菌菌) is a combination of the (枯 白 고) and ointment is made by attaching to the affected area. It is used as a cleanser or pills because of its taste. It is not used as a bath salt. It is used for pyrexia, so it is best not to use it when the abdomen and body are cold. In the private sector, it is widely used that maggots can not grow if they are put in the toilet.

The ginseng is mixed at a weight ratio of 1 to 5 on the basis of 1 part by weight of ginseng. When the content of ginseng is lower than the lower limit, there is little effect of preventing or treating atopic dermatitis. If the ginseng content is above the upper limit, side effects may occur have.

In addition, Rheum palmatum Linne is the root and rootstock of Polygonaceae and has been removed. The effect of excessive heat accumulated in the body to lower the skin, constipation, constipation, hypertension, treatment of hyperemia and pain. It is also used for early symptoms of dysentery. It is also effective for fever, nosebleeds and acute jaundice caused by heat. It is widely used for symptoms such as fever, pain, severe redness, and hot burns. In addition, when the flour is applied to the bronchial flora which is generated in winter, it is excellent in anti-inflammatory action and the wound is easily healed. Recent clinical results showed that hemostatic effect was excellent for thrombocytopenic diseases, and 1225g of oral salt, cleft ulcer, and folliculitis were applied to the mouth, watered, flipped, groomed and cleaned for 45 times a day Healed. It is treated without any adverse effect if it is sprayed on the root with the old lime in the hot water burn. In the animal experiment, it has been proved that the anti - cancer action and anti -

The rhubarb is mixed at a weight ratio of 1 to 5 on the basis of dwarf 1, and when the content of rhubarb is less than the lower limit, the effect of preventing or treating atopic dermatitis is insignificant. If the content is above the upper limit, skin trouble may occur.

In addition, the self-inflicted (Lithospermum erythrorhizon Siebold et Zuccarini is described as a root of Boraginaceae, which treats 'hungry, injured and sick' in the separate list and heals plaster and swelling of the child by making plaster It is said that the treatment of severe swelling and scabies (improvement) is given to the right of vigilance, and Lee Ji-jin describes the treatment of poison of skin rash and pox pox, And make the colon (colon) smooth. "

The seedlings are mixed at a weight ratio of 1 to 5 on the basis of 1 part by weight of daphnid. When the content of seedlings is less than the lower limit, the effect of preventing or treating atopic dermatitis may be low, and if it exceeds the upper limit, erythema may be caused.

Also, let (Terminalia chebula Retzins) is a ripe fruit of the Combretaceae, which is effective for lowering the 气, removing old fences and coughing, breathing, old diarrhea, dysentery, drowning, dehydration. The phagocytosis of phagocytosis, branching, and P. aeruginosa was reported.

The ghazi are mixed at a weight ratio of 1 to 5 on the basis of dhabi. When the content of ghabi is below the lower limit, the effect of preventing or treating atopic dermatitis may be lowered. If the content is above the upper limit, skin itching may not be inhibited .

In addition, the above-mentioned silkworm ( Trichosanthes kirilowii Maximowicz) will remove the skin as the roots of watermelon and (Cucurbitaceae). It treats fever (heat crab), epidemic disease, communicates small intestine, sinks swollen, treats boils on breast and back, treats hemorrhoids, lymphadenitis, etc. It has the action of discharging and raising newborns and eliminating the eosinophilia caused by trauma. '

If the content of the silk powder is less than the lower limit, the effect of preventing or treating atopic dermatitis may be lowered. If the content of silk powder is less than the lower limit, skin trouble may occur. .

1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1 ratio is used for the prevention or treatment of atopic dermatitis, 1 by weight.

The herbal medicines are mixed with an extraction solvent at a weight ratio of 1: 5 to 25, preferably 1: 8 to 15, and extracted at 70 to 100 ° C to prepare an extract. When the weight ratio of the herbal medicine to the extraction solvent is out of the above range, the effective ingredient of the herbal medicine may be extracted in a small amount. If the extraction temperature is lower than the lower limit, the effective ingredient of the herbal medicine may not be extracted. If the extraction temperature is higher than the upper limit, the effective ingredient of the herbal medicine may be extracted in a smaller amount.

Water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used as the extraction solvent for extracting the medicinal herb extract, so that the medicinal herb extract can be preferably used for preventing or treating atopic dermatitis. The mixed solvent is not particularly limited, but preferably 20 to 80% by volume of an aqueous solution of methanol, ethanol, butanol or propanol, more preferably water.

The term " extract " used in referring to herbal medicine in the present specification includes not only crude extract obtained by treating an extracting solvent, but also processed products of herbal medicine extract. For example, the herbal extract can be prepared in powder form by an additional process such as vacuum distillation and lyophilization or spray drying.

In addition, the herbal medicine extract of the present invention has broad meaning as including a processed product of herb medicine, for example, herbal medicine powder, which is formulated so that the herb medicine can be administered to an animal. Although the present invention has been carried out with the herbal medicine extract, it can be expected that those skilled in the art can achieve the desired effects in the same manner as the herbal medicine product.

As used herein, the term " comprising as an active ingredient " means an amount sufficient to achieve the efficacy or activity of the herb extract. For example, the herbal medicine extract is used at a concentration of 10 to 1500 μg / ml, preferably 20 to 500 μg / ml. Since the herbal medicine extract has no adverse effect on the human body even when administered in an excessive amount as a natural product, the quantitative upper limit of the herbal medicine extract contained in the composition of the present invention can be selected by a person skilled in the art within a suitable range.

The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.

Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile water and sterile water suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

The pharmaceutical composition of the present invention can be administered orally or parenterally. In the case of parenteral administration, the composition can be administered by intravenous injection, subcutaneous injection, muscle injection, intraperitoneal injection, transdermal administration, etc., .

The appropriate dosage of the pharmaceutical composition of the present invention varies depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient, Usually, a skilled physician can readily determine and prescribe dosages effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g / kg.

The pharmaceutical composition of the present invention can be prepared in unit dose form by formulating it with a pharmaceutically acceptable carrier and / or excipient or can be manufactured by inserting it into a multi-dose container. The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

The present invention provides a food composition for preventing or treating atopic dermatitis containing an extract of a medicinal herb as an active ingredient.

The food composition according to the present invention can be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectioneries, diet bars, dairy products, meat, chocolates, pizza, ram noodles, other noodles, gums, ice cream, .

The food composition of the present invention may contain not only the herbal medicine extract as an active ingredient but also a component that is ordinarily added at the time of food production, and includes, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavors. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings. For example, when the food composition of the present invention is prepared from a drink and a beverage, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, various plant extracts and the like may be further added in addition to the herbal medicine extract of the present invention.

The present invention provides a health functional food comprising a food composition for prevention or treatment of atopic dermatitis containing the herbal extract as an active ingredient. The health functional food is a food prepared by adding the herbal extract to a food material such as a beverage, a tea, a spice, a gum or a confection, or encapsulating, pulverizing or suspending it. Meaning, unlike general medicine, there is an advantage that there is no side effect that can occur when a food is used as a raw material for a long time. The health functional food of the present invention thus obtained is very useful because it can be ingested routinely. The amount of the herbal medicine extract added in such a health functional food can not be uniformly determined depending on the type of the health functional food to which it is added but may be added within a range that does not impair the original taste of the food, To 50% by weight, preferably 0.1 to 20% by weight. In the case of health functional foods in the form of pills, granules, tablets or capsules, they may be added usually in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

The present invention also provides the use of medicines for the prevention or treatment of atopic dermatitis or medicinal plant extracts for the production of foods. As described above, the herbal medicine extract can be used for the prevention or treatment of atopic dermatitis.

The present invention also provides a method of preventing or treating atopic dermatitis comprising administering to a mammal an effective amount of a medicinal herb extract.

The term "mammal " as used herein refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term "effective amount" refers to the amount of active ingredient or pharmaceutical composition that elicits a biological or medical response in a tissue system, animal, or human, as contemplated by a researcher, veterinarian, physician or other clinician, ≪ / RTI > inducing a reduction of the symptoms of the disease or disorder. The effective amount and the administration frequency for the active ingredient of the present invention can be changed according to the desired effect. Thus, the optimal dosage to be administered can be readily determined by those skilled in the art and will vary with the nature of the disease, the severity of the disease, the amount of active and other ingredients contained in the composition, the type of formulation, and the age, The age, body weight, sex, diet, time of administration, route of administration and fraction of the composition, duration of treatment, concurrent medication, and the like. In the prevention, treatment, or improvement of the present invention, it is preferable that the herbal medicine extract is administered at a dose of 0.001 g / kg to 10 g / kg once or several times a day.

In the therapeutic method of the present invention, the composition containing the herbal extract as an active ingredient can be administered orally, rectally, intravenously, intraarterially, intraperitoneally, intramuscularly, intrasternally, transdermally, topically, Lt; / RTI >

In addition, the cosmetic composition for preventing or treating atopic dermatitis containing the herbal medicine extract of the present invention as an active ingredient can be prepared in the form of a general emulsified formulation and a solubilized formulation. Cosmetics of emulsified form include nutrition lotion, cream, essence, etc., and cosmetics of solubilized form have softening longevity.

Suitable cosmetic formulations include, for example, solutions, gels, solid or kneaded anhydrous products, emulsions obtained by dispersing the oil phase in water, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) In the form of a dispersing agent, in the form of a cream, a skin, a lotion, a powder, an ointment, a spray or a conical stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition may further contain, in addition to the extract of the present invention or its fractions, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, , Water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And may contain adjuvants conventionally used in the cosmetics field, such as any other ingredients used.

In addition, the present invention provides a hygiene article for preventing or treating atopic dermatitis containing the herbal medicine extract of the present invention as an active ingredient. The sanitary product containing the herbal medicine extract of the present invention can be used as a sanitary napkin, a soap, a shampoo, a bath boom, and the like. It can be used as a preservative, a stabilizer, a wetting agent or an emulsifier, Salts or buffers for the control of osmotic pressure may also be used.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Example 1.

The medicinal herb containing 100 g of the dwarf, 100 g of the lumberjack, 100 g of the casualty, 100 g of the perennial herb, 100 g of the mold, 100 g of the ginseng, 100 g of the rhubarb, 100 g of garlic, 100 g of Ganoderma lucidum, And the mixture was extracted at 100 캜 for 100 minutes to prepare a herbal medicine extract.

Example 2.

The medicinal herb and water containing 100 g of dwarf, 300 g of dead, 300 g of casualties, 200 g of mold, 300 g of molds, 200 g of ginseng, 200 g of rhubarb, 300 g of garlic, 300 g of garlic, And the mixture was extracted at 100 캜 for 100 minutes to prepare a herbal medicine extract.

Comparative Example 1

The same procedure as in Example 1 was carried out except that a herbal extract was prepared without using molds.

Comparative Example 2 < tb > < tb > < SEP >

The same procedure as in Example 1 was carried out except that the seedlings, The herbal medicine extracts were prepared without using them.

<Test Example>

Preparation of experimental animals and preparation of samples

1-1. Experimental animal

The experimental animals used in this experiment were male NC / Nga mice (26-27 g) at 7 weeks of age and were supplied from Korea BioLink and adapted for 1 week in an experimental animal husbandry. During the experimental period, solid feed and water were freely consumed. The temperature of the breeding room (22 ± 2 ℃), relative humidity (60 ± 5%) and intensity were maintained for 12 hours. On the day before the start of the experiment, the entire area of the back of the animal from the lower part of the neck to the upper part of the tail was removed with an electric razor, and then the cream hair removal agent was applied to completely remove the hairs so that the skin was clearly exposed. This animal experiment was conducted in accordance with the provisions of the Animal Experiment Ethics Committee under the prior deliberation of the Daejeon University Animal Experimental Ethics Committee.

1-2. Sample preparation

DNCB (Sigma-Aldrich, St. Louis, Mo., USA) was dissolved in a solvent (acetone: olive oil = 3: 1) at a concentration of 0.2% and 0.4%.

Test Example 1. Analysis of organic compounds

The organic compound components contained in the herbal extract prepared according to Example 1 were analyzed using an Agilent 1100 series HPLC instrument (Agilent Technologies, USA). We used chemstation software (Agilent Technologies, U.S.A.) and autosampler (G1313A), column oven (G1316A), binary pump (G1312), DAD detector (G1315B) and degasser

FIG. 1A is a graph showing an analysis of an organic compound contained in an extract of herbal medicines prepared according to Example 1 of the present invention by HPLC; 1B is a table showing the content of organic compounds analyzed by HPLC.

As shown in FIG. 1, it was confirmed that the herbal medicine extract of Example 1 contains gallic acid, caffeic acid and hyperoside as effective substances.

Test Example 2. Cytotoxicity measurement

The cytotoxicity of the extracts prepared in Examples and Comparative Examples was measured.

Cytotoxicity was measured by the method of Rochem et al. In order to carry out the test in the safety concentration range of the sample. Cells (4 × 10 ⁵ cells / well) (RBL-2H3) were plated on 48-well plates and cultured for 24 hours. Then, 20 uL of the extract dissolved in the medium was dispensed and cultivated for 12 hours. Twelve hours later, 20 uL of WST-8 solution was added to each well and incubated for another 2 hours. The cytotoxicity was measured at 450 nm using an ELISA Reader and the safety concentration range was determined. The medium used was MEM containing 15% (v / v) fetal bovine serum (FBS) and 1% (v / v) penicillin and the culture was maintained at 37 ° C, 5% CO _2, 95% humidified Incubator. The concentration of the extract 1 [mu] g / ml.

division Example 1 Example 2 Comparative Example 1 Comparative Example 2 Cell viability (%) 99.5 99.2 98.3 98.1

As shown in Table 1, the herbal medicine extracts prepared according to the examples and the comparative examples of the present invention all showed excellent cell viability.

Test Example 3. Effect on atopic dermatitis

All animals were given free diet and drinking water, and 8 animals were assigned to each group. 3 DNCB-treated normal group (control group), 3 atopy group applied DNCB on the back part, 8 drug groups applied with 25 mg / ㎖ of herbal medicine extract on their backs while applying DNCB, 50 (8 mg / ㎖) of herbal medicines, followed by DNCB and 8 mg of herbal medicine extracts at a concentration of 100 mg / ㎖. DNCB was applied and dexamethasone was applied at a dose of 3 mg / kg Marie.

Seven-week-old male NC / Nga mice were cleaned at the back and left to stand for 24 hours so that the micro-wounds of the skin that might occur during the hair removal process were healed. After 24 hours, 200 μl of 0.4% DNCB per mouse was applied to the back region for 3 weeks continuously to induce atopic dermatitis. DNCB application concentration was reduced to 0.2% and atopic dermatitis persisted. Samples were applied daily for 2 weeks at the same time after reducing the concentration of DNCB to 0.2%.

Seven weeks old male was sacrificed and orbital hemorrhage was performed to analyze peripheral blood.

Table 2 shows the values of WBC, Lymphocytes, Monocytes, Segmental Neutrophils, Eosinophil and Basophils. Table 2 shows the values of WBC, Lymphocytes, Monocytes, Segmental Neutrophils, Eosinophil and Basophils. The lymphocytes, monocytes, neutrophils, eosinophils and basophils are the finely divided types of leukocytes.

The lymphocytes produce antibodies capable of fighting germs and fighting various germs; The monocytes help neutrophils fight infection (antigen delivery); The neutrophil fights germs when infected with the body; The eosinophil mainly acts to resist allergies and parasites; The basophils are responsible for removing bacteria and other external bacteria from the immune side.

division K / l leukocyte Lymphocyte Monocyte Neutrophil Eosan Basophils Control group 2.35 1.68 0.06 0.59 0.01 0.01 Atopy group 3.54 1.97 0.07 1.33 0.12 0.05 Dexamethasone (3 mg / kg) 2.79 2.10 0.07 0.56 0.04 0.02 Example 1 25 mg / ml 3.55 2.19 0.07 1.18 0.08 0.03 50 mg / ml 2.79 1.83 0.07 0.78 0.08 0.03 100 mg / ml 2.96 2.21 0.06 0.59 0.08 0.02 Example 2 25 mg / ml 3.57 2.41 0.07 1.01 0.07 0.01 50 mg / ml 2.88 1.82 0.06 0.89 0.09 0.02 100 mg / ml 3.10 2.56 0.06 0.51 0.08 0.02 Comparative Example 1 25 mg / ml 3.58 2.17 0.07 1.21 0.10 0.03 50 mg / ml 3.26 2.12 0.06 0.98 0.09 0.01 100 mg / ml 3.49 2.46 0.06 0.84 0.10 0.03 Example 2 25 mg / ml 3.58 2.18 0.05 1.22 0.11 0.02 50 mg / ml 3.42 2.21 0.07 1.02 0.09 0.03 100 mg / ml 3.51 2.37 0.06 0.96 0.09 0.03

As shown in Table 2 above, eosinophils increase in leukocytes and leukocytes when atopic dermatitis is present. In the group of the herbal medicine extract prepared according to Example 1 of the present invention coated with 50-100 mg / ml, leukocytes and eosinophils Of the total amount of the product. This means that the herbal extract of Example 1 alleviates symptoms of atopic dermatitis.

On the other hand, the herbal medicine extracts of Comparative Examples 1 and 2 showed that the levels of leukocytes and eosinophils did not significantly decrease as compared with the atopic group, and thus they did not alleviate symptoms of atopic dermatitis.

Test Example  4. Measurement of skin thickness of mouse

Changes in the dorsal skin of the mice treated according to Test Example 3 were observed. In the atopic group, external changes such as erythema, scaling, scarring, and flaccidity similar to those of atopic symptoms were observed, and scratching of the area to which DNCB was applied was observed, and skin thickness was gradually thicker than normal skin I felt it.

It was confirmed how the dorsal skin thickness of the mice coated with the herbal medicine extract prepared in Example 1 was changed compared to the atopic group.

The dorsal skin thickness of the mouse was determined by dividing the dorsal skin tissue of the sacrificed female mouse and then the thickness of epidermis and dermis by H & E staining.

 FIG. 2A shows paraffin fixation after obtaining dorsal skin tissues from the control group, the atopy group, the Example 1 treatment group, and the dexamethasone treatment group. The thickness of the skin slice after H & E staining of fixed tissue was measured by Focus pro program; FIG. 2B is a photograph showing the extent of mast cell infiltration by staining the epidermis and dermis of the control, atopic group, Example 1 treatment group and dexamethasone treatment group with toluidine blue. After the paraffin section, the yellow arrow indicates the infiltration state of mast cells.

FIG. 2C is a graphical representation of the skin thickness measured in FIG. 2A; FIG. Figure 2D is a graphical representation of the dermal thickness measured in Figure 2A above; FIG. 2E is a graph showing the degree of mast cell infiltration measured in FIG. 2B.

As shown in FIG. 2, the thickness of the epidermis and dermis of the mouse injected with the herbal medicine extract prepared in Example 1 was found to be smaller than that of the atopic group. In particular, the group to which 100 mg / ml of herbal medicine extract (Example 1) was added in the epidermis was further reduced in thickness compared to dexamethasone. This means that the herbal medicine extract prepared in Example 1 relieves the symptoms of atopic dermatitis.

Test Example 5. Measurement of IgE and histamine

The hematopoietic blood from the abdominal vein of the sacrificed mouse according to Test Example 3 was centrifuged and serum levels of IgE and histamine were confirmed.

3A is a graph showing the values of IgE in the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group; FIG. 3B is a graph showing the histamine levels of the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.

As shown in FIG. 3, the levels of IgE and histamine were increased in the atopic group as compared with the control group, and it was confirmed that the levels of IgE and histamine were lowered by treatment with the herbal extract of Example 1.

Test Example  6. Inflammation Cytokine ( TNF -, lL -6 and lL -1) measurement

The levels of TNF-α, IL-6 and IL-1β were determined by sera obtained after centrifugation of blood from the abdominal vein of mice sacrificed according to Test Example 3 above.

4A is a graph showing TNF-a levels in the control, atopic, Example 1 and dexamethasone treated groups; 4B is a graph showing IL-6 levels in the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group; FIG. 4C is a graph showing the IL-1? Levels of the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.

As shown in FIG. 4, the levels of TNF-α, IL-6 and IL-1β were increased in the atopic group as compared with the control group, and it was confirmed that the treatment with the herbal composition composition of Example 1 lowered the level.

Test Example  7. Measurement of inflammatory response

The dorsal skin tissues of the mice sacrificed according to Test Example 3 were separated and the levels of transcription factors were measured using the Western blot technique. The results are shown in FIG. 5A.

Figure 5B also graphically depicts transcription factor levels using the Western blot technique.

As shown in FIG. 5, the values were increased in the atopic group as compared with the control group, and it was confirmed that the treatment with the herbal composition composition of Example 1 lowered the value. It can be seen that the symptoms of atopic dermatitis are suppressed by the herbal medicine extract of Example 1.

Test Example  8. lL -13, lL -4 and lL -5 measurement (ex- vivo )

In order to confirm the anti-inflammatory effect, the spleen of the sacrificed mouse according to Test Example 3 was isolated and ex-vivo experiment was conducted to obtain spleen cells.

6A is a graph showing IL-13 values in the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group; FIG. 6B is a graph showing the LL-4 values of the control group, the atopy group, the Example 1 treatment group, and the dexamethasone treatment group; FIG. 6C is a graph showing the LL-5 values of the control group, the atopic group, the Example 1 treatment group, and the dexamethasone treatment group.

As shown in FIG. 6, the herbal composition compositions of Example 1 showed low levels of lL-13, lL-4 and lL-5 in the atopic group. This means that the production of atopy-induced lL-13, lL-4 and lL-5 was markedly inhibited, thereby confirming that the herbal composition composition of Example 1 has an anti-inflammatory effect of atopic dermatitis.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Preparation Example 1. Preparation of powder

500 mg of the herbal extract powder obtained in Example 1

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation Example 2. Preparation of tablets

300 mg of the herbal extract powder obtained in Example 1

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation Example 3. Preparation of capsules

200 mg of the herbal extract powder obtained in Example 1

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation Example 4. Preparation of injection

600 mg of the herbal extract powder obtained in Example 1

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _4, 12H ₂ O 26 mg

It is prepared by the above-mentioned component content per ampoule according to the usual injection preparation method.

Formulation Example 5. Preparation of a liquid preparation

4 g of the herbal extract powder obtained in Example 1

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to a conventional liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and then purified water was added thereto. The entirety was adjusted to 100 g by adding purified water, And sterilized to prepare a liquid preparation.

Preparation Example 6 Preparation of Granules

1,000 mg of the herbal extract powder obtained in Example 1

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

0.2 [mu] g vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

The composition ratio of the above-mentioned vitamins and minerals is comparatively comparatively mixed with the granules according to the preferred embodiment. However, the blending ratio may be arbitrarily changed, and the above components are mixed according to the ordinary granule preparation method, Can be prepared and used in the manufacture of a health functional food composition according to a conventional method.

Preparation Example 7. Preparation of functional beverage

 1,000 mg of the herbal extract powder obtained in Example 1

Citric acid 1,000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to the flask to obtain a total of 900 mL

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 L container, It is used in the production of the functional beverage composition of the invention.

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Formulation Example 8. Preparation of cosmetic product of emulsified formulation

Cosmetics of emulsified form such as nutrient lotion, cream, essence, and cosmetics of solubilized form such as softened longevity were prepared.

Emulsifier type cosmetics were prepared with the compositions shown in Table 3 below. The production method is as follows.

1) A mixture of raw materials 1 to 9 was heated to 65 to 70 占 폚.

2) The starting material of 10 was added to the mixture of step 1).

3) The mixture of raw materials 11 to 13 was completely dissolved by heating at 65 to 70 ° C.

4) While the above step 3) was carried out, the mixture of step 2) was gradually added and emulsified at 8,000 rpm for 2 to 3 minutes.

5) The raw material of 14 was dissolved in a small amount of water and then added to the mixture of step 4) and further emulsified for 2 minutes.

6) The raw materials of 15 to 17 were each weighed, and then put into the mixture of step 5) and further emulsified for 30 seconds.

7) The mixture of step 6) was degassed after emulsification and cooled to 25-35 占 폚 to prepare an emulsifier-type cosmetic.

Furtherance Emulsifier type 1 Emulsifier type 2 Emulsifier type 3 One Stearic acid 0.3 0.3 0.3 2 Stearyl alcohol 0.2 0.2 0.2 3 Glyceryl monostearate 1.2 1.2 1.2 4 Wax 0.4 0.4 0.4 5 Polyoxyethylene sorbitan
Monolauric acid ester 2.2 2.2 2.2 6 Methyl paraoxybenzoate 0.1 0.1 0.1 7 P-hydroxybenzoic acid profile 0.05 0.05 0.05 8 Cetyl ethyl hexanoate 5 5 5 9 Triglyceride 2 2 2 10 Cyclomethicone 3 3 3 11 Distilled water ~ 100 ~ 100 ~ 100 12 Concentrated glycerin 5 5 5 13 Triethanolamine 0.15 0.15 0.15 14 Polyacrylic acid polymer 0.12 0.12 0.12 15 Pigment 0.0001 0.0001 0.0001 16 incense 0.10 0.10 0.10 17 The herbal medicine extract of Example 1 0.0001 One 10

Formulation example  9. Solubilization  Manufacture of Cosmetics for Formulation

Cosmetic products of the solubilized formulations were prepared with the compositions shown in Table 4 below. The production method is as follows.

1) 2 to 6 raw materials were put into 1 raw material (purified water) and dissolved using a mixer.

2) Raw materials 8 to 11 were completely dissolved in 7 raw materials (alcohol).

3) The mixture of step 2) was slowly solubilized by adding it to the mixture of step 1).

Furtherance Solubilization Formulation 1 Solubilization Formulation 2 Solubilization Formulation 3 One Purified water ~ 100 ~ 100 ~ 100 2 Concentrated glycerin 3 3 3 3 1,3-butylene glycol 2 2 2 4 EDTA-2Na 0.01 0.01 0.01 5 Pigment 0.0001 0.0002 0.0002 6 The herbal medicine extract of Example 1 0.1 5 5 7 Alcohol (95%) 8 8 8 8 Methyl paraoxybenzoate 0.1 0.1 0.1 9 Polyoxyethylene
Hydro genide ester 0.3 0.3 0.3 10 incense 0.15 0.15 0.15 11 Cyclomethicone - - 2

Claims (8)

A composition for preventing or treating atopic dermatitis characterized by containing as an active ingredient an extract derived from a medicinal herb including a hibiscus, a spouse, a casualty, a hibiscus, a hibiscus, a hibiscus, a rhubarb, The composition for preventing or treating atopic dermatitis according to claim 1, wherein the herbal extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. 4. The method according to claim 1, wherein 1: 1-5: 1-5: 1-5: 1-5: 1-5: 1: 1 -5: 1-5: 1-5: 1-5. The composition for preventing or treating atopic dermatitis according to claim 1, The composition for preventing or treating atopic dermatitis according to claim 1, wherein the main organic compound of the medicinal herb extract comprises gallic acid, caffeic acid and hyperoside. The method according to claim 1, wherein the main organic compound of the medicinal herb extract is selected from the group consisting of 52.0 to 54.0 mg / g of gallic acid, 13.0 to 14.0 mg / g of caffeic acid and 16.0 to 18.0 mg / A composition for preventing or treating atopic dermatitis, which comprises hyperoside. A cosmetic composition for prevention or improvement of atopic dermatitis characterized by containing, as an active ingredient, an extract extracted from a medicinal herb including a dwarf, a spouse, a casualty, a herb, a mold, a ginseng, a rhubarb, A health functional food for preventing or ameliorating atopic dermatitis characterized by containing, as an active ingredient, an extract extracted from a medicinal herb including a hibiscus, a spouse, a casualty, a hibiscus, a mold, a gosam, a rhubarb, A hygiene article for prevention or improvement of atopic dermatitis characterized by containing as an active ingredient an extract extracted from a medicinal herb including a dwarf, a spouse, a casualty, a herb, a mold, a ginseng, rhubarb, grass,

Images