KR101683371B1

KR101683371B1 - Control method for portable algae detecting apparatus and portable algae detecting apparatus

Info

Publication number: KR101683371B1
Application number: KR1020150062867A
Authority: KR
Inventors: 김정호; 이은희; 손아정; 김성훈; 박성민
Original assignee: 이화여자대학교 산학협력단
Priority date: 2015-05-06
Filing date: 2015-05-06
Publication date: 2016-12-06
Also published as: KR20160131133A

Abstract

A method of controlling a portable microalgae detecting apparatus includes a DNA detecting apparatus connected to a preprocessed DNA, a first probe and a second QD, which are connected to a first quantum dot (QD) Irradiating a sample solution containing a second probe binding to a second site of the DNA with a laser beam for a set time of a timer, the DNA detecting apparatus comprising a first QD and a second probe, The method comprising the steps of: receiving photons emitted from the QDs with different photodiodes; and when the output signal level of the photodiodes is lower than a reference value, the set time of the timer is increased to irradiate the sample solution with the laser light secondarily And the DNA detection device analyzes the output signal of the photodiode when the output signal level is equal to or greater than a reference value, Or performing a quantitative analysis.

Description

TECHNICAL FIELD [0001] The present invention relates to a portable micro-algae detecting apparatus and a portable micro-algae detecting apparatus,

The technique described below relates to a portable micro-algae detecting device and a DNA detecting method using the portable DNA detecting device.

A method for analyzing biochemical materials is generally used, such as an electrochemical method for performing analysis through measurement of electrical signals and a spectroscopic analysis method for performing analysis by measuring light such as fluorescence or chemiluminescence. Among these, spectroscopic analysis method which performs analysis using light is excellent in analytical sensitivity, and thus is an analytical method widely used for analysis of trace amounts of biochemical materials.

In order to perform fluorescence analysis, light is irradiated from the outside to the analytical sample solution, and fluorescence is detected at an angle perpendicular to the irradiation path of the light to perform analysis. And mass spectrometry or quantitative analysis is performed using a device such as a spectrofluorometer for analyzing the light emitted from the sample. A fluorescence spectrophotometer utilizes the inherent optical properties of a particular material.

US Patent Publication No. US2001-0028458 U.S. Pat. No. 5,500,536

Fluorescence spectrophotometers are used in material analysis by placing them in laboratories. They are large in size and high in price. Since the fluorescence spectrophotometer is difficult to carry, it is used only for the specific material analysis in the laboratory where the device is placed.

A technique to be described below is to provide a substance detection apparatus for analyzing a photon that is irradiated with light after probing a target substance to be analyzed with a fluorescent substance. The technique described below is intended to provide a portable analyzer capable of analyzing a target material on which a fluorescent material is probed. In particular, the technique described below is intended to provide a method or apparatus for qualitative analysis and / or quantitative analysis of microalgae DNA while efficiently using the energy of a portable analyzer.

The solutions to the technical problems described below are not limited to those mentioned above, and other solutions not mentioned can be clearly understood by those skilled in the art from the following description.

A method for controlling a portable microalgae detecting apparatus includes a DNA probe connected to a pre-processed DNA, a first QD (quantum dot) that is a fluorescent material, a first probe that binds to a first site of DNA, Irradiating the sample solution containing the second probe with a laser beam for a set time of a timer for a set time of the timer, irradiating the first QD receiving the laser light and the photon emitted from the second QD A step in which the DNA detection device irradiates the laser light to the sample service by increasing the set time of the timer when the output signal level of the photodiode is lower than the reference value, And analyzing the output signal of the photodiode to perform qualitative analysis or quantitative analysis of the microalgae DNA when the value is equal to or more than the reference value.

The DNA detecting apparatus can irradiate the laser light while increasing the set time of the timer by the reference unit repeatedly until the output signal level becomes equal to or greater than the reference value when the output signal level of the photodiode is less than the reference value.

The portable microalgae detecting device includes a sample holder in which a sample containing DNA having a fluorescent substance is placed, a light source device that irradiates light to the sample during a set time of the timer, and a sensor device And controlling the light source device to irradiate the sample with light when the output signal level of the sensor device is less than the reference value, and controlling the light source device to irradiate the sample with light based on the wavelength of the photon and the amount of the photon received by the sensor device And a control circuit for qualitative analysis or quantitative analysis of the microalgae DNA contained in the sample.

The control circuit may control the light source device to repeatedly irradiate the light while increasing the set time of the timer by the reference unit until the output signal level becomes equal to or greater than the reference value when the output signal level of the sensor device is less than the reference value.

The control circuit can perform the analysis based on the average value of the output signal levels measured plural times and the output signal level of the sensor device plural times when the output signal level is equal to or higher than the reference value.

The technology described below provides a DNA analyzer having a small size and low cost as compared with the conventional fluorescence spectrophotometer. Further, the technique described below efficiently utilizes the limited energy of the portable assay field and allows relatively long DNA analysis without additional energy supply.

The effects of the techniques described below are not limited to those mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the following description.

Figure 1 is an illustration of target DNA and QD.
2 is an example of a block diagram showing the configuration of a portable microalgae detecting apparatus.
3 is another example of a block diagram showing the configuration of a portable microalgae detecting apparatus.
4 is a flowchart of a method for DNA analysis using a portable microalgae detecting apparatus.
5 is an example of a flowchart for a method for controlling a portable microalgae detecting apparatus.

The following description is intended to illustrate and describe specific embodiments in the drawings, since various changes may be made and the embodiments may have various embodiments. However, it should be understood that the following description does not limit the specific embodiments, but includes all changes, equivalents, and alternatives falling within the spirit and scope of the following description.

The terms first, second, A, B, etc., may be used to describe various components, but the components are not limited by the terms, but may be used to distinguish one component from another . For example, without departing from the scope of the following description, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component. And / or < / RTI > includes any combination of a plurality of related listed items or any of a plurality of related listed items.

As used herein, the singular " include "should be understood to include a plurality of representations unless the context clearly dictates otherwise, and the terms" comprises & , Parts or combinations thereof, and does not preclude the presence or addition of one or more other features, integers, steps, components, components, or combinations thereof.

Before describing the drawings in detail, it is to be clarified that the division of constituent parts in this specification is merely a division by main functions of each constituent part. That is, two or more constituent parts to be described below may be combined into one constituent part, or one constituent part may be divided into two or more functions according to functions that are more subdivided. In addition, each of the constituent units described below may additionally perform some or all of the functions of other constituent units in addition to the main functions of the constituent units themselves, and that some of the main functions, And may be carried out in a dedicated manner. Therefore, the existence of each component described in the present specification should be interpreted as a function. For this reason, the configuration of the portable micro-algae detecting apparatus or the portable micro-algae detecting apparatus described below will be described with reference to the following description It is clear that it can be different from the corresponding drawings in the extent that the object can be achieved.

Also, in performing a method or an operation method, each of the processes constituting the method may take place differently from the stated order unless clearly specified in the context. That is, each process may occur in the same order as described, may be performed substantially concurrently, or may be performed in the opposite order.

Hereinafter, a method for controlling a portable microalgae detecting device or a portable microalgae detecting device will be described in detail with reference to the drawings.

The techniques described below relate to an apparatus or method for detecting DNA in a biological material. The technique described below relates to a portable DNA detection device that performs qualitative analysis and / or quantitative analysis of DNA, particularly in pre-treated and post-treated sample solutions. The technique described below relates to a method for controlling a portable DNA detection apparatus so that DNA analysis can be appropriately performed.

Sample solution preparation

First, a preparation process of a sample solution including DNA (Deoxyribonucleic acid) will be described briefly. DNA pretreatment and post-processing are briefly described.

Generally, green tide refers to cyanobacteria (blue-green algae) that occur in rivers in summer. DNA should first be extracted from cyanobacterial samples. A variety of techniques commonly used in the art may be used for the DNA extraction process. The types of substances or DNAs that can be analyzed are not limited, but will be described below by way of example of green alga DNA. However, the technique described below can be used not only for microalgae including algae but also for DNA detection of other organisms.

Since the complementary two strands of the extracted DNA have a base pair of hydrogen bonds, DNA must first be bound to two strands of DNA (denatured DNA). Figure 1 is an illustration of target DNA and QD. In FIG. 1, the target DNA is currently treated with one strand.

In the technique described below, the fluorescent material uses a quantum dot (QD). QD is a nanocrystal material used in various fields. QD generally refers to a device made of a semiconductor material and having a nanometer-sized (1 to 250 nm) diameter. In FIG. 1, a spherical QD is shown, but it may have various shapes. For DNA detection, the QD is composed of a substance having fluorescence or contains a fluorescent substance.

FIG. 1 shows an example in which two QD's are used, and two QD's are bound to different probe DNAs. When QD ₆₅₅ bound to the first probe DNA corresponds to a fluorescent material mainly emitting 660 nm (red) wavelength photons, the QD ₅₆₅ bound to the second probe DNA mainly emits 540 nm (green) Emitting fluorescent material. In Fig. 1, two QDs are used, and each QD is a fluorescent material of a specific wavelength. However, for actual DNA detection, at least two QDs can be used, and fluorescent materials emitting photons of different wavelengths can be used. However, for the sake of explanation, red wavelength and green wavelength are contrasted more clearly.

QD ₅₆₅ shows a form coupled to a magnetic bead (MB) having a size larger than QD. MB is a material with magnetic properties, and QD ₅₆₅ is sufficient to bind MB in its surrounding form or in various forms. MB is used to separate the target DNA from other DNA or other substances in the sample during the DNA detection process.

The sample solution includes buffer solution, QD ₅₆₅ -MB-second probe DNA complex, QD ₆₅₅ -first probe complex, target DNA and the like. It is assumed that each probe binds to the complementary site of the target DNA. Then, when a magnetic field is generated at the position of the sample solution, the MB having magnetism moves to a certain region along the magnetic field. Eventually, the target DNA is concentrated in a specific region in the sample solution. Thereafter, a sample located in the specific region is collected, and the collected DNA can be subjected to a DNA detection process. As will be described later, in some cases, the process of concentrating DNA samples through magnetic field generation may be performed in a portable DNA detecting device. In this case, it may be desirable to allow light to be irradiated onto the region where the target DNA is concentrated.

For the sake of convenience of explanation, the probe associated with the extraction and detection of the target DNA in combination with the MB is referred to as a second probe, and probes which are only involved in the target DNA detection by binding only with a specific QD (QD _{655 in the} above description) Continue to call it a probe.

Portable DNA detection device

It is assumed that the sample solution containing the target DNA and QD has undergone a certain pre-treatment and post-treatment, and then an apparatus for detecting and quantifying the target DNA will be described.

Fig. 2 is an example of a block diagram showing the configuration of the portable microalgae detecting apparatus 100. Fig.

The portable microalgae detecting apparatus 100 receives light from a light source device 110 for irradiating light to a sample, a sample holder 120 to which a sample containing a green color DNA with a fluorescent substance attached is located, The algae DNA contained in the sample is quantitatively analyzed based on the wavelength of the photons and the amount of photons received by the photosensor 130 connected to the photosensor 130 and the photosensor 130 receiving the photons emitted by the sample And a control circuit (140)

The light source device 110 may be a halogen lamp, a xenon lamp, a high-intensity discharge (HID) lamp, one or more LEDs, one or more lasers, and the like. Further, the light source device 110 may use a plurality of light sources having different emission wavelength ranges for exciting a plurality of different fluorescent dyes in the biological sample. However, for convenience of explanation, it is assumed that the light source device 110 uses a semiconductor laser diode. Hereinafter, it is referred to as a laser diode. A laser diode is a small-sized device that emits a laser. Semiconductor laser diodes are a type of conversion device that converts current into coherent light, and have a feature that the power consumption is relatively small and the wavelength selection range is wide. If QD ₅₆₅ and QD ₆₅₅ are used, laser diodes may be suitable for irradiating 405 nm laser. The output wavelength, output intensity, output time, power value, etc. of the light source device 110 may have various values depending on the type of the sample to be detected.

The sample holder 120 is a structure in which a container such as a plate or a tube containing a sample solution is located. A variety of mechanical devices may be used depending on the size and shape of the container used. Or the sample holder 120 itself may have a container form capable of holding the sample solution. It will be important for the sample holder 120 to have a position where the sample solution can accurately receive the light irradiated by the light source device 110 and emit the photon to the nearby optical sensor 130 well.

The optical sensor 130 senses photons (fluorescence) emitted from the sample solution located in the sample holder 120. The optical sensor 130 may use various types of sensor elements capable of sensing photons. However, it is assumed that a photodiode having a small size is used in order to configure the portable microalgae detecting device 100 as a portable size. The photodiode may use a simpler PN photodiode, or a pin diode that allows a smaller light sensation than a PN photodiode.

2, optical sensor 130 includes optical sensor A 131 and optical sensor B 132. The reason why two optical sensors are shown is that the sample solution contains two QDs emitting different wavelengths as described above. Thus, each of the two photosensors senses light of different wavelengths. Assuming that QD ₅₆₅ and QD ₆₅₅ are included in the sample solution, the photosensors 131 and 132 detect light of wavelengths of 660 nm and 540 nm, respectively.

2, the control circuit 140 can detect (quantitatively analyze) the target DNA or quantify (quantitatively analyze) the amount of the target DNA based on the signal output from the photosensor 130. [ Further, the control circuit 140 can determine whether the level of the signal output from the optical sensor 130 is appropriate before analysis. If the level of the signal output from the optical sensor 130 is low, analysis can not be performed properly. Therefore, if the level of the signal output from the optical sensor 130 is lower than a predetermined reference value before the analysis, the control circuit 140 causes the light source device 110 to irradiate the sample again with light.

The light source device 110 preferably irradiates light only for a predetermined time. Since energy (power) is limited in portable devices, it is desirable to appropriately control the time for light irradiation in order to use energy efficiently. That is, it is necessary to determine what is the optimum light irradiation time for the current sample.

The time when the light source device 110 irradiates the first light is referred to as a first time. The first time may be the minimum time allowed for DNA analysis or slightly less. However, since it is difficult to know in advance the minimum time for analysis, it is preferable that the first time is about several microseconds (msec).

The control circuit 140 may control the light source device 110 to emit light for a second time longer than the first time when the level of the signal output from the optical sensor 130 is lower than the reference value. The control circuit 140 measures the level of the signal output from the optical sensor 130 after irradiating the light for the second time, and when the light is still lower than the reference value, the light source device 110 for the third time longer than the second time It is possible to control to irradiate light. The control circuit 140 may repeat this process until the level of the signal output from the optical sensor 130 becomes equal to or greater than the reference value.

The control circuit 140 can detect (quantitatively analyze) the target DNA or quantify (quantitatively analyze) the amount of the target DNA based on the signal output from the photosensor 130. The LED lamp 150 may indicate whether or not the target DNA is detected. The display panel 160 may output the result of the quantitative analysis and the target DNA detection in a predetermined text or graph format. When the display panel 160 is used, the control circuit 140 may generate and transmit a constant data signal used for the output of the display panel.

The control circuit 140 may include a chip embedding a program for the amount of the target DNA to be correlated with the amount of photons having the specific wavelength each of which is received by the plurality of photodiodes. The relationship between the amount of photons and the target DNA can be expressed through a formula that expresses a constant graph. Alternatively, a correlation table may be used to define the correlation between the amount of photons and the target DNA in advance, and quantitative analysis may be performed based on the matching table.

On the other hand, the control circuit 140 preferably performs the quantitative analysis in consideration of the amount of QD bound to the target DNA. 1, QD ₅₆₅ and QD ₆₅₅ are different from each other in the amount of QD bound to the target DNA. QD ₅₆₅ is more abundant than QD _{655 in} combination with MB. Therefore, it is necessary to analyze the emitted photons considering the difference in the amount of two QDs. For example, target DNA can be quantified based on the ratio of two QDs (QD ₆₅₅ / QD ₅₆₅ ) and the ratio of two wavelengths (660 nm / 540 nm). Further, the output signal for QD ₆₅₅ , the main signal for analysis, can be regularly normalized using the output signal for QD ₅₆₅ . This corresponds to a more accurate correction of the output signal.

Furthermore, the portable microalgae detecting apparatus 100 may include a constant optical path between the light source device 110 and the sample holder 120, and / or between the sample holder 120 and the optical sensor 130. The optical path means a path through which light is transmitted more accurately. The optical path may include a configuration such as a constant lens and a filter.

In addition, the portable microalgae detecting apparatus 100 may include a communication module 170 for transferring the result of analysis of the DNA by the control circuit 140 to another device or a network.

3 is another example of a block diagram showing the configuration of the portable microalgae detecting device 200. As shown in Fig. The portable microalgae detecting device 200 of FIG. 3 basically has a configuration corresponding to the portable microalgae detecting device 100 shown in FIG. 3, the portable microalgae detecting apparatus 200 further illustrates a circuit configuration for the portable microalgae detecting apparatus 100. As shown in FIG.

The portable microalgae detecting apparatus 200 of FIG. 3 does not include the above-described sample holder 120 as a constitution. In order to detect the green alga DNA, the sample solution should be placed in the sample holder, but the sample holder 120 is not an essential constitution in view of the circuit configuration of the portable microalgae detecting device 200. In FIG. 2, the portable microalgae detecting apparatus 200 is shown as a block, and the lower part of FIG. 2 shows an example of a circuit configuration corresponding to each structure.

The laser diode 210 irradiates the laser to a position where the sample solution is disposed. The irradiated laser is incident on the fluorescent substance (QD) of the sample solution, and the fluorescent substance emits a photon having a constant wavelength.

The emitted photons are transmitted to a photodiode 220 which senses the light of each wavelength. The photodiode 220 shows the PN photodiode. The two photodiodes 220A and 220B sense light of different wavelengths, respectively. For example, photodiodes 220A and 220B sense light at 660 nm and 540 nm wavelengths emitted from QD ₅₆₅ and QD _655, respectively. It is preferable that one photodiode 220 has a configuration of one resistor R in addition to the photodiode. This is because the intensity of the signal to be detected may be different depending on the type of the wavelength.

The portable microalgae detecting apparatus 200 preferably includes a buffer 230 connected to the photodiode 220. The buffers 230A and 230B adjust the signals output from the photodiodes 220A and 220B to a constant reference voltage. The buffer 230 controls the output signal to have a constant value by using a feedback signal.

The control circuit 250 can detect (qualitative analysis) the algae DNA or quantify (quantitatively analyze) the amount of the algae DNA based on the signal output from the buffer 230.

Furthermore, the portable microalgae detecting apparatus 200 may include an amplifier 240. The amplifiers 240A and 240B amplify the signals output from the buffers 230A and 230B to a constant level, respectively. In this case, the control circuit 250 can detect (qualitative analysis) the greenhouse DNA or quantify (quantitatively analyze) the amount of greenhouse DNA based on the signal output from the amplifier 240.

The control circuit 250 can measure the level of the signal output from the buffer 230 or the amplifier 240 and determine whether the level is appropriate for DNA analysis. If the level of the output signal is less than the reference value, the control circuit 250 can control the light irradiation time of the laser diode 210 as the light source device to be increased. The portable microalgae detecting apparatus 200 may further include a timer 215 for determining the light irradiation time of the laser diode 210. [

The initial timer 215 is set to the first time described above. The control circuit 250 can constantly increase the set time of the timer 215 when the level of the output signal is lower than the reference value and cause the laser diode 210 to irradiate the light again. The set time of the timer 215 may be repeatedly increased in the reference unit time. The timer 210 may store the final set time in a separate memory. In this case, the timer 215 holds the set time when the level of the output signal is equal to or greater than the reference value. The laser diode 210 can then illuminate the light based on the last set time stored in the timer 215. [

The display panel 290 can output the result of the quantitative analysis whether the greenhouse DNA is detected or not in the form of a constant text or graph. The control circuit 250 may process and output a constant data signal used for the output of the display panel.

The control circuit 250 may include a chip embedding a program for the amount of the target DNA to be correlated with the amount of photons having the specific wavelength each of which is received by the plurality of photodiodes. The details are the same as those of the control circuit 140 described with reference to FIG.

The communication module 270 is a configuration for transmitting the result of analyzing the DNA by the control circuit 250 to another device or a network.

Furthermore, the portable microalgae detecting apparatus 200 may include a display panel 290 capable of outputting quantitative analysis results.

The power supply unit 260 supplies power to at least one of the laser diode 210, the photodiode 220, the amplifier 240, and the display panel 290.

4 is a flowchart of a method 300 for performing DNA analysis using a portable microalgae detecting apparatus.

4 is an example of a method of detecting a target DNA using the portable microalgae detecting apparatus 100 or 200 described above. The portable DNA detecting device refers to the portable microalgae detecting device 100 or 200 described above. However, it is not limited to the green alga DNA, but is described as a specific target DNA. The detailed procedure is the same as the portable microalgae detection apparatus described above. 4 and 5 will be described with reference to the configuration of FIG.

The DNA detection method (300) using a portable DNA detection device is based on preparation of a sample solution. The process of preparing the sample solution is as described above. The sample solution assumes that QD ₆₅₅ binds to the first probe and QD ₅₆₅ binds to the second probe. Of course, various QDs that emit different wavelengths may be used.

The pretreated and post-treated sample solutions are placed (310) in a sample holder of a portable DNA detection device. The sample solution placement may allow the experimenter to place the sample container in the sample holder or to automatically introduce a certain sample into a DNA detection device placed in the same location as the stream. In the latter case, a DNA detection device is disposed in a place in contact with a river surface where a green tide may occur.

When the sample solution is placed, the laser diode irradiates the sample solution with laser light (320). A photodiode receives a photon emitted from the fluorescent material QD included in the sample solution, and emits the irradiated light (330).

Then, a certain analysis circuit determines the target DNA amount or the target DNA amount according to the output signal of the photodiode (340).

And then notifies 350 whether the target DNA is detected or / and the result of the quantitative analysis of the target DNA through an apparatus such as an LED lamp or a display device.

In some cases, the portable DNA detecting device does not directly inform the portable DNA detecting device whether the target DNA is detected and / or the quantitative analysis of the target DNA, and analyzes the result of the portable DNA detecting device to a remote server or a user terminal through a communication module Data may be transmitted. Furthermore, the portable DNA detecting device transmits the output signal of the photodiode to a server or a terminal located in a remote location in the form of packet data, and the server or terminal analyzes the data to determine whether the target DNA is detected or quantitatively analyzes the target DNA You can do it.

Control of portable DNA detection device

5 is an example of a flowchart for a method 400 for controlling a portable microalgae detecting apparatus.

First, the sample solution is placed in the portable microalgae detecting apparatus 100 or 200, and the system is initialized (410). System initialization means to delete data such as analysis results that have been performed before. Further, if there is a timer in the light source device, it may be a process of resetting the timer to the initial set time (first time). The initialization process is not an essential process.

The power supply supplies power to the laser diode, and at the same time the timer operates (420). That is, the light source device starts to irradiate the light for a predetermined time. Then, it is determined whether the timer has expired (430). If the timer has not expired (No), power is continuously supplied to the laser diode. If the timer expires (Yes), the control circuit measures the output signal level of the photodiode (440).

If the output signal level of the photodiode is below the reference value (No), the control circuit constantly increases the timer time (460). Thereafter, power is supplied to the laser diode by the increased time (420). The control circuit continuously repeats this process when the output signal level of the photodiode is lower than the reference value (No).

If the output signal level of the photodiode is equal to or greater than the reference value (Yes), the control circuit obtains the level measurement value of the output signal (470). DNA qualitative or quantitative analysis can then be performed based on the values measured by the control circuit.

On the other hand, when the laser is irradiated onto the sample for a certain period of time, the sample containing QD can emit photons for 5 to 6 minutes. In this case, the measurement of the output signal may be repeated several times for the accuracy of the measurement. For example, the control circuit may measure the level of the output signal at intervals of 30 seconds to hold a plurality of measured values. In this case, the final measurement value for the analysis can be an average value for a plurality of measurement values. Alternatively, the control circuit may use a value in the middle of the plurality of measurements to exclude the point in time at which the sample begins emitting photons (less photon generation) and the point at which the photon decreases.

In FIG. 5, the configurations in which the control circuit can measure the output signal a plurality of times are represented by 480 and 485. That is, the control circuit is initially set to a value of N = 0, and the measurement is repeated until N reaches a preset number of repetitions (No) while increasing the value of N by one. The control circuit then stores the average value of the measurements (490).

Finally, the control circuit performs DNA qualitative or quantitative analysis based on the average measurement value.

It should be noted that the present embodiment and the drawings attached hereto are only a part of the technical idea included in the above-described technology, and those skilled in the art will readily understand the technical ideas included in the above- It is to be understood that both variations and specific embodiments which can be deduced are included in the scope of the above-mentioned technical scope.

100: portable microalgae detecting device 110: light source device
120: sample holder 130: light sensor
131, 132: optical sensor 140: analysis circuit
150: LED lamp 160: Display panel
170: Communication module
200: portable microalgae detecting device 210: laser diode
215: Timer 220: Photodiode
220A, 220B: photodiode 230: buffer
230A, 230B: buffer 240: amplifier
240A, 240B: Amplifier 260: Power supply
270: Communication module 290: Display panel

Claims

The DNA detecting apparatus comprises a pre-treated DNA, a first probe connected to a first QD (quantum dot) which is a fluorescent material, a first probe coupled to a first site of the DNA, and a second probe connected to a second site of the DNA, Irradiating the sample solution including the first probe with a laser beam for a preset time of a timer;
Receiving the photodiodes of the first QD and the second QD, the photodetectors receiving the laser light, with different photodiodes;
If the output signal level of the photodiode is lower than a reference value, the DNA detecting device may increase the set time of the timer to irradiate the sample solution with the laser light secondarily; And
Analyzing the output signal of the photodiode to perform qualitative analysis or quantitative analysis of the microalgae DNA when the output signal level of the DNA detection device is equal to or greater than a reference value,
The first QD and the second QD receive the laser light and emit photons having different wavelengths respectively, and the DNA detecting device detects the output signal level of the photodiode, which receives the light of the different wavelength, The method comprising the steps of: (a)

The method according to claim 1,
The DNA detecting apparatus repeatedly irradiates the laser light while increasing the set time of the timer by the reference unit until the output signal level becomes equal to or greater than the reference value when the output signal level of the photodiode is lower than the reference value, A method for controlling a portable microalgae detecting device to be inspected.

delete

The method according to claim 1,
In the performing step, the DNA detecting device determines whether the microalgae DNA is present or quantitatively analyzes the microalgae DNA based on the amount of photons of different wavelengths emitted from the first QD and the second QD A method for controlling a portable microalgae detection device.

The method according to claim 1,
In the performing step
The DNA detection device is configured to detect the output signal level of the photodetector while the first QD and the second QD emit the photon, A method for controlling a bird detection device.

A sample holder in which a sample containing the fluorescent substance-attached DNA is placed;
A light source device for irradiating the sample with light for a set time of a timer;
A sensor device for receiving photons emitted from the sample to which the light is input; And
Wherein when the output signal level of the sensor device is lower than a reference value, the control device controls the light source device to irradiate light on the sample, increases the set time of the timer, and controls the wavelength of the photon received by the sensor device, And a control circuit for qualitative analysis or quantitative analysis of the microalgae DNA contained in the sample based on the amount of photons,
Wherein the fluorescent material includes at least two quantum dots (QD) that receive light and emit light of different wavelengths, and the control circuit controls the output signal level of the sensor device, And analyzing the microalgae DNA when the microalgae DNA is greater than or equal to the reference value.

delete

The method according to claim 6,
Wherein the sensor device comprises a plurality of photodiodes for respectively receiving photons of wavelengths emitted by the at least two QDs.

The method according to claim 6,
The control circuit
And controls the light source device to repeatedly increase the set time of the timer by a reference unit until the output signal level becomes equal to or greater than a reference value when the output signal level of the sensor device is less than a reference value, .

The method according to claim 6,
The control circuit
And performs the analysis based on an average value of output signal levels measured a plurality of times when the output signal level is equal to or greater than a reference value.