KR101666783B1 - Cosmetic composition comprising fermentates of herbal medicine fruits improving whitening and antioxidation - Google Patents

Abstract

The present invention relates to a whitening and antioxidant cosmetic composition containing a fermented oriental fruit, and it comprises seven kinds of oriental fruits such as plum, pomegranate, omija, gugija, jujube, The fermented oriental herbaceous fermented product obtained through fermentation has an antioxidative and whitening improving effect on skin cells, thereby providing an antioxidant and whitening functional cosmetic composition containing the active ingredient as an effective ingredient.

Description

TECHNICAL FIELD The present invention relates to a whitening and antioxidant cosmetic composition containing a fermented product of oriental fructose,

The present invention relates to a whitening and antioxidant cosmetic composition containing a fermented oriental fruit, and more particularly, to a cosmetic composition containing a fermented oriental fruit, And a cosmetic composition having a whitening and antioxidative function using the fermented product obtained by performing fermentation as an effective ingredient.

When the skin receives ultraviolet light, the activity of the tyrosinase enzyme in the melanocyte present in the basal layer is increased. This enzyme acts on tyrosine, a type of amino acid present in the skin tissue, to generate waveguide , Which is again converted to dopaquinone, forming melanin, which causes skin pigmentation, and the skin turns black. Therefore, for skin whitening, it is required to lower the activity of tyrosinase, which affects formation of melanin pigment. During the process of respiration, the oxygen that enters the body is used in the oxidation process, resulting in active oxygen in various metabolic processes. This active oxygen is strong oxidative and damages the cells. Especially, it is known that this active oxygen causes various diseases and aging. Recently, attention has been focused on cosmetics having antioxidative effect and development thereof is being carried out.

Most of the antioxidant ingredients for preventing skin aging are artificial chemicals because they inhibit the activity of tyrosinase to enhance whitening function for use as a functional ingredient in cosmetics. They have toxicity and stability problems when applied to long-term skin Is known. Therefore, although commercialization of natural materials having functions similar to those of conventional artificial chemicals has been continuing, satisfactory whitening and antioxidant effects have not been achieved.

Further, it is known that phenol compounds have antioxidative function in recent years, and research on this has continued. Phenol compounds inhibit the aggregation of platelets and act as antioxidants, which reduce coronary and cerebral atherosclerosis, reduce thrombosis caused by platelet aggregation in narrowing arteries, and are known to be effective in various degenerative diseases. Therefore, even in the case of a whitening composition, it is important to prepare a whitening composition under the condition that the maximum amount of the phenolic compound can be contained.

The plum, the constituent material of the present invention, entered China about 1500 years ago through China and our ancestors have been using this fruit for many years as edible or medicinal. Plum is good for the recovery of fatigue, has the effect of improving the constitution, detoxification action, inflammation relieving action, strong insecticide, sterilization action. Various organic acids and vitamins help blood circulation and make skin elastic.

The pomegranate which is a constituent material of the present invention is 5-8 m tree-grown fruit, which is cultivated in the ancient Caucasus Mountains and the Mediterranean coast, with Iran, the Himalayas and the North Indies. The pomegranate attracted attention because it turned out that middle-aged women around the Persian Gulf of origin are younger than women in other regions, and rarely experience menopausal disorders. Pomegranate contains 17mg of female hormone estrogen component per 1kg of seed, which is effective for various gynecological diseases. It increases the amount of collagen, which is good for skin beauty and has the effect of improving wrinkles.

In the pharmacological experiments, Omija, which is a constituent raw material of the present invention, has a function of stimulating central nervous system, stimulating fatigue recovery, restoring cardiovascular system function, regulating blood pressure, regulating gastric juice secretion, . The lungs and the kidneys are coughing, coughing, breathing and suffocation, and the thirst is sweating, sweating on its own, obesity, diarrhea, diarrhea, Myocardial infarction, night blindness, forgetfulness, insomnia, dermatitis, etc.

Gugija, a constituent material of the present invention, is a deciduous broad-leaved shrub belonging to Solanaceae, distributed in a temperate and subtropical region, and cultivated and nurtured in Korea, northeastern China, Taiwan and Japan have. In one room, it is used as a tonic and antipyretic. It protects liver function and improves visual acuity, prevents adult diseases such as diabetes, improves lung and kidney function, and is effective against burns. Gugija contains ingredients that promote our various physical functions and contains proteins, fats, carbohydrates, calcium, phosphorus, iron, betaine, carotene, rutin, vitamins (A, B1, B2, C) It has excellent antioxidant and anti-aging ability.

Jujube, a constituent ingredient of the present invention, has been treated as a mysterious herbal medicine or food which prevents aging in Oriental medicine. Fruit has been used empirically for nourishing agents, nervous stabilizers, blood purifiers, bark, lobster, and young leaves. There is a saying that if you do not eat jujube, you get old. This is a good basis for jujube that is effective in anti-aging. It is introduced in "Dongbibogam", "It has the power to strengthen the bowels and it is good to have common sense and help the gyeongmaek to see the tribe." In the "Chinese medicine main building", " And harmonize the nature of all kinds of medicines. " It is a fact that has been proven scientifically well.

It is known that the bokbunja as a constituent material of the present invention has an effect of encouraging energy, lightening the body and preventing gray hair from occurring. In addition, the bokbunja is an effective herbal medicine which has immunity control effect, which helps the fragile constitution, warms the body, helps the body to recover, improves the color of the skin and comforts the five eyes. Particularly useful physiologically active substances of bokbunja effectively strengthen the liver and kidney.

In the present invention, the raw material, which is a constituent raw material, is used as a medicinal material in which the fruit of a mulberry plant is dried and known as a sick person in a room. The mulberry tree is a plant that the silkworms eat and is regarded as a treasure trove of the Oriental herb, which is recorded in the legend. It is the tree of the moon which is one of the 28 constellations of the ancient Orient. I prayed here. Odie is widely used in anemia, anemia, hypertension, as well as nourishing tonic effect, which gives the blood of blood (阴血), invokes 津液, blacken gray hair and make the five days. It is also known that audi has antioxidant, anti-aging and anti-inflammatory effects.

Therefore, the inventors of the present invention have variously combined the above-mentioned seven oriental herbaceous fruits, induced the combined fermentation of L. acidophillus, L. plantarum, L. rahmnosus, and L. casei lactic acid bacteria using the extract, To prepare a skin whitening cosmetic composition.

As a prior art of the present invention, a fermentation method of natural plant material and herbal medicine, a fermented product produced by the above method, a pharmaceutical composition containing it, a cosmetic composition and a food composition are known in Korean Patent No. 10-0962587, The natural plant material is saccharified with koji and then fermented by lactic acid bacteria. In addition, Korean Patent Registration No. 10-1258646 discloses a skin eraser peeling cosmetic composition containing an ultrafine complex made by mixing and fermenting four kinds of natural products of citron, plum, pomegranate and brown rice. However, it is known that citron, plum, pomegranate, The present invention relates to a cosmetic composition for skin exfoliating and functional peel obtained by inoculating and fermenting acetobacter and lactobacillus lactobacillus strains.

Therefore, in the above patent literature, whitening and antioxidant functional cosmetic compositions containing fermented product obtained by fermenting first natural fermentation and second lactic acid fermentation by mixing raw materials of seven kinds of plum, pomegranate, omija, gugija, jujube, Compositions are not known.

Therefore, an object of the present invention is to provide a method of fermenting primary fermented raw materials and herbal lactic acid fermented raw materials mixed with raw materials of seven kinds of plum, pomegranate, omija, gugija, jujube, And a cosmetic composition containing the antioxidant.

The above object of the present invention is attained by a method for producing a herbal composition which comprises (a) 10-20% by weight of plum, 10-20% by weight of pomegranate, 10-20% by weight of Omija, 10-20% 10 to 20% by weight of bokbunja and 10 to 20% by weight of a bulkhead to prepare a herbal fruit combination; (b) washing the herbal fruit mixture obtained in the above step with water and then extracting the mixture at 121 ° C under pressure for 1 to 2 hours in a stainless steel and a flask (DSS1000, Thermos, USA); (c) Separately, wet-sterilization of the rice straw at 90 ° C for 20 minutes; (d) Rice straw sterilized in step (c) is added to the stainless steel of step (b) and the flask in an amount of 10% (v / v) Fermenting step; (e) sterilizing the first natural fermentation product obtained in step (d) at 121 ° C for 20 minutes, wet pulverizing the product, and then filtering to obtain a first natural fermentation liquid extract; (f) Lactobacillus acidophilus (KCCM41270) and Lactobacillus plantarum (Lactobacillus plantarum) mixed in the same amount (w / w) to the first natural fermentation liquid extract obtained in step (e) KCCM 11322), L. rahmnosus (KCCM11320) and L. casei (KCCM 11452) were inoculated into the stainless steel and the flask at 2 to 5 wt% A second fermentation step for a day; (g) sterilizing the fermented secondary lactic acid obtained in step (f) at 121 ° C for 20 minutes, adjusting the pH to 5, and then filtering the fermented product to prepare the herbal fermented product of the present invention, And the antioxidative and whitening improving functions of water were compared with non-fermented herbal extracts.

The present invention relates to an antioxidant and whitening agent for skin cells of an oriental fermented fruit obtained through the first natural fermentation after the first natural fermentation and the fermentation of the second lactic acid bacterium after blending the herbal raw materials of seven kinds of herbal ingredients such as plum, pomegranate, omija, But also has an excellent effect of providing an antioxidant and whitening functional cosmetic composition containing it as an active ingredient.

FIG. 1 is a diagram illustrating a manufacturing process of a fermented product according to the present invention.
2 is a graph showing the antioxidative effect of the fermented product of the oriental fruit prepared according to the present invention.
FIG. 3 is a graph showing the whitening effect of the fermented oriental fruit prepared according to the present invention.

According to the present invention,

(a) 10 to 20% by weight of plum, 10 to 20% by weight of pomegranate, 10 to 20% by weight of omiza, 10 to 20% by weight of coriander, 10 to 20% %, And 10 to 20% by weight of the bulkhead to prepare a herbal fruit combination;

(b) pressing the herbal fruit mixture obtained in the above step in a sterilized sterilized stainless steel flask (DSS1000, Thermos, USA) after washing with water at 121 ° C for 1 to 2 hours;

(c) Separately, wet-sterilization of the rice straw at 90 ° C for 20 minutes;

(d) Rice straw sterilized in step (c) is added to the stainless steel of step (b) and the flask in an amount of 10% (v / v) Natural fermentation step;

(e) sterilizing the first natural fermentation product obtained at 121 ° C for 20 minutes, wet pulverizing it, and then filtering to obtain a first natural fermentation liquid extract;

(f) Lactobacillus acidophilus (KCCM 41270), L. plantarum (KCCM 11322), Lactobacillus lambosus (Lactobacillus spp.), Inoculating 2 to 5% by weight of a mixture of L. rahmnosus, KCCM 11320 and L. casei, KCCM 11452, and fermenting the mixture for 1 to 4 days at 37 ° C;

(g) Sterilizing the fermented secondary lactic acid bacteria at 121 占 폚 for 20 minutes, adjusting the pH to 5, and filtering.

According to the present invention, wet sterilization in the step (c) is a process for removing germs other than Bacillus subtilis in rice straw, and the Bacillus subtilis is used as a starter for primary fermentation.

According to the present invention, the oriental fruity fermented product produced by the above method has antioxidant and whitening function (Experimental Examples 1 to 2) and can be used as a cosmetic composition.

The content of the fermented fruit of the present invention in the cosmetic composition is 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition containing the fermented oriental fruit according to the present invention as an active ingredient usually contains conventional auxiliary agents such as components used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, . ≪ / RTI >

Also, the cosmetic composition containing the fermented oriental fruit according to the present invention as an active ingredient may be prepared in any form conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, It can be formulated into lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation and spray, and more specifically softening longevity, nutrition lotion, nutrition cream and essence.

Hereinafter, the present invention will be described in more detail with reference to embodiments and drawings. However, the following examples are intended to illustrate the invention and are not intended to limit the scope of the invention.

Example 1. Preparation of Fermented Fruit of Oriental Fruits of the Present Invention

(W / w) of 7 varieties of oriental fruits such as plum, pomegranate, omija, gugija, jujube, bokbunja, and bokbunja were added to the pottery, and the resulting mixture was pressurized and extracted at 121 ℃ for 1-2 hours. I prepared the raw materials. Separately, wet sterilized rice straw was prepared at 90 ° C for 20 minutes for use as a primary natural fermentation starter. Next, wet sterilized rice straw was added to the fermented raw material prepared above in an amount of 10% (v / v) relative to the fermentation raw material and subjected to a first spontaneous fermentation at 37 ° C for 7 to 14 days. The first natural fermented product obtained in the above step was sterilized at 121 ° C for 20 minutes, wet pulverized with a homogenizer, and then filtered to obtain a first natural fermented liquid extract. Lactobacillus acidophilus (KCCM 41270), Lactobacillus plantarum (L. plantarum, KCCM 11322), and Lactobacillus plantarum were mixed in the same amount (w / w) A mixed culture of L. rahmnosus (KCCM 11320) and L. casei (KCCM 11452) was inoculated in an amount of 2 to 5% by weight (w / w) based on the weight of the first natural fermentation liquid extract Lactic acid bacteria were fermented at 37 ℃ for 1 ~ 4 days. After fermentation of the second lactic acid bacteria, the fermented product was sterilized at 121 ° C for 20 minutes, adjusted to pH 5, and filtered through a 150 mm filter paper to produce an oriental fermented product according to the present invention.

Comparative Example 1 Preparation of Fruit Fruit Extracts Before Fermentation

Fruits of one kind were mixed with the same amount of 7 kinds of oriental fruits such as plum, pomegranate, omija, gugija, jujube, bokbunja, and bokbunja, and they were added to the pottery for 1-2 hours at 121 ℃. .

Experimental Example 1 Antioxidative Effect of Fermented Fruit of Oriental Fruits

The antioxidative activity of the herbal fermented product obtained in Example 1 of the present invention was confirmed by fluorimetric assay using DCFH-DA (2`, 7`-dichlorofluorescin-diacetate). In the fluorimetric assay, the DCFH-DA can easily enter the cell as a nonpolar molecule. When the DCFH-DA is separated by the intracellular esterase into DCFH, which is a nonpolar polar molecule, and then oxidized by the active oxygen in the cell, dichlorofluorescin, which is measured by a fluorometer.

To evaluate this, the skin fibroblast (HDFn) was cultured in DMEM (containing 10% FBS, 1% antibiotic) at 37 ° C and 5% CO ₂ , and cells growing in the larval stage were used for the experiment. Specifically, approximately 1 x 10 ⁵ cells per well were dispensed into 96 wells, and then the fermented herbals of the present invention of Example 1 and the fermented herbal extracts of Comparative Example 1 were mixed at 10, 50 and 100 ug / mL And cultured at 37 ° C and 5% CO ₂ for 20 min. Then, 20 μM of DCFH-DA (stock 20 mM) was added to the reaction solution, and 1 mM H ₂ O ₂ was added to the reaction mixture. The reaction was carried out at 37 ° C. for 90 minutes using a spectrofluorophotometer (excitation 485 nm, emission 535 nm) Respectively. The control group (control) was used in the absence of the extract and the active oxygen species inhibition (%) was calculated by calculating the fluorescence intensity of the experimental group as a percentage of the control group. All experiments were repeated 3 times.

As shown in FIG. 2, the active oxygen species inhibitory ability was about 90% at the concentrations of 10, 50 and 100 ug / mL when the extract was added before fermentation. When the fermented product was added at a concentration of 100 ug / Respectively. From the results, it was found that the fermented fruit of the present invention is effective at a concentration of 10 ug / ml or more, but when added at a concentration of 100 ug / mL or more, antioxidation against the skin fibroblast and the whitening effect described later are excellent.

Experimental Example 2 The whitening effect of the fermented product of the present invention

The whitening effect of the fermented fruit of the present invention was confirmed by inhibiting intracellular melanin formation of mouse melanoma cells. The melanocytes are the differentiated cells in the basal layer of the epithelium of the skin. These cells have a special organs called melanocytes, which synthesize melanin. Melanin is known to play an important role not only in protecting skin from ultraviolet rays, but also as a scavenger of cytotoxic radical, as well as pigmentation such as stains and freckles. At present, in order to evaluate the efficacy of whitening raw materials, in vitro tyrosinase activity inhibition test and intracellular melanin formation inhibition test were most commonly performed.

To confirm this, mouse melanoma cells (B-16 F1) were cultured in DMEM (supplemented with 10% FBS, 1% antibiotic) at 37 ° C and 5% CO ₂ conditions and cells growing in the larval stage were used in the experiment . Specifically, about 1 x 10 ⁵ cells per well are dispensed into 6 wells and cultured to about 80% confluency. The pre-fermentation Oriental fruit extract in comparison to an existing medium of each well after incubation and Oriental fruit fermented product of Example 1, the present invention Example 1 10, 50, haejugo replaced with 100 ug / mL was added medium 37 ℃, CO ₂ 5 % ≪ / RTI > conditions for 24 hours. Then, the cells were treated with trypsin, recovered, and the same number of cells were transferred to a tube using a hematocytometer and centrifuged to obtain a cell pellet. The cell lysis buffer (Thermo scientific) was added to the pellet and incubated in a 60 ° C incubator for 30 minutes to obtain intracellular melanin. The melanin solution obtained in the above step was measured for OD (OD) at a wavelength of 405 nm using a microplate meter. The control group (control) was not added with the above extract, and the inhibitory effect on intracellular melanin synthesis was quantified by calculating the absorbance of the experimental group relative to the control group in%. All experiments were repeated 3 times.

As shown in FIG. 3, the intracellular melanin inhibitory effect was about 87% at the concentration of 100 ug / mL before the fermentation and about 72% at the time of the addition of the herbal fermented product of the present invention to the melanin inhibitory effect of about 15% Respectively.

Hereinafter, a preferred formulation example is provided to facilitate understanding of the present invention. However, the following formulation examples are provided only for a better understanding of the present invention, and the present invention is not limited by the formulation examples. The fermented product prepared in Example 1 was used for the oriental fermented product of the following formulation example.

Formulation Example 1. Softening longevity

The softening longevity of the oriental fermented fruit produced according to the present invention as an active ingredient was prepared as shown in Table 2 below.

ingredient Content (% by weight) The fermented product of the present invention (Example 1) 1.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Polyoxyethylene hardened castor oil 1.0 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Formulation Example 2. Nutrition Lotion

Nutritional lotions containing the oriental fermented fruit produced according to the present invention as an active ingredient were prepared as shown in Table 3 below.

ingredient Content (% by weight) The fermented product of the present invention (Example 1) 1.0 Wax 1.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Sorbitan stearate 1.0 Pro-type glycerin monostearate 0.5 Stearic acid 1.5 Glyceryl stearate / FG-400 stearate 1.0 Propylene glycol 3.0 Carboxy polymer 0.1 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Formulation Example 3. Nourishing cream

The nutritional cream containing the fermented oriental fruit prepared according to the present invention as an active ingredient was prepared as shown in Table 4 below.

ingredient Content (% by weight) The fermented product of the present invention (Example 1) 1.0 Stearic acid 2.0 Cetyl alcohol 2.0 Glyceryl monostearate 2.0 Polyoxyethylene sorbitan monostearate 0.5 Sorbitan sesquioleate 0.5 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 Wax 1.0 Liquid paraffin 4.0 Skallah 4.0 Caprylic / capric triglyceride 4.0 Carboxyvinyl polymer 0.3 Butylene glycol 5.0 glycerin 3.0 Triethanolamine 0.5 Purified water Balance

Formulation Example 4. essence

Essences containing the fermented product of oriental fruity according to the present invention as an active ingredient were prepared as shown in Table 5 below.

ingredient Content (% by weight) The fermented product of the present invention (Example 1) 1.0 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceramide 0.7 Steareth-4 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Macadamia oil 15.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.2 antiseptic a very small amount Spices a very small amount Purified water Balance

As described above, the present invention provides a fermented herbal fermented product obtained by mixing 7 types of fruits such as plum, pomegranate, omija, gugija, jujube, bokbunja, and bokbunja, followed by primary natural fermentation and secondary lactic acid fermentation, The cosmetic composition is an extremely useful invention in the cosmetics industry because it has an excellent antioxidant and whitening function.

Claims (5)

(a) 10 to 20% by weight of plum, 10 to 20% by weight of pomegranate, 10 to 20% by weight of omiza, 10 to 20% by weight of coriander, 10 to 20% %, And 10 to 20% by weight of the bulkhead to prepare a herbal fruit combination; (b) adding the herbal fruit mixture obtained in the step (a) to the pottery which has been heated and sterilized, followed by pressure extraction at 121 ° C for 1 to 2 hours; (c) Separately, wet-sterilization of the rice straw at 90 ° C for 20 minutes; (d) Rice straw sterilized in step (c) is added to the pottery of step (b) at a concentration of 10% (v / v) relative to the volume of the herbal mixture and subjected to primary fermentation for 7 to 14 days at 37 ° C step; (e) sterilizing the first natural fermentation product obtained in (d) at 121 ° C for 20 minutes, wet pulverizing the product, and then filtering to obtain a first natural fermentation liquid extract; (f) Lactobacillus acidophilus, L. plantarum, and Lactobacillus lambatus, which are mixed in the same amount (w / w) with the liquid extract obtained in (e) above. 2 to 5% by weight of a mixture of L. casein and L. casei, and then fermenting for 1 to 4 days at a temperature of 37 DEG C; (g) sterilizing the fermented secondary lactic acid bacteria at 121 占 폚 for 20 minutes, adjusting the pH to 5, and then filtering the resulting fermented product. The method for producing an antioxidant and whitening functional herbal fermentation product
The antioxidant and whitening functional oriental herbaceous fermentation product prepared by the method of claim 1 An antioxidant and whitening functional cosmetic composition containing the fermented product of the oriental fruit of claim 2 as an active ingredient The cosmetic composition according to claim 3, wherein the effective amount of the fermented oriental fruit is 10 ug / mL to 1 g / mL, The cosmetic composition according to claim 3 or 4, wherein the cosmetic composition is a formulation of any one of softening water, nutritional lotion, nutritional cream, and essence

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