KR101460669B1 - Cosmetic composition with mucus from fish - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mucus obtained from a mucus-secreting fish. The cosmetic composition of the present invention is useful as an antioxidant, promoting collagen synthesis, improving skin wrinkles, whitening, moisturizing, restoring skin barrier function, Excellent anti-inflammatory effect.

Description

The present invention relates to a cosmetic composition containing mucus obtained from a fish which secretes mucus,

The present invention relates to a cosmetic composition containing a slime obtained from a fish that secretes mucus.

Skin aging can be divided into two types: intrinsic (chronological aging) and phtoaging (Gilchrest BA: J. Am . Acad . Dermatol ., 21, 610-613 (1989)]. Endogenous aging is a naturally occurring aging phenomenon that is accompanied by decreased physiological function of the body as age increases [Braverman IM et al . : J. Invest. Dermatol ., 78, 434-443 (1982)]. Photoaging means that the skin is repeatedly exposed to light to change the appearance or function of the skin [Ridder GM et al . : J. Am . Acad . Dermatol ., 25, 751-760 (1991)]. In addition to the above, skin aging can be caused by active oxygen species activated by ultraviolet rays, stress, disease states, environmental factors, wounds, and aging. When such conditions are deepened, the antioxidant defense network existing in the living body is destroyed, And tissues to promote adult diseases and aging. More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins causes severe hyperinflammation of collagen, hyaluronic acid, elastin, proteoglycans, and fibronectin, which are connective tissues of the skin, which interferes with skin elasticity. Mutation, cancer induction, and immune function.

Therefore, it is necessary to protect the cell membranes by abolishing reactive oxygen species that are mediated by the body's metabolic processes, ultraviolet irradiation, and inflammatory reactions, and regenerate already damaged cells by active metabolism to proliferate the cells Can be restored and healthy skin can be maintained. In aging, not only reactive oxygen species but also MMP (Matrix metallopretease) enzyme is involved. Synthesis and degradation of extracellular matrix such as collagen are controlled appropriately in vivo, but its synthesis is decreased as aging progresses and enzymes degrading collagen (MMP) is promoted and the elasticity of the skin is lowered and wrinkles are formed. In addition, these degrading enzymes are activated by ultraviolet exposure. Therefore, there is a demand for development of a substance capable of controlling the activation of MMP induced in the cell by ultraviolet light or inhibiting its activity. However, the raw materials used as cosmetic materials have mostly suppressed only the activity of degrading enzyme (MMP).

On the other hand, melanin is converted from tyrosine to dopa and dopaquinone by the action of tyrosinase existing in pigment cells, and is generated by dopachrome. Melanin is present in the skin, protects the body from ultraviolet light and has an important function in regulating hormone secretion in the body. However, when melanin is overproduced, it is known that it forms spots and freckles, promotes skin aging, and plays an important role in skin cancer induction, and research and development for preventing melanin overproduction has been actively carried out. (Japanese Patent Laid-open No. 4-93050) and plant extracts inhibit tyrosinase (Japanese Patent Laid-open Publication No. 4-9320), hydroquinone (Japanese Patent Laid Open No. 6-192062), kojic acid And is used as a whitening cosmetic. However, these components are poor in stability among cosmetic formulations, they are decomposed to cause coloration, odor generation, efficacy at a living body level, effects are unclear, or safety is a problem, and their use is limited.

On the other hand, in the human body, the skin is an organ acting as a protective film for protecting the living body from the external environment. It prevents loss of biological components in the human body, that is, water and electrolytes, and prevents harmful substances from entering the human body from the outside . The skin is divided into the skin layer, the dermal layer and the subcutaneous fat layer. The epidermal layer consists of keratinocytes and melanocytes. Since the keratinocytes in the outermost layer of the skin are in direct contact with the external environment, they are required to have strong resistance against physical, chemical, or material permeation and prevent moisture from being lost to the outside At the same time as zooming, it should maintain its flexibility by holding proper water on its own.

Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer, which is about 10 to 30% in the keratin layer. The water supplied from the dermis layer mainly migrates to the upper part of the stratum corneum by passive diffusion and eventually to the outside. This is called Trans Epidermal Water Loss (TEWL). It is known that the lipid component of the keratinocyte layer and epidermal lipid, which maintains the transdermal water loss at an appropriate level, is the lipid component.

On the other hand, it is known that the keratinocyte layer has a hydrophilic water retention ability called a natural moisturizing factor (NMF) and plays an important role in moisturizing the skin. Normal keratinocyte layer maintains skin smoothness and softness and maintains normal body protection function when moisture is maintained at about 10 to 30%. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the protective function of the body is lost and the aging phenomenon occurs. For example, in the case of dry skin, the keratinocyte aggregation is weakened, and a scaling phenomenon appears in which the keratinocytes are peeled off like scales from the skin surface. The reason why the skin is dried in this way is that the moisture content of keratinocyte layer is smaller than that of normal skin. In addition to this, even healthy skin may be in a harsh environment, such as wind, cold weather, sunshine, cleansing, shaving, etc., which causes water shortage, which can lead to poor skin condition.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer. To this end, cosmetics were added with ingredients similar to sebum, moisturizers such as NMF or polyol, and the like. For example, glycerin, solitol and the like having three or more hydroxyl groups (OH groups) as water-soluble polyols exhibit excellent moisture resistance, but they are very sticky and feel uncomfortable when used. Propylene glycol having two hydroxyl groups (OH groups) 3-butylene glycol, etc. may cause side effects of the skin. In addition, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic, which deteriorates emulsion stability of the cosmetic. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize.

The ability of the horny layer to retain moisture can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. And the development of a substance with high moisturizing effect is one of the important research subjects in cosmetics.

Meanwhile, in recent years, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased.

The present inventors have studied the applicability of various natural products to cosmetics. As a result, they have found that mucus obtained from fishes having mucus-secreting epidermis is selected from these mucilages and has antioxidants, promotes collagen synthesis, improves skin wrinkles, , Skin barrier function recovery, atopy improvement effect, and anti-inflammatory effect, and completed the present invention.

The present invention has been developed as a cosmetic composition having antioxidant activity, promotion of collagen synthesis, improvement of skin wrinkles, whitening, moisturizing, restoration of skin barrier function, anti-inflammatory effect and atopy improvement by obtaining mucus from fish having epidermis secreting mucus The purpose is to provide.

In order to achieve the above object, the present invention provides a cosmetic composition characterized by containing mucilage obtained from a fish having mucus epidermis secreting mucus to the skin as an active ingredient.

In the present invention, a fish having a so-called 'mucous epidermis' that secretes mucus into the skin is a fish which has no scales (catfish) and is covered with a mucous substance, Includes fishes with deeply buried round scales and the epidermis enclosed in mucous material. Examples include sea eel, eel, loach, mudfish, catfish, and horseshoe. For the convenience of work, it is better to use eel and eel.

On the other hand, the cosmetic composition of the present invention is characterized by containing mucilage obtained from a fish having mucus epidermis as an active ingredient. The amount of mucus added in the composition is not limited to a specific range, but preferably, By weight based on the total weight of the composition. If the mucus is contained in the above range, the effect of the mucilage obtained from the fish having the mucous epidermis can be fully exerted without deteriorating the quality suitability of the cosmetic composition.

On the other hand, the cosmetic composition of the present invention may be used, for example, as any one of antioxidant, wrinkle, skin whitening, atopic dermatitis, skin moisturizing, skin barrier function recovery, .

On the other hand, the cosmetic composition according to the present invention may contain, besides the slime obtained from the fish having the mucous epidermis, other ingredients capable of synergizing with the main effect within a range not impairing the main effect of the present invention It is also possible to do.

Meanwhile, the composition according to the present invention can be used as an adjuvant commonly used in cosmetics such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, perfumes, fillers, ≪ / RTI > The amounts of these various adjuvants are those conventionally used in the art, for example, 0.001 to 30% by weight based on the total weight of the composition. However, in any case, the adjuvant and its proportion will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

Meanwhile, the formulations of the cosmetic composition of the present invention are not limited to specific ones, and examples thereof include emulsions of lotion, gel, water-soluble liquid, cream, essence, oil-in-water type and w / A color cosmetic formulation consisting of an essential cosmetic formulation made of ointment, an underwater type and a water-in-oil type make-up base, a foundation, a skin cover, a lipstick, a lip gloss, a face powder, a two way cake, an eye shadow, a teak color and an eyebrow pencil One is good. It may also optionally be applied to the skin in aerosol form, in solid form, e.g. in the form of a stick, and as a skin care and / or makeup product.

The cosmetic composition of the present invention is excellent in antioxidation, promoting collagen synthesis, improving skin wrinkles, whitening, moisturizing, restoring skin barrier function, improving atopy and anti-inflammatory effect.

Further, since the present invention uses fish mucus, it is possible to provide a cosmetic composition which is harmless to human body and is excellent in safety.

Hereinafter, the present invention will be described in more detail in the following examples. However, the scope of the present invention is not limited to the following examples, and all the equivalent technical ideas are included.

Example  : Obtain mucus from fish with mucoid epidermis

Eucalyptus and eelgrass of about 50 ~ 60 ㎝, which can be obtained commercially, were selected as the mucous fishes and they were raised by the general method. Eel and eel were washed in clean running water and selected for similar size (50 ~ 60 cm), cleaned with 70% ethanol and weighed to obtain accurate mucilage.

The eels were weighed and ewes were weighed for 10 minutes. After feeding for 12 hours, the mucilage was recovered in the same manner as above. The collected mucilage was stored in the refrigerator.

Test Example 1: Measurement of antioxidative effect using NBT method

In this test example, the antioxidative activity of retinol and BHT was compared with the antioxidant such as retinoic acid and BHT in a laboratory to determine the antioxidative effect of the eel and the moxa obtained in the examples.

To measure the antioxidant effect, active oxygen produced by xanthine and xanthine oxidase is measured by the NBT method and the effect of the test substance on the removal of active oxygen, that is, the effect of active oxygen scavenging is evaluated. The active oxygen scavenging rate is measured by measuring the blue color produced by the reaction of active oxygen with Nitro Blue Tetrazolium (NBT) at a wavelength of 560 nm by generating active oxygen by xanthine and xanthine oxidase .

As a measuring method,

1. 0.05M Na2CO3 ------------------------------------ 2.4ml

2. 3 mM xanthine solution - 0.1 ml

3. 3 mM EDTA solution ------------------------------------ 0.1 ml

4. BSA solution ----------------------------------------- 0.1ml

5. 0.72 mM NBT solution - 0.1 ml

6. Xanthine oxidase solution - 0.1 ml

7. 6 mM CuCl2 solution - 0.1 ml

① Add 1, 2, 3, 4, and 5 to Bayer's disease, add 0.1 ml of sample solution, and leave at 25 ℃ for 10 minutes.

② Add 6, stir rapidly, and start culturing at 25 ℃ for 20 minutes.

③ Then, 7 is added to stop the reaction, and the absorbance St at 560 nm is measured.

(4) The absorbance Bt is measured by using distilled water instead of the sample solution.

⑤ Blank of the sample solution should be treated by distilled water instead of 6 to measure the absorbance Bo.

The result of the effect was calculated by Equation (1), and the results are shown in Table 1.

[Equation 1]

Inhibition rate (%) = [1- (St-So) / (Bt-Bo)] X 100

St: Absorbance at 560 nm after enzyme reaction of sample solution

Bt: Absorbance at 560 nm after enzyme reaction of blank test solution

So: the absorbance at 560 nm before the reaction in the absence of enzyme in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of enzyme in the blank test solution

As shown in Table 1, excellent antioxidative effects were observed in all of the samples tested at the treatment concentration of 0.1%, and the antioxidative effects of eel and eel mushroom were 88 and 90%, respectively, indicating excellent antioxidative effects similar to those of retinol and BHT .

Name of sample Treatment concentration (%) Antioxidative effect(%) Eel mucus 0.1 88 Mite mucus 0.1 90 Retinol 0.1 88 BHT 0.1 84

Test Example  2: DPPH Experiment on antioxidant effect test

In order to measure the antioxidant activity of the eel and moxa mushroom obtained in the Examples, this test example was conducted by using the DPPH method with antioxidants such as green tea extract and vitamin E as a comparative sample under laboratory conditions.

The DPPH method measures antioxidative activity by reducing power using a free radical called 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (DPPH). The degree of reduction of the absorbance by reduction of DPPH by the test substance is compared with the absorbance of the blank test solution and the free radical scavenging ratio is measured at a wavelength of 560 nm.

As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chemical Co., MW = 618.76) was dissolved in methanol to make 100 ml.

As a measuring method,

(1) Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and start culturing at 25 ° C for 10 minutes.

② Measure the absorbance St at 560 nm thereafter.

(3) The absorbance Bt is measured by the same procedure as above except that distilled water is used instead of the sample solution.

(4) Blank of sample solution is measured by the same procedure using methanol instead of 0.1 mM DPPH solution.

The result of the effect was calculated by Equation (2), and the results are shown in Table 2.

&Quot; (2) "

Inhibition rate (%) = [1- (St-So) / (Bt-Bo)] X 100

St: absorbance at 560 nm after free radical scavenging of the sample solution

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of free radicals in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

As shown in Table 2, eel and eel mucus had better antioxidative effect than green tea extract and vitamin E at the concentration of 0.01%.

Name of sample Treatment concentration (%) Antioxidative effect (%) Eel mucus 0.01 58 Mite mucus 0.01 61 Green tea extract 0.01 40 Vitamin E 0.01 37

Test Example  3: in vitro MMP -One inhibition assay

This test example measures the inhibitory activity of substrate metalloproteinases (MMP-1) in a biochemical model in order to measure the anti-aging effect of the eel and eel mucus obtained in the examples. The purified collagenase and its substrate (EnzChek® gelatinase / collagenase kit, Molecular Probes), based on the use of collagen and gelatin conjugated to fluorescein.

The collagenase purified from Clostridium histiochem was supplied into the EnzChek (TM) gelatinase / collagenase kit. The reaction buffer consisting of DQ-collagen purified from porcine skin and conjugated to fluorescein and 0.05 M Tris-HCl, 0.15 M NaCl, 5 mM CaCl2, and 0.2 mM sodium azide (pH 7.6) was diluted with EnzChek gelatinase / Collagenase kit (Molecular Probes) was used. Eel and eel mucus were dissolved in the reaction buffer. This is 4; 2; 0.4; 0.2; 0.1% (w / v). The dilution of the test extract was incubated with DQ-collagen at 25 ug / ml and 0.1 U / ml collagenase for 15, 45 and 120 minutes at room temperature.

In each experimental condition, the control mixture corresponding to the collagenase and DQ-collagen mixture was similarly incubated.

Also, in each experimental condition, a sample called Blank, hereinafter referred to as an " enzyme-free blank " was incubated in the presence of DQ-collagen and in the absence of collagenase. Each experiment was performed three times.

After 15 minutes, 45 minutes and 120 minutes, the signal corresponding to the decomposition of DQ-collagen was measured with a fluorescence analyzer (excitation: 485 nm, emission 505 nm).

The fluorescence value of each sample was measured based on the 'enzyme-free blank' fluorescence value. The results were expressed as fluorescence units per sample and percent change relative to the control.

In conclusion, eel and eel mucus tested at 0.04 to 4% (v / v) under selected experimental conditions possessed anti-collagenase / gelatinase activity in a dose-dependent manner.

As a result, as shown in Table 3, it was confirmed that 83.4% of the collagenolytic activity of clostridium collagenase was inhibited by 0.04% of eel and eel mucus, which is superior to the inhibitory effect of green tea extract.

Experimental Sample Inhibition rate (%) Treatment concentration (%) 0.02 Treatment concentration (%) 0.04 Eel mucus 68 85 Mite mucus 67 83 Retinol 0 9 Green tea extract 55 70

Test Example  4: Euphorbia and Euphorbia after UV irradiation MMP -1 expression inhibition evaluation

In this test example, ELISA was performed to measure the concentration of MMP-1 after UV irradiation and sample addition of eel and eel mucus obtained in the examples.

UVA is irradiated to human dermal fibroblasts at an energy of 5 J / cm < 2 > using a UV chamber. Ultraviolet irradiation dose and incubation time were established by preliminary experiment to maximize MMP expression level in fibroblasts. Negative controls were wrapped in silver foil and kept in the UVA environment for the same amount of time. UVA emission was measured using a UV radiometer. During UVA irradiation, cells were kept in the previously dispensed medium, irradiated with UVA, exchanged with the medium containing the sample, cultured for 24 hours, and the medium was recovered and coated on a 96-well plate. The primary antibody (MMP-1 (Ab-5) monoclonal antibody and MMP-2 (Ab-3) monoclonal antibody) was treated and reacted at 37 ° C for 60 minutes. After incubation for 60 minutes with a secondary antibody, anti mouse IgG (alkaline phosphatase conjugated), the reaction was carried out at room temperature for 30 minutes with an alkaline phosphatase substrate solution (1 mg / ml ρ-nitrophenyl phosphate in diethanolamine buffer) Absorbance at 405 nm. As a control group, a sample to which no sample is added is used.

The results are shown in Table 4. As shown in Table 4, the inhibition rate of MMP-1 induced by UV irradiation was 20% or more, compared to the control group without treatment, Which is superior to the inhibition rate.

Test group Treatment concentration (%) MMP-1 expression inhibition rate (%) Control group - - Eel mucus 0.1 25 Mite mucus 0.1 24 Retinol 0.1 21

Test Example  5: B16F1 Melanocyte  Experiment to measure the inhibitory effect of melanin formation

The present example is to determine the whitening effect of B16F1 melanocyte by checking the degree of inhibition of melanin formation in order to confirm the whitening effect of the eel and mite mucus obtained in the examples.

The B16F1 melanocyte used in this example is a cell strain derived from a mouse, and is a cell that secretes a melanin pigment called melanin. During the artificial culture of these cells, samples were treated to compare the degree of reduction of melanin pigment. The B16F1 melanocyte used in this example was purchased from ATCC (American Type Culture Collection, Accession No. 6323).

The melanin biosynthesis inhibitory effect of B16F1 melanocyte was measured as follows. B16F1 melanocyte was dispensed in a 6-well plate at a concentration of 2 x 10 ⁶ cells per well and the cells were incubated for 72 hours at a concentration that did not induce toxicity. After incubation for 72 hours, cells were detached with trypsin-EDTA, and the number of cells was measured and centrifuged to recover the cells.

Quantification of intracellular melanin was carried out using Lotan: Cancer Res . , 40: 3345-3350, 1980). Cell pellet was washed once with PBS, and 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin, and the absorbance of melanin was measured at 405 nm with a microplate reader. The inhibition rate (%) of melanin formation was measured. Melanogenesis inhibition rate (%) of B16F1 melanoma site was calculated by the equation 3, IC ₅₀ value is the concentration of a substance that inhibits melanin production by 50%.

&Quot; (3) "

Inhibition rate (%) = [(A-B) / A] x 100

A: Amount of melanin in wells to which no sample was added

B: Amount of melanin in the well to which the sample was added

As shown in Table 5, the IC ₅₀ values of eel and eel mucus were 0.10% and 0.09%, respectively, and the conventional whitening agents hydroquinone, arbutin, oil soluble licorice extract, The results are similar or superior to those of the conventional method.

Name of sample Melanin formation inhibitory effect (IC ₅₀ ) Eel mucus 0.10% Mite mucus 0.09% Hydroquinone 0.06% Arbutin 0.31% Eucalyptus extract 0.12% Usefulness Licorice extract 0.04%

Test Example  6: Inhibitory effect of inflammatory cytokine expression by ultraviolet irradiation

This test example evaluates the degree of suppression of inflammatory cytokine expression expressed by ultraviolet irradiation to confirm the anti-inflammatory effect of the eel and eel mucus obtained in the examples.

Fibroblasts isolated from human epidermal tissue were placed in a 24-well test plate in an amount of ⁵ × 10 ⁴ and adhered for 24 hours. Each well was washed once with PBS and 500 占 퐇 of PBS was added to each well. After irradiating the fibroblasts with 10 mJ / cm 2 of ultraviolet light using an ultraviolet B (UVB) lamp (Model: F15T8, UVB 15W, Sankyo Dennki, Japan), PBS was removed and the cells were cultured in DMEM Medium) was added. The eel and eel mucus to be evaluated were treated and cultured for 5 hours. The inhibitory effect of IL-1α on the expression of inflammatory cytokines in the eel and eel mucus was determined by taking 150 μl of the culture supernatant. The amount of IL-1α was quantitated using an enzyme-linked immunosorbent assay (ELISA), and the production rate of IL-1α was calculated by Equation (4).

&Quot; (4) "

Inhibitory rate of inflammatory cytokine expression (%) = [1- (St-Bo) / (Bt-Bo)] X 100

Bo: IL-1? Production in wells without UV irradiation

Bt: IL-1? Production in wells irradiated with ultraviolet light and not treated with the sample

St: IL-1α production in wells irradiated with ultraviolet light and treated with the sample

As shown in Table 6, eel and eel mucus inhibited the production of IL-1α, which is an inflammatory cytokine caused by ultraviolet light, by 67% and 65%, respectively, at a concentration of 0.02% I could see the defensive.

Name of sample Treatment concentration (%) Inhibitory rate of inflammatory cytokine expression (%) Eel mucus 0.01 35 0.02 67 Mite mucus 0.01 34 0.02 65

Test Example  7: Skin whitening  Measurement experiment

This test example was prepared by preparing a cosmetic preparation containing the eel mucus obtained in the examples, and evaluating the skin whitening effect in humans by performing a comparative experiment with Comparative Example 1.

The cosmetic composition used in the comparative experiment was in the form of a cream, and its composition was as shown in Table 7. First, the b) image recorded in Table 7 was heated and stored at 70 ° C. A) was added to the mixture, followed by preliminary emulsification, homogeneously emulsified with a homomixer, and then gradually cooled to prepare a cream (Example 1, Comparative Example 1). The cream prepared in Example 1 was applied to the right side of the face and the cream prepared in Comparative Example 1 was applied to the left side of the face for 2 consecutive months for 10 consecutive subjects (10 to 20 years old female) . After completion of the test, the skin of the applied area on both right and left sides of the face was analyzed by image analyzer and color change (ΔL) of the color was measured using a Minolta CR300, and an objective visual observation by a plurality of expert Subjective visual observation was performed and the effect was measured according to the following classification. The results are shown in Table 8 below. At this time, the degree of whitening efficacy was classified into the following seven grades and evaluated.

[Evaluation criteria for whitening efficacy]

-3: very bad, -2: worse, -1: slightly worse, 0: no change, 1: slight improvement, 2:

As shown in Table 8, it was found that the whitening effect was excellent in the facial skin of an experimenter who applied cream containing eel mucus.

Raw material Example 1 Comparative Example 1 end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic acid cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mole addition) alcohol ester 3 3 Glyceryl monostearate Aster 2 2 Liquid paraffin 5 5 I Eel mucus 5 - Propylene glycol 5 5 glycerin 4 4 Purified water to 100 to 100

Note) Unit: wt%

Skin-colored
The brightness change (? L) Objective evaluation of expert Subjective evaluation of the subject Example 1 Comparative Example 1 Example 1 Comparative Example 1 medium 3.4 2.7 1.7 2.9 1.8

ΔL = skin brightness after test - skin brightness before test

Test Example  8: Skin wrinkles  Improvement effect measurement experiment

In this test example, a cosmetic preparation containing the eel mucus obtained in the Examples was prepared, and the skin wrinkle-improving effect and the skin-cosmetic effect were evaluated in humans. The cosmetic composition used in the comparative experiment was in the form of a cream, and its composition was as shown in Table 7. The cream prepared in Example 1 and Comparative Example 1 was applied to 10 subjects (20 to 35 year-old female), the cream prepared in Example 1 on the right side of the face, the cream prepared in Comparative Example 1 on the left side of the face Were applied twice a day for two consecutive months. After the completion of the experiment, the effect of alleviating the skin wrinkles was measured by using a silicone resin replica (replica) before and after use for 2 months, and the wrinkles around the eyes were compared with the skin wrinkles and skin image analyzer.

Table 9 compares the average fine wrinkle depth and wrinkle reduction ratio of the eye of the experimenter using the cream prepared in Example 1 with the creamer of Comparative Example 1. As shown in Table 9, it was found that the skin wrinkle improving effect was excellent in the skin around the eyes of the subjects who applied the cream containing the eel mucus.

Example 1 Comparative Example 1 Before use After 2 months use Before use After 2 months use Average fine wrinkle depth 0.190 0.156 0.188 0.181 Wrinkle reduction rate before use (%) 17.89% 3.72%

n = 10, p < 0.05

Test Example  9: skin moisturizing effect measurement experiment

This test example was prepared by preparing cosmetic preparations containing the eel mucus obtained in the examples and evaluating skin moisturizing effects in humans by performing comparative experiments with Comparative Examples 2 to 5.

The cosmetic composition used in the comparative experiment was in the form of a cream, and the composition thereof was as shown in Table 10. First, the b) image recorded in Table 10 is heated and stored at 70 ° C. A) was added to the mixture, followed by preliminary emulsification, uniformly emulsified with a homomixer, and then gradually cooled to prepare a cream (Example 2, Comparative Examples 2 to 5). Twenty subjects of the experiment were applied to the lower part of the Example 2 and Comparative Examples 2 and 5, and about 90 minutes later, the humidifying ability before and after the application was evaluated by a Corneometer (CM 820).

Raw material Example 4 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 end Stearyl alcohol 8 8 8 8 8 Stearic acid 2 2 2 2 2 Stearic acid cholesterol 2 2 2 2 2 Squalane 4 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 6 Polyoxyethylene (25 mole addition)
Alcohol ester 3 3 3 3 3 Glyceryl monostearate
Aster 2 2 2 2 2 I Eel mucus 5 - - - - glycerin - - 5 - - Na-hyaluronate - - - 5 - 1,3-butylene glycol - - - - 5 Purified water to 100 to 100 to 100 to 100 to 100

Note) Unit: wt%

division Example 2 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Type of moisturizer Eel mucus - glycerin Na-hyaluronate 1,3-butylene glycol Corneometer
(△ Hydration) 18 5 10 13 9

As a result of the test, as shown in Table 11, it was found that the moisturizing effect was excellent in the facial skin of an experimenter who applied cream containing eel mucus.

Test Example  10: Percutaneous water loss ( TEWL  : Trans - epidermal water loss ) Measure

This test example was conducted by preparing a cosmetic composition containing the eel mucus obtained in the examples and using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) for trans-epidemic water loss (TEWL) And a comparative experiment was conducted. The cosmetic composition used in the comparative experiment was in the form of a cream, and the composition thereof was as shown in Table 10 above.

Each sample was applied to the right and left faces of 40 women in their 20s and 40s. Percutaneous water loss before, 1, 2, 4, and 6 hours was applied before and 3 cm from the eye. The results are shown in Table 1. The transdermal water loss was measured using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) three times and the mean value was evaluated.

division 1 hours 2 hours 4 hours 6 hours Example 2 14.7 11.5 9.6 7.2 Comparative Example 2 18.6 16.7 13.5 10.8 Comparative Example 3 16.8 14.1 12.2 9.5 Comparative Example 4 15.4 14.6 11.9 9.7 Comparative Example 5 15.5 13.8 11.4 9.2

As shown in Table 12, the amount of transdermal water loss was measured. As a result, it was found that the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied cream containing eel mucus.

Test Example  11: atopy improvement effect

This test example was evaluated by comparing the cosmetic compositions of Example 1 and Comparative Example 1 with those of atopic individuals.

Twenty subjects (20 to 40 years old) with atopic skin were examined for the cream prepared in Example 1 for atopic skin area and the cream prepared in Comparative Example 1 for the remaining 10 subjects twice per day And applied continuously for 4 weeks. Atopic dermatitis severity, IgE change, coral leucocyte (Eosinophil) change, moisture content, and skin acidity were measured before and 4 weeks after product application (0 week). There were no adverse events in all subjects participating in the 4-week study.

Table 13 shows the comparison of the effect of improving the atopy of the experimenter using the cream prepared in Example 1 and Comparative Example 1.

Name of sample Atopic dermatitis severity IgE change Coral leukocyte change Moisture content Skin acidity I'm after I'm after I'm after I'm after I'm after Example 1 37.50 29.21 434.1 318.6 314 247 25.85 26.90 5.723 6.068 Comparative Example 1 37.76 33.05 446.8 366.4 320 283 26.35 25.00 5.711 5.825

n = 20, p < 0.05

As shown in Table 13, it was found that in an experiment in which an atopic dermatitis severity, a change in blood IgE and coral leukocyte, a moisture content, and a skin acidity, which are diagnostic criteria of atopic skin, , It was found that the eel mucus of the present invention is excellent in atopy improvement effect.

Claims (10)

A mucilage obtained from a fish having mucus epidermis that secretes mucus into the skin as an active ingredient,
Wherein the fish is any one selected from sea eel, eel, eel, and catfish.
delete The method according to claim 1,
The mucus-
Wherein the cosmetic composition is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the composition.
The method according to claim 1,
In the cosmetic composition,
Wherein the composition is an antioxidant.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for skin whitening.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for improving skin wrinkles.
The method according to claim 1,
In the cosmetic composition,
Wherein the composition is for moisturizing the skin.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for improving atopy.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for improving the skin barrier function.
The method according to claim 1,
In the cosmetic composition,
Lt; RTI ID = 0.0 > anti-inflammatory < / RTI >