KR101409325B1 - Apparatus for multiple reaction and purification of chemical - Google Patents

Abstract

The present invention relates to a device for multi-reaction and purification of a chemical compound, wherein a cartridge unit consisting of a plurality of columns can be simply fixated because the cartridge unit is covered and fixated by means of a top mat and a cover through a same level pressure when a chemical compound is reacted; and a fixing groove is formed on the top of a purifying unit so that a compound of the cartridge unit can be injected in a purifying cartridge at once. The device comprises: a tray whereby a cartridge unit consisting of a plurality of columns to store a chemical compound is formed and a plurality of support plates are formed on a top direction of the outside thereof; a bottom mat interposed between the tray and the cartridge unit so as to block the bottom end of the cartridge unit; a fixing body combined with the top of the tray through a first coupling unit and pushes and fixes the outside of the cartridge unit; a top mat inserted on the fixing body so as to cover and seal the cartridge unit; a cover combined with the tray so as to cover and pressurize the top mat; and a purifying unit having a fixing groove formed therein in order to be inserted in and fixated to the top of the cartridge unit so that the chemical compound injected and synthesized in the cartridge unit can be purified.

Description

[0001] Apparatus for multiple reaction and purification of chemical [

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a chemical reaction and purification apparatus, and more particularly, to a chemical reaction apparatus and a chemical reaction apparatus which are capable of easily fixing a cartridge unit by covering a cartridge unit composed of a plurality of columns with an upper mat and a cover, To a chemical compound multiplex reaction and purification apparatus capable of injecting a compound of a cartridge portion into a purification cartridge at a time.

Generally, as the genome business grows, life sciences has started to study functional genomics, which converts information contained in the genome into protein information, understands their role, and regulates their function. The information obtained from functional genomics is expected to enable the treatment of most diseases faced by mankind by fully interpreting the functions and actions of living organisms.

The completion of the genome project will enable more than tens of thousands of new drug development projects to be developed for new drug development tasks that are simply hanging on hundreds of new disease development projects. Therefore, in order to link vast amounts of information obtained from the era of genome business to the development of practical new drugs, new drug candidate compounding technologies are inevitably required. It is necessary to solve the problem of developing tens of thousands of new medicines by participating in various scientific fields such as biology, mathematics, computer science, and chemistry and shortening the period of new drug development in the future. In developed countries, , And efforts to construct various compounds have been made in various fields such as the development of automatic compound synthesizer and the development of high-speed compound active search robot.

In the development of existing drugs, it takes a long time to develop a therapeutic agent even after the cause of the disease has been identified. In particular, a large number of compounds are required to derive a candidate substance that can be a therapeutic agent. In the course of the development of therapeutic agents in the last century, it is known that only one of about 10,000 compounds is put to practical use as a therapeutic agent. Now, as functional targets increase to tens of thousands of targets through functional genomics, more than hundreds of millions of compounds are needed right now to develop therapeutic agents. In this situation, it is important to synthesize new drug candidates quickly, efficiently, and in a large scale. The compounds thus constructed play a more important role in the study of finding disease-related targets by differentiating them from research methods for finding candidates in existing target identification.

As a result, recent trends in the development of new drugs have focused on technologies for simultaneously synthesizing tens of thousands of compounds in a so-called combinatorial chemistry to search for biological activity at a time. This is the most efficient technology in the process of finding new drug candidates or finding new drug candidates with high activity among leading drug derivatives.

Combinatorial chemistry has been developed since the 1960s on the basis of peptide synthesis and has been used to build many compounds. Until recently, compounds have been synthesized by combining compounds with solid supports. However, existing established compounds are constructed by focusing only on the number of compounds in a short period of time. As a result, all combinations chemically synthesized in the absence of purification of a substance that can act as a false positive in the actual compound activity search lost.

In the 1990s, the combined chemistry instruments developed a system of synthetic automation, but did not show the function of a well-refined compound due to small-scale traditional synthesis in introducing it into a compound activity search system. That is, when a compound is separated in the final stage of synthesis such as a polystyrene-based polymer compound used as a solid phase support when a compound is chemically synthesized, a substance not confirmed by UV spectrometer, NMR, IR, MS spectrometer, , There was a remarkable experimental difference in the retrieval activity between the compounds synthesized in the conventional manner and those purified purely in a silica gel column.

Due to these experimental errors, recent compounding has been shifted to the tendency to synthesize and build up compounds in the form of filtration, at least through purification. In other words, the fastest way to synthesize and refine a large number of compounds is to understand how to build the compounds needed for the development of new drugs.

Combination chemical reaction equipment and devices have already been well developed by numerous companies and are already being used by many chemists for the purpose of modifying equipment and devices on the market with the use of equipment, Has come. However, there is no application as a whole system except for the most characteristic one-by-one in most compound construction systems. In addition, the development including synthesis after purification is not carried out, and it has been necessary to purify the compound again or synthesized or existing mixture in order to search for the active activity of the compounds which have been synthesized as described above.

Therefore, it is now important to know how quickly a compound can be synthesized. However, how quickly a purely synthesized compound can simultaneously purify a large number of compounds in a short period of time is of great interest in compound construction.

Therefore, a new type of synthetic equipment should be developed to satisfy this requirement.

A related prior art is Korean Patent Laid-Open No. 10-2002-0023183.

In the present invention, a cartridge part composed of a plurality of columns is covered with an upper mat and a cover at the same pressure when the chemical compound is reacted, so that the cartridge part can be easily fixed. A fixing groove is formed on the upper part of the refining part, And to provide a chemical compound multi-reaction and purification apparatus that can be injected into a cartridge.

The technical objects to be achieved by the present invention are not limited to the above-mentioned technical problems.

In order to accomplish the above object, the chemical compound multi-reaction and purification apparatus of the present invention comprises: a tray in which a cartridge part composed of a plurality of columns storing a chemical compound is inserted, A lower mats interposed between the tray and the cartridge unit to block the lower end of the cartridge unit; a fixing body coupled to the tray via the first coupling unit to press and fix the outer side of the cartridge unit; And a cover which is inserted into the cartridge compartment and closes the cartridge compartment to seal the cartridge compartment, a cover that is coupled to the tray and covers the upper mat, presses the upper mat, And a tablet portion in which a fixing groove is formed in which the cartridge portion is inserted and fixed on the upper side Can.

Specifically, the tray may be formed of a clamp so that a first engaging portion is formed on an outer side of the supporting plate to fix the fixing body and the cover by pulling them.

The upper mat and the lower mat may be made of any material such as rubber or silicon, which is hermetic and has elasticity.

The refining unit is formed with a vacuum valve formed on an upper portion of a fixing groove through which the cartridge unit is inserted and fixed, and a cartridge for sucking a compound in the cartridge unit through a vacuum pump at one side to be injected into a purification cartridge having a plurality of fixing columns An upper frame coupled to a lower portion of the adapter through a second coupling portion to insert and fix the tablet cartridge on an upper side of the adapter, a lower frame coupled to a lower portion of the upper frame, And a lower mold in which a vacuum valve is formed in which a sieve is inserted and a compound in the purification cartridge is sucked into the storage body through a vacuum pump at one side.

The adapter, the upper frame and the lower frame may be coupled through a second coupling portion, and the second coupling portion may be formed in a clamped form.

As described above, according to the present invention, when the chemical compound is reacted, the cartridge part composed of a plurality of columns is covered and fixed with the upper mat and cover at the same pressure, so that the cartridge part can be easily fixed. There is an advantage that the negative composite can be injected into the refining cartridge at a time.

FIG. 1 is a plan view showing a configuration of a chemical compound multiplex reaction and purification apparatus according to an embodiment of the present invention.
FIG. 2 is a perspective view showing the configuration of a reaction unit for reacting a chemical compound according to the present invention in multiple reactions.
3 is a perspective view showing the structure of a purification unit for purifying a chemical compound according to the present invention in multiple stages.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same components are denoted by the same reference symbols whenever possible. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

FIG. 1 is a view showing an apparatus for multiple reaction and purification of a chemical compound according to an embodiment of the present invention, in which a cartridge part 30 composed of a plurality of columns for storing a chemical compound is inserted therein, A fixing mat 40 for fixing the cartridge unit 30 to the tray 10, a lower mat 20 to be inserted into the inner bottom of the tray 10, An upper mat 50 covering the upper side of the cartridge portion 30 inserted into the tray 10, a lid 60 covering and pressing on the upper mat 50, And a purification unit 2 for purifying a compound of the cartridge unit 30 that has undergone the reaction.

Generally, when a chemical compound is reacted and synthesized, one compound is combined with deprotection, coupling, capping and oxidation as one week.

This process proceeds in a column inserted and fixed in the reaction apparatus, and the synthesized compound in the reaction apparatus removes unnecessary substances through the purification apparatus.

Then, the purified compound is purified through a purification apparatus, and the preparation is completed.

The configuration of the reaction part 1 for reacting the chemical compound in this process is as follows.

1 and 2, the tray 10 includes an insertion groove 12 through which a lower mat 20 interposed between the cartridge unit 30 and the tray 10 is inserted and fixed, And a plurality of support rods are formed on the outer side and a first coupling portion 70 is formed on the outer side.

The tray 10 is made of a metal material and the lower mat 20 is inserted and fixed in the insertion groove 12 formed in the bottom plate of the tray 10 and the cartridge 10 30 are combined.

The lower mat (20) is made of any one of rubber or silicone which is hermetic and has elasticity.

A fixing unit 40 is coupled to the cartridge unit 30 to fix the cartridge unit 30 to the tray 10 to prevent the cartridge unit 30 from being detached or moved from the tray 10. [

The fixing member 40 is formed in a frame shape and presses the outer side of the cartridge unit 30 to be coupled through the first engaging portion 70 of the tray 10.

When the fixing body 40 is coupled to the upper side of the tray 10, the upper mat 50 covers and seals the upper side of the cartridge portion 30.

The upper mat (50) is made of rubber or silicone, which is a material having elasticity and hermeticity.

A lid 60 is covered on the upper mat 50 and a pressure is applied to the upper mat 50 by a pulling force of the first engaging portion 70 while being coupled through the first engaging portion 70 So that the cartridge portion 30 is sealed.

The reaction part 1 is constituted by the reaction part 1 and the chemical part is synthesized through the reaction part 1 and the reaction part 1 is separated from the reaction part 1, As shown in FIG.

When the cartridge part 30 is inserted into the fixing groove, the upper mat 50 covering the upper side is removed so that the compound of the cartridge part 30 can be injected into the refilling cartridge 90 of the refining part 2 do.

After the chemical synthesis is synthesized in the reaction part 1, the purification part 2 is purified in order to separate only the required compound.

As shown in Fig. 3, the configuration of the above-mentioned purification unit 2 is as follows.

The tablet part 2 is formed with a fixing groove in which the cartridge part 30 is fixed and a vacuum valve 140 connected to the outside by a vacuum pump and is formed into a frame shape. An upper frame 100 coupled to the lower side of the adapter 80 and fixed to the upper side of the tablet cartridge 90 so as to be fixed to the upper side of the upper frame 100, The adapter 80 and the upper frame 100 and the lower frame 110 are coupled through the second coupling unit 120 .

The adapter 80 has the cartridge part 30 inserted into the fixing groove on the upper side and the upper mat 50 is removed so that the vacuum valve 140 to which the vacuum pump is connected is opened, To be injected into the tablet cartridge 90.

The upper frame 100 is formed in a frame shape so that the tablet cartridge 90 is inserted and fixed on the upper side.

A lower frame 110 is coupled to the lower part of the upper frame 100 and a storage body 130 is inserted and fixed therein so that the purified compound in the purification cartridge 90 can be injected into the storage body 130 And a vacuum valve 140 to which a vacuum pump is connected is formed on the outside.

The vacuum valve 140 allows the purified compound in the purification cartridge 90 to be injected into the storage body 130 through a vacuum.

The adapter 80 is coupled to the upper frame 100 and the lower frame 110 through the second coupling portion 120. The second coupling portion 120 is formed in the same clamping configuration as the first coupling portion 70, .

The first engaging portion 70 and the second engaging portion 120 are provided with stoppers 72 and 122 formed on the fixing body 40, the lid 60 and the adapter 80, And assembled bodies (71, 121) formed on the frame (110).

The holding members 72 and 122 are provided with a U-shaped holding end at the lower end thereof and the coupling members 71 and 121 are fixed to the lower frame 110 and the tray 10, When the engaging member is pulled by the engaging end of the engaging members 72 and 122, the elastic body pulls the engaging member to pull the fixing member 40, the lid 60 and the adapter 80 from the tray The cover 60 and the adapter 80 are pulled by the elastic force of the elastic body.

Through the present invention, a chemical compound is reacted and synthesized, and the purification process is as follows.

The lower mat 20 is inserted and fixed in the insertion groove 12 formed on the upper side of the tray 10 and the cartridge part 30 is placed on the upper side of the lower mat 20, The cartridge 10 is pressed against the outer side of the cartridge part 30 to fix the cartridge part 30 and is coupled to the upper side of the tray 10 through the first engaging part 70. [

Then, a chemical compound material is injected into the column of the cartridge section 30, and the fixture 40 for holding the cartridge section 30 is pressed against the outer side of the cartridge section 30, So that the cartridge portion 30 is fixed.

The upper mat 50 is coupled to the upper side of the cartridge unit 30 to which the fixing unit 40 is coupled to seal the upper side of the cartridge unit 30. [

When the upper mat (50) covers and hermetically closes the cartridge part (30), the lid (60) is covered and joined through the first engaging part (70). At this time, since the lid 60 is pulled and joined by the first engaging portion 70, pressure is evenly applied to the entire lid 60, so that the whole cartridge can be uniformly sealed with the upper mat 50.

The reaction unit 1 thus configured is placed in the reaction chamber to perform reaction and is then separated from the cover 60 to be inserted into the fixing groove formed on the upper side of the adapter 80 of the tablet unit 2, (50) to the tablet part (2) at the same time.

When the cartridge part 30 is transferred to the tablet part 2 and inserted and fixed in the fixing groove, the upper mat 50 is removed and the compound stored in each column of the cartridge part 30 is removed from the inside of the column of the tablet cartridge 90 As shown in FIG.

The tablet cartridge 90 can be rapidly injected when the compound is injected into the tablet cartridge 90 through the suction force of the vacuum pump connected to the vacuum valve 140 formed outside the adapter 80, And is injected into the cartridge 90.

When the purification in the refill cartridge 90 is completed, the purified compound in the refill cartridge 90 is injected into the vacuum container 120 of the lower frame 110 to inject the purified compound into the storage container 130, 140 to operate the vacuum pump to rapidly store the compound in the purification cartridge 90 into the storage body 130 by the suction force.

When the composite material is stored in the storage body 130, the cartridge unit 30 and the adapter 80, the tablet cartridge 90 and the upper frame 100 are separated and removed, and the stored The body 130 is separated and put into the next process.

According to the present invention configured as described above, when the chemical compound is reacted, the cartridge part composed of a plurality of columns is covered and fixed with the upper mat and the cover at the same pressure, so that the cartridge part can be easily fixed, and a fixing groove is formed on the upper part of the purification part, Can be injected into the refining cartridge at a time.

Such a chemical compound multiply reaction and purification apparatus is not limited to the construction and operation of the embodiments described above. The embodiments may be configured so that all or some of the embodiments may be selectively combined so that various modifications may be made.

1: Reaction part 2: Purification part
10: tray 11: support plate
12: Insert groove
20: lower mat 30:
40: Fixture 50: Upper mat
60: Cover
70: first coupling portion 71: coupling
72:
80: Adapter 90: Tablet cartridge
100: upper frame 110: lower frame
120: second coupling portion 121: coupling body
122:
130: storage body 140: vacuum valve

Claims (5)

A tray in which a cartridge section composed of a plurality of columns in which a chemical compound is stored is inserted therein, and a plurality of support plates are formed in an outer upper direction;
A lower mat interposed between the tray and the cartridge unit to block the lower end of the cartridge unit;
A fixing body coupled to the tray via a first engaging portion and pressing the outer side of the cartridge portion;
An upper mat inserted in the fixing body to cover and seal the cartridge portion;
A cover coupled to the tray to cover the upper mat and press the upper mat; And
And a tablet part having a fixing groove inserted and fixed on an upper side of the cartridge part so as to purify the chemical compound injected into the cartridge part and synthesized,
Wherein the tablet part has an upper part formed with a fixing groove into which the cartridge part is inserted and fixed and a vacuum valve is formed to suck a compound in the cartridge part through a vacuum pump at one side thereof and to inject the purified compound into a tablet cartridge, ;
An upper frame coupled to a lower portion of the adapter through a second engaging portion and having the tablet cartridge inserted and fixed on the upper side; And
A vacuum valve coupled to a lower portion of the upper frame for inserting therein a storage body for storing a compound injected from the tablet cartridge and sucking a compound in the tablet cartridge through a vacuum pump at one side to be injected into the storage body; And a formed lower frame.
The method according to claim 1,
Wherein said tray comprises a clamp formed by a first engaging portion formed on the outside of said support plate to pull and fix said fixture and said lid.
The method according to claim 1,
Wherein the upper mat and the lower mat are made of one material selected from the group consisting of rubber or silicon, which has a hermetic property and has elasticity.
delete The method according to claim 1,
Wherein the adapter, the upper frame, and the lower frame are coupled through a second coupling portion, and the second coupling portion is formed in a clamped form.

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