KR101372234B1 - Optical Sensor using the nano spacer and Detecting Method using the Optical Sensor - Google Patents

Abstract

An optical sensor using a nano spacer and a detection method using an optical sensor are provided. An optical sensor using a nano spacer according to an embodiment of the present invention includes a nano spacer having a size reversibly changed with temperature, a first object coupled to one side of the nano spacer, a second object coupled to the other side of the nano spacer, and It may be configured to include a detector for detecting the light emitted by the interaction between the first object and the second object. Thus, the optical properties between nanoparticles, ie fluorescence, fluorescence resonance energy transfer (FRET), localized surface plasmon resonance (LSPR), and surface, using nano spacers whose length varies reversibly with temperature Optical phenomena such as enhanced Raman spectroscopy (SERS) can be actively controlled. In addition, the detection limits of optical sensors based on the sensing principle using fluorescence, fluorescence resonance energy transfer (FRET), localized surface plasmon resonance (LSPR), and surface enhanced Raman spectroscopy (SERS) using the nano spacer. Can improve.

Description

Optical Sensor using the nano spacer and Detecting Method using the Optical Sensor}

The present invention relates to an optical sensor using a nano spacer and a detection method using an optical sensor, and more particularly, an optical sensor using a nano spacer and a detection method using an optical sensor that can control and detect optical characteristics by an external stimulus. It is about.

The present invention is derived from a study performed as part of the IT source technology development project of the Ministry of Knowledge Economy [Task management number: 2006-S-007-02, Task name: Ubiquitous health management module system].

Fluorescence refers to a phenomenon in which a substance emits light by stimulation of light. A material that receives light energy produces light with a wavelength longer than the wavelength of the incident light (ie, lower energy than the original), which is different from reflection. Fluorescence is distinguished by the fact that it continues to emit light even if the light is removed.

Fluorescence Resonance Energy Transfer (FRET) utilizes the characteristics of a pair of energy receptors, energy donors, and a pair of biomolecules with specific binding.

When an energy donor, a shorter wavelength dye, absorbs energy from the outside, the excitation energy of the donor is emitted as light energy, instead of being emitted by an energy acceptor, a longer wavelength excitation dye. Radiationless delivery refers to a phenomenon in which only the long wavelength fluorescence of the receptor is emitted.

Surface Plasmon refers to surface electromagnetic waves propagating along the interface between metal and dielectric. The surface plasmon can be strengthened according to the change of the surface or structure of the metal, which can be applied to biomolecule analysis technology and optical device development.

Localized Surface Plasmon Resonance (LSPR), an example of surface plasmon, selectively absorbs the energy of incident light depending on the size and shape of the metal nanoparticles and the dispersion properties of the surrounding medium. Scattering phenomenon. This means that light energy is absorbed and converted by the surface plasmons, and the electric field formed on the surface of the metal nanoparticles is locally distorted and strengthened, which means that light can be controlled in a region smaller than the diffraction limit of the light, that is, nearfield. do.

Raman Spectroscopy is a well-known technique that provides information about the vibrational frequencies of molecules. In addition, Surface Enhanced Raman Scattering (SERS) is a phenomenon in which the Raman signal is greatly increased when there are molecules near the metal surface.

Raman Spectroscopy (Rman Spectroscopy) is difficult to apply to real life because the signal strength is weak compared to fluorescence, but the surface enhanced Raman Scatter (SERS) phenomenon can overcome this difficulty.

In general, Surface Enhanced Raman Scattering (SERS) phenomenon is known to occur when molecules are nearby by surface plasmon resonance (Surface Plasmon Resonance) of silver or gold nanoparticles.

In general, fluorescence and quenching of fluorescent particles, fluorescence resonance energy transfer (FRET), localized surface plasmon resonance (LSPR) and metal-enhanced Raman spectroscopy of metal nanoparticles Surface Enhanced Raman Spectroscopy (SERS) phenomena have something in common that is very sensitive to the distance between nanoparticles or the metal / dielectric surface in close proximity to the particles.

However, since the medium for determining the distance between the nanoparticles is generally fixed in length, there is a disadvantage in that the energy transfer conditions between the nanoparticles cannot be actively controlled.

That is, there is a need for an optical sensor that can actively adjust the energy transfer conditions between nanoparticles. In particular, to address the shortcomings of conventional spacers with a fixed length, there is a need for optical sensors using nano spacers whose lengths can be reversibly changed for external stimuli such as temperature, pH, light intensity and wavelength of light. There is this.

An object of the present invention is to provide an optical sensor using a nano spacer that can control and detect the optical characteristics by an external stimulus.

Another object of the present invention is to provide a detection method using an optical sensor that can control and detect optical characteristics by an external stimulus.

Optical sensor using a nano-spacer for achieving the above object of the present invention comprises a nano-spacer that reversibly changes in size with temperature; A first object coupled to one side of the nano spacer; A second object coupled to the other side of the nano spacer; And a detector for detecting light emitted by the interaction between the first object and the second object.

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Here, the temperature may be one having a critical condition in which the size of the nano spacers is changed.

Here, the length between the first object and the second object may be changed according to the size change of the nano spacer.

Here, the nano spacer may be formed including at least one of a polymer and a hydrogel.

Here, the nano spacer may be PNIPAAm (poly N isopropylacrylamide).

Here, the interaction between the first object and the second object may be expressed by any one of fluorescence and fluorescence resonance energy transfer (FRET), and the first object and the second object may be phosphors.

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Here, the interaction between the first object and the second object is represented by any one of local surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS), the first object is a phosphor, the second object is It may be a metal nanoparticle.

Here, the detector may detect at least one of a change in intensity of light and a change in wavelength of light emitted by the interaction between the first object and the second object according to the size change of the nano spacer.

In the detection method using the optical sensor to achieve the above object of the present invention, the first object is coupled to one side of the nano spacer whose size reversibly changes with temperature, and the second object is attached to the other side of the nano spacer. Combining; Applying the temperature to the bonded nano spacers; And detecting light emitted by the interaction between the first object and the second object.

Here, the nano spacer may be formed including at least one of a polymer and a hydrogel.

Here, the nano spacer may be PNIPAAm (poly N isopropylacrylamide).

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Here, the temperature may be one having a critical condition in which the size of the nano spacers is changed.

Here, the temperature may be changed periodically in the step of applying the temperature.

The detecting of the emitted light may include detecting at least one of a change in intensity of the emitted light and a change in wavelength of light according to the change in temperature.

Here, the interaction between the first object and the second object may be expressed by any one of fluorescence and fluorescence resonance energy transfer (FRET), and the first object and the second object may be phosphors.

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Here, the interaction between the first object and the second object is represented by any one of local surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS), the first object is a phosphor, the second object is It may be a metal nanoparticle.

According to the optical sensor using the nano-spacer and the detection method using the optical sensor as described above, the optical characteristics between the nanoparticles, that is, fluorescence using nano spacers whose lengths are reversibly changed according to external stimuli. Optical phenomena such as fluorescence resonance energy transfer (FRET), localized surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS).

In addition, detection limits of optical sensors based on the sensing principle of fluorescence, fluorescence resonance energy transfer (FRET), localized surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS) using the nano spacers Can improve.

1 is an exemplary view for explaining an optical sensor using a nano spacer according to an embodiment of the present invention.
2 is an exemplary diagram for explaining fluorescence resonance energy transfer (FRET) in an optical sensor using a nano spacer according to an embodiment of the present invention.
3 is an exemplary view for explaining local surface plasmon resonance (LSPR) in an optical sensor using a nano spacer according to an embodiment of the present invention.
4 is a flowchart illustrating a detection method using an optical sensor according to an embodiment of the present invention.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail.

It should be understood, however, that the invention is not intended to be limited to the particular embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component. And / or < / RTI > includes any combination of a plurality of related listed items or any of a plurality of related listed items.

When a component is referred to as being "connected" or "connected" to another component, it may be directly connected to or connected to that other component, but it may be understood that other components may be present in between. Should be. On the other hand, when an element is referred to as being "directly connected" or "directly connected" to another element, it should be understood that there are no other elements in between.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this application, the terms "comprise" or "having" are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, and one or more other features. It is to be understood that the present invention does not exclude the possibility of the presence or the addition of numbers, steps, operations, components, components, or a combination thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the relevant art and are to be interpreted in an ideal or overly formal sense unless explicitly defined in the present application Do not.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In order to facilitate the understanding of the present invention, the same reference numerals are used for the same constituent elements in the drawings and redundant explanations for the same constituent elements are omitted.

1 is an exemplary view for explaining an optical sensor using a nano spacer according to an embodiment of the present invention.

Referring to FIG. 1, an optical sensor 100 using a nano spacer according to an embodiment of the present invention may include a nano spacer 110 having a size reversibly changed according to an external stimulus, and a first coupled to one side of the nano spacer. An object 120, a second object 130 coupled to the other side of the nano spacer, and a detector 140 for detecting light emitted by the interaction between the first object and the second object. Can be.

First, the external stimulus may be at least one of temperature, humidity, pH, light intensity, and light wavelength, and external stimuli such as temperature, humidity, pH, light intensity, and light wavelength may be respectively applied to the nano spacers. It may have a threshold condition that changes in size.

Next, the nano spacer 110 may be formed to include at least one of a polymer and a hydrogel, and in particular, may be composed of PNIPAAm (poly N isopropylacrylamide).

Next, the first object 120 and the second object 130 may be phosphors and metal nanoparticles, respectively. Accordingly, the interaction between the first object and the second object is represented by any one of fluorescence, fluorescence resonance energy transfer (FRET), local surface plasmon resonance (LSPR), and surface enhanced Raman spectroscopy (SERS). Can be.

In detail, when the interaction between the first object and the second object is represented by any one of fluorescence and fluorescence resonance energy transfer (FRET), the first object and the second object may be phosphors. When the interaction between the first object and the second object is expressed by one of local surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS), the first object is a phosphor, and the second object is a metal nanoparticle. Can be.

Next, the detection unit 140 may detect at least one of a change in intensity of light and a change in wavelength of light emitted by the interaction between the first object and the second object according to the size change of the nano spacer. have.

As a result, the external stimulus is applied to the combination of the nano spacer 110, the first object 120, and the second object 130, where the external stimulus may change the size of the nano spacer. It may be there.

According to the size change of the nano spacer 110, the distance between the first object 120 and the second object 130 may change, and the first object 120 and the second object 130 may change. According to the distance change of the liver, it is possible to detect the characteristic of changing the light intensity by fluorescence, fluorescence resonance energy transfer (FRET), local surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS). .

2 is an exemplary diagram for explaining fluorescence resonance energy transfer (FRET) in an optical sensor using a nano spacer according to an embodiment of the present invention.

Referring to FIG. 2, in the optical sensor 100 using the nano spacer according to an embodiment of the present invention, the interaction between the first object 120 and the second object 130 is fluorescence resonance energy transfer (FRET). Look for the phenomenon. Here, the first object 120 may be a phosphor, and the second object 130 may also be a phosphor.

Before the external stimulus is applied, a distance between the first object 120 and the second object 130 by the nano spacer can be maintained as shown in FIG. 2 (a). Accordingly, the first object 120 can be maintained. There is no fluorescence resonance energy transfer (FRET) to the second object 130 from), it can be detected that the scattering of the first object 120 or the intensity of the fluorescent light is not reduced.

However, after the external stimulus is applied, the distance between the first object 120 and the second object 130 by the nano spacer is reduced to smaller as shown in FIG. 2 (b), and accordingly the first object ( As an interaction between the 120 and the second object 130, a quenching phenomenon in which fluorescence resonance energy transfer (FRET) occurs from the first object 120 to the second object 130 occurs. That is, it will be detected that the intensity of light emitted by the fluorescence resonance energy transfer (FRET) phenomenon is reduced.

According to the change of the external stimulus, at least one of the intensity change and the wavelength change of the light emitted by the fluorescence resonance energy transfer (FRET) phenomenon can be detected, the first object 120 and the first It is possible to detect the characteristics of the interaction between the two objects (130).

3 is an exemplary view for explaining local surface plasmon resonance (LSPR) in an optical sensor using a nano spacer according to an embodiment of the present invention.

Referring to FIG. 3, in the optical sensor 100 using the nano spacer according to an embodiment of the present invention, the interaction between the first object 120 and the second object 130 is local surface plasmon resonance (LSPR). Look for the phenomenon. Here, the first object 120 may be a phosphor, and the second object 130 may be a metal nanoparticle.

Before the external stimulus is applied, a distance between the first object 120 and the second object 130 by the nano spacer can be maintained as shown in FIG. 3 (a), and accordingly, the first object 120 can be maintained. There is no non-radiative energy transfer (non-radiative energy transfer) to the second object 130 from), it can be detected that the scattering or the intensity of the fluorescent light of the first object 120 is not reduced.

However, after the external stimulus is applied, the distance between the first object 120 and the second object 130 by the nano spacer is reduced as shown in FIG. 3 (b), and accordingly, the first object ( The non-radiative energy transfer occurs from the first object 120 to the second object 130 by the interaction between the first object 120 and the second object 130. When the energy (ie, wavelength) corresponds to the local surface plasmon resonance (LSPR) energy (ie, resonance wavelength) of the second object, ie, the metal nanoparticle, the intensity of light emitted from the second object 130 is large. Will be detected.

According to the change of the external stimulus, at least one of a change in intensity of light and a change in wavelength of light emitted by a local surface plasmon resonance (LSPR) phenomenon can be detected. It is possible to detect the characteristics of the interaction between the two objects (130).

4 is a flowchart illustrating a detection method using an optical sensor according to an embodiment of the present invention.

Referring to FIG. 4, in a detection method using an optical sensor according to an exemplary embodiment, a first object is coupled to one side of a nano spacer whose size is reversibly changed according to an external stimulus, and the other side of the nano spacer is connected to the first object. Coupling a second object (step 410), applying the external stimulus to the bonded nano spacer (step 420), and detecting light emitted by the interaction between the first object and the second object. It may be configured to include a step (step 430).

First, the nano spacer may be formed including at least one of a polymer and a hydrogel, and the nano spacer may be PNIPAAm (poly N isopropylacrylamide).

Next, the step of coupling the first object to one side of the nano spacer reversibly changes in size according to the external stimulus, and the second object to the other side of the nano spacer (step 410) is the optical using the nano spacer Since the sensor has been described above, a detailed description thereof will be omitted.

Next, the step of applying the external stimulus to the bonded nano spacer (step 420) is a temperature, humidity, pH, light intensity and wavelength of light to the combination of the nano spacer, the first object and the second object At least one of the external stimulus may be applied.

Herein, the external stimulus may be one having a threshold condition in which the size of the nano spacers is changed, and in particular, the external stimulus may be periodically changed at step 420 of applying the external stimulus. Accordingly, the characteristics according to the change of the external stimulus will be examined.

Next, detecting the light emitted by the interaction between the first object and the second object (step 430) may include changing the intensity of light emitted by the interaction between the first object and the second object and It may be to detect at least one of the wavelength change of the light.

In particular, in the step of detecting the emitted light (step 430), the interaction between the first object and the second object is fluorescence, fluorescence resonance energy transfer (FRET), local surface plasmon resonance (LSPR) and surface It may be represented by any one of enhanced Raman spectroscopy (SERS).

Accordingly, when the interaction between the first object and the second object is represented by any one of fluorescence and fluorescence resonance energy transfer (FRET) so as to be suitable for detection, the fluorescent material is defined as the first object and the second object. When the interaction between the first object and the second object is represented by one of local surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS), the first object may include a phosphor and the second object. Metal nanoparticles may be used as the object.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined in the appended claims. It will be possible.

100: optical sensor using nano spacer
110: nano spacer 120: first object
130: second object 140: detector

Claims (20)

A nano spacer whose size reversibly changes with temperature;
A first object coupled to one side of the nano spacer;
A second object coupled to the other side of the nano spacer; And
It includes a detection unit for detecting the light emitted by the interaction between the first object and the second object,
The interaction between the first object and the second object is expressed by any one of fluorescence and fluorescence resonance energy transfer (FRET), wherein the first object and the second object is an optical sensor, characterized in that the phosphor. delete The method of claim 1,
And said temperature has a threshold condition in which the size of the nano spacers changes. The method of claim 1,
The optical sensor according to claim 1, wherein the length between the first object and the second object changes according to the size change of the nano spacer. The method of claim 1,
The nano spacer is formed by including at least one of a polymer (polymer) and a hydrogel (hydrogel). 6. The method of claim 5,
The nano spacer is an optical sensor, characterized in that the poly (N-isopropylacrylamide). delete delete A nano spacer whose size reversibly changes with temperature;
A first object coupled to one side of the nano spacer;
A second object coupled to the other side of the nano spacer; And
It includes a detection unit for detecting the light emitted by the interaction between the first object and the second object,
The interaction between the first object and the second object is expressed by any one of local surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS), the first object is a phosphor, the second object is a metal nano Optical sensor, characterized in that the particles. 10. The method of claim 1 or 9,
The detector detects at least one of a change in intensity of light and a change in wavelength of light emitted by the interaction between the first object and the second object according to the size change of the nano spacer. Coupling a first object to one side of the nano spacer, the size of which reversibly changes with temperature, and a second object to the other side of the nano spacer;
Applying the temperature to the bonded nano spacers; And
Detecting light emitted by the interaction between the first object and the second object,
The interaction between the first object and the second object is expressed by any one of fluorescence and fluorescence resonance energy transfer (FRET), wherein the first object and the second object is an optical sensor, characterized in that the phosphor Detection method used. 12. The method of claim 11,
The nano spacer is a detection method using an optical sensor, characterized in that it comprises at least one of a polymer (polymer) and a hydrogel (hydrogel). 12. The method of claim 11,
The nano spacer is a detection method using an optical sensor, characterized in that poly (N-isopropylacrylamide). delete 12. The method of claim 11,
The temperature is a detection method using an optical sensor, characterized in that the threshold condition that the size of the nano-spacer changes. 12. The method of claim 11,
In the step of applying the temperature, the temperature is a detection method using an optical sensor, characterized in that for changing periodically. 12. The method of claim 11,
The detecting of the emitted light may include detecting at least one of a change in intensity of the emitted light and a change in wavelength of light according to the change in temperature. delete delete Coupling a first object to one side of the nano spacer, the size of which reversibly changes with temperature, and a second object to the other side of the nano spacer;
Applying the temperature to the bonded nano spacers; And
Detecting light emitted by the interaction between the first object and the second object,
The interaction between the first object and the second object is expressed by any one of local surface plasmon resonance (LSPR) and surface enhanced Raman spectroscopy (SERS), the first object is a phosphor, the second object is a metal nano Detection method using an optical sensor, characterized in that the particles.

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