KR101345336B1 - Composition for enhancing blood circulation containing modified Jeho-Tang extract as an active ingredient - Google Patents

Abstract

The present invention relates to a blood circulation improving composition containing an extract of Gagamjeho-tang as an active ingredient, and more particularly, to a blood circulation improving composition containing a mixed extract of five plum meat, sine, excess and sandalwood as an active ingredient, a preparation method thereof, and a use thereof. to provide.

The mixed extract according to the present invention not only has an excellent inhibitory effect on whole blood coagulation ability, platelet membrane receptor expression, platelet calcium mobilization, etc., but also has an excellent effect on inhibiting blood flow adhesion induced by hemocytosis stress, such as atherosclerosis, cerebral hemorrhage, and stroke. , Cerebral infarction and the like can be usefully used for the prevention and treatment of diseases associated with blood circulation disorders.

Falcon meat, excess, sine, sandalwood, blood circulation, thrombosis

Description

Composition for enhancing blood circulation containing modified Jeho-Tang extract as an active ingredient

1 is a view showing the whole blood coagulation inhibitory effect of the mixed extract according to an embodiment of the present invention.

Figure 2 is a diagram showing the blood cell adhesion inhibitory effect of the mixed extract according to an embodiment of the present invention.

The present invention relates to a blood circulation improving composition containing gagamjehotang extract as an active ingredient.

Recently, circulatory diseases such as arteriosclerosis, cerebral hemorrhage, hypertension, heart disease, stroke and cerebral infarction are the leading causes of death in Korea as well as developed countries. As adult diseases develop into modern society, they are frequently occurring in middle-aged and middle-aged people due to changes in dietary habits and internal and external environmental stresses. Vascular blockage may occur due to various causes, such as cardiovascular blockage, myocardial infarction may occur, and when blood vessels in the brain are blocked, cerebral infarction may occur. In the case of cerebral infarction, the disease may continue to progress, resulting in a stroke and death. In the United States, stroke is the third most common cause of death in adults and is known to be the most important cause of mobility. The main cause of these diseases is known as thrombus, which is recognized as a pathology mediated by excessive platelet aggregation. In addition, this also causes a disorder of blood circulation.

Hemostasis is a mechanism that prevents bleeding from blood vessels whose original shape has been destroyed, through the complex process of platelet activation, adhesion of platelets and vascular components, and the associated blood coagulation factors to restore damaged blood vessels (Harlan and Harker, Med. Clin . North . Am ., 65: 855, 1981). On the other hand, platelets are activated by stimulation of various agglomerates such as collagen, arachidonic acid, and aDP (adenosine diphosphate) during blood vessel damage, thereby adhering to adhesion and secretion. And aggregate aggregation. These reactions are associated with haemostasis and are known to be involved in the development of circulatory diseases, including thrombosis and the like.

Platelets have a characteristic discoid form at rest, but when activated, they lose their inherent shape and undergo an irregular shape with fictitious shapes, and polymerize cytoskeletons. ), The result of phosphorylation and thus interaction (Nachmias et al., Nature , 313: 70, 1985).

Antiplatelet agents developed to date include theopylline, molsidomin, verapamil and nifedipine. It is known for promoting the formation of cAMP and cGMP inhibit the mobilization of Ca ^{+ 2.} Nonsteroidal compounds such as aspirin, imidazole, and indomethacin are also known as drugs having antiplatelet action by inhibiting the production of thromboxane A ₂ . However, drugs made of the chemicals have a problem of causing various side effects such as excessive hemostasis suppression, infertility, digestive disorders in the human body. Accordingly, in order to find a substance having the same effect while reducing side effects of the drug made of the chemical substance, a lot of researches on substances that improve blood circulation from natural products or herbal prescriptions have been conducted.

Jeho-tang was used as a medicine to quench the heat and stop thirst in the palace. Jeho-tang is a traditional Korean soft drink that is said to relieve the heat caused by eating heat from Dongbobogam and medicinal herbs, and to replace it. The Jeho-tang is composed of five roots of five plums, one cup of excess, sine and sandalwood, and 5 knots of honey and 5 knots of honey, and it is recorded that the medicines are finely powdered and boiled with honey to stir evenly in cold water.

Although there are many records on Jeho-tang, few studies have been conducted on the efficacy of Jeho-tang except for studies on intestinal bacteria and immune activity (Ji, Myung-Soon et al., Korean Journal of Food Science and Technology, 2006). No studies have been reported.

Conventionally, the Republic of Korea Patent No. 417244 has the effect of inhibiting the activity of elastase (elastase), thereby maintaining the elasticity of the blood vessels, NO inhibitory activity involved in blood coagulation and healing of wounds in the blood vessel walls, blood circulation It has the effect of stimulating u-PA and inhibiting PAI-1, and it is effective in dissolving blood clots to improve blood circulation. Therefore, it will be effective in treating and preventing cardiovascular diseases caused by disorders in blood circulation. It relates to a pharmaceutical composition for improving blood circulation containing a powder extract can be described.

In addition, the Republic of Korea Patent No. 345040 describes a blood circulation improving agent and a method for manufacturing the red ginseng complex containing red ginseng complex after extracting kelp, ganoderma lucidum, cinnamon, licorice and the like in red ginseng.

However, Patent Nos. 417244 and 345040 do not describe compositions for improving blood circulation, including maeyuk, excess, sine, sandalwood, etc. to improve blood circulation.

Accordingly, the present inventors found that the composition consisting of Gagamjeho-tang was effective in inhibiting whole blood cohesion, blood cell adhesion stress induced by blood cell strain stress, and the like while studying blood circulation improving agents using herbal medicines or herbal medicines. The study was repeated to complete the present invention.

An object of the present invention to provide a composition for the prevention and treatment of thrombotic diseases by improving blood circulation.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the prevention and treatment of thrombosis disease by improving blood circulation, containing a mixed extract of maeyang meat, sine, excess and sandalwood as an active ingredient.

The present invention also provides a food composition comprising a mixed extract of five buckwheat, sine, excess and sandalwood and a food supplement acceptable food supplement additive.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for the prevention and treatment of thrombotic diseases by improving blood circulation, which contains a mixed extract of maeyang meat, sine, excess and sandalwood as an active ingredient.

Ome (는 梅) is an immature fruit of plum tree, fumigation with straw fire, dry, warm, non-poisonous and delicious. Fence, vomiting, thirst and dysentery can be used and alcohol can be used. It is mainly used for capping and jogging. Eliminate black warts and cure dry spitting often (consent, herbal).

Excess (草 果) is warm in nature, tastes very good and nontoxic. It is mainly used for all kinds of chills. Warm the stomach and stop vomiting. Healing the abdominal swelling, eliminating school heads, eliminating bed and breakfast, releasing liquor and fruit decay, defeating the long-term erosion caused by moxibustion and freeing the whole area (agreement, initiation).

Sine (砂仁) is warm in nature, taste is very poisonous. Evens the inside, lowers the diarrhea and diarrhea. Provoke appetite and digestion well (agreement, herbal).

Sandalwood (白檀香) is warm in nature, taste is very poisonous. Relieve the swollen fever and treat the pain in the stomach due to wear (agreement, herbal).

The content of the mixed composition of the present invention is preferably 18 to 22 parts by weight, 1 to 4 parts by weight, 1 to 3 parts by weight, and 1 to 3 parts by weight of sandalwood.

The method for preparing the mixed extract of maeyong meat, sine, excess and sandalwood may be extracted by conventional methods in the art, and preferably the maeyuk, sine, excess and sandalwood is water, methanol, ethanol or a mixture thereof. It can be immersed in a polar solvent such as a solvent and heated to prepare a mixed extract. More preferably, after grinding the maeyeok meat, sine, excess and sandalwood flavor, 18 to 22 parts by weight, sine 1 to 4 parts by weight, excess 1 to 3 parts by weight and 1 to 3 parts by weight of sandalwood, and mix the mixture 1 g After 10 ml of sugar is added, it can be extracted by heating. Extraction method is preferably extracted under reflux heating for 2 hours at 70 ~ 90 ℃. In order to increase the extraction efficiency, the above extraction may be repeated several times or more. After cooling the extract obtained through the extraction method, it was filtered again, concentrated on an aqueous solution of 35 ~ 40 ℃ to obtain an extract. The concentration method may be concentrated by a conventional method in the art, preferably concentrated under reduced pressure using a rotary vacuum concentrator. The concentrated extract may be dried to prepare a powder, and the method for preparing the powder is not particularly limited, but after freezing by adding water to the concentrated extract, it is dried using a lyophilizer to prepare a powder. can do.

When the whole blood coagulation inhibitory activity of the mixed extract according to the present invention and Jeho-tang prepared as far away was measured, the Jeho-tang had a high concentration of whole blood aggregation (5000 μg / ml), while the mixed extract according to the present invention was low. Show whole blood aggregation concentration.

In addition, the platelet membrane receptor expression inhibitory effect of the mixed extract according to the present invention, when ADP was administered as a platelet activity inducer, it was found that the expression of receptors such as PAC-1, p-selectin, platelet membrane receptor expression factor was increased, When treating the mixed extract according to the present invention, platelet membrane receptor expression is reduced.

In addition, the inhibitory effect on the platelet calcium mobilization of the mixed extract according to the present invention is lower than the platelet calcium mobilization inhibitory effect (MFI) value shown in the maximum response of the control group treated with phosphate buffered water only. Indicates.

In addition, the adhesion ability of the blood cells induced by hemocytosis stress to the collagen coating slides was not significantly different from the control group treated with phosphate buffered saline in the collagen coating slides in the case of Jeho-tang-treated samples. On the other hand, it can be seen that the blood cell adhesion is greatly reduced in the case of the sample treated with the mixed extract according to the present invention.

In view of the above results, the mixed extract according to the present invention not only has an excellent inhibitory effect on whole blood aggregation ability, platelet receptor expression, platelet calcium mobilization, etc., but also has an effect on inhibiting blood flow adhesion induced by hemocytosis stress. . Therefore, the mixed extract according to the present invention can be usefully used as a pharmaceutical composition for the prevention and treatment of thrombotic diseases by improving blood circulation.

The thrombosis is not particularly limited as long as it is a disease that can be caused by problems in blood clot production and blood circulation, and the mixed extract according to the present invention is particularly useful for diseases such as atherosclerosis, cerebral hemorrhage, stroke, and cerebral infarction. Can be used.

The compositions according to the invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers for the prevention and treatment of diseases involving thrombus formation.

The composition according to the present invention may be prepared by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration. Pharmaceutically acceptable carriers may be used in combination with saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components, if necessary, as antioxidants, buffers And other conventional additives such as bacteriostatic agents can be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Further, it can be suitably formulated according to each disease or ingredient, using appropriate methods in the art or by the method disclosed in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA.

The composition according to the invention can be administered orally or parenterally (eg, applied intravenously, subcutaneously, intraperitoneally or topically) according to the desired method, and the dosage is based on the weight, age, sex, health of the patient. The range varies depending on the condition, diet, time of administration, method of administration, rate of excretion and the severity of the disease. The daily dose of the mixed extract according to the present invention is 46 ± 50 mg / kg, preferably 46 ± 10 mg / kg, preferably administered once to three times a day.

The present invention also provides a food composition comprising a mixed extract of five buckwheat, sine, excess and sandalwood and a food supplement acceptable food supplement additive.

The mixed extract may contain 18-22 parts by weight of maeyuk, 1-4 parts by weight, excess 1-3 parts by weight and 1-3 parts by weight of sandalwood. The preparation of the mixed extract may be used by preparing the mixed extract by immersing the maeyuk meat, sine, excess and sandalwood in a polar solvent such as water, methanol, ethanol or a mixed solvent thereof.

The composition according to the present invention may be added to a health food for the purpose of improving diseases caused by blood circulation disorders, and when the mixed extract of the present invention is used as a food additive, the herbal medicines may be added as it is or mixed with other foods or food ingredients. It can be used together with, and can be suitably used according to a conventional method. The blending amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). Generally, the mixed herbal medicine of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material in the manufacture of food or beverage. However, in the case of long-term intake for health and hygiene purposes or health control purposes, the amount may be below the above range, and since there is no problem in terms of stability, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, dairy products including other noodles, gums, and ice cream, various soups, beverages, teas, and drinks. Alcoholic beverages and vitamin complexes, and includes all of the health foods in the conventional sense.

The food supplement additive of the present invention may use various flavors or natural carbohydrates. The above-mentioned natural carbohydrates are glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as tau martin, stevia extract, synthetic sweeteners such as saccharin, aspartame, and the like can be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 parts by weight, preferably 0.02 to 0.03 parts by weight per 100 parts by weight of the composition according to the present invention.

In addition to the above, the composition according to the present invention contains various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols. And carbonation agents used in carbonated beverages. In addition, the composition according to the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components can be used independently or in combination, and the proportion of the additive is not critical, but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition according to the present invention.

Hereinafter, the present invention will be described in more detail by the following examples and experimental examples. However, the following Examples and Experimental Examples are only illustrative of the contents of the present invention and the scope of the invention is not limited by the Examples and Experimental Examples.

< Example  1> hawk, sine, excess and Sandalwood  Preparation of mixed extracts

Purchasing ume, sine, excess, and sandalwood were checked by the Korean Institute of Oriental Medicine for external morphology, and then the medicinal herbs were quantified as 40 g, sine, 4 g, excess 2 g, and sandalwood 2 g. It was prepared by mixing the powder. 40 g of the mixed sample was added to 400 ml of distilled water and refluxed for 2 hours. The mixture was left at room temperature and filtered through a filter paper. The filtrate was centrifuged at 10,000 rpm for 15 minutes to give a supernatant, and freeze-dried at -70 ° C to prepare a mixed extract (9.91 g, yield 24.8%). It was stored in a cool dark place.

Comparative Example 1 Preparation of Jeho-tang Extract

20 g, 5 g of sine, 1 g of excess, 1 g of sandalwood, and 150 g of honey were quantified and then mixed into powder using a mixer. 50 g of the mixed sample was added to 500 ml of distilled water, followed by extraction under reflux for 2 hours, and the mixture was left at room temperature and filtered through a filter paper. The filtrate was centrifuged at 10,000 rpm for 15 minutes, the supernatant was taken, and lyophilized at -70 ° C to prepare a mixed extract of Jeho-tang (34.32 g, yield 68.64%). It was stored in a cool dark place.

ingredient

Content (weight ratio)
Example 1
Comparative Example 1 Oh 20 20 Excess 2 2 sign One One Sandalwood One One honey 150

< Experimental Example  1> whole blood Cohesion  Inhibitory effect measurement ( In vitro )

In order to determine the inhibitory effect of whole blood coagulation of mae, sine, excess and sandalwood mixed extract, the following experiment was performed.

Eight adult women in their 20s and 30s were randomly selected and were not given aspirin or nonsteroidal anti-inflammatory drugs a week before the blood was drawn. At a given time, blood was drawn with a 5 ml disposable plastic syringe and then dispensed into a vacuum tube (vaccutainer, Becton Dickinson, USA) containing 3.2% sodium citrate anticoagulant to prevent coagulation. Then, the whole blood coagulation inhibitory activity was measured using a whole blood clotting analyzer (aggregometer, Chrono Log, USA). Dilute with physiological saline so that platelet count of whole blood is 4 × 10 ⁸ cells / ml, and incubate for 10 minutes at 37 ° C., and 50 μl of the mixed extract of Example 1 and Comparative Example 1 (concentration dependent at the highest concentration of 5 mg / ml). Step dilution) was added for 5 minutes. The platelet aggregation inducer (collagen 2 ㎍ / ㎖, Chrono Log, USA) was put in a certain amount and reacted at 37 ℃ while stirring at 1,000 rpm to determine the degree of whole blood aggregation as a change in impedance (impedance). The results are shown in Table 1 and Fig.

Statistical processing of the experimental results was carried out using the SPSS package, all measurements were expressed as mean ± standard deviation. In order to verify the significance of the analysis, independent sample T-TEST was conducted to analyze the significance of the mean of the analysis results.

Inhibitory Effect on Whole Blood Coagulation of Mixed Extracts According to the Present Invention
sample
IC ₅₀ concentration (μg / ml) Example 1 526.3 Comparative Example 1 > 5000

 In Figure 1 (a) to analyze the impedance of whole blood aggregation to determine the inhibitory concentration, using this to obtain the inhibitory concentration curve of Figure 1 (b). In addition, 50% inhibition concentration of the mixed extract according to the present invention was determined using the inhibition concentration curve of FIG.

As shown in Table 2, the concentration (IC ₅₀ ) value of 50% inhibition of human whole blood agglutination of the mixed extract of Example 1 according to the present invention was 526.3 ㎍ / ㎖, Comparative Example 1 (5000 ㎍ / ㎖) Lower inhibitory concentrations were shown. From this, it was found that the mixed extract according to the present invention can effectively suppress whole blood aggregation even at a low concentration.

< Experimental Example  2> Platelet receptor  Expression inhibition effect measurement ( In vitro )

In order to determine the effect of inhibiting the expression of platelet receptors of the mixed extract according to the present invention, the following experiment was performed.

Platelet receptor expression corresponds to CD41 (Anti-Human Platelet GPIIb / IIIa Complex, Becton Dickinson), PAC-1 (type 1 PACAP receptor, Becton Dickinson), P-selectin (P-selectin, Becton Dickinson) Antibodies were used and tested using flow cytometry (Becton Dickinson). 90 µl of human whole blood was incubated at room temperature for 5 minutes, and each of 10 µl of phosphate buffered saline (PBS) and the mixed extract of Example 1 was added, and then adenosine diphosphate (adenosine diphosphate, ADP) was used as a platelet activity inducer. 10 μM was added and incubated for 2 minutes. 20 μl of a monoclonal antibody corresponding to each of CD41 and PAC-1 or CD41 and P-selectin was added thereto, followed by incubation at room temperature and in the dark for 20 minutes. 500 μl of 2% paraformaldehyde was added to stop the reaction, and then the degree of platelet membrane receptor expression was measured using a flow cytometer. It was measured by the value of% gated (degree of expression of PAC-1 or P-selectin in platelets expressing CD41) and expressed as the ability to inhibit platelet receptor expression by the extract. The results are shown in Table 3 below.

Inhibitory effect on the expression of platelet receptor of the mixed extract according to the present invention (% gated)
Platelet receptor
Phosphate-buffered saline
(Negative control)
Phosphate-buffered saline + ADP
(Positive control)
Example 1 (5000 μg / ml)
PAC-1
1.82 ± 0.89
91.82 ± 3.29
51.88 ± 29.28 (p <0.02)
P-selectin
2.13 ± 0.59
74.30 ± 7.76
48.56 ± 16.25 (p <0.02)

As shown in Table 3, in the positive control group administered with ADP as a platelet activator, it was found that the platelet membrane receptor expression of PAC-1 and p-selectin was increased. Membrane receptor expression was reduced. From this, the mixed extract according to the present invention was found to inhibit the platelet membrane receptor expression.

< Experimental Example  3> Intraplatelet  Calcium mobilization inhibitory effect measurement In vitro )

In order to determine the inhibitory effect of platelet calcium mobilization of the mixed extract according to the present invention, the following experiment was performed.

To test platelet calcium mobilization, fluorine-4AM (Fluo-4AM, Becton Dickinson) was used for calcium fluorescence staining, and flow cytometry (Becton Dickinson) was used for the measurement. Calcium fluorescence-stained fluoro-4AM was added to 1 ml of human platelet-rich plasma to a final concentration of 8 μM, and then incubated at 37 ° C. for 30 minutes. Phosphate-buffered saline, 20 μl of the mixed extract of Example 1 and 20 μl of the mixed extract of Comparative Example 1 were added to 180 μl of the culture solution, followed by incubation at 37 ° C. for 5 minutes, followed by addition of 300 μl of Tyros-Tyrode's buffer. 25 μl of ADP (final concentration 10 μM) was added thereto, and the degree of calcium activation was measured using a flow cytometer using a flow cytometer. The results are shown in Table 4 below.

Inhibitory Effect of Mixed Extract on Platelet Calcium Mobilization (MFI)
Measurement period
Control (PBS)
Example 1 (2.5 mg / ml)
Comparative Example 1 (2.5 mg / ml)
Reaction electricity
4.10 ± 0.10
4.00 ± 0.08
4.09 ± 0.09
Reaction maximum
8.78 ± 1.23
5.94 ± 0.19 (p <0.02)
8.19 ± 0.87
Reaction decay
6.75 ± 0.71
4.86 ± 0.30
6.70 ± 0.64

As shown in Table 4, the mixed extract of Example 1 showed a lower value than the MFI value shown in the reaction maximum of the control group. It was found that the mixed extract according to the present invention has a high inhibitory effect on platelet calcium mobilization in human platelet-rich plasma induced by platelet activator ADP.

< Experimental Example  4> blood flow Attachment  Suppression measurement ( In vitro )

In order to determine the inhibitory effect of hemocytosis induced by hemocytosis stress of the mixed extract according to the present invention, the following experiment was performed.

Blood flow chambers (live cells, Chamlide SC, Shear stress chamber) and syringe pumps (syringe pump, Harvard, KGS-101) were used to measure hemostatic stress induced by hemodynamic stress. A focus microscope (Confocal laser scanning microscope, Leica, TCSSP2) was used. The mixed extracts of Example 1 and Comparative Example 1 were added to 3 ml of human whole blood so as to have a final concentration of 2.5 mg / ml, and then reacted at room temperature for 5 minutes. The mixed solution was connected to a blood flow chamber tube containing a collagen coated slide, and then the flow rate of the syringe pump was adjusted to 0.428 ml / min for 4 minutes to pass. As a result, the blood cell strain stress rate of whole blood passing through the blood flow chamber tube was about 1300s ^-1 . After the reaction was completed, blood cells remaining on the collagen coated slides were washed by passing the thyroid buffer at the same flow rate. CCD camera images of the cells adhered to the collagen-coated slides in the blood chambers were taken by the CCD camera to visually determine the degree of inhibition of blood flow adhesion induced by hemocytosis stress. The results are shown in Fig.

As shown in FIG. 2, in the case of the sample treated with the mixed extract of Comparative Example 1, blood cells were attached to the collagen coating slide without significant difference from the control group. On the other hand, the sample treated with the mixed extract of Example 1 was found to significantly reduce blood cell adhesion. From this, it was found that the mixed extract according to the present invention can effectively inhibit the aggregation of platelets.

&Lt; Formulation Example 1 > Preparation of pharmaceutical preparation

1. Manufacture of rings

70 parts by weight of the mixed extract according to the present invention

20 parts honey

1 part by weight of starch

The ring was prepared using the conventional method with the said composition and content.

2. Preparation of Tablets

100 mg of the mixed extract according to the present invention

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the usual ingredients, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of Capsule

100 mg of the mixed extract according to the present invention

100 mg corn starch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

Preparation Example 2 Preparation of Beverage

1. Manufacture of carbonated beverages

5 to 10 parts by weight of sugar, 0.05 to 0.3 parts by weight of citric acid, 0.005 to 0.02 parts by weight of caramel, 0.1 to 1 parts by weight of vitamin C are mixed, and 79 to 94 parts by weight of the mixed extract according to the present invention are mixed to make a syrup. In addition, the syrup was sterilized at 85 to 98 ° C. for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 4, and 0.5 to 0.82 parts by weight of carbon dioxide was injected to prepare a carbonated beverage containing the composition of the present invention.

2. Manufacture of health drinks

79-90 parts by weight of subsidiary materials such as liquid fructose (0.5 parts by weight), oligosaccharides (2 parts by weight), sugar (2 parts by weight), salt (0.5 parts by weight), water (75 parts by weight) and mixed extracts according to the present invention After sterilization by the homogeneous formulation, it was packaged in small packaging containers such as glass bottles and plastic bottles to prepare healthy drinks.

3. Preparation of Vegetable Juice

5 g of the mixed extract according to the present invention was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice for health promotion.

4. Preparation of Fruit Juice

1 g of the mixed extract according to the present invention was added to 1,000 ml of apple or grape juice to prepare fruit juice for health promotion.

The mixed extract according to the present invention not only has an excellent inhibitory effect on whole blood coagulation ability, platelet membrane receptor expression, platelet calcium mobilization, etc., but also has an excellent effect on inhibiting blood flow adhesion induced by hemocytosis stress, such as atherosclerosis, cerebral hemorrhage, and stroke. , Cerebral infarction and the like can be usefully used for the prevention and treatment of diseases associated with blood circulation disorders.

Claims (9)

Pharmaceutical composition for the prevention or treatment of thrombotic diseases by improving blood circulation, which contains a mixed extract of maeyuk meat, sine, excess and sandalwood as an active ingredient. The method for preventing or treating thrombosis disease by improving blood circulation according to claim 1, which comprises 18 to 22 parts by weight of the falcon meat, 1 to 4 parts by weight, 1 to 3 parts by weight, and 1 to 3 parts by weight of sandalwood. Pharmaceutical composition for. 2. The pharmaceutical composition for preventing or treating thrombosis disease by improving blood circulation, according to claim 1, wherein the maeupuk, sine, excess and sandalwood are mixed extracts obtained by immersing in a polar solvent and heating. The pharmaceutical composition of claim 3, wherein the polar solvent is water, methanol, ethanol or a mixed solvent thereof. The method of claim 1, wherein the thrombotic disease is at least one selected from the group consisting of atherosclerosis, cerebral hemorrhage, stroke and cerebral infarction. Food composition for preventing or ameliorating thrombotic disease by improving blood circulation, comprising a mixed extract of maeyang meat, sine, excess and sandalwood and food supplements acceptable food. According to claim 6, 18 to 22 parts by weight of maeyuk meat, 1 to 4 parts by weight, excess 1 to 3 parts by weight and 1 to 3 parts by weight sandalwood prevention or improvement of thrombosis disease by blood circulation improvement Food composition. The food composition for preventing or improving thrombosis disease by improving blood circulation, according to claim 6, wherein the maeupuk, sine, excess and sandalwood are mixed extracts obtained by immersing in a polar solvent and heating. The method of claim 8, wherein the polar solvent is water, methanol, ethanol or a mixed solvent thereof, the food composition for preventing or improving thrombosis disease by improving blood circulation.

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