KR101280481B1 - Cosmetic for Improving Skin Wrinkle Comprising polyphosphate of fermented peppermint. - Google Patents

Abstract

The present invention relates to a functional cosmetic for improving skin wrinkles containing an inpolymer contained in the peppermint fermentation extract as an active ingredient, more specifically, after lyophilizing the peppermint immediately, hot water for about 2 hours at an extraction temperature of 100 to 200 ℃ Extracting and filtering, then concentrated under reduced pressure at 50 to 70 ℃ and purified and the peppermint fermentation extract prepared by the step of cultivating the obtained peppermint extract using Lactobacillus ashdophyllus to prepare a peppermint fermentation broth to cosmetics It relates to a functional cosmetics for improving skin wrinkles containing the phosphorus as an active ingredient contained in the peppermint fermentation extract to provide a cosmetic with excellent anti-wrinkle effect by high skin aging inhibition.
Functional cosmetics for improving skin wrinkles containing the inpolymer contained in the peppermint fermentation extract prepared through the present invention as an active ingredient is excellent in the effect of inhibiting skin aging can be usefully used for skin wrinkle improvement.

Description

Functional cosmetics for improving skin wrinkles containing phosphorus as an active ingredient in peppermint fermentation extracts {{Cosmetic for Improving Skin Wrinkle Comprising polyphosphate of fermented peppermint}}

The present invention relates to a functional cosmetic for improving skin wrinkles containing an inpolymer contained in the peppermint fermentation extract as an active ingredient, more specifically, after lyophilizing the peppermint immediately, hot water for about 2 hours at an extraction temperature of 100 to 200 ℃ Extracting and filtering, then concentrated under reduced pressure at 50 to 70 ℃ and purified and the peppermint fermentation extract prepared by the step of cultivating the obtained peppermint extract using Lactobacillus ashdophyllus to prepare a peppermint fermentation broth to cosmetics It relates to a functional cosmetics for improving skin wrinkles containing the phosphorus as an active ingredient contained in the peppermint fermentation extract to provide a cosmetic with excellent anti-wrinkle effect by high skin aging inhibition.

Skin elasticity is represented by elastic fibers composed of elastin in the dermis, which has a very low modulus of elasticity, such as rubber, which is easily deformed by small forces and easily removed when the forces are removed. Return to the circle.

In addition, elastic fibers have a form in which microfibrils are embedded in an amorphous matrix called elastin, which is a very unique feature found only in elastic fibers called desmosine and isodesmosine derived from lysine. A protein composed of amino acids.

Desmosin and isodesmosin form cross-links in long peptide chains, which make elastin rubbery. In addition, the elastic fiber composed of elastin is present along with collagen fibers called collagen, which is sufficient to maintain skin elasticity in a state where elastin and collagen are sufficiently present.

Decreased elasticity of the skin is caused by aging and degeneration of skin cells (fibroblasts) due to ultraviolet rays, etc., which causes elastin degradation and destruction. Elastin degrading enzymes are expressed in the skin by ultraviolet rays. It causes a decrease in skin elasticity.

Decreased skin elasticity is also caused by damage to and production of a protein called collagen, which makes up more than 70% of the skin dermis.

Collagen is also broken down by a degrading enzyme called collagenase, which is caused by ultraviolet light.

On the other hand, the protein fraction obtained from Leguminosae seed (El Patent No. 5,322,839) exhibits an elasticity increasing effect, the composition comprising malt extract (malt extract) and the like to inhibit the activity of collagenase (collagenase) It is applied.

Inhibitors of collagenase or elastinase include peptidyl carbamates, peptidyl thiocarbamates, hydroxamates, cephalosporins, and sulfonate salts. Cosmetic compositions (US Pat. No. 5,614,489) and the like have been reported.

However, in the conventional report, in order to show skin elasticity-enhancing effect, it merely inhibits the activity of collagenase or inhibits the activity of elastinase, and the effect is limited to the dermal layer.

Peppermint (Mentha arvensis var. Piperascens) described in the present invention is a perennial root of perennial herb of dicotyledons and moss plants. , Doucosterol, eugenol, linalol, menthone, (+)-neomenthol ((+)-neomenthol), and contraction of skin mucous membranes and blood vessels, It has the effect of numbing the nerves, stopping the pain, stopping the pruritus, giving the skin a unique refreshing feeling, improving skin disease and having a soothing and antioxidant effect.

Accordingly, the present invention has been proposed in view of the problems of the prior art as described above, and an object of the present invention is to provide a peppermint fermentation extract having a high anti-wrinkle effect due to high skin aging inhibition.

Another object of the present invention to provide a functional cosmetics for improving skin wrinkles containing the phosphorus as an active ingredient contained in the peppermint fermentation extract.

In order to achieve the object of the present invention,

Functional cosmetics for improving skin wrinkles containing the phosphorus as an active ingredient contained in the peppermint fermentation extract according to an embodiment of the present invention,

A lyophilization step of washing the mint to remove foreign substances, etc. present on the surface of the material, and then lyophilizing the mint;

Grinding the freeze-dried peppermint into a powder state of 50 mesh or more;

Putting the powdered mint in distilled water and extracting hot water for about 2 hours at an extraction temperature of 100 to 200 ° C .;

Concentration of the filtered peppermint extract under reduced pressure at 50 to 70 ° C. to obtain a final concentrate;

It is prepared using the peppermint fermentation extract prepared by culturing the obtained peppermint extract using Lactobacillus ashdophyllus to prepare a peppermint fermentation broth.

As described above, the peppermint fermentation extract of the present invention has an excellent effect of inhibiting skin aging, and thus may be usefully used for skin wrinkle improvement.

1 is a view showing the cell regeneration effect.
2 shows the results of the protease inhibition test (MMP-2).
3 shows the results of the protease inhibition test (MMP-1).

Functional cosmetics for improving skin wrinkles containing the phosphorus contained in the peppermint fermentation extract of the present invention for achieving the above object,

A lyophilization step of washing the mint to remove foreign substances, etc. present on the surface of the material, and then lyophilizing the mint;

Grinding the freeze-dried peppermint into a powder state of 50 mesh or more;

Putting the powdered mint in distilled water and extracting hot water for about 2 hours at an extraction temperature of 100 to 200 ° C .;

Concentration of the filtered peppermint extract under reduced pressure at 50 to 70 ° C. to obtain a final concentrate;

It is prepared using the peppermint fermentation extract prepared by culturing the obtained peppermint extract using Lactobacillus ashdophyllus to prepare a peppermint fermentation broth.

At this time, the content of the peppermint fermentation extract,

It is characterized in that 0.001 to 50 parts by weight based on the total weight of the cosmetic.

At this time, the functional cosmetics for improving skin wrinkles containing the phosphorus contained in the peppermint fermentation extract as an active ingredient,

It is characterized by having a formulation selected from the group consisting of nutritive cosmetics, creams, essences and softening cosmetics of solubilized formulations.

At this time, the functional cosmetics for improving skin wrinkles containing the phosphorus contained in the peppermint fermentation extract as an active ingredient,

The polymer is 0.0001 to 10 parts by weight based on the total weight of the cosmetic.

At this time, it is characterized in that it has a formulation selected from the group consisting of nutrient cosmetics, creams, essences and softening of the solubilized formulation of the emulsion formulation containing 0.0001 to 10 parts by weight of the phosphorus contained in the peppermint fermentation extract.

Of note in the cosmetics industry are wrinkle improvement, whitening and sun protection.

This is a situation where various natural products are used.

Example 1 Physicochemical Properties of Peppermint Fermented Extract

Peppermint fermentation extract was prepared as an aqueous solution to measure specific gravity, refractive index, dry residue, pH, heavy metal (lead), arsenic content.

This is an item to be equipped when selling as a cosmetic raw material using peppermint fermentation extract, and among these, heavy metal and arsenic content are the most important items.

Specific gravity (DFT) refers to the weight ratio of the same volume at t '℃ and t ℃ between the sample and water, respectively.

The specific gravity scale was measured at the top of the meniscus when the specific gravity liquidimeter was stopped using the measurement method of the specific gravity liquidimeter, and the hydrometer was measured using the hydrometer of the Korean weighing machine.

The refractive index of the material is the ratio of the speed of light in the vacuum to the speed of light in the material and the ratio of the sine of the angle of incidence of the light to the material and the sine of the angle of refraction.

Usually the refractive index changes with the wavelength and temperature of the light.

The refractive index n tD refers to the refractive index with respect to air when measured at the temperature t ° C. using the D line of sodium spectra as a light ray.

In this experiment, it was measured at 20 ℃ using NAR-1T (ATAGO CO.).

The ignition residue test is usually applied to determine the content of inorganic matter as an impurity in organic matter, but sometimes to measure the amount of impurities in inorganic or volatile inorganic matter as a constituent of organic matter.

However, in this experiment, the harsh conditions were not performed at 500 ° C. or higher, and the residual residue was measured by heating at 105 ° C. for 2 hours. (Mettler LP16, TOLEDO Co.)

pH measurement was performed using a standard buffer solution using a pH meter with a glass electrode, and the value was accurate to within ± 0.02. (1% Soln., Model 420, ThermoOrion Co.)

Arsenic test means a limit test of arsenic contained as impurities in raw materials.

The limit is expressed in terms of arsenic trioxide (As203), usually expressed in parts per million (ppm).

The heavy metal test is a limit test for heavy metals contained as impurities in raw materials.

Heavy metals refer to metallic mixtures that are acidic to sodium sulfide solution, the limits of which are expressed in parts per million (ppm) as lead (Pb).

Physical properties Experiment Item mint importance 1.007 Refractive index 1.346 Ignition residue (%) 1.4 pH (1%) 5.39 Arsenic (ppm) LOQ Lead (ppm) LOQ

The limit of quantification (LOQ), that is, when measured as a solution, shows <7.0 ppb for pb and <11.0 ppb for As.

That is, the content of the most important heavy metals or arsenic of the experimental items was detected in an extremely low amount.

Example 2 Primary skin irritation test of peppermint fermentation extract

Dermal primary irritation refers to acute inflammation or skin necrosis caused by a single contact of a substance that irritates the skin. The primary irritation does not cause inflammation, but when caused by repeated contact, it is called a cumulative irritation.

There are many substances that can cause primary irritation, which is indicated by one-time application of strong irritant substances to the skin and used as a means of predicting stimulation or cumulative stimulation in human testing.

In general, the primary stimulation method is a variation of the Draize method.

Tests are not carried out for pH 2 or below 11.5 or above for peppermint fermentation extracts, and for skin LD50 of 200 mg / kg or more.

Rabbits were chosen as test animals, and rabbits are used for the development of new pharmaceutical and cosmetic raw materials and are rich in basic physiological and anatomical toxicological data.

The age was 6 weeks and 12 animals were tested.

During the seven days of quarantine and adaptation, weight gain and general symptoms are examined during this period.

About 24 hours before application of the test substance, the hair of the rabbit's hair is removed by a hair removal machine so that it does not hurt so that it is more than 10cm long and vertical.The test animal is about 20mm x on the right side of the back by using an abrasion tape on the back. Make a 20mm-sized scratch and site and test it by placing the mirror and site so that it is symmetrical.

Apply 0.1 ml of the test substance to the patch test tape, and then attach the tape to the rub, non-crumb and areas.

The tape attachment site is fixed with paper tape to prevent it from falling off, and then the entire test site is fixed with an elastic band and maintained in the calibration frame for 24 hours.

After 24 hours, the patch is removed and washed with physiological saline so that no test substance remains.

In the case of a single concentration administration, 0.5 ml or 0.5 g is used as a dose.

24 hours and 72 hours after application, the presence of irritation such as erythema and skin formation and edema formation is evaluated.

The result of skin reaction of each animal is evaluated by the score according to the evaluation criteria of skin response of toxicity test standard of medicines.

The skin primary irritation index (P.I.I) is calculated from erythema and edema grades at 24 and 72 hours after application of the test substance.

The mean value of erythema grade and edema grade in each of the abrasion site, the non-brain site, and the average area of the results at 24 hours and at 72 hours are used as the primary skin irritation index.

Evaluation of skin reactions (sum of erythema and dermal and graded edema) Erythema and scab formation score No erythema 0 Very mild erythema (only visible to the naked eye) One Pronounced erythema 2 Severe erythema 3 Severe erythema (severe redness) and crust formation 4 Edema formation score No edema at all 0 Very mild edema, barely visible to the naked eye One Mild swelling (if the swelling is distinct and the margins are distinct) 2 Severe edema (when swelling about 1 mm) 3 Severe edema (swelling more than 1 mm and extending beyond the exposed area) 4

 Result judgment list by skin primary stimulation index Stability Category Skin Primary Stimulation Index (P.I.I) Judgment 0.0 to 0.5 No stimulation Available 0.6-2.0 Light stimulus Concentration adjustment 2.1 ~ 5.0 Moderate irritation can not be used 5.1 and above River stimulation can not be used

Skin Primary Stimulation Index Results of Peppermint Fermented Extracts Number of test sites Test concentration (%) Vehicle P.I.I Judgment Remarks 12 10 water 0.094 No stimulation Available

As shown in Table 4, there were no rabbits showing unusual clinical symptoms or death during the experiment, and the P.I.I of the peppermint extract was 0.188, corresponding to the non-irritating range.

In addition, there was pigmentation by the substance itself at 10% concentration, but not enough to affect the stimulus evaluation.

Example 3 Ocular Mucosal Stimulation Test of Peppermint Fermentation Extracts

Because of the potential for eye contact, eye mucosal irritation tests are performed.

Evaluation of the irritation of the ocular mucosa response of each animal is evaluated using the grade of eye lesions according to the toxicity test criteria, such as pharmaceuticals.

The age was 6 weeks and 18 animals were tested.

Ocular Membrane Stimulation Test of Peppermint Fermented Extracts Number of test sites NW ^{A )} / W ^{B )} Test concentration (%) Vehicle 4/2 10 Water

NW ^{A )} is non-washing and W ^{B )} means washing.

At 1, 24, 48, and 72 hours after administration of the extract, the application site of the test substance was observed. Observed.

Eyedrop irritation test results of mint fermented extract Rating
Evaluation value AOI ^{a )} MOI ^{b )} Day 7 IOI ^{c )} Non irritant 0 to 1 0 (after 48hrs) practically non irritant 1 to 5 ≤1 (after 48hrs) Minimally irritant 5 to 15 ≤5 (after 48hrs) Midly irritant 15 to 30 ≤5 (after 4days) Moderately irritant 30 to 60 ≤20 (after 7days) ≤30 (tested animals all) Severely irritant 60 to 80 ≤40 (after 7days) ≤60 (tested animals all) Extremely irritant 80-100

AOI ^{a )} (Acute ocular irritation index): Max. Among MOI

MOI ^{b )} (Mean ocular irritation index): Total score / tested animal NO. In each observation time

Day 7 IOI ^{c )} (Individual ocular irritation index): score of each animal on Day-7

Eye Membrane Stimulation Test Results of Peppermint Fermented Extracts Irritation Result: MOI ^{a )} (NW / W) AOI ^{b )} 1 hours 24 hours 48 hours 72 hours 0.5 / 0.5 0/0 0/0 0/0 0.5

MOI ^{a )} (Mean ocular irritation index): Total score / tested animal NO. In each observation time

AOI ^{b )} (Acute ocular irritation index): Max. Among MOI

The stimulus result (AOI ^{b )} was 0.5, indicating a practically non irritant.

Thus, it was found that the extract can be used.

Example 4 Single-Dose Oral Test of Peppermint Fermentation Extracts

Species and strains are ICR mice, 4-5 weeks old, with 5 females and 5 males.

Single dose oral test test group of peppermint fermentation extract Dose gender Number of test animals Route of administration Vehicle 2 g / kg
female 5 oral- Water
cock 5 oral-

Single dose oral test results of peppermint fermented extract Vehicle Dos gender Mortality LD50 Water
2 g / kg
female 0/5 2 g / kg or more cock 0/5 2 g / kg or more

As described above, no abnormal symptoms caused by single dose oral toxicity were not observed, and it was found that LD50 was a safe extract in terms of single dose oral toxicity at 2 g / kg or more.

Example 5 Analysis of Cell Regeneration Effect of Peppermint Fermentation Extract

In order to find out whether the peppermint fermentation extract of the present invention has an anti-wrinkle effect, the effect of the peppermint fermentation extract on the skin cells by concentration was analyzed.

Specifically, human fibroblasts (HNF, Normal Human) in a T-75 flask

Fibroblast) (CCD-986K, KCLB 21947) was incubated with 1 × PBS 2-3 times, and treated with 0.025% Trypsin-EDTA to drop the cells attached to the flask.

The cells were obtained and centrifuged at 1500 rpm for 5 minutes (Heraeus, Labofuge 400R), and then pelleted cells were suspended in 10 ml of DMEM (including 5% FBS). After measuring the number of cells by using a hematocytometer (Heamacytometer), the cells of about 2 × 103 was dispensed into each well of 200 well each 96 well plate.

When cells grow to about 50% of the well area, the peppermint fermentation extract of the present invention is added to each well at concentrations of 1,000, 500, 250, 125, and 62.5 ppm while exchanging with fresh DMEM (containing 2% FBS) medium. Cells were incubated at 37 ° C., 5% CO 2.

Three days after incubation with the addition of the extract of the present invention, MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide) analysis was performed to determine the regeneration effect of the cells.

MTT (Sigma) was dissolved in PBS at a concentration of 2 mg / ml, 10% (20 μl) of the cell culture medium was treated, and the cells were further incubated for 2 hours.

After discarding the supernatant of the cultured cells, add 150 μl of DMSO and mix well or add 100 μl of 0.04 N HCl (in acid-isopropanol) and mix.

After the procedure, the absorbance was measured at 560 nm using an ELISA reader (μQuant, Bio-Tek instrument INC.) (Reference: 650 nm).

Cell Regeneration Effect of Peppermint Fermented Extract Concentration (ppm) 1,000 500 250 125 62.5 mint 116.0634606 133.5151215 150.5205751 147.8929103 149.231532 Vigor 127.7864017 134.2026286 127.9933768 111.9528097 110.8661906 Digression 86.74127848 80.7420851 82.55696713 73.98669087 79.58257713 10% FBS 161.2731609

As shown in Figure 1, it was found that there is little cell regeneration effect in the case of sediment, the mint showed a regeneration effect of 30 to 50% at a concentration of 500 ~ 62.5ppm, 500 ~ 62.5 in the case of vigor Regeneration effect of 10-30% was shown at the concentration of ppm.

This is an unparalleled proliferative effect compared to other substances that have the effect of proliferating fibroblasts by 20-30%.

Example 6 Analysis of Protease Inhibitory Function of Peppermint Ferment Extract

An experiment was performed using matrix metalloproteinase (MMP), an activated protease that degrades collagen, a major component of the extracelluar matrix.

Western blot and immunoassay methods are used to confirm the expression level of collagenase by antigen-antibody reactions of proteins.

The method was to look at the expression level (MMP-1) and, as well as these, it was necessary to confirm the activity of the protein was tested by the zymography method (MMP-2).

In other words, the effect of the extract on the decrease of procollagen and the increase of MMP expression by UVA after UVA was injected into Normal Human Fibroblast.

Adjust to load the same amount of protein, mix the same volume of sample buffer (2X) of the sample of the present invention and leave for 10 minutes at room temperature.

Load into the well using a pre-casting gel, and run the gel for about 90 minutes at 125v constant voltage.

Subsequently, in a 100ml Novex Zymogram Renaturing Buffer (1X), gently shake the gel of one or both plates for 30 minutes.

Change to Novex Zymogram Developing Buffer (1X) and shake for about 1 hour.

Change to a new Developing Buffer and leave at 37 ° C overnight.

Stain with Commassie Blue R-250 solution (dye 50 mg, 22.5 ml MeOH, 22.5 ml DIW, 5 ml glacial acetic acid).

Decolorize with destaing solution (5 ml of MeOH, 5 ml of glacial acetic acid, 40 ml of DIW).

Use a dry kit to dry carefully to avoid bubbles.

As shown in Figure 2, MMP-2 (Zymography) experiments as compared to the control sample was confirmed that all the samples (mint, vigor, degeneration) in the MMP2 expression and activity reduction is ineffective.

As shown in Figure 3, MMP-1 expression and procollagen experiments were confirmed that peppermint has an effect of inhibiting the expression of MMP-1 by UVA, procollagen was not effective in all samples.

Meanwhile, polyphosphate refers to a linear polymer of phosphates in which two or more phosphates are connected to dozens or hundreds of phosphates to form orthophosphate (Pi), and each bond of the phosphate molecule is a high energy anhydrous phosphate ( phosphoanhydride).

The chain ends of these inpolymers are attacked by AMP, ADP, glucose or water, respectively, by the catalysis of P-AMP phosphotransferase, inpolymer kinase, inpolymer glucokinase and exopolyphosphatase, and internally. Is attacked by endopolyphosphatase.

Since these inpolymer molecules have a high anion state, they can bind firmly to polyvalent cations such as cobalt, copper, magnesium, calcium, iron, and zinc. Due to this property, substances to be taken through normal microbial metabolism can be stably bound. It is known that it can eventually inhibit the growth of microorganisms.

Phosphorus polymers are used in food and cheese color promoters and additives, canned milk curds, various carbonated water additives, hemorrhoids and gastrointestinal drugs, and have been certified as harmless to the human body by the US FDA and the Department of Agriculture.

Vitamin C, vitamin E, and the like, which are commonly used as antioxidants, have limitations such as discoloration due to water or light in various formulations, and their amount of use has been extremely limited.

Phosphorus polymers can be usefully used in various antioxidant cosmetics because they can overcome the limitations of the formulations of the conventional antioxidants.

In addition, in the functional cosmetics for improving skin wrinkles containing the phosphorus polymer contained in the peppermint fermentation extract of the present invention as an active ingredient, the phosphorus polymer has a high antioxidant activity and excellent anti-oxidation inhibitory activity and radical scavenging activity, It can be usefully used to prevent peroxidation.

Therefore, the inpolymer may be used as a cosmetic sample for inhibiting skin aging, maintaining skin elasticity or improving wrinkles, and is preferably used as a basic cosmetic sample of softening cream, nutrient cream, nourishing cream, essence, pack or bath powder. Can be.

In functional cosmetics for improving skin wrinkles containing an inpolymer contained in the peppermint fermentation extract of the present invention as an active ingredient, 0.0001 to 20 parts by weight, preferably 0.001 to 10 parts by weight of the inpolymer in the cosmetic composition Is added in amounts.

As shown in Table 11, it was confirmed that the mint of the present invention contained more polyphosphate than other herbal medicines.

Phosphorus content of mint Chinese medicine name Dry weight (g / 200g) Recovery rate (%) OD value (530/630) PolyP amount (ug / ml) mint 28.67 14.335 0.0916 0.293 Upper lobe 30.9 15.45 - - Chrysanthemum - - - - Changwon 59.68 29.84 - - Shiho - - - - riding - - - - Vigor 54.27 27.135 0.0195 0.0307 Digression 1.85 0.925 0.0033 1.757

On the other hand, it is shown that the content of the inpolymer is higher than the mint, but in the cell regeneration effect as shown in Figure 1 is lower than the mint in the case of manufacturing cosmetics using the inpolymer of the mint as an active ingredient much better than the selection Cell regeneration efficacy is good.

Example 7 Preparation of Peppermint Fermentation Extract Using Lactobacillus Ashdophilus

After washing to remove foreign substances present on the surface of the mint, the freeze-dried peppermint is immediately dried, and the freeze-dried peppermint is pulverized. 20 g of the ground peppermint is put in 400 ml of distilled water and hot water at 200 ° C. for 2 hours. Extracted and filtered.

The filtered peppermint extract was concentrated under reduced pressure at 50 to 70 ° C., the final concentrate was adjusted to 40 ml, and then filtered to prepare a peppermint extract.

The extracted peppermint extract was added to the Lactobacillus acidophilus culture to 10g / L.

Dextrose as a carbon source and dipotassium phosphate as a pH adjuster were added thereto.

The strain used was Lactobacillus acidophilus (Lactobacillus acidophilus) was used, and was added at 50,000 cfu / L per culture.

The culture was carried out using a fermenter for 10 days, maintained at 35 ℃, pH 5-7.

After incubation, the culture solution was centrifuged to remove the culture cells first, and then filtered through a filter.

The filtrate thus obtained is aged again for about 10 days in a low temperature warehouse at 4 ° C., and the filtrate is filtered again using a filter to obtain a final peppermint fermentation broth, which is used in the manufacture of cosmetics.

Preparation Example 1 Preparation of Cosmetics Using Mint Fermented Extract

Functional cosmetics for improving skin wrinkles containing the inpolymer contained in the peppermint fermentation extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation.

In a preferred manufacturing example of the present invention using the peppermint fermentation extract was prepared cosmetics of solubilized formulations, such as cosmetics, emulsified cosmetics, such as nutrient cosmetics, creams, essences, emulsion formulations.

Emulsified formulations were prepared to contain 50 parts by weight of peppermint fermentation extract based on the total weight of cosmetics, and solubilized formulations were prepared to contain 20 parts by weight of peppermint fermentation extract relative to the total weight of cosmetics. Since it is excellent in the effect of protecting collagen (particularly, the effect of suppressing inflammation), it is preferable to maintain the above-mentioned parts by weight.

<1-1> Cosmetic Preparation of Emulsifying Formulations

The production method is as follows.

1) 0.3 parts by weight of stearic acid, 0.2 parts by weight of steali alcohol, 1.2 parts by weight of glyceryl monostearate, 0.4 parts by weight of beeswax, 2.2 parts by weight of polyoxyethylene sorbitan monolauric acid ester, 0.1 parts by weight of methyl paraoxybenzoate, The primary mixture of 0.05 parts by weight of paraoxybenzoic acid profile, 5 parts by weight of cetylethylhexanoate and 2 parts by weight of triglyceride was heated to 60 to 80 ° C.

2) Then, 3 parts by weight of cyclomethicone was added to the primary mixture to prepare a secondary mixture.

3) A mixture of 100 parts by weight of distilled water, 5 parts by weight of concentrated glycerin, and 0.15 parts by weight of triethanolamine is heated to 65-70 ° C. to undergo a dissolution step for complete dissolution.

4) During the dissolution step, the secondary mixture of 2) was slowly added to emulsify for 2-3 minutes at 8,000 rpm.

5) 0.12 parts by weight of a polyacrylic acid polymer was dissolved in water, and then added to the mixture of 4) and emulsified for 2 minutes.

6) 0.0001 parts by weight of pigment, 0.1 parts by weight of fragrance, 50 parts by weight of peppermint fermentation extract was added to the mixture of 5) and emulsified for 1 minute.

7) By cooling the mixture of 6) through a degassing process and then cooled to 25 ~ 35 ℃ to prepare a cosmetic having a high wrinkle-improving effect of the emulsifier type.

<1-2> Cosmetic preparation of solubilized formulation

The production method is as follows.

1) 3 parts by weight of concentrated glycerin, 2 parts by weight of 1,3-butylene glycol, 0.01 part by weight of EDTA-2Na, 0.0002 part by weight of pigment, and 20 parts by weight of peppermint fermented extract were added to 100 parts by weight of purified water and dissolved using a still mixer. .

2) 0.1 part by weight of methyl paraoxybenzoate, 0.3 part by weight of polyoxyethylene hydrogenate ester, 0.15 part by weight of fragrance, and 0.2 part by weight of cyclomethicone were added to 8 parts by weight of alcohol (95%) and completely dissolved.

3) The mixture of 2) was solubilized by slowly adding to the mixture of 1) to prepare a cosmetic having a high anti-wrinkle effect of the solubilized formulation.

It will be appreciated by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. Therefore, the above-described embodiments are to be understood as illustrative in all respects and not restrictive.

The scope of the invention is indicated by the following claims rather than the above description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the invention. do.

Claims (5)

A lyophilization step of washing the mint to remove foreign substances, etc. present on the surface of the material, and then lyophilizing the mint;
Grinding the freeze-dried peppermint into a powder state of 50 mesh or more;
Putting the powdered mint in distilled water and extracting hot water for about 2 hours at an extraction temperature of 100 to 200 ° C .;
Concentration of the filtered peppermint extract under reduced pressure at 50 to 70 ° C. to obtain a final concentrate;
Cultivating the obtained peppermint extract using Lactobacillus ashidophilus to prepare a peppermint fermentation broth; wherein the content of the peppermint fermentation extract is
Functional cosmetics for improving skin wrinkles containing the phosphorus as an active ingredient contained in the peppermint fermentation extract, characterized in that 0.001 to 50 parts by weight based on the total weight of the cosmetic.
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