KR101189141B1 - Method for preparing fiber of Aloe vera Linne parenchyma tissue for the dietary fiber - Google Patents

Abstract

The present invention relates to a method for producing fiber of aloe vera oil cell tissue for dietary fiber, washing with fresh mature aloe vera to remove adherent soil and other deposits, and then peeling to remove alloin and gel of the cell tissue. After obtaining, centrifugation (8000 xg) to separate and fractionate the fiber and gel of aloe vera, fractions of the aloe fibres are taken, lyophilized and powdered. As a fiber of aloe vera oil cell tissue, swelling power, water retention capacity and fat absorption capacity are better than known alpha-cellulose dietary fiber, and it does not show foaming ability, and thus foam stability is high, and emulsification capacity and emulsion stability are also excellent. In addition, the CMC-containing solution has a very good dispersibility, exhibits higher concentration dependence and pseudoplastic viscosity characteristics than alpha-cellulose at a concentration of 1% or more, and thus can be usefully used as a new dietary fiber material. Excellent absorption delay effect, has the effect of improving obesity and constipation.

Aloe Vera, Aloe Vera Fiber, Aloe Vera Cellulose, Dietary Fiber

Description

Method for preparing fiber of Aloe vera oil cell tissue for dietary fiber {Method for preparing fiber of Aloe vera Linne parenchyma tissue for the dietary fiber}

The present invention relates to a method for producing fiber of aloe vera oil cell tissue for dietary fiber, and more specifically, to wash adherent soil and other adherents by washing with freshly mature fresh aloe vera, and then peeling to remove aloin and After obtaining a gel of the cell tissue, centrifugation (8000 xg) to separate and fractionate the fiber and gel of aloe vera, fractionate, take the aloe fiber fraction, lyophilized, and then obtain a powder of the powdered aloe vera cell tissue. It has better swelling power, water holding capacity and fat absorption ability than known alpha-cellulose dietary fiber, high foam stability because it does not show foaming ability, excellent emulsifying and emulsifying stability, and CMC-containing solution is very good. It has excellent dispersibility and shows higher concentration dependence and pseudoplasticity than alpha-cellulose at the concentration of 1% or more, so it can be usefully used as a new dietary fiber material, and has excellent effect of delaying glucose absorption and delaying absorption of bile acid. There is an improvement in obesity and constipation.

Aloe belonging to the family Liliaceae is a perennial plant, CAM (Crassulacean Acid Metabolism) plant with succulent fleshy leaves and has been widely used as a folk remedy extensively for more than 3500 years (Reynolds and Dweck, 1999).

More than 400 species of aloe are known, including Aloe vera ( Aloe) vera Linne), aloe aborescens and aloe saponaria are mainly known. Aloe vera juice or gel is one of the representative health functional foods and is one of the most widely used natural materials as a pharmaceutical or cosmetic ingredient (Kawai et al. 1993; Eshun and He, 2004).

Aloe vera is a raw ingredient that has consistently maintained the top ten position in various food and cosmetic formulations over the past two decades, and its use has increased in recent years, resulting in more than 1500 final products containing aloe. It is known to become (www.iasc.org).

The internal parenchyma tissue of the aloe vera leaves contains more than 99% water, and polysaccharides make up the bulk of the dry weight of the aloe vera flow tissue (Grindlay and Reynolds, 1986; Reynolds and Dweck, 1999). In addition, the fiber in the flow cell tissue occupies about 10% of the gel and is the next-largest component after the water content, especially as a by-product of processing, although the characteristics as a dietary fiber are expected to be great, but its role and aptitude are not clearly identified at all. It has been scrapped.

Dietary fiber is a structural residue of plant cells that is indigestible by human digestive enzymes and has long been perceived to be of no nutritional value because it is not digested or absorbed. However, in the 1970s, Trowell (1972, 1976) and Burkitt et al. (1974) reported that diseases such as heart disease, bowel disease, obesity, diabetes, gallstones, and the like were associated with lack of dietary fiber. Since then, research and interest in the physiological role has been greatly increased (Schneeman, 1986, 1989; Reiser, 1987).

Dietary fibers are mainly polysaccharides and lignin, which are largely divided into insoluble and water-soluble dietary fibers, and their physiological effects have been reported to be different (Kay, 1982; Schneeman, 1987; Prosky, et al. , 1988; Lee and Lee). , 1996).

Water-soluble dietary fiber is mainly contained in fruits, barley, legumes, foods, water retention capacity (water-holding capacity), the gel (gel) ability to improve the viscosity of the food increases. In addition, it increases the residence time in the stomach, provides a feeling of satiety, slows the absorption of nutrients (Krotkiewski, 1987), and the delay in digestion and absorption of these nutrients increases the glucose tolerance in the blood of diabetics. Have been reported (Jenkins et al ., 1976, 1978; Anderson and Chen, 1979). In addition, by binding to bile acid (bile acid) is excreted in the feces to induce the consumption of cholesterol for bile acid supplementation in the body (Anderson et al. , 1984; Van Horn et al. , 1986). This has been reported to lower blood cholesterol levels, prevent heart disease and atherosclerosis, and lower the incidence of colorectal cancer (Koseki, et al. , 1990; Kay and Truswell, 1977).

On the other hand, insoluble dietary fiber is mainly composed of plant cell wall, which is contained in wheat bran, corn bran, and grains, and has a large water retention ability to give satiety on the stomach, and to thin the stools, reducing the travel time in the intestine, thereby facilitating bowel movement. (Anderson et al ., 1984). In addition, it is partially decomposed by bacteria in the large intestine and fermented into short chain fatty acids, and thus mainly shows effects on the large intestine function (Van Horn et al. , 1986). In addition, it is well known that dietary fiber has a blood pressure-lowering effect, is good for obesity, and is particularly helpful for the treatment of diabetes by delaying glucose absorption and reducing insulin demand (Koseki et al. , 1990; Yoon et al. ., 1987;. Torsdottir et al , 1990;. Kim et al, 2004).

Therefore, there have been studies to clarify various physiological functions through animal and clinical experiments for various natural sources of dietary fiber, and at the same time, as a representative characteristic of dietary fiber related to these physiological functions, water retention capacity and sol formation ability Extensive research has been done on physicochemical properties such as cation exchange capacity, bile acid binding capacity (Ebihara and Kiriyama, 1990; Eastwood et al. , 1986).

However, aloe vera fiber has now been discarded as a by-product of processing at all aloe processing companies and has not yet been considered as a dietary fiber.

Therefore, the object of the present invention is that the swelling power, water retention capacity and fat absorption ability are better than the known alpha-cellulose (α-cellulose) dietary fiber, it does not show the foaming ability, the foam stability is high, and the emulsification capacity and the emulsion stability is also excellent. , CMC-containing solutions have very good dispersibility, and have higher viscosity dependence and pseudoplasticity than alpha-cellulose at concentrations of 1% or more, and thus can be usefully used as a new dietary fiber material. The present invention provides a method for producing fiber of aloe vera oil cell tissue for dietary fiber, which has excellent delaying effect and has an effect of improving obesity and constipation.

In order to easily achieve the above object as well as other objects that are easily expressed in the present invention, after washing with aloe vera and peeling to remove the aloe and to obtain a gel of the flow cell tissue, centrifugation (8000 xg) aloe vera The fibrous and gel were separated and fractionated. The aloe fibrous fraction was taken, lyophilized, and powdered. By using fiber of aloe vera oil cell tissue as dietary fiber, swelling power, water retention capacity and fat absorption capacity are better than known alpha-cellulose dietary fiber, it does not show foaming ability, high foam stability, emulsification capacity and Emulsification stability is excellent, CMC-containing solution has a very good dispersibility, has a higher viscosity-dependent viscosity and pseudoplasticity than alpha-cellulose at a concentration of 1% or more, can be useful as a new dietary fiber material, glucose The absorption delay effect and the bile acid absorption delay effect was excellent, and the effect of improving obesity and constipation was obtained.

The fiber of the aloe vera oil cell tissue for dietary fiber according to the present invention is superior to the known alpha-cellulose dietary fiber, and it does not show the foaming ability by using the fiber as a dietary fiber. It has high stability, excellent emulsifying and emulsifying stability, and CMC-containing solution has a very good dispersing ability, and shows higher concentration dependence and plasticity of viscosity than alpha-cellulose at a concentration of 1% or more. It can be usefully used, excellent in delaying glucose absorption and delaying bile acid absorption, and improving the effect of obesity and constipation.

In the fiber manufacturing method of aloe vera oil cell tissue for dietary fiber according to the present invention, after washing with fully mature fresh aloe vera to remove adherent soil and other adherents, it is peeled to remove alloin and to obtain gel of milk cell tissue. After centrifugation (8000 xg), the aloe vera fibers and gels were separated and fractionated. The aloe fibrous fractions were taken, lyophilized and then powdered.

In the present invention, domestic aloe vera ( Aloe vera Linne) grown directly on the applicant's farm was used, and used while preserving in a low temperature room at 4 ° C.

First, mature and fresh aloe vera is screened, washed 2-3 times to remove adherent soil and other deposits, and then peeled to remove aloin.

Aloe is a bitter yellow fluid that is extracted from aloe. It is anthraquinone glycosides and is contained in the skin of aloe. Get the gel.

The gel of the aloe vera flow cell tissue obtained above is centrifuged to separate and fractionate the fiber and gel of the aloe vera. The aloe fiber fraction is taken, lyophilized, and powdered.

Centrifugation is preferably carried out under conditions in which fibers and gels are easily separated. In the present invention, centrifugation is performed at a rotational speed of 8000 xg, but the present invention is not limited thereto. There is a disadvantage that the equipment must be used, there is a problem that if the rotational speed is lowered, the centrifugation takes a lot of time and the centrifugation is not easy.

In addition, lyophilization of the fibrous fraction of aloe vera flow cell tissue is effective, and if the fibrous fraction of the aloe vera flow cell tissue is not altered, it may be dried using a drying method other than lyophilization. Although not limited, it was effective in the present invention for 24 hours at 0.05 torr.

The fiber of the dried aloe vera oil cell tissue is ground and powdered. In the present invention, the particle size of the powder during grinding is effective to about 80 mesh, but is not necessarily limited thereto.

The fiber of the aloe vera oil cell tissue for dietary fiber according to the present invention prepared as described above is superior to the known alpha-cellulose dietary fiber with swelling, water retention, and fat absorption ability by using as a fiber. It shows no foaming ability, high foam stability, excellent emulsifying and emulsifying stability, and CMC-containing solution has very good dispersing ability. It shows higher concentration dependence and pseudoplasticity than alpha-cellulose at 1% or higher concentration. It can be usefully used as a new dietary fiber material, excellent in delaying glucose absorption and delaying bile acid absorption, and improving obesity and constipation.

The following Examples and Experimental Examples illustrate the present invention in more detail, but do not limit the scope of the invention. In Examples and Experiments, alpha-cellulose (α-cellulose: product No. C8002, manufactured by Sigma-Aldrich, Inc.) was used as a comparative standard sample.

Example 1

Moisture, protein, fat, carbohydrate and ash content of fiber of Aloe Vera cell tissue were measured according to the conventional method, and the content of soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) was measured by AOAC. Prosky e t al. , (1988), and the total dietary fiber (TDF) content was calculated from the sum of insoluble fiber and water soluble fiber (TDF,% = IDF + SDF) and the results are shown in Table 1 It is described in.

Polysaccharides were also developed for the determination of glucomannan, the active ingredient index of aloe, Ebarandu et al. According to the colorimetric method of, (2005), it was measured as follows. That is, 400 μl of sample was transferred to a disposable glass culture tube, and 4 ml of Congo red (Congo red: sodium 4,4'-diphenyl-2,2`-diazo-bis-1-naphthalamino-4-sulfonate) was placed in each tube. A) reagent was added, gently vortexed and mixed, the mixture was left at room temperature for 20 minutes, and its absorbance was measured at 540 nm.

 At this time, the content of the polysaccharide was calculated from a standard curve prepared by separately preparing a standard polysaccharide and using the same.

Item (%, d.b.) moisture 1.13 ± 0.03 Crude protein 3.93 ± 0.05. Crude fat 0.31 + 0.02 Total ash 5.38 ± 0.10 carbohydrate 1.76 ± 0.05 Insoluble Dietary Fiber 67.90 ± 1.04 Water soluble fiber 19.59 ± 0.13

As can be seen from Table 1, the water content of the fiber of the aloe vera oil cell tissue was 1.13%, the remaining components of the carbohydrate content was the highest as 89.25% by dry weight, ash and crude protein content of 5.38 and 3.93, respectively The percentage was the next highest and the fat content was only 0.31%.

The total dietary fiber accounted for 87.49% of total carbohydrate content, which was 98% as expected. The insoluble dietary fiber was 77.6%, and the water-soluble dietary fiber was 22.4%, and the ratio of insoluble to water-soluble was about 4: 1.

On the other hand, the high ash content of the fiber of the aloe vera oil cell tissue of the present invention is presumably due to the affinity or binding of the fiber mineral. In general, diets rich in cellulose, hemicellulose and fruits or vegetables have been reported to increase mineral content of Ca, Mg, Zn, Cu, etc. in feces (Kay, 1982). Thus, it is well known that dietary fiber reduces the bioavailability of minerals, which is attributed to the binding of monovalent or divalent ions to purified dietary fiber, such as acidic polysaccharides or cellulose, by ion exchange capacity. (Kay, 1982; Harland, 1989).

Example 2

In order to investigate the physical properties of fiber of aloe vera oil cell tissues, the observation of crystal and surface structure, water retention, retention capacity, emulsification capacity and stability, foam formation ability and stability and viscosity characteristics were measured as follows.

(1) crystal and surface structure

The crystal structure was observed by X-ray diffractometer (Bruker model D5005). The operating conditions were copper (Cu K _α ) tube as a large cathode at 40kV / 30mA, and diffraction to 2θ = 10 ~ 60˚ with step size 0.04, the results are shown in Table 2 and shown in FIG.

In addition, the surface structure was observed with a scanning electron microscope (Hitachi SEM model S-4300). That is, the sample was thinly dispersed on a double-sided adhesive tape, vacuum-deposited (100 kPa thick) with Au, and photographed and observed at 1,000 to 20,000 times under conditions of a voltage of 5 kV and a sample inclination of 30 ^o , and the results are shown in FIG. 3.

(2) FT-IR analysis

Fourier transformed infra red (FT-IR) spectra were prepared using a Bio-Rad Model Excaliber (Cambridge, USA) instrument with a resolution of 3 cm ^-1 prepared with KBr discs containing 2 mg of lyophilized UF samples. Was obtained and the result is shown in FIG.

(3) water holding capacity (WHC)

It was determined using the AACC method (1981). That is, 5 g of the sample was weighed into a 50 ml centrifuge tube previously weighed, and a small amount of distilled water was added to each sample, followed by stirring with a glass rod.

After the mixture was completely wet, the mixture was centrifuged at 2000 g for 10 minutes, and then the amount of the supernatant liquid was measured, and the water holding capacity (g of water per g of sample solids) was obtained by the following equation.

WHC = (W ₂ -W _One ) / W _o

Where W _o is the dry sample weight (g), W ₁ is the weight of the tube and the sample (g), W ₂ is the weight of the tube and the sediment (g), and the test is repeated three times for each sample. It is shown in Table 3.

(4) oil absorption capacity (OAC)

Retention capacity was measured by Chakraborty's method (1986). 1 g of sample was weighed and placed in a pre-weighed 50 ml centrifuge tube and mixed thoroughly with 10 ml soybean oil or rice bran oil. The sample-oil mixture was centrifuged at 1600 g for 10 minutes and the supernatant was removed to determine the tube weight, and the fat absorption capacity (g oil per g solid sample) was calculated as follows.

OAC = (W ₂ -W _One ) / W _o

Where W _o is the dry sample weight (g), W ₁ is the weight of the tube and the sample (g), W ₂ is the weight of the tube and the sediment (g), and the test is repeated three times for each sample. It is shown in Table 3.

(5) emulsifying capacity and stability

The emulsification capacity (EC) and emulsification stability (ES) were determined by Yasumatsu et al. , Was repeated three times according to the method (1972). 8 g of each sample was weighed, transferred to a blender, mixed with 100 ml of distilled water and 100 ml of soybean oil, and mixed at high speed for 1 minute to form an emulsion. For each sample, a fixed amount of emulsion (40 ml, Vt) was taken, transferred to a 50 ml centrifuge tube, and centrifuged at 1475 g for 5 minutes. The volume of the emulsion fraction (V _f1 ) was recorded and the tube containing the O / W emulsion fraction was heated in an 80 ° C. water bath for 30 minutes and then cooled to room temperature, which was centrifuged (1475 xg, 5 minutes). The volume of remaining emulsion fraction (V _f2 ) was recorded. EC and ES are respectively represented by the following formulas, and the results are shown in FIG.

EC (%) = (V _f1 / Vt) x 100

ES (%) = (V _f2 / Vt) x 100

(6) foaming capacity and stability

Foam forming ability (FC) and foam stability (FS) were repeated three times using Mitchell's method (1986). 75 ml (Vi) of an aqueous solution of 0 to 3 wt% (containing 1% egg albumin + 0.5% CaCl ₂ ) was mixed for 3 minutes using a high-speed homogenizer. Pour into the measuring cylinder and measure the foam volume (V _f ) immediately. The foam was left to stand at 25 ° C. for 30 minutes and the volume of liquid (Vo) produced under the foam was measured, followed by FC (ml of foam per ml of liquid) and FS (ml of liquid remaining in foam per ml of initial volume). Each was calculated | required by the formula, and the experimental result is shown in FIG. 3 and FIG.

FC = V _f / Vi

                     FS = (Vi-Vo) / Vi

(7) Viscosity Characteristic Measurement

0.1-0.5% of stabilizer (CMC) was mixed with the sample as it was or suspended in distilled water to obtain a 1-3% suspension. Warm to 60 ° C. and cool to 30 ° C. again using a spindle viscometer (Brookfield DV-II +, Brookfield Eng. Labs Inc.). The viscosity was measured by 1 and the results are shown in FIGS. 5 and 6.

Position
(2θ) Height
(cts) Full width at half maximum
(2θ) d-spacing
(A) Relative Intensity
(%) Tip width
(2.θ) 15.06 93.01 0.80 5.88 27.44 0.96 21.64 338.96 2.68 4.11 100.00 3.21 24.82 102.10 0.40 3.59 30.12 0.48 26,27 144.41 0.13 3.39 42.61 0.16

Swelling force
(Ml / g) Conservative
(Water g / solid content g) Ability
(Oil g / solid content g) Fiber of the Invention 27.33 ± 0.76 6.40 ± 0.19 10.32 ± 0.29 Comparative Standard Sample 8.33 ± 0.29 4.43 ± 0.13 4.92 ± 0.08

As a result of the component analysis, the fiber of the aloe vera oil cell tissue of the present invention was found to be a dietary fiber. In general, dietary fiber is divided into polysaccharides and lignin, and polysaccharides are divided into cellulose and non-cellulose polysaccharides (Kay, 1982). The fiber of the aloe vera oil cell tissue of the invention showed the IR absorption characteristics of typical polysaccharides, as shown in Figure 1, by examining the FT-IR characteristics. Therefore, the fiber of the aloe vera oil cell tissue of the present invention is assumed to be polysaccharide rather than lignin, and insoluble dietary fiber is most likely to be cellulose. Thus, to more clearly confirm this, its crystal structure was examined by X-ray diffraction.

In general, there are seven peaks derived from the crystalline component in the range of 2θ = 10 to 40 ^o , of which four are characteristic of natural cellulose (2θ ≒ 15, 17, 22, 34), and around 2θ = 22 ^o The diffraction that appears is known to be due to the precipitation of the surface of cellulose fibers (Wada et al ., 1994).

As shown in Figure 2, the 2θ of the diffraction peaks for the fiber of the aloe vera flow cell tissue of the present invention is 15.0, 21.6, 24.8 and 26.2, the difference between the diffraction pattern of cellulose-I of 2θ = 14.6, 16.6 and 22.6 Indicated. Rather, it was close to the diffraction pattern of cellulose IV such as alpha-cellulose of 2θ = 15.4 and 22.2, but as shown in Table 2, it showed 2θ peak of ammonia cellulose or poly (ethylene glycol) cellulose of cellulose III and was different from alpha-cellulose. Showed.

On the other hand, as can be seen from Figure 3 when the surface structure of the fiber of the aloe vera oil cell tissue of the present invention was observed at 100 and 500 times magnification using a scanning electron microscope showed a fine fibrous structure.

Lee e t al. (2005) said that biocellulose has a fibrous structure, but α-cellulose has a gel-like structure. The surface structure of the fiber of the aloe vera oil cell tissue of the present invention is thought to be similar to that of α-cellulose. In particular, Lee et al. (2005) observed 10,000 times more than biocellulose, which showed a mosaic-like structure with a lot of irregular porosity on the surface, unlike α-cellulose. In the case of the fiber of the aloe vera oil cell tissue of the present invention, This porosity was not observed at 5,000 times magnification. This difference in surface structure is seen in biocellulose. In other words, the morphology of bacterial cellulose is very different based on different culture systems, and it has been reported that in stationary culture, a gelatinous membrane of bacterial cellulose is formed at the air / liquid interface, while a fibrous form is obtained due to high shear force in agitated cultures ( Chao et al ., 2000, 2001).

Meanwhile, Ma Connell et al. According to (1974), the water adsorption capacity is greatly influenced by the type, content and particle size of the dietary fiber. The composition, particle size, pH and ionic strength of dietary fiber are reported to be the main factors (Eastwood, 1986). In the case of cellulose, if the crystallinity is high, the water bond is reduced by direct hydrogen bonding.

Typically, the water adsorption capacity of dietary fiber sources is well known to be related to the mechanism of lowering digestion, increasing stool volume and weight, and lowering serum triglyceride (Schneeman, 1987).

Therefore, the water retention of dietary fiber can be seen to have an important meaning as an indicator of physiological function, and the water retention was measured while comparing the water retention of the fiber of the aloe vera oil cell tissue of the present invention with alpha-cellulose.

As can be seen from Table 3, the retention capacity of the fiber and alpha-cellulose of the aloe vera oil cell tissue of the present invention was 6.40 ± 0.19 and 4.43 ± 0.13 (water g / g solids), 1.4 times higher than commercial alpha-cellulose fiber. Appeared to be better. This result is a relatively high value of the water holding capacity, Ang (1991) reported that the smaller the particle size of the cellulose increases the water retention, maintaining 4 to 10 times depending on the fiber length, the water holding capacity of alpha cellulose is relatively well It was a consistent range of values, similar to a commercial pectin. On the other hand, since the fiber of the aloe vera oil cell tissue of the present invention absorbs about 6.4 times of water, considering that the water adsorption power of conventional dietary fiber is about 4 times (Cadden, 1987), it has a characteristic of absorbing relatively high water. It was confirmed.

In addition, the retention capacity of the fiber and alpha-cellulose of the aloe vera oil cell tissue of the present invention is 10.32 ± 0.29 and 4.92 ± 0.08 (g oil retained / g solid), respectively, the retention capacity was relatively higher than the water retention capacity. The fiber of the aloe vera flow cell tissue of the invention showed about 2 times higher value than commercial alpha-cellulose.

In general, the holding capacity is relatively low compared to the water holding capacity, and the range is known to be 30 to 80% (Lee et al. , 2005) . In the case of powdered cellulose, Ang (1991) has a holding capacity of 2.5 to 8.5 times. Therefore, it was confirmed that the fiber of the aloe vera oil cell tissue of the present invention has high retention, and the retention capacity is greater than the water retention capacity.

On the other hand, swelling power (SW) is an important property of the hydration-related properties such as water retention and retention capacity in aloe dried products, the higher the value is known to be less physical and chemical changes during dehydration and less dry damage (Femenia et al. ., 2003;. Simal et al , 2000).

The swelling power of the aloe vera oil cell tissue fiber of the present invention was 27.33 ± 0.76 mL / g, which was about 3.3 times higher than the alpha-cellulose (8.33 ± 0.29 mL / g).

In general, in the case of starch, the swelling force is limited to have a strong micelle structure in the starch particles, which means that the binding force is weak (Elliason, 1980). Therefore, the aloe vera oil cell tissue fiber of the present invention was considered to have a weaker binding force than alpha-cellulose, and thus the water absorption capacity (water retention capacity) is higher, so the aloe vera oil cell tissue fiber of the present invention is more than the alpha-cellulose. The results are in good agreement with the results of high water retention.

O / W emulsions are also suspensions in which fat droplets are suspended in water stabilized by a surfactant or emulsifier at the oil / water interface. For proteins, the ability to act as an emulsifier depends on the amphoteric properties, with effects on solubility, degree of surface denaturation, fat / protein ratio, emulsion viscosity.

As a result of examining the emulsification capacity and emulsion stability of the addition of 0.5% xanthan gum which is widely used as an emulsion stabilizer is shown in Figure 4, the emulsification capacity of the aloe vera oil cell tissue fiber of the present invention is about 65% It was lower than xanthan gum (about 90%) but about twice as high as alpha-cellulose. In the control group without xanthan gum, water and oil were separated within a few minutes immediately after emulsification, while the 0.5% xanthan gum addition group showed no emulsification at all until 24 hours and showed high emulsification stability. Xanthan gum's high emulsification stability has been found to be due to high yield stress and cohesiveness under static conditions. In general, high molecular weight materials such as polysaccharides are dissolved in water and oil dispersion medium to increase the viscosity to prevent creaming or increase viscosity and elasticity. It has been reported that this strong adsorption layer enhances emulsion stability by preventing coalescence between oil particles (Hennock et al ., 1984).

In addition, the foam is incorporated into the aqueous solution by physical stirring and aeration, contributing to the smoothness, lightness, flavor, dispersion and flavor of the food. It is known to enhance the formation of smaller air cells in the tissue and mouth and to organize the water phase around fat globules. In particular, the foaming of protein solutions is desirable in many food applications, with high foaming capacity and foam stability being required in cakes, breads, whipped topping, ice creams and desserts (Dickinson, 1989).

In general, the primary function of proteins in the foam is to reduce the surface tension at the air / liquid interface to facilitate the incorporation of air into the liquid phase and to stabilize the foam by forming agglomerated films around the droplets. The foaming ability and foam stability of cellular fibers and alpha-cellulose were investigated.

As shown in Figure 5 and Figure 6, the aloe vera oil cell tissue fiber of the present invention did not show the foaming ability like alpha-cellulose at a concentration of 2% (w / v), but the foaming ability is increased as the concentration is lowered It showed foaming ability. In particular, the foam stability was improved about three times than the control, and about two times higher than the alpha-cellulose.

In general, foaming is mainly related to rapid intermolecular adsorption and rearrangement, while stability of foam stabilization against gravity and mechanical stress is known to be imparted by the formation of a flexible cohesive film at the interface (Dickinson, 1989). .

On the other hand, the dietary fiber having viscosity and gel formation ability increases the viscosity of the digestive tract contents and affects the speed and absorption of the digestive tract of the contents. (Ebihara and Kiriyama, 1990).

Therefore, the viscosity of the aloe vera oil cell tissue fibers and alpha-cellulose of the present invention was measured using a rotational viscometer at 3 to 100 rpm, and as shown in FIG. Since cellulose itself does not show the ability to disperse in water, the flow behavior of the 1.5% (w / v) dispersion in the stabilizer (0.3% CMC) solution was investigated, but the present invention contained 0.5 to 1.5% (w / v) CMC. The viscosity of aloe vera oil cell tissue fiber and alpha-cellulose of the aloe vera oil showed a sharp decrease with increasing the rotational speed (rpm) and showed pseudoplastic properties.

As the concentration increased, the aloe vera oil cell tissue fibers of the present invention showed a higher viscosity at the corresponding shear rate. As shown in FIG. 8, the viscosity value at a rotational speed of 60 rpm was 0.5-1.5% (w / v). Viscosity of aloe vera oil cell tissue fiber of) was similar to that of alpha-cellulose at 0.5%, whereas the concentration-increased viscosity increased, unlike alpha-cellulose. In particular, aloe vera oil cell tissue fiber showed a significant increase in viscosity with concentration, showing a viscosity increase of about 1.8 times higher than 0.5% at 1.5% concentration and good dispersibility.

Example 3

To investigate the physiological functional characteristics of aloe vera oil cell tissue fiber, delayed absorption of glucose and bile acid was measured.

(1) Delayed Effect of Glucose Absorption by In vitro Method

The effect of delaying glucose absorption was measured by analyzing the glucose concentration of the dialysis external fluid because glucose in the free state passes through the dialysis membrane as it is, but glucose adsorbed on the polymer material does not pass through the dialysis membrane (Adiotomre et al. , 1990). A dialysis membrane (Sigma D7884: MW cut-off <1200) having a width of 3.2 cm ² and a length of 10 cm was used after soaking in 0.1% sodium azide solution overnight. After one end of the dialysis membrane was tightly bound with a cotton thread, 0.2 g of the sample was placed in the dialysis membrane, and 6 ml of 0.1% sodium azide solution in which 36 mg of glucose was dissolved therein. The dialysis membrane on the other side was also tightly bound, placed in a 150 ml container, and hydrated for 14 hours. The control was performed in the same manner except for the sample. After completion of the hydration, 100 ml of 0.1% sodium azide solution was added to the vessel, and a permeation experiment was performed for 24 hours at 100 rpm in a shaking thermostat maintained at 37 ° C. Glucose content was measured by taking 1 ml of the dialysis external solution at regular intervals (30 minutes to 24 hours), and the delayed absorption effect was calculated by the following equation. The results are shown in Table 7, and the change in glucose permeability over time was plotted. 9 is shown.

% Glucose Absorption Delay Rate = 100-(Total Glucose Permeated from Fiber-Containing Dialysis Membrane / Total Glucose Permeated from Fiber-Free Dialysis Membrane × 100)

At this time, the glucose content was measured according to DNS or ABTS method (White and Kennedy, 1981). In the ABTS method, ABTS reagent (60 mg of glucose oxidase, 6 mg of peroxidase, and 50 mg of ABTS were buffered in 0.12 M phosphate in a sample dissolved in 0.1% sodium azide solution and 1 ml of standard glucose solution. After 5 ml of the solution (dissolved in 250 ml of phosphate buffer) was added, the mixture was left at room temperature for 30 to 40 minutes and absorbance at 450 nm was measured.

(2) Effect of delaying bile acid absorption by in vitro method

The effect of delaying bile acid absorption is similar to that of glucose, according to Adiotomre et al. , According to the method (1990). Since free bile acid leaves the dialysis membrane, 15 mmol of taurocholic acid (taurocholic acid: Sigma T-) per 1 liter in 0.05 M phosphate buffer solution (pH 7.0) prepared by adding 0.2 g of sample inside the dialysis membrane and prepared with 0.1% sodium azide solution. 6 ml of the solution dissolved in 4009) was added and the end of the dialysis membrane was tightly bound. It was hydrated for 14 hours in a 150 ml container, and the control was carried out in the same manner except for the sample. 100 ml of 0.05 M phosphate buffer (pH 7.0) prepared with 0.1% sodium azide solution was added thereto, followed by shaking at 100 rpm in a constant temperature water bath at 37 ° C. for 72 hours. The bile acid content was measured by taking 1 ml at regular time intervals, and the results are shown in FIG. 10. The bile acid absorption retardation effect was calculated by the following equation, and the results are shown in Table 8.

Delay rate of bile acid absorption (%) = 100-(total amount of bile acid permeated from the dialysis membrane containing fiber / total amount of bile acid permeated from the dialysis membrane without fiber × 100)

At this time, the bile acid content is Boyd et al. , According to the method (1966). That is, 5 ml of 70% H ₂ SO ₄ solution was added to 1 ml of bile acid (Sigma B-8756) solution, and 5 minutes later, 1 ml of 0.25% furfural solution was added. After leaving for 60 minutes, the absorbance was measured at 510 nm, the maximum absorption wavelength after the maximum color development of pink.

(3) Effect of improving obesity and constipation by animal experiment

To investigate the effects of aloe vera oil cell tissue fiber on the improvement of obesity and constipation, the following animal experiments were conducted in rats.

Experimental animals: Male rats (Sprague Dawley), 6 weeks old, were purchased from Hyochang Science and used after 1 week of adaptation.

Formulation of the experimental diet: The diet used in the animal experiment was an open source diet (D12492, Research diet, Inc. New Bruswick, USA), a high-fat diet for obesity induction and constipation induction. Table 4 is as follows. At this time, the experimental diet used aloe vera oil cell tissue fiber, the addition level was 5%.

Breeding conditions and test groups: Experimental animals have a temperature of 20.7 to 22.1 ℃, relative humidity of 49.9 to 54.2%, 10 to 15 times of ventilation, and 12 hours (8 am to 8 pm). It was raised in the environment animal breeding room. After one week of adaptation, three to four animals were isolated from the wire cage and divided into a general feed group, a high fat feed group, and a high fat feed + dietary fiber (5%) group. . At this time, each group was a group of 10 horses with similar weight.

Meanwhile, distilled water was used as drinking water, and drinking water and diet were freely consumed. Dietary intake was measured 2-3 times a week and converted to daily intake, and body weight was measured immediately before the start of the experimental diet and 30 days after administration, and the results are shown in Table 9.

Sampling : After 30 days of experimental diets, animals were fasted for 12 hours and anesthetized with ethyl ether, and blood was collected from the abdominal aorta with about 5-7 ml of blood. The whole blood was passed through a micro hematocrit tube (GRAF Cat. No. 5.530-06) containing an anticoagulant (heparin: Sigma H7005), followed by centrifugation at 3000 rpm (4 ° C) for 10 minutes. It was isolated and used as a sample of plasma analysis.

On the other hand, after dissection, the organs such as liver, spleen, kidney, testicles were cut out, and after extraction, treated with saline solution, water was removed with filter paper (Whatman No. 2), and then weighed.

In addition, adipose tissue was excised and weighed visceral fat (Inguinal fat pads), colon and liver tissue was randomly cut about 0.5 ~ 1cm and fixed in 10% formalin solution to confirm histological findings, RNA extracts were stored frozen in liquid nitrogen until analysis.

Histochemical analysis : Colon and liver tissues were excised, fixed in 10% formalin solution (dissolved in phosphate buffer pH 7.4) and washed with water, followed by paraffin embedding. Then, using a microtome (microtome) to make a tissue section to a thickness of 5 ~ 7㎛ and then stained. After dyeing, moisture was removed and embedding was carried out using canadia acid.

After deparaffinization, hematoxilin was stained for about 10 minutes, washed with water, and stained with eosin for 5 minutes. Hematoxilin-Eosin (HE) staining was then observed under an optical microscope.

Meanwhile, an immunohistochemistry experiment was performed to investigate protein expression related to colon disease. That is, after deparaffinization, peroxidase blocking was performed using PBST, the first antibody was treated, and after blocking, the second antibody was treated. Was carried out. The antibody used in this case is shown in Table 5.

RT-PCR : To measure constipation-related transcription expression factors, the large intestine was pulverized with liquid nitrogen, and RNA was isolated using TRIZOL reagent (Invitrogen, USA), followed by RT according to the method of Tong et al. (2005). (reverse transcription) reaction and PCR were performed.

Detection transcriptional expression factors and primers used for the same are shown in Table 6.

Statistical processing : All experimental results for this experiment were expressed as mean and standard deviation, and statistical significance was tested by Sigma Stat 3.1 using One Way ANOVA.

(g / 1kg diet) ingredient Diet ND HFD HFD + ADF Casein (80 mesh) (Casein, 80mesh) 200 200 200 L-Cystine 3 3 3 Corn Starch 245 0 0 Maltodextrin 10 125 125 125 Sucrose 68.8 68.8 18.8 Cellulose, BW200 50 50 50 Soybean Oil 25 25 25 Lard - 245 245 Mineral Mix, S10026 10 10 10 DiCalcium Phosphate 13 13 13 Calcium Carbonate 5.5 5.5 5.5 Potassium Citrate, 1H ₂ O 16.5 16.5 16.5 Vitamin Mix, V10001 10 10 10 Choline Bitartrate 2 2 2 FD & C Blue Dye # 1 0.05 0.05 0.05 Aloe vera milk cell tissue fiber
(Aloe Dietary Fiber) - - 50 Total 773.85 773.85 773.85

ND: Normal diet, HFD: High fat diet, ADF: High fat diet + Aloe vera milk cell tissue fiber.

Antibody Source Binding site Anti-smooth muscle myosin heavy chain (SMMHC) Sigma M-7786 Myosin heavy chain polypeptides of 204 and 200kDa Anti-smoothelin (R4A) (SM) Santacruz
smoothelin: SC-28562 Polypeptide of c. 60 kDa (cytoskeleton-associated protein)

Primer (F / R) Size (bp) Rat Prox1 5'-AAG CCA GCG GAC TCT CTA GC-3 '
5'-ACA GGT CGC CAT CTT CAT CA-3 ' 518 Rat c-kit 5'-ATCTGCTCTGCGTCCTGTTG-3 '
5'-GTTGGGGACGAACTTCAGGT-3 ' 491 Rat GAPDH 5'-TGT CAG CAA TGC ATC CTG CAC CAC C-3 '
5'-GAA GTC ACA GGA GAC AAC CTG GTC C-3 ' 420

30 minutes after dialysis 1 hour after dialysis 2 hours after dialysis Dialysate
Glucose
(%, w / v) GDRI (%) ² Dialysate
Glucose
(%, w / v) GDRI (%) ² Dialysate
Glucose
(%, w / v) GDRI (%) ² Control group 1.26 ± 0.11 ¹⁾ 0 1.35 ± 0.02 0 1.48 ± 0.13 0 Aloe vera milk cell tissue fiber 0.80 ± 0.07 35.24 ± 0.09 1.01 ± 0.06 25.73 ± 0.06 1.18 ± 0.08 20.32 ± 0.02 Alpha-cellulose 1.06 ± 0.04 13.69 ± 0.04 1.18 ± 0.02 12.82 ± 0.02 1.36 ± 0.12 8.24 ± 0.09

1) mean ± standard deviation

2) glucose absorption delay rate

1 hour after dialysis 2 hours after dialysis Bile acid in dialysate (%, w / v) BDRI (%) ² Bile acid in dialysate (%, w / v) BDRI (%) ² Control group 0.33 ± 0.03 ¹ 0 0.53 ± 0.07 0 Aloe vera milk cell tissue fiber 0.15 + 0.03 53.13 ± 0.2 0.38 ± 0.03 28.30 ± 0.08 Alpha-cellulose 0.30 ± 0.03 9.09 ± 0.1 0.40 ± 0.04 24.53 ± 0.10

1) mean ± standard deviation

2) delay of bile acid absorption

Test group ¹ Initial weight (g) Final weight (g) Weight gain (g / day) Dietary Intake
(g / day) Dietary Efficiency (%) ² ND 266.70 ±
10.89 ³ 403.10 ±
32.43 4.55 ±
0.96 ^b4 22.76 ±
2.22 ^a 20.26 ±
2.03 ^b HFD 266.80 ±
8.49 455.43 ±
21.10 6.29 ±
1.06 ^a 18.72 ±
2.16 ^b 34.35 ±
0.09 ^a HFD + ADF 266.60 ±
8.86 456.61 ±
10.21 6.33 ±
0.60 ^a 19.42 ±
1.57 ^b 32.75 ±
0.043 ^a

1) ND: Normal diet, HFD: High fat diet, ADF: High fat diet + Aloe vera oil cell tissue fiber (Aloe dietary fiber 5%, w / w).

2) Dietary efficiency (%) = (weight gain / food intake) × 100

3) The value is mean ± standard deviation (n = 10).

4) The values with different subscripts in the same column are significantly different as p <0.01 in ANOVA test by Duncan's multiple range test.

As can be seen from FIG. 9, the glucose transmission rate of the control group increased rapidly to the initial 6 hours of dialysis and reached about 85%, and then increased slightly to 24% after 24 hours. On the other hand, aloe vera oil cell tissue fiber addition group reached about 75% after 6 hours, and gradually increased to about 80% after 24 hours.

therefore. It could be confirmed that Aloe vera oil cell tissue fiber showed a delayed effect of glucose, which is probably due to the entrapping effect that traps glucose in its structure (Schneeman, 1987). ).

Glucose absorption delay rate at the beginning of dialysis was used as an indicator for determining the delayed effect of glucose absorption. Therefore, the glucose absorption inhibition index was calculated at 30 minutes, 1 hour, and 2 hours. As can be seen from Table 7, aloe vera oil cells Tissue fiber showed a 20.32 to 35.20% delay in absorption in the 2-hour in vitro glucose uptake experiment using dialysis membrane, which was about 2.5 times higher than the comparative standard alpha-cellulose.

Glucose absorption inhibition index is about 30 minutes after dialysis of the water-soluble dietary fiber alginic acid, guar gum, carboxymethyl cellulose (CM-cellulose) and citrus pectin among commercial fiber. As a% level, aloe vera oil cell tissue fiber has a very high delay in glucose absorption above these values, and antidiabetic effect by inhibition of glucose absorption is expected.

In the meantime, there have been animal and clinical studies of diabetes in aloe gel or prostate extract. Okyar e t al. (2001) used extracts of leaves and gels of aloe vera using ND (non-diabetic), type I (IDDM; insulin dependent diabetes millitus) and type II (NIDDM; (non-insulin dependent diabetes millitus) mice. The results showed that the rats had no effect of lowering blood glucose levels, but the extracts of leaves and gels of Aloe vera showed hypoglycemic activity in the IDDM and NIDDM strains, and the hypoglycemic agent, glibenclamide. In contrast, the gel extract showed lowering blood glucose only in the NIDDM strain, and the gel-free pulp of Aloe Vera leaves was useful for the treatment of NIDDM.

Rajendran e t al. (2007) also reported that diabetic rats treated with A. vera sap significantly increased glycogen in body weight and liver, and significantly decreased blood glucose, urine glucose levels and blood lipids compared to other groups.

On the other hand, in order to determine the effect of delaying the bile acid absorption by the in vitro method of aloe vera oil cell tissue fiber as measured from the measurement of the amount of bile acid dialyzed through the dialysis membrane over time for 72 hours as shown in Figure 10 The bile acid permeation rate of the control group increased relatively rapidly until the first 3 hours and then gradually increased to 72 hours, and nearly 90% after 72 hours. This trend was similar to glucose permeation.

Therefore, it was found that the sample addition delays the permeation of bile acids at the corresponding time. To further clarify the delayed absorption of bile acids, the bile acid retardation index between 1 and 2 hours at the beginning of dialysis ,%), The delayed absorption effect of in vitro bile acid for 2 hours using dialysis membrane was very high (53.13 ~ 28.30%). The delayed effect of bile acid absorption was at least 1.2 to 6 times higher than that of the comparative standard alpha = cellulose.

Considering that the bile acid absorption inhibition index of the water-soluble dietary fiber in the reported commercial fiber is 30.4% of citrus fruits, guar gum 22.3%, 17.0% of alginic acid and 13.4% of apple pectin (Lee and Lee, 1996), the aloe vera oil of the present invention Cellular fiber value is significantly higher, it can be seen that the bile acid absorption delay effect is excellent.

Story (1985) suggests that viscous dietary fiber delays the absorption of glucose in the intestine, thereby reducing the insulin level in the blood, as well as reducing the free bile acid content by binding to bile acids, thereby changing the content of bile acids that are reabsorbed. Dixit and Joshi (1983) reported that aloe vera leaf extracts reduced LDL and VLDL-cholesterol by 79% and 37.5%, respectively, and decreased HDL in animal experiments. It has been reported that the total cholesterol ratio is significantly increased compared to the initial level.

Therefore, it was determined that the delayed absorption of glucose and bile acids of Aloe vera milk cell tissue fiber could be expressed as an effect of inhibiting diabetes and atherosclerosis.

In addition, as can be seen from Table 9, the average weight of the rats before the experiment ranged from 265 to 266.7 g with similar values without any difference between the groups. However, after 30 days of breeding, the mean weight was 403.10 ± 32.43g in the normal diet group and 455.43 ± 21.10g in the high-fat diet group, which showed a higher weight gain in the high-fat diet group.

The weight gain was 6.29 ± 1.06 g / day in the high-fat diet group and 4.55 ± 0.96 g / day in the general diet group. The weight gain rate of the high-fat diet group was about 23.6% higher than that of the normal diet group. Four weeks of high-fat diet significantly increased body weight and obesity was induced. On the other hand, the average weight gain rate of the Aloe vera oil-tissue fiber-added group was 6.33 ± 0.60g / day, which was the same as that of the high-fat diet group, which did not show the weight loss effect, which was statistically significant (p < 0.01). However, due to the high calorie of the high-fat diet, the dietary intake was higher in the high-fat diet group than in the general diet, but the dietary efficiency was higher. The dietary efficiency of Aloe vera oil cell tissue fiber supplemented group was about 32.75%, which is higher than that of general diet group (20.26 ± 2.03%) but slightly lower than that of high-fat diet group (34.35 ± 0.09%). The addition of cellular fiber is believed to have the effect of inhibiting some weight gain.

In addition, as can be seen from Figure 11, high fat diet slightly increased the weight of the liver, spleen, kidney and testicles than in the general diet group. On the other hand, the organ weight of the aloe vera oil-tissue fibrous supplemented group was similar to that of the normal diet group. The organs with the largest weight gain by the high-fat diet were relatively hepatic. The liver weight was 11.6 ± 0.92 g in the high-fat diet, which was about 17% higher than the 9.9 ± 1.31 g in the normal diet, but aloe vera oil cells Tissue fiber added group showed an average diet level of 10.3 g.

Although not shown in the data, the results of the analysis of aspartate aminotransferase (AST) and alanine transaminase (ALT) were similarly increased in the high-fat diet group compared to the general diet group and decreased again by the addition of aloe vera oil cell tissue fiber. . However, the error range was relatively large and there was no statistical significance.

This increase in liver weight was thought to be related to the accumulation of fat in liver tissue, and the fat deposition in liver tissue was examined. As can be seen from FIG. 12 showing the results, the distribution of fat granules was observed in the high-fat diet group. However, in the aloe vera oil cell tissue fiber addition group, the presence of fat globules could not be observed in the aloe vera oil cell tissue fiber addition group, as in the general diet group. It is presumed that the fatty liver can be reduced by greatly relieving the degree, which is because aloe vera oil cell tissue fiber inhibits fat absorption by inhibiting fat absorption.

On the other hand, as shown in Figure 13, the result of measuring the weight of the inguinal fat (Inguinal fat pads) with adipose tissue, the weight of the visceral fat of the general diet group was 5.2 ± 1.12g, but in the group fed high-fat diet 10.2 ± The weight of visceral fat was about twice that of 1.82g. However, in the high fat diet group, aloe vera oil cell fiber was reduced by about 10%, and showed a statistical significance (p <0.05), indicating that the aloe vera cell tissue fiber inhibited the accumulation of visceral fat. Appeared to be.

After fasting 24 hours before dissection of the animals, the general diet was ingested as shown in FIG. 14, which shows the result of measuring the number of residual stools to determine how much the stool movement in the colon was made on the last day of the experiment. In the case of about 2.5 stools found in the large intestine, 4.2 stools were observed in the diet of high fat, and the stools shifted about 1.7 times slower in the high-fat diet group. On the other hand, in the group of aloe vera oil cell fiber intake was 3.6, dietary fiber intake was thought to speed up the movement of the stool slightly. Although it was not documented due to lack of statistical significance, the intestinal transit time obtained by measuring the time of mutant blue after mixing with 1 g of 10% comassie brilliant blue dye after 12 hours of fasting showed similar trends. The tissue fiber added group had a time difference of about 2-3 times that of the high fat diet group.

Constipation-related transcriptional expression factors were investigated because the number of bowel stools and the intestinal transit time could infer the effect of improving the bowel activity of aloe vera oil cell tissue fiber.

Recently, the c-kit gene has been known to reduce expression in constipation patients, and the Prox 1 gene has been known to be expressed when colon cancer is induced (Tong et al ., 2005; Shimoda et al ., 2006; Petrova et al. ., 2008;).

As can be seen from FIG. 15, the expressions of c-kit and Prox 1 genes of the general diet group, the high fat diet group, and the aloe vera oil cell tissue fiber addition group were compared with GAPDH (glyceraldehydes-3-phosphate dehydrogenase). As a result of confirming in three colons of each group, it was confirmed that the individual differences were severe. However, the general diet group and the aloe vera oil cell fiber-added group showed different expression levels of the high-fat diet group and the c-kit gene.

In addition, the prox-1 gene induced by colon cancer was increased in the high fat diet group, but not in the aloe vera cell tissue fiber group.

On the other hand, myosin heavy chain (SMMHC) and smoothelin (SM) of smooth muscle in colorectal moltility dsiorders are known as markers related to the abnormality of colonic epithelium (Wedel et al ., 2006). Wedel et al. (2006) report that the immunoreactivity of these proteins is 70% in slow-transit constipation.

Therefore, the expression of these proteins was examined by immunohistochemistry. As can be seen from FIG. 16, in the case of Myosin, the counting of positive cells of myosin in the colonic epithelium was not significant. The results showed that there was a general decrease in colon epithelium in the high-fat diet group and a slight increase in the aloe vera oil cell tissue fiber intake group.

On the other hand, smoothelin showed a tendency to decrease in the colon epithelium of the high-fat diet group than the general diet group. On the other hand, aloe vera oil cell tissue fiber addition group showed a tendency to increase although there is a slight difference in expression. The expression level was general diet group> aloe vera oil cell tissue fiber addition group> high fat diet group.

Based on the above results, it can be inferred that dietary obesity-induced rats fed with high-fat diet and aloe vera oil cell fibrous fiber had some effects on improving obesity and constipation. there was.

Normally, in these animal experiments, the level of dietary fiber addition is 5-20%, and the effect is increased depending on the concentration. In this experiment, the amount of aloe vera oil cell tissue fiber added was 5%, which is the minimum level.

1 is a graph showing the FT-IR spectrum of aloe vera oil cell tissue fiber according to the present invention,

2 is a graph showing the results of X-ray diffraction analysis of aloe vera oil cell tissue fiber according to the present invention,

Figure 3 is a scanning micrograph of the aloe vera oil cell tissue fiber according to the present invention,

Figure 4 is a graph showing the emulsification capacity and emulsion stability of the aloe vera oil cell tissue fiber according to the present invention,

5 is a graph showing the foaming ability of the aloe vera oil cell tissue fiber according to the present invention,

6 is a graph showing the foam stability of the aloe vera oil cell tissue fiber according to the present invention,

7 is a graph showing the viscosity of aloe vera oil cell tissue fibers and alpha-cellulose according to the present invention measured by a rotational viscometer, the left is alpha-cellulose, the right is the viscosity of the aloe vera oil cell tissue fibers according to the present invention ,

8 is a graph showing the viscosity of aloe vera oil cell tissue fiber and alpha-cellulose according to the present invention at a rotational speed of 60rpm,

9 is a graph showing changes in glucose permeability over time of aloe vera oil cell tissue fibers according to the present invention,

10 is a graph showing the change over time of bile acid permeability of aloe vera oil cell tissue fiber according to the present invention,

11 is a graph showing the results of measuring the weight of the organs of rats fed aloe vera oil cell tissue fiber according to the present invention,

12 is a photograph for confirming the fate in the liver tissue of rats ingested aloe vera oil cell tissue fiber according to the present invention,

Figure 13 is a graph showing the results of measuring the weight of fat gut in rats ingested aloe vera oil cell tissue fiber according to the present invention,

14 is a graph showing the results of measuring the number of residual feces in the large intestine of rats fed aloe vera oil cell tissue fiber according to the present invention,

15 is a photograph showing the expression of c-kit and Prox 1 gene in rats fed aloe vera oil cell tissue fiber according to the present invention.

Figure 16 is a colon biopsy photograph of rats ingested aloe vera oil cell tissue fiber according to the present invention.

Claims (5)

After washing with aloe vera, it is peeled to remove alloin and obtained a gel of flow cell tissue, followed by centrifugation to separate and fractionate the fiber and gel of aloe vera, take the aloe fiber fraction and freeze-dry it, and then powder it. Fiber manufacturing method of aloe vera oil cell tissue for dietary fiber. A dietary fiber composition for delaying glucose absorption comprising as an active ingredient the fiber of aloe vera oil cell tissue prepared by the method of claim 1. A dietary fiber composition for delaying bile acid absorption comprising aloe vera oil cell tissue prepared by the method of claim 1 as an active ingredient. Dietary fiber composition for improving obesity comprising the fiber of aloe vera oil cell tissue prepared by the method of claim 1 as an active ingredient. Dietary fiber composition for improving constipation comprising the fiber of the aloe vera oil cell tissue prepared by the method of claim 1 as an active ingredient.

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