KR100970455B1 - Method for preparing extract of ginseng using ultra high pressure - Google Patents

Abstract

Ginsenosides are extracted from ginseng under a pressure of 20 to 110 MPa, and beta-glucanase, pectinase, hemicellulase and cellulase before or simultaneously with extraction. Disclosed is an extraction method capable of maximizing ginsenoside extraction efficiency by hydrolyzing ginseng by adding one or more enzymes selected from the group consisting of arabinase and xylanase.

Ultra High Pressure, Ginsenoside, Ginseng, Red Ginseng, Enzyme, Hydrolysis, Pectinase, Rg2, Rg3, Rb1, Cellulose

Description

Method for preparing ginseng extract using ultra high pressure {Method for preparing extract of ginseng using ultra high pressure}

The present invention relates to a method for preparing a ginseng extract. More specifically, the present invention relates to a method for preparing a ginseng extract with a high content of ginsenosides. The present invention relates to a method for preparing a ginseng extract having a high content of Rg1, Rg2, Rg3 and Rb1 among ginsenosides.

Ginseng is the broadest concept that includes ginseng, white ginseng, red ginseng, wild ginseng, camphor, rice ginseng, and original ginseng. Ginseng is a natural product that has been used as a very valuable medicine in oriental medicine, such as China. Many documents contain the effects of ginseng. However, despite the long history of use, scientific identification through clinical research is insufficient. Nevertheless, even though modern medicine has been introduced, the popularity of ginseng as a medicine is not falling, and it is the first place among the health functional foods. .

The main functional ingredient of ginseng is ginseng saponin called "ginsenoside" which is specially named only ginseng saponin among various saponins of plants.

Scientific research on ginseng components began after the 1960s. The chemical structure of saponins is a glycoside that consists of sugar and aglycone. When these saponins are absorbed by the body, they are broken down by the unique microorganisms in the human intestine and absorbed into the body. However, the intestinal microorganisms that break down ginseng saponins differ depending on the human constitution and dietary habits, and their presence and degree of retention. This can cause differences in efficacy after taking ginseng. Some people only absorb the diol form of saponins, some people only absorb the triol form, and some people do not absorb ginseng saponins at all.

The difference between white ginseng and red ginseng is the difference in the content and content of ginsenosides contained. White ginseng mainly contains ginsenosides Ra, Rb1, Rb2, Rc, Rg1, Re and the like. The red ginseng forms a physicochemical converter in the process of steaming the components in the white ginseng. For example, a large amount of ginsenoside Rg3 is produced in the steaming process. In addition, red ginseng contains small amounts of ginsenosides Rh2 and Rh1. These ingredients have different biological activities. White ginseng contains high molecular weight polysaccharides, but acidic polysaccharides are lower than red ginseng. Red ginseng contains a large amount of polysaccharide whose molecular weight is smaller than that of white ginseng.

The main components of ginseng are ginsenosides, which are saponins, and ginsenosides Rb1, Rb2, and Rc, which are protopanaxadiols, and ginsenosides Re, Rg1, and Rf, which are protopanaxatriols, are known. Representative pharmacological actions of these components include anti-cancer activity, anti-inflammatory, anti-diabetic action. With these components, using cancer cells directly to measure anti-cancer activity in vitro ( in vitro ), there is no activity. However, when these components are administered orally, they are metabolized by enterobacteriaceae and converted to compounds such as compound K, which shows strong cancer cell toxicity and cancer metastasis inhibitory activity.

When ginsenosides are metabolized by the intestinal bacteria described above, they are as follows. First, ginsenosides Rb1, Rb2, and Rc, which are protopanaxadiol-based compounds, are metabolized into compound K via ginsenoside F2. This metabolic reaction is catalyzed by strains of the genus Bacteroides , Fusobacterium genus, Provetella genus, and the like. In addition, ginsenosides Re, Rg1, Rf, etc., which are protopanaxatriol-based compounds, are metabolized to ginsenosides Rh1 or F1 by the strains, and further metabolized to protopanaxatriol.

The components of ginseng can also be converted by physicochemical methods. For example, ginsenosides Rb1, Rb2, Rc, etc. in ginseng may be converted to ginsenoside Rg3 by heat treatment. Taking ginseng containing the converted ginseng saponin can be converted to ginsenoside Rh2 by intestinal bacteria in the intestine and further converted to protopanxadiol.

The difference between the ingredients is closely related to the difference in ginseng drug efficacy. If ginsenosides Rb1, Rb2, Rc containing a lot of white ginseng compound K can be transferred to the blood. On the other hand, in the case of red ginseng with a lot of ginsenoside Rg3, ginsenoside Rh2 can be transferred to the blood. In addition to other ginseng saponins may also be different in the transition to the blood, so in evaluating the efficacy of ginseng should be considered as above.

Extraction is the most important process for separating useful components from plants. Many extraction methods have been used to extract ginsenoside, a useful ingredient from ginseng. The conventional extraction method is a heating extraction method using a solvent. This extraction method takes a long time and has the disadvantage of low extraction efficiency. In addition, many of the components are heat instable and thus have a problem of being destroyed during the heat extraction process.

One of the new technologies introduced to compensate for these drawbacks is the extraction method using ultrasonic waves. Ultrasonic extraction can deliver energy quickly to the solvent as well as directly to the sample to be extracted. That is, the ultrasonic technology can increase the efficiency of the extraction solvent, the cell membrane is destroyed by the mechanical shock of the sonic vibration to facilitate the elution of the cell contents. However, this method has a problem that is difficult to apply industrially.

On the other hand, supercritical extraction method using carbon dioxide is also used. This method is easy to extract fat-soluble substances but has limitations in extracting water-soluble components. These new technologies have problems that require a long time to extract or consume a lot of energy.

An object of one embodiment of the present invention is to provide a ginseng extract.

Another object of an embodiment of the present invention is to provide a ginseng extract with a high ginsenoside content.

It is an object of another embodiment of the present invention to provide a ginseng extract having a high content of Rg1, in particular among ginsenosides.

It is an object of another embodiment of the present invention to provide a ginseng extract having a high content of Rg2, in particular among ginsenosides.

It is an object of another embodiment of the present invention to provide a ginseng extract having a high content of Rg3, in particular among ginsenosides.

It is an object of another embodiment of the present invention to provide a ginseng extract having a high content of Rb1 among ginsenosides.

Method for preparing ginseng extract using ultra-high pressure according to an embodiment of the present invention is characterized in that it comprises extracting ginsenoside from ginseng under a pressure of 20 to 110MPa.

In the method of preparing ginseng extract using ultra high pressure according to another embodiment of the present invention, beta-glucanase (p-glucanase), pectinase, hemicellulase (hemicellulase) before or simultaneously with the extraction And hydrolyzing ginseng by adding one or more enzymes selected from the group consisting of cellulase, arabinase and xylanase.

Health food composition according to an embodiment of the present invention is characterized in that it comprises a ginseng extract prepared by a method comprising extracting ginsenoside from ginseng under a pressure of 20 to 110MPa as an active ingredient.

Health food composition according to another embodiment of the present invention is beta-glucanase (β-glucanase), pectinase (hemicellulase), before or at the same time before extraction under a pressure of 20 to 110MPa, Comprising ginseng extract prepared by a method comprising hydrolyzing ginseng by adding at least one enzyme selected from the group consisting of cellulase, arabinase and xylanase, as an active ingredient It features.

Using the present invention can be obtained a ginseng extract with a high content of ginsenosides. In particular, ginseng extract with a high content of Rg1, Rg2, Rg3 and Rb1 in ginsenosides can be obtained.

The present invention introduces an ultra-high pressure reaction in order to increase the extraction efficiency. "Ultra-high pressure" is a high pressure of more than 2 to 30,000 atm, using practical equipment can achieve about 10 million atm.

Since the ultrahigh pressure reactor is commercialized, it is possible to use a commercially available ultrahigh pressure reactor. For example, DFS-2L etc. of Toyo Japan High Pressure, Inc. can be used.

 In the production method of ginseng extract according to an embodiment of the present invention, the pressure is preferably 20 to 110MPa. If it is less than 20MPa extraction efficiency is insignificant, and if it exceeds 110MPa, the extraction efficiency increase is slowed down, which is uneconomical or the extraction efficiency is deteriorated for certain components.

In the case of ginsenoside Rg1, it is preferable to use at least one enzyme selected from the group consisting of pectinase, hemicellulase and cellulase. Most preferably pectinase. At this time, a preferable extraction pressure is 20 to 40 MPa or 90 to 110 MPa. Outside the above range, the extraction efficiency is lowered.

For ginsenoside Rg3, it is preferable to use at least one enzyme selected from the group consisting of beta-glucanase, pectinase, hemicellulase and cellulase. . In this case, the pressure at the time of extraction is preferably 40 to 90 MPa. Outside the pressure range, the extraction efficiency is lowered. Most preferably hydrolyzed to cellulose under a pressure of 70 to 90 MPa.

In the case of ginsenoside Rg2, it is preferable to use pectinase as a hydrolase. The pressure at the time of extraction is preferably 90 to 110 MPa. Outside the pressure range, the extraction efficiency is lowered.

In the case of ginsenoside Rb1, the pressure at the time of extraction is preferably 20 to 40 MPa or 90 to 110 MPa. Outside the pressure range, the extraction efficiency is lowered. The enzyme is preferably used a combination of pectinase, hemicellulose and cellulose, or pectinase alone. Most preferably, a combination of pectinase, hemicellulose and cellulose is used as the enzyme when the extraction pressure is 20 to 40 MPa, or pectinase alone is used as the enzyme when the extraction pressure is 90 to 110 MPa. .

As an example of cellulose which is an enzyme used to hydrolyze ginseng in one embodiment of the present invention, cellulose derived from the genus Tricholderma may be mentioned. Cellulase derived from the genus Tricholderma includes Econase CE (AB Enzymes GmbH, Darmstadt, Germany). The optimum pH of Econase CE is 4.0 to 5.5 and the optimum temperature is 55 ° C.

Other enzymes with cellulase activity include, for example, Rapidase (DSM, Delft, Netherlands) and Viscozyme (Novozymes, Dittingen, Switzerland). Rapidase is known as Aspergillus Niger or T. An enzyme derived from T. longibrachiatum , which has pectinase and hemicellulase activity in addition to cellulase activity. The optimum pH of Rapidase is 4.0 to 5.0 and the optimum temperature is 10 to 55 ° C. Viscozyme is an enzyme derived from the genus Asp. , In addition to cellulase activity, arabiase, beta-glucanase, hemicellulase and xylanase Has activity.

In another embodiment of the present invention, pectinase may be used as an enzyme for hydrolyzing ginseng. As an example of pectinase, Cytolase PCL 5 (Gist-Brocades, Seclin, France) is mentioned. Cytolase PCL 5 is Asph. As pectinase derived from Asp. Niger , the optimum pH is 2.5 to 5.0 and the optimum temperature is 10 to 55 ° C. Rapidase mentioned above also has the activity of pectinase and thus can be used as pectinase for hydrolyzing ginseng in this embodiment.

In another embodiment of the present invention, hemicellulose may be used as an enzyme for hydrolyzing ginseng. As hemicellulose, the above-mentioned Rapidase or Viscozyme can be used.

In another embodiment of the present invention, beta-glucanase may be used as an enzyme for hydrolyzing ginseng. An example of beta-glucanase is Ultraflo L (Novozymes, Dittingen, Switzerland). Ultraflo L is an enzyme derived from Humicola insolens with an optimal pH of 6 and an optimum temperature of 40 ° C. Since Viscozyme mentioned above also has beta-glucanase activity, Viscozyme may be used instead of Ultraflo L.

Other enzymes that may be used in another embodiment of the present invention include arabinase, xylanase, and the like.

 Ginsenoside Rg1 is an immune function enhancing, platelet aggregation inhibitory, antithrombin, memory and learning function, protein synthesis, anti-fatigue, antistress, central excitatory, vasodilation, high temperature environment and endogenous fever Protective actions against harmful stimuli such as substances, improvement of stressful behavioral disorders, anti-inflammatory and renal blood flow enhancement, neuronal cell survival rate, cholesterol metabolism, anti-inflammatory, liver cell proliferation and DNA synthesis promotion, liver protection It acts, stimulating the secretion of adrenal cortex stimulating hormone. Rg1 is preferably hydrolyzed to one or more enzymes selected from pectinase, hemicellulose and cellulose in ginseng. Especially preferably, hydrolysis is performed using pectinase under a pressure of 20 to 40 MPa or 90 to 110 MPa.

Ginsenoside Rg2 is ginsenoside which does not exist in white ginseng but exists only in red ginseng. Rg2 inhibits platelet aggregation, inhibits acetylcholine induced catecholamine secretion, inhibits intracellular calcium influx, antithrombin, memory decay, and smooth muscle cell proliferation. Hydrolysis by treating ginseng with pectinase can significantly increase the content of Rg2. In addition, the treatment of ginseng with cellulose or hemicellulose can also increase the Rg2 content to significantly higher levels.

Ginsenoside Rg3 also does not exist in white ginseng, but only in small amounts in red ginseng. Rg3 inhibits cancer cell metastasis, inhibits platelet aggregation and antithrombosis, inhibits liver injury, vascular relaxation, inhibits resistance of anticancer drugs, and protects nerves. When ginseng is hydrolyzed by treating pectinase, the content of Rg3 can be significantly increased. In addition, the treatment of beta-glucanase, cellulose or hemicellulose can increase the content of Rg3 to significantly higher levels.

Ginsenoside Rb 1 is contained in large amounts in both white and red ginseng. Rb1 inhibits central and mental stability, aggression, analgesic, anticonvulsant, hypercholesterolemic, anti-anxiety, cholesterol biosynthesis, DNA, RNA, protein and lipid synthesis, protein synthesis Promotes action, promotes neuronal survival, promotes acylcholine release, improves memory, inhibits platelet aggregation, inhibits lipid peroxidation, vasodilation, promotes cholesterol metabolism, anti-inflammatory, and protects liver injury. Ginsenoside Rb1 can increase its content to significant levels when hydrolyzed by treatment with pectinase, cellulose, hemicellulose.

In addition, ginsenosides such as Rg1, Rf, Re, Rd, Rc, Rb2 may also be selected from beta-glucanize, pectinase, hemicellulose, cellulase and / or arabinase and xylase in ginseng. By treating the enzyme with hydrolysis, its content can be significantly increased.

 In the preparation method according to an embodiment of the present invention, the hydrolysis process by enzyme treatment may be performed simultaneously with the extraction and / or concentration process.

In a manufacturing method according to another embodiment of the present invention, the hydrolysis process by enzyme treatment may be performed separately before the extraction process.

The health food composition according to one embodiment of the present invention may include ginseng or extract thereof prepared by any one of the above-mentioned methods as an active ingredient.

Health food composition according to another embodiment of the present invention is characterized in that it further comprises gamma cyclodextrin. Although ginseng has excellent efficacy, its unique bitter taste is recognized as a big problem. Ginseng-induced bitter taste in the cavity of gamma cyclodextrin can significantly reduce the bitter taste of ginseng. When the amount of gamma cyclodextrin is 100 parts by weight of liquid ginseng extract, specifically, ginseng concentrate, the content of gamma cyclodextrin is preferably 5 to 20 parts by weight. If the amount of gamma cyclodextrin is less than 5 parts by weight, the bitter taste masking effect is insignificant, and if it exceeds 20 parts by weight, the formulation stability and economy may be deteriorated. More preferred gamma cyclodextrin content ranges from 7 to 10 parts by weight.

Hereinafter, the present invention will be described in more detail with reference to one embodiment, but the scope of the present invention is not limited thereto.

Example

<Example 1 to Example 4: Preparation of red ginseng extract>

6 g of red ginseng powder purchased from Geumsan was suspended by adding 30 mL of phosphate buffer solution (50 mM, pH 5.0), and at 45 ° C., the pressure was increased to 0 MPa using an ultrahigh pressure reactor DFS-2L (Dongyang High Pressure, Japan). The extraction was carried out for 12 hours in the state of adjusting to Comparative Example 1), 30 MPa (Example 1), 50 MPa (Example 2), 80 MPa (Example 3), 100 MPa (Example 4). 300 mL of 80% ethanol was added to the extract to reflux for 3 hours to extract ginsenosides, and the reflux was concentrated to 30 mL.

Experimental Example 1 Measurement of Total Sugar, Acid Sugar, Polyphenol Content and Dry Matter Content

Total sugar, acid sugar, polyphenol content and dry weight of the concentrates obtained according to Comparative Example 1 and Examples 1 to 4 were measured. Polyphenol content was determined by the Folin-Denis method (Dewanto, V., Wu, X., & Liu, RH (2002b) .Processed sweet corn has higher antioxidant activity.Journal of Agricultural and Food Chemistry, 50, 4959 -4964.).

The contents of total sugar and acid sugar were measured by phenol-sulfuric acid method and menBlumenkrantz and Asboe-Hansen method (Blumenkrantz, N. and Asboe-Hansen, G. (1973) Anal. Biochem., 54, 484). . The results are shown in the table below.

sample pressure A shrine
(Total sugar) (mg / ml) Acid sugar
(Uronic acid)
(Μg / ml) Polyphenols
(Μg / ml) Dry weight (%) Comparative Example 1 0 (control) 103.2 ± 14.2 ^a 239.4 ± 37.9 ^b 346.3 ± 7.8 ^d 9.1 ± 0.2 ^a Example 1 30 MPa 113.1 ± 10.7 ^a 302.0 ± 113.7 ^ab 431.3 ± 17.1 ^a 8.2 ± 0.2 ^b Example 2 50 MPa 81.2 ± 4.3 ^b 185.9 ± 38.1 ^b 422.4 ± 12.4 ^a 6.3 ± 0.2 ^d Example 3 80 MPa 93.6 ± 19.0 ^ab 69.7 ± 15.2 ^c 398.1 ± 8.6 ^ab 6.3 ± 0.05 ^e Example 4 100 MPa 76.7 ± 27.9 ^b 418.8 ± 42.4 ^a 361.8 ± 5.3 ^c 7.6 ± 0.07 ^c

As shown in the table, the total sugar content was the highest 113.1mg / ml at 30MPa, but there was no significant difference from the control without pressure. The acidic sugar content showed the highest content of 418.8 µg / ml when 100 MPa was applied. The polyphenol contents were 431.3 and 422.4 µg / ml at 30-50 MPa pressure. The amount of buildings tended to decrease with pressure.

Experimental Example 2: Determination of ginsenoside content for Examples 1 to 4

1 g of each concentrate prepared according to Comparative Example 1 and Examples 1 to 4 was weighed out, and 50 mL of 70% ethanol was added thereto, extracted twice at 80 ° C shaking incubator, and filtered using Whatman # 1 paper. The filtrate was dried under reduced pressure using a vacuum concentrator and dissolved by adding 50 mL of distilled water. 2 mL of diethyl ether was added and mixed with 1 mL of the solution, followed by centrifugation at 1500 rpm for 10 minutes to remove diethyl ether, followed by mixing with 1.5 mL of water-saturated butanol, and then recovering the butanol layer. 1.5 ml of distilled water was added to the butanol layer, which was collected by repetition three times, and the mixture was centrifuged to remove the water layer. The butanol layer thus obtained was dried by injecting N ₂ gas at 40 ° C., and 0.5 mL of methanol was added thereto, followed by filtration through a 0.45 μm membrane filter, which was used as a sample for HPLC analysis. HPLC analysis was performed by ELSD using HPLC equipped with Prevail Carbohydrate ES 5u column (Alltech, USA) and performed under the conditions as indicated in Tables 2 and 3 (Gradient Table) below. On the other hand, ginsenoside analysis includes 14 standard materials (compound K, Rh2, Rh1, Rg5, Rk1, Rg2, Rg3, Rg1, Rf, Re, Rd, Rb2, Rc, A standard curve was prepared using Rb2) and the content was calculated from each peak area ratio.

Devices SP930D Solvent delivery pump
SDV50A Vacuum degasser and valve module
CTS 30 Column oven (Young-Lin Co. Ltd., Korea) Detector ELSD (GmbH, Germany) column Prevail Carbohydrate ES 5u, 250 × 4.6 mm (Alltech, USA) Oven temperature 35 ℃ menstruum A (Acetonitrile: Water: IPA = 80: 5: 15)
B (Acetonitrile: Water: IPA = 67: 21: 12) Injection volume 20 μl

Time
(min) Flow
(ml / min) % A % B Initial 0.8 90 10 28 0.8 15 85 35 0.8 20 80 45 0.8 25 75 50 0.8 10 90 51 0.8 0 100 57 0.8 75 25 58 0.8 90 10 65 0.8 90 10

The results are shown in Table 4 below.

Ginsenoside concentration (㎍ / mL) Comparative Example 1
(0 MPa) Example 1
(30 MPa) Example 2
(50 MPa) Example 3
(80 MPa) Example 4
(100 MPa) Compound K 0 0.00 0.00 0.00 0 Rh2 0 0.00 0.00 0.00 0 Rh1 0 0.00 0.00 0.00 0 Rg5 + RK1 0 0.00 0.00 0.00 0 Rg2 228.9 228.3 228.1 240.6 246.9 Rg3 0 0.00 0.00 0.00 0 Rg1 787.5 768.0 805.5 844.9 916.9 Rf 359.9 388.4 413.1 421.3 431.1 Re 2924.7 2909.3 2917.7 3475.9 2130.1 Rd 2453.7 2437.1 2820.5 2868.8 1499.5 Rb2 1275.8 1486.6 1689.6 2605.9 1558.7 Rc 3739.9 3388.9 3854.6 4787.0 2834.2 Rb1 5284.3 5550.1 6357.0 7831.1 5908.1 total 17054.7 17156.7 19086.1 23075.5 15255.5

As shown in the table, as the pressure increases, the ginsenoside content showed a tendency to increase, and in particular, the contents of the useful components Rg2 and Rg1 among the ginsenoside components also showed a tendency to increase with the pressure. The change in total ginsenoside content was 17054.7 μg / mL in Comparative Example 1 (control. 0 MPa), whereas it increased to 17156.7-23075.5 when pressure was applied. In addition, the content of Rg1 and Rb1, which are indicators of red ginseng products in the food industry, was 6071.8 ㎍ / mL in Comparative Example 1, whereas 30 MPa (Example 1), 50 MPa (Example 2), and 80 MPa (execution) Example 3) When the pressure was applied, it increased with the pressure of 6318.1, 7162.5, 8676.0 μg / mL, respectively, but decreased 6925.0 μg / mL for 100 MPa (Example 4). In addition, in the case of ginsenosides Rb1, Rb2, Rc, the content of these compounds is also important because compound K is transferred into the blood. These contents also increased to 10425.6-17217.4 ㎍ / mL when pressure was applied compared to the control (Comparative Example 1).

As such, when the red ginseng was extracted using the high pressure reaction, the content of ginsenosides of the red ginseng extract was increased.

<Examples 5 to 9: Preparation of extract by using a combination of high pressure and enzyme>

To 6 g of red ginseng powder purchased from Geumsan, 30 mL of phosphate buffer solution (50 mM, pH 5.0) was added and suspended. Then, 200 μl of each enzyme according to Table 5 was added thereto, and at 45 ° C., an ultrahigh pressure reactor DFS-2L (Oriental High Pressure) was added. , Japan) was extracted for 12 hours while the pressure was adjusted to 0, 30, 50, 80, 100 MPa. 300 mL of 80% ethanol was added to the extract to reflux for 3 hours to extract ginsenosides, and the reflux was concentrated to 30 mL.

enzyme Main active
Source Optimum pH Temperature (℃) Ultraflo L
(Novozymes, Dittingen, Switzerland) Beta-Glucane
Izu Humi-cola
Insole Lens (H
umicola
insolens) 6 40 Rapidase
(DSM, Delft, Netherlands) Pectinase, hemicellulose,
Cellulase Asp Niger & T. longibrachiatum 4.0-5.0 10-55 Viscozyme
(Novozymes, Dittingen, Switzerland) Arabica, Cellulose, Glucanase, Hemicellulase, Xylanase Asph. (Asp .) Genus 3.3-5.5 40-50 Cytolase PCL 5
(Gist-Brocades, Seclin, France) Pectinase Asph. Nigeo (Asp. Niger) 2.5-5.0 10-55 Econase CE
(AB Enzymes GmbH, Darmstadt, Germany) Cellulase Tricot Derma
Trichoder
ma) genus 4.0-5.5 55

Enzyme and pressure were prepared in Comparative Examples and Examples by varying as shown in Table 6 below.

pressure
enzyme 30 MPa 50 MPa 80 MPa 100 MPa control Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Ultraflo L Example 5 Example 10 Example 15 Example 20 Rapidase Example 6 Example 11 Example 16 Example 21 Viscozyme Example 7 Example 12 Example 17 Example 22 Cytolase PCL 5 Example 8 Example 13 Example 18 Example 23 Econase CE Example 9 Example 14 Example 19 Example 24

Experimental Example 3: Measurement of Total Sugar, Acid Sugar, Polyphenol, and Dry Matter Content

Total sugars, acidic sugars, polyphenols and dry matters in the same manner as in Experimental Example 1 together with the comparative examples without enzyme treatment for the extracts prepared according to Comparative Examples 2 to 5, and Examples 5 to 24 The amount was measured. Table 7 is a result of the total sugar content change, Table 8 is a result of the acid sugar content change, Table 9 is a result of the polyphenol content change, Table 10 is a result of the dry matter change.

Total sugar (mg / ml) enzyme 30 MPa 50 MPa 80 MPa 100 Mpa control Comparative Example 2
113.1 ± 10.7 ^bc Comparative Example 3
81.2 ± 4.3 ^a Comparative Example 4
93.6 ± 19.0 ^ab Comparative Example 5
76.7 ± 27.9 ^d Ultraflo L Example 5
148.8 ± 21.1 ^d Example 10
91.0 ± 11.8 ^ab Example 15
71.9 ± 7.5 ^a Example 20
118.4 ± 19.1 ^cd Rapidase Example 6
197.6 ± 5.6 ^fg Example 11
146.5 ± 9.0 ^d Example 16
161.0 ± 19.0 ^de Example 21
169.6 ± 25.4 ^ab Viscozyme Example 7
155.7 ± 11.1 ^de Example 12
179.3 ± 24.5 ^ef Example 17
188.6 ± 16.2 ^fg Example 22
197.0 ± 38.0 ^a Cytolase PCL 5 Example 8
187.9 ± 20.8 ^fg Example 13
157.9 ± 12.2 ^de Example 18
214.6 ± 21.0 ^g Example 23
141.0 ± 25.2 ^bc Econase CE Example 9
137.9 ± 23.2 ^cd Example 14
100.0 ± 6.8 ^ab Example 19
135.3 ± 6.1 ^cd Example 24
126.0 ± 4.03 ^bc

Acidic sugar (ug / ml) enzyme 30 MPa 50 MPa 80 MPa 100 MPa control Comparative Example 2
302.0 ± 113.7 ^abc Comparative Example 3
185.9 ± 38.1 ^ab Comparative Example 4
69.7 ± 15.2 ^ab Comparative Example 5
418.8 ± 42.4 ^b Ultraflo L Example 5
130.3 ± 30.3 ^ab Example 10
120.2 ± 97.4 ^ab Example 15
29.3 ± 8.8 ^a Example 20
320.8 ± 194.2 ^b Rapidase Example 6
1211.1 ± 162.0 ^gh Example 11
741.4 ± 114.7 ^ef Example 16
877.8 ± 83.5 ^ef Example 21
365.9 ± 25.0 ^b Viscozyme Example 7
600.00 ± 84.4 ^cde Example 12
1377.8 ± 38.1 ^hi Example 17
1266.7 ± 347.26 ^hi Example 22
1136.5 ± 104.6 ^a Cytolase PCL 5 Example 8
630.3 ± 367.7 ^def Example 13
731.3 ± 263.5 ^ef Example 18
1529.3 ± 86.2 ⁱ Example 23
546.7 ± 120.9 ^b Econase CE Example 9
867.7 ± 46.3 ^ef Example 14
362.6 ± 126.2 ^bcd Example 19
943.4 ± 319.4 ^fg Example 24
528.6 ± 98.7 ^b

Polyphenols (㎍ / ml) 30 MPa 50 MPa 80 MPa 100 MPa control Comparative Example 2
431.3 ± 17.1 ^b Comparative Example 3
422.4 ± 12.4 ^b Comparative Example 4
398.1 ± 8.6 ^ab Comparative Example 5
361.8 ± 5.3 ^d Ultraflo L Example 5
502.5 ± 25.0 ^c Example 10
503.2 ± 16.4 ^c Example 15
372.0 ± 28.9 ^a Example 20
378.7 ± 8.4 ^cd Rapidase Example 6
702.7 ± 27.1 ^gh Example 11
708.0 ± 25.2 ^h Example 16
709.4 ± 41.9 ^h Example 21
544.8 ± 24.4 ^a Viscozyme Example 7
651.2 ± 35.2 ^ef Example 12
849.0 ± 10.1 ^j Example 17
874.4 ± 40.6 ^j Example 22
579.0 ± 39.3 ^a Cytolase PCL 5 Example 8
662.5 ± 24.7 ^fg Example 13
750.3 ± 18.2 ⁱ Example 18
785.5 ± 12.3 ⁱ Example 23
419.0 ± 23.8 ^c Econase CE Example 9
583.9 ± 14.5 ^d Example 14
613.5 ± 3.7 ^de Example 19
664.6 ± 29.8 ^fg Example 24
475.3 ± 22.0 ^b

Dry weight (%) 30 MPa 50 MPa 80 MPa 100 MPa control Comparative Example 2
8.2 ± 0.2 ^d Comparative Example 3
6.3 ± 0.2 ^b Comparative Example 4
6.3 ± 0.1 ^b Comparative Example 5
7.6 ± 0.07 ^f Ultraflo L Example 5
8.9 ± 0.2 ^ef Example 10
7.2 ± 0.1 ^c Example 15
5.2 ± 0.1 ^a Example 20
8.6 ± 0.08 ^e Rapidase Example 6
12.3 ± 0.2 ^l Example 11
8.8 ± 0.4 ^ef Example 16
8.7 ± 0.1 ^e Example 21
10.7 ± 0.1 ^b Viscozyme Example 7
9.6 ± 0.1 ^g Example 12
10.6 ± 0.1 ⁱ Example 17
10.9 ± 0.2 ^jk Example 22
11.1 ± 0.08 ^a Cytolase PCL 5 Example 8
10.7 ± 0.2 ^ij Example 13
10.1 ± 0.1 ^h Example 18
11.2 ± 0.5 ^k Example 23
10.5 ± 0.06 ^c Econase CE Example 9
9.6 ± 0.1 ^g Example 14
7.9 ± 0.1 ^d Example 19
9.0 ± 0.1 ^f Example 24
9.2 ± 0.04 ^d

As shown in the table, Viscozyme hydrolyzate showed a tendency to increase the total sugar content as the pressure increases, ultraflo hydrolyzate tends to decrease the total sugar content as the pressure increases (Table 7). In the change of acidic sugar content, ultraflo and rapidase tended to decrease with increasing pressure at the pressure below 80 MPA, whereas the acidic sugar content of viscozyme and cytolase hydrolyzate increased with pressure. In case of 100 MPa, the acid sugar content tended to decrease. In particular, the content of acidic sugars, which are known to have various activities in red ginseng, was up to 418.8 ㎍ / ml when applied without enzymatic treatment. The content of was shown (Table 8).

Polyphenol content was 361.8-431.3 ㎍ / ml when only pressure was applied, whereas rapid contents of rapidase, viscozyme and cytolase were 544.8-709.4, 579.0-651.2 and 419.0-785.5 ㎍ / ml, respectively. Table 9). The dry matter content of the control was 6.3-8.2%, while the dry matter content of the enzyme hydrolyzate was 5.2-12.3%, and the dry matter content of the viscozyme and cytolase hydrolysates was relatively high (Table 10).

Experimental Example 4: Determination of Ginsenoside Content for Examples 5-9

Ginsenoside content was measured for each extract prepared in Examples 5 to 9. Ginsenoside content was measured in the same manner as in Experimental Example 2. The results are shown in the table below.

Ginsenoside 30 MPa (μg / mL) Comparative Example 2
(control) Example 5
(Ultraflo L) Example 6
(Rapidase) Example 7
(Viscozyme) Example 8
(Cytolase) Example 9
(Econase) Compound K 0.0 0.0 0.0 0.0 0.0 0.0 Rh2 0.0 0.0 0.0 0.0 0.0 0.0 Rh1 0.0 0.0 0.0 0.0 0.0 0.0 Rg5 + RK1 0.0 0.0 0.0 0.0 0.0 0.0 Rg2 228.3 256.4 308.3 0.0 378.5 254.0 Rg3 0.0 0.0 0.0 0.0 0.0 0.0 Rg1 768.0 1061.0 1275.6 854.4 1340.3 835.5 Rf 388.4 616.2 621.7 412.1 508.2 457.4 Re 2909.3 3869.4 4112.3 3209.9 2880.0 3236.3 Rd 2437.1 4482.5 6958.2 2593. 9 8070.6 3928.3 Rb2 1486.6 2348.9 3013.4 1662.7 2286.4 1948.5 Rc 3388.9 5295.0 3671.6 4388.0 1812.6 3948.4 Rb1 5550.1 7217.5 10914.3 6421.7 6927.9 6573.9

As shown in the table, the total ginsenoside content of the red ginseng hydrolyzate hydrolyzed at 30 MPa pressure was 17156.7 μg / ml in Comparative Example 2, while the content of enzyme hydrolyzate was 16948.8-30875.4 μg / ml content was shown, the highest content of the rapidase hydrolyzate (Example 6) was 30875.4 ㎍ / ml content. The content of Rg1 and Rb1, the indicators of red ginseng products, was 6318.1 ㎍ / mL in control (Comparative Example 2), while the lowest content of viscozyme (Example 7) hydrolyzate in enzyme hydrolyzate was 7276.1 ㎍ / mL. Rapidase (Example 6) The hydrolyzate showed the highest content of 12189.9 μg / mL. Rb1, Rb2, and Rc contents were 10425.6 µg / mL in the control (Comparative Example 2), while the hydrolyzate showed 11026.9-17599.3 µg / mL.

Experimental Example 5: Determination of ginsenoside content for Examples 10 to 14

Ginsenoside content was measured for each extract prepared in Examples 10 to 14. Ginsenoside content was measured in the same manner as in Experimental Example 2. The results are shown in the table below.

Ginsenoside 50 Mpa (µg / mL) Comparative Example 3
(control) Example 10
(Ultraflo L) Example 11
(Rapidase) Example 12
(Viscozyme) Example 13
(Cytolase) Example 14
(Econase) Compd K 0.0 0.0 0.0 0.0 0.0 0.0 Rh2 0.0 0.0 0.0 0.0 0.0 0.0 Rh1 0.0 0.0 0.0 0.0 0.0 0.0 Rg5 + RK1 0.0 0.0 0.0 172.3 193.3 132.0 Rg2 228.1 242.6 208.9 209.7 204.96 197.3 Rg3 0.0 0.0 940.7 943.5 804.6 711.9 Rg1 805.5 869.3 296.1 312.8 246.8 279.0 Rf 413.1 419.8 261.3 212.9 207.0 189.5 Re 2917.7 3117.7 3151.8 3546.9 2091.3 2988.5 Rd 2820.5 3628.2 5455.8 3574.6 5386.0 3678.7 Rb2 1689.6 2101.4 2173.3 2129.8 1710.8 2017.0 Rc 3854.6 4514.9 2934.3 4388.2 1530.0 4342.3 Rb1 6357.0 7075.2 8281.0 9227.6 5685.5 7733.4

As shown in the table, the total ginsenoside content of the red ginseng enzymatic hydrolyzate hydrolyzed under 50 MPa pressure was 19086.1 μg / ml for the control (Comparative Example 3), while the content of the enzyme hydrolyzate was 18060.3-24718.3 μg /. ml content was shown, and rapidase hydrolyzate (Example 11) had the highest content. The content of Rg1 and Rb1, which are indicators of red ginseng products, was 7162.5 ㎍ / mL in control (Comparative Example 3), whereas the lowest content of cytolase hydrolyzate (Example 13) was 5932.3 ㎍ / mL in enzyme hydrolyzate. The viscozyme hydrolyzate (Example 12) had the highest content of 9540.4 μg / mL. The Rb1, Rb2, and Rc contents were 11901.2 µg / mL in the control (Comparative Example 3), while the hydrolyzate showed 8926.3-15745.6 µg / mL. Viscozyme hydrolyzate (Example 12) showed the highest content (Table 11). Rg3 which was not measured at 30 MPa when reacted at 50 MPa was rapidase (Example 11), viscozyme (Example 12), cytolase (Example 13), econase (Example 14) in hydrolysates 940.7, 943.5, 804.6, 711.9 μg / mL was shown.

Experimental Example 6: Determination of ginsenoside content for Examples 15 to 19

Ginsenoside content was measured for each extract prepared in Examples 15 to 19. Ginsenoside content was measured in the same manner as in Experimental Example 2. The results are shown in the table below.

Fraction 80 Mpa (μg / mL) Comparative Example 4
(control) Example 15
(Ultraflo L) Example 16
(Rapidase) Example 17
(Viscozyme) Example 18
(Cytolase) Example 19
(Econase) Compd K 0.0 0.0 0.0 0.0 0.0 0.0 Rh2 0.0 0.0 0.0 0.0 0.0 0.0 Rh1 0.0 0.0 0.0 0.0 0.0 0.0 Rg5 + RK1 0.0 88.6 198.5 223.1 42.5 222.7 Rg2 240.6 196.6 205.1 216.2 0.0 211.8 Rg3 0.0 507.3 963.2 1131.9 985.8 1026.4 Rg1 844.9 251.3 324.2 362.5 175.7 365.6 Rf 421.3 172.9 269.4 227.5 206.3 177.0 Re 3475.9 2107.5 3113.5 4418.8 2859.9 4367.3 Rd 2868.8 2718.0 5840.0 4650.9 7891.8 5574.2 Rb2 2605.9 1516.5 2185.7 2585.2 2548.0 2857.99 Rc 4787.0 2967.9 2876.3 5405.6 1942.8 6187.5 Rb1 7831.1 3952.4 8089.3 10706.8 8374.8 10372.1

As shown in the table, the total ginsenoside content of red ginseng hydrolyzate hydrolyzed under 80 MPa pressure was 23075.5 μg / ml in control (Comparative Example 1), while the content of enzyme hydrolyzate was 14479.0-31362.6 μg / ml content was shown and econase hydrolyzate (Example 19) showed the highest content. The contents of Rg1 and Rb1, the indicators of red ginseng products, were 8676.0 ㎍ / mL in control (Comparative Example 4), whereas ultraflo (Example 15), rapidase (Comparative Example 16) and cytolase (Comparative Example 18) Degradates were 4203.7, 8413.5, and 8550.5 ㎍ / mL, respectively, lower than control (Comparative Example 4), but viscozyme (Example 17) and econase hydrolyzate (Example 19) were 11069.3 and 10737.7 ㎍ / mL, respectively. Comparative Example 4 showed a higher content. Rb1, Rb2, Rc content was 15224.0 μg / mL in control (Comparative Example 4), while hydrolyzate showed 8436.8-19417.6 μg / mL. Econase hydrolyzate (Example 19) showed the highest content (Table 12). The ultraflo hydrolyzate, in which Rg3 was not measured at 50 MPa, also contained 507.3 μg / mL at 80 MPa reaction (Example 15), rapidase (Example 16), viscozyme (Example 17), cytolase (Example 18), econase (Example 19) the hydrolyzate showed 963.2, 1131.9, 985.8, 1026.4 ㎍ / mL content, respectively.

Experimental Example 7: Determination of ginsenoside content for Examples 20 to 24

Ginsenoside content was measured for each extract prepared in Examples 20 to 24. Ginsenoside content was measured in the same manner as in Experimental Example 2. The results are shown in the table below.

Fraction Concentration (㎍ / mL) Comparative Example 5
control Example 20
Ultraflo L Example 21
Rapidase Example 22
Viscozyme Example 23
Cytolase PCL 5 Example 24
Econitase CE Rh2 0.0 0.0 0.0 0.0 0.0 0.0 Rh1 0.0 0.0 0.0 0.0 0.0 0.0 Rg2 246.9 317.0 277.9 325.1 438.6 306.5 Rg3 0.0 110.4 0.0 109.2 107.7 110.0 Rg1 916.9 1334.6 1106.3 1326.9 1535.5 1225.5 Rf 431.1 783.5 515.2 659.8 655.3 752.2 Re 2130.1 3711.9 2514.7 3583.5 3895.0 2973.2 Rd 1499.5 4292.4 3843.7 3571.5 6147.4 2750.3 Rc 2834.2 5568.6 3210.6 4598.0 6082.2 4637.6 Rb2 1558.7 3025.0 1760.5 2504.5 4144.0 2525.8 Rb1 5908.1 9042.7 6485.7 9407.9 17948.0 8376.9

As shown in the table, the total ginsenoside content of the red ginseng hydrolyzate hydrolyzed under 100 MPa pressure was 29367.5 µg / ml in control (Comparative Example 5), while the content of enzyme hydrolyzate was 38790.0-63335.7 µg /. It showed the ml content, cytolase hydrolyzate (Example 23) showed the highest content. The contents of Rg1 and Rb1, which are indicators of red ginseng products, were 9976.1 ㎍ / mL for control (Comparative Example 5), while the enzyme hydrolyzate was 11374.9-26731.1 ㎍ / mL, and the highest content of cytolase hydrolyzate (Example 23). Showed. Rb1, Rb2, and Rc contents were 17217.4 µg / mL in the control (Comparative Example 5), and rapidase hydrolyzate (Example 21) was 19318.0 µg / mL, which was the lowest in the enzyme hydrolyzate. Cytolase hydrolyzate (Example 23) showed the highest content of 46756.3 μg / mL. The content of Rg2 was 322.4 μg / mL in the control (Comparative Example 5), but the enzyme hydrolyzate showed a high content of 183.6-304.6 μg / mL, and the highest cytolase hydrolyzate (Example 23).

<Examples 25 to 44: A composition to which gamma cyclodextrin is added>

To 100 parts by weight of each red ginseng concentrate prepared according to Examples 5 to 24, 7 parts by weight of a gamma cyclodextrin corresponding to Cavamax® W8 food (WACKER, Germany) was further added.

Hereinafter, the composition of the present invention will be described by way of example, but it is not intended to limit the present invention but merely to explain in detail.

<Formulation Example 1: Soft Capsule>

Red ginseng concentrate prepared according to Examples 5 to 24 50mg, L-carnitine 80 ~ 140mg, soybean oil 180mg, palm oil 2mg, vegetable hardened oil 8mg, lead 4mg and lecithin 6mg, 400mg per capsule according to a conventional method Filled to prepare a soft capsule.

<Formulation Example 2: Tablet>

50 mg of red ginseng concentrate prepared according to Examples 25 to 44, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluidized bed dryer, followed by granulating with a tablet press by adding 6 mg of sugar ester. Tablets were prepared.

<Formulation Example 3: Granules>

 50 mg of red ginseng concentrate prepared according to Examples 5 to 24, 250 mg of anhydrous glucose, and 550 mg of starch were mixed and molded into granules by using a fluidized bed granulator, and then filled into fabric.

<Formulation example 4: drink system>

50 mg of red ginseng concentrate prepared according to Examples 25 to 44, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharides were mixed, and 300 ml of purified water was added thereto, and 200 ml were filled in each bottle. After filling the bottle sterilized for 4 ?? 5 seconds at 130 ℃ to prepare a beverage.

Formulation Example 5 Caramel Formulations

Red ginseng concentrate prepared in accordance with Examples 25 to 44 50mg, corn syrup (corn syrup) 1.8g, skim milk 0.5g, soy lecithin 0.5g, butter 0.6g, vegetable hardened milk 0.4g, sugar 1.4g, margarine 0.58g, And 20 mg of salt was mixed to form caramel.

Claims (15)

1) Red ginseng from the group consisting of beta-glucanase, pectinase, hemicellulase, cellulase, arabinase and xylanase Treating with one or more enzymes selected; and 2) A method for producing a high ginsenoside content of red ginseng, comprising extracting ginsenosides from the red ginseng under a pressure of 20 to 110 MPa. delete The method of claim 1, The enzyme treatment step and ginsenoside extraction step is a method for producing red ginseng extract, characterized in that at the same time. The method of claim 1, The ginsenoside is Rg1, wherein the enzyme is one or more enzymes selected from the group consisting of pectinase, hemicellulase and cellulase. The method of claim 4, wherein The enzyme is pectinase and the pressure is a method for producing red ginseng extract, characterized in that 20 to 40MPa. The method of claim 4, wherein The enzyme is pectinase and the pressure is a method for producing red ginseng extract, characterized in that 90 to 110MPa. The method of claim 1, The ginsenoside is Rg3, wherein the enzyme is at least one enzyme selected from the group consisting of beta-glucanase, pectinase, hemicellulase and cellulase. Method for producing red ginseng extract, characterized in that. The method of claim 7, wherein The pressure is a method for producing red ginseng extract, characterized in that 40 to 90MPa. The method of claim 8, The pressure is 70 to 90MPa, the enzyme is a method for producing red ginseng extract, characterized in that the cellulose. The method of claim 1, The ginsenoside is Rg2, the enzyme is pectinase, the pressure is a method for producing red ginseng extract, characterized in that 90 to 110MPa. The method of claim 1, The ginsenoside is Rb1, the pressure is 20 to 40MPa, and the enzyme is a method of producing red ginseng extract, characterized in that the combination of pectinase, hemicellulose and cellulose. The method of claim 1, The ginsenoside is Rb1, the pressure is 90 to 110MPa, the enzyme is a method for producing red ginseng extract, characterized in that the pectinase. 1) Red ginseng from the group consisting of beta-glucanase, pectinase, hemicellulase, cellulase, arabinase and xylanase Treating with one or more enzymes selected; 2) extracting ginsenosides from the red ginseng under a pressure of 20 to 110 MPa; and 3) A method of producing a health food composition comprising the step of preparing a health food composition by adding a red ginseng extract having a high ginsenoside content obtained in step 2). The method of claim 13, The health food composition, a method for producing a health food composition, characterized in that it further comprises gamma cyclodextrin. The method of claim 14, The gamma cyclodextrin is a method of producing a health food composition, characterized in that it is contained in an amount of 5 to 20 parts by weight when the liquid red ginseng extract is 100.