KR100861415B1 - Method of preparing cosmetics composition and cosmetics for anti-oxidation and skin-calming, prepared therefrom - Google Patents

Abstract

A method for preparing a cosmetic composition is provided to obtain an oriental cosmetic composition which shows anti-oxidation capability, elastase inhibitory activity and bacteriostatic capability without cytotoxicity and skin irritation. A method for preparing a cosmetic composition comprises the steps of: (a) hot water-extracting a mixture at a temperature of 80-100 deg.C for 8-48 hours after mixing myrrh, Sanguisorba officinalis, Acanthopanax Senticosus, Zanthoxylum piperitum, Eriobotrya japonica and Sophora flavescens and applying water thereto; (b) distilling a hot-water extract obtained from the step(a) and collecting it; and (c) mixing a collected distillate with chito-oligosaccharide. A cosmetic composition comprises an extract obtained by extracting 1.5-2.0 parts by weight of myrrh, 1.5-2.5 parts by weight of Sanguisorba officinalis, 1.5-2.5 parts by weight of Acanthopanax Senticosus, 1.5-2.5 parts by weight of Zanthoxylum piperitum, 1.0-2.0 parts by weight of Eriobotrya japonica and 0.1-1.0 part by weight of Sophora flavescens with 100 parts by weight of water and chito-oligosaccharide, wherein the content of the chito-oligosaccharide is 20-30 vol.% regarding the total composition.

Description

Method of preparing cosmetics composition and cosmetics for anti-oxidation and skin-calming, prepared therefrom

Figure 1 shows the results of MTT analysis by treating the cosmetic composition prepared according to an embodiment of the present invention to a human-derived keratinocyte cell line;

Figure 2 shows the results of measuring the antioxidant capacity of the cosmetic composition prepared according to an embodiment of the present invention using DPPH;

Figure 3 shows the results of measuring the elastase inhibitory activity of the cosmetic composition prepared according to an embodiment of the present invention;

Figure 4 shows the results confirming the bacteriostatic ability of the cosmetic composition prepared according to an embodiment of the present invention.

The present invention relates to a method for producing a cosmetic composition from herbal materials.

Chemically free radicals refer to species that exist independently with one or more unpaired electrons. In ordinary molecules, two electrons with opposite spin directions form a single electron pair and remain stable, but free radicals are generally unstable because they have unpaired active electrons and are therefore highly reactive. Short life

The production of free radicals in vivo occurs through a variety of pathways. Even during normal metabolism, 2% of the breathed oxygen is known to be converted to superoxide radicals. The cause of free radicals on skin cell and tissue damage after exposure to UV light suggests a consistent mechanism of damage to the action of UV light. The main physiological explanations are as follows.

(1) Free radicals are produced by the action of ultraviolet rays on the skin. (2) The skin has an antioxidant defense system that removes free radicals. However, such a defense system reaches its limit when the amount of ultraviolet rays is high and causes oxidation of cells and tissues. (3) As a result, major cellular components such as proteins, lipids, and DNA are damaged by free radicals, resulting in altered cellular function and ultimately promoting skin aging resulting in skin damage and disease.

There are three representative causes of wrinkles caused by free radicals in the skin. First, the destruction of skin antioxidants by UV light; second, the oxidation of cellular components such as lipid oxidation, protein oxidation, and DNA oxidation; and finally matrix metalloproteinase overexpression, collagen. It is a damage to the connective tissue caused by the breakage of chains, abnormal crosslinking, and the breakage of hyaluronic acid chains.

Free radicals produced by excessive UV exposure are known to cause various physiological damages that can promote wrinkle formation, as well as to stimulate the pigment cells present in the skin, accelerating the production of melanin pigments and darkening the skin. It can also cause severe inflammation and damage to skin immunity, depending on the extent and location.

Recently, as people's interest in skin increases, dermatologists are increasingly treating scars such as acne through laser dermabrasion to create clean skin.

Laser dermabrasion is mainly used to treat scars and wrinkles caused by acne or chickenpox, and the principle of treatment is a method of growing a lot of new tissues in the dermis by causing only 1 degree burn on the skin by laser light. Erbium yag lasers and carbon dioxide lasers are used for laser dermabrasion. Carbon dioxide lasers have a lot of thermal damage that causes a lot of damage to the surrounding tissues.

Erbium jag lasers have almost no thermal damage, resulting in less side effects such as erythema and pigmentation after treatment than carbon dioxide lasers. On the contrary, as it turns out that the scars can be filled well with some degree of thermal damage, it is recently used in combination with carbon dioxide laser rather than treatment with erb Yag laser alone. If you do not get satisfactory results with a single procedure, try several times, but have side effects such as hyperpigmentation after the procedure. In addition, since skin damage may occur due to thermal damage, the use of a non-irritating soothing lotion is necessary after the laser peeling to soothe the skin weakened by thermal damage.

Conventional herbal cosmetics contain ingredients extracted using hot water (heated water) extraction as main ingredients, which also have the disadvantage of extracting stimulating factors that can worsen damaged skin along with active ingredients. It is necessary to develop a manufacturing method that can.

The present inventors have faced such a problem, and developed a method for extracting the active ingredient in a purified state without the ingredients irritating the skin, thereby promoting the regeneration of the skin is damaged by the external heat of the cosmetic composition prepared In addition, it has excellent anti-oxidant ability, does not cause the transition of microorganisms even in long-term storage, so as to confirm that there is an effect of calming the skin without causing skin irritation during long-term use and completed the present invention.

It is an object of the present invention to provide a method for producing a cosmetic composition for extracting an active ingredient in a purified state without the components irritating the skin and a cosmetic composition produced thereby.

In order to achieve the above object,

According to one aspect of the invention,

i) mixing myrrh, fat milk, thorny scab, sancho, non-leafed and ginseng and extracting hot water by adding water;

ii) distilling and collecting the hot water extract obtained in step i); And

iii) a method of preparing a cosmetic composition comprising mixing the collected distillate of step ii) with chitooligosaccharides.

Hereinafter, the six herbal ingredients used in the preparation of the cosmetic composition of the present invention will be described in detail.

Myrrh Commiphora molmol Resin obtained from Engler or other similar plants ( Olivaceae Burseraceae). Myrrh's essential oils have cytotoxic and anti-inflammatory effects on fibroblasts in human gums. Acupuncture agents (1: 2) and myrrh inhibit the growth of various skin fungi and bacteria. Myrrh has astringent, analgesic, and cell regeneration effects.

Fat Milk Cucumber ( Sanguisorba officinalis L.) and dried roots and rhizomes of the same plant. The smell is weak, the taste is a little bit bitter. Fat milk has a tannin component, which indicates the fatal action by this component and reduces the production of secretions at the burn site. Applying cucumber extract to burns around 2 degrees improves in 2-3 days. Even if the life is dangerous to Mars 3 degrees, if you apply cucumber extracts while feeding the cucumbers or roots with fresh juice, it will improve within 20 days. In addition, applying oil extract to the skin irradiated with UVB protects the skin from UVB wrinkles and loss of elasticity. It also prevents pigmentation by UVB by inhibiting endothelin-converting enzyme-1α, which is involved in skin pigmentation. Fat milk sanguiin H-11 has anti-inflammatory action. Overall, the extract of fat milk has a good effect on astringent, hemostatic action, burns, dermatitis, eczema.

Prickly Pear Tree Acanthopanax senticosus The roots and rhizomes of the Maxim ( Agalaceae ) are dried. The main effect of prickly ginseng is tonic action of saponin. The saponin component prevents myocardial infarction and has hypoglycemic activity in hyperglycemic animals. Prickly pear extracts inhibit skin cell-dependent anaphylaxis and release of histamine. Liriodendrine also has pain relief. Oral administration of this substance reduces acetic acid-induced acute edema in the area of the foot. It appears to have been converted to syringaresinol, a hydrolyzate of Liriodendrin, which has anti-inflammatory and analgesic effects.

Sancho Zanhosylum piperitum DC. Or as much as possible the seeds of the fruit of other congenital plants (Rtaceae). Sanshoolfb and sanshoamid, which are contained in the sancho, are microscopic substances with local paralysis action. They have insecticidal action and detoxification for fish poisoning. α-sanshool and β-sanshool have almost the same degree of insecticidal and antimicrobial activity.

Loquat leaves are dried leaves of the loquat tree (Eriobotrya japonica). Loquat leaves contain glucose, sucrose, maltose, dextrin, amithrin, tartaric acid, etc. Among them, amithrin is one of the special components classified as vitamin B17. Benzoic acid, which is produced by the breakdown of amigrin, is effective for sterilization and pain relief.

Gosam is the thief's staff Sophorae flavescens Ait. (soybeans and Leguminosae) almost stripped roots. Ginseng extract has a strong antimicrobial activity against Propionibacterium acnes , the causative agent of acne. It also inhibits the growth of various skin fungi at 8% concentration. Sophoraflavone G has antimicrobial activity against Porphyromanas, Prevotella, Actinobacillus and Capnocytopaga. (+)-matrine and (+)-oxymatrine have central inhibitory effects, such as lowering body temperature, and glutamic acid blocking on neuromuscular specimens. (+)-Matrine also significantly reduces ear inflammation caused by croton oil.

Chitooligosaccharides are partial decomposition products of chitosan, soluble in water and easily absorbed. It is a wide range of highly functional bioactive substances with immune enhancement, antibacterial action, anti-inflammatory and anti-inflammatory action, skin soothing action and moisturizing action.

The cosmetic composition of the present invention is prepared by mixing the six herbal medicine extracts and chitooligosaccharides. Particularly, in the extraction of herbal medicines, the extract is heated again instead of simply obtaining the extract by conventional hydrothermal or solvent extraction. By collecting and obtaining the distillate, it can be said to be a manufacturing method which is different from the existing method.

In particular, in order to compensate for the disadvantage that the stimulating factor that may worsen the damaged skin together with the active ingredient during the extraction of hot water, the present inventors, such as the method of cooling and recovering the distillation component volatilized at a predetermined temperature or more during hot water extraction, A hydrothermal distillation fraction rich in essential oils, aromatics, and antioxidants was ensured, and the composition was confirmed to have antioxidant and skin soothing effects and completed the present invention.

Looking at the manufacturing method of the cosmetic composition of the present invention in detail,

Hot water extraction of step i) is preferably extracted for 8 to 48 hours at 80 to 100 ℃, preferably 10 to 12 hours at 90 to 95 ℃. The extraction efficiency is the highest in the above temperature range, the extraction efficiency is too low when extracted less than 8 hours and extraction more than 48 hours is not economical because it is excessive compared to the extraction efficiency.

The distillation of step ii) is performed by condensing the steam using a cooling tube of 4 to 10 ℃.

In addition, the method of the present invention may further comprise the step of centrifuging the collected distillate at 8,000 to 20,000 rpm for 10 to 50 minutes between the steps ii) and iii). The rpm and the range of time is appropriately selected to remove solids in the distillate, in the case of rpm is preferably 10,000 to 13,000 rpm. Further, the centrifugation process is preferably performed for 30 minutes at 4 ℃.

The distillate obtained after centrifugation to remove solids is mixed with chitooligosaccharide to prepare a cosmetic composition of the present invention. The chitooligosaccharide may be a low molecular weight chitooligosaccharide of 0.5% (w / v), but is not limited thereto. The chitooligosaccharide is preferably mixed to contain 20 to 30% by volume of the total cosmetic composition. If it is included in less than 20% by volume, the desired effect such as skin soothing, bacteriostatic, skin moisturizing is not sufficient, and when contained in more than 30% by volume is unstable formulation.

In addition, according to another aspect of the present invention,

Extract by adding 1.5 to 2.0 parts by weight, 1.5 to 2.5 parts by weight of fat milk, 1.5 to 2.5 parts by weight of oak bark, 1.5 to 2.5 parts by weight of anchovy, 1.0 to 2.0 parts by weight of non-leafed leaves, and 0.1 to 1.0 parts by weight of red ginseng. As a cosmetic composition comprising the extract and the chitooligosaccharide, the chitooligosaccharide may present a cosmetic composition comprising 20 to 30% by volume of the total composition.

As a result of MTT analysis on human keratinocytes using the cosmetic composition prepared by the method of the present invention, when 5% of the cosmetic composition of the present invention was treated, 106.16 ± 4.98% proliferation was compared to the untreated (control) group. Showed degree. In addition, 10% ± 0.74%, 106.05 ± 6.01%, and 98.51 ± 6.57%, respectively, at 1%, 0.5%, and 0.1% treatment showed a slight proliferative effect compared to the untreated group (see FIG. 1). This result means that skin irritation due to cytotoxicity is not induced even after long-term use of the cosmetic composition of the present invention.

In addition, in the antioxidation experiment using DPPH, it was confirmed that the DPPH free radicals of the reaction system were reduced by 20.9 ± 2.23% and 12.15 ± 1.64% when treated with 5% compared to the untreated group (see FIG. 2). These results mean that when the cosmetic composition of the present invention is applied to cosmetics and the like, even when using a high content of 5 ~ 10% can show a skin sedation with a significant antioxidant capacity while having less cytotoxicity.

On the other hand, the results of testing the degree of inhibition of the elastase enzyme activity of the cosmetic composition of the present invention can be seen that the inhibitory activity of about 13.25% compared to the control group using only distilled water when the present composition 10% (see Fig. 3) .

In addition, the cosmetic composition of the present invention, as shown in Figure 4, after confirming the presence of bacteria (A) and fungi (B) by the method of culturing after plating on agar medium, bacteria even immediately after preparation or left for 30 days at room temperature (C) and fungi (D) was confirmed that the proliferation did not.

In addition, the cosmetic composition of the present invention was found to have almost no skin irritation even when performing a skin irritation test using a patch test (see Table 1) .To summarize these results, the cosmetic composition of the present invention is There is no skin irritation or toxicity, so it can be seen that it can be usefully used in the manufacture of cosmetics because it has safe and excellent antioxidant capacity and skin soothing action.

In addition, according to another aspect of the present invention, it is possible to present a cosmetic comprising a composition prepared by the above-described manufacturing method. The cosmetic composition of the present invention can be applied to a wide range of cosmetics used for the skin. For example, it can take various forms such as aqueous solutions, soluble systems, emulsions or powders, oils, gels, ointments, aerosols, water-oil two-phase systems, water-oil-powder three-phase systems and the like. That is, in the case of a basic cosmetic composition, it can be used in the various forms described above as a face cleanser, a lotion, an emulsion, a cream, a gel, an essence, a pack, a mask, and other types of cosmetics.

In addition, in the case of makeup cosmetics can be used as a wide range of cosmetics, such as a foundation, while in the case of bath products can be used as a whole body soap, facial soap and the like. The form of the cosmetic comprising the composition of the present invention is not limited to the above-mentioned type and is included in the scope of the present invention as long as it includes the composition of the present invention.

Cosmetics comprising the cosmetic composition of the present invention, depending on the use, as a component, water, alcohols, oils, surfactants, preservatives, flavorings, pigments, moisturizers, thickeners, water-soluble polymers, antioxidants, chelating agents, pH Modifiers, blowing agents, vitamins, amino acids, various drugs and additives can be blended.

Cosmetics containing a cosmetic composition according to the present invention can be prepared by a conventional method, the following formulation examples are merely to illustrate the invention, whereby the content of the present invention is not limited.

Example 1: Preparation of Cosmetic Composition

Myrrh 200g, fat milk 200g, 200 g of thorny oak, 200 g of acid, 15 g of non-leaf leaves and 5 g of red ginseng were sufficiently washed in purified water, and 20 L of water was added thereto, and the mixture was heated to a boiling water distiller for 10 hours at 95 ° C. to obtain a hot water extract. While heating this hot water extract to 95 degreeC, the natural product complex distillate was obtained using the cold water cooling tube of 4 degreeC. Specifically, the distillate was condensed by passing a vapor volatilized during hot water extraction through a cold water cooling tube and recovered with a hot water still. The obtained natural complex distillate was centrifuged at 4 ° C. for 30 minutes at 12,000 rpm to remove solids, and the supernatant was used for subsequent experiments.

On the other hand, each of the herbal medicines by the above method first to obtain the herbal extract, and then mixed to the same final concentration showed the same antioxidant effect.

The herbal extract obtained and 0.5% (w / v) of low molecular weight chitooligosaccharide were mixed at a volume ratio of 75:25 to prepare a cosmetic composition of the present invention.

Test Example 1: Human keratinocyte cell culture

HaCaT cell line, a human keratinocyte, was cultured in a 37 ° C., 5% CO ₂ incubator for this study. When the degree of culture as much as 85 to 90% of the culture vessel was shown, the cells were detached by trypsin treatment, counted, and subcultured at 5 × 10 ³ cells / Cm ² . Cells were cultured with 10% fetal calf serum (FBS, GIBCO, Cat. No., 26140-079, USA) and Delbecco's Modified Eagle Medium (DMEM, GIBCO) with 100 U / ml phenylcillin and 100 ug / ml streptomycin. , Cat. No. 11995-065, USA). 75 T-flask (NUNC, Cat. No. 156499, Danmark) was used for subculture, and 24 well plate (NUNC, Cat. No., 142475, Danmark) was used for cytotoxicity test.

Test Example 2: Cytotoxicity Test Using MTT

The MTT test using the human-derived keratinocyte cell line HaCaT was performed as an indirect assessment of skin irritation that may be caused by long-term use of the cosmetic composition. Human keratinocytes cultured in the manner described in Test Example 1 were counted using a heamacytometer at 5 x 10 ³ cells / well in 24 well plates and then aliquoted. After 48 hours of incubation in DMEM containing 10% FBS to grow as much as 25-30% of the surface of the culture vessel, the cosmetic composition of the present invention was replaced with FBS-free DMEM containing 0.1-10% and incubated for 24 hours. After incubation, 50 ul of a 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT, Sigma M5655, USA) solution (2.5 mg / ml) was added thereto, followed by further incubation for 3 hours. Subsequently, discard the cell culture medium, and 200 ul of dimethyl sulfoxide (DMSO, Sigma D2650, USA) was treated with 200 ul for each well, followed by stirring. Absorbance was measured at nm. The degree of cytotoxicity was expressed as a percentage based on the absorbance intensity of the control group using pure water. The results are shown in FIG.

The 5% treatment resulted in 106.16 ± 4.98% proliferation compared to the untreated (control) group. In addition, 102.3 ± 0.74%, 106.05 ± 6.01%, and 98.51 ± 6.57%, respectively, at 1%, 0.5%, and 0.1% treatment showed a slight proliferative effect. This result means that skin irritation due to cytotoxicity is not induced even after long-term use of the cosmetic composition of the present invention.

Test Example 3: Antioxidant Test Using DPPH

In order to investigate the degree of antioxidant activity of the cosmetic composition of the present invention, the compound 1,1-diphenyl-2-picryl hydrazyl (DPPH, Sigma D9132-1G, USA) which produces free radicals in ethanol and the cosmetic composition of the present invention It was confirmed how much the amount of free radicals produced by mixing in a proportion.

Briefly, 0.1 ml of test substance containing 0.5 ml of 0.1 mM DPPH solution made from ethanol and 0.5% to 10% of the cosmetic composition is added to 0.4 ml of ethanol. Vortex strongly for 10 seconds and store in a cool dark place for 30 minutes. Absorbance is measured at 517 nm using ELISA. The degree of antioxidant activity was expressed as a percentage based on the absorbance intensity of the control group using pure water and the results are shown in FIG.

As shown in FIG. 2, the DPPH free radicals of the reaction system were reduced by 20.9 ± 2.23% and 12.15 ± 1.64% when treated by 5% compared to the non-treated group. However, 0.5% treatment did not show a significant difference compared to the control. These results indicate that even when the cosmetic composition of the present invention is used at a high content of 5 to 10%, the cytotoxicity can be exhibited with significant antioxidant capacity while having less cytotoxicity.

Test Example 4: Elastase enzyme activity inhibition test

Elastase is known to be a major factor in the formation of skin wrinkles by being exposed to excessive UV and environmental stress or by the expression of normal aging. In order to confirm that the cosmetic composition of the present invention has the ability to inhibit the enzyme activity of elastase, a reaction buffer of 0.1 M of sodium phosphate (pH 7.5) /0.5M NaCl was added to a 96 well culture dish, and then 20 ul of elastase was added thereto. do. After adding 25ul of 10% of the composition of the present invention, 25ul of suc-AlaAlaAla is added and the absorbance is measured at 405 nm. The degree of inhibition of elastase enzyme activity was expressed as a percentage based on the absorbance intensity of the control group using pure water. The result is shown in FIG. When the composition was treated with 10%, the inhibitory activity was about 13.25% compared to the control using only distilled water.

Test Example 5: The bacteriostatic ability through the remaining live bacteria test

Since the skin after laser dermabrasion is much weakened, abnormal microbial transition is caused, which may cause problems such as secondary infection after laser dermabrasion. It was confirmed whether the cosmetic composition of the present invention had bacteriostatic ability to prevent microorganisms from living without preservative treatment. In order to confirm the number of viable bacteria in the cosmetic composition of the present invention, immediately after the composition was prepared and left for 30 days at room temperature, a viable cell number identification test was performed. Specifically, 0.1 ml of the composition stock solution of the present invention was smeared on a retinal agar medium (Difco, USA) and incubated at 30 ° C. for 48 hours to confirm the presence of bacteria, and 0.1 ml of Sabrowood dextrose agar medium (Difco, USA) and incubated for 72 hours at 25 ℃ to confirm the presence of fungi.

As shown in Figure 4, the presence of bacteria (A) and fungi (B) was confirmed by the method of culturing after plating on the agar medium immediately after the preparation of the cosmetic composition of the present invention, there was no live bacteria, 30 days at room temperature After standing, it was confirmed that bacteria (C) and fungi (D) did not proliferate.

Therefore, the composition of the present invention has a bacteriostatic ability to inhibit the transition of microorganisms without the addition of preservatives even at long-term storage at room temperature, thereby enabling the skin soothing effect by preventing secondary infection of the damaged skin and preventing inflammation.

Test Example 6: Skin Irritation Experiment

Most herbal extracts have less skin irritation than general chemicals, but there is a problem that may cause skin trouble if the skin harmful substances are not properly removed, thereby measuring the degree of skin irritation of the cosmetic composition of the present invention. In order to do this, a patch test was conducted on 11 subjects.

A patch containing 100ul of the composition and the control of the present invention was attached to a clean area inside the subject's arm and washed 48 hours later with running water to determine the degree of stimulation after 30 minutes. The negative control group was pure water, and the positive control group was 5% sodium laureth sulfate. As a result of evaluating the skin response by dividing the stimulus value into five stages, it was confirmed that the skin irritation of the present composition was 4.5 in the negative control group, 38.6 in the positive control group, and 9.1 of the composition of the present invention.

division Stimulation level Skin irritation average (%) Irritant judgment 4 3 2 One 0 Negative Control 0 0 0 2 9 4.5 One Positive control group 0 One 5 4 One 38.6 4 Composition 0 0 One 2 8 9.1 One

* Average skin irritation (%):

{(4 Stimulus Number × Number of Subjects) + (3 Stimulus Number × Number of Subjects) + (2 Stimulus Number × Number of Subjects) + (1 Stimulus Number × Number of Subjects) + (0 Stimulus Number × Number of Subjects) } × 100 / maximum stimulus value (4) × all subjects)

* Irritation determination:

1 (less than 10, minimal stimulation), 2 (10-20, mild stimulation) 3 (20-30, moderate stimulation), 4 (more than 30, severe stimulation)

Formulation Example 1 Preparation of Lotion (Skin, Lotion)

A lotion (skin, lotion) containing the composition of the present invention as described below was prepared according to a conventional method.

Composition 10 (Unit; Weight%) of the Invention

Glycerin 3.0

Butylene Glycol 2.0

Propylene Glycol 2.0

Polyoxyethylene Cured Castor Oil 1.0

Ethanol 10.0

Triethanolamine 0.1

Preservative traces

A small amount of spices

A small amount of pigment

Purified water balance

Formulation Example 2: Preparation of Massage Cream

Massage creams were prepared according to conventional methods.

Carboxy vinyl polymer 0.45 (unit; weight%)

Triethanolamine 0.9

Glyceryl Stearate 1.5

Cetyl Alcohol 2.0

Fiji-30 Glyceryl Cocoate 1.0

Fiji-80 Glyceryl Cocoate 1.0

Sodium coco ampopropionate 4.0

Composition 3.0 of the present term

Preservative

Spices

Pigment amount

Purified water balance

Formulation Example 3 Preparation of Soap

Soaps containing the compositions of the present invention were prepared according to conventional methods.

Palm oil fatty acid 1.5 (unit; weight%)

Titanium Dioxide 0.5

Ethylenediaminetetraacetic acid disodium 0.15

Composition 0.3 of the present invention

Preservative

Spices

Pigment amount

Soap Chip Base Balance

As described above, according to the preparation method of the present invention, the herbal cosmetic composition having no cytotoxicity, having antioxidant capacity, inhibiting elastase activity, and having bacteriostatic ability but no skin irritation in a purified state It can be manufactured simply.

Claims (9)

i) mixing myrrh, fat milk, viburnum, sancho, non-leafed and ginseng and extracting hot water by adding water; ii) distilling and collecting the hot water extract obtained in step i); And iii) mixing the distillate collected in step ii) with chitooligosaccharide. The method of claim 1, wherein the hot water extraction of step i) is performed at 80 to 100 ° C for 8 to 48 hours. The method of claim 1, wherein the distillation of step ii) is carried out by condensing steam using a cooling tube of 4 to 10 ℃. The method of claim 1, further comprising centrifuging the collected distillate at 8,000 to 20,000 rpm for 10 to 50 minutes between steps ii) and iii). The method of claim 1, wherein the chitooligosaccharide of step iii) is mixed to include 20 to 30% by volume of the total cosmetic composition. Extract by adding 1.5 to 2.0 parts by weight, 1.5 to 2.5 parts by weight of fat milk, 1.5 to 2.5 parts by weight of oak bark, 1.5 to 2.5 parts by weight of anchovy, 1.0 to 2.0 parts by weight of non-leafed leaves, and 0.1 to 1.0 parts by weight of red ginseng. A cosmetic composition comprising an extract and a chitooligosaccharide, wherein the chitooligosaccharide is a cosmetic composition comprising 20 to 30% by volume of the total composition. The cosmetic composition according to claim 6, wherein the chitooligosaccharide has a concentration of 0.5% (w / v) chitooligosaccharide solution. The cosmetic composition according to claim 6, which has antioxidant activity, elastase inhibitory activity and bacteriostatic activity. Cosmetics comprising the composition of claim 6.

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