KR0180859B1 - Processing method of dietary fiber - Google Patents

Abstract

The present invention relates to a method for producing plant fibers having a high content of whiteness and indigestible components. More specifically, the present invention is to prepare a heat treated dextrin (dextrin) with excellent whiteness by using a starch material, and then mixed with an indigestible food polymer material to dry, high content of whiteness and indigestible components It relates to a method for producing plant fibers. The plant fiber material produced by the method of the present invention is somewhat more advantageous in white cotton than the plant fiber material made of underground starch, especially potato starch, and reaches the large intestine in an ovarian state, and has a formal function, and cholesterol in serum. And since it has been found to be effective in lowering the triglyceride value and inhibiting the increase in blood sugar and insulin secretion, it is expected that the field of various foods including beverages, ice cream, bread, sweets, etc. that can be applied through this.

Description

[Name of invention]

Method for producing plant fiber with high content of whiteness and indigestible components

Detailed description of the invention

The present invention relates to a method for producing plant fiber having a high content of whiteness and indigestible components. More specifically, the present invention is to prepare a heat treated dextrin (dextrin) with excellent whiteness by using a starch material, and then mixed with an indigestible food polymer material to dry, high content of whiteness and indigestible components It relates to a method for producing plant fibers.

In general, the heat treatment dextrin is prepared by high temperature heat treatment of starch containing a certain proportion of water in the presence or absence of acid. At this time, the starch molecule is hydrolyzed and optionally repolymerized to have a water-soluble complex structure containing a certain amount of non-digestible moieties, and the main component is glucose having 1 → 4, 1 → 6 glycosidic linkage as a main component. And trace amounts of 1 → 3 and 1 → 2 glycoside bonds are also present.

In recent years, as the standard of living improves, the dietary lifestyle is changed westernly, and the intake of fiber is decreasing. Therefore, the deficiency of fibrin may be one of the causes of the adult disease, causing new attention due to the fiber. Among them, plant fiber and various oligosaccharides are widely used as materials for improving the functionality of foods such as constipation improvement as examples of food materials having a bioregulatory function. In addition, consumption of processed foods, cooked foods, and fast foods is rapidly increasing, and consumers' desires are changing from nutrient-filled diets to health-oriented foods aimed at preventing nutritional disorders and various adult diseases caused by food habits. Among them, the demand for low-calorie foods is strong among adults, especially young women.

In general, plant fiber can be classified into vegetable and animal, and may be divided into water-soluble or insoluble depending on solubility in water. Also included herein are polydextrose as synthetic plant fiber. Since these fibers have a structure of fibrous form in which glucose and its derivatives or sugars other than glucose are bound to each other, they are complex and difficult to digest even if consumed in the body (digestible) or are released into the body. It is known to be effective.

On the other hand, these indigestible substances such as plant fibers and oligosaccharides exhibit various effects in the digestive tract and exert a physiological effect on the living body. For example, the water-soluble plant fibers in the upper digestive tract, the rate of plant movement It is effective in lowering and delaying absorption of nutrients. The delay in the absorption of sugar exhibits the effect of reducing insulin levels by suppressing the increase in blood glucose levels, and also reduces the cholesterol in the body by lowering the cholesterol in the serum by promoting the secretion of bile acids. In addition, the characteristics of these indigestible substances are not digested and absorbed in the small intestine, but reach the large intestine so that some of the plant fibers are degraded by enterobacteriaceae to produce short chain fatty acids. Acidification of the intestinal environment by short chain fatty acids has the effect of intestinal action and colon cancer prevention, and the absorbed short chain fatty acids are metabolized and converted into energy and inhibit cholesterol synthesis.

These plant fibers are defined as the total of indigestible components in plants that are not digested by human digestive enzymes, and in particular, water-soluble plant fibers have attracted attention as functional food materials because they have a strong physiological action as described above. These water-soluble plant fibers include natural gums such as guar gum, glucomannan, and pectin, but most of them are highly viscous and difficult to consume in large quantities alone. In addition, there is a problem in the food manufacturing to add to the processed food, there are many difficulties in terms of processing.

In addition, in the case of starch material, alpha-starch, starch derivative, glucose, powdered starch, maltodextrin, etc., which are processed products of starch, are used in various processed foods as food materials. However, most of these starch processed products have an indigestible component content of 5% or less and a calorie value of 3.9 kcal / g or more. In starch systems, what can be expected as a plant fiber and a low calorie food material is limited to heat-treated dextrins. .

The plant fiber is attracting attention as a functional food material used as various functional foods, that is, plant fiber beverages, fermented milk products, baking, confectionery additives, and is expected to be widely applied in the future. On the other hand, there have been a number of limitations to the method of making plant fibers using starch materials:

EP 368451B1 discloses a process for producing dextrins containing plant fibers by dissolving sodium dextrin in water and wearing alpha-amylase, and acting on alpha-amylase and adding hydrogen. Method prepared by wearing and co-glucosidase alone and co-working with beta-amylase, chromatography with alpha-amylase and glucoamylase, filtration, deodorization and desalting, followed by strong acidic cation exchange resin The present invention discloses a method for collecting plant fiber powder by chromatography, and a method for producing plant fiber by mixing and roasting starch alone or monosaccharides and oligosaccharides with starch before producing roasted dextrin.

In addition, EP 477089A1 roasts potato starch in the presence of hydrochloric acid, and then reacts with alpha-amylase, reacts with glucoamylase, and then filters and removes plant fiber powder from the reactants by treatment with ion exchange resin chromatography or organic solvents. Disclosed is a method of manufacturing.

EP 487187A1 and USP 5,430,141 disclose a process for producing low calorie foods, comprising low calorie maltodextrin obtained by adding inorganic acid to potato starch, dissolving the heated dextrin in water and applying alpha-amylase.

In USP 5,264,568, the aqueous solution of Basodextrin prepared is adjusted to pH 7.0 to 8.5, and the solution is prehydrolyzed by adding alpha-amylase derived from B. licheniformis, followed by pH of 5.5 to Adjust to 6.5, hydrolyze again by raising the temperature, autoclave at the temperature above the boiling point of this solution, cool, add the above alpha-amylase again, and autoclave again at the temperature above the boiling point of the hydrolysate and roast. A method for preparing a hydrolyzate of dextrin is disclosed.

US Pat. No. 5,358,729 discloses a method for producing an indigestible dextrin with less coloring matter or an irritant odor with 60% or more of an indigestible portion by adding hydrochloric acid to starch and heating it to 120 to 200 캜 using an extruder.

In EP 535627A1, EP 535627A1 and USP 5,364,652, hydrolysis of starch dextrin prepared by adding hydrochloric acid to starch is hydrolyzed with alpha-amylase and glucoamylase, and at least half of the resulting glucose is separated and removed. A method for producing a novel indigestible dextrin with 75% or more and less coloring matter or irritant smell is disclosed.

However, the above-described methods of the prior art have not only caused the reaction not to occur uniformly, but also have a problem that the discoloration of the starch causes the commercialization of the manufactured plant fiber, and the content of the indigestible component is low. .

As a result, the present invention significantly improves the above-described problems of the prior art, and the present invention is as follows when the present invention is compared with the prior art.

EP 368451B1 sprays potato starch with liquid hydrochloric acid, and then predrys and roasts the mixture to cause discoloration of the starch. In the present invention, corn starch is placed in a dryer and hydrochloric acid is added in the form of a gas with hot air. The heat-treated dextrin prepared while preventing discoloration of the starch to the maximum was used for the production of plant fibers. In addition, in the prior art, monosaccharides and oligosaccharides were mixed with starch before preparing the roasted dextrin, but in the present invention, a dry amount of a functional additive was simply mixed with a V-mixer or the like in a heat-treated dextrin. After the enzymatic reaction was carried out.

In addition, EP 477089A1 is aged by spraying a certain amount of hydrochloric acid solution on potato starch, pre-dried by flash dryer, roasted, hydrolyzed with alpha-amylase, glycosylated with glucoamylase, and then adjusted to pH to control glucoamylase. Activated and passed through an alkali metal-type strong acid cation exchange resin or vacuum-dried the needle formed by using ethanol to prepare a plant fiber or by vacuum drying the precipitate formed by using ethanol to prepare a plant fiber. However, in the present invention, white dextrin reacted with high temperature hot air in the enzymatic reaction in order to prevent uniform reaction and discoloration of starch as much as possible, and transglucose to increase the content of indigestible component of plant fiber after the reaction of glucoamylase The content of the indigestible component could be further increased through process improvement such as adding a certain amount of a sidase and using a separation resin having a uniform particle size to separate monosaccharides in the reaction solution.

EP 487187A1 and USP 5,430,141 are sprayed and mixed with starch hydrochloric acid solution in starch, mixed with pre-drying and heat treatment, followed by addition of alpha-amylase, followed by decolorization filtration and alpha-amylase and glucose to the aqueous solution of Basodextrin. After reacting amylase and filtering and purification, low calorie dextrin was prepared by separating the digestible portion using an alkali metal ion exchange resin or ethanol. However, in the present invention, white dextrin prepared by injecting gaseous hydrochloric acid into starch together with high temperature hot air in the starch is used for enzymatic reaction in order to uniformize the reaction and prevent discoloration of starch. In order to further increase, an indigestible functional additive was simply mixed dry with white dextrin before the enzymatic reaction, and various enzymes were reacted with the mixture, and then a separated resin column having a uniform particle size and an organic solvent were used again.

In addition, USP 5,264,568 sprayed hydrochloric acid solution on potato starch, aged, pre-dried and alpha-amylase in heat-treated basodextrin, which was first hydrolyzed at low temperature for a long time, and then the reaction solution was placed in an autoclave. In the present invention, various functional additives were dried to increase the content of the indigestible component of plant fiber in the white dextrin prepared through the homogeneous reaction through high temperature heat products. After mixing and hydrolyzing with alpha-amylase, reacting with glucoamylase and transglutosidase, an ion exchange resin and an organic solvent of uniform particle size were used to remove monosaccharides.

Meanwhile, in USP 5,358,729, corn starch was mixed in a ribbon mixer, sprayed with 1% hydrochloric acid, and then pre-dried with a flash dryer and extruded at 120 to 200 ° C. using a twin extruder. In the present invention, white dextrin prepared through hot air is reacted with various enzymes.

In EP 535627A1, EP 535627A1 and USP 5,364,652, starch is sprayed with hydrochloric acid and aged for about 8 hours. The pH was lowered to stop the action of the enzyme, and then the glucose portion was separated by 50% or more using a strong acid cation exchange resin column. On the contrary, in the present invention, gaseous hydrochloric acid is added to the starch together with high temperature hot air in order to produce the indigestible component of the dextrin itself and prevent discoloration of the starch, and also neutralize the dextrin in advance with the reaction pH of alpha-amylase. The step of adjusting the pH was omitted, and in addition, the heat-treated dextrin was mixed with an indigestible functional additive, treated with alpha-amylase and inactivated by lowering the pH, followed by glucoamylase and the indigestible component of the reaction solution. In order to increase the content, transglucosidase was further reacted to remove digestible parts such as monosaccharides with organic solvents such as ion exchange resin and ethanol of uniform particle size after activated carbon purification and filtration.

In addition, the dextrin prepared through the general rotary roaster may take a considerable overload in the purification process such as decolorization of the sugar solution after the reaction uniformity and starch-specific whiteness fall, but the heat treatment dextrin used in the present invention is a high temperature The desired purpose could be achieved by using hot air and reacting gaseous hydrochloric acid with starch to prevent discoloration of the starch by oxidation. In addition, in the last step in the preparation of dextrin, it was possible to advance the hydrolysis immediately without neutralizing the pH of the aqueous solution of dextrin by pre-neutralizing to the pH level of alpha-amylase.

After all, the main object of the present invention relates to a method for producing a plant fiber having a high content of whiteness and indigestible components.

Still another object of the present invention is to provide a plant fiber having a high content of whiteness and indigestible components produced by the electric method.

Hereinafter, the present invention will be described in more detail.

In the present invention, various functional additives are mixed with dextrin prepared by heat-treating starch as a raw material used as plant fiber beverage and various functional health food materials at high temperature as a raw material, and separating and removing digestible monosaccharides from sugar solution through enzymatic reaction. Prepare the fibers.

In other words, after pre-drying the general starch with hot air, a certain amount of anhydrous hydrochloric acid is reacted with starch particles with high temperature hot air uniformly and heat-treated for a certain time, and then the heat-treated dextrin is added with an indigestible functional additive. Mix. Here, hot water is added to dissolve the heat-treated dextrin mixture, alpha-amylase, beta-amylase, glucoamylase and transglucosidase, and the like are then desalted through activated carbon filtration and ion exchange resin, and the separation resin having a uniform particle size. And by using an organic solvent such as ethanol to remove the digestible parts such as monosaccharides to prepare plant fibers.

The starch used in the present invention is a commercially available general starch, and may use ground starch such as corn starch, waxy corn starch and wheat starch, and underground starch such as potato starch, sweet potato starch and tapioca starch. For example, relatively low-cost corn starch may be used. The quality level of raw starch is generally acceptable if it is on the market.

Hereinafter, a method for producing the plant fiber of the present invention will be described according to the process.

[Step 1]

Preparation of Heat Treated Dextrin

General starch of 10 ± 2% moisture level is placed in a dryer and pre-dried at 80 to 100 ° C., preferably 85 to 95 ° C. by hot air, and has a water content of about 4 to 7%, preferably 5 to 6%. At the time, 0.04 to 0.1% by weight, preferably 0.05 to 0.07%, of gaseous hydrochloric acid is added together with the hot air introduced. As a heat source, it heat-processes at 110-180 degreeC, Preferably it is 130-140 degreeC using steam, and makes it react for 60 to 90 minutes, Preferably it is 70 to 80 minutes. In addition, the sample is taken to analyze the flow rate, the content of soluble substances, etc. in order to determine whether the conversion to dextrin is appropriate, the flow rate is preferably about 50 to 55ml, the content of soluble substance should be at least 86%. .

Subsequently, upon confirmation of the process, the reaction is cooled and mixed in a dry state by adding ammonium bicarbonate or anhydrous sodium bisulfice as a neutralizing agent to maintain a pH of 5.5 to 6.5. And cool for another 30 minutes. In this case, the amount of the neutralizing agent is preferably 0.01 to 0.10% by weight relative to starch in the case of ammonium bicarbonate, 0.1 to 0.5% by weight in the case of anhydrous sodium bisulfite, and separated through a six mesh screen to prepare plant fibers We use for. Table 1 summarizes the analysis method and analysis results of the heat-treated dextrin prepared according to the process of the present invention.

* D.E. (dextrose equivalent) was analyzed using Fiske Co., Ltd., U.S.A.

** Reference: Food Code Analysis (Food Industry Association, pp675-677, 1994)

[Step 2]

Preparation of plant fiber with high content of indigestible component

First, an indigestible functional additive is mixed with the heat-treated white dextrin. At this time, the additives used are gum arabic, hydrolyzate of gum, wheat bran, oat bran, corn fiber, soybean plant fiber, rice bran hemicellulose, glucomannan, guar gum, Guar gum enzyme digests, locuat bean gum, xanthan gum, pectin, carrageenan, sodium alginate, curdlan and pullulan, Preferably, 1 to 20% by weight of arabic gums, guar gum enzymatic products, galactomannan, rice bran hemicellulose, soybean plant fiber, corn fiber, etc., which have relatively low viscosity when dissolved in water, are preferably 5 to 20% by weight. Mix 10% by weight. Addition method is to add the dextrin and functional additives to the v-mixer, ribbon- blender and kneader (kneader) and mix uniformly for 10 to 30 minutes by simple blending.

The mixture is then dissolved in a concentration of 30 to 50% by weight, preferably 40 to 45%, using preheated hot water and the pH is checked. At this time, the dextrin used in the present invention is adjusted to pH 5.5 to 6.5 through neutralization in advance in the manufacturing process, there is no need to adjust the pH separately. To this is added commercially available alpha-amylase from 0.05 to 0.5% by weight, preferably from 0.1 to 0.2% by weight relative to the heat treated dextrin, wherein the temperature of the solution is maintained at 80 to 100 ° C, preferably 90 to 95 ° C. At this temperature, it is heated for 30 to 120 minutes, preferably 60 to 120 minutes. Such enzymatic treatment can remove the irritating odor and undesirable taste peculiar to the heat treatment dextrin. Activated carbon was added to the sugar solution thus obtained and heated to inactivate the enzyme. The mixture was mixed with cations and anions at a predetermined ratio, followed by general purification processes such as filtration, decoloration, and desalting, and then concentrated in a vacuum concentrator. It can be dried in a dryer to obtain a plant fiber material.

In addition, in the present invention, in order to further increase the content of the indigestible component of the plant fiber, the alpha-amylase may be acted and subjected to enzymatic treatment again. That is, a process of reacting transglucosidase and beta-amylase after the action of alpha-amylase may further be included. After transglucosidase and beta-amylase, the pH was lowered to 3 or less after the action of alpha-amylase and left for 10 minutes at 95 ° C or higher to deactivate the enzyme, and then the pH was adjusted to 5.5 to 6.5 with 1N sodium hydroxide solution. The temperature of the sugar solution is adjusted to 55 to 60 ° C, and then 0.05 to 0.5% by weight of the white dextrin is added to react for 24 to 72 hours. Preferably, 0.05 to 0.2% by weight is added for 36 to 48 hours. The reaction further complicates the molecular structure of the plant fiber, while the small molecules, such as glucose produced, are repolymerized into limiting dextrins, which are no longer degraded by functional polymers and enzymes. After a certain period of time, activated carbon treatment inactivates transglucosidase and beta-amylase. Also, rather than reacting the two enzymes separately, it is rather desirable to process them simultaneously. The sugar solution thus obtained is treated with activated carbon, filtered, desalted and decolorized through an ion exchange resin, concentrated to 50% concentration, and dried with a spray dryer to prepare a plant fiber material.

The present invention also includes a process for preparing plant fibers having an increased content of indigestible components by removing monosaccharides which form most of the digestible substances in the above-mentioned sugar solution. First, when the sugar solution concentrated at 50% concentration is passed through the separation column of the strong acid cation, the larger molecular weight and the indigestible substance come out first, and thus it is possible to separate from the monosaccharide having digestibility. The strongly acidic cation exchange resin that can be used may be generally commercially available, and examples of specific resins include Amberlite IR-118, IR-120B (Organo Co., Ltd., JAPAN), XFS-43281.00, 43278.00 (Dow). Chemical Co., Ltd., USA), Diaion UBK-530K, Diaion UBK-530, Diaion UBK-535, Diaion FRK-111, Diaion FRK-121, and Diaion FRK-131 ( Mitsubishi Kasei Technoengineers Ltd., JAPAN) and the like. Preferably, the diion UBK series and the FRK series, which are widely used as separation resins, are used, and this resin does not use strong acid chemicals such as hydrochloric acid. It is much more advantageous in handling because it is liquid. In order to improve the separation between the indigestible polymer material portion and the monosaccharide, it is preferable to adjust the speed at the time of column passing, depending on the resin used. The liquid permeability is preferably in the range of SV = 0.1-0.5, in order to improve the separation efficiency more preferably in the range of 0.3 to 0.4, if too fast it is not easy to separate the polymer and monosaccharides. When passing through, the temperature of the column is adjusted to 30 to 70 ℃, preferably 50 to 60 ℃, if the temperature is too low, it is difficult to separate and the viscosity of the liquid increases, causing a bad effect on the resin, if the temperature is too high This will affect the quality of the product.

On the other hand, in order to further increase the content of the indigestible component of the plant fiber through the separation of the indigestible polymer and monosaccharides, it may be recovered by inducing precipitation of the polymer material by adding an organic solvent to the sugar solution. At this time, the organic solvent that can be used is limited to ethanol because it is a food material. 95% ethanol is added to the 50% concentration of the enzyme reaction solution prepared above, preferably 2 to 3 times the weight of the sugar solution, and when the ethanol is too small compared to the sugar solution, the precipitation of the polymer material is easy. Does not occur. Allow to stand overnight to improve the separation efficiency of ethanol solubles (monosaccharide fraction) and the precipitate produced. After removing the portion of the supernatant and dissolving the remaining precipitate in a certain amount of water, the remaining ethanol is completely removed through a vacuum concentrator. The sugar solution thus obtained is adjusted to 50% concentration, and then dried through a spray dryer to prepare powdered plant fibers.

In addition, the present invention also includes a method of dissolving a mixture of white dextrin and various functional additives, treating alpha-amylase followed by glucoamylase and transgulcosidase, and separating the existing monosaccharides into a separation resin and ethanol. . That is, after the reaction of alpha-amylase, the pH was lowered to 3.0 or lower, and the pH was again adjusted to 4.5, and then 0.05 to 0.5% by weight of glucoamylase, preferably 0.05 to 0.2, when the temperature of the solution was about 55 ° C. Add weight percent and react for 24 to 48 hours. This reaction breaks down the digestive part present in the solution into glucose and stops the action of glucoamylase by raising the temperature to about 90 ° C after the reaction. Then, after adjusting the pH again to about 5.5 and the temperature of the solution to about 55 ° C., respectively, 0.05 to 0.5% by weight of transglucosidase, preferably 0.05 to 0.2% by weight, and reacted for 24 to 48 hours. This reaction results in a complex molecular structure by repolymerization with the functional polymer added with the digestible glucose produced by the glucoamylase and the limit dextrin portion having enzyme resistance, thereby increasing the content of the indigestible component. The sugar solution prepared by the above reaction was treated with activated carbon to deactivate the enzyme, filtered through a glass filter, etc., and then subjected to a series of purification processes such as desalination through an ion exchange resin column, and then concentrated to about 50% by weight using a vacuum concentrator. do. According to the present invention, in order to further increase the indigestible component of this solution, as mentioned, the digestible monosaccharides are separated from the indigestible polymer by using diion UBK and FRK series separation resins or by ethanol precipitation. Plant fibers can also be prepared by concentrating the useful components and then spray drying.

Plant fiber material produced by the method of the present invention can be widely used in various foods, including beverages, ice cream, bread, sweets and the like. In addition, the plant fiber produced by the present invention is somewhat advantageous in the back road than the plant fiber material made of underground starch, in particular potato starch material, it is expected that the field of food can be further expanded through this.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only intended to explain the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples.

Example 1

Corn starch of 12% moisture was placed in a fluid bed dryer (Glatt GmbH GERMANY) and pre-dried at 93 ° C. while fluidizing with hot air. Then, when the water content is 5%, gaseous hydrochloric acid was added to the hot air, added 0.056% by weight relative to starch, and heat-treated at 135 ° C for 75 minutes. In order to confirm that dextrinization proceeded smoothly, a sample was taken and measured for physical properties. As a result, the fluidity was 55 ml, 95% soluble substance, and whiteness 88. After the process is confirmed, the reaction product is cooled and neutralized. When ammonium bicarbonate is used as a neutralizing agent, 0.067% by weight of starch and 0.3% by weight of starch when anhydrous sodium bisulfite are added are mixed in a dry state. The pH was maintained at 5.8, cooled for 30 minutes, and then separated through a 6 mesh screen to prepare a dextrin having an indigestible content of 47%, and used to mix it with a functional additive.

3 kg of the prepared heat treated dextrin was placed in a kneader (Kneader, Littleford Bros., Inc., GERMANY), and 5% by weight of gum Arabic (Colloides Naturels International, FRANCE) was added to the heat treated dextrin and mixed for 30 minutes. Hot water at 90 ° C. previously heated to 1 kg of this mixture was added to obtain a solution of 40% by weight of heat treated dextrin. Since dextrin was previously adjusted to about 5.8, which is the optimum pH of alpha-amylase, the pH was simply confirmed and hydrolysis was performed. The temperature of the solution was raised to 95 ° C, and commercially available alpha-amylase (Novo Nordisk Bioindustry Ltd., Thermomil 120 L, DENMARK) was added 0.2% by weight relative to the heat treated dextrin and allowed to react for 1 hour. 1 wt% of powdered activated carbon compared to dextrin was added to the enzyme reaction solution, and the enzyme was inactivated by heating and filtered using a filter paper (Whatman International Ltd., GF / B, GF / F, UK), followed by cation and anion exchange resin. Decolorization and desalting were carried out through a general sugar crystal process which is passed through the mixed resin column. Subsequently, the resultant was concentrated to 50% concentration using a vacuum concentrator, and then dried in a spray dryer to prepare plant fibers having excellent whiteness. The content of the indigestible component in the plant fiber thus obtained was 60% as determined by the HPLC method described in the Food Code, and the whiteness of the colorimeter was 85. For reference, the whiteness of the potato starch material plant fiber was 70, the content of the indigestible component is 50%.

Example 2

Corn starch of 12% of water was placed in a fluidized bed dryer and pre-dried at 90 ° C. while fluidizing with hot air. When the water content was 4.5%, gaseous hydrochloric acid was added to hot air applied to starch, added 0.067% by weight relative to starch, and heat-treated at 130 ° C. for 75 minutes. Samples were taken to determine the degree of reaction and the physical properties were measured. As a result, a flow rate of 67 ml (dissolved 75 g of dextrin in 107 ml of distilled water and analyzed with a standardized funnel), 80% soluble matter, and whiteness 89 was shown. After the process is confirmed, the reactant is cooled and neutralized.Ammonium bicarbonate is added as a neutralizing agent to 0.067% by weight of starch, uniformly mixed in the dry state, cooled for 30 minutes, and then separated through a 6 mesh screen to indigestible components. Dextrin having a content of 45% was obtained and used to mix it with the functional additive. 2 kg of the prepared heat treated dextrin was placed in a kneader, and 10% by weight of hydrolyzate of Arabian gum (Colloides Naturels International, FRANCE) was added to the heat treated dextrin and mixed for 30 minutes.

To 1 kg of this mixture was added hot water at 90 ° C. previously heated to obtain a solution of 40% by weight of heat-treated dextrin and confirming that the pH was 5.8. Then, the temperature of the solution was raised to 95 ° C. and used in Example 1 herein. Alpha-amylase was added 0.1% by weight relative to the heat treated dextrin and allowed to react for 2 hours. To stop the action of the enzyme, lower the pH to 3.0 or less with 1N hydrochloric acid solution, cool the solution to 55 ° C, adjust the pH to 5.5 with 1N sodium hydroxide solution, and then adjust the pH to 5.5 (Genencor International, BBA 1500, FINLAND). ) Was added to the dextrin by 0.1% by weight, while transglucosidase (Amano Pharmaceutical Co., Ltd., JAPAN) was added by 0.2% by weight to the dextrin and reacted for 48 hours.

1 wt% of powdered activated carbon was added to the enzyme reaction with dextrin, and the enzyme was inactivated by heating, filtered using a glass filter, decolorized and desalted through an ion exchange resin, and concentrated to a concentration of 50% by a vacuum concentrator. Ethanol was added to twice the weight of the solution, and then allowed to stand overnight at room temperature to separate the supernatant and the precipitate. Distilled water was added to the obtained precipitate to dissolve it, concentrated to 50% concentration to remove residual ethanol, and then spray-dried to have excellent whiteness, and more than 90% of digestive monosaccharides and disaccharides were removed to further increase the content of indigestible components. Fibers were prepared. The plant fiber obtained in this manner was 84, and the content of the indigestible component was found to be 90% by HPLC method.

Example 3

500 g of the purified 50% enzyme-treated sugar solution obtained in Example 2 was passed through a jacket column containing 41 Diion Ubikei-530 resin (Mitsubishi Kasei Technoengineers Ltd., JAPAN). At this time, the hot water was circulated to the pump through the jacket to adjust the temperature of the column to 60 ℃ and the flow rate was SV = 0.37. The plant fiber obtained in this way was 85 and the content of indigestible components was 95%.

Example 4

The white dextrin obtained in Example 1 was placed in a kneader, and 10% by weight of gum arabic was added to the dextrin and mixed uniformly for 30 minutes. Alpha-amylase was applied in the same manner as in Example 1, the pH was lowered to inactivate the enzyme, the temperature was adjusted to 55 ° C. and the pH was readjusted to 4.5. To the dextrin, 0.2% by weight of glucoamylase (Novo Nordisk Bioindustry Ltd., AMG 300L, DENMARK) was added and allowed to react for 36 hours. Got it. The precipitate was formed by adding 95% ethanol, which is twice the weight of the sugar solution, and then allowed to stand at room temperature for 2 hours to remove the supernatant by centrifugation. Then, distilled water was added to the precipitate and concentrated to 50% by a vacuum concentrator. The residual ethanol was removed and then spray dried to prepare plant fibers having excellent whiteness and increased content of indigestible components. The plant fiber obtained in this way was 85 and the content of indigestible components was 93%.

Example 5

500 g of a 50% concentration of sugar solution treated with glucoamylase obtained in Example 4 was passed through a column containing Diion Ubikei-530 resin. At this time, the temperature of the column was maintained at 60 ° C. and the flow rate was SV = 0.4. Thus obtained fire-retardant polymer material was concentrated to 50% concentration, and dried through a spray dryer to produce a plant fiber having excellent whiteness and a high content of the indigestible component. The plant fiber had a whiteness of 83 and the content of indigestible components was 90%.

Example 6

Alpha-amylase and glucoamylase were applied to the mixture of white dextrin and gum arabic obtained in Example 4, followed by inactivation of the enzyme and transglucosidase (Amano Pharmaceutical Co., Ltd., JAPAN) 0.2 to dextrin. By weight percent was added and reacted for 48 hours at pH 5.5 and a temperature of 55 ° C. After the activated carbon treatment and general sugar refining process, and concentrated to 50% concentration, in the same manner as in Example 4 was added to ethanol corresponding to twice the weight of the sugar solution and powdered. The plant fiber obtained in this way was 85 and the content of the indigestible component was 97%.

Example 7

1000 g of the 50% enzyme-treated sugar solution obtained in Example 6 was passed through a column filled with 81 DIION UBI-530 resins. In the same manner as in Example 5, the conditions of the column were maintained and partitioned, and the obtained plant fibers had a whiteness of 83 and an indigestible component content of 95%. For reference, the whiteness of polydextrose, a synthetic plant fiber made by condensation polymerization of glucose, sorbitol, and citric acid was 75, and the content of indigestible components was 67%.

Example 8

Confirmation of Intestinal Dress Effect

Five dogs were housed in a group of individual cages in a small animal breeding room with an initial weight of 50g and the Sprague-Dawley system was adjusted to 23 ± 2 ° C. Basic feed having the ingredients and composition as shown in the addition of 5% (v / v) of the plant fiber prepared in Examples 1 to 7 to the basic feed, 5% (v / v) of cellulose to the basic feed Each was bred for 7 days. Water and feed were taken freely, and feed intake and weight changes were recorded daily. On the 7th day, carmine (pigment) was mixed and administered to the feed, and the time until carmine appeared in the feces was measured, and this was made into excretion time. The slaughter was then slaughtered to remove the cecum, and the weight of the cecum, the pH of the contents and the amount of butyric acid were measured. The average obtained above is shown in Table 3 below.

As shown in Table 3, the plant fibers prepared in Examples 1 to 7 reached the large intestine in an ovarian state, and was metabolized into organic acids by the activity of intestinal bacteria to lower the pH of the intestine. Excretion time was shortened in any of the intakes of the plant fibers prepared from Examples 1 to 7, but it was confirmed that the intake of the plant fibers prepared from Examples 3 and 6 was effective compared to the cellulose intake group in which the variation improvement effect was apparent. . Therefore, in the following examples, the clinical experiments were conducted on the plant fibers prepared in Example 6 to confirm the effects.

Example 9

[Identification of Serum Cholesterol and Triglyceride Lowering Effect]

The serum lipid-improving effect of the plant fibers prepared from Examples 1 to 7 was examined: Sprague-Dawley rats having an initial body weight of 50 g were fed with a high-sugar diet (basic feed) shown in Table 2 after 5 weeks of pre-breeding. 10 animals per group), the first group (adjusted group) basic feed, the second group (Example 1), the third group (Example 2), the fourth group (Example 3), the fifth group (Example 4), Group 6 (Example 5), Group 7 (Example 6), and Group 8 (Example 7) were supplied with a test feed containing 5 parts of feed each added to 95 parts of basic feed. Breeding for 9 weeks. At this time, the feed and beverages were freely ingested, and after 4 hours of fasting at 9 weeks, blood was collected and the serum total cholesterol and triglyceride levels were measured by enzyme method, and the results obtained are shown in Table 4 below.

The results were all averaged for each test group, and feed efficiency was calculated by the following equation.

As shown in Table 4, there was no difference in weight gain and feed efficiency among the eight groups. On the other hand, the serum total cholesterol and triglyceride values of rats fed with feed containing plant fiber prepared in Examples 1 to 7 were confirmed to be clearly lower than the control group, respectively. In particular, it was proved that the effect of Example 3 and Example 6 was remarkable.

Example 10

Confirmation of blood glucose level and insulin secretion effect

In the experimental results using 36 rats, the fasting blood sugar level was 78.0 mg / dl, and the average insulin secretion was 17.3 μU / ml. However, 1.5 g / kg of body weight was orally administered with sugar, and the blood glucose level and insulin secretion were increased. After minutes, peaks were reached at an average of 164 mg / dl and 49.9 μU / ml, and after 120 minutes, they returned to normal. However, when the sugar intake of the plant fiber prepared from Example 6 of 0.15-1.5 g (1 / 10-1 / 1 of sugar) in 1/10 ratio of the above-mentioned sugar, the blood glucose level after 30 minutes averaged 144 mg / dl As a result, insulin secretion was 32.3 µU / ml on average and 138 mg / dl and 31.1 µU / ml when ingested at a 1/1 ratio.

As a result, it has been found that the increase in blood sugar level and insulin secretion by sugar is suppressed by a significant difference by the indigestible dextrin. In the same manner, the blood glucose level after 30 minutes when 0.15 g was added to the above-described sugar in the plant fiber prepared in Example 6 was found to be no difference from that of orally administered sugar.

As described and demonstrated in detail above, the present invention prepares a heat treatment dextrin (excellent whiteness) using a starch material, and then mixed with a fire-resistant food polymer material dry, the whiteness and indigestible components It provides a method for producing a high content of plant fibers. In addition, the plant fiber produced by the present invention is somewhat more advantageous in the white cotton than the plant fiber material made of underground starch, in particular potato starch material, reaches the large intestine in the state of ovarian function, and the cholesterol and triglyceride level in serum Since it has been found to be effective in lowering blood sugar and increasing insulin secretion, it is expected that the field of foods that can be applied through this will be further expanded.

Claims (6)

(i) pre-drying the raw starch to a temperature of 80 to 100 ℃ to maintain moisture at a level of 4 to 7%, and then to the starch is added 0.04 to 0.1% by weight of gaseous hydrochloric acid with high temperature hot air compared to starch, 110 Heat treatment at a temperature of from about 180 ° C. for 60 to 90 minutes; (ii) mixing the neutralizing agent in a dry state to adjust the pH to 5.5 to 6.5, and fractionating to obtain a heat treated white dextrin; And (iii) adding 1 to 20% by weight of an indigestible functional polymer to the electrothermally treated dextrin and mixing it with 0.05% by weight of the heat-resistant alpha-amylase, and performing filtration, decolorization and desalting. Method for producing a starch material plant fiber with a high content of whiteness and indigestible ingredients. The method according to claim 1, wherein the neutralizing agent is ammonium bicarbonate or anhydrous sodium bisulfite. The method according to claim 1, wherein the heat treated dextrin has a fluidity of 50 to 60 ml, a soluble substance of 86% isomerized, a starch saccharification of 4 to 7 and an indigestible component content of 35 to 50%. A method for producing starch material plant fiber having a high content of whiteness and indigestible component, characterized in that. The method of claim 1, wherein the heat-resistant alpha-amylase prepared in a starch material plant fiber with a high content of whiteness and indigestible components, characterized in that directly added to the mixed solution of the heat-treated dextrin and functional polymer adjusted to pH 5.5 to 6.5 Way. The method of claim 1, further comprising, after the heat-resistant alpha-amylase action, reacting a mixture of beta-amylase and transglucosidase or a mixture of glucoamylase and transglucosidase at 0.05 to 0.5% by weight relative to dextrin, respectively. Method for producing a starch material plant fiber high in whiteness and indigestible components, characterized in that. The sugar solution according to claim 1, wherein the sugar solution, which has been filtered, decolorized, and desalted after the enzymatic reaction of the heat resistant alpha-amylase, is concentrated to a concentration of 50%, and then passed through an ion exchange resin column or ethanol is added to precipitate the precipitate. A method of producing starch material plant fiber having a high content of whiteness and indigestible component, further comprising the step of separating at least 95% of the monosaccharides from the solution by standing at room temperature.