JPWO2020159658A5

JPWO2020159658A5 -

Info

Publication number: JPWO2020159658A5
Application number: JP2021543454A
Authority: JP
Publication date: 2024-02-08
Anticipated expiration: 2039-12-31

Description

ピペットチップは、さらにピペットチップの内側に沿って配置されたピペットプランジャを有し、ピペットプランジャは、プランジャ上部、プランジャセンタリング部、遠位先端部を有するプランジャチップを有する。１つの実施形態において、境界部のレセプタクルは、プランジャセンタリング部を受け入れるように構成され、流体チャネルは、プランジャチップの遠位先端部を受け入れるように構成される。 The pipette tip further has a pipette plunger disposed along the interior of the pipette tip, the pipette plunger having a plunger tip having a plunger top, a plunger centering portion, and a distal tip. In one embodiment, the interface receptacle is configured to receive the plunger centering portion and the fluid channel is configured to receive the distal tip of the plunger tip.

液体処理システムは、さらにピペットチップの内側に沿って配置されたピペットプランジャであって、ピペットプランジャは、プランジャ上部、プランジャセンタリング部、及び遠位先端部を有するプランジャチップを有し、レセプタクルは、プランジャセンタリング部を受け入れるように構成され、流体チャネルは、プランジャチップの遠位先端部を受け入れるように構成されたピペットプランジャを含む。 The liquid handling system further includes a pipette plunger disposed along the interior of the pipette tip, the pipette plunger having a plunger tip having a plunger top, a plunger centering portion, and a distal tip, and the receptacle having a plunger tip disposed along the inside of the pipette tip. The fluidic channel includes a pipette plunger configured to receive the distal tip of the plunger tip.

ピペットプランジャ１３０は、ピペットチップ１０５の内側に沿って配置される。ピペットプランジャ１３０は、プランジャ上部１３２、プランジャセンタリング部１３４、及び遠位先端部１３８を含むプランジャチップ１３６を含む。プランジャ上部１３２は、例えば、容積式ピペットチップ１００を用いて液体を吸引及び／または分配する液体処理機器（図示せず）の接続部品（図１５Ａから図１６Ｂ参照）に適合される。さらに、プランジャセンタリング部１３４は、ピペットチップ１０５の境界部１１０のプランジャストップレセプタクル（またはキャビティ）１２２に適合され、ここでプランジャストップレセプタクル（またはキャビティ）１２２は、ピペットプランジャ１３０のプランジャセンタリング部１３４を受け入れるように設計される。プランジャセンタリング部１３４は、ピペットプランジャ１３０の「停止」機構の機能を果たす。プランジャチップ１３６とピペットプランジャ１３０の遠位先端部１３８は、ピペットチップ１０５のピペットチップ本体１１２の流体チャネル１１４に適合される。さらに、プランジャチップ１３６の遠位先端部１３８は、ピペットチップ１０５の流体チャネル１１４の遠位端において開口１１６と先細りしたチップ１１８に接続及び封止するように設計される。ピペットプランジャ１３０の例のさらなる詳細は、図７Ａから図９Ｂ及び図２０を参照して以下に示され記載される。 Pipette plunger 130 is positioned along the inside of pipette tip 105 . Pipette plunger 130 includes a plunger tip 136 that includes a plunger top 132, a plunger centering portion 134, and a distal tip 138. The plunger top 132 is adapted, for example, to a connecting part (see FIGS. 15A to 16B) of a liquid handling device (not shown) that aspirates and/or dispenses liquid using the positive displacement pipette tip 100. Additionally, the plunger centering portion 134 is adapted to a plunger stop receptacle (or cavity) 122 of the border 110 of the pipette tip 105, where the plunger stop receptacle (or cavity) 122 receives the plunger centering portion 134 of the pipette plunger 130. Designed to be. Plunger centering portion 134 serves as a “stop” mechanism for pipette plunger 130 . Plunger tip 136 and distal tip 138 of pipette plunger 130 are fitted into fluid channel 114 of pipette tip body 112 of pipette tip 105 . Additionally, the distal tip 138 of the plunger tip 136 is designed to connect and seal to the aperture 116 and the tapered tip 118 at the distal end of the fluid channel 114 of the pipette tip 105. Further details of example pipette plungers 130 are shown and described below with reference to FIGS. 7A-9B and 20.

Claims

An automatic positive displacement pipette tip,
A pipette tip having a border and a pipette tip body, the border having an inner wall and a receptacle, the pipette tip body having a fluid channel along the length of the pipette tip body, the pipette tip body having a fluid channel along the length of the pipette tip body, a pipette tip, the channel having an opening at a distal end of the fluidic channel;
a pipette plunger disposed along the inside of the pipette tip, the pipette plunger having a plunger tip having a plunger top, a centering portion, and a distal tip;
the receptacle is configured to receive the centering portion to function as a stop mechanism for the pipette plunger;
the fluid channel is configured to receive the distal tip of the plunger tip;
the plunger top has an outer wall including a vertical outer wall feature;
The pipette tip , wherein the plunger top is attached to a connecting part of a liquid handling device by the vertical outer wall mechanism .

The pipette tip of claim 1, wherein the fluid channel has a tapered tip at a distal end of the fluid channel.

3. The pipette tip of claim 2, wherein the distal tip of the pipette plunger has a rounded portion configured to connect with the aperture and the tapered tip at the distal end of the fluid channel. .

4. The pipette tip of claim 3, wherein the distal tip has a tapered sidewall that corresponds to the tapered tip of the fluid channel.

the fluid channel has walls;
The distal tip is slidable against and along a wall of the fluid channel sufficient to minimize the space between the tapered tip of the fluid channel and the tapered sidewall of the distal tip. 5. The pipette tip of claim 4, having an outer top end dimensioned to seal.

6. The pipette tip of claim 5, wherein the outer upper end has a sealing ring feature formed therein.

The pipette tip of claim 1, wherein the pipette tip is formed of a polymeric material.

8. The pipette tip of claim 7, wherein the pipette plunger is formed of the same polymeric material as the pipette tip.

8. The pipette tip of claim 7, wherein the pipette plunger is formed of a different polymeric material than the polymeric material of the pipette tip.

The pipette tip of claim 1, wherein the inner wall of the boundary portion includes a vertical inner wall feature.

11. The pipette tip of claim 10, wherein each of the vertical inner wall features is a ridge or a protruding vertical line formed along the inner wall of the boundary.

The plunger upper part has an outer wall,
The pipette tip of claim 1, wherein the outer wall includes a vertical outer wall feature.

13. The pipette tip of claim 12, wherein each of the vertical outer wall features is a ridge or a protruding vertical line formed along the outer wall of the plunger top.

2. The pipette tip of claim 1, wherein the border has an upper rim with a set of spacer features.

A pipette tip having a border and a pipette tip body, the border having an inner wall and a receptacle, the pipette tip body having a fluid channel along the length of the pipette tip body, the pipette tip body having a fluid channel along the length of the pipette tip body, a pipette tip, a channel having an opening at a distal end of the fluidic channel;
a pipette plunger disposed along the inside of the pipette tip, the pipette plunger comprising:
The pipette plunger has a plunger tip having a plunger top, a plunger centering portion, and a distal tip;
the receptacle is configured to receive the plunger centering portion;
the plunger upper part has an outer wall with a vertical outer wall mechanism;
The upper part of the plunger is attached to a connecting part of a liquid treatment device,
a pipette plunger, the fluid channel configured to receive the distal tip of the plunger tip;
a pipette tip gripping mechanism configured to manipulate the pipette tip and the pipette plunger ;
The pipette tip gripping mechanism includes a pipette tip collet and a pipette tip fixing mechanism,
The pipette tip collet includes a pipette tip collet upper ring plate and a collet chuck part for attaching to the inside of the boundary part of the pipette tip.
liquid handling system.

16. The liquid handling system of claim 15, wherein the collet chuck portion has a serrated feature configured to increase friction of the pipette tip against the interface.

17. The liquid handling system of claim 16, wherein the collet chuck portion is a segmented band or sleeve for fitting inside the boundary.

18. The liquid handling system of claim 17, wherein the collet chuck portion is configured to expand and secure to the inner wall of the boundary portion.

19. The liquid processing system of claim 18, wherein the pipette tip fixing mechanism includes a pipette tip fixing upper ring plate and a hollow sleeve part for fitting inside the collet chuck part.

the collet chuck portion is configured to expand against an inner wall of the interface when the hollow sleeve portion is fitted inside the collet chuck portion of the pipette tip collet;
20. The liquid of claim 19, wherein the collet chuck section is configured to recede and loosen relative to the interface when the hollow sleeve section is withdrawn from the collet chuck section of the pipette tip collet. processing system.

16. The liquid handling system of claim 15, wherein the zero insertion force pipette tip gripping mechanism includes a pipette plunger collet and a pipette plunger securing mechanism.

22. The liquid handling system of claim 21, wherein the pipette plunger collet has a shaft portion and a collet chuck portion for fitting around a plunger top of the pipette plunger.

the collet chuck portion is a segmented band or sleeve for fitting around the plunger top of the pipette plunger;
23. The liquid handling system of claim 22, wherein the collet chuck section is configured to expand and lock against an outer wall of the plunger top inside the collet chuck section.

24. The liquid handling system of claim 23, wherein the pipette plunger securing mechanism includes an upper ring plate and a hollow sleeve portion that fits around the pipette plunger collet.

The collet chuck portion is configured to close against the plunger top of the pipette plunger when the hollow sleeve portion slides around the collet chuck portion of the plunger collet, and the collet chuck portion is configured to close against the plunger top of the pipette plunger when the hollow sleeve portion slides around the collet chuck portion of the plunger collet. 25. The liquid handling system of claim 24, configured to release the plunger top of the pipette plunger when the collet chuck section is withdrawn from the collet chuck section of the plunger collet.

The zero insertion force pipette gripping mechanism includes a pair of pipette tip plunger gripping mechanisms, a pair of pipette plunger collets, a fixed upper plate, a lower plate, and two springs between the upper plate and the lower plate;
an upper end of the pipette plunger collet is fixed to the upper plate;
the lower plate and the two springs are movable relative to the upper plate;
each spring is configured to provide a spring force to a corresponding pipette plunger securing mechanism through the lower plate in a slidable manner;
16. The liquid handling system of claim 15, wherein the pipette plunger locking mechanism is movable relative to the pipette plunger collet.