JPS6260560A - Plasma sampling apparatus - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] <Application fields on aS> The present invention separates plasma from blood collected from a blood donor.

This invention relates to a membrane molecule type 11 plasma collection device.

<Prior art> Plasma fractionation agents (purified by plasma) are widely used for various treatments. Most of them are imported. Blood collection by towing method , - blood sampling m is 20
The limit is 0 ml.

According to a method that collects only plasma and returns blood cell components to the donor, even if twice the current amount is collected, there will be no effect on the human body. Therefore, consideration is being given to collecting plasma using such a method and increasing the domestic plasma self-sufficiency rate.

The conventional method for separating plasma is to add an anticoagulant (hereinafter referred to as ACD solution) to blood collected from a donor, and then centrifuge the blood to separate the plasma. When such a centrifugal separation method is used, there are drawbacks that the equipment is expensive and the serum sampling operation takes time.

Recently, a minute Ml was developed that uses hollow fibers with fine holes in a straw-shaped polymer membrane with a hollow inside.
When a membrane is used, the plasma collection device can be constructed at low cost, and the time required for plasma collection can be shortened.

However, even with such a plasma collection device using a membrane module, the priming operation that is performed prior to the operating operation is performed manually, which increases the overall plasma collection time and results in the donor being restrained for a long time. It had become.

<Problems to be Solved by the Invention> A technical problem to be solved by the present invention is to enable the priming operation to be performed efficiently in the membrane separation type extraction 572 apparatus.

Means for Solving the Problems> The configuration of the present invention consists of a blood circuit connected to a needle inserted into a blood donor, a blood pump that transports blood in this blood circuit, and a membrane that connects the blood to two chambers, the outside and the outside. a separator which separates plasma in the blood by the pressure gradient of the two chambers and the permeation effect of the membrane; a blood bag connected to the separator; a drainage bag; a plasma bag for storing the plasma separated by the separator; a means for returning the blood in the blood bag to the donor each time the weight of the blood bag reaches a predetermined value; and a saline bag for storing physiological saline connected to a branch provided in the circuit, and prior to operation, the branch is connected to the downstream side of the separator with the drain bag connected to the downstream side of the separator. The blood circuit and the separator are opened and physiological saline is introduced and filled into the blood circuit and the separator.

Function> The above technical means works as follows. That is, prior to the operation, with the drainage bag connected to the downstream side of the separator, a clamp provided on the branch is opened, and physiological saline is introduced into the blood circuit and the separator by the blood pump. do.

After the priming operation for these parts is completed, a pump that forms a pressure gradient between the two internal and external chambers of the hibarator is repeatedly driven in both forward and reverse directions, thereby alternately reversing the pressure gradient between these chambers. The blood circuit is cleaned.

Note that the physiological saline used for washing is then expelled by newly introduced physiological saline and by blood supplied from the donor by puncturing the membrane into the blood donor, and drained into the drainage bag.

<Example> The present invention will be described in detail below with reference to the drawings. The figure is a configuration diagram of an apparatus according to an embodiment of the present invention. In the figure, A is a blood donor, B is a first blood circuit through which blood from the donor flows, C is a second blood circuit that circulates through the inner chamber of the separator and the blood bag 3, which will be described later, and 1 is a blood circuit in which blood from the donor flows. The ACD liquid bags 2a and 2b storing ACD liquid are saline bags storing physiological saline, and the rawhide bag 2a is connected to a branch provided in the second blood circuit C. 3 is a blood bag for storing collected blood, 4 is a plasma bag for storing plasma separated from the collected blood, 5 is a drainage bag for storing drainage fluid, and 68 to 6f are each fluid (ACD liquid, etc.). physiological saline, etc.)
The first to sixth detectors 7a to 7e are pumps for supplying ACD liquid, which supply fluid (blood, air, etc.);
The first blood pump, the second blood pump, the air pump, and the serum sampling pump, the first blood pump 7b and the air pump 7.
d can be driven in both forward and reverse directions.

88 to 89 are l 11 of each flow path through which each of the fluids flows.
The first to seventh clamps, 9a to 9d, are 0'! 1~
A fourth pressure gauge, 10a to 10C are first to third chambers, 11a to 11c are blood detectors, 12a to 12C are first to third needles, and 13 is, for example, a hollow straw-shaped height gauge. This is a separator made by bundling a large number of hollow fibers with fine holes in the molecular membrane and placing them in a cylindrical container.The inside of each hollow fiber forms an inner chamber, and the outside and form. In addition, in order to facilitate understanding, this part is schematically represented by one hollow fiber in the figure. OT2 blood circuit C
is connected to this inner chamber. 14 is a weight detector for detecting the type of blood bag 3.

A part E surrounded by a dashed line is a control part, which includes f
Signal from f1fft detector 14, blood detector 118~
11c or the first to fourth pressure gauges 9a to 9d
Time-divisionally capture signals from /L, Chibrexa (
MPX) 15a, a Δ/D converter 15b that converts the analog signal from the MP , a read-only memory (ROM) 15e1 in which processing routines, arithmetic processing programs, etc. are stored, converts digital signals from the CPU 15c to D'/A, and supplies the ACD liquid supply pump 7a, first and second blood pumps 7b, 7c1. It includes a D/A converter 15f that outputs a drive signal (the number in parentheses corresponds to the code of the part to which the output is given) to the air pump 7d and the serum sampling pump 7e, and each of these except the MPX 15a The elements are connected to each other via a bus 15g.

Next, the operation of the neck brace according to the embodiment of the present invention constructed in this way will be explained. The briming operation is performed as follows. The first, third and fourth clamps 8c and 8d are in between and the remaining clamp 8a.

With needles 8b, 8e, 8f, and 8g in the leaping state, the second and third needles 12b and 12c are inserted into the saline bags 2a and 2b, respectively, and the air pump 7d is driven clockwise or in the forward direction to insert the blood bag. 3 and the air present in the flow path from this bag to the air pump 7d is sucked. And the third
When the pressure gauge 9C shows a predetermined positive pressure (-PmmHq), the air pump 7d is stopped.

Incidentally, ff1ffi of the blood bag 3 at this time is detected by the superposition detector 14 and inputted to the control section E as a signal representing the tare weight of the blood bag 3. The upper and lower limit values of the blood bag 3 are zero-corrected using this tare mm signal, and in the operating operation, blood is returned and blood is collected based on the upper and lower limit set values.

After this, the second clamp 8b and the fifth clamp 8e.

The sixth clamp 8f and the seventh clamp 89 are interposed, and the second blood pump 7C is driven. As a result, with the drainage bag 5 connected to the downstream side of the separator 13, physiological saline is introduced into the second blood circuit C from the saline bag 2a via the branch, and the air present in this part is removed. be done.

After this, when the third detector (physiological saline detector) 6c starts operating and a certain period of time (usually 1-2 seconds) has elapsed, the second detector 6c starts operating.
Clamp 8b is opened.

Next, the first clamp 8a is opened, and the ACD liquid supply pump 7a and the first blood pump 7b are driven. This ACD liquid supply pump 7a is driven to remove air present in the flow path through which the ACD liquid flows, and for a certain period of time after the first detector (ACD liquid detector) 6a starts operating. After the elapse of (usually 1 to 2 seconds), the ACDI supply pump 7a is stopped.

Thereafter, the fourth detection lever 6d detects the liquid level at the inlet of the separator 13, and after a certain period of time (usually several seconds) has elapsed since this liquid level detection, the sixth clamp 8f is closed and the second chamber (separator inlet chamber) is closed. The liquid level at 10b is determined. Similarly, the outlet liquid level of the separator 13 is detected by the fifth detector 6C, and after a certain period of time (usually several seconds) has elapsed since this liquid level detection, the seventh clamp 89 is closed and the third The liquid level of the chamber (separator outlet chamber > 10c) is determined.

Raw food bag 2a to raw food 1! ! ! When the saline solution flows, the air pump 7d is driven clockwise or in the forward direction,
A positive pressure is applied to the outside of the separation membrane of the separator 13, and physiological saline is introduced to the outside of the separation membrane. This part is raw J! l! The sixth detector 6f detects that it is filled with fR salt water, and drives the air pump 7d in the opposite direction (counterclockwise). By driving the air pump 7d in this manner, a positive pressure is applied to the outside of the separation membrane of the separator 13, and physiological saline is guided to the inside of the atm membrane through the membrane of the separator 13. By repeatedly driving the air pump 7d in both forward and reverse directions, the blood circuit is cleaned using physiological saline.

After this, an operating operation is performed. That is, the third part 12c that had been punctured in the saline bag 2b was pulled out and the blood donor △
stung on the arm, etc. Further, the first lamp 8a is opened, and the ACD liquid supply pump 7a and the second blood pump 7C
is driven, and the first blood pump 7b is driven in the forward direction.

The physiological saline that has been flowing into the blood circuit due to the priming operation is displaced by the blood supplied from donor A through the third needle 12G, and is finally discharged into the drainage bag 5. . When blood is detected by the blood detector 11b, the fifth clamp 8e is closed, and the fourth clamp 8d is closed.
is opened.

Therefore, the blood collected from donor △ is transferred to blood bag 3.
is stored in When blood starts to accumulate in the blood bag 3, the total weight of the blood bag 3 gradually increases from the tare of the blood bag, and when the total weight reaches the limit set value, the ACD liquid supply The pump 7a is stopped and the first blood pump 7b is driven in the opposite direction to return blood. Incidentally, the second blood pump 7C is continuously driven at the same time as the start of the operation operation,
The serum sampling pump 7e is continuously driven from the time when the separator 13 becomes accustomed to the blood.

Due to the blood return, the total weight of the blood bag 3 decreases, and when it reaches the lower limit, the first blood pump 7b is switched and driven in the forward direction, and the ACD liquid supply pump 7a is driven again. Such blood collection and blood return are repeated 11 until the target blood collection and blood injection are performed.

When the target plasma collection ■ is reached, the blood collection operation begins. That is, the first blood pump 7b is driven in the opposite direction, the second clamp 8b is opened, and after a predetermined time 1iil has elapsed, the third clamp 8G is opened.

The second blood pump 7C continues to be driven in the same direction and saline flows into the second blood circuit C via a branch. As a result, the blood accumulated in the second blood circuit C is pushed out, and blood components such as blood cells adhering to the inner wall surface of this blood circuit are also washed out at the same time. Such operations are similarly performed in the separator 13, blood bag 3, and blood circuit B connected to the second blood circuit C, and the blood in these parts is collected and returned to the blood donor A.

The end of blood return is detected by the blood detector 11a placed in front of the donor Δ in the blood circuit B, and when the blood concentration falls below a predetermined value, the first blood pump 7b and the second blood pump 7C is stopped and the first clamp 8a is opened.

<Effects of the Invention> According to the present invention, since the priming operation is performed completely automatically based on the signal from the control unit E, the work efficiency is high. Since the pump 7d is driven in both forward and reverse directions for cleaning, the separator 13 portion, particularly the separation membrane portion, can be efficiently cleaned.

[Brief explanation of the drawing]

The figure is a configuration diagram of an apparatus according to an embodiment of the present invention. A... Blood donor, B... First blood circuit, C... Second blood circuit, D... Branch, E... Control unit, 2a,
2b... Saline bag, 3... Blood bag, 4...
Plasma bag, 7b...first blood pump, 7C...
Second blood pump, 7e... Serum collection pump, 8a to 8g
...Clamp, 9a-9d...Pressure gauge, 118-1
1c...Blood detector, 12a-12G...Button, 13
...Separator, 14...Weight detector

Claims (1)

[Claims] A blood circuit connected to a needle inserted into a blood donor, a blood pump for transporting blood in this blood circuit, and a membrane divided into two chambers, an inner and outer chamber, the blood circuit is connected to one of these chambers, and the blood circuit is connected to one of these chambers. a separator that separates plasma in blood by a pressure gradient in two chambers and a permeation effect of the membrane; a blood bag and a drainage bag that are switchably connected to the separator;
a plasma bag for storing plasma separated by the separator; a means for returning blood in the blood bag to the donor each time the weight of the blood bag reaches a predetermined value; A saline bag for storing physiological saline is connected to the branch, and prior to the operation,
A blood sampling device characterized in that the branch is opened with the drainage bag connected to the downstream side of the separator, and physiological saline is introduced and filled into the blood circuit and the separator.