JPS6078334A - Fluorophotometer - Google Patents

Abstract

PURPOSE:To decrease the scattered light quantity and to strengthen the strength of a sample cell even if the volume of said cell is decreased by providing the masking of opaque members at short sides of the sample cell having rectangular cross-section made of a transparent member and the opaque members. CONSTITUTION:The sample cell 10 is formed by sticking the transparent member (transparent quartz) 2 and the opaque members (opaque quartz) 3A-3C so that a horizontal section of a sample part 100 for feeding the sample becomes rectangular. The cell 10 is formed by masking the other parts of the short sides with opaque members 3A, 3B so that a short side light entrance surface 120 of the part 100 becomes the same as a length of the short side. By such a formation, the scattered light quantity is decreased and the strength of the sample cell can be strengthened even if the volume of the sample cell is decreased.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a fluorometer, and particularly to a fluorometer suitable for extremely reducing sample cell capacity.

[Background of the invention]

Traditionally, this type of fluorescence needle has been equipped with a light source that emits excitation light of a single wavelength, an excitation spectrometer that irradiates a sample cell with the excitation light from the light source, and an excitation light that emits fluorescence emitted from the sample in the sample cell. The structure generally includes a fluorescence spectrometer that measures the spectral value after the sample is taken out from the right angle to prevent it from entering.

Figures 1 (I) and 2 show a microcell for fluorescence measurement used in the above-mentioned fluorometer. ) is a sectional view taken along the line ■-■. The structure of the micro cell for fluorescence measurement shown in these figures is explained in detail in Japanese Utility Model Application No. 51-Q327, so the structure will be briefly described here.

In FIG. 1 CI) and (ID), the cell 1 uses transparent quartz 20 as the transparent member 2, black quartz 30 as the opaque member 3, and the black quartz 30 is placed at the four corners of the transparent quartz 20. The black quartz 30 is bonded to have a masking effect.In other words, the masking effect of the black quartz 30 is to prevent the light scattered on the wall surface of the cell 1 from going toward the fluorescence spectrometer. There is.

By the way, in order to maintain the adhesive strength between the opaque member 3 and the transparent part 2, it is natural that the wall of the sample cell 1 needs to have a certain thickness or more. However, in the above-mentioned prior art, when the capacity of the sample cell 1 is made extremely small, the loss of light quantity becomes extremely large due to the relatively large size of the opaque member 3 and its thickness. This is a fatal flaw, especially in a fluorometer for liquid chromatography where the sample cell 1 is required to withstand pressure.

[Purpose of the invention]

An object of the present invention is to eliminate the drawbacks of the prior art described above, reduce the amount of scattered light even if the capacity of the sample cell is reduced, and
An object of the present invention is to provide a fluorometer with enhanced sample cell strength.

[Summary of the invention]

In order to achieve the above object, the present invention is configured by fixing a transparent member and an opaque member so that the cross section of the sample section of the sample cell into which the sample is placed is rectangular. The other part of the short side is masked with an opaque member so that the length of the short side is at least equal to or longer than the length of the short side.

Further, the present invention is characterized in that the luminous flux of light passing through the entrance/exit surface of the short side of the sample section of the sample cell is made smaller than the luminous flux of light passing through the long side of the sample section. .

[Embodiments of the invention]

Hereinafter, preferred embodiments of the present invention will be described based on the drawings.

FIG. 2 is a configuration diagram showing an embodiment of a fluorometer according to the present invention. Moreover, FIG. 3 is a sectional view showing the structure of a sample cell used in this example.

In these figures, the fluorometer includes a light source 4 that emits excitation light of a single wavelength, and a sample cell in which the excitation light from the light source 4 is configured such that the sample section 100 into which the sample is placed is rectangular in horizontal cross section. An excitation spectrometer 5 that irradiates the long side 110 of the sample section 100 of 10, and an entrance/exit at the end of the short side of the sample section 100 provided at right angles to prevent excitation light from entering the fluorescence emitted from the sample in the sample cell 10. It is configured to include a fluorescence spectrometer 6 that measures the spectrum after being taken out from the surface 120.

Further, to explain the structure of the sample cell 10 in more detail, the sample cell 10 has a transparent member (transparent quartz) 2 and an opaque member (opaque quartz) 3 fixed to each other, and the horizontal cross section of the sample section 100 into which the sample is placed is rectangular. In addition, the sample section 10
The other part of the short side is covered with the opaque member 3 so that the short side front entrance/exit surface 120 of 0 is at least the same length as the short side.
It was constructed by masking.

Next, the structures of the excitation spectrometer 5 and the fluorescence spectrometer 6 will be explained.

The excitation spectrometer 5 has various parts arranged so that the excitation light emitted from the light source 4 is focused by a lens 51 and irradiated onto a mirror 53 through an entrance slit 52, and the excitation light reflected by the mirror 53 is collected. Each component is arranged so that the light is focused by a lens 57 via a concave diffraction grating 54, a mirror 55, and an output slit 56, and then irradiated onto the sample cell 10.

The fluorescence spectrometer 6 is arranged so that the sample cell 10 can extract the fluorescence emitted from the sample inside the sample cell 10 from the short side front entrance/exit surface 120 of the sample section 100, and includes a lens arranged on the short side front entrance/exit surface 120 of the sample section 100. Each component is arranged so that the fluorescent light can be condensed by a slit 61 and irradiated onto a concave diffraction grating 63 through a slit 62, and a slit 64 arranged in the direction of reflection of the concave diffraction grating 63, The detector 65 is configured to be able to detect fluorescence incident through the detector 65.

The operation of the fluorometer configured as described above will be explained below.

In FIG. 2, the excitation light emitted from the light source 4 enters the excitation spectrometer 5, is focused by the lens 51, and passes through the entrance slit 52, the mirror 53, the concave diffraction grating 54, the mirror 55, and the exit slit 56. Spectral spectroscopy. This separated light is focused near the center of the sample cell 10 by the lens 57. Fluorescence emitted by the sample in the sample cell 10 enters the fluorescence spectrometer 6 and passes through the lens 61 in the fluorescence spectrometer 6.
The light is focused by the entrance slit 62 and the concave diffraction grating 6.
3. The light is separated by the exit slit 64 and enters the detector 65. In order to prevent deterioration of the diffraction grating surface, the excitation spectrometer 5
Although the focal length and diffraction grating area are large, the fluorescence spectrometer 63 minimizes these as much as possible. As a result, even if the lattice constants of the diffraction gratings (54 and 63) used in the excitation spectrometer 5 and the fluorescence spectrometer 6 are the same, the line dispersion on the excitation side is larger than the line dispersion on the fluorescence side.

Therefore, in the same bandpass, the excitation spectrometer 5
The excitation spectrometer 5 has a wider slit width than the fluorescence spectrometer 6, and the width of the light beam at the center of the sample is also larger. In accordance with this, the shape of the sample cell 10 is such that the horizontal cross section of the sample section 100 is rectangular, as shown in FIG.

Since the long side is located at the excitation light incidence part and the short side is located at the fluorescence emission part, the cell capacity can be reduced to about 5 [μt] while minimizing light loss.

FIGS. 4 and 5 are cross-sectional views showing an embodiment in which the sample portion of the sample cell is further improved. In the sample cell shown in FIG. 3, in order to further reduce the cell capacity (5μ is expressed as 1 below), the sample portion 100 may be made smaller while keeping the outer size the same, or both the outer size and the dimensions of the sample portion 100 may be reduced.
J- In the former case, black member 3 must be thinned.
The thicknesses of A, 3B, and 3C are the dog, and the light amount loss is the dog in the convergent light and the diverging light. In the latter case, the adhesion between the black members 3A and 3B and the transparent member 2 is reduced, resulting in a decrease in cell strength.

In FIG. 4, only the sample portion 100 is made small, and the width of the transparent portion 2 on the fluorescence side is made larger than the short side of the sample portion 100, thereby preventing loss of the amount of light from the mold.

The actual image shown in Figure 3 has the advantage that the cell capacity can be reduced to 5 [μt] or less without compromising the cell frequency and keeping light loss low.
is superior to δ et al.

In FIG. 5, the same effect as in FIG. 4 is achieved by masking the fluorescence spectrometer 6, that is, by tapering the opaque portions 38 and 3B.

The sample cell 10 is an opaque member 3A whose short side light entrance/exit surface 120 of the sample section 100 masks that surface side.
and 3B are configured to have a taper 300 that widens outward from the portion in contact with the short side. Therefore, even when the excitation light is scattered by the inner wall (long side) of the cell, the scattered light is unlikely to enter the fluorescence spectrometer 6. In this respect, this embodiment is superior to the embodiment shown in FIG.

According to each embodiment of the present invention, in a fluorometer, 1)
There is an advantage that the cell capacity can be reduced from 5 μm to a very small amount without sacrificing the light utilization efficiency, 2) scattering light prevention effect, and 3) cell strength (withstand voltage).

Regarding the luminous fluxes of excitation light and fluorescence, in the above embodiment, the luminous flux on the excitation spectrometer 5 side was larger, but of course the reverse is not possible.

〔Effect of the invention〕

As described above, according to the present invention, the capacity of the sample cell can be miniaturized, and the strength of the sample cell can be strengthened.

[Brief explanation of the drawing]

FIG. 1 (I) is a front view showing the structure of a sample cell used in a conventional fluorometer, FIG. FIG. 3 is a cross-sectional view showing the structure of a sample cell used in the fluorometer according to the present invention, and FIGS. 4 and 5 are improved configurations of the same sample cell. 2... Transparent member, 3, 3A, 3B, 3C... Opaque member, 4... Light source, 5... Excitation spectrometer, 6... Fluorescence spectrometer, 10... Sample cell, 51... Lens, 5
2...Incidence slit, 53...Mirror, 54...
Concave diffraction grating, 55... Mirror, 56... Output slit, 57... Lens, 61... Lens, 62...
・Incidence slit, 63... Concave diffraction grating, 64...
Output slit, 65...detector, 100...sample section, 110...transparent member, 120...short side front entrance/exit surface. Figure 7 Figure 2 Figure 3 Figure ψ Figure 5 House

Claims (1)

[Claims] 1. A light source that emits excitation light of a single wavelength, an excitation spectrometer that irradiates a sample cell with the excitation light from the light source, and an excitation spectrometer that does not allow the fluorescence emitted from the sample in the sample cell to enter. In the fluorometer, the sample cell includes an opaque member and a transparent member that are fixed to each other, and a sample portion into which the sample is placed has a rectangular cross section. At the same time, other parts of the short side are masked with an opaque material so that the entrance/exit surface of the short side of the sample section is at least the same length as the short side. A fluorometer characterized by: 2. In claim 1, the sample cell is tapered such that the light entrance/exit surface on the short side of the sample section widens outward from the part where it contacts the short side with respect to the opaque member that masks that surface. A fluorometer characterized by comprising: 3. A light source that emits excitation light of a single wavelength, an excitation spectrometer that irradiates the sample cell with the excitation light from the light source, and extracts the fluorescence emitted from the sample in the sample cell from a direction where the excitation light does not enter. and a fluorescence spectrometer for measuring the spectrum of the sample cell, the sample cell comprising:
The opaque member and the transparent member are bonded together so that the sample part into which the sample is placed has a rectangular cross section, and the entrance/exit surface at the end of the short side is configured to be equal to or larger than the short side, and the center of the sample cell is A fluorometer, characterized in that either one of the excitation light flux and the fluorescence light flux is made smaller than the other and arranged on the entrance/exit surface side of the short side of the sample cell.