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JPS6485068A - Cell culture apparatus - Google Patents

Cell culture apparatus

Info

Publication number
JPS6485068A
JPS6485068A JP62239167A JP23916787A JPS6485068A JP S6485068 A JPS6485068 A JP S6485068A JP 62239167 A JP62239167 A JP 62239167A JP 23916787 A JP23916787 A JP 23916787A JP S6485068 A JPS6485068 A JP S6485068A
Authority
JP
Japan
Prior art keywords
liquid
molecular
culture
cell
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62239167A
Other languages
Japanese (ja)
Other versions
JPH0797982B2 (en
Inventor
Shoichi Kamimura
Hiroshi Takasugi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP62239167A priority Critical patent/JPH0797982B2/en
Publication of JPS6485068A publication Critical patent/JPS6485068A/en
Publication of JPH0797982B2 publication Critical patent/JPH0797982B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE:To carry out continuous culture of cell at high density, by circulating a culture liquid supplied to the cavity of a hollow fiber under pressure and circulating a culture liquid containing a low-molecular substance and a high- molecular valuable substance formed in a cell culture space through a membrane separation apparatus. CONSTITUTION:A culture liquid 5 is introduced into a hollow fiber 1 with a liquid pump 6, pressurized with pressure supplied from a pneumatic pressure applying apparatus 8 and circulated. A part of the circulating liquid is permeated into a cell culture space 4 to produce a low-molecular substance and a high- molecular valuable substance, which are temporarily stored in a chamber 19. The produced liquid 5a is introduced into a membrane separation apparatus 15 with a liquid pump 14 to separate low-molecular metabolic product such as lactic acid and ammonium ion with a separation membrane 16 and the treated liquid is circulated and concentrated. The high-molecular valuable substance is recovered at a definite flow rate with a liquid pump 18. The environment in the cell culture apparatus is maintained as uniformly as possible to enable the continuous culture of cell at high density and the production of valuable substance at high concentration over a long period.
JP62239167A 1987-09-24 1987-09-24 Cell culture device Expired - Lifetime JPH0797982B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62239167A JPH0797982B2 (en) 1987-09-24 1987-09-24 Cell culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62239167A JPH0797982B2 (en) 1987-09-24 1987-09-24 Cell culture device

Publications (2)

Publication Number Publication Date
JPS6485068A true JPS6485068A (en) 1989-03-30
JPH0797982B2 JPH0797982B2 (en) 1995-10-25

Family

ID=17040726

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62239167A Expired - Lifetime JPH0797982B2 (en) 1987-09-24 1987-09-24 Cell culture device

Country Status (1)

Country Link
JP (1) JPH0797982B2 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04148676A (en) * 1990-10-09 1992-05-21 Tabai Espec Corp Perfusion culture
JP2005261342A (en) * 2004-03-19 2005-09-29 Yanmar Co Ltd Plankton culture system
JP2009543565A (en) * 2006-07-14 2009-12-10 ディーエスエム アイピー アセッツ ビー．ブイ． Improved method of culturing cells
US8206981B2 (en) 2004-03-05 2012-06-26 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
CN109913410A (en) * 2019-04-19 2019-06-21 华子昂 The emulation cultural method of stem cell
JP2020089304A (en) * 2018-12-05 2020-06-11 株式会社日立ハイテク Cell culture apparatus, and method for controlling gas concentration in culture solution

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04148676A (en) * 1990-10-09 1992-05-21 Tabai Espec Corp Perfusion culture
US8206981B2 (en) 2004-03-05 2012-06-26 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
JP2005261342A (en) * 2004-03-19 2005-09-29 Yanmar Co Ltd Plankton culture system
JP2012165764A (en) * 2006-07-14 2012-09-06 Dsm Ip Assets Bv Improved process for culturing cell
US8119368B2 (en) 2006-07-14 2012-02-21 Dsm Ip Assets B.V. Process for the culturing of cells
US8222001B2 (en) 2006-07-14 2012-07-17 Dsm Ip Assets B.V. Process for the culturing of cells
JP2009543565A (en) * 2006-07-14 2009-12-10 ディーエスエム アイピー アセッツ ビー．ブイ． Improved method of culturing cells
US8440458B2 (en) 2006-07-14 2013-05-14 Dsm Ip Assets B.V. Process for the culturing of cells
TWI413689B (en) * 2006-07-14 2013-11-01 Dsm Ip Assets Bv Improved process for the culturing of cells
US8685731B2 (en) 2006-07-14 2014-04-01 Dsm Ip Assets B.V. Process for the culturing of cells
JP2014217395A (en) * 2006-07-14 2014-11-20 ディーエスエム アイピー アセッツ ビー．ブイ． Improved process for culturing cells
TWI512102B (en) * 2006-07-14 2015-12-11 Dpx Holdings Bv Improved process for the culturing of cells
US9670520B2 (en) 2006-07-14 2017-06-06 Patheon Holdings | B.V. Process for the culturing of cells
JP2020089304A (en) * 2018-12-05 2020-06-11 株式会社日立ハイテク Cell culture apparatus, and method for controlling gas concentration in culture solution
CN109913410A (en) * 2019-04-19 2019-06-21 华子昂 The emulation cultural method of stem cell

Also Published As

Publication number Publication date
JPH0797982B2 (en) 1995-10-25

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